MD02G1203200.v1.1

microtubule binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001938
Physical Location & Seq
Forward (+)
20240195 .. 20245408
5214 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1203200.v1.1.491

Sequence Viewer

Length: 2100 bp
ATGGAAACAAAGTTTGAATCTTTGCTGCAGGAACTGCAGAAAATATGGGATGAAGTTGGAGTGCCTGATGTCGAAAGGGATGCAATGTTTCTAAAAATCGAACAAGAGTGTATGGAGGTATCTAGGAGAAAAGTTGACGAGGCAAAAAAATGTAAAGCTCAGCTTCTGCAAAAAATTGCTAATCACAAAGTAGAGCTTGTAGACATATGTGCTGCATTGGGAGAGCAGCCACAACAACTTGATCAAAGGGCCAGTGGAAGTTTGAAGAAAGAGCTTGAAACCATTATCCAACAACTAGAGGACATGAAGATGCGGAAAATGGAAAGAAGGAATGAATTCTTTGTTGTCTTAGATCAATTACAGAAAATTTCGAATGCAATAGGCAAATGCATGAAAGACAGTCTGTACAAAAAGGTAGTGGAAGAGACTGACTTGTCCTTGAAGAGACTTGAAGAATTGCGTAGACGGCTGCTTGAATATCAAGATGAGAAGAGAAACCGTTTGAATCTGGTAATGGACCACCTAAACATAATAAGCTCACTCAGCTTGGTTCTCGGTATGGATTTTAAGCAAACAATTCATGAAATCCACCCCACTTTGATTGATTCTGAGGGAGCAAAGGACATAAGCAACAATACAATTGATAGCTTGGGTAACTCGATACAAAGGTTGAGAAAGCTCAAGAATCAGAGATGGCAGAGGCTCCAAGATCATGCAAGTGCTCTGTTGGAACTGTGGAATCTGATGGATACACCAATAGAGGAACAAAATAAATTTCAGAATGTAACTAGTCATATAACTGCTTTAGAATCTGAAATCACTGGGCCTAACATGCTGTCTACAGACGTTCTAAATCATGTTGAGGCAGAAGTGTCAAGGTTGCAGCAGCTCAAATCAAGTAAGCTAAAAGAGATTAGTCAGAAAAAGAAGTTGGAACTGGAGGAGATATGCAAGCGGTCTCATATGGTTGCAGAAACATTTAGTGCAATGGAACACTCAATTGAAGCAGCAGAGTCTGGAACTGCGGACACTGCATGCCTGTTGGAACAGATTGATCTCCAAATCACAAGGGCAAAAGAGGAAGCTTCAAGCAGGAAAGAAATACTAGAAAAGGTTGAGAAGTGGTTGGCTGCGCGTCAAGAAGAATGTTGGCTGGAGGAGTACAACAGGGATGAAAACCGCTATAATGCAGGGAGAGGAGCCCATCTTACATTGAAACGTGCAGAGAAAGCTCGTGCTTTGGTAAACAAAATTCCAGGGATGTTGGAAGCATTGACTTCAAAAGCCACAGCTTGGGAAAGAGAAAGAGGAACTGAATTCTTGTATGATGGTGGCAAACTTCTTTCAATGCTCAATCAGTACAGCACCTTAAGGCAGGAGAAAGAGCAAGAAAAGCAGAGACAGAGAGATATAAAAAAATTTCAGGGGCTGGTGCTAGTTGAGCAGGAAGCATTTTATGGGTCAAAACGTAGTCCATCCAAGAGTGGAAAGAAGGCTTCCAATAGAAAAATGTCCCTAGGTGGAGCATTAATCCGAAGCCAAAAGTATGAAAAAGTTACTCCGCCTTTACACCCTACAAAGAAGGGTGACTTTCTCCATCGAAGTAGCTCTCGCCAGCAATACAGTGAAGTGGAAGCTCCATCCTTTGGAAGGAGAACCTCAGAGATCTATGGTCATTCAGTGAAGAAGCGCCCAACGAGTGCTGCAAAGGCTCTTGAAATGGAATCCCCATTGATCCGGAAACCGCTCTCTCCTGTCTATTCTGAAGTGTCATCTATGGCCAACATTGCAAATTACTTTGAAGGTCAGAAGAAACAACAGGAGACAAACATCCAAGCAAGCCATCTTTTTAGTGAAAAGCCAATCATGACTCCTTCCAAGCCGAAAAACGTGGGTGATGATGCAGAGAATAGAACCCCAAAGACGAAGCCAATTCCAGTACCTCCAACTCCTTCAACAATATCAGTTCATATGCTGACGGCCATGACACCAGCCACACCTTTCACTTCTGGTGATTACAAGATTGAGAAAGCTGATCAACGACTTGAGTACTCGTTCGAGGAGGTGAGAGCAATTAGATATGAACTTTGTAGCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

