Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr3

Orthogroup ID

OG0001609

Physical Location & Seq

Genomic Coordinates Forward (+)

14125892 .. 14130560

Gene Structure 4669 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000011964

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2532 bp

ATGGCTTTGGACATCATGTTTGGTGTTCTTAGTTTTCACTCGTTTGAGGTCGCATATGTGTTGTCCTGGTTCCTAACCCTACAATTTCGTGAGATGCCTGAAGATGCAATATTAGAAAACGGTACAGATCAGGATCCTAAAAGCCATGACCGCCAAGAAGAAGTAGTAGTACTTCCTCATCATCCGGATCATCAGCAACTCAAAGGCAAGAAACTTTCATGGCAAAAGTTACGTAGATTCGACTCCTTGGACATCGAGTCACGCAGTGTCAACGGTCACCATGGCCATAGCTCCCAGGGTGCAAGTTGGTCGGTGATACTGCACCTAGCATTTCAGAGCCTTGGAATAGTCTATGGAGACATTGGTACATCACCTCTATATGTGTATGCGAGTACCTTCACCAATGGCATCAAGGACATAGATGACATCTTGGGTGTTCTTTCTTTGATCATATACACCATCACCTTAATCCCCTTGATTAAGTACGTCTTCATCGTCCTCCGAGCCAACGATAATGGCGACGGAGGGACGTTCGCACTTTACTCTCTGTTGTGTCGATATGCCAAGGTTGGTTTGACTCCGAGTCAGCAAGCCGAAGACCGCGATGTTTCAAATTTCCAACTTGAGTTGCCTAGCAAACGCCTAAAGAGGGCATCGAAGCTTAAATCAAAATTAGAGAACAGCAGATTTGCCAAGTACTTTTTACTATTCGCCACCATGCTTGGTACTTCTATGGTTATTGGTGATGGCGTCCTCACTCCTTGCATCTCAGTTCTATCGGCTGTTGGAGGGATGAAACAAGCTACATCTGCCCTGACCGACGATATGGTTGTTTGGATATCAGTAGGCATCTTGGTTTGCCTATTCATGGTTCAGAGATTTGGAACTGACAAAGTTGGCTACAGTTTTGCACCAATAATTTGTGTTTGGTTTACCTTCATTGGTGGCATTGGCGTCTACAATTTCTTCAAATACGATCCAACAGTTTTGAAAGCTCTAAATCCACGATACATAGTCGATTACTTCAAGAGAAACAAAGGAGATGCTTGGATTTCCCTCGGTGGCATTGTTCTTGCCATTACAGGAACTGAAGCCCTGTTTGCCGATGTTGGCCACTTCACAGTTCGATCCATTCAGATTAGTATGTGCTCAGTGACTTACCCAGCTCTCCTACTTGCATATACCGGACAAGCCTCTTTTCTGCGTGAGCACAAGACTCTAGTTTCGAACACCTTCTATGAGTCCATACCAAATCCTTTGTATTGGCCTATGTTTGGGGTTGCCGTAATGGCATCAATCATAGCCAGCCAAGCCATGATTTCGGGTACCTTCTCAATCATCCAACAGTCTCTTTCACTAGGGTGTTTCCCTCGTGTGAAAATTGTGCACACATCGACCAAGTATGAAGGACAAGTTTACATTCCGGAGGTCAACTACCTTCTCATGCTGGCTTGTGTTGGAGTCACCTTGGGTTTCAGAAGCACCACAAAGATTGGAAATGCATACGGCATAGCAGTGGTATTTGTAATGACGCTTACGTCGTCCTTCCTGGTGCTAATCATGATTATGATTTGGAAAACCCCAATACTTCTCATTATCTCATATGTTCTTGTCATCGGAAGCGTGGAGCTTATGTACTTGAGTTCGGTGCTCTACAAATTTGACCAAGGTGGATATCTTCCTTTGGCATTTGCCACTTTACTGATGCTCATAATGTTTGTGTGGAACGATGTTCATCGAAGAAAGTACTACTACGAGCTCGAGCACAAAATTTCCCCTGAAAAGCTGAAGCAACTTGCCGTGGACAAAAACTTTTGCCGCATGCCTGGCCTCGCCATGTTCTATTCAGAACTCGTTCAAGGAATTCCTCCAATCTTCAGTCATTACGCGGCAAATGTGCCTGCTTTGCACTCAGTCCTGGTTTTTGTCTCCATCAAATCATTACCGATAAGCAAGGTTCCGTTGGAGGAGCGTTTTCTTTTCCGCAGAGTGGAGCCTAGAGAGCTTAATGTGTTCCGATGTGTCGCTAGATACGGGTACACAGATGTCCGAAATGAGAATGAACCCTTTGAAGGATTGCTGATAGAAAAATTGAAGGAGTTCATAAGAGATGACTTTTGGCTATCTCAAAGAACCATGCACATAACCGATGGAGAGAAATTGGAAAAGGAAGTTTCTGCTGAGTTAGAAGGTGATGATCATGAGTTGGTTAATGGAAATGGTCATAATGAGAATGAGAACGAAAACGAGAATGTAAAGCAAGTACTTGATGATGATGAAGATCAAAGTAAACAACAAGACGATGTACTGTTGGAGACAGAGATTGAGGCGATTGACAAAGCGTGGCATCGGGGGGTTGTGCACTTAATTGGTGAGAATGAAGTGACGGCTGCGAAAGGAGCTGGGATAGCGAAAAGGATCTTGATTGATTATGCTTACAATTTCTTGAAGAGAAATCTGAGGCAGAGTGACAAAGTGTTTGATATTCCTCACAAGCGCATGTTAAAAGTGGGCATGACTTATGAACTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

