RchiOBHm_Chr7g0208631

microtubule binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001938
Physical Location & Seq
Reverse (-)
26017116 .. 26020440
3325 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ18673

Sequence Viewer

Length: 549 bp
ATGTGGAATCTGATGGATACAACCATGGAGGAGCAAAAGAAATATCAGAATGTAACTAGTCGCATAGCTACTTTAGAATTTGAAATCACTGAGCCTAACATTCTTTCTGTAGACCTGCTAAATGATGTTGAAGCTGAAGTGTCAAAGTTGGAGCAGCTCAAGTCAATTAAGCTGAAAGAGATCCTTCTGAAAAAGAAGTTGGAACTGGAGGAGTTATGCAGACAGTCTCACATGGTTACAGAAATACTGAGTGCTGCAGAATACTCAAATGAAGCTATAGAGTCTGGAGTTGTGGACCCTGCATGCCTGTTGGAACAAATTGAGCTTCAAATTGCAAGGGCAAAAGAGGAAGCTCTAAGCAGGAAAGAAATACTAGAAAAGATTGAAAAGTGGTTGGTTGCTTGTCAAGAAGAATACTGGCTAGAGGAATACAATAGGGATGATAATCGCTATACAGCAGGAAGAGATGCTCATATTACTTTGAAACGTGCAGAGAAAGTGCGTGTTTTAGTAAACAAAATTCCCGGTAAAGACTTGGTTAAATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

182

Amino Acids

21.18

Weight (kDa)

4.98

Isoelectric Point (pI)