700

Amino Acids

80.19

Weight (kDa)

8.73

Isoelectric Point (pI)

56.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 7 - 495 1.1e-97 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 433
AccB7I CCANNNNNTGG 2 cut(s) 1293, 1646
AccBSI CCGCTC 1 cut(s) 1747
AccI GTMKAC 3 cut(s) 201, 463, 839
AccII CGCG 1 cut(s) 1135
AccIII TCCGGA 1 cut(s) 1737
AciI CCGC 6 cut(s) 313, 955, 1025, 1180, 1562, 1745
AclWI GGATC 1 cut(s) 1729
AcoI YGGCCR 2 cut(s) 1779, 1980
AcsI RAATTY 6 cut(s) 335, 366, 773, 1251, 1316, 1418
AcuI CTGAAG 1 cut(s) 1785
AfaI GTAC 5 cut(s) 407, 1163, 1361, 1941, 2051
AfiI CCNNNNNNNGG 3 cut(s) 1293, 1646, 1650
AflII CTTAAG 1 cut(s) 1369
AhlI ACTAGT 1 cut(s) 788
AjnI CCWGG 1 cut(s) 1255
AjuI GAANNNNNNNTTGG 2 cut(s) 914, 946
Alw21I GWGCWC 1 cut(s) 724
Alw26I GTCTC 5 cut(s) 419, 439, 963, 1393, 1817
AlwI GGATC 1 cut(s) 1729
AlwNI CAGNNNCTG 4 cut(s) 34, 166, 1016, 1429
Aor13HI TCCGGA 1 cut(s) 1737
AoxI GGCC 4 cut(s) 249, 824, 1779, 1980
ApeKI GCWGC 9 cut(s) 25, 212, 226, 469, 883, 886, 1007, 1130, 1703
ApoI RAATTY 6 cut(s) 335, 366, 773, 1251, 1316, 1418
AseI ATTAAT 1 cut(s) 1529
Asp700I GAANNNNTTC 1 cut(s) 335
AspA2I CCTAGG 1 cut(s) 1516
AspLEI GCGC 2 cut(s) 1135, 1692
AspS9I GGNCC 3 cut(s) 249, 517, 824
AsuHPI GGTGA 4 cut(s) 1598, 1907, 2024, 2077
AsuII TTCGAA 1 cut(s) 371
AvaII GGWCC 1 cut(s) 517
AvrII CCTAGG 1 cut(s) 1516
BaeI ACNNNNGTAYC 2 cut(s) 653, 686
BalI TGGCCA 1 cut(s) 1781
BanII GRGCYC 1 cut(s) 1204
BauI CACGAG 1 cut(s) 1233
Bbv12I GWGCWC 1 cut(s) 724
BbvI GCAGC 9 cut(s) 12, 199, 238, 456, 895, 898, 1019, 1117, 1690
BccI CCATC 8 cut(s) 687, 739, 1212, 1322, 1483, 1605, 1648, 1851
BceAI ACGGC 2 cut(s) 482, 1995
BcgI CGANNNNNNTGC 2 cut(s) 62, 96
BciT130I CCWGG 1 cut(s) 1257
BciVI GTATCC 1 cut(s) 742
BclI TGATCA 2 cut(s) 241, 2035
BcoDI GTCTC 5 cut(s) 419, 439, 963, 1393, 1817
BcuI ACTAGT 1 cut(s) 788
