Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0002405

Physical Location & Seq

Genomic Coordinates Forward (+)

26845577 .. 26847711

Gene Structure 2135 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1163600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1344 bp

ATGGAAAATAAGGGCAAGATTTTGATGCAAAAATATGAGATTGGGAGAATGTTAGGGCAAGGAAACTTTGCTAAAGTTTATCATGCAAGAAACCTCCAAACTGGCCATAGCGTGGCGATTAAGGTAATTAACAAAGAAAAGGTTCTGAAAGTTGGACTAATTGATCAAACAAAAAGAGAGATTTCTGTTATGAGACTCGTTAAACACCCGAATATAGTGCAACTTTTTGAGGTCATGGCTACGAAAACCAAGATTTATTTTGTTTTAGAGTATGTCAAAGGCGGCGAGCTTTTCCACAAGGTGTCGAAAGGGAGGCTCAAGGAGGAAATGGCAAGGAAGTACTTTCAGCAGCTGATCAGCGCTGTGGATTTCTGCCACAGCAGAGGTGTTTACCACCGCGATTTGAAGCCTGAGAACTTGCTGCTGGACGAAAATGGAACGCTGAAGGTGTCGGATTTTGGGTTGAGTGCGCTTGCGGAATCCAACAGGCAAGATGGTCTTTTGCACACAACTTGTGGAACTCCTGCTTATGTAGCTCCTGAAGTTGTGGGGAGAAAAGCATATGATGGGGCAAAAGCTGACATTTGGTCTTGTGGTGTGATTTTGTACGTGCTTTTGGCCGGTTATCTGCCGTTTCATGGAACAAATCTGATACAAATGTATAGGAAAATTGGAAGAGCAGATTACGAATGCCCTAAGTGGTTTTCGAATGAAGCGCGACGGCTACTCTCTAAAATCCTCAACCCTAACCCTAGTAAAAGGATTTTCATTTCAAACATAATGCAAAATCCCTGGTTTAGCAAGGGGTTCAACTCCAAACCAATTGCAACTATAACAGAAGTCGGAAGCCAGGGTTTTTCGGACGTTGATCAGATTACTGAGCTGGCAAAGCAGCAAGAGTTGGCTAGACCTAGACCTACATATTTAAATGCTTTTGATATCATCTCTCTTTCTGGTGGATTTGATTTGTCTGGCTTGTTTATGGGACAGGAACAAGATGTGAAAAAAGAAGTGAAGTTCACATCTGTGCAATCTGCCTCATCAATTGTATCCAAGCTGGAGAACATTGCTCATCATTTGAAGTTGAAAGTCAAGAAAGATGGAGGGTTACTGAAATTCGAGAAATCTGATAACGGAAGGAAGGGTGCATTGGCAATAGAGGCTGAGATTTTTGAGTTCACTTCATCTTTTCATTTAATGAAGAAATCCAAAGGCGACACATTGGAATTTAGGAAGGTGTTGGAAGATGATATAAGACCAGCTCTCAAGGATGTTGTGTGGACATGGCACGGTGAGCAGAAACAAGAACAAGAACAAAAACAGCATATGCATTATTCTTCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018107 GO:0018193 GO:0018209 GO:0018210 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04068 ko04113 ko04138 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04113 map04138 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

448

Amino Acids

50.73

Weight (kDa)

9.4

Isoelectric Point (pI)

