Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0001609

Physical Location & Seq

Genomic Coordinates Reverse (-)

55988354 .. 55992860

Gene Structure 4507 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ26313

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2439 bp

ATGCCTGAAGATGCAATATTAGAAAACGGTACAGATCAGGATCCTAAAAGCCATCACCGCCAAGAAGAAGTAGTAGTACTTCCTCATCATCCGGATCATCAACAACTCAAAGGCAAGAAACTTTCATGGCAAAAGTTACGTAGATTCGACTCCTTGGACATCGAGTCACGCAGTGTCAACGGTCACCATGGCCATAGCTCCCAGGGTGCAAGTTGGTCGGTGATACTGCACCTAGCATTTCAGAGCCTTGGAATAGTCTATGGAGACATTGGTACATCACCTCTATATGTGTATGCGAGTACCTTCACCAATGGCATCAAGGACACAGATGACATCTTGGGTGTTCTTTCTTTGATCATATACACCCTCACCTTAATCCCCTTGATTAAGTACGTCTTCATCGTCCTCCGAGCCAACGATAATGGCGACGGAGGGACGTTCGCACTTTACTCTCTGTTGTGTCGATATGCCAAGGTTGGTTTGACTCCGAGTCAGCAAGCCGAAGACCGCGATGTTTCAAATTTCCAACTTGAGTTGCCTAGCAAACGCCTAAGGAGGGCATCGAAGCTTAAATCAAAATTAGAGAACAGCAGATTTGCCAAGTACTTTTTACTATTCGCCACCATGCTTGGTACTTCTATGGTTATTGGTGATGGCGTCCTCACTCCTTGCATCTCAGTTCTATCGGCTGTTGGAGGGATGAAACAAGCTACATCTGCCCTGACCGACGATATGGTTGTTTGGATATCAGTAGGCATCTTGGTTTGCCTATTCATGGTTCAGAGATTTGGAACTGACAAAGTTGGCTACAGTTTTGCACCAATAATTTGTGTTTGGTTTACCTTCATTGGTGGCATTGGCGTCTACAATTTCTTCAAATACGATCCAACAGTTTTGAAAGCTCTAAATCCACGATACATAGTCGATTACTTCAGGAGAAACAAAGAAGATGCTTGGATTTCCCTCGGTGGCATTGTTCTTGCCATTACAGGAACTGAAGCCCTGTTTGCCGATGTTGGCCACTTCACAGTTCGATCCATTCAGATTAGTATGTGCTCAGTGACTTACCCAGCTCTCCTACTTGCATATACCGGACAAGCCTCTTTTCTGCGTGAGCACAAGACTCTAGTTTCGAACACCTTCTATGAGTCCATACCAAATCCTTTGTATTGGCCTATGTTTGGGGTTGCCGTAATGGCATCAATCATAGCCAGCCAAGCCATGATTTCGGGTACCTTCTCAATCATCCAACAGTCTCTTTCACTAGGGTGTTTCCCTCGTGTGAAAATTGTGCACACATCGACCAAGTATGAAGGACAAGTTTACATTCCGGAGGTCAACTACCTTCTCATGCTGGCTTGTGTTGGAGTCACCTTGGGTTTCAGAAGCACCACAAAAATTGGAAATGCATACGGCATAGCAGTGGTATTTGTAATGACGCTTACGTCGTCCTTCCTGGTGCTAATCATGATTATGATTTGGAAAACCCCAATACTTCTCATTATCTCATATGTTCTTATCATCGGAAGCGTGGAGCTTATGTACTTGAGTTCGGTGCTCTACAAATTTGACCAAGGTGGATATCTTCCTTTGGCATTTGCCACTTTACTGATGCTCATAATGTTTGTGTGGAACGATGTTCATCGAAGAAAGTACTACTACGAGCTCGAGCACAAAATTTCCCCTGAAAAGCTCAAGCAACTTGCCGTGGACAAAAACTTTTGCCGCATGCCTGGCCTCGCCATGTTCTATTCAGAACTCGTTCAAGGAATTCCTCCAATCTTCAGTCATTACGCGGCAAATGTGCCTGCTTTGCACTCAGTCCTGGTTTTTGTCTCCATCAAATCATTACCGATAAGCAAGGTTCCGTTGGAGGAGCGTTTTCTTTTCCGCAGAGTGGAGCCTAGAGAGCTTAATGTGTTCCGATGTGTCGCTAGATACGGGTACACAGATGTCCGAAATGAGAATGAACCCTTTGAAGGATTGCTGATAGAAAAATTGAAGGAGTTCATAAGGGATGACTTTTGGTTATCTCAAAGAACCATGCACATAACCGATGGAGAGAAATTGGAAAAGGAAGTTTCTGCTGAGTTAGAAGGTGATGATCATGAGTTGGTTAATGGAAATGGTCATAATGAGAATGAGAACGAAAACGAGAATGTAAAGCAAGTACTTGATGATGATGAAGATCAAAGGAAACAACAAGACGATGTACTGTTGGAGACAGAGATTGAGGCGATTGACAAAGCGTGGCATCGGGGGGTTGTGCACTTAATTGGTGAGAATGAAGTGACGGCTGCGAAAGGAGCTGGGATAGCGAAAAGGATCTTGATTGATTATGCTTACAATTTCTTGAAGAGAAATCTGAGGCAGAGCGACAAAGTGTTTGATATTCCTCACAAGCGCATGTTAAAAGTGGGCATGACTTATGAACTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

812

Amino Acids

91.75

Weight (kDa)

6.53

Isoelectric Point (pI)

33.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	78 - 564	2.9e-169	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	578 - 812	1e-26	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001609)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g30541 FvH4_6g30550 FvH4_c6g00050 FvH4_c6g00050 FvH4_c6g00050
malus_domestica	MD03G1283500.v1.1 MD03G1283600.v1.1 MD10G1230200.v1.1 MD10G1230300.v1.1 MD11G1302600.v1.1 MD11G1302900.v1.1 MD11G1303100.v1.1
prunus_persica	Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1
pyrus_communis	pycom03g22450 pycom03g22460
rosa_chinensis	RchiOBHm_Chr2g0136981 RchiOBHm_Chr5g0020481 RchiOBHm_Chr6g0293251
rosa_laevigata	RLG00000011964 RLG00000033905
rosa_multiflora	Rmu_co8314359.1_g000001 Rmu_sc0001374.1_g000063 Rmu_sc0001374.1_g000064 Rmu_sc0021490.1_g000001 Rmu_ssc0000162.1_g000030
rosa_roxburghii	Rroxscaffold_2G00107800 Rroxscaffold_2G00107830 Rroxscaffold_7G00174310
rosa_rugosa	Rorug02G0336400 Rorug06G0233800 Rorug06G0233800 Rorug06G0233800
rosa_samantha	Rh2AG385800 Rh2BG392700 Rh2CG373000 Rh2DG047100 Rh2DG408800 Rh2DG408900 Rh3DG241600 Rh3DG283500 Rh6AG346700 Rh6BG354100 Rh6CG360600 Rh6DG347100
rosa_wichuraiana	Rw2G003360 Rw2G031570 Rw6G030180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1444
Acc65I	GGTACC	1 cut(s)	1232
AccB1I	GGYRCC	1 cut(s)	1232
AccI	GTMKAC	1 cut(s)	864
