MD02G1272800.v1.1

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001760
Physical Location & Seq
Reverse (-)
32706283 .. 32711104
4822 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1272800.v1.1.491

Sequence Viewer

Length: 1638 bp
ATGACCTTTACCGGTGACAAATCTACACGTACACTAATTCCTTGCTTATCAAGCTTACCAACCTACGCACGTATACAGCCAATAATATATGAGAAGCACACACTCGTACACTTCACACAACGGCGGGAAAGGAAAGAGTCTTTTGAGCACACAGTCGATGATCAGTCGCCCTTTCCTCTGCCGCCAGTCCTCTGCAAAACCCTTTTTCTGATTACTTCAATCTGCGATTTCGCAAGACGTTTTGTGAAGACCCAAAAAAGGCACGCTCAAGCTGAACTCAATATGGATCCAGTCAATTGGAGAACTAAATTTCCTGCAGAGTCTCGAGAAAAAGTTGTGAACAAAGTTTTCAGTACCTTAATGAAGCGTGTTCCATTTTCTGAGCACGAGAAACATATGTCTGATTACAAGAGGATTTCTGTAAATTTCGAGGAAAAGGTTTATATGGTTTCCAGAGACCAGCAAGAATATATACTGCGGATCAAATCAAAGTTGGAAGAATTGGAATCTTCGCCAAAAAACTTGAATCCAGGTGGCGCGCAAGGCCAATCAATTTCTGTTCAATCCACAGCTCCATCTCAAGCACATGAACAACATTCACTGCCTAGTGTTCGAGATAACATTGCATCTGGTGGAGGTTTTACTAACTTAGCATCTGCAAAGCCCACTGTCAGTGTTACGCCTCAGGTTCCTTTGCCAAATAATGCTAGTGAAAATTCCAATACGCAGAATGTCTTTGGTAATTCACAGAGGTCTGTACGAAATTTATCAGGACGTGGGCAACCTTATAATATTACTGCCAATAATATGAGACAGGAGCAAGGGAGACAAAATTCACCCCAGATTGTTGCTCAGCAGAACCATGAGCTGCAAAATCCTCATCTTTGTAAGCAGGTCTGGCAGGAAAATATCTCACCCTCATATGTAACCCAGCAAAAGCAACTACAGCCGCAACACCACTACCAACAGAAACTCTTAAAACCTACTCAGCAACAGACCTCTCAACAATCTATGTTGCAAACTCCTGTCATGCGCCCTTCTGAGACATGTGTTTCTCCACTTTCTGATATTTGGCAGGACCCGATGTCTTCTTTCTATCAGACAACACAGTTGAGGATTAAGAATCAGTCACAGATAGCCTTTAGGCAAACAAACCAATCACAAGTGACTCCGGATATTCATCAAAATGTATCATCAAGTTCACAGAACCCATTAATGCAATCACAAATGCAAGCACATTTGATAGGGAGAAAGTTGACTGCTACTAATACGCAACAAAGTCACATAGATGGGCAACCAAATGGCGTCCTTAACTTGCAGCAGCAACAGCCCAGGTCACCATTCCAACAGAATAACGTACAACAACGGTTTCAGTTACAGAAAACAAATCTTTCAAGTGCATATCATGAGCAATTGGGCGAAAGAAACAATATTTCTGTGCTACAACAAAGCTCACAGCTGCCAAATAAAGATCAAGTACCACAACCACACTCTCTTGAGAGACGACTGAACACTCAAACACAGCCAGAGAAGATGAAACAATTGCCAAATCCATCGGCACAAGATCAGCAGCAAGCATATCAAACATCAGGTAAAAGTACTGTGAAACTGAATCATGTTTCAGCAGAAAAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

546

Amino Acids

62.19

Weight (kDa)

9.73

Isoelectric Point (pI)

68.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 99 - 174 1e-17 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 789
Acc36I ACCTGC 1 cut(s) 883
AccI GTMKAC 1 cut(s) 73
AccII CGCG 1 cut(s) 539
AccIII TCCGGA 1 cut(s) 1171
