Rroxscaffold_5G00337990

microtubule binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0001938
Physical Location & Seq
Forward (+)
6212631 .. 6216320
3690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00337990.1

Sequence Viewer

Length: 1023 bp
ATGCTTTTCTCGGTCCATTACGGTGGTCCTCTTCGAACTCTAGCTGTAATTGAGGTAAAACTTTCTGCTAATTCATTGATCCATAAGCAATTAGTTGTGGGAGAGTTCAAACATGGAGAGGCCCTTGTGGAAGGCCACAAAAGTCAAATTGTTTGCTCCCCCGAGATATATACATGGAAATATGGTTCTGAACTTCTGATCTGTAAGGAAGAAGACAATATTCTTGTTTACAATACTGAATTGGGTTGCCGTTTGTTTCTTCATTTTGTTCTTCATTTTGCTACTTTCATATATCGTTCATTAGTGGGAAGCATTCCATCTTGGCCGGGCCAAGATAATCTGTATAAAATGGTGGTGGAAGAAACCGACTTGTCCTTGAAAAGGCTAGAAGAACTACATAAGCAGTTGCTTGAATATCAAGATGAGAAGAGGAACCGTTTGAAGTTGATAATGGACCACATGAGCATGCTCAACTCCCTGTGCTTGGTTCTTGGTATGGATTTTAAACACACAATTCATGAAATCCACCCCACCTTAGATGATCTTAATGGAGAAAAAGACGTAACGAATAGCACAATCGAGGGTTTGGCTAATTCAGTTCAAATATTGAGAGAGGTCAAGATACAGAGATGGCAGAGGCTTCAAACTTTCGCAAGTGCCCTCTTGGAGATGTGGAATCTGATGGATACAACCATGGAGGAGCAAACGAAATATCAGAATGTAACTAGTCGCATAGCTGCTACAAAATCTGAAATCACTGAGCCTAACATTCTTTCTGTAGACCTTCTAAATGATGTTGAAGCTGAAGTGTCAAAGTTGGAGCTGCTCAAGTCAATTAAGCTCAAAGAGATCCTTCTGAAAAAGAAGTTGGAACTGGAGGAGGTATGCAGATATTCTCACATGGTTACAGAAATATTGAGTGCTGCAGAATACTCAAATGAAGCTATAGCTTGGGAAAAAGAAAGAGGACTAGAATTCTTGTACGATGGTGTAAAGGGGTTTCAGTCTAGCAAAAGTTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

340

Amino Acids

39.2

Weight (kDa)

5.54

Isoelectric Point (pI)

