Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001609

Physical Location & Seq

Genomic Coordinates Forward (+)

36360734 .. 36365613

Gene Structure 4880 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1283500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2268 bp

ATGATATTATTTTACAATTACTCCTTGCTTTTAACGCTTTTATATATTGATAGTAGTTATAACGTTCTAGTGGATGAGGACTGGCTGGTGACAATGCACCTGGCATTTCAGAGCATTGGGGTAGTTTTTGGGGACCTCGGTACATCACCTCTCTATATGTATGCGAGCATCTTCAACAAAGGCATCAACCATGACGATGACATTTTGGGTGTTCTTTCCTTGCTCTTTTACATTCTCACCTTAATCCCTCTGATTAAGTACACTTTTATCGTCTTACGGGCCACCGATAATGGCGAAGGGACGTTCGCACTATACTCTTTGCTGTGTCGATACGCCAAGATTGGTATGACTCCGAGTCAACAACCAGAGGATCGTGATGTTTCAAATTTTGAGCTCGAGTTCCCTACCAAAAGTGTAAAGAGGGCATCAAGGCTTAAATCTTTATTAGAGAACAGCCCATCCGCTAAAGTCTTTCTATTATTCGCCACCATGCTTGGTACTTCCATGGTCATTGGCGATGGTATCCTCACTCCTTCCCTCTCAGTTCTGTCGGCTGTGGTAGGAATCAAACAAGCTACATCTGCCATGACGGATGACCGGATTGTTTGGATATCAGTAGCCATCTTGATTGGCCTATTCATGGTTCAAAGATTTGGAACTGATAAAATAGGCTACAGTTTTGCACCAACTATTTGCATTTGGTTTATCATGGTTGCTGGAATTGGCGTCTATAACTTCATCAAGTTTGATCCGACGGTGGTCAAGGCTCTAAACCCAAAATACATAGTAGATTACTTCAGAAGGAACAAACATGATGCTTGGATTTCTATTGGTGGCATTGTCTTAGCCATAACAGGAACTGAAGCTTTATTTGCTGACGTGGGACACTTCACAGTACGGTCCATTCAACTAAGTATGTGCACAATTACTTATCCGGCTCTCGTATTGACATACGCTGGACAAGCTTCTTTTCTTCGCAAGAACCGTCTTCTTGTTGCAGATACCTTCTTCAAGTCGATACCAAAACCTTTGTATTGGCCTATGTTTGTAGTGAGTGTACTGTCATCGATCATATCTAGTCAAGCTATGATTTCAGGGACTTTCTCTATAATCCAACAATCACTATCATTAGGGTGTTTCCCTCGTGTGAAAATCGTGCACACATCGACCAAGTATGCCGGACAAGTTTACATTCCGGAAGTGAACTACCTTCTCATGTTAGCTTGTGTTGGAGTCACCTTAGGGTTTCGAACCCTTGAAAGGATCGGCAATGCATATGGTATAGCAGTGGTGTTTGTAATGACGCTTACATCATCCTTCCTAGTGCTAATCATGATCATGATATGGAAGACCAACATATTCCTCATCATCTCATATGTTCTTGTCATTGGAAGTGTAGAGTTTCTGTGTCTAAGTTCAATGCTGTACAAATTTGACCAGGGCGGATATCTTCCCCTCGCGTTTGCCGCAGTGTTGATGTTTGTAATGTTTGTGTGGAATGATGTGCATCGAAGAAAGTACTATTACGAGCTTGATCACAAAATTTCTCCTGAACAGCTCAAGAAAACCGCCATTGACAGAAATTTTTGCCGCATGCCTGGCCTTGCCATCTTTTATTCCGAAATCGTTCAAGGAATCCCGCCGATCTTCGATCATTACGCTGCGAATGTTCCTGCATTGCACTCCGTCCTTGTTTTTGTCTCGATCAAATCATTGCCTATAAGCAAGGTTCCGCTAGAAGAGCGCTTTCTTTTTCGGAGAGTGGAGCCTAAGGATCTGAATGTGTTCCGATGTGTTGCAAGATACGGGTACACGGATGTTCGCAATGAGAATGAACCCTTCGAAGGATTGTTGGTGGAAAAAATGAAAGAGTTCATAAGGGACAGTTTTTGGATATCTCAAAGAAATAATATGCACAGTTCTGATGGGGAGATCAAGTTTGGGATCAAGGAAGAAGTCCAAGACTGGACTTTGGTGAACAGTGGAGAGAATGGAAAGGAGGATTCAAAGCGGCAAGTTGATGATCAAGATAAGCAACAAGATTTGTTGGACAGAGAGATTGAGGCAATAGACAAAGCATGGAGGCAGGGAGTAGTTCATTTGATTGGGGAAAATGAAGTGACGGCTGCCGAAGGAGCTGGTATAGTGAGAAGACTTTTGATTGATTATGCTTACAATTTCTTGAAGAGAAATTTGAGGCAGACTGATCAAGTGTTTAATATTCCTCACAAGCGCATGTTGAAAGTGGGCATGACTTATGAAATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

756

Amino Acids

85.59

Weight (kDa)

8.18

Isoelectric Point (pI)

36.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	34 - 519	4e-156	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	533 - 755	1.5e-27	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001609)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g30541 FvH4_6g30550 FvH4_c6g00050 FvH4_c6g00050 FvH4_c6g00050
malus_domestica	MD03G1283500.v1.1 MD03G1283600.v1.1 MD10G1230200.v1.1 MD10G1230300.v1.1 MD11G1302600.v1.1 MD11G1302900.v1.1 MD11G1303100.v1.1
prunus_persica	Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1
pyrus_communis	pycom03g22450 pycom03g22460
rosa_chinensis	RchiOBHm_Chr2g0136981 RchiOBHm_Chr5g0020481 RchiOBHm_Chr6g0293251
rosa_laevigata	RLG00000011964 RLG00000033905
rosa_multiflora	Rmu_co8314359.1_g000001 Rmu_sc0001374.1_g000063 Rmu_sc0001374.1_g000064 Rmu_sc0021490.1_g000001 Rmu_ssc0000162.1_g000030
rosa_roxburghii	Rroxscaffold_2G00107800 Rroxscaffold_2G00107830 Rroxscaffold_7G00174310
rosa_rugosa	Rorug02G0336400 Rorug06G0233800 Rorug06G0233800 Rorug06G0233800
rosa_samantha	Rh2AG385800 Rh2BG392700 Rh2CG373000 Rh2DG047100 Rh2DG408800 Rh2DG408900 Rh3DG241600 Rh3DG283500 Rh6AG346700 Rh6BG354100 Rh6CG360600 Rh6DG347100
rosa_wichuraiana	Rw2G003360 Rw2G031570 Rw6G030180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	60
AccII	CGCG	1 cut(s)	1460
AccIII	TCCGGA	1 cut(s)	1195
AciI	CCGC	8 cut(s)	462, 1443, 1467, 1569, 1591, 1640, 1733, 2011