AccII	CGCG	2 cut(s)	510, 1796
AccIII	TCCGGA	2 cut(s)	91, 1330
AciI	CCGC	5 cut(s)	58, 508, 1726, 1796, 1891
AclWI	GGATC	6 cut(s)	35, 48, 102, 878, 1029, 2333
AcoI	YGGCCR	2 cut(s)	190, 1018
AcsI	RAATTY	4 cut(s)	520, 1565, 1677, 1770
AcuI	CTGAAG	4 cut(s)	27, 916, 1017, 1768
AcyI	GRCGYC	2 cut(s)	657, 861
AdeI	CACNNNGTG	1 cut(s)	173
AfiI	CCNNNNNNNGG	5 cut(s)	556, 775, 1181, 1897, 1979
AgsI	TTSAA	7 cut(s)	519, 877, 898, 1766, 1979, 2002, 2356
AhdI	GACNNNNNGTC	2 cut(s)	163, 489
AjnI	CCWGG	4 cut(s)	201, 1455, 1732, 1824
AleI	CACNNNNGTG	1 cut(s)	1267
Alw21I	GWGCWC	7 cut(s)	1058, 1119, 1296, 1560, 1668, 1674, 2271
Alw26I	GTCTC	4 cut(s)	258, 1260, 1840, 2216
Alw44I	GTGCAC	2 cut(s)	1292, 2267
AlwI	GGATC	6 cut(s)	35, 48, 102, 878, 1029, 2333
AlwNI	CAGNNNCTG	1 cut(s)	995
Ama87I	CYCGRG	1 cut(s)	1667
Aor13HI	TCCGGA	2 cut(s)	91, 1330
AoxI	GGCC	4 cut(s)	190, 1018, 1172, 1735
ApaLI	GTGCAC	2 cut(s)	1292, 2267
ApeKI	GCWGC	1 cut(s)	2297
ApoI	RAATTY	4 cut(s)	520, 1565, 1677, 1770
ArsI	GACNNNNNNTTYG	4 cut(s)	1114, 1146, 2369, 2401
Asp700I	GAANNNNTTC	3 cut(s)	1139, 1761, 2006
Asp718I	GGTACC	1 cut(s)	1232
AspLEI	GCGC	1 cut(s)	2406
AsuII	TTCGAA	1 cut(s)	1133
AvaI	CYCGRG	1 cut(s)	1667
AxyI	CCTNAGG	1 cut(s)	551
BaeGI	GKGCMC	2 cut(s)	1296, 2271
BalI	TGGCCA	2 cut(s)	192, 1020
BamHI	GGATCC	1 cut(s)	40
BanI	GGYRCC	1 cut(s)	1232
BanII	GRGCYC	1 cut(s)	1668
BarI	GAAGNNNNNNTAC	4 cut(s)	60, 63, 92, 95
BauI	CACGAG	1 cut(s)	1278
BbsI	GAAGAC	2 cut(s)	388, 510
Bbv12I	GWGCWC	7 cut(s)	1058, 1119, 1296, 1560, 1668, 1674, 2271
BbvI	GCAGC	1 cut(s)	2284
BccI	CCATC	4 cut(s)	60, 647, 1847, 2051
BceAI	ACGGC	4 cut(s)	1175, 1429, 1691, 2310
BcgI	CGANNNNNNTGC	2 cut(s)	198, 232
BciT130I	CCWGG	4 cut(s)	203, 1457, 1734, 1826
BclI	TGATCA	2 cut(s)	354, 2104
BcoDI	GTCTC	4 cut(s)	258, 1260, 1840, 2216
BfaI	CTAG	6 cut(s)	233, 540, 1127, 1265, 1905, 1935
BfmI	CTRYAG	2 cut(s)	808, 2435
BglI	GCCNNNNNGGC	1 cut(s)	1196
BisI	GCNGC	3 cut(s)	1726, 1797, 2298
BlsI	GCNGC	3 cut(s)	1727, 1798, 2299
BmcAI	AGTACT	4 cut(s)	78, 605, 1655, 2172
Bme1390I	CCNGG	4 cut(s)	203, 1457, 1734, 1826
BmeRI	GACNNNNNGTC	2 cut(s)	163, 489
