Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr6

Orthogroup ID

OG0001609

Physical Location & Seq

Genomic Coordinates Reverse (-)

54388175 .. 54394649

Gene Structure 6475 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw6G030180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2436 bp

ATGCCTGAAGATGCAATATTAGAAAACGGTACAGATCAGGATCCTAAAAGCCATCACCGCCAAGAAGTAGTAGTACTTCCTCATCATCCGGATCATCAACAACTCAAAGGCAAGAAACTTTCATGGCAAAAGTTACGTAGATTTGACTCCTTGGACATCGAGTCACGCAGTGTCAACGGTCACCATGGCCATAGCTCCCAGGGTGCAAGATGGTCGGTGATACTGCACCTAGCATTTCAGAGCCTTGGAATAGTCTATGGAGACATTGGTACATCACCTCTATATGTGTATGCGAGTACCTTCACCAATGGCATCAAGGACATAGATGACATCTTGGGTGTTCTTTCTTTGATCATATACACCATCACCTTAATCCCCTTGATTAAGTACGTCTTCATCGTCCTCCGAGCCAACGATAATGGCGACGGAGGGACGTTCGCACTTTACTCTCTGTTGTGTCGATATGCCAAGGTTGGTTTGACTCCGAGTCAGCAAGCCGAAGACCGCGATGTTTCAAATTTCCAACTTGAGTTGCCTAGCAAACGCCTAAGGAGGGCATCGAAGCTTAAATCAAAATTAGAGAACAGCAGATTTGCCAAGTACTTTTTACTATTCGCCACCATGCTTGGTACTTCTATGGTTATTGGTGATGGCGTCCTCACTCCTTGCATCTCAGTTCTATCGGCTGTTGGAGGGATGAAACAAGCTACATCTGCCCTGACCGACGATATGGTTGTTTGGATATCAGTAGGCATCTTGGTTTGCCTATTCATGGTTCAGAGATTTGGAACTGACAAAGTTGGCTACAGTTTTGCACCAATAATTTGTGTTTGGTTTACCTTCATTGGTGGCATTGGCGTCTACAATTTCTTCAAATACGATCCAACAGTTTTGAAAGCTCTAAATCCACGATACATAGTCGATTACTTCAGGAGAAACAAAGAAGATGCTTGGATTTCCCTCGGTGGCATTGTTCTTGCCATTACAGGAACTGAAGCCCTGTTTGCCGATGTTGGCCACTTCACAGTTCGATCCATTCAGATTAGTATGTGCTCAGTGACTTACCCAGCTCTCCTACTTGCATATACCGGACAAGCCTCTTTTCTGCGTGAGCACAAGACTCTAGTTTCGAACACCTTCTATGAGTCCATACCAAATCCTTTGTATTGGCCTATGTTTGGGGTTGCCGTAATGGCATCAATCATAGCCAGCCAAGCCATGATTTCGGGTACCTTCTCAATCATCCAACAGTCTCTTTCACTAGGGTGTTTCCCTCGTGTGAAAATTGTGCACACATCGACCAAGTATGAAGGACAAGTTTACATTCCGGAGGTCAACTACCTTCTCATGCTGGCTTGTGTTGGAGTCACCTTGGGTTTCAGAAGCACCACAAAGATTGGAAATGCATACGGCATAGCAGTGGTATTTGTAATGACCCTTACGTCGTCCTTCCTGGTGCTAATCATGATTATGATTTGGAAGACCCCATTACTTCTCATTATCTCATATGTTCTTATCATCGGAAGCGTGGAGCTTATGTACTTGAGTTCTGTGCTCTACAAATTTGACCAAGGTGGATATCTTCCTTTGGCATTTGCCACTTTACTGATGCTCATAATGTTTGTGTGGAACGATGTTCATCGAAGAAAGTACTACTACGAGCTCGAGCATAAAATTTCCCCTGAAAAGCTCAAGCAACTTGCCGTGGACAAAAACTTTTGCCGCATGCCTGGCCTCGCCATGTTCTATTCAGAACTCGTTCAAGGAATTCCTCCAATCTTCAGTCATTACGCGGCAAATGTGCCTGCTTTGCACTCAGTGCTGGTTTTTGTCTCCATCAAATCATTACCGATAAGCAAGGTTCCGTTGGAGGAGCGTTTTCTTTTCCGCAGAGTGGAGCCTAGAGAGCTTCATGTGTTCCGATGTGTCGCTAGATACGGGTACACAGATGTCCGAAATGAGAATGAACCCTTTGAAGGATTGCTGATAGAAAAATTGAAGGAGTTCATAAGGGATGACTTTTGGTTATCTCAAAGAACCATGCACATAACCGATGGAGAGAAATTGGAAAAGGAAGTTTCTGCTGAGTTAGAAGGTGATGATCATGAGTTGGTTAATGGAAATGGTCATAATGAGAATGAGAACGAAAACGAGAATGTAAAGCAAGTACTTGATGATGATGAAGATCAAAGGAAACAACAAGACGATGTACTGTTGGAGACAGAGATTGAGGCGATTGACAAAGCGTGGCATCGGGGGGTTGTGCACTTAATTGGTGAGAATGAAGTGACGGCTGCGAAAGGAGCTGGGATAGCGAAAAGGATCTTGATTGATTATGCTTACAATTTCTTGAAGAGAAATCTGAGGCAGAGCGACAAAGTGTTTGATATTCCTCACAAGCGCATGTTAAAAGTGGGCATGACTTATGAACTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

