Rroxscaffold_4G00321130

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001760
Physical Location & Seq
Forward (+)
50325675 .. 50328089
2415 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00321130.1

Sequence Viewer

Length: 2415 bp
ATGAAAGTGGAAAACCAGAGCTCCAGTCCTTTACCCTTTAGGTCTAGGAATCGGCAAATCCATTATTATTTAGGAAACCCTGTAAAAGAAAAAACTATTTGTATTGCAGCAGGGAAACCAATTTCTGCAATCCCACCACCAGCAGAAACTCTCAATAATCTGTCGTGCATGCAGCTTCCATTTTCTGATCTTCAGCAGAATCATCAGATGTCTCCTTTTCAGCAGACATCACAATCTGCGATTAACAATCATCCACAGACAGGTTTTAGGCAAATAAACTCATCACAACATGCTCCTTATATTCATCAGAATGCATCATCAATTCCACAGAGTCTGCTAATGCAATCGCAGAAGCATGTTGTGATGAGGAGAAGATTAGCTGCCACCACTCTACAACAGAATCAGTCAACTTGCCTCGACTTTCCGCAGCAACAACACATTAACACATACCAGCTGAATGATGCACAACAGCAGTTACATAGACAGAACCTTAATCTTTCAGGTCCGCATCATGAGCAATTGAGCCAAAGAAACATTATCTCTATACAGCAACAAACTCTTGAGAGACAGTTGAATTCTTCACAGGTAGAGAAGATGCAAAGGCAGCATAACCGAGTAGAACAAGATGAGCAGCAAACACATCAAATGGCAGGAATGCCAGCTTTCACATTATTAATGGAAGGGGAGGTCTACCGAAAGGTCCAAATGCTAAAAAGCAAGTATGAAAGACCACTGAAAGTTATCTTCCAAAGGTTCACATATTGTCTCCAGAACCTAGATAAACACAGTATCAACAAACTTGAAGCAGTAAACAGCTTGTATCAGACTGTCAAGTCTATCCTTAGGATGCTGAATGTGCCGGAAAGCGACACTATGCCTACTACCAAGGAGAAGTTGGGTCTACTTGAGAAGAAAATCGAAGATATTCTCCACCCATGTGGAAACCAACAGCGTCAATTGGTGCAAATTATTGATTCTCGGACTCTTCAAGCTCAGACAAACCAAGAGGAAAAGAAGCCACCAACGCAGACAGTGAATATAGAATGTTTTGCAACAAAGATGCAGCAGAGCAATGTGACAAACTTGATGATGAACGATTCTCAGCCTCTGATCGAGAAACATGGAAGAAAACATTTGCCAAGAAACTTGACTAGGAAAGACTCAGATCAAGCAAGTTCGTCTGGGTACTTGACAATGCCTACAAGTAGTCCCCAACAACAAAATGTTATGACCTTGTTGTCACAAAACCCCATTCAGTCAAATGTCAATAATTTGGAGTCAAGATTTAGTGTACCTCGATATGTGCACCTGAAACAATCTAAAGAACAGCAGGATGTGCAAACTCAAAAACCAAAACAGCAATTACTGCAGGAGAATCACCGGCTGGTGAAGTTGCAGTCTGAAGCTTTTCCTAATCCTTTGCGGGAAGATCATAATTCTTCCAGTCAACAAGTTCCTAAACATACTTGTCAGAGTGAAAAGATCAACCTTCCAATGCATCTTACAAAAGTTAGCAGTCCTTTCAATTCACCCTCTAGAATTTCTTCTTTGGCTCCCAAGGCTTTATCACCTATGCCAGTGGACTCTGAGAAACCTAAGTCAGATACTCTGTCCAACTCAAATGCTGGAAATATTGGAGATGAACAAGTAAAAGGTGCAACAGAAGCCCCATCATTAAGTACTCCTTTACTTGAAAAGTCCAGCGATGACATATGTAGTAATAAGTCCACAGTAGATTCTGATGCGGTGCAGCCTATTCATCAATTAATTAATGTGGTGAACTCCATGTCATCTAAAGCACCAAGTGCTTCTGCTTCAGTCATTGCTTCAGTAGCGTGTACGACTGATAGGATGAAAATGAAGCGCCCGAGAAGTACATTGCCAATTATTGATGCTGATTCAGAAAGTGGGATATGTCATATGATGAAAAGGCCCAGGCTTGAGGTGAACCAAACCCTCTTAGAGGAAATTAGAGCAATCAACAAACGACTAATAGACACTGTACTAGATATTAGTGACCAAGAGACTGCTCCATCAGTTAAGGATGGTGAACGAACTATTGTCAGGTGCTCCTTCGTTGGTGTGGCCATCAGTCCTAATCTGATGTCATCTCCAATGTTTCCACTCCAGCCTTTAAGATTGCTTGTGCCTGCAAACTATCCATTCAGCTCTCCGATATTTCTGGACAAGATGACAGTGCAAGTCAGGGATGAGCTGGAAGATCTCTCAGCAAAGGTCATGTCAAAGCTAAGTCACTCTCTCCGGACCCTAGCAGAGCCCTTATCTCTTGGGGAGATAGCAAGGGTATGGGATACATGCGCCCGAGCGGTTGTCTGTGAATACGCACAACAGTCTGGGGGAGGAAGCTTCAGTTCCAAGTATGGAACCTGGGAAGATTTCCTCACTGTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

804

Amino Acids

90.51

Weight (kDa)

9.0

Isoelectric Point (pI)

69.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 832, 1237
AccBSI CCGCTC 1 cut(s) 2327
AccI GTMKAC 2 cut(s) 690, 901
AccIII TCCGGA 1 cut(s) 2262
AciI CCGC 5 cut(s) 425, 506, 1423, 1745, 2327
AcoI YGGCCR 1 cut(s) 2085
AcsI RAATTY 2 cut(s) 574, 1539
AcuI CTGAAG 5 cut(s) 176, 1422, 1800, 1812, 2353
AdeI CACNNNGTG 1 cut(s) 1805
AfaI GTAC 6 cut(s) 1187, 1293, 1681, 1840, 1876, 2004
AfiI CCNNNNNNNGG 2 cut(s) 260, 1963
AgsI TTSAA 5 cut(s) 574, 803, 989, 1525, 1694
AjnI CCWGG 2 cut(s) 1934, 2387
AleI CACNNNNGTG 1 cut(s) 936
Alw21I GWGCWC 3 cut(s) 23, 1308, 2072
Alw26I GTCTC 4 cut(s) 216, 559, 770, 2018
Alw44I GTGCAC 1 cut(s) 1304
AlwNI CAGNNNCTG 2 cut(s) 334, 1108
Ama87I CYCGRG 2 cut(s) 1867, 2322
Aor13HI TCCGGA 1 cut(s) 2262
AoxI GGCC 2 cut(s) 1931, 2085
ApaLI GTGCAC 1 cut(s) 1304
ApeKI GCWGC 8 cut(s) 107, 172, 380, 427, 604, 631, 1063, 1750
ApoI RAATTY 2 cut(s) 574, 1539
ArsI GACNNNNNNTTYG 2 cut(s) 2225, 2257
AseI ATTAAT 3 cut(s) 674, 1766, 1770
Asp700I GAANNNNTTC 3 cut(s) 924, 1407, 1543
AspLEI GCGC 2 cut(s) 1866, 2321
AspS9I GGNCC 4 cut(s) 503, 700, 1932, 2265
AsuHPI GGTGA 7 cut(s) 1370, 1399, 1521, 1560, 1789, 1957, 2060
AvaI CYCGRG 2 cut(s) 1867, 2322
AvaII GGWCC 3 cut(s) 503, 700, 2265
AxyI CCTNAGG 1 cut(s) 842
BaeGI GKGCMC 1 cut(s) 1308
BalI TGGCCA 1 cut(s) 2087
BanII GRGCYC 2 cut(s) 23, 2280
Bbv12I GWGCWC 3 cut(s) 23, 1308, 2072
BbvI GCAGC 8 cut(s) 119, 184, 367, 439, 616, 643, 1075, 1762
BccI CCATC 4 cut(s) 1678, 2039, 2041, 2096