AciI CCGC 4 cut(s) 124, 182, 478, 950
AclWI GGATC 3 cut(s) 281, 294, 488
AcsI RAATTY 5 cut(s) 308, 424, 715, 763, 832
AcyI GRCGYC 1 cut(s) 1305
AfaI GTAC 7 cut(s) 31, 108, 355, 759, 1359, 1479, 1600
AfiI CCNNNNNNNGG 1 cut(s) 258
AflIII ACRYGT 2 cut(s) 26, 1046
AgeI ACCGGT 1 cut(s) 11
AgsI TTSAA 4 cut(s) 219, 526, 563, 1395
AhdI GACNNNNNGTC 1 cut(s) 1084
AjiI CACGTC 1 cut(s) 776
AjnI CCWGG 2 cut(s) 529, 1331
AloI GAACNNNNNNTCC 2 cut(s) 295, 327
AluBI AGCT 6 cut(s) 54, 272, 572, 868, 1452, 1459
AluI AGCT 6 cut(s) 54, 272, 572, 868, 1452, 1459
Alw21I GWGCWC 2 cut(s) 150, 387
Alw26I GTCTC 6 cut(s) 327, 450, 805, 820, 1037, 1495
AlwI GGATC 3 cut(s) 281, 294, 488
Ama87I CYCGRG 1 cut(s) 324
Aor13HI TCCGGA 1 cut(s) 1171
AoxI GGCC 1 cut(s) 544
ApeKI GCWGC 5 cut(s) 868, 1318, 1321, 1459, 1570
ApoI RAATTY 5 cut(s) 308, 424, 715, 763, 832
AseI ATTAAT 1 cut(s) 1214
AsiGI ACCGGT 1 cut(s) 11
AspLEI GCGC 3 cut(s) 539, 541, 1035
AspS9I GGNCC 1 cut(s) 1078
AsuHPI GGTGA 4 cut(s) 26, 828, 906, 1329
AvaI CYCGRG 1 cut(s) 324
AvaII GGWCC 1 cut(s) 1078
AxyI CCTNAGG 1 cut(s) 684
BamHI GGATCC 1 cut(s) 286
BauI CACGAG 1 cut(s) 386
BbsI GAAGAC 2 cut(s) 254, 1080
Bbv12I GWGCWC 2 cut(s) 150, 387
BbvI GCAGC 5 cut(s) 855, 1330, 1333, 1446, 1582
BccI CCATC 3 cut(s) 583, 1283, 1561
BceAI ACGGC 1 cut(s) 137
BciT130I CCWGG 2 cut(s) 531, 1333
BclI TGATCA 1 cut(s) 160
BcoDI GTCTC 6 cut(s) 327, 450, 805, 820, 1037, 1495
BfaI CTAG 2 cut(s) 606, 708
BfmI CTRYAG 2 cut(s) 315, 944
BfuAI ACCTGC 1 cut(s) 883
BisI GCNGC 7 cut(s) 182, 869, 950, 1319, 1322, 1460, 1571
BlpI GCTNAGC 1 cut(s) 852
BlsI GCNGC 7 cut(s) 183, 870, 951, 1320, 1323, 1461, 1572
BmcAI AGTACT 1 cut(s) 1600
Bme1390I CCNGG 2 cut(s) 531, 1333
Bme18I GGWCC 1 cut(s) 1078
BmeRI GACNNNNNGTC 1 cut(s) 1084
BmeT110I CYCGRG 1 cut(s) 324
BmgBI CACGTC 1 cut(s) 776
BmgT120I GGNCC 1 cut(s) 1078
BmiI GGNNCC 3 cut(s) 288, 690, 1080
BmrFI CCNGG 2 cut(s) 531, 1333
BmsI GCATC 2 cut(s) 635, 662
BpiI GAAGAC 2 cut(s) 254, 1080
Bpu1102I GCTNAGC 1 cut(s) 852
BpuEI CTTGAG 3 cut(s) 252, 564, 1517
BsaAI YACGTR 2 cut(s) 29, 71
BsaBI GATNNNNATC 1 cut(s) 1179
BsaHI GRCGYC 1 cut(s) 1305
BsaI GGTCTC 1 cut(s) 450
BsaJI CCNNGG 1 cut(s) 1331
BsaWI WCCGGW 2 cut(s) 11, 1171
Bsc4I CCNNNNNNNGG 1 cut(s) 258