811

Amino Acids

91.72

Weight (kDa)

6.77

Isoelectric Point (pI)

33.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	77 - 563	3.1e-168	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	577 - 811	1.8e-26	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001609)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g30541 FvH4_6g30550 FvH4_c6g00050 FvH4_c6g00050 FvH4_c6g00050
malus_domestica	MD03G1283500.v1.1 MD03G1283600.v1.1 MD10G1230200.v1.1 MD10G1230300.v1.1 MD11G1302600.v1.1 MD11G1302900.v1.1 MD11G1303100.v1.1
prunus_persica	Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1
pyrus_communis	pycom03g22450 pycom03g22460
rosa_chinensis	RchiOBHm_Chr2g0136981 RchiOBHm_Chr5g0020481 RchiOBHm_Chr6g0293251
rosa_laevigata	RLG00000011964 RLG00000033905
rosa_multiflora	Rmu_co8314359.1_g000001 Rmu_sc0001374.1_g000063 Rmu_sc0001374.1_g000064 Rmu_sc0021490.1_g000001 Rmu_ssc0000162.1_g000030
rosa_roxburghii	Rroxscaffold_2G00107800 Rroxscaffold_2G00107830 Rroxscaffold_7G00174310
rosa_rugosa	Rorug02G0336400 Rorug06G0233800 Rorug06G0233800 Rorug06G0233800
rosa_samantha	Rh2AG385800 Rh2BG392700 Rh2CG373000 Rh2DG047100 Rh2DG408800 Rh2DG408900 Rh3DG241600 Rh3DG283500 Rh6AG346700 Rh6BG354100 Rh6CG360600 Rh6DG347100
rosa_wichuraiana	Rw2G003360 Rw2G031570 Rw6G030180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1441
Acc65I	GGTACC	1 cut(s)	1229
AccB1I	GGYRCC	1 cut(s)	1229
AccI	GTMKAC	1 cut(s)	861
AccII	CGCG	2 cut(s)	507, 1793
AccIII	TCCGGA	2 cut(s)	88, 1327
AciI	CCGC	5 cut(s)	58, 505, 1723, 1793, 1888
AclWI	GGATC	6 cut(s)	35, 48, 99, 875, 1026, 2330
AcoI	YGGCCR	2 cut(s)	187, 1015
AcsI	RAATTY	4 cut(s)	517, 1562, 1674, 1767
AcuI	CTGAAG	4 cut(s)	27, 913, 1014, 1765
AcyI	GRCGYC	2 cut(s)	654, 858
AdeI	CACNNNGTG	2 cut(s)	170, 1819
AfiI	CCNNNNNNNGG	5 cut(s)	553, 772, 1178, 1894, 1976
AgsI	TTSAA	7 cut(s)	516, 874, 895, 1763, 1976, 1999, 2353
AhdI	GACNNNNNGTC	2 cut(s)	160, 486
AjnI	CCWGG	3 cut(s)	198, 1452, 1729
AleI	CACNNNNGTG	1 cut(s)	1264
