Rh1DG058200

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0001760
Physical Location & Seq
Reverse (-)
9702302 .. 9705765
3464 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG058200.1

Sequence Viewer

Length: 1017 bp
ATGCTGGGACAAGCAGAGCTAAGTGCTTTTGCTTCAGACATTGGTTCAGTAGTGTGTATAACTGATAGAATGTCTGGACCAGATAATGTTCAGAGATCTAGAGCTGTGATTGGTGATTTGGCAAACATGACCAATTTTCATTTGCAGGAGAGATACTTTGCCTGGCAGGAAACAACTGTTGGAACAAGGATAATGAAGCGTCATAGAAGTATGCCAATTAGCTATGGGGCTTCAGAAAATAGTAGTATAAATGATAGCTTGTCACAGCTAAGTGATATGGAAAATTTAGACTTGGACTCACCTGCAATATCTTATAGCAAACGGCGTAGGCTTGAGGTGAATCATCTCTTAGAAGAAATTACCGCTATCAACCAAGAACTAATAGACACTGTTTTAGATATTAGAGATGGAGAGACTATTCCATCAGTAGCCTCATCTCCAATTCTCGATGGTGAAGGAACCATTGTTAGGTGTTCCTTCATTGCTATAGTCGTTGCTCCTAATCTGAAGTCTGAATGTGTTCCAGCTCCAATGCTTGCAATCCAGCCTCTGCGATTGCTTGTTCCTGCAAATTATCCTTTGTGCTCTCCTATATTTATGGACAAGTTGAAAGTGGAAGTCAGATACTTCTCAACGCCTTACTCACTCCCCAAACTTGATACGGTTGCGGTTCCTGAATTTTCTGGTGGGCCCATGGAGAATTATGGTTTGATCACATACCTTGAAAATGGACTGCTTTTTGATCCTTTGCATTCCACAGCAGCAAGAAAGCAAAGAAAAAGTTATATGGCCACTAATATTTTGTTTCCTAAGTGTAAAGTTTGGTCTCAATTTCTTCAACAAACTACTGGTGTACTAATTATGGATGCGCTGGAACATTCACATCCAATTCAGGTAGAGGTACACCACGCACGTTCAGTTCTTGAAATATTCGATACTATCAGCTATAAAAAGGGATCTGTTGTTATACAAATGCTTCAGGCTTATCTTGGTGATGATATATTCCAGTCATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

338

Amino Acids

37.72

Weight (kDa)

