MD02G1272500.v1.1

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001760
Physical Location & Seq
Reverse (-)
32693545 .. 32695512
1968 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1272500.v1.1.491

Sequence Viewer

Length: 642 bp
ATGAAACGGTGCAGAAGTACATCGCCAATTAATGTTGATGCCTCAGCAACTGGAAGTATCTATGATAGTTCAAGGCTGAGTGATACGGAGATATTTGACTTGGAATCAACTGCAATATCTTATATCAAAAGGCCTAGGATTGAGGTGAATCAAACCCTGTTTGAAGAAATTACAGCAATTAACCACCAACTAATAGACACTGTATTAGATGTCAGTGACGAAGATACCCCTCCAACAGTAGTCTCAGCTCAATTTAGAGGTGGTCAAGGAACCATTGTCCGGTGCTCCTTCATTCCCGTGACCATCGATCCCAGTCTGAGGTCACAGCACCCTTCAGCTCAAATGCTTCCAATCCAGCCTTTACGATTGCTTGTTCCTGCAAATTATCCATTGTGCTCTCCCATATTTCTAGACAAGTTGAGAGTGGAAGTCGGTGATGAGCTAGAAGATTTTTCAGCAAAGGTAAAATCAAAGCTGAATATGTCGCTCCGGAGGCTGATGGAACCCTTGTCACTTGGGGAGATAGCAAGGACGTGGGACATTTGTGTGCGAGCGGTTGTCGCAGAATATGCAGAGCAGAATGGGGGAGGAAACTTGAGTTCCAAGTATGGAGGGTGGGAAGACTGTATGAGTGCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

214

Amino Acids

23.52

Weight (kDa)

4.93

Isoelectric Point (pI)

