RLG00000028307

Microtubule associated protein (MAP65/ASE1 family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001938
Physical Location & Seq
Forward (+)
21293256 .. 21295693
2438 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028307

Sequence Viewer

Length: 927 bp
ATGGTGGTGGAAGAGACCGACTTGTCATTGAAAAGGCTAGAAGAACTACATAGGCAGTTGCTTGAATATCAAGATGAGAAGAGTAACCGTTTGAGGCTGATAATGGACCACATGAGCATGCTAAACTCCCTGTGCTTGGTTCTTGGCATGGATTTTAAACACACAATTCATGAAATCCACCCCACCTTAGTTGATCTTAATGGAGAAAAAGACGTAACGAACAGCACAATCGAGGGTTTGGCTAATTCACTTCAAACTTTCGCAAGTGCCCTCTTGGAGATGTGGAATCTGATGGATACAACCATGGAGGAGCAAAAGAAATATCAGAATGTAACTAGTCGCATAGCTACTTCAGAATCTGAAATCACTAAGCTTAAAATTCTTTCTGTAGACATTCTAAATGATGTTGAAGCTGAAGTGTCAAAGTTGGACCAGCTCAAGTCAATTAAGCTCAAAGAGATCCTTCTGAAAAAGAAGTTGGAACTGGAGGAGGTATGCAGACAGTCTCACATGGTTACAGAAATATCGAGTGCTGCAGAATACTCAAATGAAGGTATAGAGTCTGGAGCTGCATGCCTGTTGGAACAAATTGAGCTTCAAATCGCAAGGGCAAAAGAGGAAGCTCTAAGCAGGAAAGAAATACTAGAAAAGATTGAAAAGTGGTTGGCTGCTTGTCAAGAAGAATCCTGGCTAGAGAAGTTAATAGGGTTGAGAATTCTATTCGATGATAATCGCTATACAGCAGGAAGAGGTGCTCATATTACTTTGAAACGTGCAAAGAAAGTGCTTGTTTTAGTAAACAAAATTCCTGGAATGGCGGAAACATTGACTTCAAAAGCCACAGCTTGGGAAAAAGAAAGAGGACTAGAATTCTTGTACGATGGTACTTTCAAAGTTAGACCAATTGGTGAAGGGGTTTCAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

309

Amino Acids

35.06

Weight (kDa)

5.32

Isoelectric Point (pI)

