pycom12g13930

E3 ubiquitin-protein ligase RING1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001214
Physical Location & Seq
Reverse (-)
16393029 .. 16394096
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g13930.1

Sequence Viewer

Length: 1068 bp
ATGTCTTCCATGGCCGCATCGTCCTACTGGTGCTACACGTGTAACCGGTCGATTCATGTTAGGGTTCGGACCCAAGATTCGATCCTTTGCCCCGATTGCGGAGCTGGGTTTATTGAAGAGATCCCGACCCCGACCCGATCGTCTCCGGTCCACCACCCGTTTCCCACCGCCGCGATGTTCCTTGACAGCGCCCCGGCTTCGCAGAGTCCGAGCCCGCCTATACTTCGCCGGAATCAGAGAAGCGGCCGCGATCGGTCCCCGTTTAACCCGGTCATCGTTCTTCGGGGCCCACCCGACACAGAATCCGACCCGGGAAGTTTCGAGCTGTTCTATGACGATGGAGCTGGGATGGGTCTCCGCCCGCTGCCGTCGTCGATGTCGGATATCTTAATGGGTTCGGGTTTCGACCGGTTGCTCCACCAGTTGACCCAATTGGAAAACGGTGTCGCGCGGCTCGACCACCCTCCGGCGTCCAAAGCCGCGATTGAGTCGATGCCCGTCATCAAAATCGCCGACAGCCATGTCTCGACGGAGTTGCACTGCGCCGTTTGCAAAGAGGCTTTCGAGCTCGACTCTGAAGCGCGGGAGATGCCGTGTAAGCACATCTACCACTCAGATTGTATCTTCCCATGGCTTTCGATCCGCAATTCGTGCCCGGTTTGCCGGTACCAGCTCCCCACCGATGTGAGAGGCCCGCGTGGCAGTGGCATGGCTTCGCCAGAGAACAATGGCGTGGTTGGAGAAGAGGAGACAGTGGGGTTGACTATCTGGAGATTGCCCGGTGGCGGATTCGCTGTGGGGAGGTTCACCGGAGGCAGAAGAGCCGCGGAGCGCGAGCTTCCAGTTGTTTACACCGAGATGGATGGTGGGTTCAATACGGCCGGTGCTCCTAGGAGGATTTCATGGGCTTCTAGAAGAAGGGCTAGAGAGAGTGGGGGGTTCGCTCAGGCCTTTCGCAATTTCTTCTCTTTTTTCGGCAGGCTGAGAAGCTCGAGAAGTCGGTCTGGCAATGTGTTCAGTAGACCATCACGGAGGAGGAGCCAGCGTTGGGCTTTGGAAGACAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1117 zf-RING_2 zinc_ribbon_9

Protein Analysis

356

Amino Acids

39.23

Weight (kDa)

8.88

Isoelectric Point (pI)

