Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001214

Physical Location & Seq

Genomic Coordinates Forward (+)

7113114 .. 7114214

Gene Structure 1101 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1087100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1101 bp

ATGGCCATGCCTTCGCCGCCGCCGACGACGTCGTATTGGTGTTACAGATGCACTCGCTTCGTCCGCGTGTTTGCTCTGGACTCCGCCCTCTTGTGCCCCCACTGCGAAACCGGCTTCCTCGAAGAGATCGAGACGATGCCACAGCCGCTACTGCAGCCGAACCATCTCCGCCGTAGATTCCCGTCCTCCCACCGCCACGAGGAAGAACGGGGCCAAAACCCGACTCCGACCTCCCGCCGGGTACGACGGGCCTCCGGCGACCGGTCTTCTCTATTCAACCCGGTCATCGTCTTACGTGGCACCGAGGCCGATACCGAGTCCAACACATTCGAACTCTACTATAATGACGCCGCGGGGTCGGGGCTCCGACCACTTCCGCCCACCATGTCCGAGCTTTTAATGGGTTCGGGCTTCGACCGCTTACTTGAACAGCTCTCCCAGATTGAAATCAACGGTTTGGGCAGACCTGAAAACCCGCCTGCGTCTAAATCCGCTGTGGAATCTATGCCCGTTATTCAAATCGCCGATACCCACGTCGGGTCGGACTCGCACTGCGCCGTTTGCAAAGAAGCATTCGAACTCGGGTCGGAGGCCCGAGAAATGCCCTGCAAGCACATATACCACCCGGATTGTATCCTCCCCTGGCTCTCAATGCGTAACTCGTGCCCGGTTTGTCGACACGAGCTGCCCGCTGACCAAAACAACCGTAATTCGGATCCGGGTATCGAGGAAGAGACGATGGGTTTGACCATCTGGAGATTACCCGGCGGCGGATTTGCCGTGGGGAGATTCTCCGGCGGCAGAAGACCCGGCGAGAGGGAATTACCGGTTGTGTACACAGAAATGGACGGCGGGTTCAATGGTAACGGGGCTCCAAGAAGGGTATTATGGGCATCGAGGAGTAGAGGGAGCGAAAGTCGCGGGATTCGGAGTGCTTTTCGTAATATAGCTTCGTTCTGGGGGCGATTACGTTCCAATTCATCGTCTTCGAGGTCCGGGTCGGAATCTGAATCCGGGCCGGGTTTGACTAGAAGTCAGTCAAGTTCGGTGTTTGGGCGGTTCGTACACCGGCGTAGGAGGGCGTGGGTGTTGGATGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000209 GO:0000902 GO:0000904 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009414 GO:0009415 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009826 GO:0009888 GO:0009987 GO:0010026 GO:0010033 GO:0010035 GO:0010154 GO:0010200 GO:0010243 GO:0010498 GO:0016043 GO:0016049 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0022412 GO:0022414 GO:0030154 GO:0030163 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0032989 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0042493 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0048316 GO:0048468 GO:0048589 GO:0048608 GO:0048731 GO:0048856 GO:0048869 GO:0050896 GO:0051603 GO:0051865 GO:0060560 GO:0061458 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0071840 GO:0090376 GO:0090378 GO:0090558 GO:0097305 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1117 zf-RING_2 zinc_ribbon_9

Protein Analysis

367

Amino Acids

40.81

Weight (kDa)

8.36

Isoelectric Point (pI)