844

Amino Acids

95.2

Weight (kDa)

6.29

Isoelectric Point (pI)

32.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	109 - 595	4.1e-168	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	609 - 843	9.5e-27	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001609)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g30541 FvH4_6g30550 FvH4_c6g00050 FvH4_c6g00050 FvH4_c6g00050
malus_domestica	MD03G1283500.v1.1 MD03G1283600.v1.1 MD10G1230200.v1.1 MD10G1230300.v1.1 MD11G1302600.v1.1 MD11G1302900.v1.1 MD11G1303100.v1.1
prunus_persica	Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1
pyrus_communis	pycom03g22450 pycom03g22460
rosa_chinensis	RchiOBHm_Chr2g0136981 RchiOBHm_Chr5g0020481 RchiOBHm_Chr6g0293251
rosa_laevigata	RLG00000011964 RLG00000033905
rosa_multiflora	Rmu_co8314359.1_g000001 Rmu_sc0001374.1_g000063 Rmu_sc0001374.1_g000064 Rmu_sc0021490.1_g000001 Rmu_ssc0000162.1_g000030
rosa_roxburghii	Rroxscaffold_2G00107800 Rroxscaffold_2G00107830 Rroxscaffold_7G00174310
rosa_rugosa	Rorug02G0336400 Rorug06G0233800 Rorug06G0233800 Rorug06G0233800
rosa_samantha	Rh2AG385800 Rh2BG392700 Rh2CG373000 Rh2DG047100 Rh2DG408800 Rh2DG408900 Rh3DG241600 Rh3DG283500 Rh6AG346700 Rh6BG354100 Rh6CG360600 Rh6DG347100
rosa_wichuraiana	Rw2G003360 Rw2G031570 Rw6G030180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1537
Acc65I	GGTACC	1 cut(s)	1325
AccB1I	GGYRCC	1 cut(s)	1325
AccI	GTMKAC	1 cut(s)	957
AccII	CGCG	2 cut(s)	603, 1889
AccIII	TCCGGA	2 cut(s)	184, 1423
AciI	CCGC	5 cut(s)	151, 601, 1819, 1889, 1984
AclWI	GGATC	6 cut(s)	128, 141, 195, 971, 1122, 2426
AcoI	YGGCCR	2 cut(s)	283, 1111
AcsI	RAATTY	4 cut(s)	613, 1658, 1770, 1863
AcuI	CTGAAG	4 cut(s)	120, 1110, 1808, 1861
AcyI	GRCGYC	2 cut(s)	750, 954
AdeI	CACNNNGTG	1 cut(s)	266
AfiI	CCNNNNNNNGG	5 cut(s)	649, 868, 1274, 1990, 2072
AgsI	TTSAA	8 cut(s)	612, 970, 991, 1027, 1859, 2072, 2095, 2449
AhdI	GACNNNNNGTC	2 cut(s)	256, 582
AjnI	CCWGG	5 cut(s)	65, 294, 1548, 1825, 1917
AleI	CACNNNNGTG	1 cut(s)	1360
