RLG00000029895

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001760
Physical Location & Seq
Forward (+)
50954778 .. 50955454
677 bp
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UTR
Exon/CDS
Intron
RLM00000029895

Sequence Viewer

Length: 312 bp
ATGTCATCTCCAATGTTTCCACTCCAGCCTTTAAGATTGCTTGTGCCTGCAAACTATCCATTCAGCTCTCCTATATTTCTGGACAAGTTGACAGTGCAAGTCAGGGATGAGCTGGAAGATCTCTCAGCAAAGGTCATGTCAAAGCTAAGTCACTCTCTCCGGACCCTAGCGGAACCCTTATCTCTTGGGGAGATAGCAAGGGTATGGGATACATGCGCCCGAGCGGTTGTCTGTGAATACGCACAACAGTCTGGTGGAGGAAGCTTCAGTTCCAAGTATGGAACCTGGGAAGATTTCCTCACTGTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

104

Amino Acids

11.4

Weight (kDa)

5.24

Isoelectric Point (pI)

57.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med15_C PF21539 9 - 74 2.1e-06 ARC105/Med15 mediator subunit C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 224
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 2 cut(s) 170, 224
AcuI CTGAAG 1 cut(s) 250
AjnI CCWGG 1 cut(s) 284
AluBI AGCT 4 cut(s) 66, 112, 145, 264
AluI AGCT 4 cut(s) 66, 112, 145, 264
Ama87I CYCGRG 1 cut(s) 219
Aor13HI TCCGGA 1 cut(s) 159
ArsI GACNNNNNNTTYG 2 cut(s) 122, 154
AspLEI GCGC 1 cut(s) 218
AspS9I GGNCC 1 cut(s) 162
AvaI CYCGRG 1 cut(s) 219
AvaII GGWCC 1 cut(s) 162
BciT130I CCWGG 1 cut(s) 286
BciVI GTATCC 1 cut(s) 202
BfaI CTAG 1 cut(s) 167
BfuI GTATCC 1 cut(s) 202
BglII AGATCT 1 cut(s) 118
Bme1390I CCNGG 1 cut(s) 286
Bme18I GGWCC 1 cut(s) 162
BmeT110I CYCGRG 1 cut(s) 219
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 3 cut(s) 164, 174, 283
BmrFI CCNGG 1 cut(s) 286
BpmI CTGGAG 1 cut(s) 8
BsaJI CCNNGG 1 cut(s) 285
BsaWI WCCGGW 1 cut(s) 159
BseAI TCCGGA 1 cut(s) 159
BseBI CCWGG 1 cut(s) 286
BseDI CCNNGG 1 cut(s) 285
BseGI GGATG 1 cut(s) 112
BseMII CTCAG 1 cut(s) 138
BsiHKCI CYCGRG 1 cut(s) 219
BsiSI CCGG 1 cut(s) 160
BsoBI CYCGRG 1 cut(s) 219
Bsp13I TCCGGA 1 cut(s) 159
Bsp143I GATC 1 cut(s) 118
BspACI CCGC 2 cut(s) 170, 224
BspCNI CTCAG 1 cut(s) 137
BspEI TCCGGA 1 cut(s) 159
BspLI GGNNCC 3 cut(s) 164, 174, 283
BsrBI CCGCTC 1 cut(s) 224
BssECI CCNNGG 1 cut(s) 285
BssMI GATC 1 cut(s) 118
Bst2UI CCWGG 1 cut(s) 286
Bst4CI ACNGT 3 cut(s) 94, 249, 304
BstC8I GCNNGC 1 cut(s) 48
BstDEI CTNAG 2 cut(s) 124, 146
BstF5I GGATG 1 cut(s) 112
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 1 cut(s) 121
BstMBI GATC 1 cut(s) 118
BstNI CCWGG 1 cut(s) 286
BstNSI RCATGY 1 cut(s) 216
BstSCI CCNGG 1 cut(s) 284
BstX2I RGATCY 1 cut(s) 118
BstYI RGATCY 1 cut(s) 118
