Rw6G014400

microtubule binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001938
Physical Location & Seq
Reverse (-)
26654715 .. 26660958
6244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G014400.1

Sequence Viewer

Length: 1230 bp
ATGGAGGTGTTTAGGACAAAAGTTGACGAGGCGGAACAGTGTAAAGCTCAACTGCAGCAAGCAATTGCTAATTGTGAACCAGAGTTTGTAGACATATGTGCTGTCATGGGTGAGAAACCACCACATTTTGATCATAATATCAGTGGAAGTTTGAAGAAAGAGCTTGAAATCATTATCCCACAATTGGAGGTCATGAAGAAGCGGAAAATTGAAAGGAAGGACCAATTGTTTTCTATCTTGGATCAGTCCTTGGAAGATAATCTGTACAAAATGGTGGTGGAAGAGACCGACTTGTCCTTGAAAAGGCTAGAAGAACTACATAGGCAGTTGCTTGAATATCAAGATGAGAAGAGGAACCGTTTGAAGCTGATAATGGACCACATGAGCATGCTAAACTCCCTGTGCTTGGTTCTTGGTATGGATTTTAAACACACAATTCATGAAATCCACCCCACCTTAGATGATCTTAATGGAGAAAAAGACGTAACGAACAGCACAATCGAGGGTTTGGCTAATTCGGTTCAGATATTGAGAGAGGTCAAGATACAGAGATGGCAGAGGCTTCAAACTTTCGCAAGTGCCCTCTTAGAGATGTGGAATCTGATGGATACAACCATGGAGGAGCAAAAGAAATATCAGAATGTAACTAGTCGCATAGCTGCTTCAGAATCTGAAATCACTGAGCCTAACATTCTTTCTGTAGACCTTCTAAATGATAAGTTGGAACTGGAGGAGGTATGCAGACAGTATCACATGGTTACAGAAATACTGAGTGCTGCAAAATACTCAAATGAAGCTATAGAGTCTGGAGCTGTGGACCCTGCATGCCTGTTGGAACAAATTGAGCATCAAATCGCAATTGATATTTTGTACACTTGTTCCTTTCTCTTCTCCCAGGTATTTATTTCCTCTATCTATAATATCTACTTTTCACTCTTCAATGATCATTTTGAACATGGTTTTGTGAAAAAAGGAGTTAAAATGAGATACTTATGCTATTTTGAACAAATGAAATTGATCGTACAACAGTTTTTAGTGTGGCAATCCAACCACACAAGTATGGGACTCCAACACAACATGCACAAAAGCTATCCAAAGTTGGAAGAATATTATGACCAATCTTCCAAAAAAAAAAAAGATAGGAAGAGAACGACAATCAACAACCCTGCAAAGCACAAGCAATCAAACAATACATCTATAGAAAAAAGATTTTTATTTATTTATTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

409

Amino Acids

48.03

Weight (kDa)

5.8

Isoelectric Point (pI)

