Rh1BG077900

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001760
Physical Location & Seq
Reverse (-)
12115837 .. 12117421
1585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG077900.1

Sequence Viewer

Length: 1455 bp
ATGAAAGTGGAAAACCAGAGCTCCAGTCCTTTACCCTTTTGGTCTAGGAATCGGCAAATCCATTATTATTTAGGAAACCCTGTAAAAGAAAAAACTATTTATATTGCAGCAGGAAAACCAATTTCTGCAATCCCACCACCAGCAGAAACTCTCAATAATCTGTCATGCATGCAGCTTCCATTTTCTGATCTTCAGCAGAATCATCAGATGTCTCCTTTTCAGCAGACATCACAATCTGCGATTAACAATCATCCACAGACAGGCTTTAGGCAAATAAACTCATCACAACATGCTCCTTATATTCATCAGAATGCATCATCAATTCCACAGAGTCTGCTAATGCAATCGCAGAAGCATGTTGTGATGAGGAGAAGATTAGCTGCCACCACTCTACAACAGAATCAGTCAACTTGCCTCGACTTTCCGCAGCAACAACACATTAACACATACCAGCTGAATGATGCACAACAGCAGTTACATAGACAGAACCTTAATCTTTCGGGTCCGCATCATGAGCAATTGAGCCAAAGAAACATTATCTCTATACAGCAACAAACTCTTGAGAGACAGTTGAATTCTTCACAGGTAGAGAAGATGCAAAGGCAGCAAAACCGAGTAGAACAAGATGAGCAGCAAACACATCAAATGGCAGGAATGCCAGCTTCCACATTATTAATGGAAGGGGAGGTCTACCGAAAGGTCCAAATGCTAAAACGCAAGTATGAAAGACCACTGAAAGTTATCTTCCAAAGGTTCACATATTGTCTCCAGAACCTAGATAGACACAGTATCAACAAACTTGAAGTAGTAAACAGCTTGTATCAGACTGTCAAGTCTATCCTTAGGATGCTGAATGTGCCAGAAAGAGACACTATGCCTACTACCAAGGAGAAGTTGGGTCTACTTGAGAAGAAAATCGAAGACATTCTCCACCCATGTGGAAACCAACAGCGTCAATTGGTGCAAATTATTGATTCTCGGACTCTTCAAGCTCAGACAAACCAAGAGGAAAAGAAGCCACCAACGCAGACAGTGAATATAGAATGTTTTGCAACAAAGATGCAGCAGAGCAATGTGACAAACTTGATGATGAACGATTCTCAGCCTCTGATCGAGAAACATGGCAGAAAACATTTGCCAAGAAACTTGACTAGGAAAGATTCAGATCAAGCAAGTTCGTCCGGGTACTTGACAATGCCTACAAGTAGTCCCCAACAACAAAATGTTATGACCTTGTTGTCACAAAACCCCATTCAGTCAAATGTCAATAATTTGGAGTCAAGATTTAGTGTACCTCGATATGTGCACCTGACGCAATCTAAAGAACAGCAGGATGTGCAAACTCAAAAACCAAAACAGCAATTACTGCAGGAGAATCACCGGCTGGTGAAGTTGCAGTCTGAAGCTTTTCCTAATCCTTTGCGGGAAGATCATAATTCTTCAACAAGTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

484

Amino Acids

55.96

Weight (kDa)

9.57

Isoelectric Point (pI)