BfaI CTAG 6 cut(s) 123, 296, 789, 1106, 1436, 1517
BfmI CTRYAG 3 cut(s) 26, 35, 840
BfoI RGCGCY 1 cut(s) 1693
BfrI CTTAAG 1 cut(s) 1369
BfuI GTATCC 1 cut(s) 742
BglII AGATCT 1 cut(s) 1665
BisI GCNGC 9 cut(s) 26, 213, 227, 470, 884, 887, 1008, 1131, 1704
BlnI CCTAGG 1 cut(s) 1516
BlpI GCTNAGC 1 cut(s) 159
BlsI GCNGC 9 cut(s) 27, 214, 228, 471, 885, 888, 1009, 1132, 1705
BmcAI AGTACT 1 cut(s) 2051
Bme1390I CCNGG 1 cut(s) 1257
Bme18I GGWCC 1 cut(s) 517
BmgT120I GGNCC 3 cut(s) 249, 517, 824
BmiI GGNNCC 2 cut(s) 704, 1201
BmrFI CCNGG 1 cut(s) 1257
BmrI ACTGGG 1 cut(s) 831
BmsI GCATC 3 cut(s) 70, 300, 1891
BmuI ACTGGG 1 cut(s) 831
BpmI CTGGAG 2 cut(s) 959, 1175
Bpu1102I GCTNAGC 1 cut(s) 159
Bpu14I TTCGAA 1 cut(s) 371
BpuEI CTTGAG 2 cut(s) 665, 2066
BsaI GGTCTC 1 cut(s) 963
BsaJI CCNNGG 2 cut(s) 1256, 1516
BsaWI WCCGGW 1 cut(s) 1737
Bsc4I CCNNNNNNNGG 3 cut(s) 1293, 1646, 1650
Bse1I ACTGG 4 cut(s) 252, 826, 942, 1937
Bse3DI GCAATG 3 cut(s) 90, 993, 1785
BseAI TCCGGA 1 cut(s) 1737
BseBI CCWGG 1 cut(s) 1257
BseDI CCNNGG 2 cut(s) 1256, 1516
BseGI GGATG 7 cut(s) 55, 85, 1177, 1266, 1475, 1640, 1830
BseLI CCNNNNNNNGG 3 cut(s) 1293, 1646, 1650
BseMI GCAATG 3 cut(s) 90, 993, 1785
BseMII CTCAG 4 cut(s) 173, 556, 600, 1674
BseNI ACTGG 4 cut(s) 252, 826, 942, 1937
BseRI GAGGAG 4 cut(s) 956, 1172, 1212, 2075
BseXI GCAGC 9 cut(s) 12, 199, 238, 456, 895, 898, 1019, 1117, 1690
BsgI GTGCAG 1 cut(s) 1242
Bsh1236I CGCG 1 cut(s) 1135
BshFI GGCC 4 cut(s) 251, 826, 1781, 1982
BsiHKAI GWGCWC 1 cut(s) 724
BsiSI CCGG 1 cut(s) 1738
BslFI GGGAC 1 cut(s) 1498
BslI CCNNNNNNNGG 3 cut(s) 1293, 1646, 1650
BsmAI GTCTC 5 cut(s) 419, 439, 963, 1393, 1817
BsmFI GGGAC 1 cut(s) 1498
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 4 cut(s) 251, 826, 1781, 1982
Bso31I GGTCTC 1 cut(s) 963
Bsp119I TTCGAA 1 cut(s) 371
Bsp1286I GDGCHC 2 cut(s) 724, 1204
Bsp13I TCCGGA 1 cut(s) 1737
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 7 cut(s) 241, 352, 709, 1054, 1665, 1734, 2035
Bsp1720I GCTNAGC 1 cut(s) 159
BspACI CCGC 6 cut(s) 313, 955, 1025, 1180, 1562, 1745
BspANI GGCC 4 cut(s) 251, 826, 1781, 1982
BspCNI CTCAG 4 cut(s) 172, 555, 601, 1673