53.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 2 - 285 2.2e-28 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 292
AccI GTMKAC 2 cut(s) 90, 702
AciI CCGC 2 cut(s) 32, 202
AclWI GGATC 1 cut(s) 249
AcuI CTGAAG 1 cut(s) 648
AfaI GTAC 3 cut(s) 266, 872, 1023
AfiI CCNNNNNNNGG 3 cut(s) 184, 303, 406
AhlI ACTAGT 1 cut(s) 647
AjnI CCWGG 1 cut(s) 894
AluBI AGCT 7 cut(s) 47, 163, 367, 659, 797, 812, 1089
AluI AGCT 7 cut(s) 47, 163, 367, 659, 797, 812, 1089
Alw26I GTCTC 1 cut(s) 278
AlwI GGATC 1 cut(s) 249
AlwNI CAGNNNCTG 1 cut(s) 671
ApeKI GCWGC 3 cut(s) 55, 659, 776
AspS9I GGNCC 3 cut(s) 220, 376, 817
AsuHPI GGTGA 1 cut(s) 122
AvaII GGWCC 3 cut(s) 220, 376, 817
BaeGI GKGCMC 1 cut(s) 583
BbvI GCAGC 3 cut(s) 67, 646, 763
BccI CCATC 2 cut(s) 546, 598
BciT130I CCWGG 1 cut(s) 896
BciVI GTATCC 1 cut(s) 601
BclI TGATCA 2 cut(s) 130, 943
BcoDI GTCTC 1 cut(s) 278
BcuI ACTAGT 1 cut(s) 647
BfaI CTAG 2 cut(s) 308, 648
BfmI CTRYAG 4 cut(s) 53, 699, 798, 1197
BfuI GTATCC 1 cut(s) 601
BisI GCNGC 3 cut(s) 56, 660, 777
BlsI GCNGC 3 cut(s) 57, 661, 778
Bme1390I CCNGG 1 cut(s) 896
Bme18I GGWCC 3 cut(s) 220, 376, 817
BmgT120I GGNCC 3 cut(s) 220, 376, 817
BmiI GGNNCC 2 cut(s) 356, 819
BmrFI CCNGG 1 cut(s) 896
BmsI GCATC 1 cut(s) 856
BpmI CTGGAG 2 cut(s) 749, 828
BsaI GGTCTC 1 cut(s) 278
BsaJI CCNNGG 3 cut(s) 249, 615, 894
Bsc4I CCNNNNNNNGG 3 cut(s) 184, 303, 406
Bse1I ACTGG 1 cut(s) 732
BseBI CCWGG 1 cut(s) 896
BseDI CCNNGG 3 cut(s) 249, 615, 894
BseLI CCNNNNNNNGG 3 cut(s) 184, 303, 406
BseMII CTCAG 2 cut(s) 672, 761
BseNI ACTGG 1 cut(s) 732
BseRI GAGGAG 2 cut(s) 635, 746
BseSI GKGCMC 1 cut(s) 583
BseXI GCAGC 3 cut(s) 67, 646, 763
BslFI GGGAC 1 cut(s) 1077
BslI CCNNNNNNNGG 3 cut(s) 184, 303, 406
BsmAI GTCTC 1 cut(s) 278
BsmFI GGGAC 1 cut(s) 1077
Bso31I GGTCTC 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 583
Bsp1407I TGTACA 2 cut(s) 264, 870
Bsp143I GATC 5 cut(s) 130, 241, 463, 943, 1017
Bsp19I CCATGG 1 cut(s) 615
BspACI CCGC 2 cut(s) 32, 202
BspCNI CTCAG 2 cut(s) 673, 762
BspHI TCATGA 2 cut(s) 192, 439
BspLI GGNNCC 2 cut(s) 356, 819
BspMAI CTGCAG 1 cut(s) 57
BspPI GGATC 1 cut(s) 249
BspTNI GGTCTC 1 cut(s) 278
BsrGI TGTACA 2 cut(s) 264, 870
BsrI ACTGG 1 cut(s) 732
BssECI CCNNGG 3 cut(s) 249, 615, 894
BssMI GATC 5 cut(s) 130, 241, 463, 943, 1017
BssT1I CCWWGG 2 cut(s) 249, 615
Bst2UI CCWGG 1 cut(s) 896