83.09

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Zn_ribbon_19	PF14369	10 - 43	3.7e-13	zinc-ribbon
zf-RING_2	PF13639	184 - 226	4.4e-12	Ring finger domain
zf-C3HC4_2	PF13923	185 - 225	1e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_11	PF17123	185 - 212	1.6e-06	RING-like zinc finger
zf-C3HC4	PF00097	185 - 225	7.6e-06	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	185 - 226	8.1e-06	RING-H2 zinc finger domain
DUF1117	PF06547	247 - 353	1.3e-36	Protein of unknown function (DUF1117)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001214)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39720 AT2G39720 AT3G46620 AT5G59550 AT5G59550
fragaria_vesca	FvH4_3g36860 FvH4_6g13340 FvH4_7g22850
malus_domestica	MD01G1136700.v1.1 MD03G1087100.v1.1 MD04G1131800.v1.1 MD07G1201700.v1.1 MD10G1074300.v1.1 MD11G1012900.v1.1 MD11G1096200.v1.1 MD12G1144900.v1.1
prunus_persica	Prupe.2G238300_v2.0.a1 Prupe.6G071800_v2.0.a1 Prupe.6G258400_v2.0.a1
pyrus_communis	pycom01g15740 pycom03g06910 pycom04g11990 pycom07g18870 pycom11g08150 pycom12g13930
rosa_chinensis	RchiOBHm_Chr1g0368011 RchiOBHm_Chr3g0466231 RchiOBHm_Chr5g0066071
rosa_laevigata	RLG00000024595 RLG00000027247 RLG00000035839
rosa_multiflora	Rmu_co8242689.1_g000001 Rmu_co8418295.1_g000001 Rmu_sc0000350.1_g000012 Rmu_sc0002406.1_g000005 Rmu_sc0005240.1_g000003 Rmu_sc0005240.1_g000004
rosa_roxburghii	Rroxscaffold_1G00014940 Rroxscaffold_4G00288940 Rroxscaffold_6G00414710
rosa_rugosa	Rorug01G0338400 Rorug03G0080500 Rorug05G0376200
rosa_samantha	Rh1AG345200 Rh1BG307500 Rh1CG323100 Rh1DG339600 Rh3BG147700 Rh3DG148400 Rh5AG434600 Rh5BG450900 Rh5CG473200 Rh5DG464500
rosa_wichuraiana	Rw1G030720 Rw3G011940 Rw5G040700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	32
AccB1I	GGYRCC	1 cut(s)	299
AccI	GTMKAC	1 cut(s)	676
AccII	CGCG	3 cut(s)	66, 353, 921
AclWI	GGATC	2 cut(s)	710, 723
AcoI	YGGCCR	1 cut(s)	3
AcyI	GRCGYC	2 cut(s)	29, 348
AfaI	GTAC	3 cut(s)	243, 836, 1065
AfiI	CCNNNNNNNGG	7 cut(s)	199, 237, 261, 537, 712, 770, 816
AgeI	ACCGGT	2 cut(s)	261, 826
AgsI	TTSAA	5 cut(s)	277, 428, 446, 518, 859
AhdI	GACNNNNNGTC	1 cut(s)	1032
AjiI	CACGTC	1 cut(s)	535
AjnI	CCWGG	1 cut(s)	641
AloI	GAACNNNNNNTCC	2 cut(s)	839, 871
AluBI	AGCT	4 cut(s)	394, 433, 685, 950
