Rh1AG227200

microtubule binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001938
Physical Location & Seq
Reverse (-)
43359339 .. 43363487
4149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG227200.1

Sequence Viewer

Length: 1563 bp
ATGCTCAGTCAGCATAGCAGTAATCGGTTTTTGCAGATAGAAACAAAGTCTGGATGTTTTCTGCAGGAACTGCAGAAAATATGGGATGAAGTTGGAGTGCCTAATGTTGAAAGAGATACAATGCTTCTAAAAATCGAGCAGCAGTGCGTGGAGGTGTTTAGGACAAAAGTTAATGAGGCAGAACAGTGTAAAGCTCAACTGCAGCAAGCAATTGCTAATTGGGAAGCAGAGTTTGTAGACATATGTGCTGTCCTGGGTGAGAAACCACCACATTTTGATCATATGATCAGTGGAAGTTTGAAGAAAGAGCTTGAAATCATTATCCCACAATTGGAGGTCATGAAGAAGAGGAAAATTGAAAGGAAGGACCAATTTTTTTCTGTCTTGGATCAGTTACAGAGACTTTCGACTGAAATAAGTAGGTCCTTGGAAGATAATCTGTACAAAATGGTGGTGGAAGAGACCGACTTGTCCTTGAAAAGGCTAGAAGAACTACATAGGCAGTTGCTTGAATATCAACATGAGAAGAGGAACCGTTTGAAGCTGATAATGGACCACATGAGCATGCTAAACTCTCTGTGCTTGGTTCTTGGTATGGATTTTAAACACACAATTCATGAAATCCACCCCACCTTAGATGATCTTAATGGAGAAAAAGACGTAACGAACAGCACAATCGAGGGTTTGGCTAATTCGGTTCAGATATTGAGAGAGGTCAAGATACAGAGATGGCAGAGGAGCGAATTGCTATCAATGCTCAAACAATATAGCATGCTAAGGCAAGAGAAAGAGCAAGAAAAGCAAAGACAGAGAGACCAGAAGAAATTTCAAGGACAACTGCTTGCTGAGAAGGAAGTACTTTATGGGTCAAAACACAGTGCATCTAAGAGTGGGAAGAAGGTTTCCGATCGAAAAATGTCTCTTGGTGGGCCATTAATGCGAAACCAGAAGATTGAGAAAGCTGCTCTACATGTCCACCCTATAAAGAAGGGTGACTCTCTCAATCAAAGGAGCTCTCACCAGCAATACAGTAGAGTAGAAGCTCTATCCTTTGGGAGGAGAGGCTCAGAGTTTTCTGGTCATTCAGTGAAGAAGCAACCATCCACTGCTGCAAAGGCTTGTGAGATGGAGTCGCCATTGATTCGGAAACCCCTTTCTCCTGTCTCTTCCAAAGTGTCATCGAAGGCCAACATTCTAAATTTCCTTGAAGATCAGAAAAGACAACAGAAGAAAAACATCGAAGCAGTACATCTTTTTAAAGAAGTCCCTATCATGTCACCTTCCATTGAAGCAGTACCTCTTTTCAGTGAAGTGCCCATCATGACCCCTTCCAAGCCAATAATCGTCAATGATGAAGAGAACAGTACCCCAAAGACAATGCCAATTCAAGTGCCTCCCACTCCTTCAACAATATCAGTTCATACACTGACAGCCACACCAGCCACACCTTTCACCTCTGGTGCTCACAACAAGGTTGAGAAATCTGGTCGACAAGTTGAATACTCGTTCGAGGAGGTGAGAGCTGGTTTCAACCGTCCTAAATCATACAATGCAGGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

520

Amino Acids

59.47

Weight (kDa)

9.06

Isoelectric Point (pI)