AclI	AACGTT	1 cut(s)	63
AclWI	GGATC	5 cut(s)	378, 743, 1271, 1782, 1952
AcsI	RAATTY	6 cut(s)	385, 1430, 1542, 1582, 2191, 2262
AcuI	CTGAAG	2 cut(s)	781, 882
AcyI	GRCGYC	1 cut(s)	726
AfaI	GTAC	8 cut(s)	142, 260, 499, 897, 1059, 1427, 1520, 1811
AfeI	AGCGCT	1 cut(s)	1745
AfiI	CCNNNNNNNGG	3 cut(s)	640, 1260, 1844
AhdI	GACNNNNNGTC	1 cut(s)	354
AjiI	CACGTC	1 cut(s)	880
AjnI	CCWGG	3 cut(s)	99, 1437, 1597
AloI	GAACNNNNNNTCC	2 cut(s)	443, 475
AluBI	AGCT	9 cut(s)	394, 575, 866, 965, 1085, 1223, 1531, 1558, 2138
AluI	AGCT	9 cut(s)	394, 575, 866, 965, 1085, 1223, 1531, 1558, 2138
Alw21I	GWGCWC	3 cut(s)	396, 923, 1161
Alw26I	GTCTC	1 cut(s)	1705
Alw44I	GTGCAC	2 cut(s)	919, 1157
AlwI	GGATC	5 cut(s)	378, 743, 1271, 1782, 1952
AlwNI	CAGNNNCTG	1 cut(s)	860
Ama87I	CYCGRG	1 cut(s)	395
Aor13HI	TCCGGA	1 cut(s)	1195
Aor51HI	AGCGCT	1 cut(s)	1745
AoxI	GGCC	4 cut(s)	279, 631, 1037, 1600
ApaLI	GTGCAC	2 cut(s)	919, 1157
ApeKI	GCWGC	2 cut(s)	1661, 2126
ApoI	RAATTY	6 cut(s)	385, 1430, 1542, 1582, 2191, 2262
Asp700I	GAANNNNTTC	3 cut(s)	1626, 1784, 1871
AspLEI	GCGC	2 cut(s)	1746, 2235
AspS9I	GGNCC	3 cut(s)	133, 279, 900
AsuHPI	GGTGA	5 cut(s)	100, 138, 229, 1228, 1987
AsuII	TTCGAA	2 cut(s)	1249, 1842
AvaI	CYCGRG	1 cut(s)	395
AvaII	GGWCC	2 cut(s)	133, 900
AxyI	CCTNAGG	2 cut(s)	1240, 1770
BaeGI	GKGCMC	2 cut(s)	923, 1161
BanII	GRGCYC	1 cut(s)	396
BauI	CACGAG	1 cut(s)	1143
BbsI	GAAGAC	3 cut(s)	980, 1355, 2158
Bbv12I	GWGCWC	3 cut(s)	396, 923, 1161
BbvI	GCAGC	2 cut(s)	1648, 2113
BccI	CCATC	5 cut(s)	466, 512, 629, 1616, 1919
BceAI	ACGGC	1 cut(s)	2139
BciT130I	CCWGG	3 cut(s)	101, 1439, 1599
BciVI	GTATCC	1 cut(s)	533
BclI	TGATCA	4 cut(s)	1335, 1534, 2023, 2206
BcoDI	GTCTC	1 cut(s)	1705
BfaI	CTAG	4 cut(s)	68, 1077, 1322, 1736
BfmI	CTRYAG	1 cut(s)	673
BfoI	RGCGCY	1 cut(s)	1747
BfuI	GTATCC	1 cut(s)	533
BisI	GCNGC	5 cut(s)	1467, 1591, 1662, 2012, 2127
BlsI	GCNGC	5 cut(s)	1468, 1592, 1663, 2013, 2128
BmcAI	AGTACT	1 cut(s)	1520
Bme1390I	CCNGG	3 cut(s)	101, 1439, 1599
Bme18I	GGWCC	2 cut(s)	133, 900
BmeRI	GACNNNNNGTC	1 cut(s)	354