81.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 7 - 41 3.5e-14 zinc-ribbon
zf-RING_2 PF13639 180 - 222 5.6e-12 Ring finger domain
zf-rbx1 PF12678 180 - 222 4.3e-06 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 181 - 221 3.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 181 - 221 9.8e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 181 - 208 2.9e-06 RING-like zinc finger
DUF1117 PF06547 252 - 348 2.3e-38 Protein of unknown function (DUF1117)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 139, 521
Acc65I GGTACC 1 cut(s) 666
AccB1I GGYRCC 1 cut(s) 666
AccI GTMKAC 1 cut(s) 1021
AccII CGCG 9 cut(s) 173, 249, 449, 451, 482, 583, 697, 827, 834
AclWI GGATC 3 cut(s) 76, 115, 634
AcoI YGGCCR 3 cut(s) 12, 244, 879
AcuI CTGAAG 1 cut(s) 597
AcvI CACGTG 1 cut(s) 39
AcyI GRCGYC 1 cut(s) 470
AfaI GTAC 1 cut(s) 668
AfiI CCNNNNNNNGG 4 cut(s) 98, 466, 785, 1048
AflIII ACRYGT 2 cut(s) 36, 38
AgeI ACCGGT 2 cut(s) 45, 408
AgsI TTSAA 2 cut(s) 116, 874
AleI CACNNNNGTG 1 cut(s) 683
AloI GAACNNNNNNTCC 2 cut(s) 854, 886
AluBI AGCT 7 cut(s) 104, 325, 344, 568, 673, 838, 990
AluI AGCT 7 cut(s) 104, 325, 344, 568, 673, 838, 990
Alw21I GWGCWC 2 cut(s) 570, 889
Alw26I GTCTC 4 cut(s) 147, 359, 529, 743
AlwI GGATC 3 cut(s) 76, 115, 634
Ama87I CYCGRG 2 cut(s) 310, 991
AoxI GGCC 6 cut(s) 12, 244, 286, 691, 879, 948
ApaI GGGCCC 1 cut(s) 290
ApeKI GCWGC 1 cut(s) 364
ArsI GACNNNNNNTTYG 2 cut(s) 61, 93
AsiGI ACCGGT 2 cut(s) 45, 408
Asp718I GGTACC 1 cut(s) 666
AspA2I CCTAGG 1 cut(s) 890
AspLEI GCGC 5 cut(s) 191, 451, 545, 583, 834
AspS9I GGNCC 6 cut(s) 69, 148, 255, 286, 287, 692
AsuC2I CCSGG 6 cut(s) 194, 269, 311, 312, 656, 780
AsuHPI GGTGA 1 cut(s) 799
AvaI CYCGRG 2 cut(s) 310, 991
AvaII GGWCC 3 cut(s) 69, 148, 255
AvrII CCTAGG 1 cut(s) 890
BaeGI GKGCMC 2 cut(s) 290, 656
BanI GGYRCC 1 cut(s) 666
BanII GRGCYC 3 cut(s) 215, 290, 570
BbrPI CACGTG 1 cut(s) 39
Bbv12I GWGCWC 2 cut(s) 570, 889
BbvI GCAGC 1 cut(s) 351
BccI CCATC 5 cut(s) 332, 343, 853, 857, 1033
BceAI ACGGC 4 cut(s) 352, 530, 577, 894
BcgI CGANNNNNNTGC 2 cut(s) 517, 551
BcnI CCSGG 6 cut(s) 194, 269, 311, 312, 656, 780
BcoDI GTCTC 4 cut(s) 147, 359, 529, 743
BfaI CTAG 3 cut(s) 891, 912, 924
BfoI RGCGCY 1 cut(s) 192
BglI GCCNNNNNGGC 1 cut(s) 699
BisI GCNGC 8 cut(s) 15, 171, 244, 247, 365, 452, 480, 825
BlnI CCTAGG 1 cut(s) 890
BlsI GCNGC 8 cut(s) 16, 172, 245, 248, 366, 453, 481, 826
Bme1390I CCNGG 6 cut(s) 194, 269, 311, 312, 656, 780
Bme18I GGWCC 3 cut(s) 69, 148, 255
BmeT110I CYCGRG 2 cut(s) 310, 991
BmgT120I GGNCC 6 cut(s) 69, 148, 255, 286, 287, 692
BmiI GGNNCC 6 cut(s) 71, 257, 287, 288, 668, 1040