52.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 21 - 248 1.1e-30 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 469
AccI GTMKAC 2 cut(s) 237, 1489
AclWI GGATC 1 cut(s) 396
AcsI RAATTY 2 cut(s) 824, 1198
AfaI GTAC 5 cut(s) 443, 858, 1248, 1296, 1366
AfiI CCNNNNNNNGG 3 cut(s) 331, 480, 1056
AflIII ACRYGT 1 cut(s) 970
AjnI CCWGG 1 cut(s) 252
AluBI AGCT 7 cut(s) 194, 310, 544, 962, 1014, 1043, 1523
AluI AGCT 7 cut(s) 194, 310, 544, 962, 1014, 1043, 1523
Alw21I GWGCWC 2 cut(s) 1016, 1465
Alw26I GTCTC 5 cut(s) 394, 455, 807, 924, 1168
AlwI GGATC 1 cut(s) 396
AlwNI CAGNNNCTG 1 cut(s) 70
AoxI GGCC 2 cut(s) 929, 1185
ApeKI GCWGC 4 cut(s) 139, 202, 962, 1109
ApoI RAATTY 2 cut(s) 824, 1198
AseI ATTAAT 1 cut(s) 935
AspS9I GGNCC 4 cut(s) 367, 423, 553, 929
AsuHPI GGTGA 6 cut(s) 269, 1004, 1010, 1269, 1444, 1528
AvaII GGWCC 3 cut(s) 367, 423, 553
BaeGI GKGCMC 1 cut(s) 1317
BanII GRGCYC 1 cut(s) 1016
Bbv12I GWGCWC 2 cut(s) 1016, 1465
BbvI GCAGC 4 cut(s) 151, 214, 949, 1096
BccI CCATC 4 cut(s) 723, 1108, 1120, 1325
BciT130I CCWGG 1 cut(s) 254
BclI TGATCA 2 cut(s) 277, 285
BcoDI GTCTC 5 cut(s) 394, 455, 807, 924, 1168
BfaI CTAG 1 cut(s) 485
BfmI CTRYAG 3 cut(s) 62, 71, 200
BisI GCNGC 4 cut(s) 140, 203, 963, 1110
BlsI GCNGC 4 cut(s) 141, 204, 964, 1111
BmcAI AGTACT 1 cut(s) 858
Bme1390I CCNGG 1 cut(s) 254
Bme18I GGWCC 3 cut(s) 367, 423, 553
BmgT120I GGNCC 4 cut(s) 367, 423, 553, 929
BmiI GGNNCC 1 cut(s) 533
BmrFI CCNGG 1 cut(s) 254
BmsI GCATC 1 cut(s) 890
Bpu10I CCTNAGC 1 cut(s) 776
BsaI GGTCTC 2 cut(s) 455, 807
BsaJI CCNNGG 2 cut(s) 253, 426
Bsc4I CCNNNNNNNGG 3 cut(s) 331, 480, 1056
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 2 cut(s) 253, 426
BseGI GGATG 3 cut(s) 59, 91, 1100
BseLI CCNNNNNNNGG 3 cut(s) 331, 480, 1056
BseMII CTCAG 3 cut(s) 19, 837, 1080
BseRI GAGGAG 3 cut(s) 751, 1072, 1526
BseSI GKGCMC 1 cut(s) 1317
BseXI GCAGC 4 cut(s) 151, 214, 949, 1096
Bsh1285I CGRYCG 1 cut(s) 910
BshFI GGCC 2 cut(s) 931, 1187
BsiEI CGRYCG 1 cut(s) 910
BsiHKAI GWGCWC 2 cut(s) 1016, 1465
BslFI GGGAC 1 cut(s) 1250
BslI CCNNNNNNNGG 3 cut(s) 331, 480, 1056
BsmAI GTCTC 5 cut(s) 394, 455, 807, 924, 1168
BsmFI GGGAC 1 cut(s) 1250
BsnI GGCC 2 cut(s) 931, 1187
Bso31I GGTCTC 2 cut(s) 455, 807
Bsp1286I GDGCHC 3 cut(s) 1016, 1317, 1465
Bsp1407I TGTACA 1 cut(s) 441