55.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 1 - 176 3.4e-35 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 547
Acc36I ACCTGC 1 cut(s) 123
AccI GTMKAC 1 cut(s) 111
AclWI GGATC 1 cut(s) 175
AcsI RAATTY 2 cut(s) 77, 519
AcuI CTGAAG 1 cut(s) 156
AgsI TTSAA 5 cut(s) 83, 131, 329, 386, 484
AhlI ACTAGT 1 cut(s) 56
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
AluBI AGCT 7 cut(s) 68, 134, 157, 172, 275, 325, 353
AluI AGCT 7 cut(s) 68, 134, 157, 172, 275, 325, 353
Alw26I GTCTC 1 cut(s) 231
AlwI GGATC 1 cut(s) 175
ApeKI GCWGC 2 cut(s) 154, 254
ApoI RAATTY 2 cut(s) 77, 519
AspS9I GGNCC 1 cut(s) 295
AsuC2I CCSGG 1 cut(s) 525
AvaII GGWCC 1 cut(s) 295
BbvI GCAGC 2 cut(s) 166, 241
BccI CCATC 1 cut(s) 7
BciVI GTATCC 1 cut(s) 10
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 1 cut(s) 231
BcuI ACTAGT 1 cut(s) 56
BfaI CTAG 3 cut(s) 57, 374, 422
BfmI CTRYAG 3 cut(s) 108, 255, 276
BfuAI ACCTGC 1 cut(s) 123
BfuI GTATCC 1 cut(s) 10
BisI GCNGC 2 cut(s) 155, 255
BlsI GCNGC 2 cut(s) 156, 256
Bme1390I CCNGG 1 cut(s) 525
Bme18I GGWCC 1 cut(s) 295
BmgT120I GGNCC 1 cut(s) 295
BmiI GGNNCC 1 cut(s) 297
BmrFI CCNGG 1 cut(s) 525
BmsI GCATC 1 cut(s) 457
BpmI CTGGAG 2 cut(s) 227, 306
BpuEI CTTGAG 1 cut(s) 143
BpuMI CCSGG 1 cut(s) 525
BsaBI GATNNNNATC 1 cut(s) 444
BsaJI CCNNGG 1 cut(s) 24
Bse1I ACTGG 2 cut(s) 210, 422
Bse8I GATNNNNATC 1 cut(s) 444
BseDI CCNNGG 1 cut(s) 24
BseGI GGATG 1 cut(s) 445
BseJI GATNNNNATC 1 cut(s) 444
BseMII CTCAG 2 cut(s) 81, 239
BseNI ACTGG 2 cut(s) 210, 422
BseRI GAGGAG 2 cut(s) 44, 224
BseXI GCAGC 2 cut(s) 166, 241
BsgI GTGCAG 1 cut(s) 510
BsiSI CCGG 1 cut(s) 525
BsmAI GTCTC 1 cut(s) 231
Bsp143I GATC 1 cut(s) 180
Bsp19I CCATGG 1 cut(s) 24
BspCNI CTCAG 2 cut(s) 82, 240
BspLI GGNNCC 1 cut(s) 297
BspMAI CTGCAG 1 cut(s) 259
BspMI ACCTGC 1 cut(s) 123
BspPI GGATC 1 cut(s) 175
BsrI ACTGG 2 cut(s) 210, 422
BssECI CCNNGG 1 cut(s) 24
BssMI GATC 1 cut(s) 180
BssT1I CCWWGG 1 cut(s) 24
Bst4CI ACNGT 1 cut(s) 225
Bst6I CTCTTC 1 cut(s) 457
BstC8I GCNNGC 1 cut(s) 304
BstDEI CTNAG 3 cut(s) 90, 248, 356
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 445
BstKTI GATC 1 cut(s) 183
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 1 cut(s) 180
BstNSI RCATGY 1 cut(s) 306
BstSCI CCNGG 1 cut(s) 523
BstSFI CTRYAG 3 cut(s) 108, 255, 276
BstV1I GCAGC 2 cut(s) 166, 241
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BsuI GTATCC 1 cut(s) 10
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 1 cut(s) 445
BtsIMutI CAGTG 1 cut(s) 87
BveI ACCTGC 1 cut(s) 123
Cac8I GCNNGC 1 cut(s) 304
Cfr13I GGNCC 1 cut(s) 295
CviAII CATG 3 cut(s) 25, 232, 303
CviJI RGCY 9 cut(s) 68, 94, 134, 157, 172, 275, 325, 353, 421
CviKI_1 RGCY 9 cut(s) 68, 94, 134, 157, 172, 275, 325, 353, 421
DdeI CTNAG 3 cut(s) 90, 248, 356
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
Eam1104I CTCTTC 1 cut(s) 457
EarI CTCTTC 1 cut(s) 457
Eco130I CCWWGG 1 cut(s) 24
Eco47I GGWCC 1 cut(s) 295
Eco57I CTGAAG 1 cut(s) 156
EcoT14I CCWWGG 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 24
FaeI CATG 3 cut(s) 28, 235, 306
FaiI YATR 9 cut(s) 26, 65, 217, 233, 278, 304, 453, 474, 547
FalI AAGNNNNNCTT 2 cut(s) 168, 200
FatI CATG 3 cut(s) 24, 231, 302
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 2 cut(s) 155, 255
FokI GGATG 1 cut(s) 452
Fsp4HI GCNGC 2 cut(s) 155, 255
FspBI CTAG 3 cut(s) 57, 374, 422
GluI GCNGC 2 cut(s) 155, 255
GsuI CTGGAG 2 cut(s) 227, 306
HapII CCGG 1 cut(s) 525
Hin1II CATG 3 cut(s) 28, 235, 306
HinfI GANTC 2 cut(s) 7, 281
HpaII CCGG 1 cut(s) 525
Hpy166II GTNNAC 3 cut(s) 112, 295, 514
Hpy188I TCNGA 3 cut(s) 12, 48, 189
Hpy188III TCNNGA 2 cut(s) 285, 407
Hpy8I GTNNAC 3 cut(s) 112, 295, 514
HpyAV CCTTC 1 cut(s) 194
HpyCH4III ACNGT 1 cut(s) 225
HpyCH4IV ACGT 1 cut(s) 487
HpyCH4V TGCA 5 cut(s) 219, 257, 302, 335, 491
HpyF3I CTNAG 3 cut(s) 90, 248, 356
HpySE526I ACGT 1 cut(s) 487
Hsp92II CATG 3 cut(s) 28, 235, 306
Kzo9I GATC 1 cut(s) 180
LmnI GCTCC 2 cut(s) 31, 151
LpnPI CCDG 9 cut(s) 128, 191, 270, 312, 320, 346, 403, 444, 538
Lsp1109I GCAGC 2 cut(s) 166, 241
LweI GCATC 1 cut(s) 457
MaeI CTAG 3 cut(s) 57, 374, 422
MaeII ACGT 1 cut(s) 487
MaeIII GTNAC 2 cut(s) 52, 235
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 2 cut(s) 422, 474
MflI RGATCY 1 cut(s) 180
MluCI AATT 6 cut(s) 77, 165, 318, 330, 519, 542
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 3 cut(s) 129, 180, 291
MnlI CCTC 4 cut(s) 22, 202, 340, 418
MseI TTAA 2 cut(s) 168, 540
MspI CCGG 1 cut(s) 525
MspR9I CCNGG 1 cut(s) 525
NciI CCSGG 1 cut(s) 525
NcoI CCATGG 1 cut(s) 24
NdeII GATC 1 cut(s) 180
NlaIII CATG 3 cut(s) 28, 235, 306
NlaIV GGNNCC 1 cut(s) 297
NspI RCATGY 1 cut(s) 306
PaeI GCATGC 1 cut(s) 306
PfeI GAWTC 1 cut(s) 7
PkrI GCNGC 2 cut(s) 156, 256
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
PsiI TTATAA 1 cut(s) 547
PspN4I GGNNCC 1 cut(s) 297
PspPI GGNCC 1 cut(s) 295
PstI CTGCAG 1 cut(s) 259
PsuI RGATCY 1 cut(s) 180
SaqAI TTAA 2 cut(s) 168, 540
SatI GCNGC 2 cut(s) 155, 255
Sau3AI GATC 1 cut(s) 180
Sau96I GGNCC 1 cut(s) 295
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 1 cut(s) 525
SetI ASST 9 cut(s) 70, 117, 136, 159, 174, 277, 327, 355, 490
SfaNI GCATC 1 cut(s) 457
SfcI CTRYAG 3 cut(s) 108, 255, 276
SinI GGWCC 1 cut(s) 295
SmlI CTYRAG 1 cut(s) 158
SmoI CTYRAG 1 cut(s) 158
SpeI ACTAGT 1 cut(s) 56
SphI GCATGC 1 cut(s) 306
Sse9I AATT 6 cut(s) 77, 165, 318, 330, 519, 542
SspMI CTAG 3 cut(s) 57, 374, 422
StyD4I CCNGG 1 cut(s) 523
StyI CCWWGG 1 cut(s) 24
TaaI ACNGT 1 cut(s) 225
TaiI ACGT 1 cut(s) 490
TasI AATT 6 cut(s) 77, 165, 318, 330, 519, 542
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 2 cut(s) 168, 540
Tru9I TTAA 2 cut(s) 168, 540
TscAI CASTG 1 cut(s) 94
TseI GCWGC 2 cut(s) 154, 254
TspDTI ATGAA 1 cut(s) 285
TspRI CASTG 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 295
XapI RAATTY 2 cut(s) 77, 519
XceI RCATGY 1 cut(s) 306
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 3 cut(s) 57, 374, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.