Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0001609

Physical Location & Seq

Genomic Coordinates Reverse (-)

41824993 .. 41831341

Gene Structure 6349 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1302900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2277 bp

ATGTCTGATGAAGAGGTAGTAGTGGAAAACCCTACAGATGAGGGAAGCCAAGATCATCAGGAAGTTTCTGATCATCATGAGCATCATAAGGATCAACTCAAACGGAAGAAACTTTCATGGCAAAAGTTGCGAAGAAACGACTCCTTGGACCTCGAGTCACGCCGTTTCACAGCTCCCCATGGCCATGCCTCCAAGGGTGCGGACTGGTCGGTGATAATGCACTTGGCATTTCAGAGCATTGGCATAGTTTATGGGGACATTGGTACATCACCTCTCTATGTGTATGCGAGCACCTTCACCAAAGGCATCAACCATAGCGATGACATTTTGGGTGTTCTTTCTTTGATCTTTTACACACTCACCTTAATCCCTCTGATTAAGTATGTTTTAATCGTCTTACGGGCCAATGATAACGGTGATGGGACGTTCGCACTATACTCTTTGCTGTGTCGATATGCCAAGGTTGGTTTGACTCCGAGTCAACAAGCAGAGGATCGTGATGTTTCAAAATTTCAGCTTGAGTTGCCTACCAAAAGTGTAAAGAGGGCATCTAGGCTTAAATCTTCATTGGAGAACAGCCCATTTGCCAAATTATTTCTACTATTCGCCACCATGCTTGGTACTTCCATGGTCATTAGTGATGGTGTCCTCACTCCTTGCATGTCAGTTCTGTCGGCTGTTGGAGGAATCAAAGAAGCCACATCTGCAATGACACAAGGTCGGGTTGTTTTGATATCAGTAGTCATCTTGATTGCCCTATTCATGGTTCAAAGATTTGGAACTGATAAAGTAGGCTACATTTTTGCACCAATTATTTGCATTTGGTTTACCATGATTGGTGGAATTGGCGTCTACAACTTCATCGAGTTTGATCCGACGGTGGTCAAGGCTCTAAATCCACAATACATAGTAGATTACTTCAGGAGGAACAAAAAAGACGCTTGGATTTCTCTTGGTGGCATTGTCTTAGCCATAACAGGAACTGAAGCTTTATTTGCTGACGTGGGACACTTCACAGTACAATCCATTCAAATAAGTATGTGCACAATTACTTATCCAGCTATCATATTGGCATACACTGGACAAGCCTCTTTTCTTCGCAAGCACCATCTTCTTGTTGCAGATACCTTCTTCAAGTCCATACCAAAGCCTTTGTATTGGCCTATGTTTGTAGTGGCTGTAATGGCAGCGATCATAGCTAGTCAAGCTATGATTTCAGGGACTTTCTCTATAATCCAACAATCACTATCATTAGGGTGTTTCCCTCGTGTGAAAATCGTGCACACATCAACCAAGTATGCCGGACAAGTTTATATTCCGGAAGTGAACTACCTTCTCATGTTAGCTTGTGTTGGAGTCACTTTAGGGTTTCGAAGCACTGCAAAGATCGGAAATGCATATGGTGTAGCAGTGGTGTTTGTAACGACGCTTACATCATCCTTCCTAGTGCTAATCATGATCATGATATGGAAGACCAACATATTCCTCGTCATCTCATATGTTCTTGTTATTGGAAGTGTAGAGCTTCTGTATCTAAGTTCAGTGCTCTACAAACTTGACCAGGGCGGATATCTTCCCCTCGCGTTTGCTACATTGTTGATGTTTATAATGTTTGTGTGGAATGATGTGCATCGAAGAAAGTACTATTACGAGCTTGAACACAAAATTTCTCCCGAACAACTCAAGGAAATTGCTGTTGATGCAAACTTTTCTCGTATGCCTGGCCTCGCCATGTTCTATTCGGAACTTGTTCAAGGCATTCCGCCAATCTTCAATCATTACGCTGCTAATGTGCCTGCATTGCACTCCGTCCTTGTTTTTGTCTCAATCAAATCGTTGCCTATAAGCAAGGTTCCATTGGAAGAGCGCTTTCTTTTTCGGCAAGTAGAGGCTAAGGATCTGAATGTGTTCCGATGCGTTGCTAGATATGGGTACACCGATGTTCGCAATGAGAACGAACCCTTCGAAGGATTGTTGGTGGAGAAGTTGAAAGAGTTCATAAAGGATAATTTTTGGATATCTCAAACAAATATTATGCACAGTAGCGTTTATGGGGATACCAAGTTGGAGATCGAGGAAGAATTCGAAGACAGTACTTTGGCTAACGGTGGAGAGAAAGGAAAGGAGGATTTGAAGCAGCAAGTTGATGATCATGATAAGCAACAAGATTTGTTGGACAGAGAGATTGAGGCAATAGACAAAGCATGGAGGCGGGGAGTTGTTCATTTGATTGGGGAAAATGAAGTGACGGCTGCCAAAGGAGCTGGCATGGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006814 GO:0008150 GO:0008324 GO:0009674 GO:0009987 GO:0010107 GO:0015075 GO:0015077 GO:0015079 GO:0015081 GO:0015291 GO:0015293 GO:0015294 GO:0015318 GO:0015370 GO:0015672 GO:0016020 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0034220 GO:0035725 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0071804 GO:0071805 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