Bsp143I GATC 6 cut(s) 277, 285, 388, 640, 907, 1210
BspANI GGCC 2 cut(s) 931, 1187
BspCNI CTCAG 3 cut(s) 18, 838, 1079
BspHI TCATGA 3 cut(s) 339, 616, 1320
BspLI GGNNCC 1 cut(s) 533
BspMAI CTGCAG 3 cut(s) 66, 75, 204
BspPI GGATC 1 cut(s) 396
BspTNI GGTCTC 2 cut(s) 455, 807
BsrGI TGTACA 1 cut(s) 441
BssECI CCNNGG 2 cut(s) 253, 426
BssMI GATC 6 cut(s) 277, 285, 388, 640, 907, 1210
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 6 cut(s) 186, 536, 878, 1031, 1364, 1535
Bst6I CTCTTC 5 cut(s) 341, 453, 521, 1171, 1350
BstAPI GCANNNNNTGC 1 cut(s) 70
BstAUI TGTACA 1 cut(s) 441
BstC8I GCNNGC 5 cut(s) 207, 566, 773, 843, 1555
BstDEI CTNAG 6 cut(s) 5, 634, 776, 846, 885, 1066
BstENI CCTNNNNNAGG 2 cut(s) 478, 1054
BstF5I GGATG 3 cut(s) 59, 91, 1100
BstKTI GATC 6 cut(s) 280, 288, 391, 643, 910, 1213
BstMAI GTCTC 5 cut(s) 394, 455, 807, 924, 1168
BstMBI GATC 6 cut(s) 277, 285, 388, 640, 907, 1210
BstMCI CGRYCG 1 cut(s) 910
BstMWI GCNNNNNNNGC 7 cut(s) 10, 70, 754, 799, 937, 1115, 1439
BstNI CCWGG 1 cut(s) 254
BstNSI RCATGY 3 cut(s) 568, 775, 974
BstSCI CCNGG 1 cut(s) 252
BstSFI CTRYAG 3 cut(s) 62, 71, 200
BstSLI GKGCMC 1 cut(s) 1317
BstV1I GCAGC 4 cut(s) 151, 214, 949, 1096
BsuRI GGCC 2 cut(s) 931, 1187
BtsCI GGATG 3 cut(s) 59, 91, 1100
BtsI GCAGTG 2 cut(s) 149, 1104
BtsIMutI CAGTG 8 cut(s) 149, 191, 295, 883, 1092, 1104, 1312, 1424
Cac8I GCNNGC 5 cut(s) 207, 566, 773, 843, 1555
CaiI CAGNNNCTG 1 cut(s) 70
CciI TCATGA 3 cut(s) 339, 616, 1320
Cfr13I GGNCC 4 cut(s) 367, 423, 553, 929
Csp6I GTAC 5 cut(s) 442, 857, 1247, 1295, 1365
CviAII CATG 9 cut(s) 340, 521, 559, 565, 617, 772, 971, 1273, 1321
CviQI GTAC 5 cut(s) 442, 857, 1247, 1295, 1365
DdeI CTNAG 6 cut(s) 5, 634, 776, 846, 885, 1066
DpnI GATC 6 cut(s) 279, 287, 390, 642, 909, 1212
DpnII GATC 6 cut(s) 277, 285, 388, 640, 907, 1210
DraI TTTAAA 2 cut(s) 604, 1258
DrdI GACNNNNNNGTC 1 cut(s) 469
DseDI GACNNNNNNGTC 1 cut(s) 469
Eam1104I CTCTTC 5 cut(s) 341, 453, 521, 1171, 1350
EarI CTCTTC 5 cut(s) 341, 453, 521, 1171, 1350
Ecl136II GAGCTC 1 cut(s) 1014
Eco130I CCWWGG 1 cut(s) 426
Eco24I GRGCYC 1 cut(s) 1016
Eco31I GGTCTC 2 cut(s) 455, 807
Eco47I GGWCC 3 cut(s) 367, 423, 553
Eco53kI GAGCTC 1 cut(s) 1014
EcoICRI GAGCTC 1 cut(s) 1014
EcoNI CCTNNNNNAGG 2 cut(s) 478, 1054
EcoO109I RGGNCCY 1 cut(s) 423
