Prupe.6G258400_v2.0.a1

E3 ubiquitin-protein ligase RING1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0001214
Physical Location & Seq
Reverse (-)
25024799 .. 25026873
2075 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G258400.1

Sequence Viewer

Length: 1065 bp
ATGGCTTCCCTGGGCTCCGCCTCATACTGGTGCTACACATGTAGCCGATCGATTCGGGTTCGGATCCGGGCCCAAGACTCGATCCTCTGCCCCGATTGCGGCGCCGGATTCATCGAAGAGATCCAGACCCCGACTCGATCTCCGCTCCACCACCCATTCCCCACCGCCGCGATGTTCCTCGACAACACTCCAATTTCGCAGAGCCCGAGCCCGCCTAATCTCCGCCGGAGCCAGAGAAGCAGCCGCGATCGCTCACCGTTCAACCCGGTTATTGTCCTCCGAGGCCCACCTGACACCGAATCCGACCCTGGAAGCTTCGAGCTTTACTACGATGACGGAGCCGGTTTGGGCCTCCGCCCTCTGCCGTCGTCCATGTCGGACTTCTTAATGGGATCGGGTTTCGACCGGCTGCTCCACCAATTGACCCAATTGGAAAACGGCGTCGCACGGCTCGACCACCCTCCGGCGTCGAAAGCCGCCATCGAGTCCATGCCCGTCATCAAGATCGCCGACGACCACGTCTCCACCGAGTCGCACTGCGCTGTTTGCAAAGAGGCGTTCGAGCTCGACTCGGAAGCTCGCGAGATGCCCTGTAAGCACATCTACCACTCCGACTGTATATTCCCATGGCTTTCGATTCGAAACTCGTGCCCGGTTTGCCGCCACCAGCTCCCTACTGACGTGCGCGGGCCGCGTGGCAGTGGCAGAGCCTCGCCGGAGAATAATGGTGTGGTTGGAGAAGAAGAGACGGTGGGGTTGACTATCTGGAGATTGCCCGGGGGTGGATTCGCTGTGGGCAGGTTCACCGGAGGCAGGAGAGCTGCTGAGCGTGAGCTTCCAGTTGTTTACACTGAGATGGATGGTGGGTTCAATACGGCTGGTGCTCCTAGGAGGATTTCATGGGCTTCTAGAAGGAGAGCGAGGGAGAGTGGTGGGTTTGCTCAGGCTTTTCGCAATTTCTTCTCCTTTTTCGGGAGGTTCAGAAGCTCGAGAAGCGGGTCTGGTAATGTGTTTAGTAGATCATCGAGGAGGAGGAGCCAGCGTTGGGCTTTGGAAGATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1117 zf-RING_2 zinc_ribbon_9

Protein Analysis

355

Amino Acids

39.17

Weight (kDa)

8.88

Isoelectric Point (pI)

