MD04G1131800.v1.1

E3 ubiquitin-protein ligase RING1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001214
Physical Location & Seq
Reverse (-)
21863842 .. 21864909
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1131800.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGCTTCCATGGCAGCATCGTCCTACTGGTGCTACACGTGTAGCCGATCGATTCAAGTTCGGGTTCGGACCCAAGATTCGATCCTCTGCCCCGATTGCGGAGCTGGGTTTATTGAAGAGATCCCGACCCCTACCCGATCTTCTCCGCTACACCACCCATTTCCCACCGCCGCGATGTTCCTAGACAGCGCCCCGGTTTCTCAGAGTCCGAGCCCGCCTAGACTTCGCCGGAATCAGAGAAGCGGCCGCGACCGGTCCCCGTTTAACCCGGTCATCGTTCTCCGGGGCCCACCTGATACTGAATCTGACCCGGGAAGCTTCGAGCTGTTCTACGATGACGGAGCCGGGTTAGGCCTCCGCCCTCTTCCGTCGTCCATGTCGGATATCTTAATGGGGTCGGGTTTCGACCGGTTGCTCCACCAGTTGACCCAATTGGAAAATGGCGTCGCGCGGCTGGACCACCCTCCGGCATCCAAAGCCGCAATCGAGTCCATGCCCGTCATTAAAATCGCCGACAGCCACGTCGAGACGGAGTCGCACTGTGCCGTTTGCAAAGAGGCATTCGAGCTCGACTCGGAAGCTCGCGAGATGCCGTGTAAGCACATCTACCACTCAGATTGTATCTTCCCGTGGCTTTCAATCCGCAATTCGTGCCCGGTTTGCCGGCACCAGCTCCCGACTGATGTGAGAGGCCCGCGCGGCAGTGGCAGAGCATCGCCGGAGAACAATGGCGTGGTTGGAGAAGAAGAGCCAGTGGGGTTGACTATTTGGAGATTGCCCGGTGGTGGATTTGCCGTGGGGAGGTTCACTGGAGGCAGAAGAGCCGCAGAGCGTGAGCTCCCAGTTGTTTACACCGAGATGGATGGTGGGTTCAATACGGCGGGTGCTCCTAGGAGGATTTCATGGGCATCTAGAAGAAGGGCCAGAGAGAGTGGTGGATTTGCTCAGGCTTTTCGCAATTTCTTCTCTTTTTTCGGGAGGCTGAGAAGCTCGAGAAGTCGGTCTGGTAATGTGTTCAGTAGATCATCGCGGCGGAGGAGCCAGCGTTGGGCTTTGGAAGACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1117 zf-RING_2 zinc_ribbon_9

Protein Analysis

356

Amino Acids

39.24

Weight (kDa)

9.02

Isoelectric Point (pI)

81.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 7 - 41 1.3e-13 zinc-ribbon
zf-rbx1 PF12678 176 - 222 1.3e-06 RING-H2 zinc finger domain
zf-RING_2 PF13639 180 - 222 5.4e-12 Ring finger domain
zf-C3HC4_2 PF13923 181 - 221 3.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 181 - 221 9.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 181 - 208 2.9e-06 RING-like zinc finger
DUF1117 PF06547 252 - 348 2.2e-38 Protein of unknown function (DUF1117)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 521
AccB1I GGYRCC 1 cut(s) 666
AccII CGCG 8 cut(s) 173, 249, 449, 451, 585, 697, 699, 1030
AclWI GGATC 2 cut(s) 76, 115
AcoI YGGCCR 1 cut(s) 244
AcvI CACGTG 1 cut(s) 39
AcyI GRCGYC 1 cut(s) 444
AfiI CCNNNNNNNGG 5 cut(s) 98, 466, 785, 801, 1048
AflIII ACRYGT 2 cut(s) 36, 38
AgeI ACCGGT 2 cut(s) 252, 408
AgsI TTSAA 4 cut(s) 56, 116, 639, 874
AjiI CACGTC 1 cut(s) 523
