Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0001609

Physical Location & Seq

Genomic Coordinates Reverse (-)

54905085 .. 54910013

Gene Structure 4929 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG347100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2631 bp

ATGCGTGTGTATGACTGTATAGAAACGAGTACGTGGACTCTTCCTCGTTTGCTTATAAATATCAACATCCTCATCCTCAGTTCTAGGAAATACATCTCCCCCATACTTTTGATTCTCACAACACCCTCAGATTTCCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCAGAGACTCAGAGAGAGATGCCTGAAGATGCAATATTAGAAAACGGTACAGATCAGGATCCTAAAAGCCATGACCGCCAAGAAGAAGTAGTAGTACTTCCTCATCATCCGGATCATCAACAACTCAAAGGCAAGAAACTTTCATGGCAAAAGTTACGTAGATTCGACTCCTTGGATATCGAGTCACGCAGTGTCAACGGTCACCATGGCCATAGCTCCCAGGGTGCAAGTTGGTCGGTGATACTGCACCTAGCATTTCAGAGCCTTGGAATAGTCTATGGAGACATTGGTACATCACCTCTATATGTGTATGCGAGTACCTTCACCAATGGCATCAAGGACATAGATGACATCTTGGGTGTTCTTTCTTTGATCATATACACCATCACCCTAATCCCCTTGATTAAGTACGTCTTCATCGTCCTCCGAGCCAACGATAATGGCGACGGAGGGACGTTCGCACTTTACTCTCTGTTGTGTCGATATGCCAAGGTTGGTTTGACTCCTAGTCAGCAAGCCGAAGACCGCGATGTTTCAAATTTCCAACTTGAGTTGCCTAGCAAACGCCTAAGGAGGGCATCGAAGCTTAAATCAAAATTAGAGAACAGCAGATTTGCCAAGTTCTTTTTACTATTCGCCACCATGCTTGGTACTTCTATGGTTATTGGTGATGGCGTCCTCACTCCTTGCATCTCAGTTCTATCGGCTGTTGGAGGGATGAAACAAGCTACATCTGCCCTGACCGACGATATGGTTGTTTGGATATCAGTAGGCATCTTGGTTTGCCTATTCATGGTTCAGAGATTTGGAACTGACAAAGTTGGCTACAGTTTTGCACCAATAATTTGTGTTTGGTTTACCTTCATTGGTGGCATTGGCGTCTACAATTTCTTCAAATACGATCCAACAGTTTTGAAAGCTCTAAATCCATTATACATAGTCGATTACTTCAGGAGAAACAAAGGAGATGCTTGGATTTCCCTCGGTGGCATTGTTCTTGCCATTACAGGAACTGAAGCCCTGTTTGCCGATGTTGGCCACTTCACAGTTCGATCCATTCAGATTAGTATGTGCTCAGTGACTTACCCAGCTCTCCTACTTGCATATACCGGACAAGCCTCTTTTCTGCGTGAGCACAAGAATCTAGTTTCGAACACCTTCTATGAGTCCATACCAAATCCTTTGTATTGGCCTATGTTTGGGGTTGCCGTAATGGCAGCAATCATAGCCAGCCAAGCCATGATTTCGGGTACCTTCTCAATCATCCAACAGTCTCTTTCACTAGGGTGTTTCCCTCGTGTGAAAATTGTGCACACATCGACCAAGTATGAAGGACAAGTTTACATTCCGGAGGTCAACTACCTTCTCATGCTGGCTTGTGTTGGAGTCACCTTGGGTTTCAGAAGCACCACAAAGATTGGAAATGCATACGGCATAGCAGTGGTATTTGTAATGACGCTTACGTCGTCCTTCCTGGTGCTAATCATGATTATGATTTGGAAAACCCCAATACTTCTCATTATCTCATATGTTCTTATCATCGGAAGCGTGGAGCTTATGTACTTAAGTTCGGTGCTCTACAAATTTGACCAAGGTGGATATCTTCCTCTCGCATTTGCCACTTTACTGATGCTCATAATGTTTGTGTGGAACGATGTTCATCGAAGAAAGTACTACTACGAGCTCGAGCACAAAATTTCCCCTGAAAAGCTCAAGCAACTTGCCGTGGACAAAAACTTTTGCCGCATGCCTGGCCTCGCCATGTTCTATTCAGAACTCGTTCAAGGAATTCCTCCAATCTTCAATCATTACGCGGCAAATGTGCCTGCTTTGCACTCAGTCCTGGTTTTTGTCTCCATCAAATCATTACCGATAAGCAAGGTTCCGTTAGAGGAGCGTTTTCTTTTCCGCAGAGTGGAGCCTAGAGAGCTTAATGTGTTCCGATGTGTCGCTAGATACGGGTACACAGATGTCCGAAATGAGAATGAACCCTTTGAAGGATTGCTGATAGAAAAATTGAAGGAGTTCATAAGGGATGACTTTTGGTTATCTCAAAGAACCATGCACATAACCGATGGAGAGAAATTGGAAACGGAAGTTTCTGCTGAGTTAGAAGGTGATGATCATGAGTTGGTTAATGGAAATGGTCATAATGAGAATGAGAACGAAAACGAGAATGTAAAGCAAGTACTTGATGATGATGAAGATCAAAAGAAACAACAAGACGATGTACTGTTGGAGACAGAGATTGAGGCGATTGACAAAGCGTGGCATCGGGGGGTTGTGCACTTAATTGGTGAGAATGAAGTGACGGCTGCGAAAGGAGCTGGGATAGCGAAAAGGATCTTGATTGATTATGCTTACAATTTCTTGAAGAGAAATCTGAGGCAGAGTGACAAAGTGTTTGATATTCCTCACAAGCGCATGTTAAAAGTGGGCATGACTTATGAACTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

876

Amino Acids

98.85

Weight (kDa)

6.34

Isoelectric Point (pI)

35.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	142 - 628	2.9e-168	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	642 - 876	2.4e-26	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001609)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g30541 FvH4_6g30550 FvH4_c6g00050 FvH4_c6g00050 FvH4_c6g00050
malus_domestica	MD03G1283500.v1.1 MD03G1283600.v1.1 MD10G1230200.v1.1 MD10G1230300.v1.1 MD11G1302600.v1.1 MD11G1302900.v1.1 MD11G1303100.v1.1
prunus_persica	Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1
pyrus_communis	pycom03g22450 pycom03g22460
rosa_chinensis	RchiOBHm_Chr2g0136981 RchiOBHm_Chr5g0020481 RchiOBHm_Chr6g0293251
rosa_laevigata	RLG00000011964 RLG00000033905
rosa_multiflora	Rmu_co8314359.1_g000001 Rmu_sc0001374.1_g000063 Rmu_sc0001374.1_g000064 Rmu_sc0021490.1_g000001 Rmu_ssc0000162.1_g000030
rosa_roxburghii	Rroxscaffold_2G00107800 Rroxscaffold_2G00107830 Rroxscaffold_7G00174310
rosa_rugosa	Rorug02G0336400 Rorug06G0233800 Rorug06G0233800 Rorug06G0233800
rosa_samantha	Rh2AG385800 Rh2BG392700 Rh2CG373000 Rh2DG047100 Rh2DG408800 Rh2DG408900 Rh3DG241600 Rh3DG283500 Rh6AG346700 Rh6BG354100 Rh6CG360600 Rh6DG347100
rosa_wichuraiana	Rw2G003360 Rw2G031570 Rw6G030180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	56
AasI	GACNNNNNNGTC	1 cut(s)	1636