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 2 cut(s) 185, 290
Bse21I CCTNAGG 1 cut(s) 684
Bse3DI GCAATG 1 cut(s) 621
Bse8I GATNNNNATC 1 cut(s) 1179
BseAI TCCGGA 1 cut(s) 1171
BseBI CCWGG 2 cut(s) 531, 1333
BseDI CCNNGG 1 cut(s) 1331
BseJI GATNNNNATC 1 cut(s) 1179
BseLI CCNNNNNNNGG 1 cut(s) 258
BseMI GCAATG 1 cut(s) 621
BseMII CTCAG 5 cut(s) 372, 698, 866, 1001, 1032
BseNI ACTGG 2 cut(s) 185, 290
BsePI GCGCGC 1 cut(s) 537
BseXI GCAGC 5 cut(s) 855, 1330, 1333, 1446, 1582
BseYI CCCAGC 1 cut(s) 930
Bsh1236I CGCG 1 cut(s) 539
BshFI GGCC 1 cut(s) 546
BshTI ACCGGT 1 cut(s) 11
BsiHKAI GWGCWC 2 cut(s) 150, 387
BsiHKCI CYCGRG 1 cut(s) 324
BsiSI CCGG 2 cut(s) 12, 1172
BslI CCNNNNNNNGG 1 cut(s) 258
BsmAI GTCTC 6 cut(s) 327, 450, 805, 820, 1037, 1495
BsmBI CGTCTC 1 cut(s) 1495
BsnI GGCC 1 cut(s) 546
Bso31I GGTCTC 1 cut(s) 450
BsoBI CYCGRG 1 cut(s) 324
Bsp1286I GDGCHC 2 cut(s) 150, 387
Bsp13I TCCGGA 1 cut(s) 1171
Bsp143I GATC 5 cut(s) 160, 286, 480, 1471, 1564
Bsp1720I GCTNAGC 1 cut(s) 852
BspACI CCGC 4 cut(s) 124, 182, 478, 950
BspANI GGCC 1 cut(s) 546
BspCNI CTCAG 5 cut(s) 373, 697, 865, 1000, 1033
BspEI TCCGGA 1 cut(s) 1171
BspFNI CGCG 1 cut(s) 539
BspHI TCATGA 1 cut(s) 1405
BspLI GGNNCC 3 cut(s) 288, 690, 1080
BspMAI CTGCAG 1 cut(s) 319
BspMI ACCTGC 1 cut(s) 883
BspPI GGATC 3 cut(s) 281, 294, 488
BspTNI GGTCTC 1 cut(s) 450
BsrDI GCAATG 1 cut(s) 621
BsrFI RCCGGY 1 cut(s) 11
BsrI ACTGG 2 cut(s) 185, 290
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 1 cut(s) 1331
BssHII GCGCGC 1 cut(s) 537
BssMI GATC 5 cut(s) 160, 286, 480, 1471, 1564
BssNAI GTATAC 1 cut(s) 74
BssNI GRCGYC 1 cut(s) 1305
BssSI CACGAG 1 cut(s) 386
Bst1107I GTATAC 1 cut(s) 74
Bst2BI CACGAG 1 cut(s) 386
Bst2UI CCWGG 2 cut(s) 531, 1333
Bst4CI ACNGT 5 cut(s) 154, 670, 1110, 1368, 1603
BstACI GRCGYC 1 cut(s) 1305
BstBAI YACGTR 2 cut(s) 29, 71
BstC8I GCNNGC 4 cut(s) 264, 539, 1233, 1575
BstDEI CTNAG 6 cut(s) 381, 649, 684, 852, 987, 1041
BstEII GGTNACC 1 cut(s) 1335
BstFNI CGCG 1 cut(s) 539
BstHHI GCGC 3 cut(s) 539, 541, 1035
BstKTI GATC 5 cut(s) 163, 289, 483, 1474, 1567
BstMAI GTCTC 6 cut(s) 327, 450, 805, 820, 1037, 1495
BstMBI GATC 5 cut(s) 160, 286, 480, 1471, 1564
BstMWI GCNNNNNNNGC 5 cut(s) 51, 543, 898, 946, 1327