EcoRII CCWGG 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 426
EcoT38I GRGCYC 1 cut(s) 1016
ErhI CCWWGG 1 cut(s) 426
FaeI CATG 9 cut(s) 343, 524, 562, 568, 620, 775, 974, 1276, 1324
FaqI GGGAC 1 cut(s) 1250
FatI CATG 9 cut(s) 339, 520, 558, 564, 616, 771, 970, 1272, 1320
FauNDI CATATG 2 cut(s) 242, 282
FbaI TGATCA 2 cut(s) 277, 285
FblI GTMKAC 2 cut(s) 237, 1489
Fnu4HI GCNGC 4 cut(s) 140, 203, 963, 1110
FokI GGATG 3 cut(s) 66, 98, 1087
FriOI GRGCYC 1 cut(s) 1016
Fsp4HI GCNGC 4 cut(s) 140, 203, 963, 1110
FspBI CTAG 1 cut(s) 485
GluI GCNGC 4 cut(s) 140, 203, 963, 1110
HaeIII GGCC 2 cut(s) 931, 1187
Hin1II CATG 9 cut(s) 343, 524, 562, 568, 620, 775, 974, 1276, 1324
HincII GTYRAC 1 cut(s) 1490
HindII GTYRAC 1 cut(s) 1490
HinfI GANTC 3 cut(s) 995, 1130, 1141
HphI GGTGA 6 cut(s) 269, 1004, 1010, 1269, 1444, 1528
Hpy166II GTNNAC 3 cut(s) 238, 976, 1490
Hpy188I TCNGA 5 cut(s) 702, 907, 1069, 1146, 1215
Hpy188III TCNNGA 5 cut(s) 51, 340, 617, 718, 1321
Hpy8I GTNNAC 3 cut(s) 238, 976, 1490
HpyAV CCTTC 8 cut(s) 358, 844, 892, 982, 1177, 1290, 1338, 1413
HpyCH4III ACNGT 6 cut(s) 186, 536, 878, 1031, 1364, 1535
HpyCH4IV ACGT 1 cut(s) 660
HpyCH4V TGCA 7 cut(s) 34, 64, 73, 202, 881, 1112, 1553
HpyF10VI GCNNNNNNNGC 7 cut(s) 10, 70, 754, 799, 937, 1115, 1439
HpyF3I CTNAG 6 cut(s) 5, 634, 776, 846, 885, 1066
HpySE526I ACGT 1 cut(s) 660
Hsp92II CATG 9 cut(s) 343, 524, 562, 568, 620, 775, 974, 1276, 1324
Ksp22I TGATCA 2 cut(s) 277, 285
Kzo9I GATC 6 cut(s) 277, 285, 388, 640, 907, 1210
LmnI GCTCC 2 cut(s) 738, 1011
Lsp1109I GCAGC 4 cut(s) 151, 214, 949, 1096
LweI GCATC 1 cut(s) 890
MaeI CTAG 1 cut(s) 485
MaeII ACGT 1 cut(s) 660
MaeIII GTNAC 4 cut(s) 393, 661, 992, 1275
MalI GATC 6 cut(s) 279, 287, 390, 642, 909, 1212
MboI GATC 6 cut(s) 277, 285, 388, 640, 907, 1210
MfeI CAATTG 2 cut(s) 210, 329
MhlI GDGCHC 3 cut(s) 1016, 1317, 1465
MlyI GAGTC 2 cut(s) 989, 1139
MmeI TCCRAC 1 cut(s) 73
MseI TTAA 5 cut(s) 171, 603, 645, 935, 1257
MslI CAYNNNNRTG 1 cut(s) 563
MspR9I CCNGG 1 cut(s) 254
MunI CAATTG 2 cut(s) 210, 329
MvaI CCWGG 1 cut(s) 254
MwoI GCNNNNNNNGC 7 cut(s) 10, 70, 754, 799, 937, 1115, 1439
NdeI CATATG 2 cut(s) 242, 282
NdeII GATC 6 cut(s) 277, 285, 388, 640, 907, 1210
NlaIII CATG 9 cut(s) 343, 524, 562, 568, 620, 775, 974, 1276, 1324
NlaIV GGNNCC 1 cut(s) 533
NmuCI GTSAC 2 cut(s) 992, 1275