BsuI GTATCC 1 cut(s) 202
BtsCI GGATG 1 cut(s) 112
BtsIMutI CAGTG 2 cut(s) 99, 300
Cac8I GCNNGC 1 cut(s) 48
CfoI GCGC 1 cut(s) 218
Cfr13I GGNCC 1 cut(s) 162
CviAII CATG 2 cut(s) 136, 213
CviJI RGCY 5 cut(s) 28, 66, 112, 145, 264
CviKI_1 RGCY 5 cut(s) 28, 66, 112, 145, 264
DdeI CTNAG 2 cut(s) 124, 146
DpnI GATC 1 cut(s) 120
DpnII GATC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 162
Eco57I CTGAAG 1 cut(s) 250
Eco88I CYCGRG 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 284
FaeI CATG 2 cut(s) 139, 216
FaiI YATR 5 cut(s) 74, 137, 205, 214, 279
FatI CATG 2 cut(s) 135, 212
FokI GGATG 1 cut(s) 119
FspBI CTAG 1 cut(s) 167
GlaI GCGC 1 cut(s) 217
GsuI CTGGAG 1 cut(s) 8
HapII CCGG 1 cut(s) 160
HhaI GCGC 1 cut(s) 218
Hin1II CATG 2 cut(s) 139, 216
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HincII GTYRAC 1 cut(s) 90
HindII GTYRAC 1 cut(s) 90
HindIII AAGCTT 1 cut(s) 262
HpaII CCGG 1 cut(s) 160
Hpy166II GTNNAC 1 cut(s) 90
Hpy188III TCNNGA 2 cut(s) 80, 160
Hpy8I GTNNAC 1 cut(s) 90
HpyCH4III ACNGT 3 cut(s) 94, 249, 304
HpyCH4V TGCA 2 cut(s) 50, 97
HpyF3I CTNAG 2 cut(s) 124, 146
Hsp92II CATG 2 cut(s) 139, 216
HspAI GCGC 1 cut(s) 216
Kpn2I TCCGGA 1 cut(s) 159
Kzo9I GATC 1 cut(s) 118
LpnPI CCDG 9 cut(s) 38, 60, 65, 88, 98, 173, 237, 271, 298
MaeI CTAG 1 cut(s) 167
MaeIII GTNAC 1 cut(s) 149
MalI GATC 1 cut(s) 120
MbiI CCGCTC 1 cut(s) 224
MboI GATC 1 cut(s) 118
MboII GAAGA 2 cut(s) 128, 302
MflI RGATCY 1 cut(s) 118
MnlI CCTC 2 cut(s) 251, 308
MroI TCCGGA 1 cut(s) 159
MseI TTAA 2 cut(s) 32, 310
MspI CCGG 1 cut(s) 160
MspR9I CCNGG 1 cut(s) 286
MvaI CCWGG 1 cut(s) 286
NdeII GATC 1 cut(s) 118
NlaIII CATG 2 cut(s) 139, 216
NlaIV GGNNCC 3 cut(s) 164, 174, 283
NmuCI GTSAC 1 cut(s) 149
NspI RCATGY 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 284
PspGI CCWGG 1 cut(s) 284
PspN4I GGNNCC 3 cut(s) 164, 174, 283
PspPI GGNCC 1 cut(s) 162
PsuI RGATCY 1 cut(s) 118
SaqAI TTAA 2 cut(s) 32, 310
Sau3AI GATC 1 cut(s) 118
Sau96I GGNCC 1 cut(s) 162
ScrFI CCNGG 1 cut(s) 286
SetI ASST 6 cut(s) 68, 114, 135, 147, 266, 287
SinI GGWCC 1 cut(s) 162
SsiI CCGC 2 cut(s) 170, 224
SspMI CTAG 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 284
TaaI ACNGT 3 cut(s) 94, 249, 304
Tru1I TTAA 2 cut(s) 32, 310
Tru9I TTAA 2 cut(s) 32, 310
TscAI CASTG 2 cut(s) 99, 307
TseFI GTSAC 1 cut(s) 149
Tsp45I GTSAC 1 cut(s) 149
TspRI CASTG 2 cut(s) 99, 307
VpaK11BI GGWCC 1 cut(s) 162
XceI RCATGY 1 cut(s) 216
XspI CTAG 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.