Rmu_sc0000350.1_g000012

E3 ubiquitin-protein ligase RING1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000350.1
OG0001214
Physical Location & Seq
Forward (+)
36538 .. 36978
441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000350.1_g000012.1.cds

Sequence Viewer

Length: 441 bp
ctgccgagtgacgtacgcggcggcggtgggagcggcaggccctcactggagactgctctgatcggagaggaagagactgtgggattgactatatggaggttaccgggaggcggattcgctgtggggaggttcaccggaggaagaagagcggcggagcgtgagcttccggtggtgtacactgagatggacggtgggttcaacactgctggtggtgctccgaggatgatttcgttggcttcgagtggaagaagaacaagggagagtggtggttttgctagagcttttcgaagtttcttctcctttttcgggaggcttaggagctcatcttcaaggtcgagctcggagtctcgtttagcaagaagaagtcggagcagtagtgtgttcagtagatcttcacggcggcggaatcagggctgggcttttgaagatgatgagaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1117 zf-RING_2 zinc_ribbon_9

Protein Analysis

146

Amino Acids

15.81

Weight (kDa)

11.62

Isoelectric Point (pI)

91.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 33, 149
AccII CGCG 1 cut(s) 18
AciI CCGC 9 cut(s) 18, 21, 24, 33, 111, 149, 152, 400, 403
AfaI GTAC 2 cut(s) 15, 176
AfiI CCNNNNNNNGG 2 cut(s) 110, 306
AgsI TTSAA 3 cut(s) 199, 330, 425
AloI GAACNNNNNNTCC 2 cut(s) 179, 211
AluBI AGCT 4 cut(s) 163, 281, 321, 339
AluI AGCT 4 cut(s) 163, 281, 321, 339
Alw21I GWGCWC 3 cut(s) 217, 323, 341
Alw26I GTCTC 3 cut(s) 44, 68, 351
AoxI GGCC 1 cut(s) 38
AspS9I GGNCC 1 cut(s) 39
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 1 cut(s) 124
AsuII TTCGAA 1 cut(s) 286
BanII GRGCYC 2 cut(s) 323, 341
Bbv12I GWGCWC 3 cut(s) 217, 323, 341
BccI CCATC 1 cut(s) 178
BceAI ACGGC 1 cut(s) 413
BcnI CCSGG 1 cut(s) 105
BcoDI GTCTC 3 cut(s) 44, 68, 351
BfaI CTAG 1 cut(s) 276
BglII AGATCT 1 cut(s) 389
BisI GCNGC 5 cut(s) 19, 22, 34, 150, 401
BlsI GCNGC 5 cut(s) 20, 23, 35, 151, 402
Bme1390I CCNGG 1 cut(s) 105
BmgT120I GGNCC 1 cut(s) 39
BmrFI CCNGG 1 cut(s) 105
BpmI CTGGAG 1 cut(s) 68
Bpu10I CCTNAGC 1 cut(s) 314
Bpu14I TTCGAA 1 cut(s) 286
BpuMI CCSGG 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 218
BsaWI WCCGGW 2 cut(s) 134, 166
BsaXI ACNNNNNCTCC 2 cut(s) 361, 391
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 306
Bse1I ACTGG 1 cut(s) 51
BseDI CCNNGG 1 cut(s) 218
BseGI GGATG 1 cut(s) 228
BseLI CCNNNNNNNGG 2 cut(s) 110, 306
BseMII CTCAG 1 cut(s) 171
BseNI ACTGG 1 cut(s) 51
BseYI CCCAGC 1 cut(s) 414
Bsh1236I CGCG 1 cut(s) 18
BshFI GGCC 1 cut(s) 40