Alw21I	GWGCWC	7 cut(s)	1151, 1212, 1389, 1653, 1761, 1767, 2364
Alw26I	GTCTC	4 cut(s)	351, 1353, 1933, 2309
Alw44I	GTGCAC	2 cut(s)	1385, 2360
AlwI	GGATC	6 cut(s)	128, 141, 195, 971, 1122, 2426
AlwNI	CAGNNNCTG	1 cut(s)	1088
Ama87I	CYCGRG	1 cut(s)	1760
Aor13HI	TCCGGA	2 cut(s)	184, 1423
AoxI	GGCC	4 cut(s)	283, 1111, 1265, 1828
ApaLI	GTGCAC	2 cut(s)	1385, 2360
ApeKI	GCWGC	1 cut(s)	2390
ApoI	RAATTY	4 cut(s)	613, 1658, 1770, 1863
ArsI	GACNNNNNNTTYG	5 cut(s)	34, 1207, 1239, 2462, 2494
Asp700I	GAANNNNTTC	3 cut(s)	1232, 1854, 2099
Asp718I	GGTACC	1 cut(s)	1325
AspLEI	GCGC	1 cut(s)	2499
AsuHPI	GGTGA	9 cut(s)	269, 325, 363, 391, 454, 755, 1456, 2204, 2384
AsuII	TTCGAA	1 cut(s)	1226
AvaI	CYCGRG	1 cut(s)	1760
BaeGI	GKGCMC	2 cut(s)	1389, 2364
BalI	TGGCCA	2 cut(s)	285, 1113
BamHI	GGATCC	1 cut(s)	133
BanI	GGYRCC	1 cut(s)	1325
BanII	GRGCYC	1 cut(s)	1761
BarI	GAAGNNNNNNTAC	4 cut(s)	153, 156, 185, 188
BauI	CACGAG	1 cut(s)	1371
BbsI	GAAGAC	2 cut(s)	481, 603
Bbv12I	GWGCWC	7 cut(s)	1151, 1212, 1389, 1653, 1761, 1767, 2364
BbvI	GCAGC	1 cut(s)	2377
BccI	CCATC	4 cut(s)	467, 740, 1940, 2144
BceAI	ACGGC	4 cut(s)	1268, 1522, 1784, 2403
BcgI	CGANNNNNNTGC	2 cut(s)	291, 325
BciT130I	CCWGG	5 cut(s)	67, 296, 1550, 1827, 1919
BclI	TGATCA	2 cut(s)	447, 2197
BcoDI	GTCTC	4 cut(s)	351, 1353, 1933, 2309
BfaI	CTAG	6 cut(s)	326, 633, 1220, 1358, 1998, 2028
BfmI	CTRYAG	2 cut(s)	901, 2528
BglI	GCCNNNNNGGC	1 cut(s)	1289
BisI	GCNGC	3 cut(s)	1819, 1890, 2391
BlsI	GCNGC	3 cut(s)	1820, 1891, 2392
BmcAI	AGTACT	4 cut(s)	171, 698, 1748, 2265
Bme1390I	CCNGG	5 cut(s)	67, 296, 1550, 1827, 1919
BmeRI	GACNNNNNGTC	2 cut(s)	256, 582
BmeT110I	CYCGRG	1 cut(s)	1760
BmiI	GGNNCC	5 cut(s)	71, 135, 1327, 1959, 1995
BmrFI	CCNGG	5 cut(s)	67, 296, 1550, 1827, 1919
BpiI	GAAGAC	2 cut(s)	481, 603
Bpu14I	TTCGAA	1 cut(s)	1226
BpuEI	CTTGAG	2 cut(s)	644, 1660
BsaAI	YACGTR	1 cut(s)	233
BsaBI	GATNNNNATC	3 cut(s)	132, 1734, 2280
BsaHI	GRCGYC	2 cut(s)	750, 954
BsaWI	WCCGGW	3 cut(s)	184, 1184, 1423
BsaXI	ACNNNNNCTCC	2 cut(s)	1619, 1649