BciT130I CCWGG 2 cut(s) 1936, 2389
BciVI GTATCC 1 cut(s) 2305
BcoDI GTCTC 4 cut(s) 216, 559, 770, 2018
BfaI CTAG 6 cut(s) 45, 776, 1152, 1536, 2006, 2270
BfmI CTRYAG 1 cut(s) 1367
BfoI RGCGCY 1 cut(s) 1867
BfuI GTATCC 1 cut(s) 2305
BglII AGATCT 1 cut(s) 2221
BisI GCNGC 8 cut(s) 108, 173, 381, 428, 605, 632, 1064, 1751
BlsI GCNGC 8 cut(s) 109, 174, 382, 429, 606, 633, 1065, 1752
BmcAI AGTACT 1 cut(s) 1681
Bme1390I CCNGG 2 cut(s) 1936, 2389
Bme18I GGWCC 3 cut(s) 503, 700, 2265
BmeT110I CYCGRG 2 cut(s) 1867, 2322
BmgT120I GGNCC 4 cut(s) 503, 700, 1932, 2265
BmiI GGNNCC 3 cut(s) 1554, 2267, 2386
BmrFI CCNGG 2 cut(s) 1936, 2389
BmsI GCATC 9 cut(s) 323, 451, 517, 585, 837, 1050, 1507, 1732, 1882
BpmI CTGGAG 3 cut(s) 7, 752, 2111
BpuEI CTTGAG 3 cut(s) 581, 926, 1961
BsaJI CCNNGG 4 cut(s) 885, 1557, 1934, 2388
BsaWI WCCGGW 1 cut(s) 2262
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 1963
Bse118I RCCGGY 1 cut(s) 1380
Bse1I ACTGG 3 cut(s) 24, 1443, 1577
Bse21I CCTNAGG 1 cut(s) 842
Bse3DI GCAATG 3 cut(s) 1078, 1821, 1877
BseAI TCCGGA 1 cut(s) 2262
BseBI CCWGG 2 cut(s) 1936, 2389
BseDI CCNNGG 4 cut(s) 885, 1557, 1934, 2388
BseGI GGATG 6 cut(s) 250, 852, 1339, 1857, 2050, 2215
BseLI CCNNNNNNNGG 2 cut(s) 260, 1963
BseMI GCAATG 3 cut(s) 1078, 1821, 1877
BseMII CTCAG 5 cut(s) 1007, 1115, 1176, 1578, 2241
BseNI ACTGG 3 cut(s) 24, 1443, 1577
BseRI GAGGAG 1 cut(s) 382
BseSI GKGCMC 1 cut(s) 1308
BseXI GCAGC 8 cut(s) 119, 184, 367, 439, 616, 643, 1075, 1762
BsgI GTGCAG 1 cut(s) 1769
BshFI GGCC 2 cut(s) 1933, 2087
BsiHKAI GWGCWC 3 cut(s) 23, 1308, 2072
BsiHKCI CYCGRG 2 cut(s) 1867, 2322
BsiSI CCGG 3 cut(s) 860, 1381, 2263
BslFI GGGAC 1 cut(s) 1194
BslI CCNNNNNNNGG 2 cut(s) 260, 1963
BsmAI GTCTC 4 cut(s) 216, 559, 770, 2018
BsmFI GGGAC 1 cut(s) 1194
BsmI GAATGC 2 cut(s) 316, 660
BsnI GGCC 2 cut(s) 1933, 2087
BsoBI CYCGRG 2 cut(s) 1867, 2322
Bsp1286I GDGCHC 4 cut(s) 23, 1308, 2072, 2280
Bsp13I TCCGGA 1 cut(s) 2262
Bsp143I GATC 6 cut(s) 187, 1110, 1165, 1429, 1482, 2221
BspACI CCGC 5 cut(s) 425, 506, 1423, 1745, 2327
BspANI GGCC 2 cut(s) 1933, 2087
BspCNI CTCAG 5 cut(s) 1006, 1114, 1175, 1579, 2240
BspEI TCCGGA 1 cut(s) 2262
