MD12G1144900.v1.1

E3 ubiquitin-protein ligase RING1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001214
Physical Location & Seq
Reverse (-)
22395846 .. 22396919
1074 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1144900.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGGCTTCCATGGCCGCATCGTCCTACTGGTGCTACGCGTGTAGCCGGTCGATTCATGTTCGGGTTCGGACCCAAGATTCGATCCTCTGCCCCGATTGCGGAGCTGGGTTTATCGAAGAGATCCCGACCCCGACCCCGACCCGATCGTCTCCGGTCCACCACCCGTTTCCCACCGCCGCGATGTTCCTCGACAGCGCCCCGGCTTCGCAGAGTCCGAGCCCGCCTATACTTCGCCGGAATCAGAGAAGCGGCCGCGATCGGTCCTCGTTTAACCCGGTCATCGTTCTCCGGGGCCCACCTGACACAGAATCCGACCCGGGAAGTTTCGAGCTGTTCTATGACGATGGAGCCGGGATGGGTCTCCGCCCGCTGCCGTCGTCGATGTCGGATATCTTAATGGGTTCGGGTTTCGACCGGTTGCTCCACCAGTTGACCCAATTGGAAAACGGTGTCGCGCGGCTCGACCACCCTCCGGCGTCCAAAGCCGCGATCGAGTCGATGCCCGTCATCAAAATCGCCGACAGCCACGTCTCGACGGAGTCGCACTGCGCCGTTTGCAAAGAGGCTTTTGAGCTCGACTCTGAAGCTCGGGAGATGCCGTGTAAGCACATCTACCACTCAGATTGTATCTTCCCATGGCTTTCGATCCGAAATTCGTGCCCGGTTTGCCGGCACCAGCTCCCCACCGATGTGAGAGGCCCGCGTGGCAGTGGCATGGCTTCGCCAGAGAACAATGGCGTGGTTGGAGAAGAAGAGACAGTGGGGTTGACTATCTGGAGATTGCCTGGTGGCGGATTCGCTGTGGGGAGGTTCACCGGAGGCAGAAGAGCTGCGGAGCGCGAGCTTCCAGTTGTTTACACCGAGATGGATGGTGGGTTCAATACGGCCGGTGCTCCTAGTAGAATTTCATGGGCTTCTAGAAGAAGGGCTAGAGAGAGTGGGGGGTTCGCTCAGGCCTTTCGCAATTTCTTCTCTTTTTTCGGCAGGCTGAGAAGCTCGAGAAGTCGGTCTGGCAATGTGTTCAGTAGACCATCACGGAGGAGGAGCCAGCGTTGGGCTTTGGAAGACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1117 zf-RING_2 zinc_ribbon_9

Protein Analysis

358

Amino Acids

39.22

Weight (kDa)

8.74

Isoelectric Point (pI)

82.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 7 - 41 8.2e-14 zinc-ribbon
zf-rbx1 PF12678 178 - 224 1.5e-06 RING-H2 zinc finger domain
zf-RING_2 PF13639 182 - 224 5.4e-12 Ring finger domain
zf-C3HC4_2 PF13923 183 - 223 3.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 183 - 223 9.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 183 - 210 2.9e-06 RING-like zinc finger
DUF1117 PF06547 254 - 350 1.7e-37 Protein of unknown function (DUF1117)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 145, 527
AccB1I GGYRCC 1 cut(s) 672
AccI GTMKAC 1 cut(s) 1027
AccII CGCG 8 cut(s) 38, 179, 255, 455, 457, 488, 703, 840
AclWI GGATC 3 cut(s) 76, 115, 640
AcoI YGGCCR 3 cut(s) 12, 250, 885
AcsI RAATTY 2 cut(s) 652, 903
AcuI CTGAAG 1 cut(s) 603
AcyI GRCGYC 1 cut(s) 476
AfiI CCNNNNNNNGG 4 cut(s) 98, 472, 791, 1054
AflIII ACRYGT 1 cut(s) 36