BmeT110I	CYCGRG	1 cut(s)	1667
BmiI	GGNNCC	4 cut(s)	42, 1234, 1866, 1902
BmrFI	CCNGG	4 cut(s)	203, 1457, 1734, 1826
BmsI	GCATC	8 cut(s)	324, 569, 681, 765, 940, 1208, 1602, 2263
BpiI	GAAGAC	2 cut(s)	388, 510
Bpu14I	TTCGAA	1 cut(s)	1133
BpuEI	CTTGAG	3 cut(s)	551, 1567, 1679
BsaAI	YACGTR	1 cut(s)	140
BsaBI	GATNNNNATC	3 cut(s)	39, 1641, 2187
BsaHI	GRCGYC	2 cut(s)	657, 861
BsaWI	WCCGGW	3 cut(s)	91, 1091, 1330
BsaXI	ACNNNNNCTCC	2 cut(s)	1526, 1556
Bsc4I	CCNNNNNNNGG	5 cut(s)	556, 775, 1181, 1897, 1979
Bse21I	CCTNAGG	1 cut(s)	551
Bse8I	GATNNNNATC	3 cut(s)	39, 1641, 2187
BseAI	TCCGGA	2 cut(s)	91, 1330
BseBI	CCWGG	4 cut(s)	203, 1457, 1734, 1826
BseGI	GGATG	4 cut(s)	88, 705, 1245, 2023
BseJI	GATNNNNATC	3 cut(s)	39, 1641, 2187
BseLI	CCNNNNNNNGG	5 cut(s)	556, 775, 1181, 1897, 1979
BseMII	CTCAG	5 cut(s)	690, 1071, 1833, 2079, 2357
BseRI	GAGGAG	1 cut(s)	1889
BseSI	GKGCMC	2 cut(s)	1296, 2271
BseXI	GCAGC	1 cut(s)	2284
BseYI	CCCAGC	2 cut(s)	1069, 2309
BsgI	GTGCAG	1 cut(s)	212
Bsh1236I	CGCG	2 cut(s)	510, 1796
BshFI	GGCC	4 cut(s)	192, 1020, 1174, 1737
BshNI	GGYRCC	1 cut(s)	1232
BsiHKAI	GWGCWC	7 cut(s)	1058, 1119, 1296, 1560, 1668, 1674, 2271
BsiHKCI	CYCGRG	1 cut(s)	1667
BsiSI	CCGG	3 cut(s)	92, 1092, 1331
BslFI	GGGAC	1 cut(s)	448
BslI	CCNNNNNNNGG	5 cut(s)	556, 775, 1181, 1897, 1979
BsmAI	GTCTC	4 cut(s)	258, 1260, 1840, 2216
BsmFI	GGGAC	1 cut(s)	448
BsnI	GGCC	4 cut(s)	192, 1020, 1174, 1737
BsoBI	CYCGRG	1 cut(s)	1667
Bsp119I	TTCGAA	1 cut(s)	1133
Bsp1286I	GDGCHC	7 cut(s)	1058, 1119, 1296, 1560, 1668, 1674, 2271
Bsp13I	TCCGGA	2 cut(s)	91, 1330
Bsp143I	GATC	9 cut(s)	34, 40, 94, 354, 883, 1034, 2104, 2188, 2325
Bsp19I	CCATGG	1 cut(s)	187
BspACI	CCGC	5 cut(s)	58, 508, 1726, 1796, 1891
BspANI	GGCC	4 cut(s)	192, 1020, 1174, 1737
BspCNI	CTCAG	5 cut(s)	689, 1070, 1832, 2080, 2358
BspEI	TCCGGA	2 cut(s)	91, 1330
BspFNI	CGCG	2 cut(s)	510, 1796
BspHI	TCATGA	2 cut(s)	1467, 2107
BspLI	GGNNCC	4 cut(s)	42, 1234, 1866, 1902
BspPI	GGATC	6 cut(s)	35, 48, 102, 878, 1029, 2333
BspT104I	TTCGAA	1 cut(s)	1133
BspT107I	GGYRCC	1 cut(s)	1232
BssMI	GATC	9 cut(s)	34, 40, 94, 354, 883, 1034, 2104, 2188, 2325