BspEI TCCGGA 1 cut(s) 1737
BspFNI CGCG 1 cut(s) 1135
BspHI TCATGA 2 cut(s) 580, 1866
BspLI GGNNCC 2 cut(s) 704, 1201
BspMAI CTGCAG 2 cut(s) 30, 39
BspPI GGATC 1 cut(s) 1729
BspT104I TTCGAA 1 cut(s) 371
BspTI CTTAAG 1 cut(s) 1369
BspTNI GGTCTC 1 cut(s) 963
BsrBI CCGCTC 1 cut(s) 1747
BsrDI GCAATG 3 cut(s) 90, 993, 1785
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 4 cut(s) 252, 826, 942, 1937
BssECI CCNNGG 2 cut(s) 1256, 1516
BssMI GATC 7 cut(s) 241, 352, 709, 1054, 1665, 1734, 2035
BssSI CACGAG 1 cut(s) 1233
BssT1I CCWWGG 1 cut(s) 1516
Bst2BI CACGAG 1 cut(s) 1233
Bst2UI CCWGG 1 cut(s) 1257
Bst4CI ACNGT 4 cut(s) 401, 500, 735, 1625
Bst6I CTCTTC 3 cut(s) 417, 437, 485
BstAFI CTTAAG 1 cut(s) 1369
BstAPI GCANNNNNTGC 1 cut(s) 34
BstAUI TGTACA 1 cut(s) 405
BstBI TTCGAA 1 cut(s) 371
BstC8I GCNNGC 4 cut(s) 953, 1036, 1616, 1840
BstDEI CTNAG 5 cut(s) 159, 349, 542, 609, 1660
BstENI CCTNNNNNAGG 1 cut(s) 1648
BstF5I GGATG 7 cut(s) 55, 85, 1177, 1266, 1475, 1640, 1830
BstFNI CGCG 1 cut(s) 1135
BstH2I RGCGCY 1 cut(s) 1693
BstHHI GCGC 2 cut(s) 1135, 1692
BstKTI GATC 7 cut(s) 244, 355, 712, 1057, 1668, 1737, 2038
BstMAI GTCTC 5 cut(s) 419, 439, 963, 1393, 1817
BstMBI GATC 7 cut(s) 241, 352, 709, 1054, 1665, 1734, 2035
BstNI CCWGG 1 cut(s) 1257
BstNSI RCATGY 2 cut(s) 835, 1038
BstSCI CCNGG 1 cut(s) 1255
BstSFI CTRYAG 3 cut(s) 26, 35, 840
BstUI CGCG 1 cut(s) 1135
BstV1I GCAGC 9 cut(s) 12, 199, 238, 456, 895, 898, 1019, 1117, 1690
BstX2I RGATCY 1 cut(s) 1665
BstYI RGATCY 1 cut(s) 1665
BsuI GTATCC 1 cut(s) 742
BsuRI GGCC 4 cut(s) 251, 826, 1781, 1982
BtsCI GGATG 7 cut(s) 55, 85, 1177, 1266, 1475, 1640, 1830
BtsI GCAGTG 1 cut(s) 1029
BtsIMutI CAGTG 5 cut(s) 259, 819, 1029, 1630, 1686
Cac8I GCNNGC 4 cut(s) 953, 1036, 1616, 1840
CaiI CAGNNNCTG 4 cut(s) 34, 166, 1016, 1429
CciI TCATGA 2 cut(s) 580, 1866
CfoI GCGC 2 cut(s) 1135, 1692
Cfr13I GGNCC 3 cut(s) 249, 517, 824
CseI GACGC 1 cut(s) 1124
Csp6I GTAC 5 cut(s) 406, 1162, 1360, 1940, 2050
CspCI CAANNNNNGTGG 2 cut(s) 583, 618
CviAII CATG 9 cut(s) 304, 391, 581, 713, 832, 857, 1035, 1867, 1984
CviQI GTAC 5 cut(s) 406, 1162, 1360, 1940, 2050
DdeI CTNAG 5 cut(s) 159, 349, 542, 609, 1660
