Rroxscaffold_4G00320860

microtubule binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001938
Physical Location & Seq
Forward (+)
49853807 .. 49865340
11534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00320860.1

Sequence Viewer

Length: 912 bp
ATGGTGGTGGAAGAGACCGACTTGTCCTTGAAAAGGCTACAAGAACTACATAAGCAGTTGCTTGAATATCAAGATGAGAAGAGGAACCGTTTGAAGCTGATAATGGACCACATGAGCATGCTAAACTCCCTGTGCTTGGTTCTTGGTATGGATTTTAAACACACAATTTATGAAATCCACCCTACCTTAGATGATCTTAATGGAGAAAAAGACGTAACGAATAGCACAATCGAGGGTTTGGCTAATTCGGTTCAAATATTGAGAGAGGTCAAGATACAGAGATGGCAGAGGCTTCAAACTTTCGCAAGTGCCCTCTTGGAGATGTGGAATCTGATGGATACAACCATGGAGGAGCAAACGAAATATCAGAATGTAACTAGTCGCATAGCTGCTACAGAATCTGAAATCACTGAGCCTAACATTCTTTCTGTAGACCTTCTAAATGATATTGAAGCTGAAGTGTCAAAGTTGGAGCTGCTCAAGTCAATTAAGCTCAAAGAGATCCTTCTGAAAAAGAAGTTGGAACTGGAGGAGGTATGCAGACAGTCTCACATGGTTACAGAAATACTGAGTGCTGCAGAATACTCAAATGAAGCTATAGCTTGGGAAAAAGAAAGAGGACTAGAATTCTTGTACGATGGTGTAAGTACTTTCAAAGTTAGACCAATTGGTAAAAGTTATTCAAATTGTGGTTTAGGGTTGGTCATATTCACATGGGCCCACTCCGATTGGCGGGCACTCTCTTTCGCCGCACAGAGCAGCAGGGGCCTTGCTCCCTCCAGCAGCCCTTTGCTCGTTGCCACTCTAGTCCCGGCTCCACGGTCGGCCGGCGGGGTTGAGATGCCAATGGTGTGGCATTGGCATCACCACGTGTGTGGGATCGGCTCACCGCCGGTGTCCGCGGATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

303

Amino Acids

34.36

Weight (kDa)

5.44

Isoelectric Point (pI)