5.41

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M1 PF01433 202 - 259 6.6e-13 Peptidase family M1 domain
Peptidase_M1 PF01433 262 - 337 3.8e-15 Peptidase family M1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 310
Acc36I ACCTGC 1 cut(s) 310
AciI CCGC 2 cut(s) 363, 668
AclWI GGATC 2 cut(s) 737, 964
AcoI YGGCCR 1 cut(s) 789
AcsI RAATTY 2 cut(s) 283, 677
AcuI CTGAAG 4 cut(s) 18, 216, 527, 962
AfaI GTAC 2 cut(s) 855, 903
AfiI CCNNNNNNNGG 1 cut(s) 468
AgsI TTSAA 4 cut(s) 610, 725, 839, 926
AjnI CCWGG 1 cut(s) 161
AjuI GAANNNNNNNTTGG 2 cut(s) 162, 194
AluBI AGCT 7 cut(s) 19, 104, 222, 258, 268, 527, 945
AluI AGCT 7 cut(s) 19, 104, 222, 258, 268, 527, 945
Alw21I GWGCWC 1 cut(s) 587
Alw26I GTCTC 2 cut(s) 407, 831
AlwI GGATC 2 cut(s) 737, 964
AlwNI CAGNNNCTG 1 cut(s) 550
AoxI GGCC 2 cut(s) 689, 789
ApaI GGGCCC 1 cut(s) 693
ApeKI GCWGC 1 cut(s) 761
ApoI RAATTY 2 cut(s) 283, 677
Asp700I GAANNNNTTC 1 cut(s) 519
AspLEI GCGC 1 cut(s) 871
AspS9I GGNCC 3 cut(s) 77, 689, 690
AsuHPI GGTGA 5 cut(s) 125, 291, 349, 464, 1004
AvaII GGWCC 1 cut(s) 77
BaeGI GKGCMC 1 cut(s) 693
BalI TGGCCA 1 cut(s) 791
BanII GRGCYC 1 cut(s) 693
Bbv12I GWGCWC 1 cut(s) 587
BbvI GCAGC 1 cut(s) 773
BccI CCATC 3 cut(s) 401, 430, 443
BceAI ACGGC 1 cut(s) 338
BciT130I CCWGG 1 cut(s) 163
BclI TGATCA 1 cut(s) 711
BcoDI GTCTC 2 cut(s) 407, 831
BfaI CTAG 2 cut(s) 99, 1015
BfmI CTRYAG 1 cut(s) 486
BfuAI ACCTGC 1 cut(s) 310
BglII AGATCT 1 cut(s) 95
BisI GCNGC 1 cut(s) 762
BlsI GCNGC 1 cut(s) 763
Bme1390I CCNGG 1 cut(s) 163
Bme18I GGWCC 1 cut(s) 77
BmgT120I GGNCC 3 cut(s) 77, 689, 690
BmiI GGNNCC 3 cut(s) 460, 672, 691
BmrFI CCNGG 1 cut(s) 163
BmsI GCATC 1 cut(s) 856
BpuEI CTTGAG 1 cut(s) 353
BsaI GGTCTC 1 cut(s) 831
BsaJI CCNNGG 1 cut(s) 693
Bsc4I CCNNNNNNNGG 1 cut(s) 468
Bse1I ACTGG 2 cut(s) 853, 1006
Bse3DI GCAATG 1 cut(s) 480
BseBI CCWGG 1 cut(s) 163
BseDI CCNNGG 1 cut(s) 693
BseGI GGATG 2 cut(s) 871, 883
BseLI CCNNNNNNNGG 1 cut(s) 468
BseMI GCAATG 1 cut(s) 480
BseNI ACTGG 2 cut(s) 853, 1006
BseSI GKGCMC 1 cut(s) 693
BseXI GCAGC 1 cut(s) 773
BseYI CCCAGC 1 cut(s) 4
BshFI GGCC 2 cut(s) 691, 791
BsiHKAI GWGCWC 1 cut(s) 587
BslFI GGGAC 1 cut(s) 21
BslI CCNNNNNNNGG 1 cut(s) 468
BsmAI GTCTC 2 cut(s) 407, 831
BsmFI GGGAC 1 cut(s) 21
BsmI GAATGC 1 cut(s) 751
BsnI GGCC 2 cut(s) 691, 791
Bso31I GGTCTC 1 cut(s) 831
Bsp120I GGGCCC 1 cut(s) 689
Bsp1286I GDGCHC 2 cut(s) 587, 693
Bsp143I GATC 4 cut(s) 95, 711, 742, 956
Bsp19I CCATGG 1 cut(s) 693
