pycom07g02890

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001760
Physical Location & Seq
Reverse (-)
2401445 .. 2403243
1799 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g02890.2

Sequence Viewer

Length: 1554 bp
ATGTATAGTACAATAACGTGTAGGCTCCAGCAGCTCAACTCTATCTCTCAACAGACAGAAACAGGCAGCATTGAGAAACTGAATTCATTGAGGAATAGGGTGAATACAATCATTTCTAATCTAAGTTTTCCCAAAAGCAATATTCCACCTTCTTACAAGGAGAGTCTGGGTGTATTTGAGAAGTACATTATTTCTGTTCTTAGTGTACGTGGAAGGGGAAATTCTGGTCCCTCACAGCAGCATGGACAACGATCTTCTGATGTGCACTCTATGCGTCAATTCGTACAACTTTGTTCTCAAACTTCTCCAGTTCAGACACAGCAAGACGAAAAGAAGCCAACATTTCAGCCAGTGAACTCACAGGATTCTGCAACAACAATGCAGCGGAGCAATGCAACGAACTTGGAAAACGATTCTATGTCTGTATATGAGCACCCAACCCTGCAGTCGACTGAGAAGTATTCAGGAAAGATAGGCTCATTTAACATAGTGCAGCAGGTTTCCTCAGGAAACTTAAATATTCCTAGAAGCAATCTCCAACACGTTAAGACCTTGTTGTCACATAGCCGCGTGCATGGACCACAGTCAAACGTGACTGCTTTTGAGTCAAGCTGTAATACACTTCAAGAGATGTGCCTGAAACAACCTAAAGAAGAGCGGGATGTGCAGACTCAGACACAGAAGCAAGAACTTCAAGATAGGCATGAGATGCTGCACCAATCAGTACAACAAAAACAAATTGTGCAACAGCATAGCAGGCTGGTAAGGTTGCAATCTGGAGCTTCATTTCCTACTGCTTTGCCCAAAACTTATGGTTCTTCCTGCTCACAAGTTCCTCAGTGTTCTACTATTGACAAGAAAAATCTTCCGATGCCTCTAACAGAAGCTAGAACCACTTTCCATCCCATAAACTCACGCTCTATTAAAACTTCTTGTTTGGTTCCCTTGCCTTCATCATCTGTCACGGGAGATTTTGAGAGACCTATTTCTGATACTTCATCCCTATCAAATGCTGGAAGTATTGGAGATGAACAAGTAAATATTGCAGCAAAATCCCCATCACCCATTGCCATCGGTACTCCTGGGATATCTGTTTCACCCTTACTTGAAGAGTCCAGTAACGTCATTCATTGTGATACATCTACAACAATGTCTGATGAGTTGAGTGCTTCTGAGCAGCCTATTCAGCGCTTAATTAAAGTGGTGAACTTGATGTCATCTAAGGCACTAAGTGCTTCTGTTTTGGACATTGGCTCTGTTGTTTGTACAACTGATAGGATATCAGGATCAGCAACACTTACAGGATCTGGAGGTTCAATAGGTGAGGATTTAGCGGGCATGACCAATTCTCGTTTGCAGAAGAGTTATTTAACATGGCAAGGTATGACCTTTGGAACAAAAAATACGAAACGCTGCAGAAGTACAGTGCCAATTGACGTCATTGCGTCAGCAACTGGAAGTATCGGTGATAGTTCAAAGTTAAGTGATATGGAGATATTTGACTTGGAGTCAACTGCAGTATCTTATATCAAAAGGCCAAGGATTGAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

518

Amino Acids

56.34

Weight (kDa)

8.59

Isoelectric Point (pI)