AluI	AGCT	4 cut(s)	394, 433, 685, 950
Alw26I	GTCTC	2 cut(s)	125, 728
AlwI	GGATC	2 cut(s)	710, 723
Ama87I	CYCGRG	2 cut(s)	581, 594
AoxI	GGCC	6 cut(s)	3, 211, 249, 306, 591, 1016
ApeKI	GCWGC	2 cut(s)	154, 685
ArsI	GACNNNNNNTTYG	2 cut(s)	727, 759
AsiGI	ACCGGT	2 cut(s)	261, 826
AspLEI	GCGC	1 cut(s)	557
AspS9I	GGNCC	5 cut(s)	211, 249, 592, 993, 1016
AsuII	TTCGAA	2 cut(s)	330, 576
AvaI	CYCGRG	2 cut(s)	581, 594
AvaII	GGWCC	1 cut(s)	993
BaeGI	GKGCMC	2 cut(s)	98, 668
BaeI	ACNNNNGTAYC	2 cut(s)	519, 552
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	1 cut(s)	715
BanI	GGYRCC	1 cut(s)	299
BanII	GRGCYC	2 cut(s)	366, 874
BarI	GAAGNNNNNNTAC	2 cut(s)	934, 966
BauI	CACGAG	3 cut(s)	197, 661, 680
BbsI	GAAGAC	3 cut(s)	258, 811, 978
BbvI	GCAGC	2 cut(s)	166, 672
BccI	CCATC	3 cut(s)	171, 733, 758
BceAI	ACGGC	4 cut(s)	156, 542, 764, 865
BcgI	CGANNNNNNTGC	2 cut(s)	40, 74
BciT130I	CCWGG	1 cut(s)	643
BciVI	GTATCC	1 cut(s)	644
BcoDI	GTCTC	2 cut(s)	125, 728
BfaI	CTAG	1 cut(s)	1029
BfmI	CTRYAG	1 cut(s)	152
BfuI	GTATCC	1 cut(s)	644
BisI	GCNGC	8 cut(s)	17, 20, 146, 155, 351, 686, 769, 799
BlsI	GCNGC	8 cut(s)	18, 21, 147, 156, 352, 687, 770, 800
Bme18I	GGWCC	1 cut(s)	993
BmeRI	GACNNNNNGTC	1 cut(s)	1032
BmeT110I	CYCGRG	2 cut(s)	581, 594
BmgBI	CACGTC	1 cut(s)	535
BmgT120I	GGNCC	5 cut(s)	211, 249, 592, 993, 1016
BmiI	GGNNCC	5 cut(s)	212, 301, 365, 717, 873
BmsI	GCATC	3 cut(s)	38, 126, 902
BpiI	GAAGAC	3 cut(s)	258, 811, 978
BpmI	CTGGAG	1 cut(s)	775
Bpu14I	TTCGAA	2 cut(s)	330, 576
BsaAI	YACGTR	1 cut(s)	296
BsaBI	GATNNNNATC	1 cut(s)	446
BsaHI	GRCGYC	2 cut(s)	29, 348
BsaJI	CCNNGG	4 cut(s)	303, 351, 641, 780
BsaWI	WCCGGW	2 cut(s)	261, 826
BsaXI	ACNNNNNCTCC	2 cut(s)	901, 931
Bsc4I	CCNNNNNNNGG	7 cut(s)	199, 237, 261, 537, 712, 770, 816
Bse118I	RCCGGY	4 cut(s)	110, 261, 826, 1068
Bse8I	GATNNNNATC	1 cut(s)	446
BseBI	CCWGG	1 cut(s)	643
BseDI	CCNNGG	4 cut(s)	303, 351, 641, 780
BseGI	GGATG	1 cut(s)	1099
BseJI	GATNNNNATC	1 cut(s)	446
BseLI	CCNNNNNNNGG	7 cut(s)	199, 237, 261, 537, 712, 770, 816
BseRI	GAGGAG	1 cut(s)	913
BseSI	GKGCMC	2 cut(s)	98, 668
BseXI	GCAGC	2 cut(s)	166, 672
Bsh1236I	CGCG	3 cut(s)	66, 353, 921
Bsh1285I	CGRYCG	2 cut(s)	262, 418
BshFI	GGCC	6 cut(s)	5, 213, 251, 308, 593, 1018
BshNI	GGYRCC	1 cut(s)	299
BshTI	ACCGGT	2 cut(s)	261, 826
BsiEI	CGRYCG	2 cut(s)	262, 418