BssNI	GRCGYC	2 cut(s)	657, 861
BssSI	CACGAG	1 cut(s)	1278
BssT1I	CCWWGG	6 cut(s)	153, 187, 247, 471, 1374, 1573
Bst2BI	CACGAG	1 cut(s)	1278
Bst2UI	CCWGG	4 cut(s)	203, 1457, 1734, 1826
Bst4CI	ACNGT	7 cut(s)	29, 182, 812, 892, 1030, 1254, 2217
Bst6I	CTCTTC	1 cut(s)	2351
BstACI	GRCGYC	2 cut(s)	657, 861
BstBAI	YACGTR	1 cut(s)	140
BstBI	TTCGAA	1 cut(s)	1133
BstC8I	GCNNGC	5 cut(s)	498, 1213, 1356, 1730, 1809
BstDEI	CTNAG	6 cut(s)	551, 676, 1057, 1819, 2088, 2366
BstDSI	CCRYGG	2 cut(s)	187, 1707
BstEII	GGTNACC	1 cut(s)	182
BstF5I	GGATG	4 cut(s)	88, 705, 1245, 2023
BstFNI	CGCG	2 cut(s)	510, 1796
BstHHI	GCGC	1 cut(s)	2406
BstKTI	GATC	9 cut(s)	37, 43, 97, 357, 886, 1037, 2107, 2191, 2328
BstMAI	GTCTC	4 cut(s)	258, 1260, 1840, 2216
BstMBI	GATC	9 cut(s)	34, 40, 94, 354, 883, 1034, 2104, 2188, 2325
BstNI	CCWGG	4 cut(s)	203, 1457, 1734, 1826
BstNSI	RCATGY	2 cut(s)	1732, 2410
BstPI	GGTNACC	1 cut(s)	182
BstSCI	CCNGG	4 cut(s)	201, 1455, 1732, 1824
BstSFI	CTRYAG	2 cut(s)	808, 2435
BstSLI	GKGCMC	2 cut(s)	1296, 2271
BstSNI	TACGTA	1 cut(s)	140
BstUI	CGCG	2 cut(s)	510, 1796
BstV1I	GCAGC	1 cut(s)	2284
BstV2I	GAAGAC	2 cut(s)	388, 510
BstX2I	RGATCY	2 cut(s)	40, 2325
BstYI	RGATCY	2 cut(s)	40, 2325
Bsu36I	CCTNAGG	1 cut(s)	551
BsuRI	GGCC	4 cut(s)	192, 1020, 1174, 1737
BtgI	CCRYGG	2 cut(s)	187, 1707
BtgZI	GCGATG	1 cut(s)	525
BtsCI	GGATG	4 cut(s)	88, 705, 1245, 2023
BtsI	GCAGTG	2 cut(s)	178, 1428
BtsIMutI	CAGTG	3 cut(s)	178, 1065, 1428
Cac8I	GCNNGC	5 cut(s)	498, 1213, 1356, 1730, 1809
CaiI	CAGNNNCTG	1 cut(s)	995
CciI	TCATGA	2 cut(s)	1467, 2107
CfoI	GCGC	1 cut(s)	2406
CseI	GACGC	3 cut(s)	646, 850, 1447
CspCI	CAANNNNNGTGG	4 cut(s)	610, 645, 1381, 1416
DdeI	CTNAG	6 cut(s)	551, 676, 1057, 1819, 2088, 2366
DpnI	GATC	9 cut(s)	36, 42, 96, 356, 885, 1036, 2106, 2190, 2327
DpnII	GATC	9 cut(s)	34, 40, 94, 354, 883, 1034, 2104, 2188, 2325
DraIII	CACNNNGTG	1 cut(s)	173
DrdI	GACNNNNNNGTC	1 cut(s)	1444
DriI	GACNNNNNGTC	2 cut(s)	163, 489
DseDI	GACNNNNNNGTC	1 cut(s)	1444
EaeI	YGGCCR	2 cut(s)	190, 1018
Eam1104I	CTCTTC	1 cut(s)	2351
Eam1105I	GACNNNNNGTC	2 cut(s)	163, 489
EarI	CTCTTC	1 cut(s)	2351
Ecl136II	GAGCTC	1 cut(s)	1666
