Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001214

Physical Location & Seq

Genomic Coordinates Reverse (-)

24595181 .. 24596395

Gene Structure 1215 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1136700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1215 bp

ATGTCCGCGGCGACGCCGTCTTACTGGTGCTACAGTTGTAGGCGTTTCGTTAGGGTTTTCAACGACGCCGACGTCTCCACCGCCTCCGCCCTATCCTCCTCAGTGGTGTGCTCGGACTGCGAGAGTGGCTTCGTAGAGGCGATCGACAACCCGACCTCCTCCGGAAACGTGGACTCGCGCCGCAGCAGGAGGTTTTCAGCTGCCTCGATGTACATGATTGGAGCCAACAACCGCTCGGTTTCCAGTCCGGATCATACCGCTTCGGTTCCGTCCGCCCCCCGAAGAGTTAGACGAAACAACGGCGACCGCTCGCCGTTCAATCCGGTAATCGTCCTCCGCGGAACAACGGCCGAAGGCGCCGCCACCACCGAGGAAGGCCGCGGATATGAGCTCTTTTACGACGACGGTAGCGGCTCGGGGCTCCGACCCTTGCCTACAAGCATGTCCGAGTTTCTTCTCGGGTCTAGGTTCGACCGCCTACTTGAACAGCTATCGCAGTTCGAAATCAATGGAATCGGGCGATGCGAGCAGAGACCGGCGTCGAAGTCGGCGATTGAGTCGATGCCGACGATCAAGCTCGATGAGGCTCACATAACGGCGGGGCATCACTGCGCCGTTTGTAAAGAACCGTTCGAAGTTAACACCGAAGCTCAGGAAATGCCCTGCAAGCATATATACCACTCCGATTGTATACTGCCGTGGCTTACGCTCCGGAATTCATGTCCGGTATGCAGGCACGAGCTACCGGCCGATTCGACGCCCGATACGGGTCAGGGTCGAGGTCAGGAAGAGGACGAAAACGCTGGTCTGACGATCTGGAGGCTGCCAGGCGGCGGATTCGCGGTGGGGAGATTTAGTGGGGGGAGAAGAGGCGCCGAGAGAGAGATGCCGGTAGTGTATACGGAGATGGACGGCGGGTTTAGCGGCGGCGGAGCTGCGAGGAGGATTTCGTGGTCAAGAAATGGAGGAAGACGGAGACAGAGCGGTGGGTTGAGGAGATTTTTCATGAATCTGTTTGGTTGTTTCAGCGGGGGTGTGTCGTCGTCGGCAAGTTCGAGTTCGGGTTCGGAGATGAGGGTGGGGACGCCGATGCCGCCACCATCGACGAGTCGTAGAAGAGTGAGGTTTATGGAATTGGAACCGACGGAGACTTCGACGCCGAGGGGGCGGAGGACTTGGTCTATGGAGGTGGATAGCGGGATTGTAAGATGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000209 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009653 GO:0009791 GO:0009826 GO:0009888 GO:0009987 GO:0010026 GO:0010154 GO:0010498 GO:0016043 GO:0016049 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0022412 GO:0022414 GO:0030154 GO:0030163 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032989 GO:0036211 GO:0040007 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0048316 GO:0048468 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0051603 GO:0060560 GO:0061458 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0071840 GO:0090376 GO:0090378 GO:0090558 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1117 zf-RING_2 zinc_ribbon_9

Protein Analysis

405

Amino Acids

44.13

Weight (kDa)

8.47

Isoelectric Point (pI)

