Rroxscaffold_2G00107830

Potassium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001609
Physical Location & Seq
Forward (+)
31852935 .. 31857170
4236 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00107830.1

Sequence Viewer

Length: 435 bp
ATGGATCACAAGATTTCTCCTCAAAATCTCAAGGAGATTATTGAAGCCAAAAGCCTATATCGTGTGCGTGGCCTTGCCATGTTCTACTCTGAACTTGTTCATGGCATCACACCCATTTTCAATCACTATGTAGCCAATGTGCCTCCAACAGAGGAGGAGGATCACCGCATTGCTGCTCCATTGTTTCCACCGCGTCATGTCCTCTCGGCGACATGCCGTTCGACCACGACTTTGGCGGCCGGCTATTCCAACCATGCATCAACATCGAAGACCGTTGCTCCAAGCCAATCGGTCCACCACCACAATAGGCAACGATCGGATCGGCAACAATCGAAGTCCGGGCGACTCGCTCGCCGCCGATCAGCAATCGTCCAAGCCGGTGGCAAAACACAACCGGTTGGAACCAGCAACCTAAGCGCTACGAAGGAGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K_trans

Protein Analysis

144

Amino Acids

15.92

Weight (kDa)

10.22

Isoelectric Point (pI)

69.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 193
AciI CCGC 4 cut(s) 166, 191, 236, 355
AclWI GGATC 3 cut(s) 12, 168, 327
AcoI YGGCCR 1 cut(s) 237
AfeI AGCGCT 1 cut(s) 418
AgeI ACCGGT 1 cut(s) 394
AgsI TTSAA 2 cut(s) 44, 121
AlwI GGATC 3 cut(s) 12, 168, 327
Aor51HI AGCGCT 1 cut(s) 418
AoxI GGCC 2 cut(s) 70, 237
ApeKI GCWGC 1 cut(s) 173
ArsI GACNNNNNNTTYG 4 cut(s) 202, 214, 234, 246
AsiGI ACCGGT 1 cut(s) 394
Asp700I GAANNNNTTC 1 cut(s) 96
AspLEI GCGC 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 292
AsuC2I CCSGG 1 cut(s) 340
AsuHPI GGTGA 1 cut(s) 155
AvaII GGWCC 1 cut(s) 292
BbsI GAAGAC 1 cut(s) 275
BbvI GCAGC 1 cut(s) 160
BceAI ACGGC 1 cut(s) 201
BcgI CGANNNNNNTGC 2 cut(s) 246, 280
BcnI CCSGG 1 cut(s) 340
BfoI RGCGCY 1 cut(s) 420
BisI GCNGC 3 cut(s) 174, 237, 355
BlsI GCNGC 3 cut(s) 175, 238, 356
Bme1390I CCNGG 1 cut(s) 340
Bme18I GGWCC 1 cut(s) 292
BmgT120I GGNCC 1 cut(s) 292
BmiI GGNNCC 1 cut(s) 403
BmrFI CCNGG 1 cut(s) 340
BmsI GCATC 2 cut(s) 114, 266
BpiI GAAGAC 1 cut(s) 275
Bpu10I CCTNAGC 1 cut(s) 413
BpuEI CTTGAG 1 cut(s) 14
BpuMI CCSGG 1 cut(s) 340
BsaWI WCCGGW 1 cut(s) 394
BsaXI ACNNNNNCTCC 2 cut(s) 262, 292
Bse118I RCCGGY 3 cut(s) 239, 377, 394
Bse3DI GCAATG 1 cut(s) 168
BseMI GCAATG 1 cut(s) 168
BseRI GAGGAG 3 cut(s) 9, 167, 170
BseX3I CGGCCG 1 cut(s) 237
BseXI GCAGC 1 cut(s) 160
Bsh1236I CGCG 1 cut(s) 193