32.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	12 - 266	5.8e-78	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	13 - 262	3.9e-40	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	101 - 252	1.1e-06	Kinase-like
NAF	PF03822	306 - 366	4.8e-21	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002405)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g25640
malus_domestica	MD01G1163600.v1.1 MD02G1008400.v1.1
prunus_persica	Prupe.2G261600_v2.0.a1 Prupe.2G261600_v2.0.a1
pyrus_communis	pycom10g23210
rosa_chinensis	RchiOBHm_Chr1g0371771 RchiOBHm_Chr1g0371781 RchiOBHm_Chr1g0371791
rosa_laevigata	RLG00000011059 RLG00000016229 RLG00000018596 RLG00000026588 RLG00000026589 RLG00000026941 RLG00000026942
rosa_multiflora	Rmu_co8130288.1_g000001 Rmu_co8250369.1_g000001 Rmu_sc0001755.1_g000050 Rmu_sc0001755.1_g000051 Rmu_sc0002002.1_g000024 Rmu_sc0003033.1_g000017
rosa_roxburghii	Rroxscaffold_1G00037910 Rroxscaffold_1G00037920 Rroxscaffold_1G00037930 Rroxscaffold_1G00037940 Rroxscaffold_1G00037950 Rroxscaffold_1G00037960 Rroxscaffold_1G00037970 Rroxscaffold_4G00285430 Rroxscaffold_4G00301130 Rroxscaffold_4G00301140
rosa_rugosa	Rorug01G0366400 Rorug01G0366500 Rorug02G0441700
rosa_samantha	Rh1AG376300 Rh1AG376400 Rh1AG376500 Rh1BG339700 Rh1BG339800
rosa_wichuraiana	Rw1G033110 Rw1G033120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	112
AccII	CGCG	2 cut(s)	399, 718
AciI	CCGC	3 cut(s)	282, 397, 476
AcoI	YGGCCR	2 cut(s)	103, 618
AcsI	RAATTY	2 cut(s)	1115, 1226
AcuI	CTGAAG	2 cut(s)	464, 561
AdeI	CACNNNGTG	1 cut(s)	301
AfaI	GTAC	2 cut(s)	341, 608
AfeI	AGCGCT	1 cut(s)	361
AfiI	CCNNNNNNNGG	2 cut(s)	112, 638
AgsI	TTSAA	5 cut(s)	406, 774, 811, 1081, 1087
AhdI	GACNNNNNGTC	1 cut(s)	586
AjnI	CCWGG	2 cut(s)	791, 849
AjuI	GAANNNNNNNTTGG	2 cut(s)	1133, 1165
AleI	CACNNNNGTG	1 cut(s)	1025
AluBI	AGCT	7 cut(s)	289, 352, 536, 578, 883, 1057, 1262
AluI	AGCT	7 cut(s)	289, 352, 536, 578, 883, 1057, 1262
Alw26I	GTCTC	1 cut(s)	187
AlwNI	CAGNNNCTG	1 cut(s)	352
Aor51HI	AGCGCT	1 cut(s)	361
AoxI	GGCC	2 cut(s)	103, 618
ApeKI	GCWGC	3 cut(s)	349, 421, 892
ApoI	RAATTY	2 cut(s)	1115, 1226
Asp700I	GAANNNNTTC	1 cut(s)	141
AspLEI	GCGC	3 cut(s)	362, 472, 718
AsuHPI	GGTGA	1 cut(s)	1304
AsuII	TTCGAA	1 cut(s)	707
BaeI	ACNNNNGTAYC	2 cut(s)	644, 677
BalI	TGGCCA	1 cut(s)	105
BbvI	GCAGC	3 cut(s)	361, 408, 904
BccI	CCATC	3 cut(s)	488, 560, 1094
BceAI	ACGGC	2 cut(s)	616, 737
BcgI	CGANNNNNNTGC	2 cut(s)	199, 233