Alw21I	GWGCWC	6 cut(s)	1055, 1116, 1293, 1557, 1665, 2268
Alw26I	GTCTC	4 cut(s)	255, 1257, 1837, 2213
Alw44I	GTGCAC	2 cut(s)	1289, 2264
AlwI	GGATC	6 cut(s)	35, 48, 99, 875, 1026, 2330
AlwNI	CAGNNNCTG	1 cut(s)	992
Ama87I	CYCGRG	1 cut(s)	1664
Aor13HI	TCCGGA	2 cut(s)	88, 1327
AoxI	GGCC	4 cut(s)	187, 1015, 1169, 1732
ApaLI	GTGCAC	2 cut(s)	1289, 2264
ApeKI	GCWGC	1 cut(s)	2294
ApoI	RAATTY	4 cut(s)	517, 1562, 1674, 1767
ArsI	GACNNNNNNTTYG	4 cut(s)	1111, 1143, 2366, 2398
Asp700I	GAANNNNTTC	3 cut(s)	1136, 1758, 2003
Asp718I	GGTACC	1 cut(s)	1229
AspLEI	GCGC	1 cut(s)	2403
AsuII	TTCGAA	1 cut(s)	1130
AvaI	CYCGRG	1 cut(s)	1664
AxyI	CCTNAGG	1 cut(s)	548
BaeGI	GKGCMC	2 cut(s)	1293, 2268
BalI	TGGCCA	2 cut(s)	189, 1017
BamHI	GGATCC	1 cut(s)	40
BanI	GGYRCC	1 cut(s)	1229
BanII	GRGCYC	1 cut(s)	1665
BarI	GAAGNNNNNNTAC	4 cut(s)	57, 60, 89, 92
BauI	CACGAG	1 cut(s)	1275
BbsI	GAAGAC	3 cut(s)	385, 507, 1487
Bbv12I	GWGCWC	6 cut(s)	1055, 1116, 1293, 1557, 1665, 2268
BbvI	GCAGC	1 cut(s)	2281
BccI	CCATC	6 cut(s)	60, 204, 371, 644, 1844, 2048
BceAI	ACGGC	4 cut(s)	1172, 1426, 1688, 2307
BcgI	CGANNNNNNTGC	2 cut(s)	195, 229
BciT130I	CCWGG	3 cut(s)	200, 1454, 1731
BclI	TGATCA	2 cut(s)	351, 2101
BcoDI	GTCTC	4 cut(s)	255, 1257, 1837, 2213
BfaI	CTAG	6 cut(s)	230, 537, 1124, 1262, 1902, 1932
BfmI	CTRYAG	2 cut(s)	805, 2432
BglI	GCCNNNNNGGC	1 cut(s)	1193
BisI	GCNGC	3 cut(s)	1723, 1794, 2295
BlsI	GCNGC	3 cut(s)	1724, 1795, 2296
BmcAI	AGTACT	4 cut(s)	75, 602, 1652, 2169
Bme1390I	CCNGG	3 cut(s)	200, 1454, 1731
BmeRI	GACNNNNNGTC	2 cut(s)	160, 486
BmeT110I	CYCGRG	1 cut(s)	1664
BmiI	GGNNCC	4 cut(s)	42, 1231, 1863, 1899
BmrFI	CCNGG	3 cut(s)	200, 1454, 1731
BmsI	GCATC	8 cut(s)	321, 566, 678, 762, 937, 1205, 1599, 2260
BpiI	GAAGAC	3 cut(s)	385, 507, 1487
Bpu14I	TTCGAA	1 cut(s)	1130
BpuEI	CTTGAG	3 cut(s)	548, 1564, 1676
BsaAI	YACGTR	1 cut(s)	137
BsaBI	GATNNNNATC	3 cut(s)	39, 1638, 2184
BsaHI	GRCGYC	2 cut(s)	654, 858
BsaWI	WCCGGW	3 cut(s)	88, 1088, 1327
BsaXI	ACNNNNNCTCC	2 cut(s)	1523, 1553
Bsc4I	CCNNNNNNNGG	5 cut(s)	553, 772, 1178, 1894, 1976