BspHI TCATGA 1 cut(s) 511
BspLI GGNNCC 3 cut(s) 1554, 2267, 2386
BspMAI CTGCAG 1 cut(s) 1371
BsrBI CCGCTC 1 cut(s) 2327
BsrDI GCAATG 3 cut(s) 1078, 1821, 1877
BsrFI RCCGGY 1 cut(s) 1380
BsrI ACTGG 3 cut(s) 24, 1443, 1577
BssAI RCCGGY 1 cut(s) 1380
BssECI CCNNGG 4 cut(s) 885, 1557, 1934, 2388
BssMI GATC 6 cut(s) 187, 1110, 1165, 1429, 1482, 2221
BssT1I CCWWGG 2 cut(s) 885, 1557
Bst2UI CCWGG 2 cut(s) 1936, 2389
Bst4CI ACNGT 9 cut(s) 570, 788, 829, 1033, 1732, 2002, 2197, 2352, 2407
Bst6I CTCTTC 1 cut(s) 990
BstAPI GCANNNNNTGC 3 cut(s) 1336, 1366, 1805
BstC8I GCNNGC 3 cut(s) 170, 660, 2151
BstDEI CTNAG 9 cut(s) 842, 993, 1101, 1162, 1587, 1596, 1960, 2227, 2249
BstENI CCTNNNNNAGG 1 cut(s) 1961
BstF5I GGATG 6 cut(s) 250, 852, 1339, 1857, 2050, 2215
BstH2I RGCGCY 1 cut(s) 1867
BstHHI GCGC 2 cut(s) 1866, 2321
BstKTI GATC 6 cut(s) 190, 1113, 1168, 1432, 1485, 2224
BstMAI GTCTC 4 cut(s) 216, 559, 770, 2018
BstMBI GATC 6 cut(s) 187, 1110, 1165, 1429, 1482, 2221
BstNI CCWGG 2 cut(s) 1936, 2389
BstNSI RCATGY 4 cut(s) 172, 293, 359, 2319
BstSCI CCNGG 2 cut(s) 1934, 2387
BstSFI CTRYAG 1 cut(s) 1367
BstSLI GKGCMC 1 cut(s) 1308
BstV1I GCAGC 8 cut(s) 119, 184, 367, 439, 616, 643, 1075, 1762
BstX2I RGATCY 1 cut(s) 2221
BstXI CCANNNNNNTGG 1 cut(s) 938
BstYI RGATCY 1 cut(s) 2221
Bsu36I CCTNAGG 1 cut(s) 842
BsuI GTATCC 1 cut(s) 2305
BsuRI GGCC 2 cut(s) 1933, 2087
BtgZI GCGATG 1 cut(s) 1719
BtsCI GGATG 6 cut(s) 250, 852, 1339, 1857, 2050, 2215
BtsIMutI CAGTG 6 cut(s) 731, 1038, 1584, 1998, 2202, 2403
Cac8I GCNNGC 3 cut(s) 170, 660, 2151
CaiI CAGNNNCTG 2 cut(s) 334, 1108
CciI TCATGA 1 cut(s) 511
CfoI GCGC 2 cut(s) 1866, 2321
Cfr10I RCCGGY 1 cut(s) 1380
Cfr13I GGNCC 4 cut(s) 503, 700, 1932, 2265
CseI GACGC 1 cut(s) 941
Csp6I GTAC 6 cut(s) 1186, 1292, 1680, 1839, 1875, 2003
CviAII CATG 9 cut(s) 169, 290, 356, 512, 936, 1121, 1786, 2239, 2316
CviQI GTAC 6 cut(s) 1186, 1292, 1680, 1839, 1875, 2003
DdeI CTNAG 9 cut(s) 842, 993, 1101, 1162, 1587, 1596, 1960, 2227, 2249
DpnI GATC 6 cut(s) 189, 1112, 1167, 1431, 1484, 2223
DpnII GATC 6 cut(s) 187, 1110, 1165, 1429, 1482, 2221
DraIII CACNNNGTG 1 cut(s) 1805