Bst4CI ACNGT 4 cut(s) 39, 359, 747, 1029
Bst6I CTCTTC 5 cut(s) 276, 344, 893, 941, 1139
BstAUI TGTACA 2 cut(s) 264, 870
BstC8I GCNNGC 3 cut(s) 60, 389, 826
BstDEI CTNAG 4 cut(s) 457, 586, 681, 770
BstDSI CCRYGG 1 cut(s) 615
BstENI CCTNNNNNAGG 1 cut(s) 301
BstKTI GATC 5 cut(s) 133, 244, 466, 946, 1020
BstMAI GTCTC 1 cut(s) 278
BstMBI GATC 5 cut(s) 130, 241, 463, 943, 1017
BstNI CCWGG 1 cut(s) 896
BstNSI RCATGY 3 cut(s) 391, 828, 1081
BstSCI CCNGG 1 cut(s) 894
BstSFI CTRYAG 4 cut(s) 53, 699, 798, 1197
BstSLI GKGCMC 1 cut(s) 583
BstV1I GCAGC 3 cut(s) 67, 646, 763
BsuI GTATCC 1 cut(s) 601
BtgI CCRYGG 1 cut(s) 615
BtsIMutI CAGTG 3 cut(s) 44, 148, 678
Cac8I GCNNGC 3 cut(s) 60, 389, 826
CaiI CAGNNNCTG 1 cut(s) 671
CciI TCATGA 2 cut(s) 192, 439
Cfr13I GGNCC 3 cut(s) 220, 376, 817
Csp6I GTAC 3 cut(s) 265, 871, 1022
CviQI GTAC 3 cut(s) 265, 871, 1022
DdeI CTNAG 4 cut(s) 457, 586, 681, 770
DpnI GATC 5 cut(s) 132, 243, 465, 945, 1019
DpnII GATC 5 cut(s) 130, 241, 463, 943, 1017
DraI TTTAAA 1 cut(s) 427
DrdI GACNNNNNNGTC 1 cut(s) 292
DseDI GACNNNNNNGTC 1 cut(s) 292
Eam1104I CTCTTC 5 cut(s) 276, 344, 893, 941, 1139
EarI CTCTTC 5 cut(s) 276, 344, 893, 941, 1139
EciI GGCGGA 1 cut(s) 47
Eco130I CCWWGG 2 cut(s) 249, 615
Eco31I GGTCTC 1 cut(s) 278
Eco47I GGWCC 3 cut(s) 220, 376, 817
Eco57I CTGAAG 1 cut(s) 648
EcoNI CCTNNNNNAGG 1 cut(s) 301
EcoRII CCWGG 1 cut(s) 894
EcoT14I CCWWGG 2 cut(s) 249, 615
ErhI CCWWGG 2 cut(s) 249, 615
FaqI GGGAC 1 cut(s) 1077
FauNDI CATATG 1 cut(s) 95
FbaI TGATCA 2 cut(s) 130, 943
FblI GTMKAC 2 cut(s) 90, 702
Fnu4HI GCNGC 3 cut(s) 56, 660, 777
Fsp4HI GCNGC 3 cut(s) 56, 660, 777
FspBI CTAG 2 cut(s) 308, 648
GluI GCNGC 3 cut(s) 56, 660, 777
GsuI CTGGAG 2 cut(s) 749, 828
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HinfI GANTC 4 cut(s) 598, 668, 803, 1065
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 6 cut(s) 25, 77, 91, 703, 817, 873
Hpy188I TCNGA 5 cut(s) 525, 603, 639, 667, 673
Hpy188III TCNNGA 5 cut(s) 193, 341, 440, 541, 807
Hpy8I GTNNAC 6 cut(s) 25, 77, 91, 703, 817, 873
HpyAV CCTTC 2 cut(s) 211, 716
HpyCH4III ACNGT 4 cut(s) 39, 359, 747, 1029
HpyCH4IV ACGT 1 cut(s) 483
HpyCH4V TGCA 6 cut(s) 55, 741, 779, 824, 1081, 1169
HpyF3I CTNAG 4 cut(s) 457, 586, 681, 770
HpySE526I ACGT 1 cut(s) 483
Ksp22I TGATCA 2 cut(s) 130, 943
Kzo9I GATC 5 cut(s) 130, 241, 463, 943, 1017
LmnI GCTCC 2 cut(s) 622, 809
