Rh4DG149100

microtubule binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001938
Physical Location & Seq
Forward (+)
29822980 .. 29823649
670 bp
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UTR
Exon/CDS
Intron
Rh4DG149100.1

Sequence Viewer

Length: 393 bp
ATGGTGAGGAGGGCTCTGATTCTCAATTCCTTTTCTCTGTCTCTCTGGTTGAAAAGGTTATTAAGGGCTGCGTTAAAGATTGGGAAGCTGTTTGCATCGAATGGCGGCCTTGAATATTTGCAGTGCATGGAGGTGTTTAGGACAAAAGTTGACGAGGCGGAACAGTGTAAAGCTCAACTGCAGCAAGCAATTGCTAATTGTGAACCAGAGTTTGTAGACATATGTGCTGTCATGGGTGAGAAACCACCACATTTTGATCATAATATCAGTGGAAGTTTGAAGAAAGAGCTTGAAATCATTATCTCACAATTGGAGGTCATGAAGAAGCGGAAAATTGAAAGGAAGGACCAATTGTTTTCTATCTTGGATCAGTTACAGAGACTTTCGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

130

Amino Acids

14.93

Weight (kDa)

8.96

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 41 - 129 5.9e-12 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 216
AciI CCGC 3 cut(s) 105, 158, 328
AclWI GGATC 1 cut(s) 375
AgsI TTSAA 5 cut(s) 52, 113, 280, 293, 338
AluBI AGCT 3 cut(s) 88, 173, 289
AluI AGCT 3 cut(s) 88, 173, 289
Alw26I GTCTC 2 cut(s) 45, 373
AlwI GGATC 1 cut(s) 375
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 2 cut(s) 68, 181
AspS9I GGNCC 1 cut(s) 346
AsuHPI GGTGA 2 cut(s) 16, 248
AvaII GGWCC 1 cut(s) 346
BanII GRGCYC 1 cut(s) 16
BbvI GCAGC 2 cut(s) 55, 193
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 2 cut(s) 45, 373
BfmI CTRYAG 1 cut(s) 179
BisI GCNGC 3 cut(s) 69, 106, 182
BlsI GCNGC 3 cut(s) 70, 107, 183
Bme18I GGWCC 1 cut(s) 346
BmgT120I GGNCC 1 cut(s) 346
BmsI GCATC 1 cut(s) 104
BplI GAGNNNNNCTC 1 cut(s) 30
BseRI GAGGAG 1 cut(s) 22
BseXI GCAGC 2 cut(s) 55, 193
BshFI GGCC 1 cut(s) 108
BsmAI GTCTC 2 cut(s) 45, 373
BsnI GGCC 1 cut(s) 108
Bsp1286I GDGCHC 1 cut(s) 16
Bsp143I GATC 2 cut(s) 256, 367
BspACI CCGC 3 cut(s) 105, 158, 328
BspANI GGCC 1 cut(s) 108
BspHI TCATGA 1 cut(s) 318
BspMAI CTGCAG 1 cut(s) 183
BspPI GGATC 1 cut(s) 375
BssMI GATC 2 cut(s) 256, 367
Bst4CI ACNGT 1 cut(s) 165
BstC8I GCNNGC 1 cut(s) 186
BstKTI GATC 2 cut(s) 259, 370
BstMAI GTCTC 2 cut(s) 45, 373
BstMBI GATC 2 cut(s) 256, 367
BstSFI CTRYAG 1 cut(s) 179
BstV1I GCAGC 2 cut(s) 55, 193
BsuRI GGCC 1 cut(s) 108
BtsI GCAGTG 1 cut(s) 128
BtsIMutI CAGTG 3 cut(s) 128, 170, 274
Cac8I GCNNGC 1 cut(s) 186
CciI TCATGA 1 cut(s) 318
Cfr13I GGNCC 1 cut(s) 346
CviAII CATG 3 cut(s) 127, 232, 319
CviJI RGCY 6 cut(s) 14, 68, 88, 108, 173, 289
CviKI_1 RGCY 6 cut(s) 14, 68, 88, 108, 173, 289
DpnI GATC 2 cut(s) 258, 369
DpnII GATC 2 cut(s) 256, 367