BspACI CCGC 2 cut(s) 363, 668
BspANI GGCC 2 cut(s) 691, 791
BspLI GGNNCC 3 cut(s) 460, 672, 691
BspMI ACCTGC 1 cut(s) 310
BspPI GGATC 2 cut(s) 737, 964
BspTNI GGTCTC 1 cut(s) 831
BsrDI GCAATG 1 cut(s) 480
BsrI ACTGG 2 cut(s) 853, 1006
BssECI CCNNGG 1 cut(s) 693
BssMI GATC 4 cut(s) 95, 711, 742, 956
BssT1I CCWWGG 1 cut(s) 693
Bst2UI CCWGG 1 cut(s) 163
Bst4CI ACNGT 3 cut(s) 178, 391, 664
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 4 cut(s) 20, 269, 349, 810
BstDSI CCRYGG 1 cut(s) 693
BstF5I GGATG 2 cut(s) 871, 883
BstHHI GCGC 1 cut(s) 871
BstKTI GATC 4 cut(s) 98, 714, 745, 959
BstMAI GTCTC 2 cut(s) 407, 831
BstMBI GATC 4 cut(s) 95, 711, 742, 956
BstNI CCWGG 1 cut(s) 163
BstSCI CCNGG 1 cut(s) 161
BstSFI CTRYAG 1 cut(s) 486
BstSLI GKGCMC 1 cut(s) 693
BstV1I GCAGC 1 cut(s) 773
BstX2I RGATCY 2 cut(s) 95, 956
BstYI RGATCY 2 cut(s) 95, 956
BsuRI GGCC 2 cut(s) 691, 791
BtgI CCRYGG 1 cut(s) 693
BtsCI GGATG 2 cut(s) 871, 883
BtsIMutI CAGTG 1 cut(s) 387
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 537
CaiI CAGNNNCTG 1 cut(s) 550
CfoI GCGC 1 cut(s) 871
Cfr13I GGNCC 3 cut(s) 77, 689, 690
CseI GACGC 1 cut(s) 188
Csp6I GTAC 2 cut(s) 854, 902
CviAII CATG 2 cut(s) 127, 694
CviQI GTAC 2 cut(s) 854, 902
DdeI CTNAG 4 cut(s) 20, 269, 349, 810
DpnI GATC 4 cut(s) 97, 713, 744, 958
DpnII GATC 4 cut(s) 95, 711, 742, 956
EaeI YGGCCR 1 cut(s) 789
Eco130I CCWWGG 1 cut(s) 693
Eco24I GRGCYC 1 cut(s) 693
Eco31I GGTCTC 1 cut(s) 831
Eco47I GGWCC 1 cut(s) 77
Eco57I CTGAAG 4 cut(s) 18, 216, 527, 962
EcoRII CCWGG 1 cut(s) 161
EcoT14I CCWWGG 1 cut(s) 693
EcoT38I GRGCYC 1 cut(s) 693
ErhI CCWWGG 1 cut(s) 693
FaeI CATG 2 cut(s) 130, 697
FaqI GGGAC 1 cut(s) 21
FatI CATG 2 cut(s) 126, 693
FbaI TGATCA 1 cut(s) 711
Fnu4HI GCNGC 1 cut(s) 762
FokI GGATG 2 cut(s) 870, 878
FriOI GRGCYC 1 cut(s) 693
Fsp4HI GCNGC 1 cut(s) 762
FspBI CTAG 2 cut(s) 99, 1015
GlaI GCGC 1 cut(s) 870
GluI GCNGC 1 cut(s) 762
GsaI CCCAGC 1 cut(s) 8
HaeIII GGCC 2 cut(s) 691, 791
HgaI GACGC 1 cut(s) 188
HhaI GCGC 1 cut(s) 871
Hin1II CATG 2 cut(s) 130, 697
Hin6I GCGC 1 cut(s) 869
HinP1I GCGC 1 cut(s) 869
HinfI GANTC 2 cut(s) 296, 340
HphI GGTGA 5 cut(s) 125, 291, 349, 464, 1004
Hpy166II GTNNAC 2 cut(s) 854, 904
Hpy188I TCNGA 6 cut(s) 37, 93, 235, 507, 514, 623
Hpy188III TCNNGA 5 cut(s) 75, 99, 446, 674, 923
Hpy8I GTNNAC 2 cut(s) 854, 904
HpyAV CCTTC 2 cut(s) 449, 487
HpyCH4III ACNGT 3 cut(s) 178, 391, 664
HpyCH4IV ACGT 1 cut(s) 913
HpyCH4V TGCA 5 cut(s) 145, 305, 539, 569, 751