76.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 518
Acc36I ACCTGC 1 cut(s) 789
AccB1I GGYRCC 1 cut(s) 101
AccBSI CCGCTC 1 cut(s) 145
AccII CGCG 5 cut(s) 170, 246, 582, 687, 694
AclWI GGATC 5 cut(s) 58, 71, 76, 115, 400
AcyI GRCGYC 3 cut(s) 102, 441, 467
AfiI CCNNNNNNNGG 7 cut(s) 27, 98, 463, 782, 813, 972, 1045
AflIII ACRYGT 1 cut(s) 38
AgsI TTSAA 2 cut(s) 262, 871
AjiI CACGTC 2 cut(s) 520, 682
AjnI CCWGG 2 cut(s) 9, 307
AloI GAACNNNNNNTCC 2 cut(s) 851, 883
AluBI AGCT 8 cut(s) 315, 322, 565, 578, 670, 821, 835, 987
AluI AGCT 8 cut(s) 315, 322, 565, 578, 670, 821, 835, 987
Alw21I GWGCWC 2 cut(s) 567, 886
Alw26I GTCTC 2 cut(s) 526, 740
AlwI GGATC 5 cut(s) 58, 71, 76, 115, 400
Ama87I CYCGRG 3 cut(s) 205, 776, 988
AoxI GGCC 4 cut(s) 69, 283, 349, 689
ApaI GGGCCC 1 cut(s) 73
ApeKI GCWGC 3 cut(s) 240, 409, 821
AsiSI GCGATCGC 1 cut(s) 250
AspA2I CCTAGG 1 cut(s) 887
AspLEI GCGC 3 cut(s) 104, 542, 687
AspS9I GGNCC 5 cut(s) 69, 70, 284, 349, 689
AsuC2I CCSGG 5 cut(s) 68, 266, 653, 777, 778
AsuHPI GGTGA 2 cut(s) 246, 796
AsuII TTCGAA 1 cut(s) 640
AvaI CYCGRG 3 cut(s) 205, 776, 988
AvrII CCTAGG 1 cut(s) 887
BaeGI GKGCMC 2 cut(s) 73, 653
BamHI GGATCC 1 cut(s) 63
BanI GGYRCC 1 cut(s) 101
BanII GRGCYC 5 cut(s) 17, 73, 206, 212, 567
BauI CACGAG 1 cut(s) 646
Bbv12I GWGCWC 2 cut(s) 567, 886
BbvI GCAGC 3 cut(s) 252, 396, 808
BccI CCATC 3 cut(s) 488, 850, 854
BceAI ACGGC 4 cut(s) 349, 454, 464, 891
BcgI CGANNNNNNTGC 2 cut(s) 630, 664
BciT130I CCWGG 2 cut(s) 11, 309
BcnI CCSGG 5 cut(s) 68, 266, 653, 777, 778
BcoDI GTCTC 2 cut(s) 526, 740
BfaI CTAG 2 cut(s) 888, 909
BfoI RGCGCY 1 cut(s) 105
BfuAI ACCTGC 1 cut(s) 789
BisI GCNGC 9 cut(s) 100, 168, 241, 244, 410, 477, 661, 692, 822
BlnI CCTAGG 1 cut(s) 887
BlpI GCTNAGC 1 cut(s) 825
BlsI GCNGC 9 cut(s) 101, 169, 242, 245, 411, 478, 662, 693, 823
Bme1390I CCNGG 7 cut(s) 11, 68, 266, 309, 653, 777, 778
BmeT110I CYCGRG 3 cut(s) 205, 776, 988
BmgBI CACGTC 2 cut(s) 520, 682
BmgT120I GGNCC 5 cut(s) 69, 70, 284, 349, 689
BmiI GGNNCC 7 cut(s) 16, 65, 71, 103, 230, 340, 1037
BmrFI CCNGG 7 cut(s) 11, 68, 266, 309, 653, 777, 778
BmsI GCATC 1 cut(s) 576
BplI GAGNNNNNCTC 2 cut(s) 554, 586
BpmI CTGGAG 1 cut(s) 787
Bpu10I CCTNAGC 1 cut(s) 942
Bpu1102I GCTNAGC 1 cut(s) 825
Bpu14I TTCGAA 1 cut(s) 640
BpuMI CCSGG 5 cut(s) 68, 266, 653, 777, 778
Bsa29I ATCGAT 1 cut(s) 50
BsaHI GRCGYC 3 cut(s) 102, 441, 467