AgeI ACCGGT 1 cut(s) 414
AgsI TTSAA 1 cut(s) 880
AjiI CACGTC 1 cut(s) 529
AjnI CCWGG 1 cut(s) 784
AleI CACNNNNGTG 1 cut(s) 689
AloI GAACNNNNNNTCC 2 cut(s) 860, 892
AluBI AGCT 8 cut(s) 104, 331, 574, 587, 679, 830, 844, 996
AluI AGCT 8 cut(s) 104, 331, 574, 587, 679, 830, 844, 996
Alw21I GWGCWC 2 cut(s) 576, 895
Alw26I GTCTC 4 cut(s) 153, 365, 535, 749
AlwI GGATC 3 cut(s) 76, 115, 640
Ama87I CYCGRG 3 cut(s) 316, 588, 997
AoxI GGCC 6 cut(s) 12, 250, 292, 697, 885, 954
ApaI GGGCCC 1 cut(s) 296
ApeKI GCWGC 2 cut(s) 370, 830
ApoI RAATTY 2 cut(s) 652, 903
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
AsiGI ACCGGT 1 cut(s) 414
AspLEI GCGC 4 cut(s) 197, 457, 551, 840
AspS9I GGNCC 6 cut(s) 69, 154, 261, 292, 293, 698
AsuC2I CCSGG 7 cut(s) 200, 275, 290, 317, 318, 352, 662
AsuHPI GGTGA 1 cut(s) 805
AvaI CYCGRG 3 cut(s) 316, 588, 997
AvaII GGWCC 3 cut(s) 69, 154, 261
BaeGI GKGCMC 2 cut(s) 296, 662
BanI GGYRCC 1 cut(s) 672
BanII GRGCYC 3 cut(s) 221, 296, 576
Bbv12I GWGCWC 2 cut(s) 576, 895
BbvI GCAGC 2 cut(s) 357, 817
BccI CCATC 5 cut(s) 338, 349, 859, 863, 1039
BceAI ACGGC 4 cut(s) 358, 536, 583, 900
BcgI CGANNNNNNTGC 2 cut(s) 639, 673
BciT130I CCWGG 1 cut(s) 786
BcnI CCSGG 7 cut(s) 200, 275, 290, 317, 318, 352, 662
BcoDI GTCTC 4 cut(s) 153, 365, 535, 749
BfaI CTAG 3 cut(s) 897, 918, 930
BfoI RGCGCY 1 cut(s) 198
BglI GCCNNNNNGGC 1 cut(s) 705
BisI GCNGC 8 cut(s) 15, 177, 250, 253, 371, 458, 486, 831
BlsI GCNGC 8 cut(s) 16, 178, 251, 254, 372, 459, 487, 832
Bme1390I CCNGG 8 cut(s) 200, 275, 290, 317, 318, 352, 662, 786
Bme18I GGWCC 3 cut(s) 69, 154, 261
BmeT110I CYCGRG 3 cut(s) 316, 588, 997
BmgBI CACGTC 1 cut(s) 529
BmgT120I GGNCC 6 cut(s) 69, 154, 261, 292, 293, 698
BmiI GGNNCC 6 cut(s) 71, 293, 294, 349, 674, 1046
BmrFI CCNGG 8 cut(s) 200, 275, 290, 317, 318, 352, 662, 786
BmsI GCATC 3 cut(s) 26, 489, 585
BplI GAGNNNNNCTC 2 cut(s) 563, 595
BpmI CTGGAG 1 cut(s) 796
Bpu10I CCTNAGC 1 cut(s) 951
BpuMI CCSGG 7 cut(s) 200, 275, 290, 317, 318, 352, 662
BsaHI GRCGYC 1 cut(s) 476
BsaI GGTCTC 1 cut(s) 365
BsaJI CCNNGG 5 cut(s) 9, 198, 289, 316, 635
BsaWI WCCGGW 3 cut(s) 151, 414, 815
BsaXI ACNNNNNCTCC 4 cut(s) 584, 614, 1036, 1066
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 472, 791, 1054
Bse118I RCCGGY 4 cut(s) 45, 414, 669, 887
Bse1I ACTGG 3 cut(s) 32, 427, 848
Bse3DI GCAATG 1 cut(s) 1021
BseBI CCWGG 1 cut(s) 786
BseDI CCNNGG 5 cut(s) 9, 198, 289, 316, 635
