Rorug03G0080500

E3 ubiquitin-protein ligase RING1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0001214
Physical Location & Seq
Forward (+)
6295458 .. 6297162
1705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0080500.1

Sequence Viewer

Length: 957 bp
ATGGGTGTGAAGCAAGGTTCCGAGCCAACGATCGTGATCGACCGTCTAAAGTTCACGTACCCGGGAATCAACGGCCACCCGCCGCCCGGCGCCAAGCCTCTGATCGACGACTTCTCCCTCACGCTCAACGCCGGCGACCGATGCCTCCTAGTCGGATCCAACGGCGCTGGGAAAACCACGATCCTCAAGATTCTCGGAGGCAAGCACATGGTCGACCCCAGCATGGTCCGGGTTCTGGGTCGGTCGGCTTTCCACGACACCGCTCTCACCGGCTCCGGCGACCTGTCGTATCTCGGCGGAGAGTGGCGGCGTGACGTGGCGTTCGCCGGGTTCGACGTTCCGATACAGATGGATGTTTCGGCTGAGAAATTGATTAACGGGGTGGCTGGGATTGACCCGCAAAGGAGAGCTGAGCTGATCAAGGTGTTGGATATTGATCTTTCATGGAGGTTGCATAAGGTCTCAGACGGTCAGAGAAGAAGGGTCCAGATCTGTATGGGTCTGCTCAGACCATACAAGGTGCTTCTGCTTGATGAGATCACAGTGGACCTTGATGTGCTAGCGAGGGCGGACCTTCTCCAGTTCTTGAGGAAGGAATGTGAGGAGCGAGGTGCTACGATCATTTACGCTACGCATATATTTGACGGGTTGGAGAATTGGCCATCACACATTGTGTATGTTGCTAATGGGAAGCTGCAATTGTCAATGCCGATGGACAAGGTTAGGGAGACGTACAAGCTGTCGCTCATGAGAACAGTTGAGAGTTGGCTGAGGAAGGAGCGCGAGGAGGAGAGGGCGAGAAGAAAAGAGAGGAAGGCAAGAGGTCTTGCCGAGTTTGATGAACAAATTGAGGGCAGCCGTGTGATTAATAATGGTTGGGCGGCCGGGCGCCTCAACTCCACCGTCGCCGGAGAAGAAAACTTTGTGTGGAGCTCAAACAGAGTTCTCAGGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1117 zf-RING_2 zinc_ribbon_9

Protein Analysis

318

Amino Acids

35.66

Weight (kDa)

