Rh1DG224700

microtubule binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001938
Physical Location & Seq
Reverse (-)
43526187 .. 43531178
4992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG224700.1

Sequence Viewer

Length: 1488 bp
ATGTTCAGTCAGAGTAGCAGTAATCGGTTTTTGCAGATAGAAACAAAGTCTGGATGTTTTCTGCAGGAACTGCAGAAAATATGGGATGAAGTAGGAGTGCCTAATGCTGAAAGGGATACAATGCTTTTAAAAATTGAGCAGCAGTGCGCGGAGGTGTTTAGGACAAAAGTTGATGAGGCGGAGCAGTGTAAAGCTCAACTGCAGCAAGCAATTGCTAATTGTGAAGCAGAGTTTCTAGACATATGTGCTGTTCTGGGTGAGAAACCACCAGATTTTGATCATAAGATCAGTGGAAGTTTGAAGAAAGAGCTTGAAATCATTATCCCACAATTGGAGGTCATGAAGAAGCAGAAAATTGAAAGGAAGGACCACTTTTTTTCTGTCTTGGATCAGTTACAGAGACTTTCGACTGAAATAAGCAGGTCTTTGGAAGATAATCTGTACAAAATGGTGGTGGAAGAGACCGACTTGTCCTTGAAAAGGCTAGAAGAACTACATAGGCAGTTGCTTGAATATCAAGATGAGAAGAGGAACCGTTTGAAGCTGATAATGGACCACATGAACATGCTAAACTCTCTGTGCTTGGTTCTTGGTATGGATTTTAAACACACAATTCATGAAATCCACCCCACCTTAGATGATCTTAATGGAGAAAAAGACGTAACGAACAGCACAATCGAGGGTTTGGCTAATTCGGTTCAGATATTGAGAGAGGTCAAGATACAGAGATGGCAGAGGGACCAGAAAAAATTTCAAGGACAACTGCTAGCTGAGAAGGAAGTACTTTATGGGTCAAAACACAGTGCATCTAAGAGTGGGAAGAAGGTTTCCGATCGAAAAATGTCTCTTGGTGGGCCATTAATGCGAAACCAGAAGATTGAGAAAGCTGCTCTACATGTCCACCCTATAAAGAAGGGTGACTCTCTCAATCAAAGGAGCTCTCACCAGCAATACAGTGGAGTAGAAGCTCTATCCTTTGGGAGGAGAGGCTCAGAGTTTTCTGGTCATTCAGTGAAGAAGCAACCATCCACTGCTGCAAAGGCTAGTGAGATGGAGTCGCCATTGATTCGGAAACCCCTTTCTCCTGTCTCTTCCAAAGTGTCATCGAAGGCCAACATTCTAAATTTCCTTGAAGATCAGAAAAGACAACAGAAGAAAAACATCGAAGCAGTACATCTTTTTAAAGAAGTCCCTATCATGTCACCTTCCATTGAAGCAGTACCTCTTTTCAGTGAAGTGCCCGTCATGACCCCTTCCAAGCCAATAATCGTCAATGATGAAGAGAACAGTACCCCAAAGACAATGCCAATTCAAGTGCCTCCCACTCCTTCAACAATATCAGTTCATACACTGACAGCCACACCAGCTACACCTTTCACCTCTGGTGCTCACAACAAGGTTGAGAAATCTGGTCGACAAGTTGAATACTCGTTTGAGGAGGTGAGAGCTGGTTTCAACCGTCCTAAATCACACAATGCAGGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

495

Amino Acids

55.98

Weight (kDa)

8.51

Isoelectric Point (pI)