BmeT110I	CYCGRG	1 cut(s)	395
BmgBI	CACGTC	1 cut(s)	880
BmgT120I	GGNCC	3 cut(s)	133, 279, 900
BmiI	GGNNCC	3 cut(s)	134, 1731, 1767
BmrFI	CCNGG	3 cut(s)	101, 1439, 1599
BmsI	GCATC	5 cut(s)	177, 192, 434, 805, 1516
BoxI	GACNNNNGTC	1 cut(s)	758
BpiI	GAAGAC	3 cut(s)	980, 1355, 2158
Bpu14I	TTCGAA	2 cut(s)	1249, 1842
BpuEI	CTTGAG	1 cut(s)	1544
Bsa29I	ATCGAT	1 cut(s)	1067
BsaBI	GATNNNNATC	1 cut(s)	1506
BsaHI	GRCGYC	1 cut(s)	726
BsaJI	CCNNGG	3 cut(s)	136, 504, 1438
BsaWI	WCCGGW	2 cut(s)	597, 1195
BsaXI	ACNNNNNCTCC	4 cut(s)	5, 35, 1922, 1952
Bsc4I	CCNNNNNNNGG	3 cut(s)	640, 1260, 1844
Bse1I	ACTGG	2 cut(s)	86, 1970
Bse21I	CCTNAGG	2 cut(s)	1240, 1770
Bse3DI	GCAATG	4 cut(s)	1276, 1676, 1712, 1831
Bse8I	GATNNNNATC	1 cut(s)	1506
BseAI	TCCGGA	1 cut(s)	1195
BseBI	CCWGG	3 cut(s)	101, 1439, 1599
BseCI	ATCGAT	1 cut(s)	1067
BseDI	CCNNGG	3 cut(s)	136, 504, 1438
BseGI	GGATG	5 cut(s)	79, 458, 598, 1313, 1822
BseJI	GATNNNNATC	1 cut(s)	1506
BseLI	CCNNNNNNNGG	3 cut(s)	640, 1260, 1844
BseMI	GCAATG	4 cut(s)	1276, 1676, 1712, 1831
BseMII	CTCAG	1 cut(s)	555
BseNI	ACTGG	2 cut(s)	86, 1970
BseSI	GKGCMC	2 cut(s)	923, 1161
BseXI	GCAGC	2 cut(s)	1648, 2113
Bsh1236I	CGCG	1 cut(s)	1460
BshFI	GGCC	4 cut(s)	281, 633, 1039, 1602
BshVI	ATCGAT	1 cut(s)	1067
BsiHKAI	GWGCWC	3 cut(s)	396, 923, 1161
BsiHKCI	CYCGRG	1 cut(s)	395
BsiSI	CCGG	4 cut(s)	598, 935, 1179, 1196
BslFI	GGGAC	5 cut(s)	146, 313, 897, 1111, 1895
BslI	CCNNNNNNNGG	3 cut(s)	640, 1260, 1844
BsmAI	GTCTC	1 cut(s)	1705
BsmFI	GGGAC	5 cut(s)	146, 313, 897, 1111, 1895
BsnI	GGCC	4 cut(s)	281, 633, 1039, 1602
BsoBI	CYCGRG	1 cut(s)	395
Bsp119I	TTCGAA	2 cut(s)	1249, 1842
Bsp1286I	GDGCHC	3 cut(s)	396, 923, 1161
Bsp13I	TCCGGA	1 cut(s)	1195
Bsp1407I	TGTACA	1 cut(s)	1425
Bsp19I	CCATGG	1 cut(s)	504
BspACI	CCGC	8 cut(s)	462, 1443, 1467, 1569, 1591, 1640, 1733, 2011
BspANI	GGCC	4 cut(s)	281, 633, 1039, 1602
BspCNI	CTCAG	1 cut(s)	554
BspDI	ATCGAT	1 cut(s)	1067
BspEI	TCCGGA	1 cut(s)	1195
BspFNI	CGCG	1 cut(s)	1460
BspHI	TCATGA	2 cut(s)	1332, 1338
BspLI	GGNNCC	3 cut(s)	134, 1731, 1767