49.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 4 - 205 2.3e-26 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 22
AccI GTMKAC 1 cut(s) 432
AccII CGCG 1 cut(s) 902
AciI CCGC 6 cut(s) 733, 750, 831, 890, 900, 902
AclWI GGATC 2 cut(s) 496, 887
AcoI YGGCCR 1 cut(s) 825
AcsI RAATTY 1 cut(s) 626
AcuI CTGAAG 1 cut(s) 477
AcvI CACGTG 1 cut(s) 871
AdeI CACNNNGTG 1 cut(s) 871
AfaI GTAC 2 cut(s) 635, 649
AfiI CCNNNNNNNGG 3 cut(s) 33, 136, 732
AflIII ACRYGT 1 cut(s) 870
AgsI TTSAA 8 cut(s) 31, 65, 94, 254, 296, 452, 655, 684
AhlI ACTAGT 1 cut(s) 377
AjuI GAANNNNNNNTTGG 2 cut(s) 503, 535
AleI CACNNNNGTG 1 cut(s) 873
AluBI AGCT 7 cut(s) 97, 389, 455, 475, 493, 596, 602
AluI AGCT 7 cut(s) 97, 389, 455, 475, 493, 596, 602
Alw26I GTCTC 2 cut(s) 8, 552
AlwI GGATC 2 cut(s) 496, 887
AlwNI CAGNNNCTG 1 cut(s) 401
AoxI GGCC 3 cut(s) 717, 766, 825
ApaI GGGCCC 1 cut(s) 721
ApeKI GCWGC 5 cut(s) 389, 475, 575, 759, 783
ApoI RAATTY 1 cut(s) 626
AspS9I GGNCC 4 cut(s) 106, 717, 718, 766
AsuC2I CCSGG 1 cut(s) 812
AsuHPI GGTGA 2 cut(s) 857, 879
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 3 cut(s) 313, 721, 739
BanII GRGCYC 1 cut(s) 721
BbrPI CACGTG 1 cut(s) 871
BbvI GCAGC 5 cut(s) 376, 462, 562, 771, 795
BccI CCATC 3 cut(s) 276, 328, 632
BciVI GTATCC 1 cut(s) 331
BcnI CCSGG 1 cut(s) 812
BcoDI GTCTC 2 cut(s) 8, 552
BcuI ACTAGT 1 cut(s) 377
BfaI CTAG 3 cut(s) 378, 623, 806
BfmI CTRYAG 4 cut(s) 393, 429, 576, 597
BfuI GTATCC 1 cut(s) 331
BisI GCNGC 6 cut(s) 390, 476, 576, 750, 760, 784
BlsI GCNGC 6 cut(s) 391, 477, 577, 751, 761, 785
BmcAI AGTACT 1 cut(s) 649
Bme1390I CCNGG 1 cut(s) 812
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 4 cut(s) 106, 717, 718, 766
BmiI GGNNCC 4 cut(s) 86, 719, 767, 816
BmrFI CCNGG 1 cut(s) 812
BmsI GCATC 2 cut(s) 831, 871
BpmI CTGGAG 2 cut(s) 548, 763
BpuEI CTTGAG 1 cut(s) 464
BpuMI CCSGG 1 cut(s) 812
BsaAI YACGTR 1 cut(s) 871
BsaI GGTCTC 1 cut(s) 8
BsaJI CCNNGG 3 cut(s) 345, 818, 900
Bsc4I CCNNNNNNNGG 3 cut(s) 33, 136, 732
Bse118I RCCGGY 2 cut(s) 827, 892
Bse1I ACTGG 1 cut(s) 531
BseDI CCNNGG 3 cut(s) 345, 818, 900
BseLI CCNNNNNNNGG 3 cut(s) 33, 136, 732
BseMII CTCAG 2 cut(s) 402, 560
BseNI ACTGG 1 cut(s) 531
BseRI GAGGAG 2 cut(s) 365, 545
BseSI GKGCMC 3 cut(s) 313, 721, 739
BseX3I CGGCCG 1 cut(s) 825
BseXI GCAGC 5 cut(s) 376, 462, 562, 771, 795
Bsh1236I CGCG 1 cut(s) 902
Bsh1285I CGRYCG 2 cut(s) 824, 828
BshFI GGCC 3 cut(s) 719, 768, 827
BsiEI CGRYCG 2 cut(s) 824, 828
BsiSI CCGG 3 cut(s) 812, 828, 893
BslFI GGGAC 1 cut(s) 794
BslI CCNNNNNNNGG 3 cut(s) 33, 136, 732
BsmAI GTCTC 2 cut(s) 8, 552
BsmFI GGGAC 1 cut(s) 794
BsnI GGCC 3 cut(s) 719, 768, 827
Bso31I GGTCTC 1 cut(s) 8
Bsp120I GGGCCC 1 cut(s) 717
Bsp1286I GDGCHC 3 cut(s) 313, 721, 739
Bsp143I GATC 4 cut(s) 193, 501, 879, 904
Bsp19I CCATGG 1 cut(s) 345
BspACI CCGC 6 cut(s) 733, 750, 831, 890, 900, 902
BspANI GGCC 3 cut(s) 719, 768, 827
BspCNI CTCAG 2 cut(s) 403, 561
BspFNI CGCG 1 cut(s) 902
BspLI GGNNCC 4 cut(s) 86, 719, 767, 816
BspMAI CTGCAG 1 cut(s) 580
BspPI GGATC 2 cut(s) 496, 887
BspTNI GGTCTC 1 cut(s) 8
BsrFI RCCGGY 2 cut(s) 827, 892
BsrI ACTGG 1 cut(s) 531
BssAI RCCGGY 2 cut(s) 827, 892