Bsh1285I CGRYCG 2 cut(s) 240, 317
BshFI GGCC 2 cut(s) 72, 239
BshTI ACCGGT 1 cut(s) 394
BsiEI CGRYCG 2 cut(s) 240, 317
BsiSI CCGG 4 cut(s) 240, 339, 378, 395
BsnI GGCC 2 cut(s) 72, 239
Bsp143I GATC 5 cut(s) 4, 160, 314, 319, 359
BspACI CCGC 4 cut(s) 166, 191, 236, 355
BspANI GGCC 2 cut(s) 72, 239
BspFNI CGCG 1 cut(s) 193
BspLI GGNNCC 1 cut(s) 403
BspPI GGATC 3 cut(s) 12, 168, 327
BsrDI GCAATG 1 cut(s) 168
BsrFI RCCGGY 3 cut(s) 239, 377, 394
BssAI RCCGGY 3 cut(s) 239, 377, 394
BssMI GATC 5 cut(s) 4, 160, 314, 319, 359
Bst4CI ACNGT 1 cut(s) 274
BstC8I GCNNGC 2 cut(s) 241, 352
BstDEI CTNAG 1 cut(s) 413
BstFNI CGCG 1 cut(s) 193
BstH2I RGCGCY 1 cut(s) 420
BstHHI GCGC 1 cut(s) 419
BstKTI GATC 5 cut(s) 7, 163, 317, 322, 362
BstMBI GATC 5 cut(s) 4, 160, 314, 319, 359
BstMCI CGRYCG 2 cut(s) 240, 317
BstMWI GCNNNNNNNGC 1 cut(s) 414
BstNSI RCATGY 1 cut(s) 216
BstSCI CCNGG 1 cut(s) 338
BstUI CGCG 1 cut(s) 193
BstV1I GCAGC 1 cut(s) 160
BstV2I GAAGAC 1 cut(s) 275
BstXI CCANNNNNNTGG 2 cut(s) 232, 380
BstZI CGGCCG 1 cut(s) 237
BsuRI GGCC 2 cut(s) 72, 239
Cac8I GCNNGC 2 cut(s) 241, 352
CfoI GCGC 1 cut(s) 419
Cfr10I RCCGGY 3 cut(s) 239, 377, 394
Cfr13I GGNCC 1 cut(s) 292
CseI GACGC 1 cut(s) 182
CspAI ACCGGT 1 cut(s) 394
CviAII CATG 5 cut(s) 79, 101, 197, 213, 254
CviJI RGCY 8 cut(s) 47, 54, 72, 134, 239, 243, 285, 377
CviKI_1 RGCY 8 cut(s) 47, 54, 72, 134, 239, 243, 285, 377
DdeI CTNAG 1 cut(s) 413
DpnI GATC 5 cut(s) 6, 162, 316, 321, 361
DpnII GATC 5 cut(s) 4, 160, 314, 319, 359
EaeI YGGCCR 1 cut(s) 237
EagI CGGCCG 1 cut(s) 237
EclXI CGGCCG 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 292
Eco47III AGCGCT 1 cut(s) 418
Eco52I CGGCCG 1 cut(s) 237
EcoT22I ATGCAT 1 cut(s) 259
FaeI CATG 5 cut(s) 82, 104, 200, 216, 257
FaiI YATR 7 cut(s) 58, 80, 102, 129, 198, 214, 255
FatI CATG 5 cut(s) 78, 100, 196, 212, 253
Fnu4HI GCNGC 3 cut(s) 174, 237, 355
Fsp4HI GCNGC 3 cut(s) 174, 237, 355
GlaI GCGC 1 cut(s) 418
GluI GCNGC 3 cut(s) 174, 237, 355
HaeII RGCGCY 1 cut(s) 420
HaeIII GGCC 2 cut(s) 72, 239
HapII CCGG 4 cut(s) 240, 339, 378, 395
HgaI GACGC 1 cut(s) 182
HhaI GCGC 1 cut(s) 419
Hin1II CATG 5 cut(s) 82, 104, 200, 216, 257
Hin6I GCGC 1 cut(s) 417
HinP1I GCGC 1 cut(s) 417
HinfI GANTC 1 cut(s) 345