K_trans

Protein Analysis

759

Amino Acids

84.89

Weight (kDa)

6.48

Isoelectric Point (pI)

35.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
K_trans	PF02705	75 - 560	1.3e-160	K+ potassium transporter integral membrane domain
K_trans_C	PF22776	574 - 681	9.4e-18	K+ potassium transporter C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001609)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g30541 FvH4_6g30550 FvH4_c6g00050 FvH4_c6g00050 FvH4_c6g00050
malus_domestica	MD03G1283500.v1.1 MD03G1283600.v1.1 MD10G1230200.v1.1 MD10G1230300.v1.1 MD11G1302600.v1.1 MD11G1302900.v1.1 MD11G1303100.v1.1
prunus_persica	Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1 Prupe.8G256200_v2.0.a1
pyrus_communis	pycom03g22450 pycom03g22460
rosa_chinensis	RchiOBHm_Chr2g0136981 RchiOBHm_Chr5g0020481 RchiOBHm_Chr6g0293251
rosa_laevigata	RLG00000011964 RLG00000033905
rosa_multiflora	Rmu_co8314359.1_g000001 Rmu_sc0001374.1_g000063 Rmu_sc0001374.1_g000064 Rmu_sc0021490.1_g000001 Rmu_ssc0000162.1_g000030
rosa_roxburghii	Rroxscaffold_2G00107800 Rroxscaffold_2G00107830 Rroxscaffold_7G00174310
rosa_rugosa	Rorug02G0336400 Rorug06G0233800 Rorug06G0233800 Rorug06G0233800
rosa_samantha	Rh2AG385800 Rh2BG392700 Rh2CG373000 Rh2DG047100 Rh2DG408800 Rh2DG408900 Rh3DG241600 Rh3DG283500 Rh6AG346700 Rh6BG354100 Rh6CG360600 Rh6DG347100
rosa_wichuraiana	Rw2G003360 Rw2G031570 Rw6G030180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1607
AccI	GTMKAC	1 cut(s)	852
AccII	CGCG	1 cut(s)	1583
AccIII	TCCGGA	1 cut(s)	1318
AciI	CCGC	4 cut(s)	200, 1566, 1763, 2212
AclWI	GGATC	4 cut(s)	99, 501, 866, 1905
AcoI	YGGCCR	1 cut(s)	181
AcsI	RAATTY	3 cut(s)	509, 1665, 2081
AcuI	CTGAAG	2 cut(s)	904, 1005
AcyI	GRCGYC	1 cut(s)	849
AfaI	GTAC	6 cut(s)	265, 622, 1020, 1643, 1934, 2095
AfeI	AGCGCT	1 cut(s)	1868
AfiI	CCNNNNNNNGG	2 cut(s)	763, 1967
AgsI	TTSAA	9 cut(s)	507, 770, 1031, 1135, 1658, 1754, 1774, 1990, 2134
AhdI	GACNNNNNGTC	3 cut(s)	154, 477, 717
AjiI	CACGTC	1 cut(s)	1003
AjnI	CCWGG	2 cut(s)	1560, 1720
Alw21I	GWGCWC	4 cut(s)	293, 1046, 1284, 1548
Alw26I	GTCTC	1 cut(s)	1828
Alw44I	GTGCAC	2 cut(s)	1042, 1280