BciT130I	CCWGG	2 cut(s)	793, 851
BciVI	GTATCC	1 cut(s)	1060
BclI	TGATCA	3 cut(s)	163, 354, 868
BcoDI	GTCTC	1 cut(s)	187
BfaI	CTAG	3 cut(s)	753, 906, 912
BfoI	RGCGCY	1 cut(s)	363
BfuI	GTATCC	1 cut(s)	1060
BisI	GCNGC	4 cut(s)	283, 350, 422, 893
BlsI	GCNGC	4 cut(s)	284, 351, 423, 894
BmcAI	AGTACT	1 cut(s)	341
Bme1390I	CCNGG	2 cut(s)	793, 851
BmeRI	GACNNNNNGTC	1 cut(s)	586
BmrFI	CCNGG	2 cut(s)	793, 851
BmsI	GCATC	1 cut(s)	15
BpmI	CTGGAG	1 cut(s)	1079
Bpu14I	TTCGAA	1 cut(s)	707
BpuEI	CTTGAG	2 cut(s)	302, 1250
BsaAI	YACGTR	1 cut(s)	610
BsaJI	CCNNGG	2 cut(s)	791, 850
Bsc4I	CCNNNNNNNGG	2 cut(s)	112, 638
Bse118I	RCCGGY	1 cut(s)	620
Bse1I	ACTGG	1 cut(s)	106
Bse3DI	GCAATG	1 cut(s)	1065
BseBI	CCWGG	2 cut(s)	793, 851
BseDI	CCNNGG	2 cut(s)	791, 850
BseGI	GGATG	1 cut(s)	1276
BseLI	CCNNNNNNNGG	2 cut(s)	112, 638
BseMI	GCAATG	1 cut(s)	1065
BseMII	CTCAG	3 cut(s)	402, 870, 1155
BseNI	ACTGG	1 cut(s)	106
BseXI	GCAGC	3 cut(s)	361, 408, 904
Bsh1236I	CGCG	2 cut(s)	399, 718
BshFI	GGCC	2 cut(s)	105, 620
BsiSI	CCGG	1 cut(s)	621
BslFI	GGGAC	1 cut(s)	999
BslI	CCNNNNNNNGG	2 cut(s)	112, 638
BsmAI	GTCTC	1 cut(s)	187
BsmFI	GGGAC	1 cut(s)	999
BsmI	GAATGC	1 cut(s)	695
BsnI	GGCC	2 cut(s)	105, 620
Bsp119I	TTCGAA	1 cut(s)	707
Bsp143I	GATC	3 cut(s)	163, 354, 868
BspACI	CCGC	3 cut(s)	282, 397, 476
BspANI	GGCC	2 cut(s)	105, 620
BspCNI	CTCAG	3 cut(s)	403, 871, 1156
BspFNI	CGCG	2 cut(s)	399, 718
BspQI	GCTCTTC	1 cut(s)	670
BspT104I	TTCGAA	1 cut(s)	707
BsrDI	GCAATG	1 cut(s)	1065
BsrFI	RCCGGY	1 cut(s)	620
BsrI	ACTGG	1 cut(s)	106
BssAI	RCCGGY	1 cut(s)	620
BssECI	CCNNGG	2 cut(s)	791, 850
BssMI	GATC	3 cut(s)	163, 354, 868
Bst2UI	CCWGG	2 cut(s)	793, 851
Bst4CI	ACNGT	1 cut(s)	1292
Bst6I	CTCTTC	1 cut(s)	670
BstBAI	YACGTR	1 cut(s)	610
BstBI	TTCGAA	1 cut(s)	707
BstC8I	GCNNGC	3 cut(s)	287, 474, 885
BstDEI	CTNAG	4 cut(s)	411, 696, 879, 1164
BstF5I	GGATG	1 cut(s)	1276
BstFNI	CGCG	2 cut(s)	399, 718
BstH2I	RGCGCY	1 cut(s)	363
BstHHI	GCGC	3 cut(s)	362, 472, 718
BstKTI	GATC	3 cut(s)	166, 357, 871
BstMAI	GTCTC	1 cut(s)	187
BstMBI	GATC	3 cut(s)	163, 354, 868
BstMWI	GCNNNNNNNGC	4 cut(s)	533, 889, 1160, 1294
BstNI	CCWGG	2 cut(s)	793, 851
BstSCI	CCNGG	2 cut(s)	791, 849
BstUI	CGCG	2 cut(s)	399, 718
BstV1I	GCAGC	3 cut(s)	361, 408, 904
BsuI	GTATCC	1 cut(s)	1060
BsuRI	GGCC	2 cut(s)	105, 620
BtsCI	GGATG	1 cut(s)	1276
Cac8I	GCNNGC	3 cut(s)	287, 474, 885
CaiI	CAGNNNCTG	1 cut(s)	352
CfoI	GCGC	3 cut(s)	362, 472, 718
Cfr10I	RCCGGY	1 cut(s)	620
Csp6I	GTAC	2 cut(s)	340, 607