75.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zn_ribbon_19	PF14369	7 - 48	2.4e-06	zinc-ribbon
zf-rbx1	PF12678	202 - 245	5.8e-07	RING-H2 zinc finger domain
zf-RING_2	PF13639	203 - 245	1.5e-11	Ring finger domain
zf-C3HC4_2	PF13923	204 - 244	1.8e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	204 - 231	2e-06	RING-like zinc finger
zf-C3HC4	PF00097	204 - 244	3e-06	Zinc finger, C3HC4 type (RING finger)
DUF1117	PF06547	269 - 357	1.2e-36	Protein of unknown function (DUF1117)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001214)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39720 AT2G39720 AT3G46620 AT5G59550 AT5G59550
fragaria_vesca	FvH4_3g36860 FvH4_6g13340 FvH4_7g22850
malus_domestica	MD01G1136700.v1.1 MD03G1087100.v1.1 MD04G1131800.v1.1 MD07G1201700.v1.1 MD10G1074300.v1.1 MD11G1012900.v1.1 MD11G1096200.v1.1 MD12G1144900.v1.1
prunus_persica	Prupe.2G238300_v2.0.a1 Prupe.6G071800_v2.0.a1 Prupe.6G258400_v2.0.a1
pyrus_communis	pycom01g15740 pycom03g06910 pycom04g11990 pycom07g18870 pycom11g08150 pycom12g13930
rosa_chinensis	RchiOBHm_Chr1g0368011 RchiOBHm_Chr3g0466231 RchiOBHm_Chr5g0066071
rosa_laevigata	RLG00000024595 RLG00000027247 RLG00000035839
rosa_multiflora	Rmu_co8242689.1_g000001 Rmu_co8418295.1_g000001 Rmu_sc0000350.1_g000012 Rmu_sc0002406.1_g000005 Rmu_sc0005240.1_g000003 Rmu_sc0005240.1_g000004
rosa_roxburghii	Rroxscaffold_1G00014940 Rroxscaffold_4G00288940 Rroxscaffold_6G00414710
rosa_rugosa	Rorug01G0338400 Rorug03G0080500 Rorug05G0376200
rosa_samantha	Rh1AG345200 Rh1BG307500 Rh1CG323100 Rh1DG339600 Rh3BG147700 Rh3DG148400 Rh5AG434600 Rh5BG450900 Rh5CG473200 Rh5DG464500
rosa_wichuraiana	Rw1G030720 Rw3G011940 Rw5G040700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	71
AatII	GACGTC	1 cut(s)	75
AccB1I	GGYRCC	2 cut(s)	356, 872
AccBSI	CCGCTC	3 cut(s)	234, 309, 984
AccI	GTMKAC	2 cut(s)	691, 899
AccII	CGCG	5 cut(s)	8, 178, 339, 381, 842
AccIII	TCCGGA	3 cut(s)	161, 247, 711
AclWI	GGATC	1 cut(s)	258
AcoI	YGGCCR	2 cut(s)	348, 747
AcsI	RAATTY	1 cut(s)	715
AcyI	GRCGYC	9 cut(s)	14, 66, 72, 357, 539, 758, 873, 1085, 1157
