Rh7BG451500

Microtubule associated protein (MAP65/ASE1 family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001938
Physical Location & Seq
Forward (+)
53024075 .. 53028206
4132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG451500.1

Sequence Viewer

Length: 624 bp
ATGAAGAAGCAGAAAATTGAAAGGAAGGACCAATTGTTTTCTGTCTTGGATCAGTTACAGAGACTTTCGACAGAAATAAGCAGGTCCTTGGAAGATAATCTGTACAAAATGGTGGTGGAAGAGACCGACTTGTCCTTGAAAAGGCTAGAAGAACTACATAGGCAGTTGCTTGAATATCAAGATGAGAAGAGGAACCGTTTGAAGCTGATAATGGACCACATGAGCATGCTAAACTCCCTGTGCTTGGTTCTTGGTATGGATTTTAAAAACACAATTCATGAAATCCACCCCACCTTAGATGATCTTAATGGAGAAAAAGACGTAACGAACAGCACAATCGAGGGTTTGGCTAATTCGGTTCAGATATTGAGAGAGGTCAAGATACAGAGATGGCAGAGGCTTCAAACTTTCGCAAGTGCCCTCTTGGAGATGTGGAATCTGATGGATACAACCATGGAGGAGCAAAAGAAATATCAAAATGTAACTAGTCGCATAGCTGCTTCAGAATCTGAAATCACTAAGCCTAACATTCTTTCTGTAGACCTTCTAAATGATGTTGAAGCTGAAGTGTCAAAGTTGGAGCAGCTCAAGTCAATTAAGCTCAAAGAGATCCTTCTGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

207

Amino Acids

24.27

Weight (kDa)

5.54

Isoelectric Point (pI)