DrdI GACNNNNNNGTC 2 cut(s) 832, 1237
DseDI GACNNNNNNGTC 2 cut(s) 832, 1237
EaeI YGGCCR 1 cut(s) 2085
Eam1104I CTCTTC 1 cut(s) 990
EarI CTCTTC 1 cut(s) 990
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 2 cut(s) 885, 1557
Eco24I GRGCYC 2 cut(s) 23, 2280
Eco47I GGWCC 3 cut(s) 503, 700, 2265
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 5 cut(s) 176, 1422, 1800, 1812, 2353
Eco81I CCTNAGG 1 cut(s) 842
Eco88I CYCGRG 2 cut(s) 1867, 2322
EcoICRI GAGCTC 1 cut(s) 21
EcoNI CCTNNNNNAGG 1 cut(s) 1961
EcoRI GAATTC 1 cut(s) 574
EcoRII CCWGG 2 cut(s) 1934, 2387
EcoT14I CCWWGG 2 cut(s) 885, 1557
EcoT22I ATGCAT 2 cut(s) 316, 1500
EcoT38I GRGCYC 2 cut(s) 23, 2280
ErhI CCWWGG 2 cut(s) 885, 1557
FaeI CATG 9 cut(s) 172, 293, 359, 515, 939, 1124, 1789, 2242, 2319
FalI AAGNNNNNCTT 2 cut(s) 1669, 1701
FaqI GGGAC 1 cut(s) 1194
FatI CATG 9 cut(s) 168, 289, 355, 511, 935, 1120, 1785, 2238, 2315
FauI CCCGC 1 cut(s) 1416
FauNDI CATATG 2 cut(s) 1712, 1920
FblI GTMKAC 2 cut(s) 690, 901
Fnu4HI GCNGC 8 cut(s) 108, 173, 381, 428, 605, 632, 1064, 1751
FokI GGATG 6 cut(s) 237, 859, 1346, 1864, 2057, 2222
FriOI GRGCYC 2 cut(s) 23, 2280
Fsp4HI GCNGC 8 cut(s) 108, 173, 381, 428, 605, 632, 1064, 1751
FspBI CTAG 6 cut(s) 45, 776, 1152, 1536, 2006, 2270
GlaI GCGC 2 cut(s) 1865, 2320
GluI GCNGC 8 cut(s) 108, 173, 381, 428, 605, 632, 1064, 1751
GsuI CTGGAG 3 cut(s) 7, 752, 2111
HaeII RGCGCY 1 cut(s) 1867
HaeIII GGCC 2 cut(s) 1933, 2087
HapII CCGG 3 cut(s) 860, 1381, 2263
HgaI GACGC 1 cut(s) 941
HhaI GCGC 2 cut(s) 1866, 2321
Hin1II CATG 9 cut(s) 172, 293, 359, 515, 939, 1124, 1789, 2242, 2319
Hin6I GCGC 2 cut(s) 1864, 2319
HinP1I GCGC 2 cut(s) 1864, 2319
HincII GTYRAC 2 cut(s) 408, 1448
HindII GTYRAC 2 cut(s) 408, 1448
HindIII AAGCTT 2 cut(s) 1404, 2365
HpaII CCGG 3 cut(s) 860, 1381, 2263
HphI GGTGA 7 cut(s) 1370, 1399, 1521, 1560, 1789, 1957, 2060
Hpy188III TCNNGA 8 cut(s) 512, 560, 769, 1114, 1281, 1536, 2183, 2263
HpyAV CCTTC 3 cut(s) 674, 1499, 2083
HpyCH4III ACNGT 9 cut(s) 570, 788, 829, 1033, 1732, 2002, 2197, 2352, 2407
HpyF3I CTNAG 9 cut(s) 842, 993, 1101, 1162, 1587, 1596, 1960, 2227, 2249