Bse21I	CCTNAGG	1 cut(s)	548
Bse8I	GATNNNNATC	3 cut(s)	39, 1638, 2184
BseAI	TCCGGA	2 cut(s)	88, 1327
BseBI	CCWGG	3 cut(s)	200, 1454, 1731
BseGI	GGATG	4 cut(s)	85, 702, 1242, 2020
BseJI	GATNNNNATC	3 cut(s)	39, 1638, 2184
BseLI	CCNNNNNNNGG	5 cut(s)	553, 772, 1178, 1894, 1976
BseMII	CTCAG	5 cut(s)	687, 1068, 1830, 2076, 2354
BseRI	GAGGAG	1 cut(s)	1886
BseSI	GKGCMC	2 cut(s)	1293, 2268
BseXI	GCAGC	1 cut(s)	2281
BseYI	CCCAGC	2 cut(s)	1066, 2306
BsgI	GTGCAG	1 cut(s)	209
Bsh1236I	CGCG	2 cut(s)	507, 1793
BshFI	GGCC	4 cut(s)	189, 1017, 1171, 1734
BshNI	GGYRCC	1 cut(s)	1229
BsiHKAI	GWGCWC	6 cut(s)	1055, 1116, 1293, 1557, 1665, 2268
BsiHKCI	CYCGRG	1 cut(s)	1664
BsiSI	CCGG	3 cut(s)	89, 1089, 1328
BslFI	GGGAC	1 cut(s)	445
BslI	CCNNNNNNNGG	5 cut(s)	553, 772, 1178, 1894, 1976
BsmAI	GTCTC	4 cut(s)	255, 1257, 1837, 2213
BsmFI	GGGAC	1 cut(s)	445
BsnI	GGCC	4 cut(s)	189, 1017, 1171, 1734
BsoBI	CYCGRG	1 cut(s)	1664
Bsp119I	TTCGAA	1 cut(s)	1130
Bsp1286I	GDGCHC	6 cut(s)	1055, 1116, 1293, 1557, 1665, 2268
Bsp13I	TCCGGA	2 cut(s)	88, 1327
Bsp143I	GATC	9 cut(s)	34, 40, 91, 351, 880, 1031, 2101, 2185, 2322
Bsp19I	CCATGG	1 cut(s)	184
BspACI	CCGC	5 cut(s)	58, 505, 1723, 1793, 1888
BspANI	GGCC	4 cut(s)	189, 1017, 1171, 1734
BspCNI	CTCAG	5 cut(s)	686, 1067, 1829, 2077, 2355
BspEI	TCCGGA	2 cut(s)	88, 1327
BspFNI	CGCG	2 cut(s)	507, 1793
BspHI	TCATGA	2 cut(s)	1464, 2104
BspLI	GGNNCC	4 cut(s)	42, 1231, 1863, 1899
BspPI	GGATC	6 cut(s)	35, 48, 99, 875, 1026, 2330
BspT104I	TTCGAA	1 cut(s)	1130
BspT107I	GGYRCC	1 cut(s)	1229
BssMI	GATC	9 cut(s)	34, 40, 91, 351, 880, 1031, 2101, 2185, 2322
BssNI	GRCGYC	2 cut(s)	654, 858
BssSI	CACGAG	1 cut(s)	1275
BssT1I	CCWWGG	6 cut(s)	150, 184, 244, 468, 1371, 1570
Bst2BI	CACGAG	1 cut(s)	1275
Bst2UI	CCWGG	3 cut(s)	200, 1454, 1731
Bst4CI	ACNGT	7 cut(s)	29, 179, 809, 889, 1027, 1251, 2214
Bst6I	CTCTTC	1 cut(s)	2348
BstACI	GRCGYC	2 cut(s)	654, 858
BstAPI	GCANNNNNTGC	1 cut(s)	1819
BstBAI	YACGTR	1 cut(s)	137
BstBI	TTCGAA	1 cut(s)	1130