8.9

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 36 - 181 2.1e-16 ABC transporter
AAA_21 PF13304 149 - 212 1.1e-08 AAA domain, putative AbiEii toxin, Type IV TA system
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 89, 888
AccBSI CCGCTC 1 cut(s) 263
AccI GTMKAC 1 cut(s) 213
AccII CGCG 1 cut(s) 783
AciI CCGC 8 cut(s) 80, 83, 261, 297, 307, 398, 569, 881
AclWI GGATC 3 cut(s) 150, 163, 175
AcoI YGGCCR 3 cut(s) 73, 659, 882
AcyI GRCGYC 2 cut(s) 90, 889
AdeI CACNNNGTG 1 cut(s) 673
AfaI GTAC 2 cut(s) 59, 734
AfiI CCNNNNNNNGG 3 cut(s) 86, 223, 235
AjiI CACGTC 1 cut(s) 316
AluBI AGCT 5 cut(s) 410, 415, 694, 739, 933
AluI AGCT 5 cut(s) 410, 415, 694, 739, 933
Alw21I GWGCWC 1 cut(s) 935
Alw26I GTCTC 2 cut(s) 466, 722
AlwI GGATC 3 cut(s) 150, 163, 175
Ama87I CYCGRG 1 cut(s) 61
AoxI GGCC 3 cut(s) 73, 659, 882
ApeKI GCWGC 2 cut(s) 694, 855
ArsI GACNNNNNNTTYG 2 cut(s) 305, 337
AseI ATTAAT 1 cut(s) 867
AspLEI GCGC 4 cut(s) 92, 167, 783, 891
AspS9I GGNCC 4 cut(s) 226, 484, 547, 571
AsuC2I CCSGG 6 cut(s) 62, 63, 87, 230, 328, 886
AsuHPI GGTGA 1 cut(s) 259
AsuNHI GCTAGC 1 cut(s) 559
AvaI CYCGRG 1 cut(s) 61
AvaII GGWCC 4 cut(s) 226, 484, 547, 571
BalI TGGCCA 1 cut(s) 661
BamHI GGATCC 1 cut(s) 155
BanI GGYRCC 2 cut(s) 89, 888
BanII GRGCYC 1 cut(s) 935
Bbv12I GWGCWC 1 cut(s) 935
BbvCI CCTCAGC 1 cut(s) 770
BbvI GCAGC 2 cut(s) 681, 867
BccI CCATC 3 cut(s) 343, 670, 706
BceAI ACGGC 3 cut(s) 88, 178, 843
BclI TGATCA 1 cut(s) 417
BcnI CCSGG 6 cut(s) 62, 63, 87, 230, 328, 886
BcoDI GTCTC 2 cut(s) 466, 722
BfaI CTAG 2 cut(s) 149, 560
BfoI RGCGCY 3 cut(s) 93, 168, 892
BglII AGATCT 1 cut(s) 489
BisI GCNGC 5 cut(s) 83, 308, 695, 856, 882
BlpI GCTNAGC 1 cut(s) 411
BlsI GCNGC 5 cut(s) 84, 309, 696, 857, 883
Bme1390I CCNGG 6 cut(s) 62, 63, 87, 230, 328, 886
Bme18I GGWCC 4 cut(s) 226, 484, 547, 571
BmeT110I CYCGRG 1 cut(s) 61
BmgBI CACGTC 1 cut(s) 316
BmgT120I GGNCC 4 cut(s) 226, 484, 547, 571
BmiI GGNNCC 6 cut(s) 19, 91, 157, 274, 485, 890
BmrFI CCNGG 6 cut(s) 62, 63, 87, 230, 328, 886
BmsI GCATC 1 cut(s) 131
BmtI GCTAGC 1 cut(s) 563
BpmI CTGGAG 1 cut(s) 563
Bpu10I CCTNAGC 1 cut(s) 770
Bpu1102I GCTNAGC 1 cut(s) 411
BpuEI CTTGAG 2 cut(s) 170, 607
BpuMI CCSGG 6 cut(s) 62, 63, 87, 230, 328, 886
BsaAI YACGTR 1 cut(s) 57
BsaBI GATNNNNATC 2 cut(s) 35, 435
BsaHI GRCGYC 2 cut(s) 90, 889
BsaI GGTCTC 1 cut(s) 466
BsaJI CCNNGG 1 cut(s) 61
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 3 cut(s) 86, 223, 235
Bse118I RCCGGY 2 cut(s) 131, 269
Bse1I ACTGG 1 cut(s) 580
Bse8I GATNNNNATC 2 cut(s) 35, 435
BseDI CCNNGG 1 cut(s) 61
BseGI GGATG 1 cut(s) 358