51.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 21 - 246 3.8e-32 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 469
Acc36I ACCTGC 1 cut(s) 411
AccI GTMKAC 1 cut(s) 1414
AccII CGCG 1 cut(s) 149
AciI CCGC 2 cut(s) 149, 179
AclWI GGATC 1 cut(s) 396
AcsI RAATTY 2 cut(s) 749, 1123
AfaI GTAC 5 cut(s) 443, 783, 1173, 1221, 1291
AfiI CCNNNNNNNGG 3 cut(s) 331, 480, 981
AflIII ACRYGT 1 cut(s) 895
AluBI AGCT 9 cut(s) 194, 310, 544, 770, 887, 939, 968, 1367, 1448
AluI AGCT 9 cut(s) 194, 310, 544, 770, 887, 939, 968, 1367, 1448
Alw21I GWGCWC 2 cut(s) 941, 1390
Alw26I GTCTC 4 cut(s) 394, 455, 849, 1093
AlwI GGATC 1 cut(s) 396
AlwNI CAGNNNCTG 1 cut(s) 70
AoxI GGCC 2 cut(s) 854, 1110
ApeKI GCWGC 4 cut(s) 139, 202, 887, 1034
ApoI RAATTY 2 cut(s) 749, 1123
AseI ATTAAT 1 cut(s) 860
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 4 cut(s) 367, 553, 739, 854
AsuHPI GGTGA 6 cut(s) 269, 929, 935, 1194, 1369, 1453
AsuNHI GCTAGC 1 cut(s) 766
AvaII GGWCC 3 cut(s) 367, 553, 739
BaeGI GKGCMC 1 cut(s) 1242
BanII GRGCYC 1 cut(s) 941
Bbv12I GWGCWC 2 cut(s) 941, 1390
BbvI GCAGC 4 cut(s) 151, 214, 874, 1021
BccI CCATC 3 cut(s) 723, 1033, 1045
BciVI GTATCC 1 cut(s) 109
BclI TGATCA 1 cut(s) 277
BcoDI GTCTC 4 cut(s) 394, 455, 849, 1093
BfaI CTAG 4 cut(s) 236, 485, 767, 1044
BfmI CTRYAG 3 cut(s) 62, 71, 200
BfuAI ACCTGC 1 cut(s) 411
BfuI GTATCC 1 cut(s) 109
BisI GCNGC 4 cut(s) 140, 203, 888, 1035
BlsI GCNGC 4 cut(s) 141, 204, 889, 1036
BmcAI AGTACT 1 cut(s) 783
Bme18I GGWCC 3 cut(s) 367, 553, 739
BmgT120I GGNCC 4 cut(s) 367, 553, 739, 854
BmiI GGNNCC 2 cut(s) 533, 740
BmsI GCATC 1 cut(s) 815
BmtI GCTAGC 1 cut(s) 770
BsaBI GATNNNNATC 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 455
Bsc4I CCNNNNNNNGG 3 cut(s) 331, 480, 981
Bse8I GATNNNNATC 1 cut(s) 276
BseGI GGATG 3 cut(s) 59, 91, 1025
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 3 cut(s) 331, 480, 981
BseMII CTCAG 2 cut(s) 762, 1005
BseRI GAGGAG 2 cut(s) 997, 1451
BseSI GKGCMC 1 cut(s) 1242
BseXI GCAGC 4 cut(s) 151, 214, 874, 1021
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 1 cut(s) 835
BshFI GGCC 2 cut(s) 856, 1112
BsiEI CGRYCG 1 cut(s) 835
BsiHKAI GWGCWC 2 cut(s) 941, 1390
BslFI GGGAC 2 cut(s) 752, 1175
BslI CCNNNNNNNGG 3 cut(s) 331, 480, 981
BsmAI GTCTC 4 cut(s) 394, 455, 849, 1093