AloI GAACNNNNNNTCC 2 cut(s) 854, 886
AluBI AGCT 8 cut(s) 104, 318, 325, 568, 581, 673, 838, 990
AluI AGCT 8 cut(s) 104, 318, 325, 568, 581, 673, 838, 990
Alw21I GWGCWC 3 cut(s) 570, 840, 889
Alw26I GTCTC 1 cut(s) 521
AlwI GGATC 2 cut(s) 76, 115
Ama87I CYCGRG 2 cut(s) 310, 991
AoxI GGCC 5 cut(s) 244, 286, 352, 691, 921
ApaI GGGCCC 1 cut(s) 290
ApeKI GCWGC 1 cut(s) 14
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
AsiGI ACCGGT 2 cut(s) 252, 408
AspA2I CCTAGG 1 cut(s) 890
AspLEI GCGC 3 cut(s) 191, 451, 699
AspS9I GGNCC 7 cut(s) 69, 255, 286, 287, 457, 692, 921
AsuC2I CCSGG 8 cut(s) 194, 269, 284, 311, 312, 346, 656, 780
AvaI CYCGRG 2 cut(s) 310, 991
AvaII GGWCC 3 cut(s) 69, 255, 457
AvrII CCTAGG 1 cut(s) 890
BaeGI GKGCMC 2 cut(s) 290, 656
BanI GGYRCC 1 cut(s) 666
BanII GRGCYC 4 cut(s) 215, 290, 570, 840
BbrPI CACGTG 1 cut(s) 39
Bbv12I GWGCWC 3 cut(s) 570, 840, 889
BbvI GCAGC 1 cut(s) 26
BccI CCATC 2 cut(s) 853, 857
BceAI ACGGC 4 cut(s) 530, 577, 779, 894
BcnI CCSGG 8 cut(s) 194, 269, 284, 311, 312, 346, 656, 780
BcoDI GTCTC 1 cut(s) 521
BfaI CTAG 4 cut(s) 182, 219, 891, 912
BfoI RGCGCY 1 cut(s) 192
BglI GCCNNNNNGGC 1 cut(s) 699
BisI GCNGC 9 cut(s) 15, 171, 244, 247, 452, 480, 700, 825, 1031
BlnI CCTAGG 1 cut(s) 890
BlsI GCNGC 9 cut(s) 16, 172, 245, 248, 453, 481, 701, 826, 1032
Bme1390I CCNGG 8 cut(s) 194, 269, 284, 311, 312, 346, 656, 780
Bme18I GGWCC 3 cut(s) 69, 255, 457
BmeT110I CYCGRG 2 cut(s) 310, 991
BmgBI CACGTC 1 cut(s) 523
BmgT120I GGNCC 7 cut(s) 69, 255, 286, 287, 457, 692, 921
BmiI GGNNCC 7 cut(s) 71, 257, 287, 288, 343, 668, 1040
BmrFI CCNGG 8 cut(s) 194, 269, 284, 311, 312, 346, 656, 780
BmrI ACTGGG 1 cut(s) 836
BmsI GCATC 5 cut(s) 26, 479, 579, 722, 917
BmuI ACTGGG 1 cut(s) 836
BplI GAGNNNNNCTC 2 cut(s) 557, 589
BpmI CTGGAG 1 cut(s) 831
Bpu10I CCTNAGC 1 cut(s) 945
BpuMI CCSGG 8 cut(s) 194, 269, 284, 311, 312, 346, 656, 780
Bsa29I ATCGAT 1 cut(s) 50
BsaAI YACGTR 1 cut(s) 39
BsaHI GRCGYC 1 cut(s) 444
BsaJI CCNNGG 7 cut(s) 9, 192, 283, 310, 629, 795, 890
BsaWI WCCGGW 2 cut(s) 252, 408
BsaXI ACNNNNNCTCC 2 cut(s) 1030, 1060
Bsc4I CCNNNNNNNGG 5 cut(s) 98, 466, 785, 801, 1048
Bse118I RCCGGY 3 cut(s) 252, 408, 663
Bse1I ACTGG 5 cut(s) 32, 421, 752, 814, 842
BseCI ATCGAT 1 cut(s) 50
BseDI CCNNGG 7 cut(s) 9, 192, 283, 310, 629, 795, 890
BseGI GGATG 2 cut(s) 470, 868
BseLI CCNNNNNNNGG 5 cut(s) 98, 466, 785, 801, 1048