Bsc4I	CCNNNNNNNGG	5 cut(s)	649, 868, 1274, 1990, 2072
Bse8I	GATNNNNATC	3 cut(s)	132, 1734, 2280
BseAI	TCCGGA	2 cut(s)	184, 1423
BseBI	CCWGG	5 cut(s)	67, 296, 1550, 1827, 1919
BseGI	GGATG	3 cut(s)	181, 798, 1338
BseJI	GATNNNNATC	3 cut(s)	132, 1734, 2280
BseLI	CCNNNNNNNGG	5 cut(s)	649, 868, 1274, 1990, 2072
BseMII	CTCAG	5 cut(s)	783, 1164, 1926, 2172, 2450
BseRI	GAGGAG	1 cut(s)	1982
BseSI	GKGCMC	2 cut(s)	1389, 2364
BseXI	GCAGC	1 cut(s)	2377
BseYI	CCCAGC	2 cut(s)	1162, 2402
BsgI	GTGCAG	1 cut(s)	305
Bsh1236I	CGCG	2 cut(s)	603, 1889
BshFI	GGCC	4 cut(s)	285, 1113, 1267, 1830
BshNI	GGYRCC	1 cut(s)	1325
BsiHKAI	GWGCWC	7 cut(s)	1151, 1212, 1389, 1653, 1761, 1767, 2364
BsiHKCI	CYCGRG	1 cut(s)	1760
BsiSI	CCGG	3 cut(s)	185, 1185, 1424
BslFI	GGGAC	1 cut(s)	541
BslI	CCNNNNNNNGG	5 cut(s)	649, 868, 1274, 1990, 2072
BsmAI	GTCTC	4 cut(s)	351, 1353, 1933, 2309
BsmFI	GGGAC	1 cut(s)	541
BsnI	GGCC	4 cut(s)	285, 1113, 1267, 1830
BsoBI	CYCGRG	1 cut(s)	1760
Bsp119I	TTCGAA	1 cut(s)	1226
Bsp1286I	GDGCHC	7 cut(s)	1151, 1212, 1389, 1653, 1761, 1767, 2364
Bsp13I	TCCGGA	2 cut(s)	184, 1423
Bsp143I	GATC	9 cut(s)	127, 133, 187, 447, 976, 1127, 2197, 2281, 2418
Bsp19I	CCATGG	1 cut(s)	280
BspACI	CCGC	5 cut(s)	151, 601, 1819, 1889, 1984
BspANI	GGCC	4 cut(s)	285, 1113, 1267, 1830
BspCNI	CTCAG	5 cut(s)	782, 1163, 1925, 2173, 2451
BspEI	TCCGGA	2 cut(s)	184, 1423
BspFNI	CGCG	2 cut(s)	603, 1889
BspHI	TCATGA	2 cut(s)	1560, 2200
BspLI	GGNNCC	5 cut(s)	71, 135, 1327, 1959, 1995
BspPI	GGATC	6 cut(s)	128, 141, 195, 971, 1122, 2426
BspT104I	TTCGAA	1 cut(s)	1226
BspT107I	GGYRCC	1 cut(s)	1325
BssMI	GATC	9 cut(s)	127, 133, 187, 447, 976, 1127, 2197, 2281, 2418
BssNI	GRCGYC	2 cut(s)	750, 954
BssSI	CACGAG	1 cut(s)	1371
BssT1I	CCWWGG	6 cut(s)	246, 280, 340, 564, 1467, 1666
Bst2BI	CACGAG	1 cut(s)	1371
Bst2UI	CCWGG	5 cut(s)	67, 296, 1550, 1827, 1919
Bst4CI	ACNGT	7 cut(s)	122, 275, 905, 985, 1123, 1347, 2310
Bst6I	CTCTTC	1 cut(s)	2444
BstACI	GRCGYC	2 cut(s)	750, 954
BstBAI	YACGTR	1 cut(s)	233