AfaI	GTAC	1 cut(s)	212
AfiI	CCNNNNNNNGG	2 cut(s)	767, 833
AgsI	TTSAA	3 cut(s)	61, 319, 485
AjnI	CCWGG	1 cut(s)	826
AluBI	AGCT	7 cut(s)	200, 391, 490, 577, 650, 742, 935
AluI	AGCT	7 cut(s)	200, 391, 490, 577, 650, 742, 935
Alw21I	GWGCWC	2 cut(s)	113, 393
Alw26I	GTCTC	4 cut(s)	79, 526, 970, 1142
AlwI	GGATC	1 cut(s)	258
Ama87I	CYCGRG	2 cut(s)	415, 458
Aor13HI	TCCGGA	3 cut(s)	161, 247, 711
AoxI	GGCC	3 cut(s)	348, 376, 747
ApeKI	GCWGC	4 cut(s)	183, 200, 823, 935
ApoI	RAATTY	1 cut(s)	715
ArsI	GACNNNNNNTTYG	2 cut(s)	1135, 1167
AspLEI	GCGC	4 cut(s)	180, 359, 614, 875
AsuII	TTCGAA	2 cut(s)	501, 633
AvaI	CYCGRG	2 cut(s)	415, 458
BanI	GGYRCC	2 cut(s)	356, 872
BanII	GRGCYC	2 cut(s)	393, 423
BauI	CACGAG	1 cut(s)	737
BbsI	GAAGAC	1 cut(s)	976
Bbv12I	GWGCWC	2 cut(s)	113, 393
BbvI	GCAGC	4 cut(s)	187, 195, 810, 922
BccI	CCATC	3 cut(s)	901, 1108, 1203
BceAI	ACGGC	7 cut(s)	298, 316, 363, 599, 612, 682, 928
BciT130I	CCWGG	1 cut(s)	828
BcoDI	GTCTC	4 cut(s)	79, 526, 970, 1142
BfaI	CTAG	1 cut(s)	465
BfmI	CTRYAG	1 cut(s)	31
BfoI	RGCGCY	2 cut(s)	360, 876
BglI	GCCNNNNNGGC	1 cut(s)	1165
Bme1390I	CCNGG	1 cut(s)	828
BmeT110I	CYCGRG	2 cut(s)	415, 458
BmiI	GGNNCC	6 cut(s)	223, 267, 358, 422, 874, 1140
BmrFI	CCNGG	1 cut(s)	828
BmsI	GCATC	5 cut(s)	512, 552, 613, 876, 1080
BoxI	GACNNNNGTC	1 cut(s)	538
BpiI	GAAGAC	1 cut(s)	976
BpmI	CTGGAG	1 cut(s)	838
Bpu10I	CCTNAGC	1 cut(s)	651
Bpu14I	TTCGAA	2 cut(s)	501, 633
BsaHI	GRCGYC	9 cut(s)	14, 66, 72, 357, 539, 758, 873, 1085, 1157
BsaI	GGTCTC	1 cut(s)	526
BsaJI	CCNNGG	6 cut(s)	6, 337, 369, 379, 698, 1160
BsaWI	WCCGGW	5 cut(s)	161, 247, 322, 711, 724
BsaXI	ACNNNNNCTCC	4 cut(s)	140, 170, 841, 871
Bsc4I	CCNNNNNNNGG	2 cut(s)	767, 833
Bse118I	RCCGGY	3 cut(s)	535, 745, 889
Bse1I	ACTGG	2 cut(s)	29, 243
BseAI	TCCGGA	3 cut(s)	161, 247, 711
BseBI	CCWGG	1 cut(s)	828
BseDI	CCNNGG	6 cut(s)	6, 337, 369, 379, 698, 1160
BseLI	CCNNNNNNNGG	2 cut(s)	767, 833
BseMII	CTCAG	2 cut(s)	114, 665
BseNI	ACTGG	2 cut(s)	29, 243
BseRI	GAGGAG	4 cut(s)	88, 148, 955, 1009
BseX3I	CGGCCG	2 cut(s)	348, 747
BseXI	GCAGC	4 cut(s)	187, 195, 810, 922
Bsh1236I	CGCG	5 cut(s)	8, 178, 339, 381, 842