BmrFI CCNGG 6 cut(s) 194, 269, 311, 312, 656, 780
BmsI GCATC 3 cut(s) 26, 483, 579
BplI GAGNNNNNCTC 2 cut(s) 557, 589
BpmI CTGGAG 1 cut(s) 790
Bpu10I CCTNAGC 1 cut(s) 945
BpuMI CCSGG 6 cut(s) 194, 269, 311, 312, 656, 780
BsaAI YACGTR 1 cut(s) 39
BsaHI GRCGYC 1 cut(s) 470
BsaI GGTCTC 1 cut(s) 359
BsaJI CCNNGG 6 cut(s) 9, 192, 310, 629, 825, 890
BsaWI WCCGGW 4 cut(s) 45, 145, 408, 809
BsaXI ACNNNNNCTCC 4 cut(s) 578, 608, 1030, 1060
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 466, 785, 1048
Bse118I RCCGGY 4 cut(s) 45, 408, 663, 881
Bse1I ACTGG 3 cut(s) 32, 421, 842
Bse3DI GCAATG 1 cut(s) 1015
BseDI CCNNGG 6 cut(s) 9, 192, 310, 629, 825, 890
BseGI GGATG 2 cut(s) 354, 868
BseLI CCNNNNNNNGG 4 cut(s) 98, 466, 785, 1048
BseMI GCAATG 1 cut(s) 1015
BseMII CTCAG 3 cut(s) 627, 959, 974
BseNI ACTGG 3 cut(s) 32, 421, 842
BseRI GAGGAG 3 cut(s) 761, 1048, 1051
BseSI GKGCMC 2 cut(s) 290, 656
BseX3I CGGCCG 2 cut(s) 244, 879
BseXI GCAGC 1 cut(s) 351
BseYI CCCAGC 2 cut(s) 104, 344
Bsh1236I CGCG 9 cut(s) 173, 249, 449, 451, 482, 583, 697, 827, 834
Bsh1285I CGRYCG 6 cut(s) 50, 140, 247, 253, 409, 882
BshFI GGCC 6 cut(s) 14, 246, 288, 693, 881, 950
BshNI GGYRCC 1 cut(s) 666
BshTI ACCGGT 2 cut(s) 45, 408
BsiEI CGRYCG 6 cut(s) 50, 140, 247, 253, 409, 882
BsiHKAI GWGCWC 2 cut(s) 570, 889
BsiHKCI CYCGRG 2 cut(s) 310, 991
BslFI GGGAC 1 cut(s) 241
BslI CCNNNNNNNGG 4 cut(s) 98, 466, 785, 1048
BsmAI GTCTC 4 cut(s) 147, 359, 529, 743
BsmBI CGTCTC 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 241
BsnI GGCC 6 cut(s) 14, 246, 288, 693, 881, 950
Bso31I GGTCTC 1 cut(s) 359
BsoBI CYCGRG 2 cut(s) 310, 991
Bsp120I GGGCCC 1 cut(s) 286
Bsp1286I GDGCHC 5 cut(s) 215, 290, 570, 656, 889
Bsp143I GATC 5 cut(s) 81, 120, 137, 250, 639
Bsp19I CCATGG 2 cut(s) 9, 629
BspANI GGCC 6 cut(s) 14, 246, 288, 693, 881, 950
BspCNI CTCAG 3 cut(s) 626, 958, 975
BspFNI CGCG 9 cut(s) 173, 249, 449, 451, 482, 583, 697, 827, 834
BspLI GGNNCC 6 cut(s) 71, 257, 287, 288, 668, 1040
BspPI GGATC 3 cut(s) 76, 115, 634
BspQI GCTCTTC 1 cut(s) 814
BspT107I GGYRCC 1 cut(s) 666
BspTNI GGTCTC 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 1015
BsrFI RCCGGY 4 cut(s) 45, 408, 663, 881
BsrI ACTGG 3 cut(s) 32, 421, 842
BssAI RCCGGY 4 cut(s) 45, 408, 663, 881
BssECI CCNNGG 6 cut(s) 9, 192, 310, 629, 825, 890
BssMI GATC 5 cut(s) 81, 120, 137, 250, 639
BssNI GRCGYC 1 cut(s) 470
BssT1I CCWWGG 3 cut(s) 9, 629, 890
Bst4CI ACNGT 2 cut(s) 443, 754
Bst6I CTCTTC 3 cut(s) 111, 738, 814
BstACI GRCGYC 1 cut(s) 470
BstAPI GCANNNNNTGC 1 cut(s) 651
BstBAI YACGTR 1 cut(s) 39
BstC8I GCNNGC 6 cut(s) 215, 362, 695, 836, 980, 1043