71.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 832, 1237
AccI GTMKAC 2 cut(s) 690, 901
AciI CCGC 3 cut(s) 425, 506, 1423
AcsI RAATTY 1 cut(s) 574
AcuI CTGAAG 2 cut(s) 176, 1422
AfaI GTAC 2 cut(s) 1187, 1293
AfiI CCNNNNNNNGG 1 cut(s) 260
AgsI TTSAA 4 cut(s) 574, 803, 989, 1443
AleI CACNNNNGTG 1 cut(s) 936
AluBI AGCT 8 cut(s) 21, 175, 380, 454, 662, 816, 992, 1406
AluI AGCT 8 cut(s) 21, 175, 380, 454, 662, 816, 992, 1406
Alw21I GWGCWC 2 cut(s) 23, 1308
Alw26I GTCTC 4 cut(s) 216, 559, 770, 861
Alw44I GTGCAC 1 cut(s) 1304
AlwNI CAGNNNCTG 2 cut(s) 334, 1108
ApaLI GTGCAC 1 cut(s) 1304
ApeKI GCWGC 7 cut(s) 107, 172, 380, 427, 604, 631, 1063
ApoI RAATTY 1 cut(s) 574
AseI ATTAAT 1 cut(s) 674
Asp700I GAANNNNTTC 2 cut(s) 924, 1407
AspS9I GGNCC 2 cut(s) 503, 700
AsuC2I CCSGG 1 cut(s) 1183
AsuHPI GGTGA 2 cut(s) 1370, 1399
AvaII GGWCC 2 cut(s) 503, 700
AxyI CCTNAGG 1 cut(s) 842
BaeGI GKGCMC 1 cut(s) 1308
BanII GRGCYC 1 cut(s) 23
BbsI GAAGAC 1 cut(s) 927
Bbv12I GWGCWC 2 cut(s) 23, 1308
BbvI GCAGC 7 cut(s) 119, 184, 367, 439, 616, 643, 1075
BcnI CCSGG 1 cut(s) 1183
BcoDI GTCTC 4 cut(s) 216, 559, 770, 861
BfaI CTAG 3 cut(s) 45, 776, 1152
BfmI CTRYAG 1 cut(s) 1367
BisI GCNGC 7 cut(s) 108, 173, 381, 428, 605, 632, 1064
BlsI GCNGC 7 cut(s) 109, 174, 382, 429, 606, 633, 1065
Bme1390I CCNGG 1 cut(s) 1183
Bme18I GGWCC 2 cut(s) 503, 700
BmgT120I GGNCC 2 cut(s) 503, 700
BmiI GGNNCC 1 cut(s) 504
BmrFI CCNGG 1 cut(s) 1183
BmsI GCATC 6 cut(s) 323, 451, 517, 585, 837, 1050
BpiI GAAGAC 1 cut(s) 927
BpmI CTGGAG 2 cut(s) 7, 752
BpuEI CTTGAG 2 cut(s) 581, 926
BpuMI CCSGG 1 cut(s) 1183
BsaBI GATNNNNATC 1 cut(s) 1164
BsaJI CCNNGG 1 cut(s) 885
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 1 cut(s) 260
Bse118I RCCGGY 1 cut(s) 1380
Bse1I ACTGG 1 cut(s) 24
Bse21I CCTNAGG 1 cut(s) 842
Bse3DI GCAATG 1 cut(s) 1078
Bse8I GATNNNNATC 1 cut(s) 1164
BseDI CCNNGG 1 cut(s) 885
BseGI GGATG 3 cut(s) 250, 852, 1339
BseJI GATNNNNATC 1 cut(s) 1164
BseLI CCNNNNNNNGG 1 cut(s) 260
BseMI GCAATG 1 cut(s) 1078
BseMII CTCAG 2 cut(s) 1007, 1115
BseNI ACTGG 1 cut(s) 24
BseRI GAGGAG 1 cut(s) 382
BseSI GKGCMC 1 cut(s) 1308
BseXI GCAGC 7 cut(s) 119, 184, 367, 439, 616, 643, 1075
BsiHKAI GWGCWC 2 cut(s) 23, 1308
BsiSI CCGG 2 cut(s) 1182, 1381
BslFI GGGAC 1 cut(s) 1194