44.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 4 - 296 1.1e-48 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 22
AccB7I CCANNNNNTGG 1 cut(s) 846
AccI GTMKAC 1 cut(s) 390
AciI CCGC 1 cut(s) 818
AclWI GGATC 1 cut(s) 454
AcsI RAATTY 4 cut(s) 378, 714, 804, 869
AcuI CTGAAG 2 cut(s) 336, 435
AfaI GTAC 2 cut(s) 878, 886
AfiI CCNNNNNNNGG 2 cut(s) 136, 846
AgsI TTSAA 9 cut(s) 31, 65, 254, 410, 599, 656, 769, 834, 892
AhlI ACTAGT 1 cut(s) 335
AjnI CCWGG 2 cut(s) 686, 808
AjuI GAANNNNNNNTTGG 2 cut(s) 461, 493
AluBI AGCT 9 cut(s) 347, 373, 413, 436, 451, 569, 595, 623, 845
AluI AGCT 9 cut(s) 347, 373, 413, 436, 451, 569, 595, 623, 845
Alw21I GWGCWC 1 cut(s) 757
Alw26I GTCTC 2 cut(s) 8, 510
AlwI GGATC 1 cut(s) 454
AlwNI CAGNNNCTG 1 cut(s) 359
ApeKI GCWGC 3 cut(s) 533, 569, 668
ApoI RAATTY 4 cut(s) 378, 714, 804, 869
AspS9I GGNCC 2 cut(s) 106, 430
AsuHPI GGTGA 1 cut(s) 920
AvaII GGWCC 2 cut(s) 106, 430
BaeGI GKGCMC 1 cut(s) 271
Bbv12I GWGCWC 1 cut(s) 757
BbvI GCAGC 3 cut(s) 520, 556, 655
BccI CCATC 2 cut(s) 286, 875
BciT130I CCWGG 2 cut(s) 688, 810
BciVI GTATCC 1 cut(s) 289
BcoDI GTCTC 2 cut(s) 8, 510
BcuI ACTAGT 1 cut(s) 335
BfaI CTAG 6 cut(s) 38, 336, 644, 692, 866, 925
BfmI CTRYAG 2 cut(s) 387, 534
BfuI GTATCC 1 cut(s) 289
BisI GCNGC 3 cut(s) 534, 570, 669
BlsI GCNGC 3 cut(s) 535, 571, 670
Bme1390I CCNGG 2 cut(s) 688, 810
Bme18I GGWCC 2 cut(s) 106, 430
BmgT120I GGNCC 2 cut(s) 106, 430
BmrFI CCNGG 2 cut(s) 688, 810
BpmI CTGGAG 2 cut(s) 506, 585
BpuEI CTTGAG 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 729
BsaI GGTCTC 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 303
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 846
Bse1I ACTGG 1 cut(s) 489
Bse8I GATNNNNATC 1 cut(s) 729
BseBI CCWGG 2 cut(s) 688, 810
BseDI CCNNGG 1 cut(s) 303
BseJI GATNNNNATC 1 cut(s) 729
BseLI CCNNNNNNNGG 2 cut(s) 136, 846
BseNI ACTGG 1 cut(s) 489
BseRI GAGGAG 2 cut(s) 323, 503
BseSI GKGCMC 1 cut(s) 271
BseXI GCAGC 3 cut(s) 520, 556, 655
BsiHKAI GWGCWC 1 cut(s) 757
BslI CCNNNNNNNGG 2 cut(s) 136, 846
BsmAI GTCTC 2 cut(s) 8, 510
Bso31I GGTCTC 1 cut(s) 8
Bsp1286I GDGCHC 2 cut(s) 271, 757
Bsp143I GATC 2 cut(s) 193, 459
Bsp19I CCATGG 1 cut(s) 303
BspACI CCGC 1 cut(s) 818
BspHI TCATGA 1 cut(s) 169
BspMAI CTGCAG 1 cut(s) 538
BspPI GGATC 1 cut(s) 454
BspTNI GGTCTC 1 cut(s) 8
BsrI ACTGG 1 cut(s) 489
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 2 cut(s) 193, 459
BssT1I CCWWGG 1 cut(s) 303
Bst2UI CCWGG 2 cut(s) 688, 810
Bst4CI ACNGT 2 cut(s) 89, 504
Bst6I CTCTTC 3 cut(s) 6, 74, 742
BstC8I GCNNGC 2 cut(s) 119, 574
BstDEI CTNAG 3 cut(s) 187, 369, 626
BstDSI CCRYGG 1 cut(s) 303
BstKTI GATC 2 cut(s) 196, 462
BstMAI GTCTC 2 cut(s) 8, 510
BstMBI GATC 2 cut(s) 193, 459
BstNI CCWGG 2 cut(s) 688, 810
BstNSI RCATGY 2 cut(s) 121, 576
BstSCI CCNGG 2 cut(s) 686, 808
BstSFI CTRYAG 2 cut(s) 387, 534
BstSLI GKGCMC 1 cut(s) 271
BstV1I GCAGC 3 cut(s) 520, 556, 655
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
BsuI GTATCC 1 cut(s) 289
BtgI CCRYGG 1 cut(s) 303
Cac8I GCNNGC 2 cut(s) 119, 574
CaiI CAGNNNCTG 1 cut(s) 359
CciI TCATGA 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 106, 430
Csp6I GTAC 2 cut(s) 877, 885
CspCI CAANNNNNGTGG 2 cut(s) 172, 207
CviAII CATG 7 cut(s) 112, 118, 148, 170, 304, 511, 573
CviQI GTAC 2 cut(s) 877, 885
DdeI CTNAG 3 cut(s) 187, 369, 626
DpnI GATC 2 cut(s) 195, 461
DpnII GATC 2 cut(s) 193, 459
DraI TTTAAA 1 cut(s) 157
DrdI GACNNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 1 cut(s) 22
Eam1104I CTCTTC 3 cut(s) 6, 74, 742
EarI CTCTTC 3 cut(s) 6, 74, 742
EciI GGCGGA 1 cut(s) 833
Eco130I CCWWGG 1 cut(s) 303
Eco31I GGTCTC 1 cut(s) 8
Eco47I GGWCC 2 cut(s) 106, 430