BspPI	GGATC	5 cut(s)	378, 743, 1271, 1782, 1952
BspQI	GCTCTTC	1 cut(s)	1734
BspT104I	TTCGAA	2 cut(s)	1249, 1842
BsrDI	GCAATG	4 cut(s)	1276, 1676, 1712, 1831
BsrGI	TGTACA	1 cut(s)	1425
BsrI	ACTGG	2 cut(s)	86, 1970
BssECI	CCNNGG	3 cut(s)	136, 504, 1438
BssNI	GRCGYC	1 cut(s)	726
BssSI	CACGAG	1 cut(s)	1143
BssT1I	CCWWGG	1 cut(s)	504
Bst2BI	CACGAG	1 cut(s)	1143
Bst2UI	CCWGG	3 cut(s)	101, 1439, 1599
Bst4CI	ACNGT	9 cut(s)	677, 757, 895, 900, 986, 1062, 1886, 1919, 1982
Bst6I	CTCTTC	2 cut(s)	1734, 2180
BstACI	GRCGYC	1 cut(s)	726
BstAUI	TGTACA	1 cut(s)	1425
BstBI	TTCGAA	2 cut(s)	1249, 1842
BstC8I	GCNNGC	2 cut(s)	166, 1595
BstDEI	CTNAG	6 cut(s)	541, 844, 911, 1240, 1412, 1770
BstDSI	CCRYGG	1 cut(s)	504
BstF5I	GGATG	5 cut(s)	79, 458, 598, 1313, 1822
BstFNI	CGCG	1 cut(s)	1460
BstH2I	RGCGCY	1 cut(s)	1747
BstHHI	GCGC	2 cut(s)	1746, 2235
BstMAI	GTCTC	1 cut(s)	1705
BstMWI	GCNNNNNNNGC	8 cut(s)	34, 581, 872, 962, 1466, 1599, 1741, 2135
BstNI	CCWGG	3 cut(s)	101, 1439, 1599
BstNSI	RCATGY	2 cut(s)	1597, 2239
BstPAI	GACNNNNGTC	1 cut(s)	758
BstSCI	CCNGG	3 cut(s)	99, 1437, 1597
BstSFI	CTRYAG	1 cut(s)	673
BstSLI	GKGCMC	2 cut(s)	923, 1161
BstUI	CGCG	1 cut(s)	1460
BstV1I	GCAGC	2 cut(s)	1648, 2113
BstV2I	GAAGAC	3 cut(s)	980, 1355, 2158
BstX2I	RGATCY	1 cut(s)	1774
BstYI	RGATCY	1 cut(s)	1774
Bsu15I	ATCGAT	1 cut(s)	1067
Bsu36I	CCTNAGG	2 cut(s)	1240, 1770
BsuI	GTATCC	1 cut(s)	533
BsuRI	GGCC	4 cut(s)	281, 633, 1039, 1602
BsuTUI	ATCGAT	1 cut(s)	1067
BtgI	CCRYGG	1 cut(s)	504
BtgZI	GCGATG	1 cut(s)	531
BtrI	CACGTC	1 cut(s)	880
BtsCI	GGATG	5 cut(s)	79, 458, 598, 1313, 1822
BtsI	GCAGTG	2 cut(s)	1293, 1476
BtsIMutI	CAGTG	3 cut(s)	1293, 1476, 1987
Cac8I	GCNNGC	2 cut(s)	166, 1595
CaiI	CAGNNNCTG	1 cut(s)	860
CciI	TCATGA	2 cut(s)	1332, 1338
CfoI	GCGC	2 cut(s)	1746, 2235
Cfr13I	GGNCC	3 cut(s)	133, 279, 900
ClaI	ATCGAT	1 cut(s)	1067
CseI	GACGC	2 cut(s)	715, 1312
Csp6I	GTAC	8 cut(s)	141, 259, 498, 896, 1058, 1426, 1519, 1810
CspCI	CAANNNNNGTGG	2 cut(s)	475, 510
CviQI	GTAC	8 cut(s)	141, 259, 498, 896, 1058, 1426, 1519, 1810