NspI RCATGY 3 cut(s) 568, 775, 974
PaeI GCATGC 2 cut(s) 568, 775
PagI TCATGA 3 cut(s) 339, 616, 1320
PciI ACATGT 1 cut(s) 970
PfeI GAWTC 1 cut(s) 1141
PkrI GCNGC 4 cut(s) 141, 204, 964, 1111
Ple19I CGATCG 1 cut(s) 910
PleI GAGTC 2 cut(s) 989, 1138
PpsI GAGTC 2 cut(s) 989, 1138
PpuMI RGGWCCY 1 cut(s) 423
PscI ACATGT 1 cut(s) 970
PshBI ATTAAT 1 cut(s) 935
Psp124BI GAGCTC 1 cut(s) 1016
Psp5II RGGWCCY 1 cut(s) 423
Psp6I CCWGG 1 cut(s) 252
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 1 cut(s) 533
PspPI GGNCC 4 cut(s) 367, 423, 553, 929
PspPPI RGGWCCY 1 cut(s) 423
PstI CTGCAG 3 cut(s) 66, 75, 204
PstNI CAGNNNCTG 1 cut(s) 70
PvuI CGATCG 1 cut(s) 910
RsaI GTAC 5 cut(s) 443, 858, 1248, 1296, 1366
RsaNI GTAC 5 cut(s) 442, 857, 1247, 1295, 1365
RseI CAYNNNNRTG 1 cut(s) 563
SacI GAGCTC 1 cut(s) 1016
SalI GTCGAC 1 cut(s) 1488
SaqAI TTAA 5 cut(s) 171, 603, 645, 935, 1257
SatI GCNGC 4 cut(s) 140, 203, 963, 1110
Sau3AI GATC 6 cut(s) 277, 285, 388, 640, 907, 1210
Sau96I GGNCC 4 cut(s) 367, 423, 553, 929
ScaI AGTACT 1 cut(s) 858
SchI GAGTC 2 cut(s) 989, 1139
ScrFI CCNGG 1 cut(s) 254
SduI GDGCHC 3 cut(s) 1016, 1317, 1465
SfaNI GCATC 1 cut(s) 890
SfcI CTRYAG 3 cut(s) 62, 71, 200
SinI GGWCC 3 cut(s) 367, 423, 553
SmiMI CAYNNNNRTG 1 cut(s) 563
SphI GCATGC 2 cut(s) 568, 775
SspMI CTAG 1 cut(s) 485
SstI GAGCTC 1 cut(s) 1016
StyD4I CCNGG 1 cut(s) 252
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 6 cut(s) 186, 536, 878, 1031, 1364, 1535
TaiI ACGT 1 cut(s) 663
TaqI TCGA 8 cut(s) 135, 407, 678, 910, 1181, 1239, 1489, 1509
TaqII GACCGA 1 cut(s) 479
TatI WGTACW 3 cut(s) 441, 856, 1246
TfiI GAWTC 1 cut(s) 1141
Tru1I TTAA 5 cut(s) 171, 603, 645, 935, 1257
Tru9I TTAA 5 cut(s) 171, 603, 645, 935, 1257
TscAI CASTG 8 cut(s) 149, 191, 295, 883, 1092, 1111, 1312, 1431
TseFI GTSAC 2 cut(s) 992, 1275
TseI GCWGC 4 cut(s) 139, 202, 962, 1109
Tsp45I GTSAC 2 cut(s) 992, 1275
TspDTI ATGAA 6 cut(s) 102, 356, 605, 633, 1368, 1409
TspRI CASTG 8 cut(s) 149, 191, 295, 883, 1092, 1111, 1312, 1431
VpaK11BI GGWCC 3 cut(s) 367, 423, 553
VspI ATTAAT 1 cut(s) 935
XagI CCTNNNNNAGG 2 cut(s) 478, 1054
XapI RAATTY 2 cut(s) 824, 1198
XceI RCATGY 3 cut(s) 568, 775, 974
XmiI GTMKAC 2 cut(s) 237, 1489
XspI CTAG 1 cut(s) 485
ZrmI AGTACT 1 cut(s) 858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.