42.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 118 - 321 4.4e-26 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 780
AclWI GGATC 2 cut(s) 73, 844
AcoI YGGCCR 1 cut(s) 323
AcsI RAATTY 1 cut(s) 974
AcuI CTGAAG 1 cut(s) 825
AfaI GTAC 1 cut(s) 983
AfiI CCNNNNNNNGG 2 cut(s) 381, 484
AgsI TTSAA 7 cut(s) 109, 379, 413, 442, 602, 644, 800
AhlI ACTAGT 1 cut(s) 725
AjuI GAANNNNNNNTTGG 2 cut(s) 851, 883
AloI GAACNNNNNNTCC 2 cut(s) 169, 201
AluBI AGCT 7 cut(s) 44, 737, 803, 823, 841, 944, 950
AluI AGCT 7 cut(s) 44, 737, 803, 823, 841, 944, 950
AlwI GGATC 2 cut(s) 73, 844
Ama87I CYCGRG 1 cut(s) 161
AoxI GGCC 4 cut(s) 120, 133, 323, 328
ApeKI GCWGC 3 cut(s) 737, 823, 923
ApoI RAATTY 1 cut(s) 974
AspS9I GGNCC 5 cut(s) 13, 26, 121, 328, 454
AsuC2I CCSGG 1 cut(s) 327
AsuII TTCGAA 1 cut(s) 34
AvaI CYCGRG 1 cut(s) 161
AvaII GGWCC 3 cut(s) 13, 26, 454
BaeGI GKGCMC 1 cut(s) 661
BbsI GAAGAC 1 cut(s) 219
BbvI GCAGC 3 cut(s) 724, 810, 910
BccI CCATC 4 cut(s) 325, 624, 676, 980
BceAI ACGGC 1 cut(s) 234
BciVI GTATCC 1 cut(s) 679
BcnI CCSGG 1 cut(s) 327
BcuI ACTAGT 1 cut(s) 725
BfaI CTAG 5 cut(s) 41, 386, 726, 971, 1008
BfmI CTRYAG 3 cut(s) 777, 924, 945
BfuI GTATCC 1 cut(s) 679
BisI GCNGC 3 cut(s) 738, 824, 924
BlsI GCNGC 3 cut(s) 739, 825, 925
Bme1390I CCNGG 1 cut(s) 327
Bme18I GGWCC 3 cut(s) 13, 26, 454
BmeT110I CYCGRG 1 cut(s) 161
BmgT120I GGNCC 5 cut(s) 13, 26, 121, 328, 454
BmiI GGNNCC 1 cut(s) 434
BmrFI CCNGG 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 219
BpmI CTGGAG 1 cut(s) 896
Bpu14I TTCGAA 1 cut(s) 34
BpuEI CTTGAG 1 cut(s) 812
BpuMI CCSGG 1 cut(s) 327
BsaJI CCNNGG 1 cut(s) 693
Bsc4I CCNNNNNNNGG 2 cut(s) 381, 484
Bse1I ACTGG 1 cut(s) 879
BseDI CCNNGG 1 cut(s) 693
BseLI CCNNNNNNNGG 2 cut(s) 381, 484
BseMII CTCAG 1 cut(s) 750
BseNI ACTGG 1 cut(s) 879
BseRI GAGGAG 2 cut(s) 713, 893
BseSI GKGCMC 1 cut(s) 661
BseXI GCAGC 3 cut(s) 724, 810, 910
BshFI GGCC 4 cut(s) 122, 135, 325, 330
BsiHKCI CYCGRG 1 cut(s) 161
BsiSI CCGG 1 cut(s) 326
BslI CCNNNNNNNGG 2 cut(s) 381, 484
BsmI GAATGC 1 cut(s) 312
BsnI GGCC 4 cut(s) 122, 135, 325, 330
BsoBI CYCGRG 1 cut(s) 161
Bsp119I TTCGAA 1 cut(s) 34
Bsp1286I GDGCHC 1 cut(s) 661
Bsp143I GATC 4 cut(s) 78, 198, 541, 849
Bsp19I CCATGG 1 cut(s) 693
BspANI GGCC 4 cut(s) 122, 135, 325, 330
BspCNI CTCAG 1 cut(s) 751
BspHI TCATGA 1 cut(s) 517
BspLI GGNNCC 1 cut(s) 434
BspMAI CTGCAG 1 cut(s) 928
BspPI GGATC 2 cut(s) 73, 844
BspT104I TTCGAA 1 cut(s) 34