47.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 1 - 205 6.8e-26 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 130
Acc36I ACCTGC 1 cut(s) 72
AccI GTMKAC 1 cut(s) 540
AclWI GGATC 2 cut(s) 57, 604
AcuI CTGAAG 2 cut(s) 486, 585
AfaI GTAC 1 cut(s) 104
AfiI CCNNNNNNNGG 2 cut(s) 141, 244
AgsI TTSAA 6 cut(s) 20, 139, 173, 202, 404, 560
AhlI ACTAGT 1 cut(s) 485
AluBI AGCT 5 cut(s) 205, 497, 563, 586, 601
AluI AGCT 5 cut(s) 205, 497, 563, 586, 601
Alw26I GTCTC 2 cut(s) 55, 116
AlwI GGATC 2 cut(s) 57, 604
AlwNI CAGNNNCTG 1 cut(s) 509
ApeKI GCWGC 2 cut(s) 497, 583
AspS9I GGNCC 3 cut(s) 28, 84, 214
AvaII GGWCC 3 cut(s) 28, 84, 214
BaeGI GKGCMC 1 cut(s) 421
BbvI GCAGC 2 cut(s) 484, 595
BccI CCATC 2 cut(s) 384, 436
BciVI GTATCC 1 cut(s) 439
BcoDI GTCTC 2 cut(s) 55, 116
BcuI ACTAGT 1 cut(s) 485
BfaI CTAG 2 cut(s) 146, 486
BfmI CTRYAG 1 cut(s) 537
BfuAI ACCTGC 1 cut(s) 72
BfuI GTATCC 1 cut(s) 439
BisI GCNGC 2 cut(s) 498, 584
BlsI GCNGC 2 cut(s) 499, 585
Bme18I GGWCC 3 cut(s) 28, 84, 214
BmgT120I GGNCC 3 cut(s) 28, 84, 214
BmiI GGNNCC 1 cut(s) 194
BpuEI CTTGAG 1 cut(s) 572
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 87, 453
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 244
BseDI CCNNGG 2 cut(s) 87, 453
BseLI CCNNNNNNNGG 2 cut(s) 141, 244
BseRI GAGGAG 1 cut(s) 473
BseSI GKGCMC 1 cut(s) 421
BseXI GCAGC 2 cut(s) 484, 595
BslI CCNNNNNNNGG 2 cut(s) 141, 244
BsmAI GTCTC 2 cut(s) 55, 116
Bso31I GGTCTC 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 421
Bsp1407I TGTACA 1 cut(s) 102
Bsp143I GATC 3 cut(s) 49, 301, 609
Bsp19I CCATGG 1 cut(s) 453
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 1 cut(s) 194
BspMI ACCTGC 1 cut(s) 72
BspPI GGATC 2 cut(s) 57, 604
BspTNI GGTCTC 1 cut(s) 116
BsrGI TGTACA 1 cut(s) 102
BssECI CCNNGG 2 cut(s) 87, 453
BssMI GATC 3 cut(s) 49, 301, 609
BssT1I CCWWGG 2 cut(s) 87, 453
Bst4CI ACNGT 1 cut(s) 197
Bst6I CTCTTC 2 cut(s) 114, 182
BstAUI TGTACA 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 2 cut(s) 295, 519
BstDSI CCRYGG 1 cut(s) 453
BstENI CCTNNNNNAGG 1 cut(s) 139
BstKTI GATC 3 cut(s) 52, 304, 612
BstMAI GTCTC 2 cut(s) 55, 116
BstMBI GATC 3 cut(s) 49, 301, 609
BstNSI RCATGY 1 cut(s) 229
BstSFI CTRYAG 1 cut(s) 537
BstSLI GKGCMC 1 cut(s) 421
BstV1I GCAGC 2 cut(s) 484, 595
BstX2I RGATCY 1 cut(s) 609
BstYI RGATCY 1 cut(s) 609
BsuI GTATCC 1 cut(s) 439
BtgI CCRYGG 1 cut(s) 453
BveI ACCTGC 1 cut(s) 72
Cac8I GCNNGC 1 cut(s) 227
CaiI CAGNNNCTG 1 cut(s) 509
CciI TCATGA 1 cut(s) 277
Cfr13I GGNCC 3 cut(s) 28, 84, 214
Csp6I GTAC 1 cut(s) 103
CviAII CATG 4 cut(s) 220, 226, 278, 454
CviJI RGCY 9 cut(s) 145, 205, 350, 400, 497, 523, 563, 586, 601
CviKI_1 RGCY 9 cut(s) 145, 205, 350, 400, 497, 523, 563, 586, 601
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 2 cut(s) 295, 519
DpnI GATC 3 cut(s) 51, 303, 611
DpnII GATC 3 cut(s) 49, 301, 609
DraI TTTAAA 1 cut(s) 265
DrdI GACNNNNNNGTC 1 cut(s) 130
DseDI GACNNNNNNGTC 1 cut(s) 130
Eam1104I CTCTTC 2 cut(s) 114, 182
EarI CTCTTC 2 cut(s) 114, 182
Eco130I CCWWGG 2 cut(s) 87, 453
Eco31I GGTCTC 1 cut(s) 116