AlwI	GGATC	4 cut(s)	99, 501, 866, 1905
AlwNI	CAGNNNCTG	1 cut(s)	983
Ama87I	CYCGRG	1 cut(s)	152
Aor13HI	TCCGGA	1 cut(s)	1318
Aor51HI	AGCGCT	1 cut(s)	1868
AoxI	GGCC	4 cut(s)	181, 402, 1160, 1723
ApaLI	GTGCAC	2 cut(s)	1042, 1280
ApeKI	GCWGC	4 cut(s)	1187, 1784, 2137, 2252
ApoI	RAATTY	3 cut(s)	509, 1665, 2081
Asp700I	GAANNNNTTC	3 cut(s)	1749, 1907, 1994
AspLEI	GCGC	1 cut(s)	1869
AspS9I	GGNCC	2 cut(s)	148, 402
AsuHPI	GGTGA	5 cut(s)	223, 261, 289, 352, 428
AsuII	TTCGAA	3 cut(s)	1372, 1965, 2085
AvaI	CYCGRG	1 cut(s)	152
AvaII	GGWCC	1 cut(s)	148
BaeGI	GKGCMC	2 cut(s)	1046, 1284
BalI	TGGCCA	1 cut(s)	183
BauI	CACGAG	1 cut(s)	1266
BbsI	GAAGAC	2 cut(s)	1478, 2094
Bbv12I	GWGCWC	4 cut(s)	293, 1046, 1284, 1548
BbvI	GCAGC	4 cut(s)	1199, 1771, 2149, 2239
BccI	CCATC	3 cut(s)	413, 635, 1116
BceAI	ACGGC	2 cut(s)	147, 2265
BciT130I	CCWGG	2 cut(s)	1562, 1722
BciVI	GTATCC	1 cut(s)	2050
BclI	TGATCA	3 cut(s)	70, 1458, 2149
BcoDI	GTCTC	1 cut(s)	1828
BfaI	CTAG	4 cut(s)	552, 1200, 1445, 1923
BfmI	CTRYAG	1 cut(s)	33
BfoI	RGCGCY	1 cut(s)	1870
BfuI	GTATCC	1 cut(s)	2050
BisI	GCNGC	4 cut(s)	1188, 1785, 2138, 2253
BlsI	GCNGC	4 cut(s)	1189, 1786, 2139, 2254
BmcAI	AGTACT	2 cut(s)	1643, 2095
Bme1390I	CCNGG	2 cut(s)	1562, 1722
Bme18I	GGWCC	1 cut(s)	148
BmeRI	GACNNNNNGTC	3 cut(s)	154, 477, 717
BmeT110I	CYCGRG	1 cut(s)	152
BmgBI	CACGTC	1 cut(s)	1003
BmgT120I	GGNCC	2 cut(s)	148, 402
BmiI	GGNNCC	1 cut(s)	1854
BmrFI	CCNGG	2 cut(s)	1562, 1722
BmsI	GCATC	6 cut(s)	91, 315, 557, 1639, 1690, 1904
BoxI	GACNNNNGTC	1 cut(s)	881
BpiI	GAAGAC	2 cut(s)	1478, 2094
Bpu10I	CCTNAGC	1 cut(s)	1893
Bpu14I	TTCGAA	3 cut(s)	1372, 1965, 2085
BpuEI	CTTGAG	2 cut(s)	539, 1667
BsaBI	GATNNNNATC	1 cut(s)	1629
BsaHI	GRCGYC	1 cut(s)	849
BsaJI	CCNNGG	6 cut(s)	144, 178, 192, 459, 627, 1561
BsaWI	WCCGGW	1 cut(s)	1318
Bsc4I	CCNNNNNNNGG	2 cut(s)	763, 1967
Bse1I	ACTGG	2 cut(s)	209, 1084
Bse3DI	GCAATG	3 cut(s)	714, 1799, 1954
Bse8I	GATNNNNATC	1 cut(s)	1629
BseAI	TCCGGA	1 cut(s)	1318
BseBI	CCWGG	2 cut(s)	1562, 1722
BseDI	CCNNGG	6 cut(s)	144, 178, 192, 459, 627, 1561
BseGI	GGATG	1 cut(s)	1436
BseJI	GATNNNNATC	1 cut(s)	1629
BseLI	CCNNNNNNNGG	2 cut(s)	763, 1967
