Rh4CG036300

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0001760
Physical Location & Seq
Forward (+)
7343454 .. 7358425
14972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG036300.1

Sequence Viewer

Length: 789 bp
ATGAAGAAGAGAAGAGTCGGTTATGTGGGTCAGGCAAGGGCGCCTCTTTCCACAGCTTCCCCCGAGAGATACTTTGCCTGGCAGGAAACAACTGTTGGAACAAGGATAATGAAGCGTCACAGAAGTATGCCAATTAGCTATGGGGCTTCAGAAAATAGCAATATAAATGATAGCTTGTCACATCTAAGTGATATGGAAAATTTAGACTTGGACTCACCTGCAATATCTTATAGCAAACGGCATAGGCTTGAGGTGAATCATCTCTTAGAAGAGATTACCGCTATCAACCAACAACTAATAGACACTGTTTTAGATATTAGAGATGGAGAGACTATTCCATCAGTAGCCTCAGCTCCAATTCTCGATGGTGAAGGAACCATTGTTAGGTGTTCCTTCATTGCTGTAGCCGTTGCTCCTAATCTGAAGTCTGAATGTGTTCCAGCGCCAATGCTTGCAATCCAGCCTCTGCGATTGCTTGTTCTCGCAAATTATCCTTTGTGCTCTCCTATATTTATGGACAAGTTGAAAGTGGAAGTCAGATACTTCTCAACACCTTACTCACTCCCCAAACTTGATATGGTTGCGGTTCCTGAATTTTCTGGTGGGGCCATGGAGAATTATGGTTTGATCACATACTGTGAAAATGGACTGCTTTTTTATCCTTTGCATTCCACAGCAGCAAGAAAGCAAAGATTCCACAGCAGCAAGAAAGCAAAGAATGACAAAAGGATGTCCATCCCGACAAAGCATAACTTCCACCCTAACAAAGCACAGATAATGGGGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

262

Amino Acids

29.25

Weight (kDa)