BseGI GGATG 2 cut(s) 360, 874
BseLI CCNNNNNNNGG 4 cut(s) 98, 472, 791, 1054
BseMI GCAATG 1 cut(s) 1021
BseMII CTCAG 3 cut(s) 633, 965, 980
BseNI ACTGG 3 cut(s) 32, 427, 848
BseRI GAGGAG 2 cut(s) 1054, 1057
BseSI GKGCMC 2 cut(s) 296, 662
BseX3I CGGCCG 2 cut(s) 250, 885
BseXI GCAGC 2 cut(s) 357, 817
BseYI CCCAGC 1 cut(s) 104
Bsh1236I CGCG 8 cut(s) 38, 179, 255, 455, 457, 488, 703, 840
Bsh1285I CGRYCG 7 cut(s) 50, 146, 253, 259, 415, 492, 888
BshFI GGCC 6 cut(s) 14, 252, 294, 699, 887, 956
BshNI GGYRCC 1 cut(s) 672
BshTI ACCGGT 1 cut(s) 414
BsiEI CGRYCG 7 cut(s) 50, 146, 253, 259, 415, 492, 888
BsiHKAI GWGCWC 2 cut(s) 576, 895
BsiHKCI CYCGRG 3 cut(s) 316, 588, 997
BslI CCNNNNNNNGG 4 cut(s) 98, 472, 791, 1054
BsmAI GTCTC 4 cut(s) 153, 365, 535, 749
BsmBI CGTCTC 2 cut(s) 153, 535
BsnI GGCC 6 cut(s) 14, 252, 294, 699, 887, 956
Bso31I GGTCTC 1 cut(s) 365
BsoBI CYCGRG 3 cut(s) 316, 588, 997
Bsp120I GGGCCC 1 cut(s) 292
Bsp1286I GDGCHC 5 cut(s) 221, 296, 576, 662, 895
Bsp143I GATC 6 cut(s) 81, 120, 143, 256, 489, 645
Bsp19I CCATGG 2 cut(s) 9, 635
BspANI GGCC 6 cut(s) 14, 252, 294, 699, 887, 956
BspCNI CTCAG 3 cut(s) 632, 964, 981
BspFNI CGCG 8 cut(s) 38, 179, 255, 455, 457, 488, 703, 840
BspLI GGNNCC 6 cut(s) 71, 293, 294, 349, 674, 1046
BspPI GGATC 3 cut(s) 76, 115, 640
BspQI GCTCTTC 1 cut(s) 820
BspT107I GGYRCC 1 cut(s) 672
BspTNI GGTCTC 1 cut(s) 365
BsrDI GCAATG 1 cut(s) 1021
BsrFI RCCGGY 4 cut(s) 45, 414, 669, 887
BsrI ACTGG 3 cut(s) 32, 427, 848
BssAI RCCGGY 4 cut(s) 45, 414, 669, 887
BssECI CCNNGG 5 cut(s) 9, 198, 289, 316, 635
BssMI GATC 6 cut(s) 81, 120, 143, 256, 489, 645
BssNI GRCGYC 1 cut(s) 476
BssT1I CCWWGG 2 cut(s) 9, 635
Bst2UI CCWGG 1 cut(s) 786
Bst4CI ACNGT 2 cut(s) 449, 760
Bst6I CTCTTC 3 cut(s) 111, 747, 820
BstACI GRCGYC 1 cut(s) 476
BstC8I GCNNGC 7 cut(s) 221, 368, 671, 701, 842, 986, 1049
BstDEI CTNAG 3 cut(s) 619, 951, 989
BstDSI CCRYGG 2 cut(s) 9, 635
BstF5I GGATG 2 cut(s) 360, 874
BstFNI CGCG 8 cut(s) 38, 179, 255, 455, 457, 488, 703, 840
BstH2I RGCGCY 1 cut(s) 198
BstHHI GCGC 4 cut(s) 197, 457, 551, 840
BstKTI GATC 6 cut(s) 84, 123, 146, 259, 492, 648
BstMAI GTCTC 4 cut(s) 153, 365, 535, 749
BstMBI GATC 6 cut(s) 81, 120, 143, 256, 489, 645
BstMCI CGRYCG 7 cut(s) 50, 146, 253, 259, 415, 492, 888
BstMWI GCNNNNNNNGC 7 cut(s) 11, 96, 482, 555, 604, 666, 705
BstNI CCWGG 1 cut(s) 786
BstSCI CCNGG 8 cut(s) 198, 273, 288, 315, 316, 350, 660, 784