BstBI	TTCGAA	1 cut(s)	1226
BstC8I	GCNNGC	5 cut(s)	591, 1306, 1449, 1823, 1902
BstDEI	CTNAG	6 cut(s)	29, 769, 1150, 1912, 2181, 2459
BstDSI	CCRYGG	2 cut(s)	280, 1800
BstEII	GGTNACC	1 cut(s)	275
BstF5I	GGATG	3 cut(s)	181, 798, 1338
BstFNI	CGCG	2 cut(s)	603, 1889
BstHHI	GCGC	1 cut(s)	2499
BstKTI	GATC	9 cut(s)	130, 136, 190, 450, 979, 1130, 2200, 2284, 2421
BstMAI	GTCTC	4 cut(s)	351, 1353, 1933, 2309
BstMBI	GATC	9 cut(s)	127, 133, 187, 447, 976, 1127, 2197, 2281, 2418
BstNI	CCWGG	5 cut(s)	67, 296, 1550, 1827, 1919
BstNSI	RCATGY	2 cut(s)	1825, 2503
BstPI	GGTNACC	1 cut(s)	275
BstSCI	CCNGG	5 cut(s)	65, 294, 1548, 1825, 1917
BstSFI	CTRYAG	2 cut(s)	901, 2528
BstSLI	GKGCMC	2 cut(s)	1389, 2364
BstSNI	TACGTA	1 cut(s)	233
BstUI	CGCG	2 cut(s)	603, 1889
BstV1I	GCAGC	1 cut(s)	2377
BstV2I	GAAGAC	2 cut(s)	481, 603
BstX2I	RGATCY	2 cut(s)	133, 2418
BstYI	RGATCY	2 cut(s)	133, 2418
BsuRI	GGCC	4 cut(s)	285, 1113, 1267, 1830
BtgI	CCRYGG	2 cut(s)	280, 1800
BtgZI	GCGATG	1 cut(s)	618
BtsCI	GGATG	3 cut(s)	181, 798, 1338
BtsI	GCAGTG	2 cut(s)	271, 1521
BtsIMutI	CAGTG	3 cut(s)	271, 1158, 1521
Cac8I	GCNNGC	5 cut(s)	591, 1306, 1449, 1823, 1902
CaiI	CAGNNNCTG	1 cut(s)	1088
CciI	TCATGA	2 cut(s)	1560, 2200
CfoI	GCGC	1 cut(s)	2499
CseI	GACGC	3 cut(s)	739, 943, 1540
CspCI	CAANNNNNGTGG	4 cut(s)	703, 738, 1474, 1509
DdeI	CTNAG	6 cut(s)	29, 769, 1150, 1912, 2181, 2459
DpnI	GATC	9 cut(s)	129, 135, 189, 449, 978, 1129, 2199, 2283, 2420
DpnII	GATC	9 cut(s)	127, 133, 187, 447, 976, 1127, 2197, 2281, 2418
DraIII	CACNNNGTG	1 cut(s)	266
DrdI	GACNNNNNNGTC	1 cut(s)	1537
DriI	GACNNNNNGTC	2 cut(s)	256, 582
DseDI	GACNNNNNNGTC	1 cut(s)	1537
EaeI	YGGCCR	2 cut(s)	283, 1111
Eam1104I	CTCTTC	1 cut(s)	2444
Eam1105I	GACNNNNNGTC	2 cut(s)	256, 582
EarI	CTCTTC	1 cut(s)	2444
Ecl136II	GAGCTC	1 cut(s)	1759
Eco105I	TACGTA	1 cut(s)	233
Eco130I	CCWWGG	6 cut(s)	246, 280, 340, 564, 1467, 1666
Eco24I	GRGCYC	1 cut(s)	1761
Eco32I	GATATC	2 cut(s)	840, 1676
Eco53kI	GAGCTC	1 cut(s)	1759
Eco57I	CTGAAG	4 cut(s)	120, 1110, 1808, 1861