EciI GGCGGA 1 cut(s) 173
Eco24I GRGCYC 1 cut(s) 16
Eco47I GGWCC 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 16
FaeI CATG 3 cut(s) 130, 235, 322
FaiI YATR 7 cut(s) 128, 221, 223, 233, 261, 320, 391
FatI CATG 3 cut(s) 126, 231, 318
FauNDI CATATG 1 cut(s) 221
FbaI TGATCA 1 cut(s) 256
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 3 cut(s) 69, 106, 182
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 3 cut(s) 69, 106, 182
GluI GCNGC 3 cut(s) 69, 106, 182
HaeIII GGCC 1 cut(s) 108
Hin1II CATG 3 cut(s) 130, 235, 322
HincII GTYRAC 1 cut(s) 151
HindII GTYRAC 1 cut(s) 151
HinfI GANTC 1 cut(s) 19
HphI GGTGA 2 cut(s) 16, 248
Hpy166II GTNNAC 3 cut(s) 151, 203, 217
Hpy188I TCNGA 1 cut(s) 18
Hpy188III TCNNGA 1 cut(s) 319
Hpy8I GTNNAC 3 cut(s) 151, 203, 217
HpyAV CCTTC 1 cut(s) 337
HpyCH4III ACNGT 1 cut(s) 165
HpyCH4V TGCA 4 cut(s) 95, 121, 126, 181
Hsp92II CATG 3 cut(s) 130, 235, 322
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 2 cut(s) 256, 367
LpnPI CCDG 2 cut(s) 31, 219
Lsp1109I GCAGC 2 cut(s) 55, 193
LweI GCATC 1 cut(s) 104
MaeIII GTNAC 1 cut(s) 372
MalI GATC 2 cut(s) 258, 369
MboI GATC 2 cut(s) 256, 367
MboII GAAGA 2 cut(s) 292, 334
MfeI CAATTG 3 cut(s) 189, 308, 350
MhlI GDGCHC 1 cut(s) 16
MluCI AATT 6 cut(s) 25, 189, 196, 308, 333, 350
MnlI CCTC 4 cut(s) 3, 124, 148, 307
MseI TTAA 2 cut(s) 62, 74
MslI CAYNNNNRTG 1 cut(s) 131
MunI CAATTG 3 cut(s) 189, 308, 350
NdeI CATATG 1 cut(s) 221
NdeII GATC 2 cut(s) 256, 367
NlaIII CATG 3 cut(s) 130, 235, 322
PagI TCATGA 1 cut(s) 318
PfeI GAWTC 1 cut(s) 19
PkrI GCNGC 3 cut(s) 70, 107, 183
PspPI GGNCC 1 cut(s) 346
PstI CTGCAG 1 cut(s) 183
RseI CAYNNNNRTG 1 cut(s) 131
SaqAI TTAA 2 cut(s) 62, 74
SatI GCNGC 3 cut(s) 69, 106, 182
Sau3AI GATC 2 cut(s) 256, 367
Sau96I GGNCC 1 cut(s) 346
SduI GDGCHC 1 cut(s) 16
SetI ASST 6 cut(s) 59, 90, 135, 175, 291, 318
SfaNI GCATC 1 cut(s) 104
SfcI CTRYAG 1 cut(s) 179
SinI GGWCC 1 cut(s) 346
SmiMI CAYNNNNRTG 1 cut(s) 131
Sse9I AATT 6 cut(s) 25, 189, 196, 308, 333, 350
SsiI CCGC 3 cut(s) 105, 158, 328
SspI AATATT 1 cut(s) 116
TaaI ACNGT 1 cut(s) 165
TaqI TCGA 2 cut(s) 98, 386
TasI AATT 6 cut(s) 25, 189, 196, 308, 333, 350
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 1 cut(s) 19
Tru1I TTAA 2 cut(s) 62, 74
Tru9I TTAA 2 cut(s) 62, 74
TscAI CASTG 3 cut(s) 128, 170, 274
TseI GCWGC 2 cut(s) 68, 181
TspDTI ATGAA 1 cut(s) 335
TspRI CASTG 3 cut(s) 128, 170, 274
VpaK11BI GGWCC 1 cut(s) 346
XmiI GTMKAC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.