DpnI GATC 7 cut(s) 243, 354, 711, 1056, 1667, 1736, 2037
DpnII GATC 7 cut(s) 241, 352, 709, 1054, 1665, 1734, 2035
DrdI GACNNNNNNGTC 1 cut(s) 433
DseDI GACNNNNNNGTC 1 cut(s) 433
EaeI YGGCCR 2 cut(s) 1779, 1980
Eam1104I CTCTTC 3 cut(s) 417, 437, 485
EarI CTCTTC 3 cut(s) 417, 437, 485
EciI GGCGGA 1 cut(s) 1551
Eco130I CCWWGG 1 cut(s) 1516
Eco24I GRGCYC 1 cut(s) 1204
Eco31I GGTCTC 1 cut(s) 963
Eco47I GGWCC 1 cut(s) 517
Eco57I CTGAAG 1 cut(s) 1785
EcoNI CCTNNNNNAGG 1 cut(s) 1648
EcoRI GAATTC 2 cut(s) 335, 1316
EcoRII CCWGG 1 cut(s) 1255
EcoT14I CCWWGG 1 cut(s) 1516
EcoT22I ATGCAT 1 cut(s) 392
EcoT38I GRGCYC 1 cut(s) 1204
ErhI CCWWGG 1 cut(s) 1516
FaeI CATG 9 cut(s) 307, 394, 584, 716, 835, 860, 1038, 1870, 1987
FalI AAGNNNNNCTT 6 cut(s) 147, 179, 180, 212, 1574, 1606
FaqI GGGAC 1 cut(s) 1498
FatI CATG 9 cut(s) 303, 390, 580, 712, 831, 856, 1034, 1866, 1983
FauNDI CATATG 3 cut(s) 206, 963, 1971
FbaI TGATCA 2 cut(s) 241, 2035
FblI GTMKAC 3 cut(s) 201, 463, 839
Fnu4HI GCNGC 9 cut(s) 26, 213, 227, 470, 884, 887, 1008, 1131, 1704
FokI GGATG 7 cut(s) 62, 92, 1184, 1273, 1462, 1627, 1817
FriOI GRGCYC 1 cut(s) 1204
Fsp4HI GCNGC 9 cut(s) 26, 213, 227, 470, 884, 887, 1008, 1131, 1704
FspBI CTAG 6 cut(s) 123, 296, 789, 1106, 1436, 1517
GlaI GCGC 2 cut(s) 1134, 1691
GluI GCNGC 9 cut(s) 26, 213, 227, 470, 884, 887, 1008, 1131, 1704
GsuI CTGGAG 2 cut(s) 959, 1175
HaeII RGCGCY 1 cut(s) 1693
HaeIII GGCC 4 cut(s) 251, 826, 1781, 1982
HapII CCGG 1 cut(s) 1738
HgaI GACGC 1 cut(s) 1124
HhaI GCGC 2 cut(s) 1135, 1692
Hin1II CATG 9 cut(s) 307, 394, 584, 716, 835, 860, 1038, 1870, 1987
Hin6I GCGC 2 cut(s) 1133, 1690
HinP1I GCGC 2 cut(s) 1133, 1690
HincII GTYRAC 1 cut(s) 136
HindII GTYRAC 1 cut(s) 136
HindIII AAGCTT 1 cut(s) 1083
HinfI GANTC 9 cut(s) 17, 505, 605, 685, 739, 809, 1013, 1724, 1870
HpaII CCGG 1 cut(s) 1738
HphI GGTGA 4 cut(s) 1598, 1907, 2024, 2077
Hpy166II GTNNAC 5 cut(s) 136, 202, 464, 840, 1246
Hpy188III TCNNGA 8 cut(s) 482, 581, 682, 1017, 1139, 1715, 1738, 1867
Hpy8I GTNNAC 5 cut(s) 136, 202, 464, 840, 1246
HpyAV CCTTC 7 cut(s) 321, 1486, 1576, 1644, 1796, 1884, 1962
HpyCH4III ACNGT 4 cut(s) 401, 500, 735, 1625
