pycom02g23360

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001760
Physical Location & Seq
Reverse (-)
21419674 .. 21421486
1813 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g23360.2

Sequence Viewer

Length: 1557 bp
ATGTATAAGACAATAACATGTAGGCTCCAGCAGCTCAACTCTGTCTCTCAACAGACAGAAACAGTCAGTATTGAGAAACTTAATGCATTGAGGAATAGGGTGAAGTCAATCATTTCTAATCTAAATTTGCCTAAAAGCAAAGTTGTACCTTCGTCCAAGGAGAACCTGGGTGTATATGAGAAGTTCATTACTTCTATTCTTAGCGTACGTGGAAGGGGAAATTCTGGTCCCTCACAGCAGCATGGACAATGTTCTTCTGATGTGAACTCTACCGGTCAATTTGTGCAACTTCATTCTCAAACTCCTCCAGTTCAGACACAGCAAGACAAAAAGAAGCCAACATTTCAGTCAGTGAACTCACAGGATTCTGCAACAACAATGCAGCGGAGCAATGTAACAAACTGGGTTAACGATTCTAATTCTGTATCTGAGTGCCCAACCCTGCAGCCGACTGAGACATATTCAGGAAAGATAGGCTCATTTAACGTAGTGCAGCAGGTCTCCTCAGGAACCTTAAATATTCCTAGAACCAATCTCCAACAAGCAAATGTTAAGACCTTGTTGTCACATAGCTCGGTGCATGGACCACAGTCAAATGTGAATGCTTTTGAGTCAAGTTGTAATACACTCCAAGAGATGCACCTGAAACAACCTAAAGAGGAGCGGGATGTGCAGACTCAGACCCTGAAGCAAGAACCTCAAGATTGGCATGAGATGCTGCAGCAGTCAGAACAAAAGCAAATTGAGCAACATCATAGCGGGCTGCTAAAGTTTCAAGCCAGAGCTTCATTTCCTACTGCTTCGCCCAAAACTCATGGTTCTTCCTGTTCACAAGTTCCTAGGTTTTCTACTATTGACAAGAAAAATATTCTGGTGCCTCTTACAGAAGCTAGAACCACTTTCTATCCCATAAACACACCCTCTACTAAAACTTCTTGTTTGGTTCCCTTGCCTTCATCGTCTGTCCCAGGAGATTTTGAGATACCTGTGTCTGATACTTCATCCCTATCAAATGCTGGAACTATTGGAGATCATGTAATAGATGGTGCACCAAAATCCACATCACCCGTTGCCATCAGCACTCCTGGGATATCTGCTTCACTCTTACTTGAAGAATCCAGTAACGACATTCATTGTAATACATCTACAATAATGTCTGATGAGTTGAGTGCTTCTAAGCAGCCTATTCAGCGCTTAATTAAAGTGGTGAACATGATGTCATCTAAAGCACTAAGTGCTGCTATTTTGGACATTGGCTCTGTAGTTTGTACAACTGATAGGATATCAGGATCAGCAACACTTACGGGATCTAGAGGTTTGGTTGGTGAGGATTTTGTGGGCATGACTAATTCTCGTTTGCAGAAGAGTTATTTGGCATGGCAAGATAAGACCTTTGGAACAAAAAATATGAAACGCTGCAGAAGTACGTCGCCAATTAATGTTGATGCCTCAGCAACTGGAAGTATCTATGATAGTTCAAGGCTGAGTGATACGGAGATATTTGACTTGGAATCAACTGCAATATCTTATATCAAAAGGCCTAAGATTGAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

519

Amino Acids

56.42

Weight (kDa)

8.72

Isoelectric Point (pI)