BstC8I	GCNNGC	5 cut(s)	495, 1210, 1353, 1727, 1806
BstDEI	CTNAG	6 cut(s)	548, 673, 1054, 1816, 2085, 2363
BstDSI	CCRYGG	2 cut(s)	184, 1704
BstEII	GGTNACC	1 cut(s)	179
BstF5I	GGATG	4 cut(s)	85, 702, 1242, 2020
BstFNI	CGCG	2 cut(s)	507, 1793
BstHHI	GCGC	1 cut(s)	2403
BstKTI	GATC	9 cut(s)	37, 43, 94, 354, 883, 1034, 2104, 2188, 2325
BstMAI	GTCTC	4 cut(s)	255, 1257, 1837, 2213
BstMBI	GATC	9 cut(s)	34, 40, 91, 351, 880, 1031, 2101, 2185, 2322
BstNI	CCWGG	3 cut(s)	200, 1454, 1731
BstNSI	RCATGY	2 cut(s)	1729, 2407
BstPI	GGTNACC	1 cut(s)	179
BstSCI	CCNGG	3 cut(s)	198, 1452, 1729
BstSFI	CTRYAG	2 cut(s)	805, 2432
BstSLI	GKGCMC	2 cut(s)	1293, 2268
BstSNI	TACGTA	1 cut(s)	137
BstUI	CGCG	2 cut(s)	507, 1793
BstV1I	GCAGC	1 cut(s)	2281
BstV2I	GAAGAC	3 cut(s)	385, 507, 1487
BstX2I	RGATCY	2 cut(s)	40, 2322
BstYI	RGATCY	2 cut(s)	40, 2322
Bsu36I	CCTNAGG	1 cut(s)	548
BsuRI	GGCC	4 cut(s)	189, 1017, 1171, 1734
BtgI	CCRYGG	2 cut(s)	184, 1704
BtgZI	GCGATG	1 cut(s)	522
BtsCI	GGATG	4 cut(s)	85, 702, 1242, 2020
BtsI	GCAGTG	2 cut(s)	175, 1425
BtsIMutI	CAGTG	4 cut(s)	175, 1062, 1425, 1824
Cac8I	GCNNGC	5 cut(s)	495, 1210, 1353, 1727, 1806
CaiI	CAGNNNCTG	1 cut(s)	992
CciI	TCATGA	2 cut(s)	1464, 2104
CfoI	GCGC	1 cut(s)	2403
CseI	GACGC	2 cut(s)	643, 847
CspCI	CAANNNNNGTGG	4 cut(s)	607, 642, 1378, 1413
DdeI	CTNAG	6 cut(s)	548, 673, 1054, 1816, 2085, 2363
DpnI	GATC	9 cut(s)	36, 42, 93, 353, 882, 1033, 2103, 2187, 2324
DpnII	GATC	9 cut(s)	34, 40, 91, 351, 880, 1031, 2101, 2185, 2322
DraIII	CACNNNGTG	2 cut(s)	170, 1819
DrdI	GACNNNNNNGTC	1 cut(s)	1441
DriI	GACNNNNNGTC	2 cut(s)	160, 486
DseDI	GACNNNNNNGTC	1 cut(s)	1441
EaeI	YGGCCR	2 cut(s)	187, 1015
Eam1104I	CTCTTC	1 cut(s)	2348
Eam1105I	GACNNNNNGTC	2 cut(s)	160, 486
EarI	CTCTTC	1 cut(s)	2348
Ecl136II	GAGCTC	1 cut(s)	1663
Eco105I	TACGTA	1 cut(s)	137
Eco130I	CCWWGG	6 cut(s)	150, 184, 244, 468, 1371, 1570
Eco24I	GRGCYC	1 cut(s)	1665
Eco32I	GATATC	2 cut(s)	744, 1580
Eco53kI	GAGCTC	1 cut(s)	1663
Eco57I	CTGAAG	4 cut(s)	27, 913, 1014, 1765
Eco81I	CCTNAGG	1 cut(s)	548