Acc65I	GGTACC	1 cut(s)	1424
AccB1I	GGYRCC	1 cut(s)	1424
AccI	GTMKAC	1 cut(s)	1056
AccII	CGCG	2 cut(s)	702, 1988
AccIII	TCCGGA	2 cut(s)	283, 1522
AciI	CCGC	5 cut(s)	250, 700, 1918, 1988, 2083
AclWI	GGATC	6 cut(s)	227, 240, 294, 1070, 1221, 2525
AcoI	YGGCCR	2 cut(s)	382, 1210
AcsI	RAATTY	4 cut(s)	712, 1757, 1869, 1962
AcuI	CTGAAG	3 cut(s)	219, 1108, 1209
AcyI	GRCGYC	2 cut(s)	849, 1053
AdeI	CACNNNGTG	1 cut(s)	365
AfiI	CCNNNNNNNGG	5 cut(s)	748, 967, 1373, 2089, 2171
AflII	CTTAAG	1 cut(s)	1738
AgsI	TTSAA	8 cut(s)	711, 1069, 1090, 1958, 1978, 2171, 2194, 2548
AhdI	GACNNNNNGTC	1 cut(s)	681
AjnI	CCWGG	4 cut(s)	393, 1647, 1924, 2016
AleI	CACNNNNGTG	1 cut(s)	1459
Alw21I	GWGCWC	7 cut(s)	1250, 1311, 1488, 1752, 1860, 1866, 2463
Alw26I	GTCTC	5 cut(s)	173, 450, 1452, 2032, 2408
Alw44I	GTGCAC	2 cut(s)	1484, 2459
AlwI	GGATC	6 cut(s)	227, 240, 294, 1070, 1221, 2525
AlwNI	CAGNNNCTG	1 cut(s)	1187
Ama87I	CYCGRG	1 cut(s)	1859
Aor13HI	TCCGGA	2 cut(s)	283, 1522
AoxI	GGCC	4 cut(s)	382, 1210, 1364, 1927
ApaLI	GTGCAC	2 cut(s)	1484, 2459
ApeKI	GCWGC	2 cut(s)	1391, 2489
ApoI	RAATTY	4 cut(s)	712, 1757, 1869, 1962
ArsI	GACNNNNNNTTYG	2 cut(s)	2561, 2593
Asp700I	GAANNNNTTC	3 cut(s)	1331, 1953, 2198
Asp718I	GGTACC	1 cut(s)	1424
AspLEI	GCGC	1 cut(s)	2598
AsuHPI	GGTGA	9 cut(s)	368, 424, 462, 490, 553, 854, 1555, 2303, 2483
AsuII	TTCGAA	1 cut(s)	1325
AvaI	CYCGRG	1 cut(s)	1859
AxyI	CCTNAGG	1 cut(s)	743
BaeGI	GKGCMC	2 cut(s)	1488, 2463
BalI	TGGCCA	2 cut(s)	384, 1212
BamHI	GGATCC	1 cut(s)	232
BanI	GGYRCC	1 cut(s)	1424
BanII	GRGCYC	1 cut(s)	1860
BarI	GAAGNNNNNNTAC	4 cut(s)	252, 255, 284, 287
BauI	CACGAG	1 cut(s)	1470
BbsI	GAAGAC	2 cut(s)	580, 702
Bbv12I	GWGCWC	7 cut(s)	1250, 1311, 1488, 1752, 1860, 1866, 2463
BbvI	GCAGC	2 cut(s)	1403, 2476
BccI	CCATC	4 cut(s)	566, 839, 2039, 2243
BceAI	ACGGC	4 cut(s)	1367, 1621, 1883, 2502
BcgI	CGANNNNNNTGC	2 cut(s)	390, 424
BciT130I	CCWGG	4 cut(s)	395, 1649, 1926, 2018
BclI	TGATCA	2 cut(s)	546, 2296
BcoDI	GTCTC	5 cut(s)	173, 450, 1452, 2032, 2408
BfaI	CTAG	8 cut(s)	84, 425, 681, 732, 1319, 1457, 2097, 2127
BfmI	CTRYAG	2 cut(s)	1000, 2627
BfrI	CTTAAG	1 cut(s)	1738
BglI	GCCNNNNNGGC	1 cut(s)	1388
BisI	GCNGC	4 cut(s)	1392, 1918, 1989, 2490