BseJI GATNNNNATC 2 cut(s) 35, 435
BseLI CCNNNNNNNGG 3 cut(s) 86, 223, 235
BseMII CTCAG 5 cut(s) 354, 402, 477, 520, 761
BseNI ACTGG 1 cut(s) 580
BseRI GAGGAG 3 cut(s) 617, 800, 803
BseX3I CGGCCG 1 cut(s) 882
BseXI GCAGC 2 cut(s) 681, 867
BseYI CCCAGC 3 cut(s) 167, 218, 386
Bsh1236I CGCG 1 cut(s) 783
Bsh1285I CGRYCG 5 cut(s) 33, 43, 139, 245, 885
BshFI GGCC 3 cut(s) 75, 661, 884
BshNI GGYRCC 2 cut(s) 89, 888
BsiEI CGRYCG 5 cut(s) 33, 43, 139, 245, 885
BsiHKAI GWGCWC 1 cut(s) 935
BsiHKCI CYCGRG 1 cut(s) 61
BsiSI CCGG 9 cut(s) 62, 87, 132, 229, 270, 276, 327, 885, 909
BslI CCNNNNNNNGG 3 cut(s) 86, 223, 235
BsmAI GTCTC 2 cut(s) 466, 722
BsmBI CGTCTC 1 cut(s) 722
BsnI GGCC 3 cut(s) 75, 661, 884
Bso31I GGTCTC 1 cut(s) 466
BsoBI CYCGRG 1 cut(s) 61
Bsp1286I GDGCHC 1 cut(s) 935
Bsp1720I GCTNAGC 1 cut(s) 411
BspACI CCGC 8 cut(s) 80, 83, 261, 297, 307, 398, 569, 881
BspANI GGCC 3 cut(s) 75, 661, 884
BspCNI CTCAG 5 cut(s) 355, 403, 476, 519, 762
BspFNI CGCG 1 cut(s) 783
BspHI TCATGA 1 cut(s) 747
BspLI GGNNCC 6 cut(s) 19, 91, 157, 274, 485, 890
BspOI GCTAGC 1 cut(s) 563
BspPI GGATC 3 cut(s) 150, 163, 175
BspT107I GGYRCC 2 cut(s) 89, 888
BspTNI GGTCTC 1 cut(s) 466
BsrBI CCGCTC 1 cut(s) 263
BsrFI RCCGGY 2 cut(s) 131, 269
BsrI ACTGG 1 cut(s) 580
BssAI RCCGGY 2 cut(s) 131, 269
BssECI CCNNGG 1 cut(s) 61
BssNI GRCGYC 2 cut(s) 90, 889
Bst4CI ACNGT 5 cut(s) 44, 470, 544, 757, 904
BstACI GRCGYC 2 cut(s) 90, 889
BstBAI YACGTR 1 cut(s) 57
BstC8I GCNNGC 3 cut(s) 133, 203, 561
BstDEI CTNAG 6 cut(s) 363, 411, 463, 506, 770, 947
BstF5I GGATG 1 cut(s) 358
BstFNI CGCG 1 cut(s) 783
BstH2I RGCGCY 3 cut(s) 93, 168, 892
BstHHI GCGC 4 cut(s) 92, 167, 783, 891
BstMAI GTCTC 2 cut(s) 466, 722
BstMCI CGRYCG 5 cut(s) 33, 43, 139, 245, 885
BstMWI GCNNNNNNNGC 1 cut(s) 141
BstSCI CCNGG 6 cut(s) 60, 61, 85, 228, 326, 884
BstUI CGCG 1 cut(s) 783
BstV1I GCAGC 2 cut(s) 681, 867
BstX2I RGATCY 2 cut(s) 155, 489
BstYI RGATCY 2 cut(s) 155, 489
BstZI CGGCCG 1 cut(s) 882
BsuRI GGCC 3 cut(s) 75, 661, 884
BtrI CACGTC 1 cut(s) 316
BtsCI GGATG 1 cut(s) 358
BtsIMutI CAGTG 1 cut(s) 549
Cac8I GCNNGC 3 cut(s) 133, 203, 561
CciI TCATGA 1 cut(s) 747
CfoI GCGC 4 cut(s) 92, 167, 783, 891
Cfr10I RCCGGY 2 cut(s) 131, 269
Cfr13I GGNCC 4 cut(s) 226, 484, 547, 571
Cfr9I CCCGGG 1 cut(s) 61
Csp6I GTAC 2 cut(s) 58, 733
CviAII CATG 4 cut(s) 208, 223, 444, 748
CviQI GTAC 2 cut(s) 58, 733
DdeI CTNAG 6 cut(s) 363, 411, 463, 506, 770, 947
DinI GGCGCC 2 cut(s) 91, 890
DraIII CACNNNGTG 1 cut(s) 673
EaeI YGGCCR 3 cut(s) 73, 659, 882
EagI CGGCCG 1 cut(s) 882
EciI GGCGGA 2 cut(s) 312, 584