Eco88I	CYCGRG	1 cut(s)	1664
Eco91I	GGTNACC	1 cut(s)	179
EcoICRI	GAGCTC	1 cut(s)	1663
EcoO65I	GGTNACC	1 cut(s)	179
EcoRI	GAATTC	1 cut(s)	1767
EcoRII	CCWGG	3 cut(s)	198, 1452, 1729
EcoRV	GATATC	2 cut(s)	744, 1580
EcoT14I	CCWWGG	6 cut(s)	150, 184, 244, 468, 1371, 1570
EcoT22I	ATGCAT	1 cut(s)	1408
EcoT38I	GRGCYC	1 cut(s)	1665
ErhI	CCWWGG	6 cut(s)	150, 184, 244, 468, 1371, 1570
FalI	AAGNNNNNCTT	2 cut(s)	377, 409
FaqI	GGGAC	1 cut(s)	445
FauNDI	CATATG	1 cut(s)	1507
FbaI	TGATCA	2 cut(s)	351, 2101
FblI	GTMKAC	1 cut(s)	861
Fnu4HI	GCNGC	3 cut(s)	1723, 1794, 2295
FokI	GGATG	4 cut(s)	72, 709, 1229, 2027
FriOI	GRGCYC	1 cut(s)	1665
Fsp4HI	GCNGC	3 cut(s)	1723, 1794, 2295
FspBI	CTAG	6 cut(s)	230, 537, 1124, 1262, 1902, 1932
GlaI	GCGC	1 cut(s)	2402
GluI	GCNGC	3 cut(s)	1723, 1794, 2295
GsaI	CCCAGC	2 cut(s)	1070, 2310
HaeIII	GGCC	4 cut(s)	189, 1017, 1171, 1734
HapII	CCGG	3 cut(s)	89, 1089, 1328
HgaI	GACGC	2 cut(s)	643, 847
HhaI	GCGC	1 cut(s)	2403
Hin1I	GRCGYC	2 cut(s)	654, 858
Hin6I	GCGC	1 cut(s)	2401
HinP1I	GCGC	1 cut(s)	2401
HincII	GTYRAC	2 cut(s)	175, 1336
HindII	GTYRAC	2 cut(s)	175, 1336
HindIII	AAGCTT	1 cut(s)	563
HinfI	GANTC	7 cut(s)	146, 161, 481, 487, 1120, 1145, 1365
HpaII	CCGG	3 cut(s)	89, 1089, 1328
Hpy166II	GTNNAC	9 cut(s)	175, 837, 862, 1291, 1321, 1336, 1708, 1944, 2266
Hpy188III	TCNNGA	8 cut(s)	38, 89, 931, 1328, 1465, 2105, 2326, 2350
Hpy8I	GTNNAC	9 cut(s)	175, 837, 862, 1291, 1321, 1336, 1708, 1944, 2266
Hpy99I	CGWCG	3 cut(s)	428, 728, 1447
HpyCH4III	ACNGT	7 cut(s)	29, 179, 809, 889, 1027, 1251, 2214
HpyCH4IV	ACGT	4 cut(s)	136, 390, 434, 1442
HpyF3I	CTNAG	6 cut(s)	548, 673, 1054, 1816, 2085, 2363
HpySE526I	ACGT	4 cut(s)	136, 390, 434, 1442
Hsp92I	GRCGYC	2 cut(s)	654, 858
HspAI	GCGC	1 cut(s)	2401
Kpn2I	TCCGGA	2 cut(s)	88, 1327
KpnI	GGTACC	1 cut(s)	1233
Ksp22I	TGATCA	2 cut(s)	351, 2101
Kzo9I	GATC	9 cut(s)	34, 40, 91, 351, 880, 1031, 2101, 2185, 2322
LmnI	GCTCC	5 cut(s)	200, 1531, 1873, 1897, 2303
Lsp1109I	GCAGC	1 cut(s)	2281
LweI	GCATC	8 cut(s)	321, 566, 678, 762, 937, 1205, 1599, 2260
