Rh2AG170800

Microtubule associated protein (MAP65/ASE1 family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001938
Physical Location & Seq
Forward (+)
16242375 .. 16247428
5054 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG170800.1

Sequence Viewer

Length: 465 bp
ATGGTGGTGGAAGAGACCGACTTGTCCTTGAAAAGGCTAGAAGAACTACATAGGCAGTTGCGTGAATATCAAGATGAGAAGAGGAACTGTTTGAAGCTGATAATGGACCACATGAGCATGCTAAACTCCCTGTGCTTGGTTCTTGGTATGGATTTTAAAGACACAATTCATGAAATCGACCCCACCTTAGATGATCTTAATGGAGAAAAAGACGTAACGAACAACACAATCGAGGGTTTGGCTAATTCGGTTCAAATATTGGGAGAGGTCAAGATACAGAGATGGCAGAGGCTTCAAACTTTCGCAAGTGCCCTCTTGGAGATGTGGAATCTGATGGATACAACCATGGAGGAGCAAAAGAAATATCAGAATGTAACTAGTCGCATAGCTGCTTCAGAATCTGAAATCACTGAGCCTAAAATTCTTTATGTAGACCTTCTAAATGATGAATGGTGGAAACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

154

Amino Acids

18.14

Weight (kDa)