CviAII	CATG	4 cut(s)	83, 235, 638, 1284
CviQI	GTAC	2 cut(s)	340, 607
DdeI	CTNAG	4 cut(s)	411, 696, 879, 1164
DpnI	GATC	3 cut(s)	165, 356, 870
DpnII	GATC	3 cut(s)	163, 354, 868
DraI	TTTAAA	1 cut(s)	927
DraIII	CACNNNGTG	1 cut(s)	301
DriI	GACNNNNNGTC	1 cut(s)	586
EaeI	YGGCCR	2 cut(s)	103, 618
Eam1104I	CTCTTC	1 cut(s)	670
Eam1105I	GACNNNNNGTC	1 cut(s)	586
EarI	CTCTTC	1 cut(s)	670
Eco32I	GATATC	1 cut(s)	940
Eco47III	AGCGCT	1 cut(s)	361
Eco57I	CTGAAG	2 cut(s)	464, 561
EcoRII	CCWGG	2 cut(s)	791, 849
EcoRV	GATATC	1 cut(s)	940
EcoT22I	ATGCAT	1 cut(s)	1332
FaeI	CATG	4 cut(s)	86, 238, 641, 1287
FalI	AAGNNNNNCTT	2 cut(s)	483, 515
FaqI	GGGAC	1 cut(s)	999
FatI	CATG	4 cut(s)	82, 234, 637, 1283
FauNDI	CATATG	2 cut(s)	562, 1326
FbaI	TGATCA	3 cut(s)	163, 354, 868
Fnu4HI	GCNGC	4 cut(s)	283, 350, 422, 893
FokI	GGATG	1 cut(s)	1283
Fsp4HI	GCNGC	4 cut(s)	283, 350, 422, 893
FspBI	CTAG	3 cut(s)	753, 906, 912
GlaI	GCGC	3 cut(s)	361, 471, 717
GluI	GCNGC	4 cut(s)	283, 350, 422, 893
GsuI	CTGGAG	1 cut(s)	1079
HaeII	RGCGCY	1 cut(s)	363
HaeIII	GGCC	2 cut(s)	105, 620
HapII	CCGG	1 cut(s)	621
HhaI	GCGC	3 cut(s)	362, 472, 718
Hin1II	CATG	4 cut(s)	86, 238, 641, 1287
Hin6I	GCGC	3 cut(s)	360, 470, 716
HinP1I	GCGC	3 cut(s)	360, 470, 716
HinfI	GANTC	2 cut(s)	195, 479
HpaII	CCGG	1 cut(s)	621
HphI	GGTGA	1 cut(s)	1304
Hpy166II	GTNNAC	4 cut(s)	391, 1020, 1179, 1281
Hpy188I	TCNGA	7 cut(s)	147, 454, 651, 845, 862, 873, 1129
Hpy188III	TCNNGA	3 cut(s)	539, 1093, 1120
Hpy8I	GTNNAC	4 cut(s)	391, 1020, 1179, 1281
Hpy99I	CGWCG	1 cut(s)	723
HpyAV	CCTTC	4 cut(s)	439, 1131, 1135, 1228
HpyCH4III	ACNGT	1 cut(s)	1292
HpyCH4IV	ACGT	2 cut(s)	609, 864
HpyCH4V	TGCA	9 cut(s)	28, 86, 220, 505, 784, 827, 1030, 1148, 1330
HpyF10VI	GCNNNNNNNGC	4 cut(s)	533, 889, 1160, 1294
HpyF3I	CTNAG	4 cut(s)	411, 696, 879, 1164
HpySE526I	ACGT	2 cut(s)	609, 864
Hsp92II	CATG	4 cut(s)	86, 238, 641, 1287
HspAI	GCGC	3 cut(s)	360, 470, 716
Ksp22I	TGATCA	3 cut(s)	163, 354, 868
Kzo9I	GATC	3 cut(s)	163, 354, 868
LguI	GCTCTTC	1 cut(s)	670
LmnI	GCTCC	1 cut(s)	541
Lsp1109I	GCAGC	3 cut(s)	361, 408, 904
LweI	GCATC	1 cut(s)	15
MaeI	CTAG	3 cut(s)	753, 906, 912
MaeII	ACGT	2 cut(s)	609, 864
MaeIII	GTNAC	1 cut(s)	1107
MalI	GATC	3 cut(s)	165, 356, 870
MboI	GATC	3 cut(s)	163, 354, 868
MboII	GAAGA	4 cut(s)	687, 1213, 1256, 1329
MfeI	CAATTG	2 cut(s)	822, 1044
MlsI	TGGCCA	1 cut(s)	105
MluCI	AATT	7 cut(s)	126, 159, 669, 822, 1044, 1115, 1226
MluNI	TGGCCA	1 cut(s)	105
MlyI	GAGTC	1 cut(s)	189