BlsI	GCNGC	4 cut(s)	1393, 1919, 1990, 2491
BmcAI	AGTACT	3 cut(s)	270, 1847, 2364
Bme1390I	CCNGG	4 cut(s)	395, 1649, 1926, 2018
BmeRI	GACNNNNNGTC	1 cut(s)	681
BmeT110I	CYCGRG	1 cut(s)	1859
BmiI	GGNNCC	4 cut(s)	234, 1426, 2058, 2094
BmrFI	CCNGG	4 cut(s)	395, 1649, 1926, 2018
BmsI	GCATC	9 cut(s)	183, 193, 516, 761, 873, 957, 1132, 1794, 2455
BpiI	GAAGAC	2 cut(s)	580, 702
Bpu14I	TTCGAA	1 cut(s)	1325
BpuEI	CTTGAG	2 cut(s)	743, 1871
BsaAI	YACGTR	2 cut(s)	33, 332
BsaBI	GATNNNNATC	3 cut(s)	231, 1833, 2379
BsaHI	GRCGYC	2 cut(s)	849, 1053
BsaWI	WCCGGW	3 cut(s)	283, 1283, 1522
BsaXI	ACNNNNNCTCC	2 cut(s)	1718, 1748
Bsc4I	CCNNNNNNNGG	5 cut(s)	748, 967, 1373, 2089, 2171
Bse21I	CCTNAGG	1 cut(s)	743
Bse8I	GATNNNNATC	3 cut(s)	231, 1833, 2379
BseAI	TCCGGA	2 cut(s)	283, 1522
BseBI	CCWGG	4 cut(s)	395, 1649, 1926, 2018
BseGI	GGATG	6 cut(s)	66, 72, 280, 897, 1437, 2215
BseJI	GATNNNNATC	3 cut(s)	231, 1833, 2379
BseLI	CCNNNNNNNGG	5 cut(s)	748, 967, 1373, 2089, 2171
BseMII	CTCAG	9 cut(s)	91, 141, 189, 197, 882, 1263, 2025, 2271, 2549
BseRI	GAGGAG	1 cut(s)	2081
BseSI	GKGCMC	2 cut(s)	1488, 2463
BseXI	GCAGC	2 cut(s)	1403, 2476
BseYI	CCCAGC	2 cut(s)	1261, 2501
BsgI	GTGCAG	1 cut(s)	404
Bsh1236I	CGCG	2 cut(s)	702, 1988
BshFI	GGCC	4 cut(s)	384, 1212, 1366, 1929
BshNI	GGYRCC	1 cut(s)	1424
BsiHKAI	GWGCWC	7 cut(s)	1250, 1311, 1488, 1752, 1860, 1866, 2463
BsiHKCI	CYCGRG	1 cut(s)	1859
BsiSI	CCGG	3 cut(s)	284, 1284, 1523
BslFI	GGGAC	1 cut(s)	640
BslI	CCNNNNNNNGG	5 cut(s)	748, 967, 1373, 2089, 2171
BsmAI	GTCTC	5 cut(s)	173, 450, 1452, 2032, 2408
BsmFI	GGGAC	1 cut(s)	640
BsnI	GGCC	4 cut(s)	384, 1212, 1366, 1929
BsoBI	CYCGRG	1 cut(s)	1859
Bsp119I	TTCGAA	1 cut(s)	1325
Bsp1286I	GDGCHC	7 cut(s)	1250, 1311, 1488, 1752, 1860, 1866, 2463
Bsp13I	TCCGGA	2 cut(s)	283, 1522
Bsp143I	GATC	9 cut(s)	226, 232, 286, 546, 1075, 1226, 2296, 2380, 2517
Bsp19I	CCATGG	1 cut(s)	379
BspACI	CCGC	5 cut(s)	250, 700, 1918, 1988, 2083
BspANI	GGCC	4 cut(s)	384, 1212, 1366, 1929
BspCNI	CTCAG	9 cut(s)	90, 140, 188, 196, 881, 1262, 2024, 2272, 2550
BspEI	TCCGGA	2 cut(s)	283, 1522
BspFNI	CGCG	2 cut(s)	702, 1988
BspHI	TCATGA	2 cut(s)	1659, 2299
BspLI	GGNNCC	4 cut(s)	234, 1426, 2058, 2094