Eco88I	CYCGRG	1 cut(s)	1760
Eco91I	GGTNACC	1 cut(s)	275
EcoICRI	GAGCTC	1 cut(s)	1759
EcoO65I	GGTNACC	1 cut(s)	275
EcoRI	GAATTC	1 cut(s)	1863
EcoRII	CCWGG	5 cut(s)	65, 294, 1548, 1825, 1917
EcoRV	GATATC	2 cut(s)	840, 1676
EcoT14I	CCWWGG	6 cut(s)	246, 280, 340, 564, 1467, 1666
EcoT22I	ATGCAT	1 cut(s)	1504
EcoT38I	GRGCYC	1 cut(s)	1761
ErhI	CCWWGG	6 cut(s)	246, 280, 340, 564, 1467, 1666
FalI	AAGNNNNNCTT	2 cut(s)	473, 505
FaqI	GGGAC	1 cut(s)	541
FauNDI	CATATG	2 cut(s)	55, 1603
FbaI	TGATCA	2 cut(s)	447, 2197
FblI	GTMKAC	1 cut(s)	957
Fnu4HI	GCNGC	3 cut(s)	1819, 1890, 2391
FokI	GGATG	3 cut(s)	168, 805, 1325
FriOI	GRGCYC	1 cut(s)	1761
Fsp4HI	GCNGC	3 cut(s)	1819, 1890, 2391
FspBI	CTAG	6 cut(s)	326, 633, 1220, 1358, 1998, 2028
GlaI	GCGC	1 cut(s)	2498
GluI	GCNGC	3 cut(s)	1819, 1890, 2391
GsaI	CCCAGC	2 cut(s)	1166, 2406
HaeIII	GGCC	4 cut(s)	285, 1113, 1267, 1830
HapII	CCGG	3 cut(s)	185, 1185, 1424
HgaI	GACGC	3 cut(s)	739, 943, 1540
HhaI	GCGC	1 cut(s)	2499
Hin1I	GRCGYC	2 cut(s)	750, 954
Hin6I	GCGC	1 cut(s)	2497
HinP1I	GCGC	1 cut(s)	2497
HincII	GTYRAC	2 cut(s)	271, 1432
HindII	GTYRAC	2 cut(s)	271, 1432
HindIII	AAGCTT	1 cut(s)	659
HinfI	GANTC	8 cut(s)	237, 242, 257, 577, 583, 1216, 1241, 1461
HpaII	CCGG	3 cut(s)	185, 1185, 1424
HphI	GGTGA	9 cut(s)	269, 325, 363, 391, 454, 755, 1456, 2204, 2384
Hpy188III	TCNNGA	9 cut(s)	89, 131, 185, 1027, 1424, 1561, 2201, 2422, 2446
Hpy99I	CGWCG	3 cut(s)	524, 824, 1543
HpyCH4III	ACNGT	7 cut(s)	122, 275, 905, 985, 1123, 1347, 2310
HpyCH4IV	ACGT	4 cut(s)	232, 486, 530, 1538
HpyF3I	CTNAG	6 cut(s)	29, 769, 1150, 1912, 2181, 2459
HpySE526I	ACGT	4 cut(s)	232, 486, 530, 1538
Hsp92I	GRCGYC	2 cut(s)	750, 954
HspAI	GCGC	1 cut(s)	2497
Kpn2I	TCCGGA	2 cut(s)	184, 1423
KpnI	GGTACC	1 cut(s)	1329
Ksp22I	TGATCA	2 cut(s)	447, 2197
Kzo9I	GATC	9 cut(s)	127, 133, 187, 447, 976, 1127, 2197, 2281, 2418
LmnI	GCTCC	5 cut(s)	296, 1627, 1969, 1993, 2399
Lsp1109I	GCAGC	1 cut(s)	2377
MaeI	CTAG	6 cut(s)	326, 633, 1220, 1358, 1998, 2028