BstNI CCWGG 2 cut(s) 531, 1333
BstNSI RCATGY 1 cut(s) 1050
BstPI GGTNACC 1 cut(s) 1335
BstSCI CCNGG 2 cut(s) 529, 1331
BstSFI CTRYAG 2 cut(s) 315, 944
BstUI CGCG 1 cut(s) 539
BstV1I GCAGC 5 cut(s) 855, 1330, 1333, 1446, 1582
BstV2I GAAGAC 2 cut(s) 254, 1080
BstX2I RGATCY 1 cut(s) 286
BstXI CCANNNNNNTGG 1 cut(s) 297
BstYI RGATCY 1 cut(s) 286
BstZ17I GTATAC 1 cut(s) 74
Bsu36I CCTNAGG 1 cut(s) 684
BsuRI GGCC 1 cut(s) 546
BtrI CACGTC 1 cut(s) 776
BtsI GCAGTG 1 cut(s) 599
BtsIMutI CAGTG 3 cut(s) 599, 666, 679
BveI ACCTGC 1 cut(s) 883
Cac8I GCNNGC 4 cut(s) 264, 539, 1233, 1575
CciI TCATGA 1 cut(s) 1405
CfoI GCGC 3 cut(s) 539, 541, 1035
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 1078
CseI GACGC 1 cut(s) 1294
Csp6I GTAC 7 cut(s) 30, 107, 354, 758, 1358, 1478, 1599
CspAI ACCGGT 1 cut(s) 11
CviAII CATG 6 cut(s) 587, 863, 1030, 1047, 1406, 1616
CviQI GTAC 7 cut(s) 30, 107, 354, 758, 1358, 1478, 1599
DdeI CTNAG 6 cut(s) 381, 649, 684, 852, 987, 1041
DpnI GATC 5 cut(s) 162, 288, 482, 1473, 1566
DpnII GATC 5 cut(s) 160, 286, 480, 1471, 1564
DriI GACNNNNNGTC 1 cut(s) 1084
Eam1105I GACNNNNNGTC 1 cut(s) 1084
Eco31I GGTCTC 1 cut(s) 450
Eco47I GGWCC 1 cut(s) 1078
Eco81I CCTNAGG 1 cut(s) 684
Eco88I CYCGRG 1 cut(s) 324
Eco91I GGTNACC 1 cut(s) 1335
EcoO109I RGGNCCY 1 cut(s) 1078
EcoO65I GGTNACC 1 cut(s) 1335
EcoRII CCWGG 2 cut(s) 529, 1331
Esp3I CGTCTC 1 cut(s) 1495
FaeI CATG 6 cut(s) 590, 866, 1033, 1050, 1409, 1619
FatI CATG 6 cut(s) 586, 862, 1029, 1046, 1405, 1615
FauI CCCGC 1 cut(s) 117
FauNDI CATATG 2 cut(s) 396, 922
FbaI TGATCA 1 cut(s) 160
FblI GTMKAC 1 cut(s) 73
Fnu4HI GCNGC 7 cut(s) 182, 869, 950, 1319, 1322, 1460, 1571
Fsp4HI GCNGC 7 cut(s) 182, 869, 950, 1319, 1322, 1460, 1571
FspBI CTAG 2 cut(s) 606, 708
GlaI GCGC 3 cut(s) 538, 540, 1034
GluI GCNGC 7 cut(s) 182, 869, 950, 1319, 1322, 1460, 1571
GsaI CCCAGC 1 cut(s) 934
HaeIII GGCC 1 cut(s) 546
HapII CCGG 2 cut(s) 12, 1172
HgaI GACGC 1 cut(s) 1294
HhaI GCGC 3 cut(s) 539, 541, 1035
Hin1I GRCGYC 1 cut(s) 1305
Hin1II CATG 6 cut(s) 590, 866, 1033, 1050, 1409, 1619
Hin6I GCGC 3 cut(s) 537, 539, 1033
HinP1I GCGC 3 cut(s) 537, 539, 1033
HincII GTYRAC 1 cut(s) 1257
HindII GTYRAC 1 cut(s) 1257