BseMI	GCAATG	3 cut(s)	714, 1799, 1954
BseNI	ACTGG	2 cut(s)	209, 1084
BseSI	GKGCMC	2 cut(s)	1046, 1284
BseXI	GCAGC	4 cut(s)	1199, 1771, 2149, 2239
Bsh1236I	CGCG	1 cut(s)	1583
BshFI	GGCC	4 cut(s)	183, 404, 1162, 1725
BsiHKAI	GWGCWC	4 cut(s)	293, 1046, 1284, 1548
BsiHKCI	CYCGRG	1 cut(s)	152
BsiSI	CCGG	2 cut(s)	1302, 1319
BslFI	GGGAC	4 cut(s)	269, 436, 1020, 1234
BslI	CCNNNNNNNGG	2 cut(s)	763, 1967
BsmAI	GTCTC	1 cut(s)	1828
BsmFI	GGGAC	4 cut(s)	269, 436, 1020, 1234
BsmI	GAATGC	1 cut(s)	1758
BsnI	GGCC	4 cut(s)	183, 404, 1162, 1725
BsoBI	CYCGRG	1 cut(s)	152
Bsp119I	TTCGAA	3 cut(s)	1372, 1965, 2085
Bsp1286I	GDGCHC	4 cut(s)	293, 1046, 1284, 1548
Bsp13I	TCCGGA	1 cut(s)	1318
Bsp19I	CCATGG	2 cut(s)	178, 627
BspACI	CCGC	4 cut(s)	200, 1566, 1763, 2212
BspANI	GGCC	4 cut(s)	183, 404, 1162, 1725
BspEI	TCCGGA	1 cut(s)	1318
BspFNI	CGCG	1 cut(s)	1583
BspHI	TCATGA	4 cut(s)	76, 1455, 1461, 2152
BspLI	GGNNCC	1 cut(s)	1854
BspPI	GGATC	4 cut(s)	99, 501, 866, 1905
BspQI	GCTCTTC	1 cut(s)	1857
BspT104I	TTCGAA	3 cut(s)	1372, 1965, 2085
BsrDI	GCAATG	3 cut(s)	714, 1799, 1954
BsrI	ACTGG	2 cut(s)	209, 1084
BssECI	CCNNGG	6 cut(s)	144, 178, 192, 459, 627, 1561
BssNI	GRCGYC	1 cut(s)	849
BssSI	CACGAG	1 cut(s)	1266
BssT1I	CCWWGG	5 cut(s)	144, 178, 192, 459, 627
Bst2BI	CACGAG	1 cut(s)	1266
Bst2UI	CCWGG	2 cut(s)	1562, 1722
Bst4CI	ACNGT	6 cut(s)	416, 880, 1018, 2042, 2093, 2108
Bst6I	CTCTTC	2 cut(s)	6, 1857
BstACI	GRCGYC	1 cut(s)	849
BstAPI	GCANNNNNTGC	1 cut(s)	127
BstBI	TTCGAA	3 cut(s)	1372, 1965, 2085
BstC8I	GCNNGC	4 cut(s)	289, 1103, 1797, 2266
BstDEI	CTNAG	3 cut(s)	967, 1535, 1893
BstDSI	CCRYGG	2 cut(s)	178, 627
BstF5I	GGATG	1 cut(s)	1436
BstFNI	CGCG	1 cut(s)	1583
BstH2I	RGCGCY	1 cut(s)	1870
BstHHI	GCGC	1 cut(s)	1869
BstMAI	GTCTC	1 cut(s)	1828
BstNI	CCWGG	2 cut(s)	1562, 1722
BstNSI	RCATGY	1 cut(s)	664
BstPAI	GACNNNNGTC	1 cut(s)	881
BstSCI	CCNGG	2 cut(s)	1560, 1720
BstSFI	CTRYAG	1 cut(s)	33
BstSLI	GKGCMC	2 cut(s)	1046, 1284
BstUI	CGCG	1 cut(s)	1583
BstV1I	GCAGC	4 cut(s)	1199, 1771, 2149, 2239
BstV2I	GAAGAC	2 cut(s)	1478, 2094
BstX2I	RGATCY	1 cut(s)	1897
BstYI	RGATCY	1 cut(s)	1897
BsuI	GTATCC	1 cut(s)	2050
BsuRI	GGCC	4 cut(s)	183, 404, 1162, 1725