Eco105I	TACGTA	1 cut(s)	140
Eco130I	CCWWGG	6 cut(s)	153, 187, 247, 471, 1374, 1573
Eco24I	GRGCYC	1 cut(s)	1668
Eco32I	GATATC	2 cut(s)	747, 1583
Eco53kI	GAGCTC	1 cut(s)	1666
Eco57I	CTGAAG	4 cut(s)	27, 916, 1017, 1768
Eco81I	CCTNAGG	1 cut(s)	551
Eco88I	CYCGRG	1 cut(s)	1667
Eco91I	GGTNACC	1 cut(s)	182
EcoICRI	GAGCTC	1 cut(s)	1666
EcoO65I	GGTNACC	1 cut(s)	182
EcoRI	GAATTC	1 cut(s)	1770
EcoRII	CCWGG	4 cut(s)	201, 1455, 1732, 1824
EcoRV	GATATC	2 cut(s)	747, 1583
EcoT14I	CCWWGG	6 cut(s)	153, 187, 247, 471, 1374, 1573
EcoT22I	ATGCAT	1 cut(s)	1411
EcoT38I	GRGCYC	1 cut(s)	1668
ErhI	CCWWGG	6 cut(s)	153, 187, 247, 471, 1374, 1573
FalI	AAGNNNNNCTT	2 cut(s)	380, 412
FaqI	GGGAC	1 cut(s)	448
FauNDI	CATATG	1 cut(s)	1510
FbaI	TGATCA	2 cut(s)	354, 2104
FblI	GTMKAC	1 cut(s)	864
Fnu4HI	GCNGC	3 cut(s)	1726, 1797, 2298
FokI	GGATG	4 cut(s)	75, 712, 1232, 2030
FriOI	GRGCYC	1 cut(s)	1668
Fsp4HI	GCNGC	3 cut(s)	1726, 1797, 2298
FspBI	CTAG	6 cut(s)	233, 540, 1127, 1265, 1905, 1935
GlaI	GCGC	1 cut(s)	2405
GluI	GCNGC	3 cut(s)	1726, 1797, 2298
GsaI	CCCAGC	2 cut(s)	1073, 2313
HaeIII	GGCC	4 cut(s)	192, 1020, 1174, 1737
HapII	CCGG	3 cut(s)	92, 1092, 1331
HgaI	GACGC	3 cut(s)	646, 850, 1447
HhaI	GCGC	1 cut(s)	2406
Hin1I	GRCGYC	2 cut(s)	657, 861
Hin6I	GCGC	1 cut(s)	2404
HinP1I	GCGC	1 cut(s)	2404
HincII	GTYRAC	2 cut(s)	178, 1339
HindII	GTYRAC	2 cut(s)	178, 1339
HindIII	AAGCTT	1 cut(s)	566
HinfI	GANTC	8 cut(s)	144, 149, 164, 484, 490, 1123, 1148, 1368
HpaII	CCGG	3 cut(s)	92, 1092, 1331
Hpy166II	GTNNAC	9 cut(s)	178, 840, 865, 1294, 1324, 1339, 1711, 1947, 2269
Hpy188III	TCNNGA	8 cut(s)	38, 92, 934, 1331, 1468, 2108, 2329, 2353
Hpy8I	GTNNAC	9 cut(s)	178, 840, 865, 1294, 1324, 1339, 1711, 1947, 2269
Hpy99I	CGWCG	3 cut(s)	431, 731, 1450
HpyCH4III	ACNGT	7 cut(s)	29, 182, 812, 892, 1030, 1254, 2217
HpyCH4IV	ACGT	4 cut(s)	139, 393, 437, 1445
HpyF3I	CTNAG	6 cut(s)	551, 676, 1057, 1819, 2088, 2366
HpySE526I	ACGT	4 cut(s)	139, 393, 437, 1445
Hsp92I	GRCGYC	2 cut(s)	657, 861
HspAI	GCGC	1 cut(s)	2404
Kpn2I	TCCGGA	2 cut(s)	91, 1330
KpnI	GGTACC	1 cut(s)	1236
Ksp22I	TGATCA	2 cut(s)	354, 2104