HpyF3I CTNAG 4 cut(s) 20, 269, 349, 810
HpySE526I ACGT 1 cut(s) 913
Hsp92II CATG 2 cut(s) 130, 697
HspAI GCGC 1 cut(s) 869
Ksp22I TGATCA 1 cut(s) 711
Kzo9I GATC 4 cut(s) 95, 711, 742, 956
LmnI GCTCC 2 cut(s) 502, 532
Lsp1109I GCAGC 1 cut(s) 773
LweI GCATC 1 cut(s) 856
MaeI CTAG 2 cut(s) 99, 1015
MaeII ACGT 1 cut(s) 913
MaeIII GTNAC 1 cut(s) 261
MalI GATC 4 cut(s) 97, 713, 744, 958
MboI GATC 4 cut(s) 95, 711, 742, 956
MboII GAAGA 2 cut(s) 365, 827
MflI RGATCY 2 cut(s) 95, 956
MhlI GDGCHC 2 cut(s) 587, 693
MlsI TGGCCA 1 cut(s) 791
MluNI TGGCCA 1 cut(s) 791
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 4 cut(s) 328, 442, 558, 892
Mox20I TGGCCA 1 cut(s) 791
MroXI GAANNNNTTC 1 cut(s) 519
MscI TGGCCA 1 cut(s) 791
Msp20I TGGCCA 1 cut(s) 791
MspR9I CCNGG 1 cut(s) 163
Mva1269I GAATGC 1 cut(s) 751
MvaI CCWGG 1 cut(s) 163
NcoI CCATGG 1 cut(s) 693
NdeII GATC 4 cut(s) 95, 711, 742, 956
NlaIII CATG 2 cut(s) 130, 697
NlaIV GGNNCC 3 cut(s) 460, 672, 691
NmuCI GTSAC 1 cut(s) 261
PaqCI CACCTGC 1 cut(s) 310
PctI GAATGC 1 cut(s) 751
PdmI GAANNNNTTC 1 cut(s) 519
PfeI GAWTC 1 cut(s) 340
PkrI GCNGC 1 cut(s) 763
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
Psp6I CCWGG 1 cut(s) 161
PspFI CCCAGC 1 cut(s) 4
PspGI CCWGG 1 cut(s) 161
PspN4I GGNNCC 3 cut(s) 460, 672, 691
PspOMI GGGCCC 1 cut(s) 689
PspPI GGNCC 3 cut(s) 77, 689, 690
PstNI CAGNNNCTG 1 cut(s) 550
PsuI RGATCY 2 cut(s) 95, 956
RsaI GTAC 2 cut(s) 855, 903
RsaNI GTAC 2 cut(s) 854, 902
SatI GCNGC 1 cut(s) 762
Sau3AI GATC 4 cut(s) 95, 711, 742, 956
Sau96I GGNCC 3 cut(s) 77, 689, 690
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 1 cut(s) 163
SduI GDGCHC 2 cut(s) 587, 693
SfaNI GCATC 1 cut(s) 856
SfcI CTRYAG 1 cut(s) 486
SinI GGWCC 1 cut(s) 77
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
SsiI CCGC 2 cut(s) 363, 668
SspI AATATT 2 cut(s) 799, 930
SspMI CTAG 2 cut(s) 99, 1015
StyD4I CCNGG 1 cut(s) 161
StyI CCWWGG 1 cut(s) 693
TaaI ACNGT 3 cut(s) 178, 391, 664
TaiI ACGT 1 cut(s) 916
TaqI TCGA 2 cut(s) 447, 933
TatI WGTACW 1 cut(s) 853
TfiI GAWTC 1 cut(s) 340
TscAI CASTG 1 cut(s) 394
TseFI GTSAC 1 cut(s) 261
TseI GCWGC 1 cut(s) 761
Tsp45I GTSAC 1 cut(s) 261
TspDTI ATGAA 3 cut(s) 128, 209, 469
TspRI CASTG 1 cut(s) 394
VpaK11BI GGWCC 1 cut(s) 77
XapI RAATTY 2 cut(s) 283, 677
XbaI TCTAGA 1 cut(s) 98
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 2 cut(s) 99, 1015
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.