BtgI	CCRYGG	2 cut(s)	178, 627
BtgZI	GCGATG	1 cut(s)	333
BtrI	CACGTC	1 cut(s)	1003
BtsCI	GGATG	1 cut(s)	1436
BtsI	GCAGTG	2 cut(s)	1377, 1416
BtsIMutI	CAGTG	4 cut(s)	1077, 1377, 1416, 1548
Cac8I	GCNNGC	4 cut(s)	289, 1103, 1797, 2266
CaiI	CAGNNNCTG	1 cut(s)	983
CciI	TCATGA	4 cut(s)	76, 1455, 1461, 2152
CfoI	GCGC	1 cut(s)	1869
Cfr13I	GGNCC	2 cut(s)	148, 402
CseI	GACGC	3 cut(s)	838, 947, 1435
Csp6I	GTAC	6 cut(s)	264, 621, 1019, 1642, 1933, 2094
CspCI	CAANNNNNGTGG	2 cut(s)	598, 633
CviQI	GTAC	6 cut(s)	264, 621, 1019, 1642, 1933, 2094
DdeI	CTNAG	3 cut(s)	967, 1535, 1893
DriI	GACNNNNNGTC	3 cut(s)	154, 477, 717
EaeI	YGGCCR	1 cut(s)	181
Eam1104I	CTCTTC	2 cut(s)	6, 1857
Eam1105I	GACNNNNNGTC	3 cut(s)	154, 477, 717
EarI	CTCTTC	2 cut(s)	6, 1857
EciI	GGCGGA	2 cut(s)	1581, 1752
Eco130I	CCWWGG	5 cut(s)	144, 178, 192, 459, 627
Eco32I	GATATC	3 cut(s)	735, 1571, 2019
Eco47I	GGWCC	1 cut(s)	148
Eco47III	AGCGCT	1 cut(s)	1868
Eco57I	CTGAAG	2 cut(s)	904, 1005
Eco88I	CYCGRG	1 cut(s)	152
EcoRI	GAATTC	1 cut(s)	2081
EcoRII	CCWGG	2 cut(s)	1560, 1720
EcoRV	GATATC	3 cut(s)	735, 1571, 2019
EcoT14I	CCWWGG	5 cut(s)	144, 178, 192, 459, 627
EcoT22I	ATGCAT	1 cut(s)	1399
ErhI	CCWWGG	5 cut(s)	144, 178, 192, 459, 627
FaqI	GGGAC	4 cut(s)	269, 436, 1020, 1234
FauI	CCCGC	1 cut(s)	2205
FauNDI	CATATG	2 cut(s)	1399, 1498
FbaI	TGATCA	3 cut(s)	70, 1458, 2149
FblI	GTMKAC	1 cut(s)	852
Fnu4HI	GCNGC	4 cut(s)	1188, 1785, 2138, 2253
FokI	GGATG	1 cut(s)	1423
Fsp4HI	GCNGC	4 cut(s)	1188, 1785, 2138, 2253
FspBI	CTAG	4 cut(s)	552, 1200, 1445, 1923
GlaI	GCGC	1 cut(s)	1868
GluI	GCNGC	4 cut(s)	1188, 1785, 2138, 2253
HaeII	RGCGCY	1 cut(s)	1870
HaeIII	GGCC	4 cut(s)	183, 404, 1162, 1725
HapII	CCGG	2 cut(s)	1302, 1319
HgaI	GACGC	3 cut(s)	838, 947, 1435
HhaI	GCGC	1 cut(s)	1869
Hin1I	GRCGYC	1 cut(s)	849
Hin6I	GCGC	1 cut(s)	1867
HinP1I	GCGC	1 cut(s)	1867
HincII	GTYRAC	1 cut(s)	482
HindII	GTYRAC	1 cut(s)	482
HindIII	AAGCTT	1 cut(s)	987
HinfI	GANTC	6 cut(s)	140, 155, 472, 478, 687, 1356
HpaII	CCGG	2 cut(s)	1302, 1319
HphI	GGTGA	5 cut(s)	223, 261, 289, 352, 428
Hpy166II	GTNNAC	7 cut(s)	482, 828, 853, 1044, 1282, 1327, 1935