BspPI	GGATC	6 cut(s)	227, 240, 294, 1070, 1221, 2525
BspT104I	TTCGAA	1 cut(s)	1325
BspT107I	GGYRCC	1 cut(s)	1424
BspTI	CTTAAG	1 cut(s)	1738
BssMI	GATC	9 cut(s)	226, 232, 286, 546, 1075, 1226, 2296, 2380, 2517
BssNI	GRCGYC	2 cut(s)	849, 1053
BssSI	CACGAG	1 cut(s)	1470
BssT1I	CCWWGG	6 cut(s)	345, 379, 439, 663, 1566, 1765
Bst2BI	CACGAG	1 cut(s)	1470
Bst2UI	CCWGG	4 cut(s)	395, 1649, 1926, 2018
Bst4CI	ACNGT	8 cut(s)	17, 221, 374, 1004, 1084, 1222, 1446, 2409
Bst6I	CTCTTC	2 cut(s)	45, 2543
BstACI	GRCGYC	2 cut(s)	849, 1053
BstAFI	CTTAAG	1 cut(s)	1738
BstBAI	YACGTR	2 cut(s)	33, 332
BstBI	TTCGAA	1 cut(s)	1325
BstC8I	GCNNGC	5 cut(s)	690, 1405, 1548, 1922, 2001
BstDSI	CCRYGG	2 cut(s)	379, 1899
BstEII	GGTNACC	1 cut(s)	374
BstF5I	GGATG	6 cut(s)	66, 72, 280, 897, 1437, 2215
BstFNI	CGCG	2 cut(s)	702, 1988
BstHHI	GCGC	1 cut(s)	2598
BstKTI	GATC	9 cut(s)	229, 235, 289, 549, 1078, 1229, 2299, 2383, 2520
BstMAI	GTCTC	5 cut(s)	173, 450, 1452, 2032, 2408
BstMBI	GATC	9 cut(s)	226, 232, 286, 546, 1075, 1226, 2296, 2380, 2517
BstNI	CCWGG	4 cut(s)	395, 1649, 1926, 2018
BstNSI	RCATGY	2 cut(s)	1924, 2602
BstPI	GGTNACC	1 cut(s)	374
BstSCI	CCNGG	4 cut(s)	393, 1647, 1924, 2016
BstSFI	CTRYAG	2 cut(s)	1000, 2627
BstSLI	GKGCMC	2 cut(s)	1488, 2463
BstSNI	TACGTA	1 cut(s)	332
BstUI	CGCG	2 cut(s)	702, 1988
BstV1I	GCAGC	2 cut(s)	1403, 2476
BstV2I	GAAGAC	2 cut(s)	580, 702
BstX2I	RGATCY	2 cut(s)	232, 2517
BstYI	RGATCY	2 cut(s)	232, 2517
Bsu36I	CCTNAGG	1 cut(s)	743
BsuRI	GGCC	4 cut(s)	384, 1212, 1366, 1929
BtgI	CCRYGG	2 cut(s)	379, 1899
BtgZI	GCGATG	1 cut(s)	717
BtsCI	GGATG	6 cut(s)	66, 72, 280, 897, 1437, 2215
BtsI	GCAGTG	2 cut(s)	370, 1620
BtsIMutI	CAGTG	3 cut(s)	370, 1257, 1620
Cac8I	GCNNGC	5 cut(s)	690, 1405, 1548, 1922, 2001
CaiI	CAGNNNCTG	1 cut(s)	1187
CciI	TCATGA	2 cut(s)	1659, 2299
CfoI	GCGC	1 cut(s)	2598
CseI	GACGC	3 cut(s)	838, 1042, 1639
CspCI	CAANNNNNGTGG	4 cut(s)	802, 837, 1573, 1608
DpnI	GATC	9 cut(s)	228, 234, 288, 548, 1077, 1228, 2298, 2382, 2519
DpnII	GATC	9 cut(s)	226, 232, 286, 546, 1075, 1226, 2296, 2380, 2517
DraIII	CACNNNGTG	1 cut(s)	365
DrdI	GACNNNNNNGTC	1 cut(s)	1636
DriI	GACNNNNNGTC	1 cut(s)	681
DseDI	GACNNNNNNGTC	1 cut(s)	1636
EaeI	YGGCCR	2 cut(s)	382, 1210