BslI CCNNNNNNNGG 1 cut(s) 260
BsmAI GTCTC 4 cut(s) 216, 559, 770, 861
BsmFI GGGAC 1 cut(s) 1194
BsmI GAATGC 2 cut(s) 316, 660
Bsp1286I GDGCHC 2 cut(s) 23, 1308
Bsp143I GATC 4 cut(s) 187, 1110, 1165, 1429
BspACI CCGC 3 cut(s) 425, 506, 1423
BspCNI CTCAG 2 cut(s) 1006, 1114
BspHI TCATGA 1 cut(s) 511
BspLI GGNNCC 1 cut(s) 504
BspMAI CTGCAG 1 cut(s) 1371
BsrDI GCAATG 1 cut(s) 1078
BsrFI RCCGGY 1 cut(s) 1380
BsrI ACTGG 1 cut(s) 24
BssAI RCCGGY 1 cut(s) 1380
BssECI CCNNGG 1 cut(s) 885
BssMI GATC 4 cut(s) 187, 1110, 1165, 1429
BssT1I CCWWGG 1 cut(s) 885
Bst4CI ACNGT 4 cut(s) 570, 788, 829, 1033
Bst6I CTCTTC 1 cut(s) 990
BstAPI GCANNNNNTGC 2 cut(s) 1336, 1366
BstC8I GCNNGC 2 cut(s) 170, 660
BstDEI CTNAG 3 cut(s) 842, 993, 1101
BstF5I GGATG 3 cut(s) 250, 852, 1339
BstKTI GATC 4 cut(s) 190, 1113, 1168, 1432
BstMAI GTCTC 4 cut(s) 216, 559, 770, 861
BstMBI GATC 4 cut(s) 187, 1110, 1165, 1429
BstMWI GCNNNNNNNGC 7 cut(s) 514, 604, 856, 1024, 1312, 1336, 1366
BstNSI RCATGY 3 cut(s) 172, 293, 359
BstSCI CCNGG 1 cut(s) 1181
BstSFI CTRYAG 1 cut(s) 1367
BstSLI GKGCMC 1 cut(s) 1308
BstV1I GCAGC 7 cut(s) 119, 184, 367, 439, 616, 643, 1075
BstV2I GAAGAC 1 cut(s) 927
BstXI CCANNNNNNTGG 1 cut(s) 938
Bsu36I CCTNAGG 1 cut(s) 842
BtsCI GGATG 3 cut(s) 250, 852, 1339
BtsIMutI CAGTG 2 cut(s) 731, 1038
Cac8I GCNNGC 2 cut(s) 170, 660
CaiI CAGNNNCTG 2 cut(s) 334, 1108
CciI TCATGA 1 cut(s) 511
Cfr10I RCCGGY 1 cut(s) 1380
Cfr13I GGNCC 2 cut(s) 503, 700
CseI GACGC 2 cut(s) 941, 1321
Csp6I GTAC 2 cut(s) 1186, 1292
CviAII CATG 7 cut(s) 165, 169, 290, 356, 512, 936, 1121
CviQI GTAC 2 cut(s) 1186, 1292
DdeI CTNAG 3 cut(s) 842, 993, 1101
DpnI GATC 4 cut(s) 189, 1112, 1167, 1431
DpnII GATC 4 cut(s) 187, 1110, 1165, 1429
DrdI GACNNNNNNGTC 2 cut(s) 832, 1237
DseDI GACNNNNNNGTC 2 cut(s) 832, 1237
Eam1104I CTCTTC 1 cut(s) 990
EarI CTCTTC 1 cut(s) 990
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 885
Eco24I GRGCYC 1 cut(s) 23
Eco47I GGWCC 2 cut(s) 503, 700
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 2 cut(s) 176, 1422
Eco81I CCTNAGG 1 cut(s) 842
EcoICRI GAGCTC 1 cut(s) 21
EcoRI GAATTC 1 cut(s) 574
EcoT14I CCWWGG 1 cut(s) 885
EcoT22I ATGCAT 2 cut(s) 170, 316
EcoT38I GRGCYC 1 cut(s) 23
ErhI CCWWGG 1 cut(s) 885
FaeI CATG 7 cut(s) 168, 172, 293, 359, 515, 939, 1124