Ecl136II GAGCTC 1 cut(s) 933
EclXI CGGCCG 1 cut(s) 882
Eco24I GRGCYC 1 cut(s) 935
Eco31I GGTCTC 1 cut(s) 466
Eco47I GGWCC 4 cut(s) 226, 484, 547, 571
Eco52I CGGCCG 1 cut(s) 882
Eco53kI GAGCTC 1 cut(s) 933
Eco88I CYCGRG 1 cut(s) 61
EcoICRI GAGCTC 1 cut(s) 933
EcoT38I GRGCYC 1 cut(s) 935
EgeI GGCGCC 2 cut(s) 91, 890
EheI GGCGCC 2 cut(s) 91, 890
Esp3I CGTCTC 1 cut(s) 722
FaeI CATG 4 cut(s) 211, 226, 447, 751
FatI CATG 4 cut(s) 207, 222, 443, 747
FauI CCCGC 2 cut(s) 87, 405
FbaI TGATCA 1 cut(s) 417
FblI GTMKAC 1 cut(s) 213
Fnu4HI GCNGC 5 cut(s) 83, 308, 695, 856, 882
FokI GGATG 1 cut(s) 365
FriOI GRGCYC 1 cut(s) 935
Fsp4HI GCNGC 5 cut(s) 83, 308, 695, 856, 882
FspBI CTAG 2 cut(s) 149, 560
GlaI GCGC 4 cut(s) 91, 166, 782, 890
GluI GCNGC 5 cut(s) 83, 308, 695, 856, 882
GsaI CCCAGC 3 cut(s) 171, 222, 390
GsuI CTGGAG 1 cut(s) 563
HaeII RGCGCY 3 cut(s) 93, 168, 892
HaeIII GGCC 3 cut(s) 75, 661, 884
HapII CCGG 9 cut(s) 62, 87, 132, 229, 270, 276, 327, 885, 909
HhaI GCGC 4 cut(s) 92, 167, 783, 891
Hin1I GRCGYC 2 cut(s) 90, 889
Hin1II CATG 4 cut(s) 211, 226, 447, 751
Hin6I GCGC 4 cut(s) 90, 165, 781, 889
HinP1I GCGC 4 cut(s) 90, 165, 781, 889
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 2 cut(s) 66, 190
HpaII CCGG 9 cut(s) 62, 87, 132, 229, 270, 276, 327, 885, 909
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 3 cut(s) 54, 214, 547
Hpy188I TCNGA 8 cut(s) 22, 102, 155, 197, 342, 466, 474, 509
Hpy188III TCNNGA 5 cut(s) 34, 187, 487, 586, 748
Hpy8I GTNNAC 3 cut(s) 54, 214, 547
Hpy99I CGWCG 3 cut(s) 110, 338, 908
HpyAV CCTTC 5 cut(s) 474, 584, 586, 769, 808
HpyCH4III ACNGT 5 cut(s) 44, 470, 544, 757, 904
HpyCH4IV ACGT 4 cut(s) 56, 315, 336, 731
HpyCH4V TGCA 2 cut(s) 454, 697
HpyF10VI GCNNNNNNNGC 1 cut(s) 141
HpyF3I CTNAG 6 cut(s) 363, 411, 463, 506, 770, 947
HpySE526I ACGT 4 cut(s) 56, 315, 336, 731
Hsp92I GRCGYC 2 cut(s) 90, 889
Hsp92II CATG 4 cut(s) 211, 226, 447, 751
HspAI GCGC 4 cut(s) 90, 165, 781, 889
KasI GGCGCC 2 cut(s) 89, 888
KroI GCCGGC 1 cut(s) 131
KroNI GCCGGC 1 cut(s) 133
Ksp22I TGATCA 1 cut(s) 417
LmnI GCTCC 4 cut(s) 278, 604, 778, 930
Lsp1109I GCAGC 2 cut(s) 681, 867
LweI GCATC 1 cut(s) 131
MaeI CTAG 2 cut(s) 149, 560
MaeII ACGT 4 cut(s) 56, 315, 336, 731
MaeIII GTNAC 1 cut(s) 311
MbiI CCGCTC 1 cut(s) 263
MboII GAAGA 3 cut(s) 489, 813, 926
MfeI CAATTG 1 cut(s) 698
MflI RGATCY 2 cut(s) 155, 489
MhlI GDGCHC 1 cut(s) 935
MlsI TGGCCA 1 cut(s) 661
MluCI AATT 4 cut(s) 368, 655, 698, 846
MluNI TGGCCA 1 cut(s) 661
Mly113I GGCGCC 2 cut(s) 90, 889
MmeI TCCRAC 4 cut(s) 133, 183, 408, 630
Mox20I TGGCCA 1 cut(s) 661