BsmFI GGGAC 2 cut(s) 752, 1175
BsnI GGCC 2 cut(s) 856, 1112
Bso31I GGTCTC 1 cut(s) 455
Bsp1286I GDGCHC 3 cut(s) 941, 1242, 1390
Bsp1407I TGTACA 1 cut(s) 441
Bsp143I GATC 6 cut(s) 277, 285, 388, 640, 832, 1135
BspACI CCGC 2 cut(s) 149, 179
BspANI GGCC 2 cut(s) 856, 1112
BspCNI CTCAG 2 cut(s) 763, 1004
BspFNI CGCG 1 cut(s) 149
BspHI TCATGA 3 cut(s) 339, 616, 1245
BspLI GGNNCC 2 cut(s) 533, 740
BspMAI CTGCAG 3 cut(s) 66, 75, 204
BspMI ACCTGC 1 cut(s) 411
BspOI GCTAGC 1 cut(s) 770
BspPI GGATC 1 cut(s) 396
BspTNI GGTCTC 1 cut(s) 455
BsrGI TGTACA 1 cut(s) 441
BssMI GATC 6 cut(s) 277, 285, 388, 640, 832, 1135
Bst4CI ACNGT 5 cut(s) 536, 803, 956, 1289, 1460
Bst6I CTCTTC 4 cut(s) 453, 521, 1096, 1275
BstAPI GCANNNNNTGC 1 cut(s) 70
BstAUI TGTACA 1 cut(s) 441
BstC8I GCNNGC 3 cut(s) 207, 768, 1480
BstDEI CTNAG 4 cut(s) 634, 771, 810, 991
BstENI CCTNNNNNAGG 2 cut(s) 478, 979
BstF5I GGATG 3 cut(s) 59, 91, 1025
BstFNI CGCG 1 cut(s) 149
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 6 cut(s) 280, 288, 391, 643, 835, 1138
BstMAI GTCTC 4 cut(s) 394, 455, 849, 1093
BstMBI GATC 6 cut(s) 277, 285, 388, 640, 832, 1135
BstMCI CGRYCG 1 cut(s) 835
BstMWI GCNNNNNNNGC 4 cut(s) 70, 862, 1040, 1364
BstNSI RCATGY 2 cut(s) 568, 899
BstSFI CTRYAG 3 cut(s) 62, 71, 200
BstSLI GKGCMC 1 cut(s) 1242
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 4 cut(s) 151, 214, 874, 1021
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 2 cut(s) 856, 1112
BtsCI GGATG 3 cut(s) 59, 91, 1025
BtsI GCAGTG 3 cut(s) 149, 191, 1029
BtsIMutI CAGTG 9 cut(s) 149, 191, 295, 808, 961, 1017, 1029, 1237, 1349
BveI ACCTGC 1 cut(s) 411
Cac8I GCNNGC 3 cut(s) 207, 768, 1480
CaiI CAGNNNCTG 1 cut(s) 70
CciI TCATGA 3 cut(s) 339, 616, 1245
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 4 cut(s) 367, 553, 739, 854
Csp6I GTAC 5 cut(s) 442, 782, 1172, 1220, 1290
CviAII CATG 7 cut(s) 340, 559, 565, 617, 896, 1198, 1246
CviQI GTAC 5 cut(s) 442, 782, 1172, 1220, 1290
DdeI CTNAG 4 cut(s) 634, 771, 810, 991
DpnI GATC 6 cut(s) 279, 287, 390, 642, 834, 1137
DpnII GATC 6 cut(s) 277, 285, 388, 640, 832, 1135
DraI TTTAAA 3 cut(s) 129, 604, 1183
DrdI GACNNNNNNGTC 1 cut(s) 469
DseDI GACNNNNNNGTC 1 cut(s) 469
Eam1104I CTCTTC 4 cut(s) 453, 521, 1096, 1275
EarI CTCTTC 4 cut(s) 453, 521, 1096, 1275
EciI GGCGGA 1 cut(s) 194