BsaJI CCNNGG 8 cut(s) 9, 10, 280, 307, 626, 776, 777, 887
BsaWI WCCGGW 1 cut(s) 806
BsaXI ACNNNNNCTCC 6 cut(s) 124, 154, 506, 536, 1027, 1057
Bsc4I CCNNNNNNNGG 7 cut(s) 27, 98, 463, 782, 813, 972, 1045
Bse118I RCCGGY 2 cut(s) 341, 405
Bse1I ACTGG 2 cut(s) 32, 839
BseBI CCWGG 2 cut(s) 11, 309
BseCI ATCGAT 1 cut(s) 50
BseDI CCNNGG 8 cut(s) 9, 10, 280, 307, 626, 776, 777, 887
BseGI GGATG 1 cut(s) 865
BseLI CCNNNNNNNGG 7 cut(s) 27, 98, 463, 782, 813, 972, 1045
BseMII CTCAG 3 cut(s) 816, 843, 956
BseNI ACTGG 2 cut(s) 32, 839
BseRI GAGGAG 3 cut(s) 1042, 1045, 1048
BseSI GKGCMC 2 cut(s) 73, 653
BseXI GCAGC 3 cut(s) 252, 396, 808
Bsh1236I CGCG 5 cut(s) 170, 246, 582, 687, 694
Bsh1285I CGRYCG 3 cut(s) 50, 250, 406
BshFI GGCC 4 cut(s) 71, 285, 351, 691
BshNI GGYRCC 1 cut(s) 101
BshVI ATCGAT 1 cut(s) 50
BsiEI CGRYCG 3 cut(s) 50, 250, 406
BsiHKAI GWGCWC 2 cut(s) 567, 886
BsiHKCI CYCGRG 3 cut(s) 205, 776, 988
BslI CCNNNNNNNGG 7 cut(s) 27, 98, 463, 782, 813, 972, 1045
BsmAI GTCTC 2 cut(s) 526, 740
BsmBI CGTCTC 2 cut(s) 526, 740
BsnI GGCC 4 cut(s) 71, 285, 351, 691
BsoBI CYCGRG 3 cut(s) 205, 776, 988
Bsp119I TTCGAA 1 cut(s) 640
Bsp120I GGGCCC 1 cut(s) 69
Bsp1286I GDGCHC 7 cut(s) 17, 73, 206, 212, 567, 653, 886
Bsp143I GATC 9 cut(s) 47, 63, 81, 120, 137, 247, 392, 504, 1019
Bsp1720I GCTNAGC 1 cut(s) 825
Bsp19I CCATGG 1 cut(s) 626
Bsp68I TCGCGA 1 cut(s) 582
BspANI GGCC 4 cut(s) 71, 285, 351, 691
BspCNI CTCAG 3 cut(s) 817, 844, 955
BspDI ATCGAT 1 cut(s) 50
BspFNI CGCG 5 cut(s) 170, 246, 582, 687, 694
BspLI GGNNCC 7 cut(s) 16, 65, 71, 103, 230, 340, 1037
BspMI ACCTGC 1 cut(s) 789
BspPI GGATC 5 cut(s) 58, 71, 76, 115, 400
BspT104I TTCGAA 1 cut(s) 640
BspT107I GGYRCC 1 cut(s) 101
BsrBI CCGCTC 1 cut(s) 145
BsrFI RCCGGY 2 cut(s) 341, 405
BsrI ACTGG 2 cut(s) 32, 839
BssAI RCCGGY 2 cut(s) 341, 405
BssECI CCNNGG 8 cut(s) 9, 10, 280, 307, 626, 776, 777, 887
BssMI GATC 9 cut(s) 47, 63, 81, 120, 137, 247, 392, 504, 1019
BssNI GRCGYC 3 cut(s) 102, 441, 467
BssSI CACGAG 1 cut(s) 646
BssT1I CCWWGG 2 cut(s) 626, 887
Bst2BI CACGAG 1 cut(s) 646
Bst2UI CCWGG 2 cut(s) 11, 309
Bst4CI ACNGT 3 cut(s) 258, 617, 751
Bst6I CTCTTC 2 cut(s) 111, 738
BstACI GRCGYC 3 cut(s) 102, 441, 467
BstBI TTCGAA 1 cut(s) 640
BstC8I GCNNGC 4 cut(s) 212, 580, 689, 1040
BstDEI CTNAG 3 cut(s) 825, 852, 942
BstDSI CCRYGG 1 cut(s) 626
BstF5I GGATG 1 cut(s) 865
BstFNI CGCG 5 cut(s) 170, 246, 582, 687, 694