BsiHKCI	CYCGRG	2 cut(s)	581, 594
BslI	CCNNNNNNNGG	7 cut(s)	199, 237, 261, 537, 712, 770, 816
BsmAI	GTCTC	2 cut(s)	125, 728
BsmBI	CGTCTC	2 cut(s)	125, 728
BsmI	GAATGC	1 cut(s)	572
BsnI	GGCC	6 cut(s)	5, 213, 251, 308, 593, 1018
BsoBI	CYCGRG	2 cut(s)	581, 594
Bsp119I	TTCGAA	2 cut(s)	330, 576
Bsp1286I	GDGCHC	4 cut(s)	98, 366, 668, 874
Bsp1407I	TGTACA	1 cut(s)	834
Bsp143I	GATC	2 cut(s)	126, 715
BspANI	GGCC	6 cut(s)	5, 213, 251, 308, 593, 1018
BspFNI	CGCG	3 cut(s)	66, 353, 921
BspLI	GGNNCC	5 cut(s)	212, 301, 365, 717, 873
BspMAI	CTGCAG	1 cut(s)	156
BspPI	GGATC	2 cut(s)	710, 723
BspT104I	TTCGAA	2 cut(s)	330, 576
BspT107I	GGYRCC	1 cut(s)	299
BsrFI	RCCGGY	4 cut(s)	110, 261, 826, 1068
BsrGI	TGTACA	1 cut(s)	834
BssAI	RCCGGY	4 cut(s)	110, 261, 826, 1068
BssECI	CCNNGG	4 cut(s)	303, 351, 641, 780
BssMI	GATC	2 cut(s)	126, 715
BssNI	GRCGYC	2 cut(s)	29, 348
BssSI	CACGAG	3 cut(s)	197, 661, 680
Bst2BI	CACGAG	3 cut(s)	197, 661, 680
Bst2UI	CCWGG	1 cut(s)	643
Bst4CI	ACNGT	2 cut(s)	455, 707
Bst6I	CTCTTC	2 cut(s)	117, 726
BstACI	GRCGYC	2 cut(s)	29, 348
BstAUI	TGTACA	1 cut(s)	834
BstBAI	YACGTR	1 cut(s)	296
BstBI	TTCGAA	2 cut(s)	330, 576
BstC8I	GCNNGC	3 cut(s)	480, 611, 690
BstDSI	CCRYGG	2 cut(s)	351, 780
BstF5I	GGATG	1 cut(s)	1099
BstFNI	CGCG	3 cut(s)	66, 353, 921
BstHHI	GCGC	1 cut(s)	557
BstKTI	GATC	2 cut(s)	129, 718
BstMAI	GTCTC	2 cut(s)	125, 728
BstMBI	GATC	2 cut(s)	126, 715
BstMCI	CGRYCG	2 cut(s)	262, 418
BstNI	CCWGG	1 cut(s)	643
BstSFI	CTRYAG	1 cut(s)	152
BstSLI	GKGCMC	2 cut(s)	98, 668
BstUI	CGCG	3 cut(s)	66, 353, 921
BstV1I	GCAGC	2 cut(s)	166, 672
BstV2I	GAAGAC	3 cut(s)	258, 811, 978
BstX2I	RGATCY	1 cut(s)	715
BstYI	RGATCY	1 cut(s)	715
BsuI	GTATCC	1 cut(s)	644
BsuRI	GGCC	6 cut(s)	5, 213, 251, 308, 593, 1018
BtgI	CCRYGG	2 cut(s)	351, 780
BtrI	CACGTC	1 cut(s)	535
BtsCI	GGATG	1 cut(s)	1099
BtsI	GCAGTG	2 cut(s)	100, 550
BtsIMutI	CAGTG	2 cut(s)	100, 550
Cac8I	GCNNGC	3 cut(s)	480, 611, 690
CfoI	GCGC	1 cut(s)	557
Cfr10I	RCCGGY	4 cut(s)	110, 261, 826, 1068
Cfr13I	GGNCC	5 cut(s)	211, 249, 592, 993, 1016
Cfr42I	CCGCGG	1 cut(s)	354
CseI	GACGC	2 cut(s)	356, 471
Csp6I	GTAC	3 cut(s)	242, 835, 1064
CspAI	ACCGGT	2 cut(s)	261, 826
CspCI	CAANNNNNGTGG	2 cut(s)	611, 646
CviAII	CATG	2 cut(s)	7, 385
CviQI	GTAC	3 cut(s)	242, 835, 1064
DpnI	GATC	2 cut(s)	128, 717