4.71

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 4 - 133 6.3e-15 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 22
AccI GTMKAC 1 cut(s) 432
AcsI RAATTY 1 cut(s) 420
AcuI CTGAAG 1 cut(s) 378
AfiI CCNNNNNNNGG 2 cut(s) 33, 136
AgsI TTSAA 4 cut(s) 31, 94, 254, 296
AhlI ACTAGT 1 cut(s) 377
AluBI AGCT 2 cut(s) 97, 389
AluI AGCT 2 cut(s) 97, 389
Alw26I GTCTC 1 cut(s) 8
AlwNI CAGNNNCTG 1 cut(s) 401
ApeKI GCWGC 1 cut(s) 389
ApoI RAATTY 1 cut(s) 420
AspS9I GGNCC 1 cut(s) 106
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 313
BbvI GCAGC 1 cut(s) 376
BccI CCATC 2 cut(s) 276, 328
BciVI GTATCC 1 cut(s) 331
BcoDI GTCTC 1 cut(s) 8
BcuI ACTAGT 1 cut(s) 377
BfaI CTAG 2 cut(s) 38, 378
BfuI GTATCC 1 cut(s) 331
BisI GCNGC 1 cut(s) 390
BlsI GCNGC 1 cut(s) 391
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 106
BsaI GGTCTC 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 345
Bsc4I CCNNNNNNNGG 2 cut(s) 33, 136
BseDI CCNNGG 1 cut(s) 345
BseLI CCNNNNNNNGG 2 cut(s) 33, 136
BseMII CTCAG 1 cut(s) 402
BseRI GAGGAG 1 cut(s) 365
BseSI GKGCMC 1 cut(s) 313
BseXI GCAGC 1 cut(s) 376
BslI CCNNNNNNNGG 2 cut(s) 33, 136
BsmAI GTCTC 1 cut(s) 8
Bso31I GGTCTC 1 cut(s) 8
Bsp1286I GDGCHC 1 cut(s) 313
Bsp143I GATC 1 cut(s) 193
Bsp19I CCATGG 1 cut(s) 345
BspCNI CTCAG 1 cut(s) 403
BspHI TCATGA 1 cut(s) 169
BspTNI GGTCTC 1 cut(s) 8
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 1 cut(s) 193
BssT1I CCWWGG 1 cut(s) 345
Bst4CI ACNGT 1 cut(s) 89
Bst6I CTCTTC 2 cut(s) 6, 74
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 2 cut(s) 187, 411
BstDSI CCRYGG 1 cut(s) 345
BstENI CCTNNNNNAGG 1 cut(s) 31
BstKTI GATC 1 cut(s) 196
BstMAI GTCTC 1 cut(s) 8
BstMBI GATC 1 cut(s) 193
BstNSI RCATGY 1 cut(s) 121
BstSLI GKGCMC 1 cut(s) 313
BstV1I GCAGC 1 cut(s) 376
BsuI GTATCC 1 cut(s) 331
BtgI CCRYGG 1 cut(s) 345
BtsIMutI CAGTG 1 cut(s) 408
Cac8I GCNNGC 1 cut(s) 119
CaiI CAGNNNCTG 1 cut(s) 401
CciI TCATGA 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 106
CviAII CATG 4 cut(s) 112, 118, 170, 346
CviJI RGCY 6 cut(s) 37, 97, 242, 292, 389, 415
CviKI_1 RGCY 6 cut(s) 37, 97, 242, 292, 389, 415
DdeI CTNAG 2 cut(s) 187, 411
DpnI GATC 1 cut(s) 195
DpnII GATC 1 cut(s) 193
DraI TTTAAA 1 cut(s) 157
DrdI GACNNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 1 cut(s) 22
Eam1104I CTCTTC 2 cut(s) 6, 74
EarI CTCTTC 2 cut(s) 6, 74
Eco130I CCWWGG 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 378
EcoNI CCTNNNNNAGG 1 cut(s) 31
EcoT14I CCWWGG 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 4 cut(s) 115, 121, 173, 349
FaiI YATR 8 cut(s) 51, 113, 119, 149, 171, 347, 386, 429
FatI CATG 4 cut(s) 111, 117, 169, 345
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 1 cut(s) 390
Fsp4HI GCNGC 1 cut(s) 390
FspBI CTAG 2 cut(s) 38, 378
GluI GCNGC 1 cut(s) 390
Hin1II CATG 4 cut(s) 115, 121, 173, 349
HinfI GANTC 2 cut(s) 328, 398
Hpy166II GTNNAC 1 cut(s) 433
Hpy188I TCNGA 4 cut(s) 333, 369, 397, 403
Hpy188III TCNNGA 3 cut(s) 71, 170, 271
Hpy8I GTNNAC 1 cut(s) 433
HpyAV CCTTC 1 cut(s) 446
HpyCH4III ACNGT 1 cut(s) 89
HpyCH4IV ACGT 1 cut(s) 213
HpyF3I CTNAG 2 cut(s) 187, 411
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 4 cut(s) 115, 121, 173, 349
Kzo9I GATC 1 cut(s) 193
LmnI GCTCC 1 cut(s) 352
LpnPI CCDG 1 cut(s) 143
Lsp1109I GCAGC 1 cut(s) 376
MaeI CTAG 2 cut(s) 38, 378
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 2 cut(s) 214, 373
MalI GATC 1 cut(s) 195
MboI GATC 1 cut(s) 193
MboII GAAGA 3 cut(s) 23, 53, 91
MhlI GDGCHC 1 cut(s) 313
MluCI AATT 3 cut(s) 165, 244, 420
MnlI CCTC 6 cut(s) 75, 226, 259, 282, 323, 343
MseI TTAA 2 cut(s) 156, 198
MslI CAYNNNNRTG 1 cut(s) 116
NcoI CCATGG 1 cut(s) 345
NdeII GATC 1 cut(s) 193
NlaIII CATG 4 cut(s) 115, 121, 173, 349
NspI RCATGY 1 cut(s) 121
PaeI GCATGC 1 cut(s) 121
PagI TCATGA 1 cut(s) 169
PfeI GAWTC 2 cut(s) 328, 398
PkrI GCNGC 1 cut(s) 391
PspPI GGNCC 1 cut(s) 106
PstNI CAGNNNCTG 1 cut(s) 401
RseI CAYNNNNRTG 1 cut(s) 116
SaqAI TTAA 2 cut(s) 156, 198
SatI GCNGC 1 cut(s) 390
Sau3AI GATC 1 cut(s) 193
Sau96I GGNCC 1 cut(s) 106
SduI GDGCHC 1 cut(s) 313
SetI ASST 6 cut(s) 99, 188, 216, 270, 391, 438
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 1 cut(s) 116
SpeI ACTAGT 1 cut(s) 377
SphI GCATGC 1 cut(s) 121
Sse9I AATT 3 cut(s) 165, 244, 420
SspI AATATT 1 cut(s) 258
SspMI CTAG 2 cut(s) 38, 378
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 1 cut(s) 89
TaiI ACGT 1 cut(s) 216
TaqI TCGA 2 cut(s) 177, 231
TaqII GACCGA 1 cut(s) 32
TasI AATT 3 cut(s) 165, 244, 420
TfiI GAWTC 2 cut(s) 328, 398
Tru1I TTAA 2 cut(s) 156, 198
Tru9I TTAA 2 cut(s) 156, 198
TscAI CASTG 1 cut(s) 415
TseI GCWGC 1 cut(s) 389
TspDTI ATGAA 3 cut(s) 158, 186, 462
TspRI CASTG 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 106
XagI CCTNNNNNAGG 1 cut(s) 31
XapI RAATTY 1 cut(s) 420
XceI RCATGY 1 cut(s) 121
XmiI GTMKAC 1 cut(s) 432
XspI CTAG 2 cut(s) 38, 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.