52.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med15_C PF21539 116 - 184 4.5e-08 ARC105/Med15 mediator subunit C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 554
AccIII TCCGGA 1 cut(s) 489
AciI CCGC 1 cut(s) 554
AclWI GGATC 1 cut(s) 302
AcuI CTGAAG 1 cut(s) 318
AfaI GTAC 1 cut(s) 19
AfiI CCNNNNNNNGG 2 cut(s) 279, 318
AgsI TTSAA 2 cut(s) 72, 164
AjiI CACGTC 1 cut(s) 534
AluBI AGCT 4 cut(s) 248, 338, 442, 475
AluI AGCT 4 cut(s) 248, 338, 442, 475
Alw21I GWGCWC 2 cut(s) 287, 398
Alw26I GTCTC 1 cut(s) 247
AlwI GGATC 1 cut(s) 302
AlwNI CAGNNNCTG 1 cut(s) 50
Aor13HI TCCGGA 1 cut(s) 489
AoxI GGCC 1 cut(s) 131
ApeKI GCWGC 1 cut(s) 635
AseI ATTAAT 1 cut(s) 30
AspA2I CCTAGG 1 cut(s) 134
AsuHPI GGTGA 2 cut(s) 157, 446
AvrII CCTAGG 1 cut(s) 134
BbsI GAAGAC 1 cut(s) 627
Bbv12I GWGCWC 2 cut(s) 287, 398
BbvCI CCTCAGC 1 cut(s) 43
BbvI GCAGC 1 cut(s) 622
BccI CCATC 2 cut(s) 311, 493
BcoDI GTCTC 1 cut(s) 247
BfaI CTAG 3 cut(s) 135, 410, 443
BisI GCNGC 1 cut(s) 636
BlnI CCTAGG 1 cut(s) 134
BlsI GCNGC 1 cut(s) 637
BmgBI CACGTC 1 cut(s) 534
BmiI GGNNCC 2 cut(s) 271, 504
BmrI ACTGGG 1 cut(s) 306
BmsI GCATC 1 cut(s) 28
BmuI ACTGGG 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 627
Bpu10I CCTNAGC 1 cut(s) 43
BpuEI CTTGAG 1 cut(s) 616
Bsa29I ATCGAT 1 cut(s) 306
BsaJI CCNNGG 1 cut(s) 134
BsaWI WCCGGW 2 cut(s) 279, 489
Bsc4I CCNNNNNNNGG 2 cut(s) 279, 318
Bse1I ACTGG 2 cut(s) 55, 312
BseAI TCCGGA 1 cut(s) 489
BseCI ATCGAT 1 cut(s) 306
BseDI CCNNGG 1 cut(s) 134
BseLI CCNNNNNNNGG 2 cut(s) 279, 318
BseMII CTCAG 4 cut(s) 57, 68, 258, 308
BseNI ACTGG 2 cut(s) 55, 312
BseXI GCAGC 1 cut(s) 622
BsgI GTGCAG 1 cut(s) 31
BshFI GGCC 1 cut(s) 133
BshVI ATCGAT 1 cut(s) 306
BsiHKAI GWGCWC 2 cut(s) 287, 398
BsiSI CCGG 2 cut(s) 280, 490
BslFI GGGAC 1 cut(s) 551
BslI CCNNNNNNNGG 2 cut(s) 279, 318
BsmAI GTCTC 1 cut(s) 247
BsmFI GGGAC 1 cut(s) 551
BsnI GGCC 1 cut(s) 133
Bsp1286I GDGCHC 2 cut(s) 287, 398
Bsp13I TCCGGA 1 cut(s) 489
Bsp143I GATC 1 cut(s) 307
BspACI CCGC 1 cut(s) 554
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 4 cut(s) 56, 69, 257, 309
BspDI ATCGAT 1 cut(s) 306
BspEI TCCGGA 1 cut(s) 489
BspLI GGNNCC 2 cut(s) 271, 504
BspPI GGATC 1 cut(s) 302
BsrBI CCGCTC 1 cut(s) 554
BsrI ACTGG 2 cut(s) 55, 312
BssECI CCNNGG 1 cut(s) 134
BssMI GATC 1 cut(s) 307
BssT1I CCWWGG 1 cut(s) 134
Bst4CI ACNGT 4 cut(s) 9, 202, 238, 626
BstAPI GCANNNNNTGC 1 cut(s) 569
BstC8I GCNNGC 1 cut(s) 552
BstDEI CTNAG 4 cut(s) 43, 77, 244, 317
BstKTI GATC 1 cut(s) 310
BstMAI GTCTC 1 cut(s) 247
BstMBI GATC 1 cut(s) 307
BstMWI GCNNNNNNNGC 3 cut(s) 493, 560, 569
BstV1I GCAGC 1 cut(s) 622
BstV2I GAAGAC 1 cut(s) 627
Bsu15I ATCGAT 1 cut(s) 306
BsuRI GGCC 1 cut(s) 133
BsuTUI ATCGAT 1 cut(s) 306
BtgZI GCGATG 1 cut(s) 6
BtrI CACGTC 1 cut(s) 534
BtsIMutI CAGTG 2 cut(s) 198, 220
Cac8I GCNNGC 1 cut(s) 552
CaiI CAGNNNCTG 1 cut(s) 50
ClaI ATCGAT 1 cut(s) 306
Csp6I GTAC 1 cut(s) 18
CviJI RGCY 8 cut(s) 76, 133, 248, 338, 358, 442, 475, 496
CviKI_1 RGCY 8 cut(s) 76, 133, 248, 338, 358, 442, 475, 496