BsrI ACTGG 1 cut(s) 879
BssECI CCNNGG 1 cut(s) 693
BssMI GATC 4 cut(s) 78, 198, 541, 849
BssT1I CCWWGG 1 cut(s) 693
Bst4CI ACNGT 2 cut(s) 23, 437
Bst6I CTCTTC 2 cut(s) 36, 422
BstBI TTCGAA 1 cut(s) 34
BstC8I GCNNGC 1 cut(s) 467
BstDEI CTNAG 2 cut(s) 535, 759
BstDSI CCRYGG 1 cut(s) 693
BstENI CCTNNNNNAGG 1 cut(s) 379
BstKTI GATC 4 cut(s) 81, 201, 544, 852
BstMBI GATC 4 cut(s) 78, 198, 541, 849
BstNSI RCATGY 1 cut(s) 469
BstSCI CCNGG 1 cut(s) 325
BstSFI CTRYAG 3 cut(s) 777, 924, 945
BstSLI GKGCMC 1 cut(s) 661
BstV1I GCAGC 3 cut(s) 724, 810, 910
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 1 cut(s) 849
BstXI CCANNNNNNTGG 1 cut(s) 23
BstYI RGATCY 1 cut(s) 849
BsuI GTATCC 1 cut(s) 679
BsuRI GGCC 4 cut(s) 122, 135, 325, 330
BtgI CCRYGG 1 cut(s) 693
BtsIMutI CAGTG 1 cut(s) 756
Cac8I GCNNGC 1 cut(s) 467
CciI TCATGA 1 cut(s) 517
Cfr13I GGNCC 5 cut(s) 13, 26, 121, 328, 454
Csp6I GTAC 1 cut(s) 982
CviAII CATG 7 cut(s) 113, 174, 460, 466, 518, 694, 901
CviQI GTAC 1 cut(s) 982
DdeI CTNAG 2 cut(s) 535, 759
DpnI GATC 4 cut(s) 80, 200, 543, 851
DpnII GATC 4 cut(s) 78, 198, 541, 849
DraI TTTAAA 1 cut(s) 505
EaeI YGGCCR 1 cut(s) 323
Eam1104I CTCTTC 2 cut(s) 36, 422
EarI CTCTTC 2 cut(s) 36, 422
Eco130I CCWWGG 1 cut(s) 693
Eco47I GGWCC 3 cut(s) 13, 26, 454
Eco57I CTGAAG 1 cut(s) 825
Eco88I CYCGRG 1 cut(s) 161
EcoNI CCTNNNNNAGG 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 121
EcoRI GAATTC 1 cut(s) 974
EcoT14I CCWWGG 1 cut(s) 693
ErhI CCWWGG 1 cut(s) 693
FaeI CATG 7 cut(s) 116, 177, 463, 469, 521, 697, 904
FalI AAGNNNNNCTT 2 cut(s) 837, 869
FatI CATG 7 cut(s) 112, 173, 459, 465, 517, 693, 900
FblI GTMKAC 1 cut(s) 780
Fnu4HI GCNGC 3 cut(s) 738, 824, 924
Fsp4HI GCNGC 3 cut(s) 738, 824, 924
FspBI CTAG 5 cut(s) 41, 386, 726, 971, 1008
GluI GCNGC 3 cut(s) 738, 824, 924
GsuI CTGGAG 1 cut(s) 896
HaeIII GGCC 4 cut(s) 122, 135, 325, 330
HapII CCGG 1 cut(s) 326
Hin1II CATG 7 cut(s) 116, 177, 463, 469, 521, 697, 904
HinfI GANTC 1 cut(s) 676
HpaII CCGG 1 cut(s) 326
Hpy166II GTNNAC 2 cut(s) 229, 781
Hpy188I TCNGA 6 cut(s) 190, 198, 681, 717, 751, 858
Hpy188III TCNNGA 3 cut(s) 419, 518, 619
Hpy8I GTNNAC 2 cut(s) 229, 781
HpyAV CCTTC 3 cut(s) 125, 794, 863
HpyCH4III ACNGT 2 cut(s) 23, 437
HpyCH4IV ACGT 1 cut(s) 561
HpyCH4V TGCA 2 cut(s) 888, 926
HpyF3I CTNAG 2 cut(s) 535, 759
HpySE526I ACGT 1 cut(s) 561
Hsp92II CATG 7 cut(s) 116, 177, 463, 469, 521, 697, 904
Kzo9I GATC 4 cut(s) 78, 198, 541, 849