HpyCH4IV ACGT 4 cut(s) 846, 1219, 1468, 1890
HpyF3I CTNAG 5 cut(s) 159, 349, 542, 609, 1660
HpySE526I ACGT 4 cut(s) 846, 1219, 1468, 1890
Hsp92II CATG 9 cut(s) 307, 394, 584, 716, 835, 860, 1038, 1870, 1987
HspAI GCGC 2 cut(s) 1133, 1690
Kpn2I TCCGGA 1 cut(s) 1737
Ksp22I TGATCA 2 cut(s) 241, 2035
Kzo9I GATC 7 cut(s) 241, 352, 709, 1054, 1665, 1734, 2035
LmnI GCTCC 5 cut(s) 614, 708, 1199, 1523, 1642
Lsp1109I GCAGC 9 cut(s) 12, 199, 238, 456, 895, 898, 1019, 1117, 1690
LweI GCATC 3 cut(s) 70, 300, 1891
MaeI CTAG 6 cut(s) 123, 296, 789, 1106, 1436, 1517
MaeII ACGT 4 cut(s) 846, 1219, 1468, 1890
MaeIII GTNAC 4 cut(s) 653, 784, 1555, 1586
MalI GATC 7 cut(s) 243, 354, 711, 1056, 1667, 1736, 2037
MbiI CCGCTC 1 cut(s) 1747
MboI GATC 7 cut(s) 241, 352, 709, 1054, 1665, 1734, 2035
MboII GAAGA 9 cut(s) 277, 319, 434, 454, 464, 502, 1154, 1696, 1822
MfeI CAATTG 2 cut(s) 639, 999
MflI RGATCY 1 cut(s) 1665
MhlI GDGCHC 2 cut(s) 724, 1204
MlsI TGGCCA 1 cut(s) 1781
MluNI TGGCCA 1 cut(s) 1781
MlyI GAGTC 2 cut(s) 1022, 1864
MmeI TCCRAC 7 cut(s) 37, 313, 708, 912, 1023, 1245, 1970
Mox20I TGGCCA 1 cut(s) 1781
Mph1103I ATGCAT 1 cut(s) 392
MroI TCCGGA 1 cut(s) 1737
MroXI GAANNNNTTC 1 cut(s) 335
MscI TGGCCA 1 cut(s) 1781
MseI TTAA 3 cut(s) 567, 1370, 1529
MslI CAYNNNNRTG 2 cut(s) 308, 717
Msp20I TGGCCA 1 cut(s) 1781
MspCI CTTAAG 1 cut(s) 1369
MspI CCGG 1 cut(s) 1738
MspR9I CCNGG 1 cut(s) 1257
MunI CAATTG 2 cut(s) 639, 999
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 1 cut(s) 1257
MvnI CGCG 1 cut(s) 1135
NdeI CATATG 3 cut(s) 206, 963, 1971
NdeII GATC 7 cut(s) 241, 352, 709, 1054, 1665, 1734, 2035
NlaIII CATG 9 cut(s) 307, 394, 584, 716, 835, 860, 1038, 1870, 1987
NlaIV GGNNCC 2 cut(s) 704, 1201
NmuCI GTSAC 1 cut(s) 1586
NsiI ATGCAT 1 cut(s) 392
NspI RCATGY 2 cut(s) 835, 1038
NspV TTCGAA 1 cut(s) 371
PaeI GCATGC 1 cut(s) 1038
PagI TCATGA 2 cut(s) 580, 1866
PctI GAATGC 1 cut(s) 379
PdmI GAANNNNTTC 1 cut(s) 335
PfeI GAWTC 7 cut(s) 17, 505, 605, 685, 739, 809, 1724
PflMI CCANNNNNTGG 2 cut(s) 1293, 1646
PkrI GCNGC 9 cut(s) 27, 214, 228, 471, 885, 888, 1009, 1132, 1705
PleI GAGTC 2 cut(s) 1021, 1864
