Rh4AG144000

microtubule binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001938
Physical Location & Seq
Forward (+)
36451193 .. 36461990
10798 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG144000.1

Sequence Viewer

Length: 777 bp
ATGGCGGCCTTGAATATTTGCAGTGAGTGGAGGTGTTTAGGGCAAAAGTTGACGAGGCGGAACAGTGTAAAGCTGAACTACAGCAAGCAATTGCTAATTGCTGTCCTGGGTGAGAAACCACCACATTTTGATCATAAGATCAGTGGAAGTTTGAAGAAAGAGCTTGAAATCATTATCCCACAATTCGAGGTCATGAAGAAGCGGAAAATTGAAAGGAAGGACCAATTGTTTTCTGTCTTGGATCAGTTACAGAGACTTTCGACAGAAATAAGCAGGTCCTTGGAAGATAATCTGTACAAAATGGTGGTGGAAGCAGAGACCGACTTATCCTTGAAAAGGCTAGAAGAACTACACAGGCAGTTGCTTGAATATCAAGATGAGAAGAGGAACCGTTTGAAGCTTATAATGGACCACATGAGCATGCTAAACTCCCTGTGCTTTGTTCTTGGTATGGATTTTAAACACACAATTCATGAAATCCACCCCACCTTAGATGATTTTAATGGAGAAAAAGACGTAACGAACAGCACAATCGAGGGTTTGGCTAATTCGGTTCAGATATTGAGAGAGGTCAAGATACAGAGATGGCAGAGGTGGCTGAAGAACACCCAGAGTGAAAATGGGATTTTACACGGCGGTTCCATGTCTCTTACACGAGATCTAATTCAGAAAAAGACTGAATTTATGGGGAAGATCGTCTTGGCTACAGAAAATCCTGGTGGGTTCATAAATGGCAAGTGGAAGGCAACTGGCCCAGTTATCTCTACAAGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAP65_ASE1

Protein Analysis

258

Amino Acids

29.85

Weight (kDa)