Hpy8I	GTNNAC	7 cut(s)	482, 828, 853, 1044, 1282, 1327, 1935
Hpy99I	CGWCG	2 cut(s)	880, 1429
HpyAV	CCTTC	6 cut(s)	304, 1138, 1343, 1450, 1961, 1972
HpyCH4III	ACNGT	6 cut(s)	416, 880, 1018, 2042, 2093, 2108
HpyCH4IV	ACGT	2 cut(s)	425, 1002
HpyF3I	CTNAG	3 cut(s)	967, 1535, 1893
HpySE526I	ACGT	2 cut(s)	425, 1002
Hsp92I	GRCGYC	1 cut(s)	849
HspAI	GCGC	1 cut(s)	1867
Kpn2I	TCCGGA	1 cut(s)	1318
Ksp22I	TGATCA	3 cut(s)	70, 1458, 2149
LguI	GCTCTTC	1 cut(s)	1857
LmnI	GCTCC	2 cut(s)	178, 2261
Lsp1109I	GCAGC	4 cut(s)	1199, 1771, 2149, 2239
LweI	GCATC	6 cut(s)	91, 315, 557, 1639, 1690, 1904
MaeI	CTAG	4 cut(s)	552, 1200, 1445, 1923
MaeII	ACGT	2 cut(s)	425, 1002
MaeIII	GTNAC	4 cut(s)	156, 1357, 1420, 2245
MflI	RGATCY	1 cut(s)	1897
MhlI	GDGCHC	4 cut(s)	293, 1046, 1284, 1548
MlsI	TGGCCA	1 cut(s)	183
MluCI	AATT	9 cut(s)	509, 590, 810, 843, 1047, 1665, 1689, 2008, 2081
MluNI	TGGCCA	1 cut(s)	183
MlyI	GAGTC	5 cut(s)	134, 164, 466, 487, 1365
MmeI	TCCRAC	6 cut(s)	661, 899, 1261, 1333, 2046, 2154
Mox20I	TGGCCA	1 cut(s)	183
Mph1103I	ATGCAT	1 cut(s)	1399
MroI	TCCGGA	1 cut(s)	1318
MroXI	GAANNNNTTC	3 cut(s)	1749, 1907, 1994
MscI	TGGCCA	1 cut(s)	183
MseI	TTAA	4 cut(s)	365, 378, 389, 558
MslI	CAYNNNNRTG	4 cut(s)	183, 318, 1255, 1460
Msp20I	TGGCCA	1 cut(s)	183
MspI	CCGG	2 cut(s)	1302, 1319
MspR9I	CCNGG	2 cut(s)	1562, 1722
Mva1269I	GAATGC	1 cut(s)	1758
MvaI	CCWGG	2 cut(s)	1562, 1722
MvnI	CGCG	1 cut(s)	1583
NcoI	CCATGG	2 cut(s)	178, 627
NdeI	CATATG	2 cut(s)	1399, 1498
NlaIV	GGNNCC	1 cut(s)	1854
NmuCI	GTSAC	3 cut(s)	156, 1357, 2245
NsiI	ATGCAT	1 cut(s)	1399
NspI	RCATGY	1 cut(s)	664
NspV	TTCGAA	3 cut(s)	1372, 1965, 2085
PaeR7I	CTCGAG	1 cut(s)	152
PagI	TCATGA	4 cut(s)	76, 1455, 1461, 2152
PciSI	GCTCTTC	1 cut(s)	1857
PcsI	WCGNNNNNNNCGW	1 cut(s)	1962
PctI	GAATGC	1 cut(s)	1758
PdmI	GAANNNNTTC	3 cut(s)	1749, 1907, 1994
PfeI	GAWTC	1 cut(s)	687
PkrI	GCNGC	4 cut(s)	1189, 1786, 2139, 2254
PleI	GAGTC	5 cut(s)	134, 163, 466, 486, 1364
PpsI	GAGTC	5 cut(s)	134, 163, 466, 486, 1364
PshAI	GACNNNNGTC	1 cut(s)	881
PsiI	TTATAA	1 cut(s)	1607
Psp6I	CCWGG	2 cut(s)	1560, 1720
PspGI	CCWGG	2 cut(s)	1560, 1720
PspN4I	GGNNCC	1 cut(s)	1854
PspPI	GGNCC	2 cut(s)	148, 402