BstDEI CTNAG 3 cut(s) 613, 945, 983
BstDSI CCRYGG 3 cut(s) 9, 629, 825
BstF5I GGATG 2 cut(s) 354, 868
BstFNI CGCG 9 cut(s) 173, 249, 449, 451, 482, 583, 697, 827, 834
BstH2I RGCGCY 1 cut(s) 192
BstHHI GCGC 5 cut(s) 191, 451, 545, 583, 834
BstKTI GATC 5 cut(s) 84, 123, 140, 253, 642
BstMAI GTCTC 4 cut(s) 147, 359, 529, 743
BstMBI GATC 5 cut(s) 81, 120, 137, 250, 639
BstMCI CGRYCG 6 cut(s) 50, 140, 247, 253, 409, 882
BstMWI GCNNNNNNNGC 8 cut(s) 96, 476, 549, 589, 598, 651, 660, 699
BstSCI CCNGG 6 cut(s) 192, 267, 309, 310, 654, 778
BstSLI GKGCMC 2 cut(s) 290, 656
BstUI CGCG 9 cut(s) 173, 249, 449, 451, 482, 583, 697, 827, 834
BstV1I GCAGC 1 cut(s) 351
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
BstZI CGGCCG 2 cut(s) 244, 879
BsuRI GGCC 6 cut(s) 14, 246, 288, 693, 881, 950
BtgI CCRYGG 3 cut(s) 9, 629, 825
BtgZI GCGATG 1 cut(s) 188
BtsCI GGATG 2 cut(s) 354, 868
BtsI GCAGTG 2 cut(s) 538, 709
BtsIMutI CAGTG 3 cut(s) 538, 709, 759
Cac8I GCNNGC 6 cut(s) 215, 362, 695, 836, 980, 1043
CciNI GCGGCCGC 1 cut(s) 244
CfoI GCGC 5 cut(s) 191, 451, 545, 583, 834
Cfr10I RCCGGY 4 cut(s) 45, 408, 663, 881
Cfr13I GGNCC 6 cut(s) 69, 148, 255, 286, 287, 692
Cfr42I CCGCGG 1 cut(s) 828
Cfr9I CCCGGG 1 cut(s) 310
CseI GACGC 1 cut(s) 459
Csp6I GTAC 1 cut(s) 667
CspAI ACCGGT 2 cut(s) 45, 408
CspCI CAANNNNNGTGG 2 cut(s) 599, 634
CviAII CATG 6 cut(s) 10, 56, 521, 630, 709, 903
CviQI GTAC 1 cut(s) 667
DdeI CTNAG 3 cut(s) 613, 945, 983
DpnI GATC 5 cut(s) 83, 122, 139, 252, 641
DpnII GATC 5 cut(s) 81, 120, 137, 250, 639
DrdI GACNNNNNNGTC 2 cut(s) 139, 521
DseDI GACNNNNNNGTC 2 cut(s) 139, 521
EaeI YGGCCR 3 cut(s) 12, 244, 879
EagI CGGCCG 2 cut(s) 244, 879
Eam1104I CTCTTC 3 cut(s) 111, 738, 814
EarI CTCTTC 3 cut(s) 111, 738, 814
EciI GGCGGA 2 cut(s) 347, 801
Ecl136II GAGCTC 1 cut(s) 568
EclXI CGGCCG 2 cut(s) 244, 879
Eco130I CCWWGG 3 cut(s) 9, 629, 890
Eco147I AGGCCT 1 cut(s) 950
Eco24I GRGCYC 3 cut(s) 215, 290, 570
Eco31I GGTCTC 1 cut(s) 359
Eco32I GATATC 1 cut(s) 385
Eco47I GGWCC 3 cut(s) 69, 148, 255
Eco52I CGGCCG 2 cut(s) 244, 879
Eco53kI GAGCTC 1 cut(s) 568
Eco57I CTGAAG 1 cut(s) 597
Eco72I CACGTG 1 cut(s) 39
Eco88I CYCGRG 2 cut(s) 310, 991
EcoICRI GAGCTC 1 cut(s) 568
EcoO109I RGGNCCY 1 cut(s) 286
EcoRV GATATC 1 cut(s) 385
EcoT14I CCWWGG 3 cut(s) 9, 629, 890
EcoT38I GRGCYC 3 cut(s) 215, 290, 570
ErhI CCWWGG 3 cut(s) 9, 629, 890
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 6 cut(s) 13, 59, 524, 633, 712, 906
FaiI YATR 8 cut(s) 11, 57, 221, 333, 522, 631, 710, 904
FaqI GGGAC 1 cut(s) 241
FatI CATG 6 cut(s) 9, 55, 520, 629, 708, 902
FauI CCCGC 4 cut(s) 222, 369, 576, 702