Bsh1285I	CGRYCG	5 cut(s)	144, 307, 351, 475, 750
BshFI	GGCC	3 cut(s)	350, 378, 749
BshNI	GGYRCC	2 cut(s)	356, 872
BsiEI	CGRYCG	5 cut(s)	144, 307, 351, 475, 750
BsiHKAI	GWGCWC	2 cut(s)	113, 393
BsiHKCI	CYCGRG	2 cut(s)	415, 458
BsiSI	CCGG	8 cut(s)	162, 248, 323, 536, 712, 725, 746, 890
BslFI	GGGAC	1 cut(s)	1096
BslI	CCNNNNNNNGG	2 cut(s)	767, 833
BsmAI	GTCTC	4 cut(s)	79, 526, 970, 1142
BsmBI	CGTCTC	1 cut(s)	79
BsmFI	GGGAC	1 cut(s)	1096
BsnI	GGCC	3 cut(s)	350, 378, 749
Bso31I	GGTCTC	1 cut(s)	526
BsoBI	CYCGRG	2 cut(s)	415, 458
Bsp119I	TTCGAA	2 cut(s)	501, 633
Bsp1286I	GDGCHC	3 cut(s)	113, 393, 423
Bsp13I	TCCGGA	3 cut(s)	161, 247, 711
Bsp1407I	TGTACA	1 cut(s)	210
Bsp143I	GATC	4 cut(s)	141, 250, 570, 813
BspANI	GGCC	3 cut(s)	350, 378, 749
BspCNI	CTCAG	2 cut(s)	113, 664
BspEI	TCCGGA	3 cut(s)	161, 247, 711
BspFNI	CGCG	5 cut(s)	8, 178, 339, 381, 842
BspHI	TCATGA	1 cut(s)	1005
BspLI	GGNNCC	6 cut(s)	223, 267, 358, 422, 874, 1140
BspPI	GGATC	1 cut(s)	258
BspT104I	TTCGAA	2 cut(s)	501, 633
BspT107I	GGYRCC	2 cut(s)	356, 872
BspTNI	GGTCTC	1 cut(s)	526
BsrBI	CCGCTC	3 cut(s)	234, 309, 984
BsrFI	RCCGGY	3 cut(s)	535, 745, 889
BsrGI	TGTACA	1 cut(s)	210
BsrI	ACTGG	2 cut(s)	29, 243
BssAI	RCCGGY	3 cut(s)	535, 745, 889
BssECI	CCNNGG	6 cut(s)	6, 337, 369, 379, 698, 1160
BssMI	GATC	4 cut(s)	141, 250, 570, 813
BssNAI	GTATAC	2 cut(s)	692, 900
BssNI	GRCGYC	9 cut(s)	14, 66, 72, 357, 539, 758, 873, 1085, 1157
BssSI	CACGAG	1 cut(s)	737
Bst1107I	GTATAC	2 cut(s)	692, 900
Bst2BI	CACGAG	1 cut(s)	737
Bst2UI	CCWGG	1 cut(s)	828
Bst4CI	ACNGT	3 cut(s)	35, 407, 630
Bst6I	CTCTTC	4 cut(s)	277, 783, 862, 1111
BstACI	GRCGYC	9 cut(s)	14, 66, 72, 357, 539, 758, 873, 1085, 1157
BstAUI	TGTACA	1 cut(s)	210
BstBI	TTCGAA	2 cut(s)	501, 633
BstC8I	GCNNGC	4 cut(s)	311, 527, 668, 734
BstDEI	CTNAG	2 cut(s)	100, 651
BstDSI	CCRYGG	4 cut(s)	6, 337, 379, 698
BstFNI	CGCG	5 cut(s)	8, 178, 339, 381, 842
BstH2I	RGCGCY	2 cut(s)	360, 876
BstHHI	GCGC	4 cut(s)	180, 359, 614, 875
BstKTI	GATC	4 cut(s)	144, 253, 573, 816
BstMAI	GTCTC	4 cut(s)	79, 526, 970, 1142
BstMBI	GATC	4 cut(s)	141, 250, 570, 813