Eco57I CTGAAG 2 cut(s) 336, 435
EcoRI GAATTC 2 cut(s) 714, 869
EcoRII CCWGG 2 cut(s) 686, 808
EcoT14I CCWWGG 1 cut(s) 303
ErhI CCWWGG 1 cut(s) 303
FaeI CATG 7 cut(s) 115, 121, 151, 173, 307, 514, 576
FalI AAGNNNNNCTT 2 cut(s) 447, 479
FatI CATG 7 cut(s) 111, 117, 147, 169, 303, 510, 572
FblI GTMKAC 1 cut(s) 390
Fnu4HI GCNGC 3 cut(s) 534, 570, 669
Fsp4HI GCNGC 3 cut(s) 534, 570, 669
FspBI CTAG 6 cut(s) 38, 336, 644, 692, 866, 925
GluI GCNGC 3 cut(s) 534, 570, 669
GsuI CTGGAG 2 cut(s) 506, 585
Hin1II CATG 7 cut(s) 115, 121, 151, 173, 307, 514, 576
HindIII AAGCTT 1 cut(s) 371
HinfI GANTC 4 cut(s) 286, 356, 560, 683
HphI GGTGA 1 cut(s) 920
Hpy166II GTNNAC 2 cut(s) 391, 799
Hpy188I TCNGA 5 cut(s) 291, 327, 355, 361, 468
Hpy188III TCNNGA 4 cut(s) 71, 170, 564, 677
Hpy8I GTNNAC 2 cut(s) 391, 799
HpyAV CCTTC 3 cut(s) 473, 545, 905
HpyCH4III ACNGT 2 cut(s) 89, 504
HpyCH4IV ACGT 2 cut(s) 213, 772
HpyCH4V TGCA 4 cut(s) 498, 536, 572, 776
HpyF3I CTNAG 3 cut(s) 187, 369, 626
HpySE526I ACGT 2 cut(s) 213, 772
Hsp92II CATG 7 cut(s) 115, 121, 151, 173, 307, 514, 576
Kzo9I GATC 2 cut(s) 193, 459
LmnI GCTCC 2 cut(s) 310, 566
Lsp1109I GCAGC 3 cut(s) 520, 556, 655
MaeI CTAG 6 cut(s) 38, 336, 644, 692, 866, 925
MaeII ACGT 2 cut(s) 213, 772
MaeIII GTNAC 4 cut(s) 83, 214, 331, 514
MalI GATC 2 cut(s) 195, 461
MboI GATC 2 cut(s) 193, 459
MboII GAAGA 5 cut(s) 23, 53, 91, 692, 759
MfeI CAATTG 1 cut(s) 903
MflI RGATCY 1 cut(s) 459
MhlI GDGCHC 2 cut(s) 271, 757
MluCI AATT 9 cut(s) 165, 244, 378, 444, 588, 714, 804, 869, 903
MlyI GAGTC 1 cut(s) 569
MmeI TCCRAC 3 cut(s) 408, 459, 561
MnlI CCTC 9 cut(s) 87, 226, 281, 301, 481, 484, 610, 743, 854
MseI TTAA 5 cut(s) 156, 198, 375, 447, 701
MslI CAYNNNNRTG 1 cut(s) 116
MspR9I CCNGG 2 cut(s) 688, 810
MunI CAATTG 1 cut(s) 903
MvaI CCWGG 2 cut(s) 688, 810
NcoI CCATGG 1 cut(s) 303
NdeII GATC 2 cut(s) 193, 459
NlaIII CATG 7 cut(s) 115, 121, 151, 173, 307, 514, 576
NspI RCATGY 2 cut(s) 121, 576
PaeI GCATGC 2 cut(s) 121, 576
PagI TCATGA 1 cut(s) 169
PfeI GAWTC 3 cut(s) 286, 356, 683
PflMI CCANNNNNTGG 1 cut(s) 846
PfoI TCCNGGA 1 cut(s) 808
PkrI GCNGC 3 cut(s) 535, 571, 670
PleI GAGTC 1 cut(s) 568
PpsI GAGTC 1 cut(s) 568
Psp6I CCWGG 2 cut(s) 686, 808
PspGI CCWGG 2 cut(s) 686, 808
PspPI GGNCC 2 cut(s) 106, 430
PstI CTGCAG 1 cut(s) 538
PstNI CAGNNNCTG 1 cut(s) 359
PsuI RGATCY 1 cut(s) 459
RsaI GTAC 2 cut(s) 878, 886
RsaNI GTAC 2 cut(s) 877, 885
RseI CAYNNNNRTG 1 cut(s) 116
SaqAI TTAA 5 cut(s) 156, 198, 375, 447, 701
SatI GCNGC 3 cut(s) 534, 570, 669
Sau3AI GATC 2 cut(s) 193, 459
Sau96I GGNCC 2 cut(s) 106, 430
SchI GAGTC 1 cut(s) 569
ScrFI CCNGG 2 cut(s) 688, 810
SduI GDGCHC 2 cut(s) 271, 757
SfcI CTRYAG 2 cut(s) 387, 534
SinI GGWCC 2 cut(s) 106, 430
SmiMI CAYNNNNRTG 1 cut(s) 116
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
SpeI ACTAGT 1 cut(s) 335
SphI GCATGC 2 cut(s) 121, 576
Sse9I AATT 9 cut(s) 165, 244, 378, 444, 588, 714, 804, 869, 903
SsiI CCGC 1 cut(s) 818
SspMI CTAG 6 cut(s) 38, 336, 644, 692, 866, 925
StyD4I CCNGG 2 cut(s) 686, 808
StyI CCWWGG 1 cut(s) 303
TaaI ACNGT 2 cut(s) 89, 504
TaiI ACGT 2 cut(s) 216, 775
TaqI TCGA 3 cut(s) 231, 527, 723
TaqII GACCGA 1 cut(s) 32
TasI AATT 9 cut(s) 165, 244, 378, 444, 588, 714, 804, 869, 903
TfiI GAWTC 3 cut(s) 286, 356, 683
Tru1I TTAA 5 cut(s) 156, 198, 375, 447, 701
Tru9I TTAA 5 cut(s) 156, 198, 375, 447, 701
TseI GCWGC 3 cut(s) 533, 569, 668
TspDTI ATGAA 3 cut(s) 158, 186, 564
Van91I CCANNNNNTGG 1 cut(s) 846
VpaK11BI GGWCC 2 cut(s) 106, 430
XapI RAATTY 4 cut(s) 378, 714, 804, 869
XceI RCATGY 2 cut(s) 121, 576
XmiI GTMKAC 1 cut(s) 390
XspI CTAG 6 cut(s) 38, 336, 644, 692, 866, 925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.