MaeII	ACGT	4 cut(s)	232, 486, 530, 1538
MaeIII	GTNAC	7 cut(s)	228, 258, 275, 1153, 1462, 2383, 2468
MalI	GATC	9 cut(s)	129, 135, 189, 449, 978, 1129, 2199, 2283, 2420
MboI	GATC	9 cut(s)	127, 133, 187, 447, 976, 1127, 2197, 2281, 2418
MflI	RGATCY	2 cut(s)	133, 2418
MhlI	GDGCHC	7 cut(s)	1151, 1212, 1389, 1653, 1761, 1767, 2364
MlsI	TGGCCA	2 cut(s)	285, 1113
MluNI	TGGCCA	2 cut(s)	285, 1113
MlyI	GAGTC	7 cut(s)	236, 266, 571, 592, 1210, 1250, 1470
MmeI	TCCRAC	7 cut(s)	643, 766, 1004, 1366, 1438, 1944, 2292
Mox20I	TGGCCA	2 cut(s)	285, 1113
Mph1103I	ATGCAT	1 cut(s)	1504
MroI	TCCGGA	2 cut(s)	184, 1423
MroXI	GAANNNNTTC	3 cut(s)	1232, 1854, 2099
MscI	TGGCCA	2 cut(s)	285, 1113
MseI	TTAA	7 cut(s)	467, 480, 663, 2007, 2211, 2366, 2504
MslI	CAYNNNNRTG	3 cut(s)	1360, 1514, 1565
Msp20I	TGGCCA	2 cut(s)	285, 1113
MspI	CCGG	3 cut(s)	185, 1185, 1424
MspR9I	CCNGG	5 cut(s)	67, 296, 1550, 1827, 1919
MvaI	CCWGG	5 cut(s)	67, 296, 1550, 1827, 1919
MvnI	CGCG	2 cut(s)	603, 1889
NcoI	CCATGG	1 cut(s)	280
NdeI	CATATG	2 cut(s)	55, 1603
NdeII	GATC	9 cut(s)	127, 133, 187, 447, 976, 1127, 2197, 2281, 2418
NlaIV	GGNNCC	5 cut(s)	71, 135, 1327, 1959, 1995
NmuCI	GTSAC	6 cut(s)	258, 275, 1153, 1462, 2383, 2468
NsiI	ATGCAT	1 cut(s)	1504
NspI	RCATGY	2 cut(s)	1825, 2503
NspV	TTCGAA	1 cut(s)	1226
OliI	CACNNNNGTG	1 cut(s)	1360
PaeI	GCATGC	1 cut(s)	1825
PaeR7I	CTCGAG	1 cut(s)	1760
PagI	TCATGA	2 cut(s)	1560, 2200
PasI	CCCWGGG	1 cut(s)	295
PcsI	WCGNNNNNNNCGW	3 cut(s)	492, 516, 1538
PdmI	GAANNNNTTC	3 cut(s)	1232, 1854, 2099
PfeI	GAWTC	1 cut(s)	237
PkrI	GCNGC	3 cut(s)	1820, 1891, 2392
PleI	GAGTC	7 cut(s)	236, 265, 571, 591, 1210, 1249, 1469
PpsI	GAGTC	7 cut(s)	236, 265, 571, 591, 1210, 1249, 1469
Ppu21I	YACGTR	1 cut(s)	233
Psp124BI	GAGCTC	1 cut(s)	1761
Psp6I	CCWGG	5 cut(s)	65, 294, 1548, 1825, 1917
PspEI	GGTNACC	1 cut(s)	275
PspFI	CCCAGC	2 cut(s)	1162, 2402
PspGI	CCWGG	5 cut(s)	65, 294, 1548, 1825, 1917
PspN4I	GGNNCC	5 cut(s)	71, 135, 1327, 1959, 1995
PspXI	VCTCGAGB	1 cut(s)	1760
PstNI	CAGNNNCTG	1 cut(s)	1088