DdeI	CTNAG	6 cut(s)	541, 844, 911, 1240, 1412, 1770
DriI	GACNNNNNGTC	1 cut(s)	354
Eam1104I	CTCTTC	2 cut(s)	1734, 2180
Eam1105I	GACNNNNNGTC	1 cut(s)	354
EarI	CTCTTC	2 cut(s)	1734, 2180
EciI	GGCGGA	1 cut(s)	1458
Ecl136II	GAGCTC	1 cut(s)	394
Eco130I	CCWWGG	1 cut(s)	504
Eco24I	GRGCYC	1 cut(s)	396
Eco32I	GATATC	3 cut(s)	612, 1448, 1896
Eco47I	GGWCC	2 cut(s)	133, 900
Eco47III	AGCGCT	1 cut(s)	1745
Eco53kI	GAGCTC	1 cut(s)	394
Eco57I	CTGAAG	2 cut(s)	781, 882
Eco81I	CCTNAGG	2 cut(s)	1240, 1770
Eco88I	CYCGRG	1 cut(s)	395
EcoICRI	GAGCTC	1 cut(s)	394
EcoO109I	RGGNCCY	1 cut(s)	133
EcoRII	CCWGG	3 cut(s)	99, 1437, 1597
EcoRV	GATATC	3 cut(s)	612, 1448, 1896
EcoT14I	CCWWGG	1 cut(s)	504
EcoT22I	ATGCAT	1 cut(s)	1276
EcoT38I	GRGCYC	1 cut(s)	396
ErhI	CCWWGG	1 cut(s)	504
FaqI	GGGAC	5 cut(s)	146, 313, 897, 1111, 1895
FauI	CCCGC	1 cut(s)	1647
FauNDI	CATATG	2 cut(s)	1276, 1375
FbaI	TGATCA	4 cut(s)	1335, 1534, 2023, 2206
Fnu4HI	GCNGC	5 cut(s)	1467, 1591, 1662, 2012, 2127
FokI	GGATG	5 cut(s)	86, 445, 605, 1300, 1829
FriOI	GRGCYC	1 cut(s)	396
Fsp4HI	GCNGC	5 cut(s)	1467, 1591, 1662, 2012, 2127
FspBI	CTAG	4 cut(s)	68, 1077, 1322, 1736
GlaI	GCGC	2 cut(s)	1745, 2234
GluI	GCNGC	5 cut(s)	1467, 1591, 1662, 2012, 2127
HaeII	RGCGCY	1 cut(s)	1747
HaeIII	GGCC	4 cut(s)	281, 633, 1039, 1602
HapII	CCGG	4 cut(s)	598, 935, 1179, 1196
HgaI	GACGC	2 cut(s)	715, 1312
HhaI	GCGC	2 cut(s)	1746, 2235
Hin1I	GRCGYC	1 cut(s)	726
Hin6I	GCGC	2 cut(s)	1744, 2233
HinP1I	GCGC	2 cut(s)	1744, 2233
HincII	GTYRAC	1 cut(s)	359
HindII	GTYRAC	1 cut(s)	359
HindIII	AAGCTT	2 cut(s)	864, 963
HinfI	GANTC	6 cut(s)	349, 355, 564, 1233, 1635, 2003
HpaII	CCGG	4 cut(s)	598, 935, 1179, 1196
HphI	GGTGA	5 cut(s)	100, 138, 229, 1228, 1987
Hpy166II	GTNNAC	9 cut(s)	261, 359, 921, 1058, 1159, 1189, 1204, 1812, 1978
Hpy8I	GTNNAC	9 cut(s)	261, 359, 921, 1058, 1159, 1189, 1204, 1812, 1978
Hpy99I	CGWCG	1 cut(s)	757
HpyAV	CCTTC	9 cut(s)	290, 543, 795, 1015, 1220, 1327, 1838, 1849, 2126
HpyCH4III	ACNGT	9 cut(s)	677, 757, 895, 900, 986, 1062, 1886, 1919, 1982