DpnII	GATC	2 cut(s)	126, 715
DriI	GACNNNNNGTC	1 cut(s)	1032
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	2 cut(s)	117, 726
Eam1105I	GACNNNNNGTC	1 cut(s)	1032
EarI	CTCTTC	2 cut(s)	117, 726
EciI	GGCGGA	4 cut(s)	73, 158, 366, 786
Eco24I	GRGCYC	2 cut(s)	366, 874
Eco47I	GGWCC	1 cut(s)	993
Eco88I	CYCGRG	2 cut(s)	581, 594
EcoRII	CCWGG	1 cut(s)	641
EcoT38I	GRGCYC	2 cut(s)	366, 874
Esp3I	CGTCTC	2 cut(s)	125, 728
FaeI	CATG	2 cut(s)	10, 388
FaiI	YATR	8 cut(s)	8, 342, 386, 506, 617, 619, 889, 947
FatI	CATG	2 cut(s)	6, 384
FauI	CCCGC	6 cut(s)	242, 346, 483, 697, 845, 914
FblI	GTMKAC	1 cut(s)	676
Fnu4HI	GCNGC	8 cut(s)	17, 20, 146, 155, 351, 686, 769, 799
FriOI	GRGCYC	2 cut(s)	366, 874
Fsp4HI	GCNGC	8 cut(s)	17, 20, 146, 155, 351, 686, 769, 799
FspBI	CTAG	1 cut(s)	1029
GlaI	GCGC	1 cut(s)	556
GluI	GCNGC	8 cut(s)	17, 20, 146, 155, 351, 686, 769, 799
GsuI	CTGGAG	1 cut(s)	775
HaeIII	GGCC	6 cut(s)	5, 213, 251, 308, 593, 1018
HgaI	GACGC	2 cut(s)	356, 471
HhaI	GCGC	1 cut(s)	557
Hin1I	GRCGYC	2 cut(s)	29, 348
Hin1II	CATG	2 cut(s)	10, 388
Hin6I	GCGC	1 cut(s)	555
HinP1I	GCGC	1 cut(s)	555
HincII	GTYRAC	1 cut(s)	677
HindII	GTYRAC	1 cut(s)	677
Hpy166II	GTNNAC	4 cut(s)	677, 835, 837, 1066
Hpy188I	TCNGA	9 cut(s)	228, 368, 391, 544, 589, 715, 930, 1003, 1009
Hpy188III	TCNNGA	3 cut(s)	77, 130, 754
Hpy8I	GTNNAC	4 cut(s)	677, 835, 837, 1066
Hpy99I	CGWCG	5 cut(s)	28, 31, 34, 249, 539
HpyAV	CCTTC	2 cut(s)	21, 873
HpyCH4III	ACNGT	2 cut(s)	455, 707
HpyCH4IV	ACGT	4 cut(s)	29, 295, 534, 970
HpyCH4V	TGCA	4 cut(s)	51, 154, 564, 609
HpySE526I	ACGT	4 cut(s)	29, 295, 534, 970
Hsp92I	GRCGYC	2 cut(s)	29, 348
Hsp92II	CATG	2 cut(s)	10, 388
HspAI	GCGC	1 cut(s)	555
KspI	CCGCGG	1 cut(s)	354
Kzo9I	GATC	2 cut(s)	126, 715
LmnI	GCTCC	3 cut(s)	369, 877, 909
Lsp1109I	GCAGC	2 cut(s)	166, 672
LweI	GCATC	3 cut(s)	38, 126, 902
MaeI	CTAG	1 cut(s)	1029
MaeII	ACGT	4 cut(s)	29, 295, 534, 970
MaeIII	GTNAC	3 cut(s)	41, 656, 863
MalI	GATC	2 cut(s)	128, 717
MboI	GATC	2 cut(s)	126, 715
MboII	GAAGA	6 cut(s)	134, 215, 258, 743, 816, 978
MflI	RGATCY	1 cut(s)	715
MhlI	GDGCHC	4 cut(s)	98, 366, 668, 874
MlsI	TGGCCA	1 cut(s)	5
MluCI	AATT	3 cut(s)	709, 821, 976
MluNI	TGGCCA	1 cut(s)	5
MlyI	GAGTC	4 cut(s)	74, 217, 326, 539
MmeI	TCCRAC	7 cut(s)	251, 345, 391, 522, 567, 981, 1071