58.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 562
Acc36I ACCTGC 1 cut(s) 487
AccB1I GGYRCC 1 cut(s) 874
AccBSI CCGCTC 1 cut(s) 664
AciI CCGC 3 cut(s) 385, 664, 759
AclWI GGATC 2 cut(s) 1297, 1315
AcsI RAATTY 2 cut(s) 124, 220
AcuI CTGAAG 1 cut(s) 707
AdeI CACNNNGTG 1 cut(s) 1235
AfaI GTAC 4 cut(s) 147, 207, 1270, 1426
AfeI AGCGCT 1 cut(s) 1193
AflIII ACRYGT 1 cut(s) 17
AgeI ACCGGT 1 cut(s) 272
AgsI TTSAA 3 cut(s) 776, 1112, 1479
AjnI CCWGG 3 cut(s) 165, 967, 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 1355, 1387
AluBI AGCT 4 cut(s) 34, 573, 785, 890
AluI AGCT 4 cut(s) 34, 573, 785, 890
Alw21I GWGCWC 1 cut(s) 1051
Alw26I GTCTC 3 cut(s) 49, 449, 505
Alw44I GTGCAC 1 cut(s) 1047
AlwI GGATC 2 cut(s) 1297, 1315
AlwNI CAGNNNCTG 2 cut(s) 685, 1457
Aor51HI AGCGCT 1 cut(s) 1193
AoxI GGCC 1 cut(s) 1538
ApaLI GTGCAC 1 cut(s) 1047
ApoI RAATTY 2 cut(s) 124, 220
AseI ATTAAT 1 cut(s) 1437
AsiGI ACCGGT 1 cut(s) 272
AspA2I CCTAGG 1 cut(s) 839
AspLEI GCGC 1 cut(s) 1194
AspS9I GGNCC 2 cut(s) 227, 584
AsuHPI GGTGA 4 cut(s) 112, 1056, 1219, 1337
AvaII GGWCC 2 cut(s) 227, 584
AvrII CCTAGG 1 cut(s) 839
AxyI CCTNAGG 1 cut(s) 505
BaeGI GKGCMC 2 cut(s) 437, 1051
BaeI ACNNNNGTAYC 2 cut(s) 974, 1007
BanI GGYRCC 1 cut(s) 874
Bbv12I GWGCWC 1 cut(s) 1051
BbvCI CCTCAGC 1 cut(s) 1450
BccI CCATC 2 cut(s) 1037, 1082
BciT130I CCWGG 3 cut(s) 167, 969, 1086
BcoDI GTCTC 3 cut(s) 49, 449, 505
BfaI CTAG 4 cut(s) 525, 840, 891, 1311
BfmI CTRYAG 4 cut(s) 443, 719, 1260, 1417
BfoI RGCGCY 1 cut(s) 1195
BfuAI ACCTGC 1 cut(s) 487
BlnI CCTAGG 1 cut(s) 839
Bme1390I CCNGG 3 cut(s) 167, 969, 1086
Bme18I GGWCC 2 cut(s) 227, 584
BmgT120I GGNCC 2 cut(s) 227, 584
BmiI GGNNCC 5 cut(s) 26, 229, 511, 876, 945
BmrFI CCNGG 3 cut(s) 167, 969, 1086
BmrI ACTGGG 1 cut(s) 412
BmsI GCATC 3 cut(s) 627, 705, 1435
BmuI ACTGGG 1 cut(s) 412
BoxI GACNNNNGTC 1 cut(s) 589
BpmI CTGGAG 2 cut(s) 11, 291
Bpu10I CCTNAGC 1 cut(s) 1450
BpuEI CTTGAG 1 cut(s) 684
BsaAI YACGTR 1 cut(s) 209
BsaI GGTCTC 1 cut(s) 505
BsaJI CCNNGG 5 cut(s) 156, 166, 839, 967, 1085
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 2 cut(s) 1020, 1050
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 4 cut(s) 308, 407, 1119, 1462
Bse21I CCTNAGG 1 cut(s) 505
Bse3DI GCAATG 1 cut(s) 397
BseBI CCWGG 3 cut(s) 167, 969, 1086
BseDI CCNNGG 5 cut(s) 156, 166, 839, 967, 1085
BseGI GGATG 2 cut(s) 673, 1001
BseMI GCAATG 1 cut(s) 397
BseMII CTCAG 6 cut(s) 420, 444, 519, 692, 1464, 1475
BseNI ACTGG 4 cut(s) 308, 407, 1119, 1462
BseRI GAGGAG 3 cut(s) 294, 493, 674