FaqI GGGAC 1 cut(s) 1194
FatI CATG 7 cut(s) 164, 168, 289, 355, 511, 935, 1120
FauI CCCGC 1 cut(s) 1416
FblI GTMKAC 2 cut(s) 690, 901
Fnu4HI GCNGC 7 cut(s) 108, 173, 381, 428, 605, 632, 1064
FokI GGATG 3 cut(s) 237, 859, 1346
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 7 cut(s) 108, 173, 381, 428, 605, 632, 1064
FspBI CTAG 3 cut(s) 45, 776, 1152
GluI GCNGC 7 cut(s) 108, 173, 381, 428, 605, 632, 1064
GsuI CTGGAG 2 cut(s) 7, 752
HapII CCGG 2 cut(s) 1182, 1381
HgaI GACGC 2 cut(s) 941, 1321
Hin1II CATG 7 cut(s) 168, 172, 293, 359, 515, 939, 1124
HincII GTYRAC 1 cut(s) 408
HindII GTYRAC 1 cut(s) 408
HindIII AAGCTT 1 cut(s) 1404
HpaII CCGG 2 cut(s) 1182, 1381
HphI GGTGA 2 cut(s) 1370, 1399
Hpy166II GTNNAC 7 cut(s) 408, 691, 756, 811, 902, 1292, 1306
Hpy188I TCNGA 9 cut(s) 187, 207, 309, 825, 981, 996, 1110, 1165, 1402
Hpy188III TCNNGA 5 cut(s) 512, 560, 769, 1114, 1281
Hpy8I GTNNAC 7 cut(s) 408, 691, 756, 811, 902, 1292, 1306
HpyAV CCTTC 1 cut(s) 674
HpyCH4III ACNGT 4 cut(s) 570, 788, 829, 1033
HpyF10VI GCNNNNNNNGC 7 cut(s) 514, 604, 856, 1024, 1312, 1336, 1366
HpyF3I CTNAG 3 cut(s) 842, 993, 1101
Hsp92II CATG 7 cut(s) 168, 172, 293, 359, 515, 939, 1124
Kzo9I GATC 4 cut(s) 187, 1110, 1165, 1429
LmnI GCTCC 2 cut(s) 26, 298
Lsp1109I GCAGC 7 cut(s) 119, 184, 367, 439, 616, 643, 1075
LweI GCATC 6 cut(s) 323, 451, 517, 585, 837, 1050
MaeI CTAG 3 cut(s) 45, 776, 1152
MaeIII GTNAC 3 cut(s) 474, 1075, 1239
MalI GATC 4 cut(s) 189, 1112, 1167, 1431
MboI GATC 4 cut(s) 187, 1110, 1165, 1429
MfeI CAATTG 2 cut(s) 518, 956
MhlI GDGCHC 2 cut(s) 23, 1308
MluCI AATT 9 cut(s) 120, 321, 518, 574, 956, 966, 1270, 1361, 1435
MlyI GAGTC 3 cut(s) 340, 976, 1286
MnlI CCTC 6 cut(s) 360, 425, 679, 1000, 1116, 1305
Mph1103I ATGCAT 2 cut(s) 170, 316
MroXI GAANNNNTTC 2 cut(s) 924, 1407
MseI TTAA 4 cut(s) 243, 441, 492, 674
MslI CAYNNNNRTG 2 cut(s) 309, 936
MspA1I CMGCKG 1 cut(s) 454
MspI CCGG 2 cut(s) 1182, 1381
MspR9I CCNGG 1 cut(s) 1183
MunI CAATTG 2 cut(s) 518, 956
Mva1269I GAATGC 2 cut(s) 316, 660
MwoI GCNNNNNNNGC 7 cut(s) 514, 604, 856, 1024, 1312, 1336, 1366
NciI CCSGG 1 cut(s) 1183
NdeII GATC 4 cut(s) 187, 1110, 1165, 1429
NlaIII CATG 7 cut(s) 168, 172, 293, 359, 515, 939, 1124
NlaIV GGNNCC 1 cut(s) 504
NmuCI GTSAC 2 cut(s) 1075, 1239
NsiI ATGCAT 2 cut(s) 170, 316