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 3 cut(s) 104, 542, 687
BstKTI GATC 9 cut(s) 50, 66, 84, 123, 140, 250, 395, 507, 1022
BstMAI GTCTC 2 cut(s) 526, 740
BstMBI GATC 9 cut(s) 47, 63, 81, 120, 137, 247, 392, 504, 1019
BstMCI CGRYCG 3 cut(s) 50, 250, 406
BstMWI GCNNNNNNNGC 9 cut(s) 96, 237, 249, 473, 546, 595, 657, 691, 993
BstNI CCWGG 2 cut(s) 11, 309
BstNSI RCATGY 1 cut(s) 42
BstSCI CCNGG 7 cut(s) 9, 66, 264, 307, 651, 775, 776
BstSLI GKGCMC 2 cut(s) 73, 653
BstUI CGCG 5 cut(s) 170, 246, 582, 687, 694
BstV1I GCAGC 3 cut(s) 252, 396, 808
BstX2I RGATCY 2 cut(s) 63, 120
BstYI RGATCY 2 cut(s) 63, 120
Bsu15I ATCGAT 1 cut(s) 50
BsuRI GGCC 4 cut(s) 71, 285, 351, 691
BsuTUI ATCGAT 1 cut(s) 50
BtgI CCRYGG 1 cut(s) 626
BtgZI GCGATG 1 cut(s) 185
BtrI CACGTC 2 cut(s) 520, 682
BtsCI GGATG 1 cut(s) 865
BtsI GCAGTG 2 cut(s) 535, 706
BtsIMutI CAGTG 3 cut(s) 535, 706, 849
BtuMI TCGCGA 1 cut(s) 582
BveI ACCTGC 1 cut(s) 789
Cac8I GCNNGC 4 cut(s) 212, 580, 689, 1040
CfoI GCGC 3 cut(s) 104, 542, 687
Cfr10I RCCGGY 2 cut(s) 341, 405
Cfr13I GGNCC 5 cut(s) 69, 70, 284, 349, 689
Cfr9I CCCGGG 1 cut(s) 776
ClaI ATCGAT 1 cut(s) 50
CseI GACGC 2 cut(s) 430, 456
CviAII CATG 5 cut(s) 39, 373, 490, 627, 900
DdeI CTNAG 3 cut(s) 825, 852, 942
DinI GGCGCC 1 cut(s) 103
DpnI GATC 9 cut(s) 49, 65, 83, 122, 139, 249, 394, 506, 1021
DpnII GATC 9 cut(s) 47, 63, 81, 120, 137, 247, 392, 504, 1019
DrdI GACNNNNNNGTC 1 cut(s) 518
DseDI GACNNNNNNGTC 1 cut(s) 518
Eam1104I CTCTTC 2 cut(s) 111, 738
EarI CTCTTC 2 cut(s) 111, 738
EciI GGCGGA 3 cut(s) 7, 212, 344
Ecl136II GAGCTC 1 cut(s) 565
Eco130I CCWWGG 2 cut(s) 626, 887
Eco24I GRGCYC 5 cut(s) 17, 73, 206, 212, 567
Eco53kI GAGCTC 1 cut(s) 565
Eco88I CYCGRG 3 cut(s) 205, 776, 988
EcoICRI GAGCTC 1 cut(s) 565
EcoRII CCWGG 2 cut(s) 9, 307
EcoT14I CCWWGG 2 cut(s) 626, 887
EcoT38I GRGCYC 5 cut(s) 17, 73, 206, 212, 567
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
ErhI CCWWGG 2 cut(s) 626, 887
Esp3I CGTCTC 2 cut(s) 526, 740
FaeI CATG 5 cut(s) 42, 376, 493, 630, 903
FaiI YATR 7 cut(s) 25, 40, 374, 491, 620, 628, 901
FatI CATG 5 cut(s) 38, 372, 489, 626, 899
FauI CCCGC 3 cut(s) 219, 680, 989
Fnu4HI GCNGC 9 cut(s) 100, 168, 241, 244, 410, 477, 661, 692, 822
FokI GGATG 1 cut(s) 872
FriOI GRGCYC 5 cut(s) 17, 73, 206, 212, 567
Fsp4HI GCNGC 9 cut(s) 100, 168, 241, 244, 410, 477, 661, 692, 822
FspBI CTAG 2 cut(s) 888, 909
GlaI GCGC 3 cut(s) 103, 541, 686