9.25

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 42 - 198 3.3e-15 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 404
Acc36I ACCTGC 1 cut(s) 264
AciI CCGC 4 cut(s) 5, 58, 202, 636
AclWI GGATC 1 cut(s) 249
AcsI RAATTY 1 cut(s) 680
AcuI CTGAAG 1 cut(s) 620
AfaI GTAC 1 cut(s) 296
AfiI CCNNNNNNNGG 1 cut(s) 336
AgsI TTSAA 7 cut(s) 13, 154, 167, 212, 334, 368, 397
AjnI CCWGG 2 cut(s) 105, 715
AjuI GAANNNNNNNTTGG 2 cut(s) 683, 715
AluBI AGCT 4 cut(s) 73, 163, 400, 773
AluI AGCT 4 cut(s) 73, 163, 400, 773
Alw26I GTCTC 3 cut(s) 247, 311, 651
AlwI GGATC 1 cut(s) 249
AoxI GGCC 2 cut(s) 6, 751
ApoI RAATTY 1 cut(s) 680
AspS9I GGNCC 4 cut(s) 220, 276, 409, 752
AsuHPI GGTGA 1 cut(s) 122
AvaII GGWCC 3 cut(s) 220, 276, 409
BauI CACGAG 1 cut(s) 654
BccI CCATC 1 cut(s) 579
BceAI ACGGC 1 cut(s) 649
BciT130I CCWGG 2 cut(s) 107, 717
BclI TGATCA 1 cut(s) 130
BcoDI GTCTC 3 cut(s) 247, 311, 651
BfaI CTAG 1 cut(s) 341
BfmI CTRYAG 2 cut(s) 79, 705
BfuAI ACCTGC 1 cut(s) 264
BglII AGATCT 1 cut(s) 658
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 107, 717
Bme18I GGWCC 3 cut(s) 220, 276, 409
BmgT120I GGNCC 4 cut(s) 220, 276, 409, 752
BmiI GGNNCC 2 cut(s) 389, 640
BmrFI CCNGG 2 cut(s) 107, 717
BmrI ACTGGG 1 cut(s) 749
BmuI ACTGGG 1 cut(s) 749
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 106, 279
Bsc4I CCNNNNNNNGG 1 cut(s) 336
Bse1I ACTGG 2 cut(s) 754, 755
BseBI CCWGG 2 cut(s) 107, 717
BseDI CCNNGG 2 cut(s) 106, 279
BseLI CCNNNNNNNGG 1 cut(s) 336
BseNI ACTGG 2 cut(s) 754, 755
BshFI GGCC 2 cut(s) 8, 753
BslI CCNNNNNNNGG 1 cut(s) 336
BsmAI GTCTC 3 cut(s) 247, 311, 651
BsnI GGCC 2 cut(s) 8, 753
Bso31I GGTCTC 1 cut(s) 311
Bsp1407I TGTACA 1 cut(s) 294
Bsp143I GATC 5 cut(s) 130, 138, 241, 658, 693
BspACI CCGC 4 cut(s) 5, 58, 202, 636
BspANI GGCC 2 cut(s) 8, 753
BspHI TCATGA 2 cut(s) 192, 472
BspLI GGNNCC 2 cut(s) 389, 640
BspMI ACCTGC 1 cut(s) 264
BspPI GGATC 1 cut(s) 249
BspTNI GGTCTC 1 cut(s) 311
BsrGI TGTACA 1 cut(s) 294
BsrI ACTGG 2 cut(s) 754, 755
BssECI CCNNGG 2 cut(s) 106, 279
BssMI GATC 5 cut(s) 130, 138, 241, 658, 693
BssSI CACGAG 1 cut(s) 654
BssT1I CCWWGG 1 cut(s) 279
Bst2BI CACGAG 1 cut(s) 654
Bst2UI CCWGG 2 cut(s) 107, 717
Bst4CI ACNGT 2 cut(s) 65, 392
Bst6I CTCTTC 1 cut(s) 377
BstAUI TGTACA 1 cut(s) 294
BstC8I GCNNGC 2 cut(s) 86, 422
BstDEI CTNAG 1 cut(s) 490
BstENI CCTNNNNNAGG 1 cut(s) 334
BstKTI GATC 5 cut(s) 133, 141, 244, 661, 696
BstMAI GTCTC 3 cut(s) 247, 311, 651
BstMBI GATC 5 cut(s) 130, 138, 241, 658, 693
BstMWI GCNNNNNNNGC 1 cut(s) 595
BstNI CCWGG 2 cut(s) 107, 717
BstNSI RCATGY 1 cut(s) 424
BstSCI CCNGG 2 cut(s) 105, 715
BstSFI CTRYAG 2 cut(s) 79, 705
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
BsuRI GGCC 2 cut(s) 8, 753
BtsI GCAGTG 1 cut(s) 28
BtsIMutI CAGTG 3 cut(s) 28, 70, 148
BveI ACCTGC 1 cut(s) 264
Cac8I GCNNGC 2 cut(s) 86, 422
CciI TCATGA 2 cut(s) 192, 472
Cfr13I GGNCC 4 cut(s) 220, 276, 409, 752
Csp6I GTAC 1 cut(s) 295
CviAII CATG 5 cut(s) 193, 415, 421, 473, 643
CviQI GTAC 1 cut(s) 295
DdeI CTNAG 1 cut(s) 490
DpnI GATC 5 cut(s) 132, 140, 243, 660, 695
DpnII GATC 5 cut(s) 130, 138, 241, 658, 693
DraI TTTAAA 1 cut(s) 460
Eam1104I CTCTTC 1 cut(s) 377
EarI CTCTTC 1 cut(s) 377
EciI GGCGGA 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 279
Eco31I GGTCTC 1 cut(s) 311
Eco47I GGWCC 3 cut(s) 220, 276, 409