Eco47I GGWCC 3 cut(s) 28, 84, 214
Eco57I CTGAAG 2 cut(s) 486, 585
EcoNI CCTNNNNNAGG 1 cut(s) 139
EcoO109I RGGNCCY 1 cut(s) 84
EcoT14I CCWWGG 2 cut(s) 87, 453
ErhI CCWWGG 2 cut(s) 87, 453
FaeI CATG 4 cut(s) 223, 229, 281, 457
FaiI YATR 7 cut(s) 159, 221, 227, 257, 279, 455, 494
FalI AAGNNNNNCTT 1 cut(s) 597
FatI CATG 4 cut(s) 219, 225, 277, 453
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 2 cut(s) 498, 584
Fsp4HI GCNGC 2 cut(s) 498, 584
FspBI CTAG 2 cut(s) 146, 486
GluI GCNGC 2 cut(s) 498, 584
Hin1II CATG 4 cut(s) 223, 229, 281, 457
HinfI GANTC 2 cut(s) 436, 506
Hpy166II GTNNAC 1 cut(s) 541
Hpy188I TCNGA 5 cut(s) 363, 441, 505, 511, 618
Hpy188III TCNNGA 3 cut(s) 179, 278, 379
Hpy8I GTNNAC 1 cut(s) 541
HpyAV CCTTC 3 cut(s) 19, 554, 623
HpyCH4III ACNGT 1 cut(s) 197
HpyCH4IV ACGT 1 cut(s) 321
HpyF3I CTNAG 2 cut(s) 295, 519
HpySE526I ACGT 1 cut(s) 321
Hsp92II CATG 4 cut(s) 223, 229, 281, 457
Kzo9I GATC 3 cut(s) 49, 301, 609
LmnI GCTCC 2 cut(s) 460, 580
LpnPI CCDG 2 cut(s) 67, 251
Lsp1109I GCAGC 2 cut(s) 484, 595
MaeI CTAG 2 cut(s) 146, 486
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 3 cut(s) 54, 322, 481
MalI GATC 3 cut(s) 51, 303, 611
MboI GATC 3 cut(s) 49, 301, 609
MboII GAAGA 5 cut(s) 16, 104, 131, 161, 199
MfeI CAATTG 1 cut(s) 32
MflI RGATCY 1 cut(s) 609
MhlI GDGCHC 1 cut(s) 421
MluCI AATT 5 cut(s) 15, 32, 273, 352, 594
MmeI TCCRAC 1 cut(s) 558
MnlI CCTC 6 cut(s) 183, 334, 367, 390, 431, 451
MseI TTAA 3 cut(s) 264, 306, 597
MslI CAYNNNNRTG 1 cut(s) 224
MunI CAATTG 1 cut(s) 32
NcoI CCATGG 1 cut(s) 453
NdeII GATC 3 cut(s) 49, 301, 609
NlaIII CATG 4 cut(s) 223, 229, 281, 457
NlaIV GGNNCC 1 cut(s) 194
NspI RCATGY 1 cut(s) 229
PaeI GCATGC 1 cut(s) 229
PagI TCATGA 1 cut(s) 277
PfeI GAWTC 2 cut(s) 436, 506
PkrI GCNGC 2 cut(s) 499, 585
PpuMI RGGWCCY 1 cut(s) 84
Psp5II RGGWCCY 1 cut(s) 84
PspN4I GGNNCC 1 cut(s) 194
PspPI GGNCC 3 cut(s) 28, 84, 214
PspPPI RGGWCCY 1 cut(s) 84
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 1 cut(s) 609
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
RseI CAYNNNNRTG 1 cut(s) 224
SaqAI TTAA 3 cut(s) 264, 306, 597
SatI GCNGC 2 cut(s) 498, 584
Sau3AI GATC 3 cut(s) 49, 301, 609
Sau96I GGNCC 3 cut(s) 28, 84, 214
SduI GDGCHC 1 cut(s) 421
SfcI CTRYAG 1 cut(s) 537
SinI GGWCC 3 cut(s) 28, 84, 214
SmiMI CAYNNNNRTG 1 cut(s) 224
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
SpeI ACTAGT 1 cut(s) 485
SphI GCATGC 1 cut(s) 229
Sse9I AATT 5 cut(s) 15, 32, 273, 352, 594
SspMI CTAG 2 cut(s) 146, 486
StyI CCWWGG 2 cut(s) 87, 453
TaaI ACNGT 1 cut(s) 197
TaiI ACGT 1 cut(s) 324
TaqI TCGA 2 cut(s) 68, 339
TaqII GACCGA 1 cut(s) 140
TasI AATT 5 cut(s) 15, 32, 273, 352, 594
TatI WGTACW 1 cut(s) 102
TfiI GAWTC 2 cut(s) 436, 506
Tru1I TTAA 3 cut(s) 264, 306, 597
Tru9I TTAA 3 cut(s) 264, 306, 597
TseI GCWGC 2 cut(s) 497, 583
TspDTI ATGAA 3 cut(s) 17, 266, 294
VpaK11BI GGWCC 3 cut(s) 28, 84, 214
XagI CCTNNNNNAGG 1 cut(s) 139
XceI RCATGY 1 cut(s) 229
XmiI GTMKAC 1 cut(s) 540
XspI CTAG 2 cut(s) 146, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.