LpnPI CCDG 9 cut(s) 93, 413, 713, 792, 834, 842, 881, 908, 1179
Lsp1109I GCAGC 3 cut(s) 67, 646, 763
LweI GCATC 1 cut(s) 856
MaeI CTAG 2 cut(s) 308, 648
MaeII ACGT 1 cut(s) 483
MaeIII GTNAC 3 cut(s) 484, 643, 757
MalI GATC 5 cut(s) 132, 243, 465, 945, 1019
MboI GATC 5 cut(s) 130, 241, 463, 943, 1017
MfeI CAATTG 4 cut(s) 63, 182, 224, 858
MhlI GDGCHC 1 cut(s) 583
MlyI GAGTC 2 cut(s) 812, 1059
MmeI TCCRAC 5 cut(s) 702, 813, 1071, 1080, 1093
MseI TTAA 3 cut(s) 426, 468, 978
MslI CAYNNNNRTG 2 cut(s) 386, 1058
MspR9I CCNGG 1 cut(s) 896
MunI CAATTG 4 cut(s) 63, 182, 224, 858
MvaI CCWGG 1 cut(s) 896
NcoI CCATGG 1 cut(s) 615
NdeI CATATG 1 cut(s) 95
NdeII GATC 5 cut(s) 130, 241, 463, 943, 1017
NlaIV GGNNCC 2 cut(s) 356, 819
NspI RCATGY 3 cut(s) 391, 828, 1081
PaeI GCATGC 2 cut(s) 391, 828
PagI TCATGA 2 cut(s) 192, 439
PfeI GAWTC 2 cut(s) 598, 668
PkrI GCNGC 3 cut(s) 57, 661, 778
PleI GAGTC 2 cut(s) 811, 1059
PpsI GAGTC 2 cut(s) 811, 1059
Psp6I CCWGG 1 cut(s) 894
PspGI CCWGG 1 cut(s) 894
PspN4I GGNNCC 2 cut(s) 356, 819
PspPI GGNCC 3 cut(s) 220, 376, 817
PstI CTGCAG 1 cut(s) 57
PstNI CAGNNNCTG 1 cut(s) 671
RsaI GTAC 3 cut(s) 266, 872, 1023
RsaNI GTAC 3 cut(s) 265, 871, 1022
RseI CAYNNNNRTG 2 cut(s) 386, 1058
SaqAI TTAA 3 cut(s) 426, 468, 978
SatI GCNGC 3 cut(s) 56, 660, 777
Sau3AI GATC 5 cut(s) 130, 241, 463, 943, 1017
Sau96I GGNCC 3 cut(s) 220, 376, 817
SchI GAGTC 2 cut(s) 812, 1059
ScrFI CCNGG 1 cut(s) 896
SduI GDGCHC 1 cut(s) 583
SfaNI GCATC 1 cut(s) 856
SfcI CTRYAG 4 cut(s) 53, 699, 798, 1197
SinI GGWCC 3 cut(s) 220, 376, 817
SmiMI CAYNNNNRTG 2 cut(s) 386, 1058
SpeI ACTAGT 1 cut(s) 647
SphI GCATGC 2 cut(s) 391, 828
SsiI CCGC 2 cut(s) 32, 202
SspI AATATT 1 cut(s) 1109
SspMI CTAG 2 cut(s) 308, 648
StyD4I CCNGG 1 cut(s) 894
StyI CCWWGG 2 cut(s) 249, 615
TaaI ACNGT 4 cut(s) 39, 359, 747, 1029
TaiI ACGT 1 cut(s) 486
TaqI TCGA 1 cut(s) 501
TaqII GACCGA 1 cut(s) 302
TatI WGTACW 2 cut(s) 264, 870
TfiI GAWTC 2 cut(s) 598, 668
Tru1I TTAA 3 cut(s) 426, 468, 978
Tru9I TTAA 3 cut(s) 426, 468, 978
TscAI CASTG 3 cut(s) 44, 148, 685
TseI GCWGC 3 cut(s) 55, 659, 776
TspDTI ATGAA 5 cut(s) 209, 428, 456, 807, 1025
TspRI CASTG 3 cut(s) 44, 148, 685
VpaK11BI GGWCC 3 cut(s) 220, 376, 817
XagI CCTNNNNNAGG 1 cut(s) 301
XceI RCATGY 3 cut(s) 391, 828, 1081
XmiI GTMKAC 2 cut(s) 90, 702
XspI CTAG 2 cut(s) 308, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.