BseSI GKGCMC 2 cut(s) 437, 1051
BsgI GTGCAG 2 cut(s) 512, 692
BshFI GGCC 1 cut(s) 1540
BshNI GGYRCC 1 cut(s) 874
BshTI ACCGGT 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 1051
BsiSI CCGG 1 cut(s) 273
BsiWI CGTACG 1 cut(s) 205
BslFI GGGAC 2 cut(s) 213, 950
BsmAI GTCTC 3 cut(s) 49, 449, 505
BsmFI GGGAC 2 cut(s) 213, 950
BsmI GAATGC 1 cut(s) 607
BsnI GGCC 1 cut(s) 1540
Bso31I GGTCTC 1 cut(s) 505
Bsp1286I GDGCHC 2 cut(s) 437, 1051
Bsp1407I TGTACA 1 cut(s) 1268
Bsp143I GATC 3 cut(s) 1030, 1289, 1307
BspACI CCGC 3 cut(s) 385, 664, 759
BspANI GGCC 1 cut(s) 1540
BspCNI CTCAG 6 cut(s) 421, 445, 518, 691, 1463, 1476
BspLI GGNNCC 5 cut(s) 26, 229, 511, 876, 945
BspMAI CTGCAG 3 cut(s) 447, 723, 1421
BspMI ACCTGC 1 cut(s) 487
BspPI GGATC 2 cut(s) 1297, 1315
BspT107I GGYRCC 1 cut(s) 874
BspTNI GGTCTC 1 cut(s) 505
BsrBI CCGCTC 1 cut(s) 664
BsrDI GCAATG 1 cut(s) 397
BsrFI RCCGGY 1 cut(s) 272
BsrGI TGTACA 1 cut(s) 1268
BsrI ACTGG 4 cut(s) 308, 407, 1119, 1462
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 5 cut(s) 156, 166, 839, 967, 1085
BssMI GATC 3 cut(s) 1030, 1289, 1307
BssT1I CCWWGG 2 cut(s) 156, 839
Bst2UI CCWGG 3 cut(s) 167, 969, 1086
Bst4CI ACNGT 2 cut(s) 64, 591
Bst6I CTCTTC 1 cut(s) 1358
BstAPI GCANNNNNTGC 2 cut(s) 715, 1235
BstAUI TGTACA 1 cut(s) 1268
BstBAI YACGTR 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 761
BstF5I GGATG 2 cut(s) 673, 1001
BstH2I RGCGCY 1 cut(s) 1195
BstHHI GCGC 1 cut(s) 1194
BstKTI GATC 3 cut(s) 1033, 1292, 1310
BstMAI GTCTC 3 cut(s) 49, 449, 505
BstMBI GATC 3 cut(s) 1030, 1289, 1307
BstMWI GCNNNNNNNGC 6 cut(s) 31, 670, 715, 745, 1189, 1235
BstNI CCWGG 3 cut(s) 167, 969, 1086
BstNSI RCATGY 1 cut(s) 21
BstPAI GACNNNNGTC 1 cut(s) 589
BstSCI CCNGG 3 cut(s) 165, 967, 1084
BstSFI CTRYAG 4 cut(s) 443, 719, 1260, 1417
BstSLI GKGCMC 2 cut(s) 437, 1051
BstX2I RGATCY 1 cut(s) 1307
BstYI RGATCY 1 cut(s) 1307
Bsu36I CCTNAGG 1 cut(s) 505
BsuRI GGCC 1 cut(s) 1540
BtsCI GGATG 2 cut(s) 673, 1001
BtsIMutI CAGTG 1 cut(s) 357
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 1 cut(s) 761
CaiI CAGNNNCTG 2 cut(s) 685, 1457
CfoI GCGC 1 cut(s) 1194
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 2 cut(s) 227, 584
Csp6I GTAC 4 cut(s) 146, 206, 1269, 1425
CspAI ACCGGT 1 cut(s) 272
CviAII CATG 9 cut(s) 18, 242, 581, 710, 815, 1034, 1213, 1342, 1377
CviQI GTAC 4 cut(s) 146, 206, 1269, 1425
DpnI GATC 3 cut(s) 1032, 1291, 1309
DpnII GATC 3 cut(s) 1030, 1289, 1307
DraIII CACNNNGTG 1 cut(s) 1235
DrdI GACNNNNNNGTC 1 cut(s) 562
DseDI GACNNNNNNGTC 1 cut(s) 562