CviQI GTAC 1 cut(s) 18
DdeI CTNAG 4 cut(s) 43, 77, 244, 317
DpnI GATC 1 cut(s) 309
DpnII GATC 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 134
Eco147I AGGCCT 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 134
ErhI CCWWGG 1 cut(s) 134
FaiI YATR 7 cut(s) 63, 123, 404, 482, 570, 609, 629
FaqI GGGAC 1 cut(s) 551
Fnu4HI GCNGC 1 cut(s) 636
Fsp4HI GCNGC 1 cut(s) 636
FspBI CTAG 3 cut(s) 135, 410, 443
GluI GCNGC 1 cut(s) 636
HaeIII GGCC 1 cut(s) 133
HapII CCGG 2 cut(s) 280, 490
HinfI GANTC 2 cut(s) 104, 148
HpaII CCGG 2 cut(s) 280, 490
HphI GGTGA 2 cut(s) 157, 446
Hpy188I TCNGA 1 cut(s) 318
Hpy188III TCNNGA 2 cut(s) 410, 490
HpyAV CCTTC 2 cut(s) 298, 342
HpyCH4III ACNGT 4 cut(s) 9, 202, 238, 626
HpyCH4IV ACGT 1 cut(s) 533
HpyCH4V TGCA 4 cut(s) 12, 113, 380, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 493, 560, 569
HpyF3I CTNAG 4 cut(s) 43, 77, 244, 317
HpySE526I ACGT 1 cut(s) 533
Kpn2I TCCGGA 1 cut(s) 489
Kzo9I GATC 1 cut(s) 307
LmnI GCTCC 2 cut(s) 290, 492
LpnPI CCDG 7 cut(s) 36, 170, 293, 325, 368, 390, 503
Lsp1109I GCAGC 1 cut(s) 622
LweI GCATC 1 cut(s) 28
MaeI CTAG 3 cut(s) 135, 410, 443
MaeII ACGT 1 cut(s) 533
MaeIII GTNAC 4 cut(s) 215, 298, 321, 510
MalI GATC 1 cut(s) 309
MbiI CCGCTC 1 cut(s) 554
MboI GATC 1 cut(s) 307
MboII GAAGA 4 cut(s) 176, 233, 458, 632
MhlI GDGCHC 2 cut(s) 287, 398
MluCI AATT 5 cut(s) 27, 168, 177, 251, 382
MmeI TCCRAC 1 cut(s) 257
MnlI CCTC 8 cut(s) 52, 136, 240, 251, 312, 486, 581, 605
MroI TCCGGA 1 cut(s) 489
MseI TTAA 2 cut(s) 30, 180
MslI CAYNNNNRTG 2 cut(s) 296, 545
MspI CCGG 2 cut(s) 280, 490
MwoI GCNNNNNNNGC 3 cut(s) 493, 560, 569
NdeII GATC 1 cut(s) 307
NlaIV GGNNCC 2 cut(s) 271, 504
NmuCI GTSAC 4 cut(s) 215, 298, 321, 510
PceI AGGCCT 1 cut(s) 133
PfeI GAWTC 2 cut(s) 104, 148
PkrI GCNGC 1 cut(s) 637
PshBI ATTAAT 1 cut(s) 30
PspN4I GGNNCC 2 cut(s) 271, 504
PstNI CAGNNNCTG 1 cut(s) 50
RsaI GTAC 1 cut(s) 19
RsaNI GTAC 1 cut(s) 18
RseI CAYNNNNRTG 2 cut(s) 296, 545
SaqAI TTAA 2 cut(s) 30, 180
SatI GCNGC 1 cut(s) 636
Sau3AI GATC 1 cut(s) 307
SduI GDGCHC 2 cut(s) 287, 398
SetI ASST 9 cut(s) 147, 250, 262, 323, 340, 444, 465, 477, 536
SfaNI GCATC 1 cut(s) 28
SmiMI CAYNNNNRTG 2 cut(s) 296, 545
SmlI CTYRAG 1 cut(s) 595
SmoI CTYRAG 1 cut(s) 595
Sse9I AATT 5 cut(s) 27, 168, 177, 251, 382
SseBI AGGCCT 1 cut(s) 133
SsiI CCGC 1 cut(s) 554
SspMI CTAG 3 cut(s) 135, 410, 443
StuI AGGCCT 1 cut(s) 133
StyI CCWWGG 1 cut(s) 134
TaaI ACNGT 4 cut(s) 9, 202, 238, 626
TaiI ACGT 1 cut(s) 536
TaqI TCGA 1 cut(s) 306
TasI AATT 5 cut(s) 27, 168, 177, 251, 382
TatI WGTACW 1 cut(s) 17
TfiI GAWTC 2 cut(s) 104, 148
Tru1I TTAA 2 cut(s) 30, 180
Tru9I TTAA 2 cut(s) 30, 180
TscAI CASTG 2 cut(s) 205, 220
TseFI GTSAC 4 cut(s) 215, 298, 321, 510
TseI GCWGC 1 cut(s) 635
Tsp45I GTSAC 4 cut(s) 215, 298, 321, 510
TspDTI ATGAA 2 cut(s) 17, 280
TspGWI ACGGA 1 cut(s) 101
TspRI CASTG 2 cut(s) 205, 220
VspI ATTAAT 1 cut(s) 30
XbaI TCTAGA 1 cut(s) 409
XmaJI CCTAGG 1 cut(s) 134
XspI CTAG 3 cut(s) 135, 410, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.