Hsp92II CATG 9 cut(s) 172, 293, 359, 515, 939, 1124, 1789, 2242, 2319
HspAI GCGC 2 cut(s) 1864, 2319
Kpn2I TCCGGA 1 cut(s) 2262
Kzo9I GATC 6 cut(s) 187, 1110, 1165, 1429, 1482, 2221
LmnI GCTCC 5 cut(s) 26, 298, 1558, 2035, 2075
Lsp1109I GCAGC 8 cut(s) 119, 184, 367, 439, 616, 643, 1075, 1762
LweI GCATC 9 cut(s) 323, 451, 517, 585, 837, 1050, 1507, 1732, 1882
MaeI CTAG 6 cut(s) 45, 776, 1152, 1536, 2006, 2270
MaeIII GTNAC 5 cut(s) 474, 1075, 1239, 2015, 2252
MalI GATC 6 cut(s) 189, 1112, 1167, 1431, 1484, 2223
MbiI CCGCTC 1 cut(s) 2327
MboI GATC 6 cut(s) 187, 1110, 1165, 1429, 1482, 2221
MfeI CAATTG 2 cut(s) 518, 956
MflI RGATCY 1 cut(s) 2221
MhlI GDGCHC 4 cut(s) 23, 1308, 2072, 2280
MlsI TGGCCA 1 cut(s) 2087
MluNI TGGCCA 1 cut(s) 2087
MlyI GAGTC 5 cut(s) 340, 976, 1154, 1286, 1577
MmeI TCCRAC 1 cut(s) 1638
Mox20I TGGCCA 1 cut(s) 2087
Mph1103I ATGCAT 2 cut(s) 316, 1500
MroI TCCGGA 1 cut(s) 2262
MroXI GAANNNNTTC 3 cut(s) 924, 1407, 1543
MscI TGGCCA 1 cut(s) 2087
MslI CAYNNNNRTG 2 cut(s) 309, 936
Msp20I TGGCCA 1 cut(s) 2087
MspA1I CMGCKG 1 cut(s) 454
MspI CCGG 3 cut(s) 860, 1381, 2263
MspR9I CCNGG 2 cut(s) 1936, 2389
MunI CAATTG 2 cut(s) 518, 956
Mva1269I GAATGC 2 cut(s) 316, 660
MvaI CCWGG 2 cut(s) 1936, 2389
NdeI CATATG 2 cut(s) 1712, 1920
NdeII GATC 6 cut(s) 187, 1110, 1165, 1429, 1482, 2221
NlaIII CATG 9 cut(s) 172, 293, 359, 515, 939, 1124, 1789, 2242, 2319
NlaIV GGNNCC 3 cut(s) 1554, 2267, 2386
NmuCI GTSAC 4 cut(s) 1075, 1239, 2015, 2252
NsiI ATGCAT 2 cut(s) 316, 1500
NspI RCATGY 4 cut(s) 172, 293, 359, 2319
OliI CACNNNNGTG 1 cut(s) 936
PacI TTAATTAA 1 cut(s) 1770
PaeI GCATGC 1 cut(s) 172
PagI TCATGA 1 cut(s) 511
PctI GAATGC 2 cut(s) 316, 660
PdmI GAANNNNTTC 3 cut(s) 924, 1407, 1543
PfeI GAWTC 8 cut(s) 49, 199, 400, 974, 1097, 1375, 1736, 1898
PkrI GCNGC 8 cut(s) 109, 174, 382, 429, 606, 633, 1065, 1752
PleI GAGTC 5 cut(s) 339, 976, 1154, 1285, 1577
PpsI GAGTC 5 cut(s) 339, 976, 1154, 1285, 1577
PshBI ATTAAT 3 cut(s) 674, 1766, 1770
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 2 cut(s) 1934, 2387
PspGI CCWGG 2 cut(s) 1934, 2387
PspN4I GGNNCC 3 cut(s) 1554, 2267, 2386
PspPI GGNCC 4 cut(s) 503, 700, 1932, 2265