BseMII CTCAG 4 cut(s) 215, 627, 959, 974
BseNI ACTGG 5 cut(s) 32, 421, 752, 814, 842
BseRI GAGGAG 1 cut(s) 1051
BseSI GKGCMC 2 cut(s) 290, 656
BseX3I CGGCCG 1 cut(s) 244
BseXI GCAGC 1 cut(s) 26
BseYI CCCAGC 1 cut(s) 104
Bsh1236I CGCG 8 cut(s) 173, 249, 449, 451, 585, 697, 699, 1030
Bsh1285I CGRYCG 4 cut(s) 50, 247, 253, 409
BshFI GGCC 5 cut(s) 246, 288, 354, 693, 923
BshNI GGYRCC 1 cut(s) 666
BshTI ACCGGT 2 cut(s) 252, 408
BshVI ATCGAT 1 cut(s) 50
BsiEI CGRYCG 4 cut(s) 50, 247, 253, 409
BsiHKAI GWGCWC 3 cut(s) 570, 840, 889
BsiHKCI CYCGRG 2 cut(s) 310, 991
BslFI GGGAC 1 cut(s) 241
BslI CCNNNNNNNGG 5 cut(s) 98, 466, 785, 801, 1048
BsmAI GTCTC 1 cut(s) 521
BsmBI CGTCTC 1 cut(s) 521
BsmFI GGGAC 1 cut(s) 241
BsmI GAATGC 1 cut(s) 560
BsnI GGCC 5 cut(s) 246, 288, 354, 693, 923
BsoBI CYCGRG 2 cut(s) 310, 991
Bsp120I GGGCCC 1 cut(s) 286
Bsp1286I GDGCHC 6 cut(s) 215, 290, 570, 656, 840, 889
Bsp143I GATC 5 cut(s) 47, 81, 120, 137, 1022
Bsp19I CCATGG 1 cut(s) 9
Bsp68I TCGCGA 1 cut(s) 585
BspANI GGCC 5 cut(s) 246, 288, 354, 693, 923
BspCNI CTCAG 4 cut(s) 214, 626, 958, 975
BspDI ATCGAT 1 cut(s) 50
BspFNI CGCG 8 cut(s) 173, 249, 449, 451, 585, 697, 699, 1030
BspLI GGNNCC 7 cut(s) 71, 257, 287, 288, 343, 668, 1040
BspPI GGATC 2 cut(s) 76, 115
BspQI GCTCTTC 2 cut(s) 741, 814
BspT107I GGYRCC 1 cut(s) 666
BsrFI RCCGGY 3 cut(s) 252, 408, 663
BsrI ACTGG 5 cut(s) 32, 421, 752, 814, 842
BssAI RCCGGY 3 cut(s) 252, 408, 663
BssECI CCNNGG 7 cut(s) 9, 192, 283, 310, 629, 795, 890
BssMI GATC 5 cut(s) 47, 81, 120, 137, 1022
BssNI GRCGYC 1 cut(s) 444
BssT1I CCWWGG 2 cut(s) 9, 890
Bst4CI ACNGT 1 cut(s) 542
Bst6I CTCTTC 4 cut(s) 111, 369, 741, 814
BstACI GRCGYC 1 cut(s) 444
BstAPI GCANNNNNTGC 1 cut(s) 651
BstBAI YACGTR 1 cut(s) 39
BstC8I GCNNGC 5 cut(s) 215, 583, 665, 695, 1043
BstDEI CTNAG 4 cut(s) 201, 613, 945, 983
BstDSI CCRYGG 3 cut(s) 9, 629, 795
BstF5I GGATG 2 cut(s) 470, 868
BstFNI CGCG 8 cut(s) 173, 249, 449, 451, 585, 697, 699, 1030
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 3 cut(s) 191, 451, 699
BstKTI GATC 5 cut(s) 50, 84, 123, 140, 1025
BstMAI GTCTC 1 cut(s) 521
BstMBI GATC 5 cut(s) 47, 81, 120, 137, 1022
BstMCI CGRYCG 4 cut(s) 50, 247, 253, 409
BstMWI GCNNNNNNNGC 8 cut(s) 11, 96, 476, 598, 651, 660, 699, 705
BstSCI CCNGG 8 cut(s) 192, 267, 282, 309, 310, 344, 654, 778
BstSLI GKGCMC 2 cut(s) 290, 656
BstUI CGCG 8 cut(s) 173, 249, 449, 451, 585, 697, 699, 1030