Eam1104I	CTCTTC	2 cut(s)	45, 2543
Eam1105I	GACNNNNNGTC	1 cut(s)	681
EarI	CTCTTC	2 cut(s)	45, 2543
Ecl136II	GAGCTC	1 cut(s)	1858
Eco105I	TACGTA	1 cut(s)	332
Eco130I	CCWWGG	6 cut(s)	345, 379, 439, 663, 1566, 1765
Eco24I	GRGCYC	1 cut(s)	1860
Eco32I	GATATC	3 cut(s)	352, 939, 1775
Eco53kI	GAGCTC	1 cut(s)	1858
Eco57I	CTGAAG	3 cut(s)	219, 1108, 1209
Eco81I	CCTNAGG	1 cut(s)	743
Eco88I	CYCGRG	1 cut(s)	1859
Eco91I	GGTNACC	1 cut(s)	374
EcoICRI	GAGCTC	1 cut(s)	1858
EcoO65I	GGTNACC	1 cut(s)	374
EcoRI	GAATTC	1 cut(s)	1962
EcoRII	CCWGG	4 cut(s)	393, 1647, 1924, 2016
EcoRV	GATATC	3 cut(s)	352, 939, 1775
EcoT14I	CCWWGG	6 cut(s)	345, 379, 439, 663, 1566, 1765
EcoT22I	ATGCAT	1 cut(s)	1603
EcoT38I	GRGCYC	1 cut(s)	1860
ErhI	CCWWGG	6 cut(s)	345, 379, 439, 663, 1566, 1765
FalI	AAGNNNNNCTT	2 cut(s)	572, 604
FaqI	GGGAC	1 cut(s)	640
FauNDI	CATATG	1 cut(s)	1702
FbaI	TGATCA	2 cut(s)	546, 2296
FblI	GTMKAC	1 cut(s)	1056
Fnu4HI	GCNGC	4 cut(s)	1392, 1918, 1989, 2490
FokI	GGATG	6 cut(s)	53, 59, 267, 904, 1424, 2222
FriOI	GRGCYC	1 cut(s)	1860
Fsp4HI	GCNGC	4 cut(s)	1392, 1918, 1989, 2490
FspBI	CTAG	8 cut(s)	84, 425, 681, 732, 1319, 1457, 2097, 2127
GlaI	GCGC	1 cut(s)	2597
GluI	GCNGC	4 cut(s)	1392, 1918, 1989, 2490
GsaI	CCCAGC	2 cut(s)	1265, 2505
HaeIII	GGCC	4 cut(s)	384, 1212, 1366, 1929
HapII	CCGG	3 cut(s)	284, 1284, 1523
HgaI	GACGC	3 cut(s)	838, 1042, 1639
HhaI	GCGC	1 cut(s)	2598
Hin1I	GRCGYC	2 cut(s)	849, 1053
Hin6I	GCGC	1 cut(s)	2596
HinP1I	GCGC	1 cut(s)	2596
HincII	GTYRAC	2 cut(s)	370, 1531
HindII	GTYRAC	2 cut(s)	370, 1531
HindIII	AAGCTT	1 cut(s)	758
HpaII	CCGG	3 cut(s)	284, 1284, 1523
HphI	GGTGA	9 cut(s)	368, 424, 462, 490, 553, 854, 1555, 2303, 2483
Hpy188III	TCNNGA	8 cut(s)	230, 284, 1126, 1523, 1660, 2300, 2521, 2545
Hpy99I	CGWCG	3 cut(s)	623, 923, 1642
HpyCH4III	ACNGT	8 cut(s)	17, 221, 374, 1004, 1084, 1222, 1446, 2409
HpyCH4IV	ACGT	5 cut(s)	32, 331, 585, 629, 1637
HpySE526I	ACGT	5 cut(s)	32, 331, 585, 629, 1637
Hsp92I	GRCGYC	2 cut(s)	849, 1053
HspAI	GCGC	1 cut(s)	2596
Kpn2I	TCCGGA	2 cut(s)	283, 1522
KpnI	GGTACC	1 cut(s)	1428
Ksp22I	TGATCA	2 cut(s)	546, 2296
Kzo9I	GATC	9 cut(s)	226, 232, 286, 546, 1075, 1226, 2296, 2380, 2517