9.1

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M1 PF01433 174 - 232 9.9e-14 Peptidase family M1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 226
Acc36I ACCTGC 1 cut(s) 226
AccB1I GGYRCC 1 cut(s) 40
AciI CCGC 2 cut(s) 279, 584
AcsI RAATTY 2 cut(s) 199, 593
AcuI CTGAAG 2 cut(s) 132, 443
AcyI GRCGYC 1 cut(s) 41
AfiI CCNNNNNNNGG 1 cut(s) 384
AgsI TTSAA 1 cut(s) 526
AjnI CCWGG 1 cut(s) 77
AjuI GAANNNNNNNTTGG 2 cut(s) 78, 110
AluBI AGCT 4 cut(s) 56, 138, 174, 353
AluI AGCT 4 cut(s) 56, 138, 174, 353
Alw21I GWGCWC 1 cut(s) 503
Alw26I GTCTC 1 cut(s) 323
AlwNI CAGNNNCTG 1 cut(s) 466
Ama87I CYCGRG 1 cut(s) 62
AoxI GGCC 2 cut(s) 606, 783
ApeKI GCWGC 2 cut(s) 677, 702
ApoI RAATTY 2 cut(s) 199, 593
Asp700I GAANNNNTTC 1 cut(s) 435
AspLEI GCGC 2 cut(s) 43, 445
AspS9I GGNCC 2 cut(s) 606, 783
AsuHPI GGTGA 3 cut(s) 207, 265, 380
AvaI CYCGRG 1 cut(s) 62
BanI GGYRCC 1 cut(s) 40
Bbv12I GWGCWC 1 cut(s) 503
BbvCI CCTCAGC 1 cut(s) 349
BbvI GCAGC 2 cut(s) 689, 714
BccI CCATC 4 cut(s) 317, 346, 359, 743
BceAI ACGGC 2 cut(s) 254, 392
BciT130I CCWGG 1 cut(s) 79
BclI TGATCA 1 cut(s) 627
BcoDI GTCTC 1 cut(s) 323
BfmI CTRYAG 1 cut(s) 402
BfoI RGCGCY 2 cut(s) 44, 446
BfuAI ACCTGC 1 cut(s) 226
BisI GCNGC 2 cut(s) 678, 703
BlsI GCNGC 2 cut(s) 679, 704
Bme1390I CCNGG 1 cut(s) 79
BmeT110I CYCGRG 1 cut(s) 62
BmgT120I GGNCC 2 cut(s) 606, 783
BmiI GGNNCC 5 cut(s) 42, 376, 588, 607, 784
BmrFI CCNGG 1 cut(s) 79
Bpu10I CCTNAGC 1 cut(s) 349
BpuEI CTTGAG 1 cut(s) 269
BsaBI GATNNNNATC 1 cut(s) 734
BsaHI GRCGYC 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 609
Bsc4I CCNNNNNNNGG 1 cut(s) 384
Bse3DI GCAATG 1 cut(s) 396
Bse8I GATNNNNATC 1 cut(s) 734
BseBI CCWGG 1 cut(s) 79
BseDI CCNNGG 1 cut(s) 609
BseGI GGATG 2 cut(s) 735, 735
BseJI GATNNNNATC 1 cut(s) 734
BseLI CCNNNNNNNGG 1 cut(s) 384
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 1 cut(s) 363
BseXI GCAGC 2 cut(s) 689, 714
BshFI GGCC 2 cut(s) 608, 785
BshNI GGYRCC 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 503
BsiHKCI CYCGRG 1 cut(s) 62
BslI CCNNNNNNNGG 1 cut(s) 384
BsmAI GTCTC 1 cut(s) 323
BsmI GAATGC 1 cut(s) 667
BsnI GGCC 2 cut(s) 608, 785
BsoBI CYCGRG 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 503
Bsp143I GATC 1 cut(s) 627
Bsp19I CCATGG 1 cut(s) 609
BspACI CCGC 2 cut(s) 279, 584
BspANI GGCC 2 cut(s) 608, 785
BspCNI CTCAG 1 cut(s) 362
BspLI GGNNCC 5 cut(s) 42, 376, 588, 607, 784
BspMI ACCTGC 1 cut(s) 226
BspT107I GGYRCC 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 396
BssECI CCNNGG 1 cut(s) 609
BssMI GATC 1 cut(s) 627
BssNI GRCGYC 1 cut(s) 41
BssT1I CCWWGG 1 cut(s) 609
Bst2UI CCWGG 1 cut(s) 79
Bst4CI ACNGT 3 cut(s) 94, 307, 638
Bst6I CTCTTC 3 cut(s) 2, 7, 264
BstACI GRCGYC 1 cut(s) 41
BstC8I GCNNGC 1 cut(s) 453
BstDEI CTNAG 3 cut(s) 185, 265, 349
BstDSI CCRYGG 1 cut(s) 609
BstF5I GGATG 2 cut(s) 735, 735
BstH2I RGCGCY 2 cut(s) 44, 446
BstHHI GCGC 2 cut(s) 43, 445
BstKTI GATC 1 cut(s) 630
BstMAI GTCTC 1 cut(s) 323
BstMBI GATC 1 cut(s) 627
BstNI CCWGG 1 cut(s) 79
BstSCI CCNGG 1 cut(s) 77
BstSFI CTRYAG 1 cut(s) 402
BstV1I GCAGC 2 cut(s) 689, 714
BsuRI GGCC 2 cut(s) 608, 785
BtgI CCRYGG 1 cut(s) 609
BtsCI GGATG 2 cut(s) 735, 735
BtsIMutI CAGTG 1 cut(s) 303
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 1 cut(s) 453
CaiI CAGNNNCTG 1 cut(s) 466
CfoI GCGC 2 cut(s) 43, 445
Cfr13I GGNCC 2 cut(s) 606, 783
CseI GACGC 1 cut(s) 104
CviAII CATG 1 cut(s) 610
DdeI CTNAG 3 cut(s) 185, 265, 349