BsiHKAI GWGCWC 3 cut(s) 217, 323, 341
BsiSI CCGG 3 cut(s) 104, 135, 167
BsiWI CGTACG 1 cut(s) 13
BslI CCNNNNNNNGG 2 cut(s) 110, 306
BsmAI GTCTC 3 cut(s) 44, 68, 351
BsnI GGCC 1 cut(s) 40
Bsp119I TTCGAA 1 cut(s) 286
Bsp1286I GDGCHC 3 cut(s) 217, 323, 341
Bsp1407I TGTACA 1 cut(s) 174
Bsp143I GATC 2 cut(s) 60, 389
BspACI CCGC 9 cut(s) 18, 21, 24, 33, 111, 149, 152, 400, 403
BspANI GGCC 1 cut(s) 40
BspCNI CTCAG 1 cut(s) 172
BspFNI CGCG 1 cut(s) 18
BspQI GCTCTTC 1 cut(s) 139
BspT104I TTCGAA 1 cut(s) 286
BsrBI CCGCTC 2 cut(s) 33, 149
BsrGI TGTACA 1 cut(s) 174
BsrI ACTGG 1 cut(s) 51
BssECI CCNNGG 1 cut(s) 218
BssMI GATC 2 cut(s) 60, 389
Bst4CI ACNGT 2 cut(s) 79, 191
Bst6I CTCTTC 2 cut(s) 66, 139
BstAUI TGTACA 1 cut(s) 174
BstBI TTCGAA 1 cut(s) 286
BstC8I GCNNGC 1 cut(s) 38
BstDEI CTNAG 2 cut(s) 180, 314
BstEII GGTNACC 1 cut(s) 99
BstF5I GGATG 1 cut(s) 228
BstFNI CGCG 1 cut(s) 18
BstKTI GATC 2 cut(s) 63, 392
BstMAI GTCTC 3 cut(s) 44, 68, 351
BstMBI GATC 2 cut(s) 60, 389
BstMWI GCNNNNNNNGC 2 cut(s) 30, 212
BstPI GGTNACC 1 cut(s) 99
BstSCI CCNGG 1 cut(s) 103
BstUI CGCG 1 cut(s) 18
BstX2I RGATCY 1 cut(s) 389
BstYI RGATCY 1 cut(s) 389
BsuRI GGCC 1 cut(s) 40
BtsCI GGATG 1 cut(s) 228
BtsI GCAGTG 1 cut(s) 201
BtsIMutI CAGTG 3 cut(s) 44, 177, 201
Cac8I GCNNGC 1 cut(s) 38
Cfr13I GGNCC 1 cut(s) 39
Csp6I GTAC 2 cut(s) 14, 175
CviJI RGCY 9 cut(s) 40, 163, 236, 281, 313, 321, 339, 414, 419
CviKI_1 RGCY 9 cut(s) 40, 163, 236, 281, 313, 321, 339, 414, 419
CviQI GTAC 2 cut(s) 14, 175
DdeI CTNAG 2 cut(s) 180, 314
DpnI GATC 2 cut(s) 62, 391
DpnII GATC 2 cut(s) 60, 389
Eam1104I CTCTTC 2 cut(s) 66, 139
EarI CTCTTC 2 cut(s) 66, 139
EciI GGCGGA 3 cut(s) 126, 167, 418
Ecl136II GAGCTC 2 cut(s) 321, 339
Eco24I GRGCYC 2 cut(s) 323, 341
Eco53kI GAGCTC 2 cut(s) 321, 339
Eco91I GGTNACC 1 cut(s) 99
EcoICRI GAGCTC 2 cut(s) 321, 339
EcoO109I RGGNCCY 1 cut(s) 39
EcoO65I GGTNACC 1 cut(s) 99
EcoT38I GRGCYC 2 cut(s) 323, 341
FaiI YATR 2 cut(s) 92, 94
Fnu4HI GCNGC 5 cut(s) 19, 22, 34, 150, 401
FokI GGATG 1 cut(s) 235
FriOI GRGCYC 2 cut(s) 323, 341
Fsp4HI GCNGC 5 cut(s) 19, 22, 34, 150, 401
FspBI CTAG 1 cut(s) 276
GluI GCNGC 5 cut(s) 19, 22, 34, 150, 401
GsaI CCCAGC 1 cut(s) 418
GsuI CTGGAG 1 cut(s) 68
HaeIII GGCC 1 cut(s) 40
HapII CCGG 3 cut(s) 104, 135, 167
HinfI GANTC 3 cut(s) 114, 344, 406
HpaII CCGG 3 cut(s) 104, 135, 167
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 3 cut(s) 132, 175, 177