GluI GCNGC 9 cut(s) 100, 168, 241, 244, 410, 477, 661, 692, 822
GsuI CTGGAG 1 cut(s) 787
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 4 cut(s) 71, 285, 351, 691
HgaI GACGC 2 cut(s) 430, 456
HhaI GCGC 3 cut(s) 104, 542, 687
Hin1I GRCGYC 3 cut(s) 102, 441, 467
Hin1II CATG 5 cut(s) 42, 376, 493, 630, 903
Hin6I GCGC 3 cut(s) 102, 540, 685
HinP1I GCGC 3 cut(s) 102, 540, 685
HincII GTYRAC 1 cut(s) 759
HindII GTYRAC 1 cut(s) 759
HindIII AAGCTT 1 cut(s) 313
HphI GGTGA 2 cut(s) 246, 796
Hpy166II GTNNAC 3 cut(s) 759, 804, 847
Hpy188I TCNGA 7 cut(s) 63, 281, 304, 379, 574, 613, 983
Hpy188III TCNNGA 7 cut(s) 124, 502, 581, 766, 909, 973, 990
Hpy8I GTNNAC 3 cut(s) 759, 804, 847
Hpy99I CGWCG 4 cut(s) 370, 446, 472, 515
HpyAV CCTTC 1 cut(s) 906
HpyCH4III ACNGT 3 cut(s) 258, 617, 751
HpyCH4IV ACGT 2 cut(s) 519, 681
HpyCH4V TGCA 1 cut(s) 549
HpyF10VI GCNNNNNNNGC 9 cut(s) 96, 237, 249, 473, 546, 595, 657, 691, 993
HpyF3I CTNAG 3 cut(s) 825, 852, 942
HpySE526I ACGT 2 cut(s) 519, 681
Hsp92I GRCGYC 3 cut(s) 102, 441, 467
Hsp92II CATG 5 cut(s) 42, 376, 493, 630, 903
HspAI GCGC 3 cut(s) 102, 540, 685
KasI GGCGCC 1 cut(s) 101
Kzo9I GATC 9 cut(s) 47, 63, 81, 120, 137, 247, 392, 504, 1019
LmnI GCTCC 8 cut(s) 20, 150, 228, 338, 417, 675, 889, 1035
Lsp1109I GCAGC 3 cut(s) 252, 396, 808
LweI GCATC 1 cut(s) 576
MaeI CTAG 2 cut(s) 888, 909
MaeII ACGT 2 cut(s) 519, 681
MalI GATC 9 cut(s) 49, 65, 83, 122, 139, 249, 394, 506, 1021
MbiI CCGCTC 1 cut(s) 145
MboI GATC 9 cut(s) 47, 63, 81, 120, 137, 247, 392, 504, 1019
MboII GAAGA 4 cut(s) 128, 752, 755, 952
MfeI CAATTG 2 cut(s) 419, 428
MflI RGATCY 2 cut(s) 63, 120
MhlI GDGCHC 7 cut(s) 17, 73, 206, 212, 567, 653, 886
MluCI AATT 4 cut(s) 192, 419, 428, 955
Mly113I GGCGCC 1 cut(s) 102
MlyI GAGTC 5 cut(s) 71, 127, 494, 539, 563
MmeI TCCRAC 4 cut(s) 327, 357, 636, 715
MseI TTAA 1 cut(s) 386
MslI CAYNNNNRTG 2 cut(s) 28, 854
MspR9I CCNGG 7 cut(s) 11, 68, 266, 309, 653, 777, 778
MunI CAATTG 2 cut(s) 419, 428
MvaI CCWGG 2 cut(s) 11, 309
MvnI CGCG 5 cut(s) 170, 246, 582, 687, 694
MwoI GCNNNNNNNGC 9 cut(s) 96, 237, 249, 473, 546, 595, 657, 691, 993
NarI GGCGCC 1 cut(s) 102
NciI CCSGG 5 cut(s) 68, 266, 653, 777, 778
NcoI CCATGG 1 cut(s) 626
NdeII GATC 9 cut(s) 47, 63, 81, 120, 137, 247, 392, 504, 1019
NlaIII CATG 5 cut(s) 42, 376, 493, 630, 903
NlaIV GGNNCC 7 cut(s) 16, 65, 71, 103, 230, 340, 1037
NruI TCGCGA 1 cut(s) 582