MaeI	CTAG	6 cut(s)	230, 537, 1124, 1262, 1902, 1932
MaeII	ACGT	4 cut(s)	136, 390, 434, 1442
MaeIII	GTNAC	6 cut(s)	132, 162, 179, 1057, 1366, 2287
MalI	GATC	9 cut(s)	36, 42, 93, 353, 882, 1033, 2103, 2187, 2324
MboI	GATC	9 cut(s)	34, 40, 91, 351, 880, 1031, 2101, 2185, 2322
MflI	RGATCY	2 cut(s)	40, 2322
MhlI	GDGCHC	6 cut(s)	1055, 1116, 1293, 1557, 1665, 2268
MlsI	TGGCCA	2 cut(s)	189, 1017
MluNI	TGGCCA	2 cut(s)	189, 1017
MlyI	GAGTC	7 cut(s)	140, 170, 475, 496, 1114, 1154, 1374
MmeI	TCCRAC	7 cut(s)	547, 670, 908, 1270, 1342, 1848, 2196
Mox20I	TGGCCA	2 cut(s)	189, 1017
Mph1103I	ATGCAT	1 cut(s)	1408
MroI	TCCGGA	2 cut(s)	88, 1327
MroXI	GAANNNNTTC	3 cut(s)	1136, 1758, 2003
MscI	TGGCCA	2 cut(s)	189, 1017
MseI	TTAA	6 cut(s)	371, 384, 567, 2115, 2270, 2408
MslI	CAYNNNNRTG	3 cut(s)	1264, 1418, 1469
Msp20I	TGGCCA	2 cut(s)	189, 1017
MspI	CCGG	3 cut(s)	89, 1089, 1328
MspR9I	CCNGG	3 cut(s)	200, 1454, 1731
MvaI	CCWGG	3 cut(s)	200, 1454, 1731
MvnI	CGCG	2 cut(s)	507, 1793
NcoI	CCATGG	1 cut(s)	184
NdeI	CATATG	1 cut(s)	1507
NdeII	GATC	9 cut(s)	34, 40, 91, 351, 880, 1031, 2101, 2185, 2322
NlaIV	GGNNCC	4 cut(s)	42, 1231, 1863, 1899
NmuCI	GTSAC	5 cut(s)	162, 179, 1057, 1366, 2287
NsiI	ATGCAT	1 cut(s)	1408
NspI	RCATGY	2 cut(s)	1729, 2407
NspV	TTCGAA	1 cut(s)	1130
OliI	CACNNNNGTG	1 cut(s)	1264
PaeI	GCATGC	1 cut(s)	1729
PaeR7I	CTCGAG	1 cut(s)	1664
PagI	TCATGA	2 cut(s)	1464, 2104
PasI	CCCWGGG	1 cut(s)	199
PcsI	WCGNNNNNNNCGW	2 cut(s)	396, 420
PdmI	GAANNNNTTC	3 cut(s)	1136, 1758, 2003
PkrI	GCNGC	3 cut(s)	1724, 1795, 2296
PleI	GAGTC	7 cut(s)	140, 169, 475, 495, 1114, 1153, 1373
PpsI	GAGTC	7 cut(s)	140, 169, 475, 495, 1114, 1153, 1373
Ppu21I	YACGTR	1 cut(s)	137
Psp124BI	GAGCTC	1 cut(s)	1665
Psp6I	CCWGG	3 cut(s)	198, 1452, 1729
PspEI	GGTNACC	1 cut(s)	179
PspFI	CCCAGC	2 cut(s)	1066, 2306
PspGI	CCWGG	3 cut(s)	198, 1452, 1729
PspN4I	GGNNCC	4 cut(s)	42, 1231, 1863, 1899
PspXI	VCTCGAGB	1 cut(s)	1664
PstNI	CAGNNNCTG	1 cut(s)	992
PsuI	RGATCY	2 cut(s)	40, 2322