Ecl136II GAGCTC 1 cut(s) 939
Eco24I GRGCYC 1 cut(s) 941
Eco31I GGTCTC 1 cut(s) 455
Eco47I GGWCC 3 cut(s) 367, 553, 739
Eco53kI GAGCTC 1 cut(s) 939
EcoICRI GAGCTC 1 cut(s) 939
EcoNI CCTNNNNNAGG 2 cut(s) 478, 979
EcoT38I GRGCYC 1 cut(s) 941
FaeI CATG 7 cut(s) 343, 562, 568, 620, 899, 1201, 1249
FalI AAGNNNNNCTT 4 cut(s) 356, 388, 409, 441
FaqI GGGAC 2 cut(s) 752, 1175
FatI CATG 7 cut(s) 339, 558, 564, 616, 895, 1197, 1245
FauNDI CATATG 1 cut(s) 242
FbaI TGATCA 1 cut(s) 277
FblI GTMKAC 1 cut(s) 1414
Fnu4HI GCNGC 4 cut(s) 140, 203, 888, 1035
FokI GGATG 3 cut(s) 66, 98, 1012
FriOI GRGCYC 1 cut(s) 941
Fsp4HI GCNGC 4 cut(s) 140, 203, 888, 1035
FspBI CTAG 4 cut(s) 236, 485, 767, 1044
GlaI GCGC 1 cut(s) 148
GluI GCNGC 4 cut(s) 140, 203, 888, 1035
HaeIII GGCC 2 cut(s) 856, 1112
HhaI GCGC 1 cut(s) 149
Hin1II CATG 7 cut(s) 343, 562, 568, 620, 899, 1201, 1249
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 1 cut(s) 1415
HindII GTYRAC 1 cut(s) 1415
HinfI GANTC 3 cut(s) 920, 1055, 1066
HphI GGTGA 6 cut(s) 269, 929, 935, 1194, 1369, 1453
Hpy166II GTNNAC 2 cut(s) 901, 1415
Hpy188I TCNGA 6 cut(s) 12, 702, 832, 994, 1071, 1140
Hpy188III TCNNGA 7 cut(s) 51, 236, 340, 518, 617, 718, 1246
Hpy8I GTNNAC 2 cut(s) 901, 1415
HpyAV CCTTC 8 cut(s) 358, 769, 817, 907, 1102, 1215, 1263, 1338
HpyCH4III ACNGT 5 cut(s) 536, 803, 956, 1289, 1460
HpyCH4IV ACGT 1 cut(s) 660
HpyCH4V TGCA 7 cut(s) 34, 64, 73, 202, 806, 1037, 1478
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 862, 1040, 1364
HpyF3I CTNAG 4 cut(s) 634, 771, 810, 991
HpySE526I ACGT 1 cut(s) 660
Hsp92II CATG 7 cut(s) 343, 562, 568, 620, 899, 1201, 1249
HspAI GCGC 1 cut(s) 147
Ksp22I TGATCA 1 cut(s) 277
Kzo9I GATC 6 cut(s) 277, 285, 388, 640, 832, 1135
LmnI GCTCC 2 cut(s) 181, 936
Lsp1109I GCAGC 4 cut(s) 151, 214, 874, 1021
LweI GCATC 1 cut(s) 815
MaeI CTAG 4 cut(s) 236, 485, 767, 1044
MaeII ACGT 1 cut(s) 660
MaeIII GTNAC 4 cut(s) 393, 661, 917, 1200
MalI GATC 6 cut(s) 279, 287, 390, 642, 834, 1137
MboI GATC 6 cut(s) 277, 285, 388, 640, 832, 1135
MfeI CAATTG 2 cut(s) 210, 329
MhlI GDGCHC 3 cut(s) 941, 1242, 1390
MlyI GAGTC 2 cut(s) 914, 1064
MseI TTAA 5 cut(s) 128, 603, 645, 860, 1182
MslI CAYNNNNRTG 1 cut(s) 563
MunI CAATTG 2 cut(s) 210, 329
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 4 cut(s) 70, 862, 1040, 1364