Kzo9I	GATC	9 cut(s)	34, 40, 94, 354, 883, 1034, 2104, 2188, 2325
LmnI	GCTCC	5 cut(s)	203, 1534, 1876, 1900, 2306
Lsp1109I	GCAGC	1 cut(s)	2284
LweI	GCATC	8 cut(s)	324, 569, 681, 765, 940, 1208, 1602, 2263
MaeI	CTAG	6 cut(s)	233, 540, 1127, 1265, 1905, 1935
MaeII	ACGT	4 cut(s)	139, 393, 437, 1445
MaeIII	GTNAC	6 cut(s)	135, 165, 182, 1060, 1369, 2290
MalI	GATC	9 cut(s)	36, 42, 96, 356, 885, 1036, 2106, 2190, 2327
MboI	GATC	9 cut(s)	34, 40, 94, 354, 883, 1034, 2104, 2188, 2325
MflI	RGATCY	2 cut(s)	40, 2325
MhlI	GDGCHC	7 cut(s)	1058, 1119, 1296, 1560, 1668, 1674, 2271
MlsI	TGGCCA	2 cut(s)	192, 1020
MluNI	TGGCCA	2 cut(s)	192, 1020
MlyI	GAGTC	7 cut(s)	143, 173, 478, 499, 1117, 1157, 1377
MmeI	TCCRAC	7 cut(s)	550, 673, 911, 1273, 1345, 1851, 2199
Mox20I	TGGCCA	2 cut(s)	192, 1020
Mph1103I	ATGCAT	1 cut(s)	1411
MroI	TCCGGA	2 cut(s)	91, 1330
MroXI	GAANNNNTTC	3 cut(s)	1139, 1761, 2006
MscI	TGGCCA	2 cut(s)	192, 1020
MseI	TTAA	7 cut(s)	374, 387, 570, 1914, 2118, 2273, 2411
MslI	CAYNNNNRTG	3 cut(s)	1267, 1421, 1472
Msp20I	TGGCCA	2 cut(s)	192, 1020
MspI	CCGG	3 cut(s)	92, 1092, 1331
MspR9I	CCNGG	4 cut(s)	203, 1457, 1734, 1826
MvaI	CCWGG	4 cut(s)	203, 1457, 1734, 1826
MvnI	CGCG	2 cut(s)	510, 1796
NcoI	CCATGG	1 cut(s)	187
NdeI	CATATG	1 cut(s)	1510
NdeII	GATC	9 cut(s)	34, 40, 94, 354, 883, 1034, 2104, 2188, 2325
NlaIV	GGNNCC	4 cut(s)	42, 1234, 1866, 1902
NmuCI	GTSAC	5 cut(s)	165, 182, 1060, 1369, 2290
NsiI	ATGCAT	1 cut(s)	1411
NspI	RCATGY	2 cut(s)	1732, 2410
NspV	TTCGAA	1 cut(s)	1133
OliI	CACNNNNGTG	1 cut(s)	1267
PaeI	GCATGC	1 cut(s)	1732
PaeR7I	CTCGAG	1 cut(s)	1667
PagI	TCATGA	2 cut(s)	1467, 2107
PasI	CCCWGGG	1 cut(s)	202
PcsI	WCGNNNNNNNCGW	3 cut(s)	399, 423, 1445
PdmI	GAANNNNTTC	3 cut(s)	1139, 1761, 2006
PfeI	GAWTC	1 cut(s)	144
PkrI	GCNGC	3 cut(s)	1727, 1798, 2299
PleI	GAGTC	7 cut(s)	143, 172, 478, 498, 1117, 1156, 1376
PpsI	GAGTC	7 cut(s)	143, 172, 478, 498, 1117, 1156, 1376
Ppu21I	YACGTR	1 cut(s)	140
Psp124BI	GAGCTC	1 cut(s)	1668
Psp6I	CCWGG	4 cut(s)	201, 1455, 1732, 1824
PspEI	GGTNACC	1 cut(s)	182
PspFI	CCCAGC	2 cut(s)	1069, 2309
PspGI	CCWGG	4 cut(s)	201, 1455, 1732, 1824