PstI CTGCAG 1 cut(s) 1371
PstNI CAGNNNCTG 2 cut(s) 334, 1108
PsuI RGATCY 1 cut(s) 2221
PvuII CAGCTG 1 cut(s) 454
RsaI GTAC 6 cut(s) 1187, 1293, 1681, 1840, 1876, 2004
RsaNI GTAC 6 cut(s) 1186, 1292, 1680, 1839, 1875, 2003
RseI CAYNNNNRTG 2 cut(s) 309, 936
SacI GAGCTC 1 cut(s) 23
SatI GCNGC 8 cut(s) 108, 173, 381, 428, 605, 632, 1064, 1751
Sau3AI GATC 6 cut(s) 187, 1110, 1165, 1429, 1482, 2221
Sau96I GGNCC 4 cut(s) 503, 700, 1932, 2265
ScaI AGTACT 1 cut(s) 1681
SchI GAGTC 5 cut(s) 340, 976, 1154, 1286, 1577
ScrFI CCNGG 2 cut(s) 1936, 2389
SduI GDGCHC 4 cut(s) 23, 1308, 2072, 2280
SfaNI GCATC 9 cut(s) 323, 451, 517, 585, 837, 1050, 1507, 1732, 1882
SfcI CTRYAG 1 cut(s) 1367
SinI GGWCC 3 cut(s) 503, 700, 2265
SmiMI CAYNNNNRTG 2 cut(s) 309, 936
SmlI CTYRAG 3 cut(s) 560, 905, 1940
SmoI CTYRAG 3 cut(s) 560, 905, 1940
SphI GCATGC 1 cut(s) 172
SsiI CCGC 5 cut(s) 425, 506, 1423, 1745, 2327
SspI AATATT 1 cut(s) 1633
SspMI CTAG 6 cut(s) 45, 776, 1152, 1536, 2006, 2270
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 2 cut(s) 1934, 2387
StyI CCWWGG 2 cut(s) 885, 1557
TaaI ACNGT 9 cut(s) 570, 788, 829, 1033, 1732, 2002, 2197, 2352, 2407
TaqI TCGA 4 cut(s) 417, 918, 1113, 1297
TatI WGTACW 3 cut(s) 1679, 1874, 2002
TfiI GAWTC 8 cut(s) 49, 199, 400, 974, 1097, 1375, 1736, 1898
TscAI CASTG 6 cut(s) 738, 1038, 1584, 2005, 2202, 2410
TseFI GTSAC 4 cut(s) 1075, 1239, 2015, 2252
TseI GCWGC 8 cut(s) 107, 172, 380, 427, 604, 631, 1063, 1750
Tsp45I GTSAC 4 cut(s) 1075, 1239, 2015, 2252
TspDTI ATGAA 9 cut(s) 17, 293, 738, 1106, 1656, 1748, 1868, 1874, 1940
TspRI CASTG 6 cut(s) 738, 1038, 1584, 2005, 2202, 2410
VneI GTGCAC 1 cut(s) 1304
VpaK11BI GGWCC 3 cut(s) 503, 700, 2265
VspI ATTAAT 3 cut(s) 674, 1766, 1770
XagI CCTNNNNNAGG 1 cut(s) 1961
XapI RAATTY 2 cut(s) 574, 1539
XbaI TCTAGA 1 cut(s) 1535
XceI RCATGY 4 cut(s) 172, 293, 359, 2319
XcmI CCANNNNNNNNNTGG 1 cut(s) 892
XmiI GTMKAC 2 cut(s) 690, 901
XmnI GAANNNNTTC 3 cut(s) 924, 1407, 1543
XspI CTAG 6 cut(s) 45, 776, 1152, 1536, 2006, 2270
ZrmI AGTACT 1 cut(s) 1681
Zsp2I ATGCAT 2 cut(s) 316, 1500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.