Rw0G015830

Microtubule associated protein (MAP65/ASE1 family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00740
OG0001938
Physical Location & Seq
Forward (+)
52767 .. 56203
3437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G015830.1

Sequence Viewer

Length: 363 bp
ATGGACCACATGAGCATGCTAAACTCCCTGTGCTTGGTTCTTGGTATGGATTTTAAACACACAATTCATGAAATCCACCCCACCTTAGATGATCTTAATGGAGAAAAAGATGTAGCGAACAGCACAATCGAGGGTTTGGCTAATTCGGTTCAAATATTGAGAGAGGTCAAGATACAGAGATGGCAGAAGCTTCAAACTTTTGCAAGTGCCCTCTTGGAGATGTGGAATCTGATGGATACAACCATGGAGGAGCAAAAGAAATATCAGAATTTAACTAGTCGCATAGCTGCTTCAGAATCTGAAATCACTGAGCCTAACATTCTTTCTGTAGACCTTCTAAATGAGGAATGGTGGAAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

120

Amino Acids

13.93

Weight (kDa)

4.86

Isoelectric Point (pI)

48.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 5 - 112 6e-11 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 330
AcsI RAATTY 1 cut(s) 268
AcuI CTGAAG 1 cut(s) 276
AfiI CCNNNNNNNGG 1 cut(s) 34
AgsI TTSAA 2 cut(s) 152, 194
AhlI ACTAGT 1 cut(s) 275
AluBI AGCT 2 cut(s) 190, 287
AluI AGCT 2 cut(s) 190, 287
AlwNI CAGNNNCTG 1 cut(s) 299
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 268
AspS9I GGNCC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 4
BaeGI GKGCMC 1 cut(s) 211
BbvI GCAGC 1 cut(s) 274
BccI CCATC 2 cut(s) 174, 226
BciVI GTATCC 1 cut(s) 229
BcuI ACTAGT 1 cut(s) 275
BfaI CTAG 1 cut(s) 276
BfmI CTRYAG 1 cut(s) 327
BfuI GTATCC 1 cut(s) 229
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 1 cut(s) 4
BsaJI CCNNGG 1 cut(s) 243
Bsc4I CCNNNNNNNGG 1 cut(s) 34
BseDI CCNNGG 1 cut(s) 243
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMII CTCAG 1 cut(s) 300
BseRI GAGGAG 1 cut(s) 263
BseSI GKGCMC 1 cut(s) 211
BseXI GCAGC 1 cut(s) 274
BslI CCNNNNNNNGG 1 cut(s) 34
Bsp1286I GDGCHC 1 cut(s) 211
Bsp143I GATC 1 cut(s) 91
Bsp19I CCATGG 1 cut(s) 243
BspCNI CTCAG 1 cut(s) 301
BspHI TCATGA 1 cut(s) 67
BssECI CCNNGG 1 cut(s) 243
BssMI GATC 1 cut(s) 91
BssT1I CCWWGG 1 cut(s) 243
BstC8I GCNNGC 1 cut(s) 17
BstDEI CTNAG 2 cut(s) 85, 309
BstDSI CCRYGG 1 cut(s) 243
BstKTI GATC 1 cut(s) 94
BstMBI GATC 1 cut(s) 91
BstNSI RCATGY 1 cut(s) 19
BstSFI CTRYAG 1 cut(s) 327
BstSLI GKGCMC 1 cut(s) 211
BstV1I GCAGC 1 cut(s) 274
BsuI GTATCC 1 cut(s) 229
BtgI CCRYGG 1 cut(s) 243
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 1 cut(s) 17
CaiI CAGNNNCTG 1 cut(s) 299
CciI TCATGA 1 cut(s) 67
Cfr13I GGNCC 1 cut(s) 4
CviAII CATG 4 cut(s) 10, 16, 68, 244
CviJI RGCY 4 cut(s) 140, 190, 287, 313
CviKI_1 RGCY 4 cut(s) 140, 190, 287, 313
DdeI CTNAG 2 cut(s) 85, 309
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
DraI TTTAAA 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 243
Eco47I GGWCC 1 cut(s) 4
Eco57I CTGAAG 1 cut(s) 276
EcoT14I CCWWGG 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 243
FaeI CATG 4 cut(s) 13, 19, 71, 247
FaiI YATR 6 cut(s) 11, 17, 47, 69, 245, 284
FatI CATG 4 cut(s) 9, 15, 67, 243
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 1 cut(s) 288
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 1 cut(s) 276
GluI GCNGC 1 cut(s) 288
Hin1II CATG 4 cut(s) 13, 19, 71, 247
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 2 cut(s) 226, 296
Hpy166II GTNNAC 1 cut(s) 331
Hpy188I TCNGA 4 cut(s) 231, 267, 295, 301
Hpy188III TCNNGA 2 cut(s) 68, 169
Hpy8I GTNNAC 1 cut(s) 331
HpyAV CCTTC 1 cut(s) 344
HpyCH4V TGCA 1 cut(s) 203
HpyF3I CTNAG 2 cut(s) 85, 309
Hsp92II CATG 4 cut(s) 13, 19, 71, 247
Kzo9I GATC 1 cut(s) 91
LmnI GCTCC 1 cut(s) 250
LpnPI CCDG 1 cut(s) 41
Lsp1109I GCAGC 1 cut(s) 274
MaeI CTAG 1 cut(s) 276
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MhlI GDGCHC 1 cut(s) 211
MluCI AATT 3 cut(s) 63, 142, 268
MnlI CCTC 5 cut(s) 124, 157, 221, 241, 337
MseI TTAA 3 cut(s) 54, 96, 272
MslI CAYNNNNRTG 1 cut(s) 14
NcoI CCATGG 1 cut(s) 243
NdeII GATC 1 cut(s) 91
NlaIII CATG 4 cut(s) 13, 19, 71, 247
NspI RCATGY 1 cut(s) 19
PaeI GCATGC 1 cut(s) 19
PagI TCATGA 1 cut(s) 67
PfeI GAWTC 2 cut(s) 226, 296
PkrI GCNGC 1 cut(s) 289
PspPI GGNCC 1 cut(s) 4
PstNI CAGNNNCTG 1 cut(s) 299
RseI CAYNNNNRTG 1 cut(s) 14
SaqAI TTAA 3 cut(s) 54, 96, 272
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 1 cut(s) 4
SduI GDGCHC 1 cut(s) 211
SetI ASST 5 cut(s) 86, 168, 192, 289, 336
SfcI CTRYAG 1 cut(s) 327
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 1 cut(s) 14
SpeI ACTAGT 1 cut(s) 275
SphI GCATGC 1 cut(s) 19
Sse9I AATT 3 cut(s) 63, 142, 268
SspI AATATT 1 cut(s) 156
SspMI CTAG 1 cut(s) 276
StyI CCWWGG 1 cut(s) 243
TaqI TCGA 1 cut(s) 129
TasI AATT 3 cut(s) 63, 142, 268
TfiI GAWTC 2 cut(s) 226, 296
Tru1I TTAA 3 cut(s) 54, 96, 272
Tru9I TTAA 3 cut(s) 54, 96, 272
TscAI CASTG 1 cut(s) 313
TseI GCWGC 1 cut(s) 287
TspDTI ATGAA 2 cut(s) 56, 84
TspRI CASTG 1 cut(s) 313
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 1 cut(s) 268
XceI RCATGY 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 330
XspI CTAG 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.