LmnI GCTCC 3 cut(s) 161, 700, 820
LpnPI CCDG 3 cut(s) 339, 491, 860
Lsp1109I GCAGC 3 cut(s) 724, 810, 910
MaeI CTAG 5 cut(s) 41, 386, 726, 971, 1008
MaeII ACGT 1 cut(s) 561
MaeIII GTNAC 3 cut(s) 562, 721, 904
MalI GATC 4 cut(s) 80, 200, 543, 851
MboI GATC 4 cut(s) 78, 198, 541, 849
MboII GAAGA 8 cut(s) 23, 221, 224, 251, 263, 371, 401, 439
MflI RGATCY 1 cut(s) 849
MhlI GDGCHC 1 cut(s) 661
MluCI AATT 9 cut(s) 48, 70, 89, 147, 239, 513, 592, 834, 974
MmeI TCCRAC 2 cut(s) 798, 849
MseI TTAA 3 cut(s) 504, 546, 837
MslI CAYNNNNRTG 2 cut(s) 21, 464
MspI CCGG 1 cut(s) 326
MspR9I CCNGG 1 cut(s) 327
Mva1269I GAATGC 1 cut(s) 312
NciI CCSGG 1 cut(s) 327
NcoI CCATGG 1 cut(s) 693
NdeII GATC 4 cut(s) 78, 198, 541, 849
NlaIII CATG 7 cut(s) 116, 177, 463, 469, 521, 697, 904
NlaIV GGNNCC 1 cut(s) 434
NspI RCATGY 1 cut(s) 469
NspV TTCGAA 1 cut(s) 34
PaeI GCATGC 1 cut(s) 469
PagI TCATGA 1 cut(s) 517
PctI GAATGC 1 cut(s) 312
PfeI GAWTC 1 cut(s) 676
PkrI GCNGC 3 cut(s) 739, 825, 925
PspN4I GGNNCC 1 cut(s) 434
PspPI GGNCC 5 cut(s) 13, 26, 121, 328, 454
PstI CTGCAG 1 cut(s) 928
PsuI RGATCY 1 cut(s) 849
RsaI GTAC 1 cut(s) 983
RsaNI GTAC 1 cut(s) 982
RseI CAYNNNNRTG 2 cut(s) 21, 464
SaqAI TTAA 3 cut(s) 504, 546, 837
SatI GCNGC 3 cut(s) 738, 824, 924
Sau3AI GATC 4 cut(s) 78, 198, 541, 849
Sau96I GGNCC 5 cut(s) 13, 26, 121, 328, 454
ScrFI CCNGG 1 cut(s) 327
SduI GDGCHC 1 cut(s) 661
SfcI CTRYAG 3 cut(s) 777, 924, 945
SfuI TTCGAA 1 cut(s) 34
SinI GGWCC 3 cut(s) 13, 26, 454
SmiMI CAYNNNNRTG 2 cut(s) 21, 464
SmlI CTYRAG 1 cut(s) 827
SmoI CTYRAG 1 cut(s) 827
SpeI ACTAGT 1 cut(s) 725
SphI GCATGC 1 cut(s) 469
Sse9I AATT 9 cut(s) 48, 70, 89, 147, 239, 513, 592, 834, 974
SspI AATATT 3 cut(s) 220, 606, 915
SspMI CTAG 5 cut(s) 41, 386, 726, 971, 1008
StyD4I CCNGG 1 cut(s) 325
StyI CCWWGG 1 cut(s) 693
TaaI ACNGT 2 cut(s) 23, 437
TaiI ACGT 1 cut(s) 564
TaqI TCGA 2 cut(s) 34, 579
TasI AATT 9 cut(s) 48, 70, 89, 147, 239, 513, 592, 834, 974
TfiI GAWTC 1 cut(s) 676
Tru1I TTAA 3 cut(s) 504, 546, 837
Tru9I TTAA 3 cut(s) 504, 546, 837
TscAI CASTG 1 cut(s) 763
TseI GCWGC 3 cut(s) 737, 823, 923
TspDTI ATGAA 8 cut(s) 63, 251, 263, 277, 288, 506, 534, 954
TspRI CASTG 1 cut(s) 763
VpaK11BI GGWCC 3 cut(s) 13, 26, 454
XagI CCTNNNNNAGG 1 cut(s) 379
XapI RAATTY 1 cut(s) 974
XceI RCATGY 1 cut(s) 469
XmiI GTMKAC 1 cut(s) 780
XspI CTAG 5 cut(s) 41, 386, 726, 971, 1008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.