BstV1I GCAGC 1 cut(s) 26
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
BstZI CGGCCG 1 cut(s) 244
Bsu15I ATCGAT 1 cut(s) 50
BsuRI GGCC 5 cut(s) 246, 288, 354, 693, 923
BsuTUI ATCGAT 1 cut(s) 50
BtgI CCRYGG 3 cut(s) 9, 629, 795
BtgZI GCGATG 3 cut(s) 188, 699, 1011
BtrI CACGTC 1 cut(s) 523
BtsCI GGATG 2 cut(s) 470, 868
BtsI GCAGTG 1 cut(s) 709
BtsIMutI CAGTG 4 cut(s) 538, 709, 759, 807
BtuMI TCGCGA 1 cut(s) 585
Cac8I GCNNGC 5 cut(s) 215, 583, 665, 695, 1043
CciNI GCGGCCGC 1 cut(s) 244
CfoI GCGC 3 cut(s) 191, 451, 699
Cfr10I RCCGGY 3 cut(s) 252, 408, 663
Cfr13I GGNCC 7 cut(s) 69, 255, 286, 287, 457, 692, 921
Cfr9I CCCGGG 1 cut(s) 310
ClaI ATCGAT 1 cut(s) 50
CseI GACGC 1 cut(s) 433
CspAI ACCGGT 2 cut(s) 252, 408
CspCI CAANNNNNGTGG 2 cut(s) 599, 634
CviAII CATG 4 cut(s) 10, 376, 493, 903
DdeI CTNAG 4 cut(s) 201, 613, 945, 983
DpnI GATC 5 cut(s) 49, 83, 122, 139, 1024
DpnII GATC 5 cut(s) 47, 81, 120, 137, 1022
DrdI GACNNNNNNGTC 1 cut(s) 521
DseDI GACNNNNNNGTC 1 cut(s) 521
EaeI YGGCCR 1 cut(s) 244
EagI CGGCCG 1 cut(s) 244
Eam1104I CTCTTC 4 cut(s) 111, 369, 741, 814
EarI CTCTTC 4 cut(s) 111, 369, 741, 814
EciI GGCGGA 2 cut(s) 347, 1048
Ecl136II GAGCTC 2 cut(s) 568, 838
EclXI CGGCCG 1 cut(s) 244
Eco130I CCWWGG 2 cut(s) 9, 890
Eco147I AGGCCT 1 cut(s) 354
Eco24I GRGCYC 4 cut(s) 215, 290, 570, 840
Eco32I GATATC 1 cut(s) 385
Eco47I GGWCC 3 cut(s) 69, 255, 457
Eco52I CGGCCG 1 cut(s) 244
Eco53kI GAGCTC 2 cut(s) 568, 838
Eco72I CACGTG 1 cut(s) 39
Eco88I CYCGRG 2 cut(s) 310, 991
EcoICRI GAGCTC 2 cut(s) 568, 838
EcoO109I RGGNCCY 1 cut(s) 286
EcoRV GATATC 1 cut(s) 385
EcoT14I CCWWGG 2 cut(s) 9, 890
EcoT38I GRGCYC 4 cut(s) 215, 290, 570, 840
ErhI CCWWGG 2 cut(s) 9, 890
Esp3I CGTCTC 1 cut(s) 521
FaeI CATG 4 cut(s) 13, 379, 496, 906
FaiI YATR 4 cut(s) 11, 377, 494, 904
FaqI GGGAC 1 cut(s) 241
FatI CATG 4 cut(s) 9, 375, 492, 902
FauI CCCGC 3 cut(s) 222, 702, 874
Fnu4HI GCNGC 9 cut(s) 15, 171, 244, 247, 452, 480, 700, 825, 1031
FokI GGATG 2 cut(s) 457, 875
FriOI GRGCYC 4 cut(s) 215, 290, 570, 840
Fsp4HI GCNGC 9 cut(s) 15, 171, 244, 247, 452, 480, 700, 825, 1031
FspBI CTAG 4 cut(s) 182, 219, 891, 912
GlaI GCGC 3 cut(s) 190, 450, 698
GluI GCNGC 9 cut(s) 15, 171, 244, 247, 452, 480, 700, 825, 1031
GsaI CCCAGC 1 cut(s) 108
GsuI CTGGAG 1 cut(s) 831
HaeII RGCGCY 1 cut(s) 192
HaeIII GGCC 5 cut(s) 246, 288, 354, 693, 923
HgaI GACGC 1 cut(s) 433
HhaI GCGC 3 cut(s) 191, 451, 699