Eam1104I CTCTTC 1 cut(s) 1358
EarI CTCTTC 1 cut(s) 1358
Eco130I CCWWGG 2 cut(s) 156, 839
Eco147I AGGCCT 1 cut(s) 1540
Eco31I GGTCTC 1 cut(s) 505
Eco32I GATATC 2 cut(s) 1092, 1284
Eco47I GGWCC 2 cut(s) 227, 584
Eco47III AGCGCT 1 cut(s) 1193
Eco57I CTGAAG 1 cut(s) 707
Eco81I CCTNAGG 1 cut(s) 505
EcoRII CCWGG 3 cut(s) 165, 967, 1084
EcoRV GATATC 2 cut(s) 1092, 1284
EcoT14I CCWWGG 2 cut(s) 156, 839
EcoT22I ATGCAT 1 cut(s) 88
ErhI CCWWGG 2 cut(s) 156, 839
FaeI CATG 9 cut(s) 21, 245, 584, 713, 818, 1037, 1216, 1345, 1380
FaqI GGGAC 2 cut(s) 213, 950
FatI CATG 9 cut(s) 17, 241, 580, 709, 814, 1033, 1212, 1341, 1376
FauI CCCGC 2 cut(s) 657, 752
FokI GGATG 2 cut(s) 680, 988
FspBI CTAG 4 cut(s) 525, 840, 891, 1311
GlaI GCGC 1 cut(s) 1193
GsuI CTGGAG 2 cut(s) 11, 291
HaeII RGCGCY 1 cut(s) 1195
HaeIII GGCC 1 cut(s) 1540
HapII CCGG 1 cut(s) 273
HhaI GCGC 1 cut(s) 1194
Hin1II CATG 9 cut(s) 21, 245, 584, 713, 818, 1037, 1216, 1345, 1380
Hin6I GCGC 1 cut(s) 1192
HinP1I GCGC 1 cut(s) 1192
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HinfI GANTC 6 cut(s) 365, 413, 611, 676, 1115, 1511
HpaI GTTAAC 1 cut(s) 409
HpaII CCGG 1 cut(s) 273
HphI GGTGA 4 cut(s) 112, 1056, 1219, 1337
Hpy166II GTNNAC 6 cut(s) 265, 355, 409, 830, 1049, 1210
Hpy188I TCNGA 7 cut(s) 259, 315, 430, 681, 730, 994, 1159
Hpy188III TCNNGA 5 cut(s) 465, 507, 701, 1287, 1311
Hpy8I GTNNAC 6 cut(s) 265, 355, 409, 830, 1049, 1210
Hpy99I CGWCG 1 cut(s) 1432
HpyAV CCTTC 3 cut(s) 159, 207, 963
HpyCH4III ACNGT 2 cut(s) 64, 591
HpyCH4IV ACGT 3 cut(s) 208, 486, 1427
HpyF10VI GCNNNNNNNGC 6 cut(s) 31, 670, 715, 745, 1189, 1235
HpySE526I ACGT 3 cut(s) 208, 486, 1427
Hsp92II CATG 9 cut(s) 21, 245, 584, 713, 818, 1037, 1216, 1345, 1380
HspAI GCGC 1 cut(s) 1192
KspAI GTTAAC 1 cut(s) 409
Kzo9I GATC 3 cut(s) 1030, 1289, 1307
LmnI GCTCC 3 cut(s) 30, 387, 661
LweI GCATC 3 cut(s) 627, 705, 1435
MaeI CTAG 4 cut(s) 525, 840, 891, 1311
MaeII ACGT 3 cut(s) 208, 486, 1427
MaeIII GTNAC 3 cut(s) 394, 564, 1121
MalI GATC 3 cut(s) 1032, 1291, 1309
MbiI CCGCTC 1 cut(s) 664
MboI GATC 3 cut(s) 1030, 1289, 1307
MboII GAAGA 4 cut(s) 246, 813, 1124, 1375
MflI RGATCY 1 cut(s) 1307
MhlI GDGCHC 2 cut(s) 437, 1051
MluCI AATT 8 cut(s) 124, 220, 278, 418, 741, 1197, 1348, 1434
MlyI GAGTC 2 cut(s) 620, 670
MmeI TCCRAC 1 cut(s) 562
Mph1103I ATGCAT 1 cut(s) 88
MseI TTAA 8 cut(s) 81, 408, 483, 515, 552, 1196, 1200, 1437
MspA1I CMGCKG 1 cut(s) 385
MspI CCGG 1 cut(s) 273
MspR9I CCNGG 3 cut(s) 167, 969, 1086
Mva1269I GAATGC 1 cut(s) 607
MvaI CCWGG 3 cut(s) 167, 969, 1086