FblI GTMKAC 1 cut(s) 1021
Fnu4HI GCNGC 8 cut(s) 15, 171, 244, 247, 365, 452, 480, 825
FokI GGATG 2 cut(s) 361, 875
FriOI GRGCYC 3 cut(s) 215, 290, 570
Fsp4HI GCNGC 8 cut(s) 15, 171, 244, 247, 365, 452, 480, 825
FspBI CTAG 3 cut(s) 891, 912, 924
GlaI GCGC 5 cut(s) 190, 450, 544, 582, 833
GluI GCNGC 8 cut(s) 15, 171, 244, 247, 365, 452, 480, 825
GsaI CCCAGC 2 cut(s) 108, 348
GsuI CTGGAG 1 cut(s) 790
HaeII RGCGCY 1 cut(s) 192
HaeIII GGCC 6 cut(s) 14, 246, 288, 693, 881, 950
HgaI GACGC 1 cut(s) 459
HhaI GCGC 5 cut(s) 191, 451, 545, 583, 834
Hin1I GRCGYC 1 cut(s) 470
Hin1II CATG 6 cut(s) 13, 59, 524, 633, 712, 906
Hin6I GCGC 5 cut(s) 189, 449, 543, 581, 832
HinP1I GCGC 5 cut(s) 189, 449, 543, 581, 832
HincII GTYRAC 2 cut(s) 426, 762
HindII GTYRAC 2 cut(s) 426, 762
HinfI GANTC 8 cut(s) 52, 77, 205, 232, 302, 488, 572, 789
HphI GGTGA 1 cut(s) 799
Hpy166II GTNNAC 6 cut(s) 151, 426, 762, 807, 850, 1022
Hpy188I TCNGA 7 cut(s) 69, 210, 237, 307, 382, 577, 616
Hpy188III TCNNGA 5 cut(s) 124, 526, 769, 912, 993
Hpy8I GTNNAC 6 cut(s) 151, 426, 762, 807, 850, 1022
Hpy99I CGWCG 3 cut(s) 373, 376, 532
HpyAV CCTTC 1 cut(s) 912
HpyCH4III ACNGT 2 cut(s) 443, 754
HpyCH4IV ACGT 1 cut(s) 38
HpyCH4V TGCA 2 cut(s) 538, 552
HpyF10VI GCNNNNNNNGC 8 cut(s) 96, 476, 549, 589, 598, 651, 660, 699
HpyF3I CTNAG 3 cut(s) 613, 945, 983
HpySE526I ACGT 1 cut(s) 38
Hsp92I GRCGYC 1 cut(s) 470
Hsp92II CATG 6 cut(s) 13, 59, 524, 633, 712, 906
HspAI GCGC 5 cut(s) 189, 449, 543, 581, 832
KpnI GGTACC 1 cut(s) 670
KspI CCGCGG 1 cut(s) 828
Kzo9I GATC 5 cut(s) 81, 120, 137, 250, 639
LguI GCTCTTC 1 cut(s) 814
LmnI GCTCC 7 cut(s) 101, 341, 420, 678, 829, 892, 1038
Lsp1109I GCAGC 1 cut(s) 351
LweI GCATC 3 cut(s) 26, 483, 579
MaeI CTAG 3 cut(s) 891, 912, 924
MaeII ACGT 1 cut(s) 38
MaeIII GTNAC 1 cut(s) 41
MalI GATC 5 cut(s) 83, 122, 139, 252, 641
MboI GATC 5 cut(s) 81, 120, 137, 250, 639
MboII GAAGA 7 cut(s) 128, 272, 616, 755, 831, 927, 955
MfeI CAATTG 1 cut(s) 431
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 5 cut(s) 215, 290, 570, 656, 889
MluCI AATT 3 cut(s) 431, 646, 958
MlyI GAGTC 3 cut(s) 214, 497, 566
MmeI TCCRAC 3 cut(s) 330, 360, 718
MnlI CCTC 9 cut(s) 474, 550, 683, 739, 795, 806, 888, 1026, 1029
MseI TTAA 2 cut(s) 264, 389
MslI CAYNNNNRTG 2 cut(s) 683, 857
MspA1I CMGCKG 2 cut(s) 364, 827
MspR9I CCNGG 6 cut(s) 194, 269, 311, 312, 656, 780
MunI CAATTG 1 cut(s) 431
MvnI CGCG 9 cut(s) 173, 249, 449, 451, 482, 583, 697, 827, 834
MwoI GCNNNNNNNGC 8 cut(s) 96, 476, 549, 589, 598, 651, 660, 699
NciI CCSGG 6 cut(s) 194, 269, 311, 312, 656, 780
NcoI CCATGG 2 cut(s) 9, 629
NdeII GATC 5 cut(s) 81, 120, 137, 250, 639