Hpy188I TCNGA 5 cut(s) 60, 65, 219, 343, 369
Hpy188III TCNNGA 1 cut(s) 307
Hpy8I GTNNAC 3 cut(s) 132, 175, 177
HpyCH4III ACNGT 2 cut(s) 79, 191
HpyCH4IV ACGT 1 cut(s) 12
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 212
HpyF3I CTNAG 2 cut(s) 180, 314
HpySE526I ACGT 1 cut(s) 12
Kzo9I GATC 2 cut(s) 60, 389
LguI GCTCTTC 1 cut(s) 139
LmnI GCTCC 5 cut(s) 30, 154, 220, 318, 369
LpnPI CCDG 8 cut(s) 22, 32, 117, 148, 180, 192, 395, 400
MaeI CTAG 1 cut(s) 276
MaeII ACGT 1 cut(s) 12
MaeIII GTNAC 2 cut(s) 8, 99
MalI GATC 2 cut(s) 62, 391
MbiI CCGCTC 2 cut(s) 33, 149
MboI GATC 2 cut(s) 60, 389
MflI RGATCY 1 cut(s) 389
MhlI GDGCHC 3 cut(s) 217, 323, 341
MlyI GAGTC 1 cut(s) 353
MmeI TCCRAC 1 cut(s) 347
MnlI CCTC 8 cut(s) 52, 61, 90, 101, 120, 131, 213, 303
MslI CAYNNNNRTG 1 cut(s) 182
MspI CCGG 3 cut(s) 104, 135, 167
MspR9I CCNGG 1 cut(s) 105
MvnI CGCG 1 cut(s) 18
MwoI GCNNNNNNNGC 2 cut(s) 30, 212
NciI CCSGG 1 cut(s) 105
NdeII GATC 2 cut(s) 60, 389
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 8
NspV TTCGAA 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 139
PcsI WCGNNNNNNNCGW 1 cut(s) 236
PfeI GAWTC 2 cut(s) 114, 406
Pfl23II CGTACG 1 cut(s) 13
PkrI GCNGC 5 cut(s) 20, 23, 35, 151, 402
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
Psp124BI GAGCTC 2 cut(s) 323, 341
PspEI GGTNACC 1 cut(s) 99
PspFI CCCAGC 1 cut(s) 414
PspLI CGTACG 1 cut(s) 13
PspPI GGNCC 1 cut(s) 39
PsuI RGATCY 1 cut(s) 389
RsaI GTAC 2 cut(s) 15, 176
RsaNI GTAC 2 cut(s) 14, 175
RseI CAYNNNNRTG 1 cut(s) 182
SacI GAGCTC 2 cut(s) 323, 341
SapI GCTCTTC 1 cut(s) 139
SatI GCNGC 5 cut(s) 19, 22, 34, 150, 401
Sau3AI GATC 2 cut(s) 60, 389
Sau96I GGNCC 1 cut(s) 39
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 3 cut(s) 217, 323, 341
SetI ASST 8 cut(s) 15, 101, 131, 165, 283, 323, 335, 341
SfuI TTCGAA 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 182
SsiI CCGC 9 cut(s) 18, 21, 24, 33, 111, 149, 152, 400, 403
SspMI CTAG 1 cut(s) 276
SstI GAGCTC 2 cut(s) 323, 341
StyD4I CCNGG 1 cut(s) 103
TaaI ACNGT 2 cut(s) 79, 191
TaiI ACGT 1 cut(s) 15
TaqI TCGA 3 cut(s) 239, 286, 335
TatI WGTACW 1 cut(s) 174
TauI GCSGC 5 cut(s) 21, 24, 36, 152, 403
TfiI GAWTC 2 cut(s) 114, 406
TscAI CASTG 3 cut(s) 51, 184, 208
TseFI GTSAC 1 cut(s) 8
Tsp45I GTSAC 1 cut(s) 8
TspRI CASTG 3 cut(s) 51, 184, 208
XspI CTAG 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.