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 7 cut(s) 137, 320, 506, 526, 1123, 1168, 1612
HpaII CCGG 2 cut(s) 12, 1172
HphI GGTGA 4 cut(s) 26, 828, 906, 1329
Hpy166II GTNNAC 6 cut(s) 32, 74, 109, 340, 1202, 1257
Hpy188I TCNGA 6 cut(s) 210, 382, 403, 1042, 1066, 1101
Hpy188III TCNNGA 8 cut(s) 324, 326, 453, 614, 771, 1172, 1406, 1496
Hpy8I GTNNAC 6 cut(s) 32, 74, 109, 340, 1202, 1257
HpyAV CCTTC 1 cut(s) 1047
HpyCH4III ACNGT 5 cut(s) 154, 670, 1110, 1368, 1603
HpyCH4IV ACGT 5 cut(s) 28, 70, 238, 775, 1356
HpyF10VI GCNNNNNNNGC 5 cut(s) 51, 543, 898, 946, 1327
HpyF3I CTNAG 6 cut(s) 381, 649, 684, 852, 987, 1041
HpySE526I ACGT 5 cut(s) 28, 70, 238, 775, 1356
Hsp92I GRCGYC 1 cut(s) 1305
Hsp92II CATG 6 cut(s) 590, 866, 1033, 1050, 1409, 1619
HspAI GCGC 3 cut(s) 537, 539, 1033
Kpn2I TCCGGA 1 cut(s) 1171
Ksp22I TGATCA 1 cut(s) 160
Kzo9I GATC 5 cut(s) 160, 286, 480, 1471, 1564
LmnI GCTCC 2 cut(s) 577, 817
Lsp1109I GCAGC 5 cut(s) 855, 1330, 1333, 1446, 1582
LweI GCATC 2 cut(s) 635, 662
MaeI CTAG 2 cut(s) 606, 708
MaeII ACGT 5 cut(s) 28, 70, 238, 775, 1356
MaeIII GTNAC 8 cut(s) 14, 676, 925, 1128, 1165, 1280, 1335, 1374
MalI GATC 5 cut(s) 162, 288, 482, 1473, 1566
MboI GATC 5 cut(s) 160, 286, 480, 1471, 1564
MboII GAAGA 5 cut(s) 259, 501, 509, 1080, 1543
MfeI CAATTG 3 cut(s) 295, 1412, 1541
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 2 cut(s) 150, 387
MlyI GAGTC 3 cut(s) 146, 329, 1162
MmeI TCCRAC 2 cut(s) 474, 1369
MroI TCCGGA 1 cut(s) 1171
MseI TTAA 5 cut(s) 359, 977, 1119, 1214, 1311
MslI CAYNNNNRTG 2 cut(s) 1185, 1287
MspA1I CMGCKG 1 cut(s) 1459
MspI CCGG 2 cut(s) 12, 1172
MspR9I CCNGG 2 cut(s) 531, 1333
MunI CAATTG 3 cut(s) 295, 1412, 1541
MvaI CCWGG 2 cut(s) 531, 1333
MvnI CGCG 1 cut(s) 539
MwoI GCNNNNNNNGC 5 cut(s) 51, 543, 898, 946, 1327
NdeI CATATG 2 cut(s) 396, 922
NdeII GATC 5 cut(s) 160, 286, 480, 1471, 1564
NlaIII CATG 6 cut(s) 590, 866, 1033, 1050, 1409, 1619
NlaIV GGNNCC 3 cut(s) 288, 690, 1080
NmuCI GTSAC 5 cut(s) 14, 1128, 1165, 1280, 1335
NspI RCATGY 1 cut(s) 1050
PaeR7I CTCGAG 1 cut(s) 324
PagI TCATGA 1 cut(s) 1405
PauI GCGCGC 1 cut(s) 537
PciI ACATGT 1 cut(s) 1046
PfeI GAWTC 4 cut(s) 506, 526, 1123, 1612
PinAI ACCGGT 1 cut(s) 11
PkrI GCNGC 7 cut(s) 183, 870, 951, 1320, 1323, 1461, 1572