PpsI GAGTC 2 cut(s) 1021, 1864
PshBI ATTAAT 1 cut(s) 1529
Psp6I CCWGG 1 cut(s) 1255
PspGI CCWGG 1 cut(s) 1255
PspN4I GGNNCC 2 cut(s) 704, 1201
PspPI GGNCC 3 cut(s) 249, 517, 824
PstI CTGCAG 2 cut(s) 30, 39
PstNI CAGNNNCTG 4 cut(s) 34, 166, 1016, 1429
PsuI RGATCY 1 cut(s) 1665
RsaI GTAC 5 cut(s) 407, 1163, 1361, 1941, 2051
RsaNI GTAC 5 cut(s) 406, 1162, 1360, 1940, 2050
RseI CAYNNNNRTG 2 cut(s) 308, 717
SaqAI TTAA 3 cut(s) 567, 1370, 1529
SatI GCNGC 9 cut(s) 26, 213, 227, 470, 884, 887, 1008, 1131, 1704
Sau3AI GATC 7 cut(s) 241, 352, 709, 1054, 1665, 1734, 2035
Sau96I GGNCC 3 cut(s) 249, 517, 824
ScaI AGTACT 1 cut(s) 2051
SchI GAGTC 2 cut(s) 1022, 1864
ScrFI CCNGG 1 cut(s) 1257
SduI GDGCHC 2 cut(s) 724, 1204
SfaNI GCATC 3 cut(s) 70, 300, 1891
SfcI CTRYAG 3 cut(s) 26, 35, 840
SfuI TTCGAA 1 cut(s) 371
SinI GGWCC 1 cut(s) 517
SmiMI CAYNNNNRTG 2 cut(s) 308, 717
SmlI CTYRAG 3 cut(s) 680, 1369, 2045
SmoI CTYRAG 3 cut(s) 680, 1369, 2045
SpeI ACTAGT 1 cut(s) 788
SphI GCATGC 1 cut(s) 1038
SsiI CCGC 6 cut(s) 313, 955, 1025, 1180, 1562, 1745
SspMI CTAG 6 cut(s) 123, 296, 789, 1106, 1436, 1517
StyD4I CCNGG 1 cut(s) 1255
StyI CCWWGG 1 cut(s) 1516
TaaI ACNGT 4 cut(s) 401, 500, 735, 1625
TaiI ACGT 4 cut(s) 849, 1222, 1471, 1893
TaqI TCGA 6 cut(s) 72, 99, 371, 659, 1600, 2058
TatI WGTACW 4 cut(s) 405, 1161, 1359, 2049
TfiI GAWTC 7 cut(s) 17, 505, 605, 685, 739, 809, 1724
Tru1I TTAA 3 cut(s) 567, 1370, 1529
Tru9I TTAA 3 cut(s) 567, 1370, 1529
TscAI CASTG 5 cut(s) 259, 826, 1036, 1630, 1686
TseFI GTSAC 1 cut(s) 1586
TseI GCWGC 9 cut(s) 25, 212, 226, 469, 883, 886, 1007, 1130, 1703
Tsp45I GTSAC 1 cut(s) 1586
TspRI CASTG 5 cut(s) 259, 826, 1036, 1630, 1686
Van91I CCANNNNNTGG 2 cut(s) 1293, 1646
Vha464I CTTAAG 1 cut(s) 1369
VpaK11BI GGWCC 1 cut(s) 517
VspI ATTAAT 1 cut(s) 1529
XagI CCTNNNNNAGG 1 cut(s) 1648
XapI RAATTY 6 cut(s) 335, 366, 773, 1251, 1316, 1418
XceI RCATGY 2 cut(s) 835, 1038
XmaJI CCTAGG 1 cut(s) 1516
XmiI GTMKAC 3 cut(s) 201, 463, 839
XmnI GAANNNNTTC 1 cut(s) 335
XspI CTAG 6 cut(s) 123, 296, 789, 1106, 1436, 1517
ZrmI AGTACT 1 cut(s) 2051
Zsp2I ATGCAT 1 cut(s) 392
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.