Mox20I	TGGCCA	1 cut(s)	5
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	1 cut(s)	398
MslI	CAYNNNNRTG	1 cut(s)	842
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	3 cut(s)	353, 494, 692
Mva1269I	GAATGC	1 cut(s)	572
MvaI	CCWGG	1 cut(s)	643
MvnI	CGCG	3 cut(s)	66, 353, 921
NdeII	GATC	2 cut(s)	126, 715
NlaIII	CATG	2 cut(s)	10, 388
NlaIV	GGNNCC	5 cut(s)	212, 301, 365, 717, 873
NspV	TTCGAA	2 cut(s)	330, 576
PcsI	WCGNNNNNNNCGW	2 cut(s)	20, 126
PctI	GAATGC	1 cut(s)	572
PfeI	GAWTC	6 cut(s)	177, 500, 789, 925, 1004, 1010
PflFI	GACNNNGTC	1 cut(s)	28
PinAI	ACCGGT	2 cut(s)	261, 826
PkrI	GCNGC	8 cut(s)	18, 21, 147, 156, 352, 687, 770, 800
PleI	GAGTC	4 cut(s)	74, 217, 325, 539
PpsI	GAGTC	4 cut(s)	74, 217, 325, 539
Ppu21I	YACGTR	1 cut(s)	296
Psp6I	CCWGG	1 cut(s)	641
PspGI	CCWGG	1 cut(s)	641
PspN4I	GGNNCC	5 cut(s)	212, 301, 365, 717, 873
PspPI	GGNCC	5 cut(s)	211, 249, 592, 993, 1016
PstI	CTGCAG	1 cut(s)	156
PsuI	RGATCY	1 cut(s)	715
PsyI	GACNNNGTC	1 cut(s)	28
RsaI	GTAC	3 cut(s)	243, 836, 1065
RsaNI	GTAC	3 cut(s)	242, 835, 1064
RseI	CAYNNNNRTG	1 cut(s)	842
SacII	CCGCGG	1 cut(s)	354
SalI	GTCGAC	1 cut(s)	675
SaqAI	TTAA	1 cut(s)	398
SatI	GCNGC	8 cut(s)	17, 20, 146, 155, 351, 686, 769, 799
Sau3AI	GATC	2 cut(s)	126, 715
Sau96I	GGNCC	5 cut(s)	211, 249, 592, 993, 1016
SchI	GAGTC	4 cut(s)	74, 217, 326, 539
SduI	GDGCHC	4 cut(s)	98, 366, 668, 874
SfaNI	GCATC	3 cut(s)	38, 126, 902
SfcI	CTRYAG	1 cut(s)	152
Sfr303I	CCGCGG	1 cut(s)	354
SfuI	TTCGAA	2 cut(s)	330, 576
SgrAI	CRCCGGYG	1 cut(s)	1068
SgrBI	CCGCGG	1 cut(s)	354
SinI	GGWCC	1 cut(s)	993
SmiMI	CAYNNNNRTG	1 cut(s)	842
Sse9I	AATT	3 cut(s)	709, 821, 976
SspMI	CTAG	1 cut(s)	1029
TaaI	ACNGT	2 cut(s)	455, 707
TaiI	ACGT	4 cut(s)	32, 298, 537, 973
TaqI	TCGA	9 cut(s)	120, 129, 330, 414, 576, 676, 726, 896, 989
TasI	AATT	3 cut(s)	709, 821, 976
TatI	WGTACW	1 cut(s)	834
TauI	GCSGC	6 cut(s)	19, 22, 148, 353, 771, 801
TfiI	GAWTC	6 cut(s)	177, 500, 789, 925, 1004, 1010
Tru1I	TTAA	1 cut(s)	398
Tru9I	TTAA	1 cut(s)	398
TscAI	CASTG	2 cut(s)	107, 557
TseI	GCWGC	2 cut(s)	154, 685
TspDTI	ATGAA	1 cut(s)	969
TspRI	CASTG	2 cut(s)	107, 557
Tth111I	GACNNNGTC	1 cut(s)	28
VpaK11BI	GGWCC	1 cut(s)	993
XmiI	GTMKAC	1 cut(s)	676
XspI	CTAG	1 cut(s)	1029
ZraI	GACGTC	1 cut(s)	30

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.