PspXI	VCTCGAGB	1 cut(s)	152
PstNI	CAGNNNCTG	1 cut(s)	983
PsuI	RGATCY	1 cut(s)	1897
RsaI	GTAC	6 cut(s)	265, 622, 1020, 1643, 1934, 2095
RsaNI	GTAC	6 cut(s)	264, 621, 1019, 1642, 1933, 2094
RseI	CAYNNNNRTG	4 cut(s)	183, 318, 1255, 1460
SapI	GCTCTTC	1 cut(s)	1857
SaqAI	TTAA	4 cut(s)	365, 378, 389, 558
SatI	GCNGC	4 cut(s)	1188, 1785, 2138, 2253
Sau96I	GGNCC	2 cut(s)	148, 402
ScaI	AGTACT	2 cut(s)	1643, 2095
SchI	GAGTC	5 cut(s)	134, 164, 466, 487, 1365
ScrFI	CCNGG	2 cut(s)	1562, 1722
SduI	GDGCHC	4 cut(s)	293, 1046, 1284, 1548
SfaNI	GCATC	6 cut(s)	91, 315, 557, 1639, 1690, 1904
SfcI	CTRYAG	1 cut(s)	33
Sfr274I	CTCGAG	1 cut(s)	152
SfuI	TTCGAA	3 cut(s)	1372, 1965, 2085
SinI	GGWCC	1 cut(s)	148
SlaI	CTCGAG	1 cut(s)	152
SmiMI	CAYNNNNRTG	4 cut(s)	183, 318, 1255, 1460
SmlI	CTYRAG	3 cut(s)	152, 518, 1682
SmoI	CTYRAG	3 cut(s)	152, 518, 1682
Sse9I	AATT	9 cut(s)	509, 590, 810, 843, 1047, 1665, 1689, 2008, 2081
SsiI	CCGC	4 cut(s)	200, 1566, 1763, 2212
SspI	AATATT	1 cut(s)	2032
SspMI	CTAG	4 cut(s)	552, 1200, 1445, 1923
StyD4I	CCNGG	2 cut(s)	1560, 1720
StyI	CCWWGG	5 cut(s)	144, 178, 192, 459, 627
TaaI	ACNGT	6 cut(s)	416, 880, 1018, 2042, 2093, 2108
TaiI	ACGT	2 cut(s)	428, 1005
TaqI	TCGA	8 cut(s)	153, 451, 864, 1372, 1633, 1965, 2073, 2085
TasI	AATT	9 cut(s)	509, 590, 810, 843, 1047, 1665, 1689, 2008, 2081
TatI	WGTACW	3 cut(s)	1018, 1641, 2093
TfiI	GAWTC	1 cut(s)	687
Tru1I	TTAA	4 cut(s)	365, 378, 389, 558
Tru9I	TTAA	4 cut(s)	365, 378, 389, 558
TscAI	CASTG	4 cut(s)	1084, 1384, 1416, 1548
TseFI	GTSAC	3 cut(s)	156, 1357, 2245
TseI	GCWGC	4 cut(s)	1187, 1784, 2137, 2252
Tsp45I	GTSAC	3 cut(s)	156, 1357, 2245
TspDTI	ATGAA	8 cut(s)	24, 105, 555, 751, 850, 1987, 2213, 2256
TspGWI	ACGGA	2 cut(s)	118, 1798
TspRI	CASTG	4 cut(s)	1084, 1384, 1416, 1548
VneI	GTGCAC	2 cut(s)	1042, 1280
VpaK11BI	GGWCC	1 cut(s)	148
XapI	RAATTY	3 cut(s)	509, 1665, 2081
XceI	RCATGY	1 cut(s)	664
XhoI	CTCGAG	1 cut(s)	152
XmiI	GTMKAC	1 cut(s)	852
XmnI	GAANNNNTTC	3 cut(s)	1749, 1907, 1994
XspI	CTAG	4 cut(s)	552, 1200, 1445, 1923
ZrmI	AGTACT	2 cut(s)	1643, 2095
Zsp2I	ATGCAT	1 cut(s)	1399

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.