HpyCH4IV	ACGT	3 cut(s)	63, 302, 879
HpyF10VI	GCNNNNNNNGC	8 cut(s)	34, 581, 872, 962, 1466, 1599, 1741, 2135
HpyF3I	CTNAG	6 cut(s)	541, 844, 911, 1240, 1412, 1770
HpySE526I	ACGT	3 cut(s)	63, 302, 879
Hsp92I	GRCGYC	1 cut(s)	726
HspAI	GCGC	2 cut(s)	1744, 2233
Kpn2I	TCCGGA	1 cut(s)	1195
Ksp22I	TGATCA	4 cut(s)	1335, 1534, 2023, 2206
LguI	GCTCTTC	1 cut(s)	1734
LmnI	GCTCC	2 cut(s)	1765, 2135
Lsp1109I	GCAGC	2 cut(s)	1648, 2113
LweI	GCATC	5 cut(s)	177, 192, 434, 805, 1516
MaeI	CTAG	4 cut(s)	68, 1077, 1322, 1736
MaeII	ACGT	3 cut(s)	63, 302, 879
MaeIII	GTNAC	3 cut(s)	88, 1234, 2119
MflI	RGATCY	1 cut(s)	1774
MhlI	GDGCHC	3 cut(s)	396, 923, 1161
MlyI	GAGTC	3 cut(s)	343, 364, 1242
MmeI	TCCRAC	4 cut(s)	776, 1138, 1210, 2028
Mph1103I	ATGCAT	1 cut(s)	1276
MroI	TCCGGA	1 cut(s)	1195
MroXI	GAANNNNTTC	3 cut(s)	1626, 1784, 1871
MseI	TTAA	5 cut(s)	32, 242, 255, 435, 2217
MslI	CAYNNNNRTG	3 cut(s)	195, 1132, 1337
MspI	CCGG	4 cut(s)	598, 935, 1179, 1196
MspR9I	CCNGG	3 cut(s)	101, 1439, 1599
MvaI	CCWGG	3 cut(s)	101, 1439, 1599
MvnI	CGCG	1 cut(s)	1460
MwoI	GCNNNNNNNGC	8 cut(s)	34, 581, 872, 962, 1466, 1599, 1741, 2135
NcoI	CCATGG	1 cut(s)	504
NdeI	CATATG	2 cut(s)	1276, 1375
NlaIV	GGNNCC	3 cut(s)	134, 1731, 1767
NmuCI	GTSAC	3 cut(s)	88, 1234, 2119
NsiI	ATGCAT	1 cut(s)	1276
NspI	RCATGY	2 cut(s)	1597, 2239
NspV	TTCGAA	2 cut(s)	1249, 1842
PaeI	GCATGC	1 cut(s)	1597
PaeR7I	CTCGAG	1 cut(s)	395
PagI	TCATGA	2 cut(s)	1332, 1338
PciSI	GCTCTTC	1 cut(s)	1734
PcsI	WCGNNNNNNNCGW	1 cut(s)	982
PdmI	GAANNNNTTC	3 cut(s)	1626, 1784, 1871
PfeI	GAWTC	3 cut(s)	564, 1635, 2003
PkrI	GCNGC	5 cut(s)	1468, 1592, 1663, 2013, 2128
PleI	GAGTC	3 cut(s)	343, 363, 1241
PpsI	GAGTC	3 cut(s)	343, 363, 1241
PpuMI	RGGWCCY	1 cut(s)	133
PshAI	GACNNNNGTC	1 cut(s)	758
PsiI	TTATAA	1 cut(s)	60
Psp124BI	GAGCTC	1 cut(s)	396
Psp1406I	AACGTT	1 cut(s)	63
Psp5II	RGGWCCY	1 cut(s)	133
Psp6I	CCWGG	3 cut(s)	99, 1437, 1597
PspGI	CCWGG	3 cut(s)	99, 1437, 1597
PspN4I	GGNNCC	3 cut(s)	134, 1731, 1767
PspPI	GGNCC	3 cut(s)	133, 279, 900
PspPPI	RGGWCCY	1 cut(s)	133
PspXI	VCTCGAGB	1 cut(s)	395