DinI GGCGCC 1 cut(s) 42
DpnI GATC 1 cut(s) 629
DpnII GATC 1 cut(s) 627
Eam1104I CTCTTC 3 cut(s) 2, 7, 264
EarI CTCTTC 3 cut(s) 2, 7, 264
Eco130I CCWWGG 1 cut(s) 609
Eco57I CTGAAG 2 cut(s) 132, 443
Eco88I CYCGRG 1 cut(s) 62
EcoO109I RGGNCCY 1 cut(s) 783
EcoRII CCWGG 1 cut(s) 77
EcoT14I CCWWGG 1 cut(s) 609
EgeI GGCGCC 1 cut(s) 42
EheI GGCGCC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 609
FaeI CATG 1 cut(s) 613
FalI AAGNNNNNCTT 2 cut(s) 737, 769
FatI CATG 1 cut(s) 609
FbaI TGATCA 1 cut(s) 627
Fnu4HI GCNGC 2 cut(s) 678, 703
FokI GGATG 2 cut(s) 722, 742
Fsp4HI GCNGC 2 cut(s) 678, 703
GlaI GCGC 2 cut(s) 42, 444
GluI GCNGC 2 cut(s) 678, 703
HaeII RGCGCY 2 cut(s) 44, 446
HaeIII GGCC 2 cut(s) 608, 785
HgaI GACGC 1 cut(s) 104
HhaI GCGC 2 cut(s) 43, 445
Hin1I GRCGYC 1 cut(s) 41
Hin1II CATG 1 cut(s) 613
Hin6I GCGC 2 cut(s) 41, 443
HinP1I GCGC 2 cut(s) 41, 443
HinfI GANTC 4 cut(s) 15, 212, 256, 693
HphI GGTGA 3 cut(s) 207, 265, 380
Hpy188I TCNGA 4 cut(s) 151, 423, 430, 539
Hpy188III TCNNGA 3 cut(s) 362, 590, 739
HpyAV CCTTC 2 cut(s) 365, 403
HpyCH4III ACNGT 3 cut(s) 94, 307, 638
HpyCH4V TGCA 3 cut(s) 221, 455, 667
HpyF3I CTNAG 3 cut(s) 185, 265, 349
Hsp92I GRCGYC 1 cut(s) 41
Hsp92II CATG 1 cut(s) 613
HspAI GCGC 2 cut(s) 41, 443
KasI GGCGCC 1 cut(s) 40
Ksp22I TGATCA 1 cut(s) 627
Kzo9I GATC 1 cut(s) 627
LmnI GCTCC 2 cut(s) 358, 418
LpnPI CCDG 9 cut(s) 17, 64, 68, 91, 231, 453, 473, 585, 603
Lsp1109I GCAGC 2 cut(s) 689, 714
MaeIII GTNAC 2 cut(s) 116, 177
MalI GATC 1 cut(s) 629
MboI GATC 1 cut(s) 627
MboII GAAGA 4 cut(s) 16, 19, 24, 281
MhlI GDGCHC 1 cut(s) 503
MluCI AATT 6 cut(s) 132, 199, 357, 487, 593, 616
Mly113I GGCGCC 1 cut(s) 41
MlyI GAGTC 2 cut(s) 24, 206
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 4 cut(s) 54, 244, 358, 474
MroXI GAANNNNTTC 1 cut(s) 435
MslI CAYNNNNRTG 1 cut(s) 186
MspR9I CCNGG 1 cut(s) 79
Mva1269I GAATGC 1 cut(s) 667
MvaI CCWGG 1 cut(s) 79
NarI GGCGCC 1 cut(s) 41
NcoI CCATGG 1 cut(s) 609
NdeII GATC 1 cut(s) 627
NlaIII CATG 1 cut(s) 613
NlaIV GGNNCC 5 cut(s) 42, 376, 588, 607, 784
NmuCI GTSAC 2 cut(s) 116, 177
PaqCI CACCTGC 1 cut(s) 226
PctI GAATGC 1 cut(s) 667
PdmI GAANNNNTTC 1 cut(s) 435
PfeI GAWTC 2 cut(s) 256, 693
PkrI GCNGC 2 cut(s) 679, 704
PleI GAGTC 2 cut(s) 23, 206
PluTI GGCGCC 1 cut(s) 44
PpsI GAGTC 2 cut(s) 23, 206
Psp6I CCWGG 1 cut(s) 77
PspGI CCWGG 1 cut(s) 77
PspN4I GGNNCC 5 cut(s) 42, 376, 588, 607, 784
PspPI GGNCC 2 cut(s) 606, 783
PstNI CAGNNNCTG 1 cut(s) 466
RseI CAYNNNNRTG 1 cut(s) 186
SatI GCNGC 2 cut(s) 678, 703
Sau3AI GATC 1 cut(s) 627
Sau96I GGNCC 2 cut(s) 606, 783
SchI GAGTC 2 cut(s) 24, 206
ScrFI CCNGG 1 cut(s) 79
SduI GDGCHC 1 cut(s) 503
SetI ASST 8 cut(s) 58, 140, 176, 220, 255, 355, 389, 556
SfcI CTRYAG 1 cut(s) 402
SfoI GGCGCC 1 cut(s) 42
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 6 cut(s) 132, 199, 357, 487, 593, 616
SsiI CCGC 2 cut(s) 279, 584
SspDI GGCGCC 1 cut(s) 40
StyD4I CCNGG 1 cut(s) 77
StyI CCWWGG 1 cut(s) 609
TaaI ACNGT 3 cut(s) 94, 307, 638
TaqI TCGA 1 cut(s) 363
TasI AATT 6 cut(s) 132, 199, 357, 487, 593, 616
TfiI GAWTC 2 cut(s) 256, 693
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 2 cut(s) 116, 177
TseI GCWGC 2 cut(s) 677, 702
Tsp45I GTSAC 2 cut(s) 116, 177
TspDTI ATGAA 3 cut(s) 17, 125, 385
TspRI CASTG 1 cut(s) 310
XapI RAATTY 2 cut(s) 199, 593
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XmnI GAANNNNTTC 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.