BstSLI GKGCMC 2 cut(s) 296, 662
BstUI CGCG 8 cut(s) 38, 179, 255, 455, 457, 488, 703, 840
BstV1I GCAGC 2 cut(s) 357, 817
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
BstZI CGGCCG 2 cut(s) 250, 885
BsuRI GGCC 6 cut(s) 14, 252, 294, 699, 887, 956
BtgI CCRYGG 2 cut(s) 9, 635
BtgZI GCGATG 1 cut(s) 194
BtrI CACGTC 1 cut(s) 529
BtsCI GGATG 2 cut(s) 360, 874
BtsI GCAGTG 2 cut(s) 544, 715
BtsIMutI CAGTG 3 cut(s) 544, 715, 765
Cac8I GCNNGC 7 cut(s) 221, 368, 671, 701, 842, 986, 1049
CciNI GCGGCCGC 1 cut(s) 250
CfoI GCGC 4 cut(s) 197, 457, 551, 840
Cfr10I RCCGGY 4 cut(s) 45, 414, 669, 887
Cfr13I GGNCC 6 cut(s) 69, 154, 261, 292, 293, 698
Cfr9I CCCGGG 1 cut(s) 316
CseI GACGC 1 cut(s) 465
CspAI ACCGGT 1 cut(s) 414
CspCI CAANNNNNGTGG 2 cut(s) 605, 640
CviAII CATG 5 cut(s) 10, 56, 636, 715, 909
DdeI CTNAG 3 cut(s) 619, 951, 989
DpnI GATC 6 cut(s) 83, 122, 145, 258, 491, 647
DpnII GATC 6 cut(s) 81, 120, 143, 256, 489, 645
DrdI GACNNNNNNGTC 2 cut(s) 145, 527
DseDI GACNNNNNNGTC 2 cut(s) 145, 527
EaeI YGGCCR 3 cut(s) 12, 250, 885
EagI CGGCCG 2 cut(s) 250, 885
Eam1104I CTCTTC 3 cut(s) 111, 747, 820
EarI CTCTTC 3 cut(s) 111, 747, 820
EciI GGCGGA 2 cut(s) 353, 807
Ecl136II GAGCTC 1 cut(s) 574
EclXI CGGCCG 2 cut(s) 250, 885
Eco130I CCWWGG 2 cut(s) 9, 635
Eco147I AGGCCT 1 cut(s) 956
Eco24I GRGCYC 3 cut(s) 221, 296, 576
Eco31I GGTCTC 1 cut(s) 365
Eco32I GATATC 1 cut(s) 391
Eco47I GGWCC 3 cut(s) 69, 154, 261
Eco52I CGGCCG 2 cut(s) 250, 885
Eco53kI GAGCTC 1 cut(s) 574
Eco57I CTGAAG 1 cut(s) 603
Eco88I CYCGRG 3 cut(s) 316, 588, 997
EcoICRI GAGCTC 1 cut(s) 574
EcoO109I RGGNCCY 1 cut(s) 292
EcoRII CCWGG 1 cut(s) 784
EcoRV GATATC 1 cut(s) 391
EcoT14I CCWWGG 2 cut(s) 9, 635
EcoT38I GRGCYC 3 cut(s) 221, 296, 576
ErhI CCWWGG 2 cut(s) 9, 635
Esp3I CGTCTC 2 cut(s) 153, 535
FaeI CATG 5 cut(s) 13, 59, 639, 718, 912
FaiI YATR 7 cut(s) 11, 57, 227, 339, 637, 716, 910
FatI CATG 5 cut(s) 9, 55, 635, 714, 908
FauI CCCGC 3 cut(s) 228, 375, 708
FblI GTMKAC 1 cut(s) 1027
Fnu4HI GCNGC 8 cut(s) 15, 177, 250, 253, 371, 458, 486, 831
FokI GGATG 2 cut(s) 367, 881
FriOI GRGCYC 3 cut(s) 221, 296, 576
Fsp4HI GCNGC 8 cut(s) 15, 177, 250, 253, 371, 458, 486, 831
FspBI CTAG 3 cut(s) 897, 918, 930
GlaI GCGC 4 cut(s) 196, 456, 550, 839
GluI GCNGC 8 cut(s) 15, 177, 250, 253, 371, 458, 486, 831
GsaI CCCAGC 1 cut(s) 108
GsuI CTGGAG 1 cut(s) 796
HaeII RGCGCY 1 cut(s) 198