Hin1I GRCGYC 1 cut(s) 444
Hin1II CATG 4 cut(s) 13, 379, 496, 906
Hin6I GCGC 3 cut(s) 189, 449, 697
HinP1I GCGC 3 cut(s) 189, 449, 697
HincII GTYRAC 2 cut(s) 426, 762
HindII GTYRAC 2 cut(s) 426, 762
HindIII AAGCTT 1 cut(s) 316
HinfI GANTC 8 cut(s) 52, 77, 205, 232, 302, 488, 533, 572
Hpy166II GTNNAC 4 cut(s) 426, 762, 807, 850
Hpy188I TCNGA 8 cut(s) 69, 204, 210, 237, 307, 382, 577, 616
Hpy188III TCNNGA 7 cut(s) 124, 526, 584, 676, 912, 976, 993
Hpy8I GTNNAC 4 cut(s) 426, 762, 807, 850
Hpy99I CGWCG 3 cut(s) 373, 449, 527
HpyAV CCTTC 1 cut(s) 912
HpyCH4III ACNGT 1 cut(s) 542
HpyCH4IV ACGT 2 cut(s) 38, 522
HpyCH4V TGCA 1 cut(s) 552
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 96, 476, 598, 651, 660, 699, 705
HpyF3I CTNAG 4 cut(s) 201, 613, 945, 983
HpySE526I ACGT 2 cut(s) 38, 522
Hsp92I GRCGYC 1 cut(s) 444
Hsp92II CATG 4 cut(s) 13, 379, 496, 906
HspAI GCGC 3 cut(s) 189, 449, 697
KroI GCCGGC 1 cut(s) 663
KroNI GCCGGC 1 cut(s) 665
Kzo9I GATC 5 cut(s) 47, 81, 120, 137, 1022
LguI GCTCTTC 2 cut(s) 741, 814
LmnI GCTCC 7 cut(s) 101, 341, 420, 678, 843, 892, 1038
Lsp1109I GCAGC 1 cut(s) 26
LweI GCATC 5 cut(s) 26, 479, 579, 722, 917
MaeI CTAG 4 cut(s) 182, 219, 891, 912
MaeII ACGT 2 cut(s) 38, 522
MalI GATC 5 cut(s) 49, 83, 122, 139, 1024
MboI GATC 5 cut(s) 47, 81, 120, 137, 1022
MboII GAAGA 9 cut(s) 128, 132, 356, 616, 755, 758, 831, 927, 955
MfeI CAATTG 1 cut(s) 431
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 6 cut(s) 215, 290, 570, 656, 840, 889
MluCI AATT 3 cut(s) 431, 646, 958
MlyI GAGTC 4 cut(s) 214, 497, 542, 566
MmeI TCCRAC 2 cut(s) 360, 718
MroNI GCCGGC 1 cut(s) 663
MseI TTAA 3 cut(s) 264, 389, 504
MslI CAYNNNNRTG 1 cut(s) 857
MspR9I CCNGG 8 cut(s) 194, 269, 284, 311, 312, 346, 656, 780
MunI CAATTG 1 cut(s) 431
Mva1269I GAATGC 1 cut(s) 560
MvnI CGCG 8 cut(s) 173, 249, 449, 451, 585, 697, 699, 1030
MwoI GCNNNNNNNGC 8 cut(s) 11, 96, 476, 598, 651, 660, 699, 705
NaeI GCCGGC 1 cut(s) 665
NciI CCSGG 8 cut(s) 194, 269, 284, 311, 312, 346, 656, 780
NcoI CCATGG 1 cut(s) 9
NdeII GATC 5 cut(s) 47, 81, 120, 137, 1022
NgoMIV GCCGGC 1 cut(s) 663
NlaIII CATG 4 cut(s) 13, 379, 496, 906
NlaIV GGNNCC 7 cut(s) 71, 257, 287, 288, 343, 668, 1040
NotI GCGGCCGC 1 cut(s) 244
NruI TCGCGA 1 cut(s) 585
PaeR7I CTCGAG 1 cut(s) 991
PceI AGGCCT 1 cut(s) 354
PciSI GCTCTTC 2 cut(s) 741, 814
PctI GAATGC 1 cut(s) 560
PdiI GCCGGC 1 cut(s) 665