HpaII CCGG 4 cut(s) 240, 339, 378, 395
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 2 cut(s) 91, 319
Hpy8I GTNNAC 1 cut(s) 295
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 1 cut(s) 274
HpyCH4V TGCA 1 cut(s) 257
HpyF10VI GCNNNNNNNGC 1 cut(s) 414
HpyF3I CTNAG 1 cut(s) 413
Hsp92II CATG 5 cut(s) 82, 104, 200, 216, 257
HspAI GCGC 1 cut(s) 417
KroI GCCGGC 1 cut(s) 239
KroNI GCCGGC 1 cut(s) 241
Kzo9I GATC 5 cut(s) 4, 160, 314, 319, 359
LmnI GCTCC 2 cut(s) 181, 283
LpnPI CCDG 5 cut(s) 253, 352, 391, 408, 418
Lsp1109I GCAGC 1 cut(s) 160
LweI GCATC 2 cut(s) 114, 266
MalI GATC 5 cut(s) 6, 162, 316, 321, 361
MboI GATC 5 cut(s) 4, 160, 314, 319, 359
MboII GAAGA 1 cut(s) 280
MlyI GAGTC 1 cut(s) 339
MmeI TCCRAC 3 cut(s) 170, 273, 379
MnlI CCTC 6 cut(s) 30, 145, 148, 151, 153, 212
Mph1103I ATGCAT 1 cut(s) 259
MroNI GCCGGC 1 cut(s) 239
MroXI GAANNNNTTC 1 cut(s) 96
MspI CCGG 4 cut(s) 240, 339, 378, 395
MspR9I CCNGG 1 cut(s) 340
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 414
NaeI GCCGGC 1 cut(s) 241
NciI CCSGG 1 cut(s) 340
NdeII GATC 5 cut(s) 4, 160, 314, 319, 359
NgoMIV GCCGGC 1 cut(s) 239
NlaIII CATG 5 cut(s) 82, 104, 200, 216, 257
NlaIV GGNNCC 1 cut(s) 403
NmeAIII GCCGAG 1 cut(s) 185
NsiI ATGCAT 1 cut(s) 259
NspI RCATGY 1 cut(s) 216
PdiI GCCGGC 1 cut(s) 241
PdmI GAANNNNTTC 1 cut(s) 96
PinAI ACCGGT 1 cut(s) 394
PkrI GCNGC 3 cut(s) 175, 238, 356
Ple19I CGATCG 1 cut(s) 317
PleI GAGTC 1 cut(s) 339
PpsI GAGTC 1 cut(s) 339
PspN4I GGNNCC 1 cut(s) 403
PspPI GGNCC 1 cut(s) 292
PvuI CGATCG 1 cut(s) 317
SatI GCNGC 3 cut(s) 174, 237, 355
Sau3AI GATC 5 cut(s) 4, 160, 314, 319, 359
Sau96I GGNCC 1 cut(s) 292
SchI GAGTC 1 cut(s) 339
ScrFI CCNGG 1 cut(s) 340
SetI ASST 1 cut(s) 414
SfaNI GCATC 2 cut(s) 114, 266
SinI GGWCC 1 cut(s) 292
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
SsiI CCGC 4 cut(s) 166, 191, 236, 355
StyD4I CCNGG 1 cut(s) 338
TaaI ACNGT 1 cut(s) 274
TaqI TCGA 3 cut(s) 221, 266, 332
TaqII GACCGA 1 cut(s) 280
TauI GCSGC 2 cut(s) 239, 357
TseI GCWGC 1 cut(s) 173
TspDTI ATGAA 1 cut(s) 89
VpaK11BI GGWCC 1 cut(s) 292
XceI RCATGY 1 cut(s) 216
XmnI GAANNNNTTC 1 cut(s) 96
Zsp2I ATGCAT 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.