Eco57I CTGAAG 1 cut(s) 620
EcoNI CCTNNNNNAGG 1 cut(s) 334
EcoO109I RGGNCCY 1 cut(s) 276
EcoRII CCWGG 2 cut(s) 105, 715
EcoT14I CCWWGG 1 cut(s) 279
ErhI CCWWGG 1 cut(s) 279
FaeI CATG 5 cut(s) 196, 418, 424, 476, 646
FalI AAGNNNNNCTT 2 cut(s) 683, 715
FatI CATG 5 cut(s) 192, 414, 420, 472, 642
FbaI TGATCA 1 cut(s) 130
Fnu4HI GCNGC 1 cut(s) 6
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 1 cut(s) 341
GluI GCNGC 1 cut(s) 6
HaeIII GGCC 2 cut(s) 8, 753
Hin1II CATG 5 cut(s) 196, 418, 424, 476, 646
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HindIII AAGCTT 1 cut(s) 398
HphI GGTGA 1 cut(s) 122
Hpy166II GTNNAC 1 cut(s) 51
Hpy188I TCNGA 2 cut(s) 558, 669
Hpy188III TCNNGA 4 cut(s) 193, 374, 473, 574
Hpy8I GTNNAC 1 cut(s) 51
HpyAV CCTTC 2 cut(s) 211, 736
HpyCH4III ACNGT 2 cut(s) 65, 392
HpyCH4IV ACGT 1 cut(s) 516
HpyCH4V TGCA 1 cut(s) 21
HpyF10VI GCNNNNNNNGC 1 cut(s) 595
HpyF3I CTNAG 1 cut(s) 490
HpySE526I ACGT 1 cut(s) 516
Hsp92II CATG 5 cut(s) 196, 418, 424, 476, 646
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 5 cut(s) 130, 138, 241, 658, 693
MaeI CTAG 1 cut(s) 341
MaeII ACGT 1 cut(s) 516
MaeIII GTNAC 2 cut(s) 246, 517
MalI GATC 5 cut(s) 132, 140, 243, 660, 695
MboI GATC 5 cut(s) 130, 138, 241, 658, 693
MboII GAAGA 7 cut(s) 166, 208, 296, 356, 394, 613, 703
MfeI CAATTG 2 cut(s) 89, 224
MflI RGATCY 1 cut(s) 658
MluCI AATT 9 cut(s) 89, 96, 182, 207, 224, 468, 547, 663, 680
MnlI CCTC 7 cut(s) 24, 48, 181, 378, 529, 562, 585
MseI TTAA 2 cut(s) 459, 501
MslI CAYNNNNRTG 1 cut(s) 419
MspR9I CCNGG 2 cut(s) 107, 717
MunI CAATTG 2 cut(s) 89, 224
MvaI CCWGG 2 cut(s) 107, 717
MwoI GCNNNNNNNGC 1 cut(s) 595
NdeII GATC 5 cut(s) 130, 138, 241, 658, 693
NlaIII CATG 5 cut(s) 196, 418, 424, 476, 646
NlaIV GGNNCC 2 cut(s) 389, 640
NspI RCATGY 1 cut(s) 424
PaeI GCATGC 1 cut(s) 424
PagI TCATGA 2 cut(s) 192, 472
PkrI GCNGC 1 cut(s) 7
PpuMI RGGWCCY 1 cut(s) 276
PsiI TTATAA 1 cut(s) 404
Psp5II RGGWCCY 1 cut(s) 276
Psp6I CCWGG 2 cut(s) 105, 715
PspGI CCWGG 2 cut(s) 105, 715
PspN4I GGNNCC 2 cut(s) 389, 640
PspPI GGNCC 4 cut(s) 220, 276, 409, 752
PspPPI RGGWCCY 1 cut(s) 276
PsuI RGATCY 1 cut(s) 658
RsaI GTAC 1 cut(s) 296
RsaNI GTAC 1 cut(s) 295
RseI CAYNNNNRTG 1 cut(s) 419
SaqAI TTAA 2 cut(s) 459, 501
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 5 cut(s) 130, 138, 241, 658, 693
Sau96I GGNCC 4 cut(s) 220, 276, 409, 752
ScrFI CCNGG 2 cut(s) 107, 717
SfcI CTRYAG 2 cut(s) 79, 705
SinI GGWCC 3 cut(s) 220, 276, 409
SmiMI CAYNNNNRTG 1 cut(s) 419
SphI GCATGC 1 cut(s) 424
Sse9I AATT 9 cut(s) 89, 96, 182, 207, 224, 468, 547, 663, 680
SsiI CCGC 4 cut(s) 5, 58, 202, 636
SspI AATATT 1 cut(s) 16
SspMI CTAG 1 cut(s) 341
StyD4I CCNGG 2 cut(s) 105, 715
StyI CCWWGG 1 cut(s) 279
TaaI ACNGT 2 cut(s) 65, 392
TaiI ACGT 1 cut(s) 519
TaqI TCGA 3 cut(s) 186, 260, 534
TaqII GACCGA 1 cut(s) 335
TasI AATT 9 cut(s) 89, 96, 182, 207, 224, 468, 547, 663, 680
TatI WGTACW 1 cut(s) 294
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 2 cut(s) 459, 501
Tru9I TTAA 2 cut(s) 459, 501
TscAI CASTG 3 cut(s) 28, 70, 148
TspDTI ATGAA 4 cut(s) 209, 461, 489, 715
TspRI CASTG 3 cut(s) 28, 70, 148
VpaK11BI GGWCC 3 cut(s) 220, 276, 409
XagI CCTNNNNNAGG 1 cut(s) 334
XapI RAATTY 1 cut(s) 680
XceI RCATGY 1 cut(s) 424
XcmI CCANNNNNNNNNTGG 1 cut(s) 617
XspI CTAG 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.