PfeI GAWTC 4 cut(s) 52, 77, 232, 302
PflFI GACNNNGTC 1 cut(s) 532
PinAI ACCGGT 2 cut(s) 252, 408
PkrI GCNGC 9 cut(s) 16, 172, 245, 248, 453, 481, 701, 826, 1032
Ple19I CGATCG 1 cut(s) 50
PleI GAGTC 4 cut(s) 213, 496, 541, 566
PmaCI CACGTG 1 cut(s) 39
PmlI CACGTG 1 cut(s) 39
PpsI GAGTC 4 cut(s) 213, 496, 541, 566
Ppu21I YACGTR 1 cut(s) 39
Psp124BI GAGCTC 2 cut(s) 570, 840
PspCI CACGTG 1 cut(s) 39
PspFI CCCAGC 1 cut(s) 104
PspN4I GGNNCC 7 cut(s) 71, 257, 287, 288, 343, 668, 1040
PspOMI GGGCCC 1 cut(s) 286
PspPI GGNCC 7 cut(s) 69, 255, 286, 287, 457, 692, 921
PsuI RGATCY 1 cut(s) 120
PsyI GACNNNGTC 1 cut(s) 532
PvuI CGATCG 1 cut(s) 50
RruI TCGCGA 1 cut(s) 585
RseI CAYNNNNRTG 1 cut(s) 857
SacI GAGCTC 2 cut(s) 570, 840
SapI GCTCTTC 2 cut(s) 741, 814
SaqAI TTAA 3 cut(s) 264, 389, 504
SatI GCNGC 9 cut(s) 15, 171, 244, 247, 452, 480, 700, 825, 1031
Sau3AI GATC 5 cut(s) 47, 81, 120, 137, 1022
Sau96I GGNCC 7 cut(s) 69, 255, 286, 287, 457, 692, 921
SchI GAGTC 4 cut(s) 214, 497, 542, 566
ScrFI CCNGG 8 cut(s) 194, 269, 284, 311, 312, 346, 656, 780
SduI GDGCHC 6 cut(s) 215, 290, 570, 656, 840, 889
SfaNI GCATC 5 cut(s) 26, 479, 579, 722, 917
Sfr274I CTCGAG 1 cut(s) 991
SinI GGWCC 3 cut(s) 69, 255, 457
SlaI CTCGAG 1 cut(s) 991
SmaI CCCGGG 1 cut(s) 312
SmiMI CAYNNNNRTG 1 cut(s) 857
SmlI CTYRAG 1 cut(s) 991
SmoI CTYRAG 1 cut(s) 991
Sse9I AATT 3 cut(s) 431, 646, 958
SseBI AGGCCT 1 cut(s) 354
SspMI CTAG 4 cut(s) 182, 219, 891, 912
SstI GAGCTC 2 cut(s) 570, 840
StuI AGGCCT 1 cut(s) 354
StyD4I CCNGG 8 cut(s) 192, 267, 282, 309, 310, 344, 654, 778
StyI CCWWGG 2 cut(s) 9, 890
TaaI ACNGT 1 cut(s) 542
TaiI ACGT 2 cut(s) 41, 525
TaqI TCGA 9 cut(s) 50, 80, 321, 405, 486, 525, 564, 570, 992
TaqII GACCGA 1 cut(s) 990
TasI AATT 3 cut(s) 431, 646, 958
TauI GCSGC 8 cut(s) 173, 246, 249, 454, 482, 702, 827, 1033
TfiI GAWTC 4 cut(s) 52, 77, 232, 302
Tru1I TTAA 3 cut(s) 264, 389, 504
Tru9I TTAA 3 cut(s) 264, 389, 504
TscAI CASTG 4 cut(s) 545, 709, 759, 814
TseI GCWGC 1 cut(s) 14
TspDTI ATGAA 1 cut(s) 891
TspGWI ACGGA 3 cut(s) 354, 357, 545
TspMI CCCGGG 1 cut(s) 310
TspRI CASTG 4 cut(s) 545, 709, 759, 814
Tth111I GACNNNGTC 1 cut(s) 532
VpaK11BI GGWCC 3 cut(s) 69, 255, 457
XbaI TCTAGA 1 cut(s) 911
XcmI CCANNNNNNNNNTGG 1 cut(s) 437
XhoI CTCGAG 1 cut(s) 991
XmaI CCCGGG 1 cut(s) 310
XmaJI CCTAGG 1 cut(s) 890
XspI CTAG 4 cut(s) 182, 219, 891, 912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.