LmnI	GCTCC	5 cut(s)	395, 1726, 2068, 2092, 2498
Lsp1109I	GCAGC	2 cut(s)	1403, 2476
LweI	GCATC	9 cut(s)	183, 193, 516, 761, 873, 957, 1132, 1794, 2455
MaeI	CTAG	8 cut(s)	84, 425, 681, 732, 1319, 1457, 2097, 2127
MaeII	ACGT	5 cut(s)	32, 331, 585, 629, 1637
MaeIII	GTNAC	7 cut(s)	327, 357, 374, 1252, 1561, 2482, 2567
MalI	GATC	9 cut(s)	228, 234, 288, 548, 1077, 1228, 2298, 2382, 2519
MboI	GATC	9 cut(s)	226, 232, 286, 546, 1075, 1226, 2296, 2380, 2517
MflI	RGATCY	2 cut(s)	232, 2517
MhlI	GDGCHC	7 cut(s)	1250, 1311, 1488, 1752, 1860, 1866, 2463
MlsI	TGGCCA	2 cut(s)	384, 1212
MluNI	TGGCCA	2 cut(s)	384, 1212
MlyI	GAGTC	7 cut(s)	31, 175, 335, 365, 670, 1349, 1569
MmeI	TCCRAC	6 cut(s)	742, 865, 1103, 1465, 1537, 2391
Mox20I	TGGCCA	2 cut(s)	384, 1212
Mph1103I	ATGCAT	1 cut(s)	1603
MroI	TCCGGA	2 cut(s)	283, 1522
MroXI	GAANNNNTTC	3 cut(s)	1331, 1953, 2198
MscI	TGGCCA	2 cut(s)	384, 1212
MseI	TTAA	7 cut(s)	579, 762, 1739, 2106, 2310, 2465, 2603
MslI	CAYNNNNRTG	3 cut(s)	1459, 1613, 1664
Msp20I	TGGCCA	2 cut(s)	384, 1212
MspCI	CTTAAG	1 cut(s)	1738
MspI	CCGG	3 cut(s)	284, 1284, 1523
MspR9I	CCNGG	4 cut(s)	395, 1649, 1926, 2018
MvaI	CCWGG	4 cut(s)	395, 1649, 1926, 2018
MvnI	CGCG	2 cut(s)	702, 1988
NcoI	CCATGG	1 cut(s)	379
NdeI	CATATG	1 cut(s)	1702
NdeII	GATC	9 cut(s)	226, 232, 286, 546, 1075, 1226, 2296, 2380, 2517
NlaIV	GGNNCC	4 cut(s)	234, 1426, 2058, 2094
NmuCI	GTSAC	6 cut(s)	357, 374, 1252, 1561, 2482, 2567
NsiI	ATGCAT	1 cut(s)	1603
NspI	RCATGY	2 cut(s)	1924, 2602
NspV	TTCGAA	1 cut(s)	1325
OliI	CACNNNNGTG	1 cut(s)	1459
PaeI	GCATGC	1 cut(s)	1924
PaeR7I	CTCGAG	1 cut(s)	1859
PagI	TCATGA	2 cut(s)	1659, 2299
PasI	CCCWGGG	1 cut(s)	394
PcsI	WCGNNNNNNNCGW	3 cut(s)	591, 615, 1637
PdmI	GAANNNNTTC	3 cut(s)	1331, 1953, 2198
PfeI	GAWTC	3 cut(s)	112, 336, 1315
PkrI	GCNGC	4 cut(s)	1393, 1919, 1990, 2491
PleI	GAGTC	7 cut(s)	31, 175, 335, 364, 670, 1348, 1568
PpsI	GAGTC	7 cut(s)	31, 175, 335, 364, 670, 1348, 1568
Ppu21I	YACGTR	2 cut(s)	33, 332
PsiI	TTATAA	1 cut(s)	56
Psp124BI	GAGCTC	1 cut(s)	1860
Psp6I	CCWGG	4 cut(s)	393, 1647, 1924, 2016
PspEI	GGTNACC	1 cut(s)	374
PspFI	CCCAGC	2 cut(s)	1261, 2501
PspGI	CCWGG	4 cut(s)	393, 1647, 1924, 2016
PspN4I	GGNNCC	4 cut(s)	234, 1426, 2058, 2094