MmeI	TCCRAC	5 cut(s)	133, 432, 507, 823, 1221
MnlI	CCTC	9 cut(s)	104, 223, 306, 316, 377, 749, 1048, 1097, 1153
Mox20I	TGGCCA	1 cut(s)	105
Mph1103I	ATGCAT	1 cut(s)	1332
MroXI	GAANNNNTTC	1 cut(s)	141
MscI	TGGCCA	1 cut(s)	105
MseI	TTAA	5 cut(s)	120, 129, 201, 926, 1196
MslI	CAYNNNNRTG	1 cut(s)	1025
Msp20I	TGGCCA	1 cut(s)	105
MspA1I	CMGCKG	1 cut(s)	352
MspI	CCGG	1 cut(s)	621
MspR9I	CCNGG	2 cut(s)	793, 851
MunI	CAATTG	2 cut(s)	822, 1044
Mva1269I	GAATGC	1 cut(s)	695
MvaI	CCWGG	2 cut(s)	793, 851
MvnI	CGCG	2 cut(s)	399, 718
MwoI	GCNNNNNNNGC	4 cut(s)	533, 889, 1160, 1294
NdeI	CATATG	2 cut(s)	562, 1326
NdeII	GATC	3 cut(s)	163, 354, 868
NlaIII	CATG	4 cut(s)	86, 238, 641, 1287
NsiI	ATGCAT	1 cut(s)	1332
NspV	TTCGAA	1 cut(s)	707
OliI	CACNNNNGTG	1 cut(s)	1025
PciSI	GCTCTTC	1 cut(s)	670
PctI	GAATGC	1 cut(s)	695
PdmI	GAANNNNTTC	1 cut(s)	141
PfeI	GAWTC	1 cut(s)	479
PflMI	CCANNNNNTGG	1 cut(s)	112
PkrI	GCNGC	4 cut(s)	284, 351, 423, 894
PleI	GAGTC	1 cut(s)	189
PpsI	GAGTC	1 cut(s)	189
Ppu21I	YACGTR	1 cut(s)	610
Psp6I	CCWGG	2 cut(s)	791, 849
PspGI	CCWGG	2 cut(s)	791, 849
PstNI	CAGNNNCTG	1 cut(s)	352
PvuII	CAGCTG	1 cut(s)	352
RsaI	GTAC	2 cut(s)	341, 608
RsaNI	GTAC	2 cut(s)	340, 607
RseI	CAYNNNNRTG	1 cut(s)	1025
SapI	GCTCTTC	1 cut(s)	670
SaqAI	TTAA	5 cut(s)	120, 129, 201, 926, 1196
SatI	GCNGC	4 cut(s)	283, 350, 422, 893
Sau3AI	GATC	3 cut(s)	163, 354, 868
ScaI	AGTACT	1 cut(s)	341
SchI	GAGTC	1 cut(s)	189
ScrFI	CCNGG	2 cut(s)	793, 851
SfaNI	GCATC	1 cut(s)	15
SfuI	TTCGAA	1 cut(s)	707
SmiI	ATTTAAAT	1 cut(s)	927
SmiMI	CAYNNNNRTG	1 cut(s)	1025
SmlI	CTYRAG	2 cut(s)	317, 1265
SmoI	CTYRAG	2 cut(s)	317, 1265
Sse9I	AATT	7 cut(s)	126, 159, 669, 822, 1044, 1115, 1226
SsiI	CCGC	3 cut(s)	282, 397, 476
SspMI	CTAG	3 cut(s)	753, 906, 912
StyD4I	CCNGG	2 cut(s)	791, 849
SwaI	ATTTAAAT	1 cut(s)	927
TaaI	ACNGT	1 cut(s)	1292
TaiI	ACGT	2 cut(s)	612, 867
TaqI	TCGA	3 cut(s)	305, 707, 1119
TasI	AATT	7 cut(s)	126, 159, 669, 822, 1044, 1115, 1226
TatI	WGTACW	1 cut(s)	339
TauI	GCSGC	1 cut(s)	285
TfiI	GAWTC	1 cut(s)	479
Tru1I	TTAA	5 cut(s)	120, 129, 201, 926, 1196
Tru9I	TTAA	5 cut(s)	120, 129, 201, 926, 1196
TseI	GCWGC	3 cut(s)	349, 421, 892
TspDTI	ATGAA	6 cut(s)	626, 726, 757, 1173, 1181, 1214
TspGWI	ACGGA	1 cut(s)	1149
Van91I	CCANNNNNTGG	1 cut(s)	112
XapI	RAATTY	2 cut(s)	1115, 1226
XmnI	GAANNNNTTC	1 cut(s)	141
XspI	CTAG	3 cut(s)	753, 906, 912
ZrmI	AGTACT	1 cut(s)	341
Zsp2I	ATGCAT	1 cut(s)	1332

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.