HaeIII GGCC 6 cut(s) 14, 252, 294, 699, 887, 956
HgaI GACGC 1 cut(s) 465
HhaI GCGC 4 cut(s) 197, 457, 551, 840
Hin1I GRCGYC 1 cut(s) 476
Hin1II CATG 5 cut(s) 13, 59, 639, 718, 912
Hin6I GCGC 4 cut(s) 195, 455, 549, 838
HinP1I GCGC 4 cut(s) 195, 455, 549, 838
HincII GTYRAC 2 cut(s) 432, 768
HindII GTYRAC 2 cut(s) 432, 768
HinfI GANTC 9 cut(s) 52, 77, 211, 238, 308, 494, 539, 578, 795
HphI GGTGA 1 cut(s) 805
Hpy166II GTNNAC 6 cut(s) 157, 432, 768, 813, 856, 1028
Hpy188I TCNGA 8 cut(s) 69, 216, 243, 313, 388, 583, 622, 650
Hpy188III TCNNGA 6 cut(s) 124, 532, 590, 775, 918, 999
Hpy8I GTNNAC 6 cut(s) 157, 432, 768, 813, 856, 1028
Hpy99I CGWCG 3 cut(s) 379, 382, 538
HpyAV CCTTC 1 cut(s) 918
HpyCH4III ACNGT 2 cut(s) 449, 760
HpyCH4IV ACGT 1 cut(s) 528
HpyCH4V TGCA 1 cut(s) 558
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 96, 482, 555, 604, 666, 705
HpyF3I CTNAG 3 cut(s) 619, 951, 989
HpySE526I ACGT 1 cut(s) 528
Hsp92I GRCGYC 1 cut(s) 476
Hsp92II CATG 5 cut(s) 13, 59, 639, 718, 912
HspAI GCGC 4 cut(s) 195, 455, 549, 838
KroI GCCGGC 1 cut(s) 669
KroNI GCCGGC 1 cut(s) 671
Kzo9I GATC 6 cut(s) 81, 120, 143, 256, 489, 645
LguI GCTCTTC 1 cut(s) 820
LmnI GCTCC 7 cut(s) 101, 347, 426, 684, 835, 898, 1044
Lsp1109I GCAGC 2 cut(s) 357, 817
LweI GCATC 3 cut(s) 26, 489, 585
MaeI CTAG 3 cut(s) 897, 918, 930
MaeII ACGT 1 cut(s) 528
MalI GATC 6 cut(s) 83, 122, 145, 258, 491, 647
MboI GATC 6 cut(s) 81, 120, 143, 256, 489, 645
MboII GAAGA 7 cut(s) 128, 622, 761, 764, 837, 933, 961
MfeI CAATTG 1 cut(s) 437
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 5 cut(s) 221, 296, 576, 662, 895
MluCI AATT 4 cut(s) 437, 652, 903, 964
MluI ACGCGT 1 cut(s) 36
MlyI GAGTC 4 cut(s) 220, 503, 548, 572
MmeI TCCRAC 3 cut(s) 336, 366, 724
MroNI GCCGGC 1 cut(s) 669
MseI TTAA 2 cut(s) 270, 395
MslI CAYNNNNRTG 2 cut(s) 689, 863
MspA1I CMGCKG 1 cut(s) 370
MspR9I CCNGG 8 cut(s) 200, 275, 290, 317, 318, 352, 662, 786
MunI CAATTG 1 cut(s) 437
MvaI CCWGG 1 cut(s) 786
MvnI CGCG 8 cut(s) 38, 179, 255, 455, 457, 488, 703, 840
MwoI GCNNNNNNNGC 7 cut(s) 11, 96, 482, 555, 604, 666, 705
NaeI GCCGGC 1 cut(s) 671
NciI CCSGG 7 cut(s) 200, 275, 290, 317, 318, 352, 662
NcoI CCATGG 2 cut(s) 9, 635
NdeII GATC 6 cut(s) 81, 120, 143, 256, 489, 645
NgoMIV GCCGGC 1 cut(s) 669
NlaIII CATG 5 cut(s) 13, 59, 639, 718, 912
NlaIV GGNNCC 6 cut(s) 71, 293, 294, 349, 674, 1046
NotI GCGGCCGC 1 cut(s) 250