MwoI GCNNNNNNNGC 6 cut(s) 31, 670, 715, 745, 1189, 1235
NdeII GATC 3 cut(s) 1030, 1289, 1307
NlaIII CATG 9 cut(s) 21, 245, 584, 713, 818, 1037, 1216, 1345, 1380
NlaIV GGNNCC 5 cut(s) 26, 229, 511, 876, 945
NmuCI GTSAC 1 cut(s) 564
NsiI ATGCAT 1 cut(s) 88
NspI RCATGY 1 cut(s) 21
PacI TTAATTAA 1 cut(s) 1200
PceI AGGCCT 1 cut(s) 1540
PciI ACATGT 1 cut(s) 17
PctI GAATGC 1 cut(s) 607
PfeI GAWTC 4 cut(s) 365, 413, 1115, 1511
Pfl23II CGTACG 1 cut(s) 205
PinAI ACCGGT 1 cut(s) 272
PleI GAGTC 2 cut(s) 619, 670
PpsI GAGTC 2 cut(s) 619, 670
Ppu21I YACGTR 1 cut(s) 209
PscI ACATGT 1 cut(s) 17
PshAI GACNNNNGTC 1 cut(s) 589
PshBI ATTAAT 1 cut(s) 1437
Psp6I CCWGG 3 cut(s) 165, 967, 1084
PspGI CCWGG 3 cut(s) 165, 967, 1084
PspLI CGTACG 1 cut(s) 205
PspN4I GGNNCC 5 cut(s) 26, 229, 511, 876, 945
PspPI GGNCC 2 cut(s) 227, 584
PstI CTGCAG 3 cut(s) 447, 723, 1421
PstNI CAGNNNCTG 2 cut(s) 685, 1457
PsuI RGATCY 1 cut(s) 1307
RsaI GTAC 4 cut(s) 147, 207, 1270, 1426
RsaNI GTAC 4 cut(s) 146, 206, 1269, 1425
SaqAI TTAA 8 cut(s) 81, 408, 483, 515, 552, 1196, 1200, 1437
Sau3AI GATC 3 cut(s) 1030, 1289, 1307
Sau96I GGNCC 2 cut(s) 227, 584
SchI GAGTC 2 cut(s) 620, 670
ScrFI CCNGG 3 cut(s) 167, 969, 1086
SduI GDGCHC 2 cut(s) 437, 1051
SfaNI GCATC 3 cut(s) 627, 705, 1435
SfcI CTRYAG 4 cut(s) 443, 719, 1260, 1417
SinI GGWCC 2 cut(s) 227, 584
SmlI CTYRAG 1 cut(s) 699
SmoI CTYRAG 1 cut(s) 699
Sse9I AATT 8 cut(s) 124, 220, 278, 418, 741, 1197, 1348, 1434
SseBI AGGCCT 1 cut(s) 1540
SsiI CCGC 3 cut(s) 385, 664, 759
SspI AATATT 2 cut(s) 520, 868
SspMI CTAG 4 cut(s) 525, 840, 891, 1311
StuI AGGCCT 1 cut(s) 1540
StyD4I CCNGG 3 cut(s) 165, 967, 1084
StyI CCWWGG 2 cut(s) 156, 839
TaaI ACNGT 2 cut(s) 64, 591
TaiI ACGT 3 cut(s) 211, 489, 1430
TasI AATT 8 cut(s) 124, 220, 278, 418, 741, 1197, 1348, 1434
TatI WGTACW 1 cut(s) 1268
TfiI GAWTC 4 cut(s) 365, 413, 1115, 1511
Tru1I TTAA 8 cut(s) 81, 408, 483, 515, 552, 1196, 1200, 1437
Tru9I TTAA 8 cut(s) 81, 408, 483, 515, 552, 1196, 1200, 1437
TscAI CASTG 1 cut(s) 357
TseFI GTSAC 1 cut(s) 564
Tsp45I GTSAC 1 cut(s) 564
TspDTI ATGAA 7 cut(s) 175, 281, 777, 945, 990, 1121, 1424
TspGWI ACGGA 1 cut(s) 1508
TspRI CASTG 1 cut(s) 357
VneI GTGCAC 1 cut(s) 1047
VpaK11BI GGWCC 2 cut(s) 227, 584
VspI ATTAAT 1 cut(s) 1437
XapI RAATTY 2 cut(s) 124, 220
XbaI TCTAGA 1 cut(s) 1310
XceI RCATGY 1 cut(s) 21
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
XmaJI CCTAGG 1 cut(s) 839
XspI CTAG 4 cut(s) 525, 840, 891, 1311
Zsp2I ATGCAT 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.