BssECI CCNNGG 3 cut(s) 345, 818, 900
BssMI GATC 4 cut(s) 193, 501, 879, 904
BssT1I CCWWGG 1 cut(s) 345
Bst4CI ACNGT 3 cut(s) 89, 546, 822
Bst6I CTCTTC 2 cut(s) 6, 74
BstBAI YACGTR 1 cut(s) 871
BstC8I GCNNGC 3 cut(s) 119, 735, 829
BstDEI CTNAG 3 cut(s) 187, 411, 569
BstDSI CCRYGG 3 cut(s) 345, 818, 900
BstENI CCTNNNNNAGG 1 cut(s) 31
BstFNI CGCG 1 cut(s) 902
BstKTI GATC 4 cut(s) 196, 504, 882, 907
BstMAI GTCTC 2 cut(s) 8, 552
BstMBI GATC 4 cut(s) 193, 501, 879, 904
BstMCI CGRYCG 2 cut(s) 824, 828
BstMWI GCNNNNNNNGC 1 cut(s) 765
BstNSI RCATGY 1 cut(s) 121
BstSCI CCNGG 1 cut(s) 810
BstSFI CTRYAG 4 cut(s) 393, 429, 576, 597
BstSLI GKGCMC 3 cut(s) 313, 721, 739
BstUI CGCG 1 cut(s) 902
BstV1I GCAGC 5 cut(s) 376, 462, 562, 771, 795
BstX2I RGATCY 1 cut(s) 501
BstXI CCANNNNNNTGG 2 cut(s) 852, 875
BstYI RGATCY 1 cut(s) 501
BstZI CGGCCG 1 cut(s) 825
BsuI GTATCC 1 cut(s) 331
BsuRI GGCC 3 cut(s) 719, 768, 827
BtgI CCRYGG 3 cut(s) 345, 818, 900
BtsIMutI CAGTG 1 cut(s) 408
Cac8I GCNNGC 3 cut(s) 119, 735, 829
CaiI CAGNNNCTG 1 cut(s) 401
Cfr10I RCCGGY 2 cut(s) 827, 892
Cfr13I GGNCC 4 cut(s) 106, 717, 718, 766
Cfr42I CCGCGG 1 cut(s) 903
Csp6I GTAC 2 cut(s) 634, 648
CspCI CAANNNNNGTGG 2 cut(s) 710, 745
CviAII CATG 5 cut(s) 112, 118, 346, 553, 714
CviQI GTAC 2 cut(s) 634, 648
DdeI CTNAG 3 cut(s) 187, 411, 569
DpnI GATC 4 cut(s) 195, 503, 881, 906
DpnII GATC 4 cut(s) 193, 501, 879, 904
DraI TTTAAA 1 cut(s) 157
DraIII CACNNNGTG 1 cut(s) 871
DrdI GACNNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 825
EagI CGGCCG 1 cut(s) 825
Eam1104I CTCTTC 2 cut(s) 6, 74
EarI CTCTTC 2 cut(s) 6, 74
EclXI CGGCCG 1 cut(s) 825
Eco130I CCWWGG 1 cut(s) 345
Eco24I GRGCYC 1 cut(s) 721
Eco31I GGTCTC 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 106
Eco52I CGGCCG 1 cut(s) 825
Eco57I CTGAAG 1 cut(s) 477
Eco72I CACGTG 1 cut(s) 871
EcoNI CCTNNNNNAGG 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 766
EcoRI GAATTC 1 cut(s) 626
EcoT14I CCWWGG 1 cut(s) 345
EcoT38I GRGCYC 1 cut(s) 721
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 5 cut(s) 115, 121, 349, 556, 717
FalI AAGNNNNNCTT 2 cut(s) 489, 521
FaqI GGGAC 1 cut(s) 794
FatI CATG 5 cut(s) 111, 117, 345, 552, 713
FauI CCCGC 2 cut(s) 726, 824
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 6 cut(s) 390, 476, 576, 750, 760, 784
FriOI GRGCYC 1 cut(s) 721
Fsp4HI GCNGC 6 cut(s) 390, 476, 576, 750, 760, 784
FspBI CTAG 3 cut(s) 378, 623, 806
GluI GCNGC 6 cut(s) 390, 476, 576, 750, 760, 784
GsuI CTGGAG 2 cut(s) 548, 763
HaeIII GGCC 3 cut(s) 719, 768, 827
HapII CCGG 3 cut(s) 812, 828, 893
Hin1II CATG 5 cut(s) 115, 121, 349, 556, 717
HinfI GANTC 2 cut(s) 328, 398
HpaII CCGG 3 cut(s) 812, 828, 893
HphI GGTGA 2 cut(s) 857, 879
Hpy166II GTNNAC 1 cut(s) 433
Hpy188I TCNGA 5 cut(s) 333, 369, 403, 510, 727
Hpy188III TCNNGA 2 cut(s) 71, 271
Hpy8I GTNNAC 1 cut(s) 433
HpyAV CCTTC 2 cut(s) 446, 515
HpyCH4III ACNGT 3 cut(s) 89, 546, 822
HpyCH4IV ACGT 2 cut(s) 213, 870
HpyCH4V TGCA 2 cut(s) 540, 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 765
HpyF3I CTNAG 3 cut(s) 187, 411, 569
HpySE526I ACGT 2 cut(s) 213, 870
Hsp92II CATG 5 cut(s) 115, 121, 349, 556, 717
KroI GCCGGC 1 cut(s) 827