BstMCI	CGRYCG	5 cut(s)	144, 307, 351, 475, 750
BstMWI	GCNNNNNNNGC	8 cut(s)	117, 126, 356, 526, 667, 921, 1093, 1165
BstNI	CCWGG	1 cut(s)	828
BstNSI	RCATGY	1 cut(s)	445
BstPAI	GACNNNNGTC	1 cut(s)	538
BstSCI	CCNGG	1 cut(s)	826
BstSFI	CTRYAG	1 cut(s)	31
BstUI	CGCG	5 cut(s)	8, 178, 339, 381, 842
BstV1I	GCAGC	4 cut(s)	187, 195, 810, 922
BstV2I	GAAGAC	1 cut(s)	976
BstZ17I	GTATAC	2 cut(s)	692, 900
BstZI	CGGCCG	2 cut(s)	348, 747
BsuRI	GGCC	3 cut(s)	350, 378, 749
BtgI	CCRYGG	4 cut(s)	6, 337, 379, 698
BtgZI	GCGATG	1 cut(s)	535
BtsI	GCAGTG	1 cut(s)	607
BtsIMutI	CAGTG	2 cut(s)	108, 607
Cac8I	GCNNGC	4 cut(s)	311, 527, 668, 734
CciI	TCATGA	1 cut(s)	1005
CfoI	GCGC	4 cut(s)	180, 359, 614, 875
Cfr10I	RCCGGY	3 cut(s)	535, 745, 889
Cfr42I	CCGCGG	3 cut(s)	9, 340, 382
CseI	GACGC	6 cut(s)	22, 74, 528, 766, 1093, 1165
Csp6I	GTAC	1 cut(s)	211
CspCI	CAANNNNNGTGG	2 cut(s)	668, 703
CviAII	CATG	4 cut(s)	214, 442, 720, 1006
CviQI	GTAC	1 cut(s)	211
DdeI	CTNAG	2 cut(s)	100, 651
DinI	GGCGCC	2 cut(s)	358, 874
DpnI	GATC	4 cut(s)	143, 252, 572, 815
DpnII	GATC	4 cut(s)	141, 250, 570, 813
DrdI	GACNNNNNNGTC	1 cut(s)	71
DseDI	GACNNNNNNGTC	1 cut(s)	71
EaeI	YGGCCR	2 cut(s)	348, 747
EagI	CGGCCG	2 cut(s)	348, 747
Eam1104I	CTCTTC	4 cut(s)	277, 783, 862, 1111
EarI	CTCTTC	4 cut(s)	277, 783, 862, 1111
EciI	GGCGGA	5 cut(s)	76, 262, 849, 945, 1183
Ecl136II	GAGCTC	1 cut(s)	391
EclXI	CGGCCG	2 cut(s)	348, 747
Eco24I	GRGCYC	2 cut(s)	393, 423
Eco31I	GGTCTC	1 cut(s)	526
Eco52I	CGGCCG	2 cut(s)	348, 747
Eco53kI	GAGCTC	1 cut(s)	391
Eco88I	CYCGRG	2 cut(s)	415, 458
EcoICRI	GAGCTC	1 cut(s)	391
EcoRI	GAATTC	1 cut(s)	715
EcoRII	CCWGG	1 cut(s)	826
EcoT38I	GRGCYC	2 cut(s)	393, 423
EgeI	GGCGCC	2 cut(s)	358, 874
EheI	GGCGCC	2 cut(s)	358, 874
Esp3I	CGTCTC	1 cut(s)	79
FaeI	CATG	4 cut(s)	217, 445, 723, 1009
FaqI	GGGAC	1 cut(s)	1096
FatI	CATG	4 cut(s)	213, 441, 719, 1005
FauI	CCCGC	4 cut(s)	592, 908, 1022, 1190
FblI	GTMKAC	2 cut(s)	691, 899
FriOI	GRGCYC	2 cut(s)	393, 423
FspBI	CTAG	1 cut(s)	465
GlaI	GCGC	4 cut(s)	179, 358, 613, 874
GsuI	CTGGAG	1 cut(s)	838
HaeII	RGCGCY	2 cut(s)	360, 876
HaeIII	GGCC	3 cut(s)	350, 378, 749