PsuI	RGATCY	2 cut(s)	133, 2418
RseI	CAYNNNNRTG	3 cut(s)	1360, 1514, 1565
SacI	GAGCTC	1 cut(s)	1761
SaqAI	TTAA	7 cut(s)	467, 480, 663, 2007, 2211, 2366, 2504
SatI	GCNGC	3 cut(s)	1819, 1890, 2391
Sau3AI	GATC	9 cut(s)	127, 133, 187, 447, 976, 1127, 2197, 2281, 2418
ScaI	AGTACT	4 cut(s)	171, 698, 1748, 2265
SchI	GAGTC	7 cut(s)	236, 266, 571, 592, 1210, 1250, 1470
ScrFI	CCNGG	5 cut(s)	67, 296, 1550, 1827, 1919
SduI	GDGCHC	7 cut(s)	1151, 1212, 1389, 1653, 1761, 1767, 2364
SfcI	CTRYAG	2 cut(s)	901, 2528
Sfr274I	CTCGAG	1 cut(s)	1760
SfuI	TTCGAA	1 cut(s)	1226
SlaI	CTCGAG	1 cut(s)	1760
SmiMI	CAYNNNNRTG	3 cut(s)	1360, 1514, 1565
SmlI	CTYRAG	3 cut(s)	623, 1639, 1760
SmoI	CTYRAG	3 cut(s)	623, 1639, 1760
SnaBI	TACGTA	1 cut(s)	233
SphI	GCATGC	1 cut(s)	1825
SsiI	CCGC	5 cut(s)	151, 601, 1819, 1889, 1984
SspI	AATATT	1 cut(s)	111
SspMI	CTAG	6 cut(s)	326, 633, 1220, 1358, 1998, 2028
SstI	GAGCTC	1 cut(s)	1761
StyD4I	CCNGG	5 cut(s)	65, 294, 1548, 1825, 1917
StyI	CCWWGG	6 cut(s)	246, 280, 340, 564, 1467, 1666
TaaI	ACNGT	7 cut(s)	122, 275, 905, 985, 1123, 1347, 2310
TaiI	ACGT	4 cut(s)	235, 489, 533, 1541
TaqII	GACCGA	1 cut(s)	833
TatI	WGTACW	6 cut(s)	169, 696, 1635, 1746, 2263, 2305
TauI	GCSGC	2 cut(s)	1821, 1892
TfiI	GAWTC	1 cut(s)	237
Tru1I	TTAA	7 cut(s)	467, 480, 663, 2007, 2211, 2366, 2504
Tru9I	TTAA	7 cut(s)	467, 480, 663, 2007, 2211, 2366, 2504
TscAI	CASTG	3 cut(s)	271, 1158, 1521
TseFI	GTSAC	6 cut(s)	258, 275, 1153, 1462, 2383, 2468
TseI	GCWGC	1 cut(s)	2390
Tsp45I	GTSAC	6 cut(s)	258, 275, 1153, 1462, 2383, 2468
TspGWI	ACGGA	2 cut(s)	537, 1950
TspRI	CASTG	3 cut(s)	271, 1158, 1521
VneI	GTGCAC	2 cut(s)	1385, 2360
XapI	RAATTY	4 cut(s)	613, 1658, 1770, 1863
XceI	RCATGY	2 cut(s)	1825, 2503
XhoI	CTCGAG	1 cut(s)	1760
XmiI	GTMKAC	1 cut(s)	957
XmnI	GAANNNNTTC	3 cut(s)	1232, 1854, 2099
XspI	CTAG	6 cut(s)	326, 633, 1220, 1358, 1998, 2028
ZrmI	AGTACT	4 cut(s)	171, 698, 1748, 2265
Zsp2I	ATGCAT	1 cut(s)	1504

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.