PleI GAGTC 3 cut(s) 145, 328, 1162
PpsI GAGTC 3 cut(s) 145, 328, 1162
Ppu21I YACGTR 2 cut(s) 29, 71
PpuMI RGGWCCY 1 cut(s) 1078
PscI ACATGT 1 cut(s) 1046
PshBI ATTAAT 1 cut(s) 1214
PsiI TTATAA 1 cut(s) 789
Psp5II RGGWCCY 1 cut(s) 1078
Psp6I CCWGG 2 cut(s) 529, 1331
PspEI GGTNACC 1 cut(s) 1335
PspFI CCCAGC 1 cut(s) 930
PspGI CCWGG 2 cut(s) 529, 1331
PspN4I GGNNCC 3 cut(s) 288, 690, 1080
PspPI GGNCC 1 cut(s) 1078
PspPPI RGGWCCY 1 cut(s) 1078
PstI CTGCAG 1 cut(s) 319
PsuI RGATCY 1 cut(s) 286
PteI GCGCGC 1 cut(s) 537
PvuII CAGCTG 1 cut(s) 1459
RsaI GTAC 7 cut(s) 31, 108, 355, 759, 1359, 1479, 1600
RsaNI GTAC 7 cut(s) 30, 107, 354, 758, 1358, 1478, 1599
RseI CAYNNNNRTG 2 cut(s) 1185, 1287
SaqAI TTAA 5 cut(s) 359, 977, 1119, 1214, 1311
SatI GCNGC 7 cut(s) 182, 869, 950, 1319, 1322, 1460, 1571
Sau3AI GATC 5 cut(s) 160, 286, 480, 1471, 1564
Sau96I GGNCC 1 cut(s) 1078
ScaI AGTACT 1 cut(s) 1600
SchI GAGTC 3 cut(s) 146, 329, 1162
ScrFI CCNGG 2 cut(s) 531, 1333
SduI GDGCHC 2 cut(s) 150, 387
SfaNI GCATC 2 cut(s) 635, 662
SfcI CTRYAG 2 cut(s) 315, 944
Sfr274I CTCGAG 1 cut(s) 324
SinI GGWCC 1 cut(s) 1078
SlaI CTCGAG 1 cut(s) 324
SmiMI CAYNNNNRTG 2 cut(s) 1185, 1287
SmlI CTYRAG 4 cut(s) 267, 324, 579, 1496
SmoI CTYRAG 4 cut(s) 267, 324, 579, 1496
SsiI CCGC 4 cut(s) 124, 182, 478, 950
SspI AATATT 2 cut(s) 793, 1432
SspMI CTAG 2 cut(s) 606, 708
StyD4I CCNGG 2 cut(s) 529, 1331
TaaI ACNGT 5 cut(s) 154, 670, 1110, 1368, 1603
TaiI ACGT 5 cut(s) 31, 73, 241, 778, 1359
TaqI TCGA 4 cut(s) 156, 325, 429, 613
TatI WGTACW 1 cut(s) 1598
TauI GCSGC 2 cut(s) 184, 952
TfiI GAWTC 4 cut(s) 506, 526, 1123, 1612
Tru1I TTAA 5 cut(s) 359, 977, 1119, 1214, 1311
Tru9I TTAA 5 cut(s) 359, 977, 1119, 1214, 1311
TscAI CASTG 3 cut(s) 606, 673, 679
TseFI GTSAC 5 cut(s) 14, 1128, 1165, 1280, 1335
TseI GCWGC 5 cut(s) 868, 1318, 1321, 1459, 1570
Tsp45I GTSAC 5 cut(s) 14, 1128, 1165, 1280, 1335
TspDTI ATGAA 4 cut(s) 377, 603, 1169, 1550
TspRI CASTG 3 cut(s) 606, 673, 679
VpaK11BI GGWCC 1 cut(s) 1078
VspI ATTAAT 1 cut(s) 1214
XapI RAATTY 5 cut(s) 308, 424, 715, 763, 832
XceI RCATGY 1 cut(s) 1050
XhoI CTCGAG 1 cut(s) 324
XmiI GTMKAC 1 cut(s) 73
XspI CTAG 2 cut(s) 606, 708
ZrmI AGTACT 1 cut(s) 1600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.