NlaIII CATG 6 cut(s) 13, 59, 524, 633, 712, 906
NlaIV GGNNCC 6 cut(s) 71, 257, 287, 288, 668, 1040
NotI GCGGCCGC 1 cut(s) 244
OliI CACNNNNGTG 1 cut(s) 683
PaeR7I CTCGAG 1 cut(s) 991
PceI AGGCCT 1 cut(s) 950
PciSI GCTCTTC 1 cut(s) 814
PcsI WCGNNNNNNNCGW 2 cut(s) 206, 453
PfeI GAWTC 5 cut(s) 52, 77, 232, 302, 789
PinAI ACCGGT 2 cut(s) 45, 408
PkrI GCNGC 8 cut(s) 16, 172, 245, 248, 366, 453, 481, 826
Ple19I CGATCG 2 cut(s) 140, 253
PleI GAGTC 3 cut(s) 213, 496, 566
PmaCI CACGTG 1 cut(s) 39
PmlI CACGTG 1 cut(s) 39
PpsI GAGTC 3 cut(s) 213, 496, 566
Ppu21I YACGTR 1 cut(s) 39
Psp124BI GAGCTC 1 cut(s) 570
PspCI CACGTG 1 cut(s) 39
PspFI CCCAGC 2 cut(s) 104, 344
PspN4I GGNNCC 6 cut(s) 71, 257, 287, 288, 668, 1040
PspOMI GGGCCC 1 cut(s) 286
PspPI GGNCC 6 cut(s) 69, 148, 255, 286, 287, 692
PsuI RGATCY 1 cut(s) 120
PvuI CGATCG 2 cut(s) 140, 253
RsaI GTAC 1 cut(s) 668
RsaNI GTAC 1 cut(s) 667
RseI CAYNNNNRTG 2 cut(s) 683, 857
SacI GAGCTC 1 cut(s) 570
SacII CCGCGG 1 cut(s) 828
SapI GCTCTTC 1 cut(s) 814
SaqAI TTAA 2 cut(s) 264, 389
SatI GCNGC 8 cut(s) 15, 171, 244, 247, 365, 452, 480, 825
Sau3AI GATC 5 cut(s) 81, 120, 137, 250, 639
Sau96I GGNCC 6 cut(s) 69, 148, 255, 286, 287, 692
SchI GAGTC 3 cut(s) 214, 497, 566
ScrFI CCNGG 6 cut(s) 194, 269, 311, 312, 656, 780
SduI GDGCHC 5 cut(s) 215, 290, 570, 656, 889
SetI ASST 9 cut(s) 41, 106, 327, 346, 570, 675, 806, 840, 992
SfaNI GCATC 3 cut(s) 26, 483, 579
Sfr274I CTCGAG 1 cut(s) 991
Sfr303I CCGCGG 1 cut(s) 828
SgrBI CCGCGG 1 cut(s) 828
SinI GGWCC 3 cut(s) 69, 148, 255
SlaI CTCGAG 1 cut(s) 991
SmaI CCCGGG 1 cut(s) 312
SmiMI CAYNNNNRTG 2 cut(s) 683, 857
SmlI CTYRAG 1 cut(s) 991
SmoI CTYRAG 1 cut(s) 991
Sse9I AATT 3 cut(s) 431, 646, 958
SseBI AGGCCT 1 cut(s) 950
SspMI CTAG 3 cut(s) 891, 912, 924
SstI GAGCTC 1 cut(s) 570
StuI AGGCCT 1 cut(s) 950
StyD4I CCNGG 6 cut(s) 192, 267, 309, 310, 654, 778
StyI CCWWGG 3 cut(s) 9, 629, 890
TaaI ACNGT 2 cut(s) 443, 754
TaiI ACGT 1 cut(s) 41
TaqII GACCGA 2 cut(s) 243, 990
TasI AATT 3 cut(s) 431, 646, 958
TauI GCSGC 7 cut(s) 17, 173, 246, 249, 454, 482, 827
TfiI GAWTC 5 cut(s) 52, 77, 232, 302, 789
Tru1I TTAA 2 cut(s) 264, 389
Tru9I TTAA 2 cut(s) 264, 389
TscAI CASTG 3 cut(s) 545, 709, 759
TseI GCWGC 1 cut(s) 364
TspDTI ATGAA 2 cut(s) 44, 891
TspGWI ACGGA 2 cut(s) 545, 1045
TspMI CCCGGG 1 cut(s) 310
TspRI CASTG 3 cut(s) 545, 709, 759
VpaK11BI GGWCC 3 cut(s) 69, 148, 255
XbaI TCTAGA 1 cut(s) 911
XhoI CTCGAG 1 cut(s) 991
XmaI CCCGGG 1 cut(s) 310
XmaJI CCTAGG 1 cut(s) 890
XmiI GTMKAC 1 cut(s) 1021
XspI CTAG 3 cut(s) 891, 912, 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.