NspI RCATGY 3 cut(s) 172, 293, 359
OliI CACNNNNGTG 1 cut(s) 936
PaeI GCATGC 1 cut(s) 172
PagI TCATGA 1 cut(s) 511
PctI GAATGC 2 cut(s) 316, 660
PdmI GAANNNNTTC 2 cut(s) 924, 1407
PfeI GAWTC 7 cut(s) 49, 199, 400, 974, 1097, 1160, 1375
PkrI GCNGC 7 cut(s) 109, 174, 382, 429, 606, 633, 1065
PleI GAGTC 3 cut(s) 339, 976, 1285
PpsI GAGTC 3 cut(s) 339, 976, 1285
PshBI ATTAAT 1 cut(s) 674
Psp124BI GAGCTC 1 cut(s) 23
PspN4I GGNNCC 1 cut(s) 504
PspPI GGNCC 2 cut(s) 503, 700
PstI CTGCAG 1 cut(s) 1371
PstNI CAGNNNCTG 2 cut(s) 334, 1108
PvuII CAGCTG 1 cut(s) 454
RsaI GTAC 2 cut(s) 1187, 1293
RsaNI GTAC 2 cut(s) 1186, 1292
RseI CAYNNNNRTG 2 cut(s) 309, 936
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 4 cut(s) 243, 441, 492, 674
SatI GCNGC 7 cut(s) 108, 173, 381, 428, 605, 632, 1064
Sau3AI GATC 4 cut(s) 187, 1110, 1165, 1429
Sau96I GGNCC 2 cut(s) 503, 700
SchI GAGTC 3 cut(s) 340, 976, 1286
ScrFI CCNGG 1 cut(s) 1183
SduI GDGCHC 2 cut(s) 23, 1308
SfaNI GCATC 6 cut(s) 323, 451, 517, 585, 837, 1050
SfcI CTRYAG 1 cut(s) 1367
SinI GGWCC 2 cut(s) 503, 700
SmiMI CAYNNNNRTG 2 cut(s) 309, 936
SmlI CTYRAG 2 cut(s) 560, 905
SmoI CTYRAG 2 cut(s) 560, 905
SphI GCATGC 1 cut(s) 172
Sse9I AATT 9 cut(s) 120, 321, 518, 574, 956, 966, 1270, 1361, 1435
SsiI CCGC 3 cut(s) 425, 506, 1423
SspMI CTAG 3 cut(s) 45, 776, 1152
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 1181
StyI CCWWGG 1 cut(s) 885
TaaI ACNGT 4 cut(s) 570, 788, 829, 1033
TaqI TCGA 4 cut(s) 417, 918, 1113, 1297
TasI AATT 9 cut(s) 120, 321, 518, 574, 956, 966, 1270, 1361, 1435
TfiI GAWTC 7 cut(s) 49, 199, 400, 974, 1097, 1160, 1375
Tru1I TTAA 4 cut(s) 243, 441, 492, 674
Tru9I TTAA 4 cut(s) 243, 441, 492, 674
TscAI CASTG 2 cut(s) 738, 1038
TseFI GTSAC 2 cut(s) 1075, 1239
TseI GCWGC 7 cut(s) 107, 172, 380, 427, 604, 631, 1063
Tsp45I GTSAC 2 cut(s) 1075, 1239
TspDTI ATGAA 4 cut(s) 17, 293, 738, 1106
TspRI CASTG 2 cut(s) 738, 1038
VneI GTGCAC 1 cut(s) 1304
VpaK11BI GGWCC 2 cut(s) 503, 700
VspI ATTAAT 1 cut(s) 674
XapI RAATTY 1 cut(s) 574
XceI RCATGY 3 cut(s) 172, 293, 359
XcmI CCANNNNNNNNNTGG 2 cut(s) 673, 892
XmiI GTMKAC 2 cut(s) 690, 901
XmnI GAANNNNTTC 2 cut(s) 924, 1407
XspI CTAG 3 cut(s) 45, 776, 1152
Zsp2I ATGCAT 2 cut(s) 170, 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.