HapII	CCGG	8 cut(s)	162, 248, 323, 536, 712, 725, 746, 890
HgaI	GACGC	6 cut(s)	22, 74, 528, 766, 1093, 1165
HhaI	GCGC	4 cut(s)	180, 359, 614, 875
Hin1I	GRCGYC	9 cut(s)	14, 66, 72, 357, 539, 758, 873, 1085, 1157
Hin1II	CATG	4 cut(s)	217, 445, 723, 1009
Hin6I	GCGC	4 cut(s)	178, 357, 612, 873
HinP1I	GCGC	4 cut(s)	178, 357, 612, 873
HincII	GTYRAC	1 cut(s)	640
HindII	GTYRAC	1 cut(s)	640
HinfI	GANTC	7 cut(s)	173, 513, 557, 752, 837, 1009, 1108
HpaI	GTTAAC	1 cut(s)	640
HpaII	CCGG	8 cut(s)	162, 248, 323, 536, 712, 725, 746, 890
Hpy166II	GTNNAC	4 cut(s)	172, 640, 692, 900
Hpy188I	TCNGA	6 cut(s)	115, 425, 448, 685, 810, 1069
Hpy188III	TCNNGA	8 cut(s)	162, 248, 653, 712, 785, 817, 957, 1006
Hpy8I	GTNNAC	4 cut(s)	172, 640, 692, 900
HpyAV	CCTTC	2 cut(s)	347, 368
HpyCH4III	ACNGT	3 cut(s)	35, 407, 630
HpyCH4IV	ACGT	2 cut(s)	72, 168
HpyCH4V	TGCA	2 cut(s)	666, 732
HpyF10VI	GCNNNNNNNGC	8 cut(s)	117, 126, 356, 526, 667, 921, 1093, 1165
HpyF3I	CTNAG	2 cut(s)	100, 651
HpySE526I	ACGT	2 cut(s)	72, 168
Hsp92I	GRCGYC	9 cut(s)	14, 66, 72, 357, 539, 758, 873, 1085, 1157
Hsp92II	CATG	4 cut(s)	217, 445, 723, 1009
HspAI	GCGC	4 cut(s)	178, 357, 612, 873
KasI	GGCGCC	2 cut(s)	356, 872
Kpn2I	TCCGGA	3 cut(s)	161, 247, 711
KspAI	GTTAAC	1 cut(s)	640
KspI	CCGCGG	3 cut(s)	9, 340, 382
Kzo9I	GATC	4 cut(s)	141, 250, 570, 813
LmnI	GCTCC	4 cut(s)	221, 426, 714, 932
Lsp1109I	GCAGC	4 cut(s)	187, 195, 810, 922
LweI	GCATC	5 cut(s)	512, 552, 613, 876, 1080
MaeI	CTAG	1 cut(s)	465
MaeII	ACGT	2 cut(s)	72, 168
MalI	GATC	4 cut(s)	143, 252, 572, 815
MbiI	CCGCTC	3 cut(s)	234, 309, 984
MboI	GATC	4 cut(s)	141, 250, 570, 813
MboII	GAAGA	6 cut(s)	294, 446, 800, 879, 981, 1128
MhlI	GDGCHC	3 cut(s)	113, 393, 423
MluCI	AATT	2 cut(s)	715, 1133
Mly113I	GGCGCC	2 cut(s)	357, 873
MlyI	GAGTC	3 cut(s)	167, 566, 1117
MmeI	TCCRAC	1 cut(s)	448
MroI	TCCGGA	3 cut(s)	161, 247, 711
MseI	TTAA	1 cut(s)	639
MspA1I	CMGCKG	5 cut(s)	8, 200, 339, 381, 1029
MspI	CCGG	8 cut(s)	162, 248, 323, 536, 712, 725, 746, 890
MspR9I	CCNGG	1 cut(s)	828
MvaI	CCWGG	1 cut(s)	828
MvnI	CGCG	5 cut(s)	8, 178, 339, 381, 842