NdeI CATATG 1 cut(s) 242
NdeII GATC 6 cut(s) 277, 285, 388, 640, 832, 1135
NheI GCTAGC 1 cut(s) 766
NlaIII CATG 7 cut(s) 343, 562, 568, 620, 899, 1201, 1249
NlaIV GGNNCC 2 cut(s) 533, 740
NmuCI GTSAC 2 cut(s) 917, 1200
NspI RCATGY 2 cut(s) 568, 899
PagI TCATGA 3 cut(s) 339, 616, 1245
PciI ACATGT 1 cut(s) 895
PfeI GAWTC 1 cut(s) 1066
PkrI GCNGC 4 cut(s) 141, 204, 889, 1036
Ple19I CGATCG 1 cut(s) 835
PleI GAGTC 2 cut(s) 914, 1063
PpsI GAGTC 2 cut(s) 914, 1063
PscI ACATGT 1 cut(s) 895
PshBI ATTAAT 1 cut(s) 860
Psp124BI GAGCTC 1 cut(s) 941
PspN4I GGNNCC 2 cut(s) 533, 740
PspPI GGNCC 4 cut(s) 367, 553, 739, 854
PstI CTGCAG 3 cut(s) 66, 75, 204
PstNI CAGNNNCTG 1 cut(s) 70
PvuI CGATCG 1 cut(s) 835
RsaI GTAC 5 cut(s) 443, 783, 1173, 1221, 1291
RsaNI GTAC 5 cut(s) 442, 782, 1172, 1220, 1290
RseI CAYNNNNRTG 1 cut(s) 563
SacI GAGCTC 1 cut(s) 941
SalI GTCGAC 1 cut(s) 1413
SaqAI TTAA 5 cut(s) 128, 603, 645, 860, 1182
SatI GCNGC 4 cut(s) 140, 203, 888, 1035
Sau3AI GATC 6 cut(s) 277, 285, 388, 640, 832, 1135
Sau96I GGNCC 4 cut(s) 367, 553, 739, 854
ScaI AGTACT 1 cut(s) 783
SchI GAGTC 2 cut(s) 914, 1064
SduI GDGCHC 3 cut(s) 941, 1242, 1390
SfaNI GCATC 1 cut(s) 815
SfcI CTRYAG 3 cut(s) 62, 71, 200
SinI GGWCC 3 cut(s) 367, 553, 739
SmiMI CAYNNNNRTG 1 cut(s) 563
SsiI CCGC 2 cut(s) 149, 179
SspMI CTAG 4 cut(s) 236, 485, 767, 1044
SstI GAGCTC 1 cut(s) 941
TaaI ACNGT 5 cut(s) 536, 803, 956, 1289, 1460
TaiI ACGT 1 cut(s) 663
TaqI TCGA 6 cut(s) 407, 678, 835, 1106, 1164, 1414
TaqII GACCGA 1 cut(s) 479
TatI WGTACW 3 cut(s) 441, 781, 1171
TfiI GAWTC 1 cut(s) 1066
Tru1I TTAA 5 cut(s) 128, 603, 645, 860, 1182
Tru9I TTAA 5 cut(s) 128, 603, 645, 860, 1182
TscAI CASTG 9 cut(s) 149, 191, 295, 808, 961, 1017, 1036, 1237, 1356
TseFI GTSAC 2 cut(s) 917, 1200
TseI GCWGC 4 cut(s) 139, 202, 887, 1034
Tsp45I GTSAC 2 cut(s) 917, 1200
TspDTI ATGAA 7 cut(s) 102, 356, 575, 605, 633, 1293, 1334
TspRI CASTG 9 cut(s) 149, 191, 295, 808, 961, 1017, 1036, 1237, 1356
VpaK11BI GGWCC 3 cut(s) 367, 553, 739
VspI ATTAAT 1 cut(s) 860
XagI CCTNNNNNAGG 2 cut(s) 478, 979
XapI RAATTY 2 cut(s) 749, 1123
XbaI TCTAGA 1 cut(s) 235
XceI RCATGY 2 cut(s) 568, 899
XcmI CCANNNNNNNNNTGG 1 cut(s) 953
XmiI GTMKAC 1 cut(s) 1414
XspI CTAG 4 cut(s) 236, 485, 767, 1044
ZrmI AGTACT 1 cut(s) 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.