RseI	CAYNNNNRTG	3 cut(s)	1264, 1418, 1469
SacI	GAGCTC	1 cut(s)	1665
SaqAI	TTAA	6 cut(s)	371, 384, 567, 2115, 2270, 2408
SatI	GCNGC	3 cut(s)	1723, 1794, 2295
Sau3AI	GATC	9 cut(s)	34, 40, 91, 351, 880, 1031, 2101, 2185, 2322
ScaI	AGTACT	4 cut(s)	75, 602, 1652, 2169
SchI	GAGTC	7 cut(s)	140, 170, 475, 496, 1114, 1154, 1374
ScrFI	CCNGG	3 cut(s)	200, 1454, 1731
SduI	GDGCHC	6 cut(s)	1055, 1116, 1293, 1557, 1665, 2268
SfaNI	GCATC	8 cut(s)	321, 566, 678, 762, 937, 1205, 1599, 2260
SfcI	CTRYAG	2 cut(s)	805, 2432
Sfr274I	CTCGAG	1 cut(s)	1664
SfuI	TTCGAA	1 cut(s)	1130
SlaI	CTCGAG	1 cut(s)	1664
SmiMI	CAYNNNNRTG	3 cut(s)	1264, 1418, 1469
SmlI	CTYRAG	4 cut(s)	527, 1543, 1664, 1691
SmoI	CTYRAG	4 cut(s)	527, 1543, 1664, 1691
SnaBI	TACGTA	1 cut(s)	137
SphI	GCATGC	1 cut(s)	1729
SsiI	CCGC	5 cut(s)	58, 505, 1723, 1793, 1888
SspI	AATATT	1 cut(s)	18
SspMI	CTAG	6 cut(s)	230, 537, 1124, 1262, 1902, 1932
SstI	GAGCTC	1 cut(s)	1665
StyD4I	CCNGG	3 cut(s)	198, 1452, 1729
StyI	CCWWGG	6 cut(s)	150, 184, 244, 468, 1371, 1570
TaaI	ACNGT	7 cut(s)	29, 179, 809, 889, 1027, 1251, 2214
TaiI	ACGT	4 cut(s)	139, 393, 437, 1445
TaqI	TCGA	9 cut(s)	159, 460, 560, 921, 1030, 1130, 1298, 1642, 1665
TaqII	GACCGA	1 cut(s)	737
TatI	WGTACW	6 cut(s)	73, 600, 1539, 1650, 2167, 2209
TauI	GCSGC	2 cut(s)	1725, 1796
Tru1I	TTAA	6 cut(s)	371, 384, 567, 2115, 2270, 2408
Tru9I	TTAA	6 cut(s)	371, 384, 567, 2115, 2270, 2408
TscAI	CASTG	4 cut(s)	175, 1062, 1425, 1824
TseFI	GTSAC	5 cut(s)	162, 179, 1057, 1366, 2287
TseI	GCWGC	1 cut(s)	2294
Tsp45I	GTSAC	5 cut(s)	162, 179, 1057, 1366, 2287
TspGWI	ACGGA	2 cut(s)	441, 1854
TspRI	CASTG	4 cut(s)	175, 1062, 1425, 1824
VneI	GTGCAC	2 cut(s)	1289, 2264
XapI	RAATTY	4 cut(s)	517, 1562, 1674, 1767
XceI	RCATGY	2 cut(s)	1729, 2407
XhoI	CTCGAG	1 cut(s)	1664
XmiI	GTMKAC	1 cut(s)	861
XmnI	GAANNNNTTC	3 cut(s)	1136, 1758, 2003
XspI	CTAG	6 cut(s)	230, 537, 1124, 1262, 1902, 1932
ZrmI	AGTACT	4 cut(s)	75, 602, 1652, 2169
Zsp2I	ATGCAT	1 cut(s)	1408

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.