MwoI	GCNNNNNNNGC	8 cut(s)	117, 126, 356, 526, 667, 921, 1093, 1165
NarI	GGCGCC	2 cut(s)	357, 873
NdeII	GATC	4 cut(s)	141, 250, 570, 813
NlaIII	CATG	4 cut(s)	217, 445, 723, 1009
NlaIV	GGNNCC	6 cut(s)	223, 267, 358, 422, 874, 1140
NmeAIII	GCCGAG	2 cut(s)	901, 1185
NspI	RCATGY	1 cut(s)	445
NspV	TTCGAA	2 cut(s)	501, 633
PagI	TCATGA	1 cut(s)	1005
PcsI	WCGNNNNNNNCGW	6 cut(s)	69, 522, 548, 566, 1052, 1151
PfeI	GAWTC	4 cut(s)	513, 752, 837, 1009
PflFI	GACNNNGTC	2 cut(s)	16, 1177
Ple19I	CGATCG	1 cut(s)	144
PleI	GAGTC	3 cut(s)	167, 565, 1116
PluTI	GGCGCC	2 cut(s)	360, 876
PpsI	GAGTC	3 cut(s)	167, 565, 1116
PshAI	GACNNNNGTC	1 cut(s)	538
Psp124BI	GAGCTC	1 cut(s)	393
Psp6I	CCWGG	1 cut(s)	826
PspGI	CCWGG	1 cut(s)	826
PspN4I	GGNNCC	6 cut(s)	223, 267, 358, 422, 874, 1140
PsyI	GACNNNGTC	2 cut(s)	16, 1177
PvuI	CGATCG	1 cut(s)	144
PvuII	CAGCTG	1 cut(s)	200
RsaI	GTAC	1 cut(s)	212
RsaNI	GTAC	1 cut(s)	211
SacI	GAGCTC	1 cut(s)	393
SacII	CCGCGG	3 cut(s)	9, 340, 382
SaqAI	TTAA	1 cut(s)	639
Sau3AI	GATC	4 cut(s)	141, 250, 570, 813
SchI	GAGTC	3 cut(s)	167, 566, 1117
ScrFI	CCNGG	1 cut(s)	828
SduI	GDGCHC	3 cut(s)	113, 393, 423
SfaNI	GCATC	5 cut(s)	512, 552, 613, 876, 1080
SfcI	CTRYAG	1 cut(s)	31
SfoI	GGCGCC	2 cut(s)	358, 874
Sfr303I	CCGCGG	3 cut(s)	9, 340, 382
SfuI	TTCGAA	2 cut(s)	501, 633
SgrBI	CCGCGG	3 cut(s)	9, 340, 382
Sse9I	AATT	2 cut(s)	715, 1133
SspDI	GGCGCC	2 cut(s)	356, 872
SspMI	CTAG	1 cut(s)	465
SstI	GAGCTC	1 cut(s)	393
StyD4I	CCNGG	1 cut(s)	826
TaaI	ACNGT	3 cut(s)	35, 407, 630
TaiI	ACGT	2 cut(s)	75, 171
TasI	AATT	2 cut(s)	715, 1133
TatI	WGTACW	1 cut(s)	210
TauI	GCSGC	9 cut(s)	11, 183, 362, 381, 414, 834, 927, 930, 1096
TfiI	GAWTC	4 cut(s)	513, 752, 837, 1009
Tru1I	TTAA	1 cut(s)	639
Tru9I	TTAA	1 cut(s)	639
TscAI	CASTG	2 cut(s)	108, 614
TseI	GCWGC	4 cut(s)	183, 200, 823, 935
TspDTI	ATGAA	3 cut(s)	708, 994, 1022
TspGWI	ACGGA	4 cut(s)	258, 917, 988, 1160
TspRI	CASTG	2 cut(s)	108, 614
Tth111I	GACNNNGTC	2 cut(s)	16, 1177
XapI	RAATTY	1 cut(s)	715
XceI	RCATGY	1 cut(s)	445
XmiI	GTMKAC	2 cut(s)	691, 899
XspI	CTAG	1 cut(s)	465
ZraI	GACGTC	1 cut(s)	73

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.