PspN4I	GGNNCC	4 cut(s)	42, 1234, 1866, 1902
PspXI	VCTCGAGB	1 cut(s)	1667
PstNI	CAGNNNCTG	1 cut(s)	995
PsuI	RGATCY	2 cut(s)	40, 2325
RseI	CAYNNNNRTG	3 cut(s)	1267, 1421, 1472
SacI	GAGCTC	1 cut(s)	1668
SaqAI	TTAA	7 cut(s)	374, 387, 570, 1914, 2118, 2273, 2411
SatI	GCNGC	3 cut(s)	1726, 1797, 2298
Sau3AI	GATC	9 cut(s)	34, 40, 94, 354, 883, 1034, 2104, 2188, 2325
ScaI	AGTACT	4 cut(s)	78, 605, 1655, 2172
SchI	GAGTC	7 cut(s)	143, 173, 478, 499, 1117, 1157, 1377
ScrFI	CCNGG	4 cut(s)	203, 1457, 1734, 1826
SduI	GDGCHC	7 cut(s)	1058, 1119, 1296, 1560, 1668, 1674, 2271
SfaNI	GCATC	8 cut(s)	324, 569, 681, 765, 940, 1208, 1602, 2263
SfcI	CTRYAG	2 cut(s)	808, 2435
Sfr274I	CTCGAG	1 cut(s)	1667
SfuI	TTCGAA	1 cut(s)	1133
SlaI	CTCGAG	1 cut(s)	1667
SmiMI	CAYNNNNRTG	3 cut(s)	1267, 1421, 1472
SmlI	CTYRAG	4 cut(s)	530, 1546, 1667, 1694
SmoI	CTYRAG	4 cut(s)	530, 1546, 1667, 1694
SnaBI	TACGTA	1 cut(s)	140
SphI	GCATGC	1 cut(s)	1732
SsiI	CCGC	5 cut(s)	58, 508, 1726, 1796, 1891
SspI	AATATT	1 cut(s)	18
SspMI	CTAG	6 cut(s)	233, 540, 1127, 1265, 1905, 1935
SstI	GAGCTC	1 cut(s)	1668
StyD4I	CCNGG	4 cut(s)	201, 1455, 1732, 1824
StyI	CCWWGG	6 cut(s)	153, 187, 247, 471, 1374, 1573
TaaI	ACNGT	7 cut(s)	29, 182, 812, 892, 1030, 1254, 2217
TaiI	ACGT	4 cut(s)	142, 396, 440, 1448
TaqII	GACCGA	1 cut(s)	740
TatI	WGTACW	6 cut(s)	76, 603, 1542, 1653, 2170, 2212
TauI	GCSGC	2 cut(s)	1728, 1799
TfiI	GAWTC	1 cut(s)	144
Tru1I	TTAA	7 cut(s)	374, 387, 570, 1914, 2118, 2273, 2411
Tru9I	TTAA	7 cut(s)	374, 387, 570, 1914, 2118, 2273, 2411
TscAI	CASTG	3 cut(s)	178, 1065, 1428
TseFI	GTSAC	5 cut(s)	165, 182, 1060, 1369, 2290
TseI	GCWGC	1 cut(s)	2297
Tsp45I	GTSAC	5 cut(s)	165, 182, 1060, 1369, 2290
TspGWI	ACGGA	2 cut(s)	444, 1857
TspRI	CASTG	3 cut(s)	178, 1065, 1428
VneI	GTGCAC	2 cut(s)	1292, 2267
XapI	RAATTY	4 cut(s)	520, 1565, 1677, 1770
XceI	RCATGY	2 cut(s)	1732, 2410
XhoI	CTCGAG	1 cut(s)	1667
XmiI	GTMKAC	1 cut(s)	864
XmnI	GAANNNNTTC	3 cut(s)	1139, 1761, 2006
XspI	CTAG	6 cut(s)	233, 540, 1127, 1265, 1905, 1935
ZrmI	AGTACT	4 cut(s)	78, 605, 1655, 2172
Zsp2I	ATGCAT	1 cut(s)	1411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.