OliI CACNNNNGTG 1 cut(s) 689
PaeR7I CTCGAG 1 cut(s) 997
PceI AGGCCT 1 cut(s) 956
PciSI GCTCTTC 1 cut(s) 820
PcsI WCGNNNNNNNCGW 2 cut(s) 212, 459
PdiI GCCGGC 1 cut(s) 671
PfeI GAWTC 5 cut(s) 52, 77, 238, 308, 795
PflFI GACNNNGTC 1 cut(s) 538
PinAI ACCGGT 1 cut(s) 414
PkrI GCNGC 8 cut(s) 16, 178, 251, 254, 372, 459, 487, 832
Ple19I CGATCG 3 cut(s) 146, 259, 492
PleI GAGTC 4 cut(s) 219, 502, 547, 572
PpsI GAGTC 4 cut(s) 219, 502, 547, 572
Psp124BI GAGCTC 1 cut(s) 576
Psp6I CCWGG 1 cut(s) 784
PspFI CCCAGC 1 cut(s) 104
PspGI CCWGG 1 cut(s) 784
PspN4I GGNNCC 6 cut(s) 71, 293, 294, 349, 674, 1046
PspOMI GGGCCC 1 cut(s) 292
PspPI GGNCC 6 cut(s) 69, 154, 261, 292, 293, 698
PsuI RGATCY 1 cut(s) 120
PsyI GACNNNGTC 1 cut(s) 538
PvuI CGATCG 3 cut(s) 146, 259, 492
RseI CAYNNNNRTG 2 cut(s) 689, 863
SacI GAGCTC 1 cut(s) 576
SapI GCTCTTC 1 cut(s) 820
SaqAI TTAA 2 cut(s) 270, 395
SatI GCNGC 8 cut(s) 15, 177, 250, 253, 371, 458, 486, 831
Sau3AI GATC 6 cut(s) 81, 120, 143, 256, 489, 645
Sau96I GGNCC 6 cut(s) 69, 154, 261, 292, 293, 698
SchI GAGTC 4 cut(s) 220, 503, 548, 572
ScrFI CCNGG 8 cut(s) 200, 275, 290, 317, 318, 352, 662, 786
SduI GDGCHC 5 cut(s) 221, 296, 576, 662, 895
SfaNI GCATC 3 cut(s) 26, 489, 585
Sfr274I CTCGAG 1 cut(s) 997
SinI GGWCC 3 cut(s) 69, 154, 261
SlaI CTCGAG 1 cut(s) 997
SmaI CCCGGG 1 cut(s) 318
SmiMI CAYNNNNRTG 2 cut(s) 689, 863
SmlI CTYRAG 1 cut(s) 997
SmoI CTYRAG 1 cut(s) 997
Sse9I AATT 4 cut(s) 437, 652, 903, 964
SseBI AGGCCT 1 cut(s) 956
SspMI CTAG 3 cut(s) 897, 918, 930
SstI GAGCTC 1 cut(s) 576
StuI AGGCCT 1 cut(s) 956
StyD4I CCNGG 8 cut(s) 198, 273, 288, 315, 316, 350, 660, 784
StyI CCWWGG 2 cut(s) 9, 635
TaaI ACNGT 2 cut(s) 449, 760
TaiI ACGT 1 cut(s) 531
TaqII GACCGA 2 cut(s) 249, 996
TasI AATT 4 cut(s) 437, 652, 903, 964
TauI GCSGC 6 cut(s) 17, 179, 252, 255, 460, 488
TfiI GAWTC 5 cut(s) 52, 77, 238, 308, 795
Tru1I TTAA 2 cut(s) 270, 395
Tru9I TTAA 2 cut(s) 270, 395
TscAI CASTG 3 cut(s) 551, 715, 765
TseI GCWGC 2 cut(s) 370, 830
TspDTI ATGAA 2 cut(s) 44, 897
TspGWI ACGGA 2 cut(s) 551, 1051
TspMI CCCGGG 1 cut(s) 316
TspRI CASTG 3 cut(s) 551, 715, 765
Tth111I GACNNNGTC 1 cut(s) 538
VpaK11BI GGWCC 3 cut(s) 69, 154, 261
XapI RAATTY 2 cut(s) 652, 903
XbaI TCTAGA 1 cut(s) 917
XhoI CTCGAG 1 cut(s) 997
XmaI CCCGGG 1 cut(s) 316
XmiI GTMKAC 1 cut(s) 1027
XspI CTAG 3 cut(s) 897, 918, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.