PstNI	CAGNNNCTG	1 cut(s)	860
PsuI	RGATCY	1 cut(s)	1774
RsaI	GTAC	8 cut(s)	142, 260, 499, 897, 1059, 1427, 1520, 1811
RsaNI	GTAC	8 cut(s)	141, 259, 498, 896, 1058, 1426, 1519, 1810
RseI	CAYNNNNRTG	3 cut(s)	195, 1132, 1337
SacI	GAGCTC	1 cut(s)	396
SapI	GCTCTTC	1 cut(s)	1734
SaqAI	TTAA	5 cut(s)	32, 242, 255, 435, 2217
SatI	GCNGC	5 cut(s)	1467, 1591, 1662, 2012, 2127
Sau96I	GGNCC	3 cut(s)	133, 279, 900
ScaI	AGTACT	1 cut(s)	1520
SchI	GAGTC	3 cut(s)	343, 364, 1242
ScrFI	CCNGG	3 cut(s)	101, 1439, 1599
SduI	GDGCHC	3 cut(s)	396, 923, 1161
SfaNI	GCATC	5 cut(s)	177, 192, 434, 805, 1516
SfcI	CTRYAG	1 cut(s)	673
Sfr274I	CTCGAG	1 cut(s)	395
SfuI	TTCGAA	2 cut(s)	1249, 1842
SinI	GGWCC	2 cut(s)	133, 900
SlaI	CTCGAG	1 cut(s)	395
SmiMI	CAYNNNNRTG	3 cut(s)	195, 1132, 1337
SmlI	CTYRAG	2 cut(s)	395, 1559
SmoI	CTYRAG	2 cut(s)	395, 1559
SphI	GCATGC	1 cut(s)	1597
SsiI	CCGC	8 cut(s)	462, 1443, 1467, 1569, 1591, 1640, 1733, 2011
SspI	AATATT	1 cut(s)	2221
SspMI	CTAG	4 cut(s)	68, 1077, 1322, 1736
SstI	GAGCTC	1 cut(s)	396
StyD4I	CCNGG	3 cut(s)	99, 1437, 1597
StyI	CCWWGG	1 cut(s)	504
TaaI	ACNGT	9 cut(s)	677, 757, 895, 900, 986, 1062, 1886, 1919, 1982
TaiI	ACGT	3 cut(s)	66, 305, 882
TatI	WGTACW	4 cut(s)	258, 1057, 1425, 1518
TauI	GCSGC	3 cut(s)	1469, 1593, 2014
TfiI	GAWTC	3 cut(s)	564, 1635, 2003
Tru1I	TTAA	5 cut(s)	32, 242, 255, 435, 2217
Tru9I	TTAA	5 cut(s)	32, 242, 255, 435, 2217
TscAI	CASTG	3 cut(s)	1293, 1476, 1987
TseFI	GTSAC	3 cut(s)	88, 1234, 2119
TseI	GCWGC	2 cut(s)	1661, 2126
Tsp45I	GTSAC	3 cut(s)	88, 1234, 2119
TspDTI	ATGAA	7 cut(s)	628, 727, 1848, 1864, 1880, 2087, 2130
TspGWI	ACGGA	3 cut(s)	605, 1675, 1829
TspRI	CASTG	3 cut(s)	1293, 1476, 1987
VneI	GTGCAC	2 cut(s)	919, 1157
VpaK11BI	GGWCC	2 cut(s)	133, 900
XapI	RAATTY	6 cut(s)	385, 1430, 1542, 1582, 2191, 2262
XceI	RCATGY	2 cut(s)	1597, 2239
XhoI	CTCGAG	1 cut(s)	395
XmnI	GAANNNNTTC	3 cut(s)	1626, 1784, 1871
XspI	CTAG	4 cut(s)	68, 1077, 1322, 1736
ZrmI	AGTACT	1 cut(s)	1520
Zsp2I	ATGCAT	1 cut(s)	1276

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.