PspXI	VCTCGAGB	1 cut(s)	1859
PstNI	CAGNNNCTG	1 cut(s)	1187
PsuI	RGATCY	2 cut(s)	232, 2517
RseI	CAYNNNNRTG	3 cut(s)	1459, 1613, 1664
SacI	GAGCTC	1 cut(s)	1860
SaqAI	TTAA	7 cut(s)	579, 762, 1739, 2106, 2310, 2465, 2603
SatI	GCNGC	4 cut(s)	1392, 1918, 1989, 2490
Sau3AI	GATC	9 cut(s)	226, 232, 286, 546, 1075, 1226, 2296, 2380, 2517
ScaI	AGTACT	3 cut(s)	270, 1847, 2364
SchI	GAGTC	7 cut(s)	31, 175, 335, 365, 670, 1349, 1569
ScrFI	CCNGG	4 cut(s)	395, 1649, 1926, 2018
SduI	GDGCHC	7 cut(s)	1250, 1311, 1488, 1752, 1860, 1866, 2463
SfaNI	GCATC	9 cut(s)	183, 193, 516, 761, 873, 957, 1132, 1794, 2455
SfcI	CTRYAG	2 cut(s)	1000, 2627
Sfr274I	CTCGAG	1 cut(s)	1859
SfuI	TTCGAA	1 cut(s)	1325
SlaI	CTCGAG	1 cut(s)	1859
SmiMI	CAYNNNNRTG	3 cut(s)	1459, 1613, 1664
SmlI	CTYRAG	4 cut(s)	722, 1738, 1859, 1886
SmoI	CTYRAG	4 cut(s)	722, 1738, 1859, 1886
SnaBI	TACGTA	1 cut(s)	332
SphI	GCATGC	1 cut(s)	1924
SsiI	CCGC	5 cut(s)	250, 700, 1918, 1988, 2083
SspI	AATATT	1 cut(s)	210
SspMI	CTAG	8 cut(s)	84, 425, 681, 732, 1319, 1457, 2097, 2127
SstI	GAGCTC	1 cut(s)	1860
StyD4I	CCNGG	4 cut(s)	393, 1647, 1924, 2016
StyI	CCWWGG	6 cut(s)	345, 379, 439, 663, 1566, 1765
TaaI	ACNGT	8 cut(s)	17, 221, 374, 1004, 1084, 1222, 1446, 2409
TaiI	ACGT	5 cut(s)	35, 334, 588, 632, 1640
TaqII	GACCGA	1 cut(s)	932
TatI	WGTACW	5 cut(s)	268, 1734, 1845, 2362, 2404
TauI	GCSGC	2 cut(s)	1920, 1991
TfiI	GAWTC	3 cut(s)	112, 336, 1315
Tru1I	TTAA	7 cut(s)	579, 762, 1739, 2106, 2310, 2465, 2603
Tru9I	TTAA	7 cut(s)	579, 762, 1739, 2106, 2310, 2465, 2603
TscAI	CASTG	3 cut(s)	370, 1257, 1620
TseFI	GTSAC	6 cut(s)	357, 374, 1252, 1561, 2482, 2567
TseI	GCWGC	2 cut(s)	1391, 2489
Tsp45I	GTSAC	6 cut(s)	357, 374, 1252, 1561, 2482, 2567
TspGWI	ACGGA	3 cut(s)	636, 2049, 2282
TspRI	CASTG	3 cut(s)	370, 1257, 1620
Vha464I	CTTAAG	1 cut(s)	1738
VneI	GTGCAC	2 cut(s)	1484, 2459
XapI	RAATTY	4 cut(s)	712, 1757, 1869, 1962
XceI	RCATGY	2 cut(s)	1924, 2602
XhoI	CTCGAG	1 cut(s)	1859
XmiI	GTMKAC	1 cut(s)	1056
XmnI	GAANNNNTTC	3 cut(s)	1331, 1953, 2198
XspI	CTAG	8 cut(s)	84, 425, 681, 732, 1319, 1457, 2097, 2127
ZrmI	AGTACT	3 cut(s)	270, 1847, 2364
Zsp2I	ATGCAT	1 cut(s)	1603

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.