MreI CGCCGGCG 1 cut(s) 131
MroNI GCCGGC 1 cut(s) 131
MscI TGGCCA 1 cut(s) 661
MseI TTAA 2 cut(s) 375, 867
Msp20I TGGCCA 1 cut(s) 661
MspI CCGG 9 cut(s) 62, 87, 132, 229, 270, 276, 327, 885, 909
MspR9I CCNGG 6 cut(s) 62, 63, 87, 230, 328, 886
MunI CAATTG 1 cut(s) 698
MvnI CGCG 1 cut(s) 783
MwoI GCNNNNNNNGC 1 cut(s) 141
NaeI GCCGGC 1 cut(s) 133
NarI GGCGCC 2 cut(s) 90, 889
NciI CCSGG 6 cut(s) 62, 63, 87, 230, 328, 886
NgoMIV GCCGGC 1 cut(s) 131
NheI GCTAGC 1 cut(s) 559
NlaIII CATG 4 cut(s) 211, 226, 447, 751
NlaIV GGNNCC 6 cut(s) 19, 91, 157, 274, 485, 890
NmeAIII GCCGAG 2 cut(s) 273, 856
NmuCI GTSAC 1 cut(s) 311
PagI TCATGA 1 cut(s) 747
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PdiI GCCGGC 1 cut(s) 133
PfeI GAWTC 2 cut(s) 66, 190
PkrI GCNGC 5 cut(s) 84, 309, 696, 857, 883
Ple19I CGATCG 1 cut(s) 33
PluTI GGCGCC 2 cut(s) 93, 892
Ppu21I YACGTR 1 cut(s) 57
PshBI ATTAAT 1 cut(s) 867
Psp124BI GAGCTC 1 cut(s) 935
PspFI CCCAGC 3 cut(s) 167, 218, 386
PspN4I GGNNCC 6 cut(s) 19, 91, 157, 274, 485, 890
PspPI GGNCC 4 cut(s) 226, 484, 547, 571
PsuI RGATCY 2 cut(s) 155, 489
PvuI CGATCG 1 cut(s) 33
RsaI GTAC 2 cut(s) 59, 734
RsaNI GTAC 2 cut(s) 58, 733
SacI GAGCTC 1 cut(s) 935
SalI GTCGAC 1 cut(s) 212
SaqAI TTAA 2 cut(s) 375, 867
SatI GCNGC 5 cut(s) 83, 308, 695, 856, 882
Sau96I GGNCC 4 cut(s) 226, 484, 547, 571
ScrFI CCNGG 6 cut(s) 62, 63, 87, 230, 328, 886
SduI GDGCHC 1 cut(s) 935
SfaNI GCATC 1 cut(s) 131
SfoI GGCGCC 2 cut(s) 91, 890
SgrAI CRCCGGYG 1 cut(s) 131
SinI GGWCC 4 cut(s) 226, 484, 547, 571
SmaI CCCGGG 1 cut(s) 63
SmlI CTYRAG 2 cut(s) 185, 586
SmoI CTYRAG 2 cut(s) 185, 586
Sse9I AATT 4 cut(s) 368, 655, 698, 846
SsiI CCGC 8 cut(s) 80, 83, 261, 297, 307, 398, 569, 881
SspDI GGCGCC 2 cut(s) 89, 888
SspMI CTAG 2 cut(s) 149, 560
SstI GAGCTC 1 cut(s) 935
StyD4I CCNGG 6 cut(s) 60, 61, 85, 228, 326, 884
TaaI ACNGT 5 cut(s) 44, 470, 544, 757, 904
TaiI ACGT 4 cut(s) 59, 318, 339, 734
TaqI TCGA 4 cut(s) 39, 105, 213, 333
TaqII GACCGA 2 cut(s) 153, 231
TasI AATT 4 cut(s) 368, 655, 698, 846
TauI GCSGC 3 cut(s) 85, 310, 884
TfiI GAWTC 2 cut(s) 66, 190
Tru1I TTAA 2 cut(s) 375, 867
Tru9I TTAA 2 cut(s) 375, 867
TscAI CASTG 1 cut(s) 549
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 2 cut(s) 694, 855
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 2 cut(s) 432, 855
TspMI CCCGGG 1 cut(s) 61
TspRI CASTG 1 cut(s) 549
VpaK11BI GGWCC 4 cut(s) 226, 484, 547, 571
VspI ATTAAT 1 cut(s) 867
XmaI CCCGGG 1 cut(s) 61
XmiI GTMKAC 1 cut(s) 213
XspI CTAG 2 cut(s) 149, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.