KroNI GCCGGC 1 cut(s) 829
KspI CCGCGG 1 cut(s) 903
Kzo9I GATC 4 cut(s) 193, 501, 879, 904
LmnI GCTCC 4 cut(s) 352, 472, 778, 820
LpnPI CCDG 7 cut(s) 143, 512, 748, 793, 825, 841, 906
Lsp1109I GCAGC 5 cut(s) 376, 462, 562, 771, 795
LweI GCATC 2 cut(s) 831, 871
MaeI CTAG 3 cut(s) 378, 623, 806
MaeII ACGT 2 cut(s) 213, 870
MaeIII GTNAC 3 cut(s) 214, 373, 556
MalI GATC 4 cut(s) 195, 503, 881, 906
MboI GATC 4 cut(s) 193, 501, 879, 904
MboII GAAGA 2 cut(s) 23, 91
MfeI CAATTG 1 cut(s) 666
MflI RGATCY 1 cut(s) 501
MhlI GDGCHC 3 cut(s) 313, 721, 739
MluCI AATT 6 cut(s) 165, 244, 486, 626, 666, 685
MmeI TCCRAC 2 cut(s) 450, 501
MroNI GCCGGC 1 cut(s) 827
MseI TTAA 3 cut(s) 156, 198, 489
MslI CAYNNNNRTG 2 cut(s) 116, 873
MspA1I CMGCKG 1 cut(s) 902
MspI CCGG 3 cut(s) 812, 828, 893
MspR9I CCNGG 1 cut(s) 812
MunI CAATTG 1 cut(s) 666
MvnI CGCG 1 cut(s) 902
MwoI GCNNNNNNNGC 1 cut(s) 765
NaeI GCCGGC 1 cut(s) 829
NciI CCSGG 1 cut(s) 812
NcoI CCATGG 1 cut(s) 345
NdeII GATC 4 cut(s) 193, 501, 879, 904
NgoMIV GCCGGC 1 cut(s) 827
NlaIII CATG 5 cut(s) 115, 121, 349, 556, 717
NlaIV GGNNCC 4 cut(s) 86, 719, 767, 816
NspI RCATGY 1 cut(s) 121
OliI CACNNNNGTG 1 cut(s) 873
PaeI GCATGC 1 cut(s) 121
PdiI GCCGGC 1 cut(s) 829
PfeI GAWTC 2 cut(s) 328, 398
PkrI GCNGC 6 cut(s) 391, 477, 577, 751, 761, 785
PmaCI CACGTG 1 cut(s) 871
PmlI CACGTG 1 cut(s) 871
Ppu21I YACGTR 1 cut(s) 871
PspCI CACGTG 1 cut(s) 871
PspN4I GGNNCC 4 cut(s) 86, 719, 767, 816
PspOMI GGGCCC 1 cut(s) 717
PspPI GGNCC 4 cut(s) 106, 717, 718, 766
PstI CTGCAG 1 cut(s) 580
PstNI CAGNNNCTG 1 cut(s) 401
PsuI RGATCY 1 cut(s) 501
RsaI GTAC 2 cut(s) 635, 649
RsaNI GTAC 2 cut(s) 634, 648
RseI CAYNNNNRTG 2 cut(s) 116, 873
SacII CCGCGG 1 cut(s) 903
SaqAI TTAA 3 cut(s) 156, 198, 489
SatI GCNGC 6 cut(s) 390, 476, 576, 750, 760, 784
Sau3AI GATC 4 cut(s) 193, 501, 879, 904
Sau96I GGNCC 4 cut(s) 106, 717, 718, 766
ScaI AGTACT 1 cut(s) 649
ScrFI CCNGG 1 cut(s) 812
SduI GDGCHC 3 cut(s) 313, 721, 739
SfaNI GCATC 2 cut(s) 831, 871
SfcI CTRYAG 4 cut(s) 393, 429, 576, 597
Sfr303I CCGCGG 1 cut(s) 903
SgrAI CRCCGGYG 1 cut(s) 892
SgrBI CCGCGG 1 cut(s) 903
SinI GGWCC 1 cut(s) 106
SmiMI CAYNNNNRTG 2 cut(s) 116, 873
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SpeI ACTAGT 1 cut(s) 377
SphI GCATGC 1 cut(s) 121
Sse9I AATT 6 cut(s) 165, 244, 486, 626, 666, 685
SsiI CCGC 6 cut(s) 733, 750, 831, 890, 900, 902
SspI AATATT 1 cut(s) 258
SspMI CTAG 3 cut(s) 378, 623, 806
StyD4I CCNGG 1 cut(s) 810
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 3 cut(s) 89, 546, 822
TaiI ACGT 2 cut(s) 216, 873
TaqI TCGA 1 cut(s) 231
TaqII GACCGA 1 cut(s) 32
TasI AATT 6 cut(s) 165, 244, 486, 626, 666, 685
TatI WGTACW 1 cut(s) 647
TauI GCSGC 1 cut(s) 752
TfiI GAWTC 2 cut(s) 328, 398
Tru1I TTAA 3 cut(s) 156, 198, 489
Tru9I TTAA 3 cut(s) 156, 198, 489
TscAI CASTG 1 cut(s) 415
TseI GCWGC 5 cut(s) 389, 475, 575, 759, 783
TspDTI ATGAA 2 cut(s) 186, 606
TspRI CASTG 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 106
XagI CCTNNNNNAGG 1 cut(s) 31
XapI RAATTY 1 cut(s) 626
XceI RCATGY 1 cut(s) 121
XmiI GTMKAC 1 cut(s) 432
XspI CTAG 3 cut(s) 378, 623, 806
ZrmI AGTACT 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.