NspI RCATGY 1 cut(s) 42
NspV TTCGAA 1 cut(s) 640
PaeR7I CTCGAG 1 cut(s) 988
PasI CCCWGGG 1 cut(s) 10
PciI ACATGT 1 cut(s) 38
PcsI WCGNNNNNNNCGW 3 cut(s) 203, 450, 525
PfeI GAWTC 5 cut(s) 52, 108, 299, 637, 786
PflFI GACNNNGTC 1 cut(s) 518
PkrI GCNGC 9 cut(s) 101, 169, 242, 245, 411, 478, 662, 693, 823
Ple19I CGATCG 2 cut(s) 50, 250
PleI GAGTC 5 cut(s) 71, 127, 493, 538, 563
PluTI GGCGCC 1 cut(s) 105
PpsI GAGTC 5 cut(s) 71, 127, 493, 538, 563
PscI ACATGT 1 cut(s) 38
Psp124BI GAGCTC 1 cut(s) 567
Psp6I CCWGG 2 cut(s) 9, 307
PspGI CCWGG 2 cut(s) 9, 307
PspN4I GGNNCC 7 cut(s) 16, 65, 71, 103, 230, 340, 1037
PspOMI GGGCCC 1 cut(s) 69
PspPI GGNCC 5 cut(s) 69, 70, 284, 349, 689
PsuI RGATCY 2 cut(s) 63, 120
PsyI GACNNNGTC 1 cut(s) 518
PvuI CGATCG 2 cut(s) 50, 250
RgaI GCGATCGC 1 cut(s) 250
RruI TCGCGA 1 cut(s) 582
RseI CAYNNNNRTG 2 cut(s) 28, 854
SacI GAGCTC 1 cut(s) 567
SaqAI TTAA 1 cut(s) 386
SatI GCNGC 9 cut(s) 100, 168, 241, 244, 410, 477, 661, 692, 822
Sau3AI GATC 9 cut(s) 47, 63, 81, 120, 137, 247, 392, 504, 1019
Sau96I GGNCC 5 cut(s) 69, 70, 284, 349, 689
SchI GAGTC 5 cut(s) 71, 127, 494, 539, 563
ScrFI CCNGG 7 cut(s) 11, 68, 266, 309, 653, 777, 778
SduI GDGCHC 7 cut(s) 17, 73, 206, 212, 567, 653, 886
SfaAI GCGATCGC 1 cut(s) 250
SfaNI GCATC 1 cut(s) 576
SfoI GGCGCC 1 cut(s) 103
Sfr274I CTCGAG 1 cut(s) 988
SfuI TTCGAA 1 cut(s) 640
SgfI GCGATCGC 1 cut(s) 250
SlaI CTCGAG 1 cut(s) 988
SmaI CCCGGG 1 cut(s) 778
SmiMI CAYNNNNRTG 2 cut(s) 28, 854
SmlI CTYRAG 1 cut(s) 988
SmoI CTYRAG 1 cut(s) 988
Sse9I AATT 4 cut(s) 192, 419, 428, 955
SspDI GGCGCC 1 cut(s) 101
SspMI CTAG 2 cut(s) 888, 909
SstI GAGCTC 1 cut(s) 567
StyD4I CCNGG 7 cut(s) 9, 66, 264, 307, 651, 775, 776
StyI CCWWGG 2 cut(s) 626, 887
TaaI ACNGT 3 cut(s) 258, 617, 751
TaiI ACGT 2 cut(s) 522, 684
TasI AATT 4 cut(s) 192, 419, 428, 955
TauI GCSGC 6 cut(s) 102, 170, 246, 479, 663, 694
TfiI GAWTC 5 cut(s) 52, 108, 299, 637, 786
Tru1I TTAA 1 cut(s) 386
Tru9I TTAA 1 cut(s) 386
TscAI CASTG 3 cut(s) 542, 706, 856
TseI GCWGC 3 cut(s) 240, 409, 821
TspDTI ATGAA 2 cut(s) 100, 888
TspGWI ACGGA 1 cut(s) 351
TspMI CCCGGG 1 cut(s) 776
TspRI CASTG 3 cut(s) 542, 706, 856
Tth111I GACNNNGTC 1 cut(s) 518
XbaI TCTAGA 1 cut(s) 908
XceI RCATGY 1 cut(s) 42
XhoI CTCGAG 1 cut(s) 988
XmaI CCCGGG 1 cut(s) 776
XmaJI CCTAGG 1 cut(s) 887
XspI CTAG 2 cut(s) 888, 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.