59.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 556
AatII GACGTC 1 cut(s) 1440
Acc36I ACCTGC 1 cut(s) 487
AccBSI CCGCTC 1 cut(s) 658
AccI GTMKAC 1 cut(s) 449
AccII CGCG 1 cut(s) 570
AciI CCGC 4 cut(s) 385, 568, 658, 1334
AclWI GGATC 2 cut(s) 1294, 1312
AcsI RAATTY 2 cut(s) 82, 220
AcyI GRCGYC 1 cut(s) 1437
AdeI CACNNNGTG 1 cut(s) 1232
AfaI GTAC 8 cut(s) 10, 185, 207, 285, 726, 1078, 1267, 1423
AfeI AGCGCT 1 cut(s) 1190
AflIII ACRYGT 2 cut(s) 17, 541
AgsI TTSAA 5 cut(s) 626, 695, 1109, 1317, 1476
AhdI GACNNNNNGTC 1 cut(s) 1507
AjnI CCWGG 1 cut(s) 1081
AluBI AGCT 4 cut(s) 34, 612, 782, 887
AluI AGCT 4 cut(s) 34, 612, 782, 887
Alw21I GWGCWC 2 cut(s) 267, 435
Alw26I GTCTC 1 cut(s) 973
Alw44I GTGCAC 1 cut(s) 263
AlwI GGATC 2 cut(s) 1294, 1312
AlwNI CAGNNNCTG 2 cut(s) 1307, 1454
Aor51HI AGCGCT 1 cut(s) 1190
AoxI GGCC 1 cut(s) 1535
ApaLI GTGCAC 1 cut(s) 263
ApeKI GCWGC 9 cut(s) 31, 66, 238, 382, 493, 712, 1046, 1177, 1413
ApoI RAATTY 2 cut(s) 82, 220
AspLEI GCGC 1 cut(s) 1191
AspS9I GGNCC 2 cut(s) 227, 578
AsuHPI GGTGA 6 cut(s) 112, 1053, 1089, 1216, 1334, 1478
AvaII GGWCC 2 cut(s) 227, 578
AxyI CCTNAGG 1 cut(s) 505
BaeGI GKGCMC 1 cut(s) 267
BaeI ACNNNNGTAYC 2 cut(s) 1503, 1536
Bbv12I GWGCWC 2 cut(s) 267, 435
BbvI GCAGC 9 cut(s) 43, 78, 250, 394, 505, 699, 1058, 1189, 1400
BccI CCATC 3 cut(s) 909, 1066, 1079
BciT130I CCWGG 1 cut(s) 1083
BcoDI GTCTC 1 cut(s) 973
BfaI CTAG 2 cut(s) 525, 888
BfmI CTRYAG 3 cut(s) 443, 1414, 1515
BfoI RGCGCY 1 cut(s) 1192
BfuAI ACCTGC 1 cut(s) 487
Bme1390I CCNGG 1 cut(s) 1083
Bme18I GGWCC 2 cut(s) 227, 578
BmeRI GACNNNNNGTC 1 cut(s) 1507
BmgT120I GGNCC 2 cut(s) 227, 578
BmiI GGNNCC 3 cut(s) 26, 229, 942
BmrFI CCNGG 1 cut(s) 1083
BmsI GCATC 2 cut(s) 699, 861
BoxI GACNNNNGTC 1 cut(s) 583
BpmI CTGGAG 4 cut(s) 11, 291, 798, 1329
BsaAI YACGTR 1 cut(s) 209
BsaHI GRCGYC 1 cut(s) 1437
BsaI GGTCTC 1 cut(s) 973
BsaJI CCNNGG 2 cut(s) 1082, 1538
Bse1I ACTGG 4 cut(s) 308, 350, 1116, 1459
Bse21I CCTNAGG 1 cut(s) 505
Bse3DI GCAATG 3 cut(s) 397, 1065, 1440
BseBI CCWGG 1 cut(s) 1083
BseDI CCNNGG 2 cut(s) 1082, 1538
BseGI GGATG 3 cut(s) 667, 901, 998
BseMI GCAATG 3 cut(s) 397, 1065, 1440
BseMII CTCAG 5 cut(s) 444, 519, 686, 851, 1164
BseNI ACTGG 4 cut(s) 308, 350, 1116, 1459
BseSI GKGCMC 1 cut(s) 267
BseXI GCAGC 9 cut(s) 43, 78, 250, 394, 505, 699, 1058, 1189, 1400
BsgI GTGCAG 3 cut(s) 512, 686, 698
Bsh1236I CGCG 1 cut(s) 570
BshFI GGCC 1 cut(s) 1537
BsiHKAI GWGCWC 2 cut(s) 267, 435
BslFI GGGAC 1 cut(s) 213
BsmAI GTCTC 1 cut(s) 973
BsmFI GGGAC 1 cut(s) 213
BsnI GGCC 1 cut(s) 1537
Bso31I GGTCTC 1 cut(s) 973
Bsp1286I GDGCHC 2 cut(s) 267, 435
Bsp1407I TGTACA 1 cut(s) 1265
Bsp143I GATC 3 cut(s) 251, 1286, 1304
BspACI CCGC 4 cut(s) 385, 568, 658, 1334
BspANI GGCC 1 cut(s) 1537
BspCNI CTCAG 5 cut(s) 445, 518, 685, 850, 1165
BspFNI CGCG 1 cut(s) 570
BspLI GGNNCC 3 cut(s) 26, 229, 942
BspMAI CTGCAG 3 cut(s) 447, 1418, 1519
BspMI ACCTGC 1 cut(s) 487
BspPI GGATC 2 cut(s) 1294, 1312
BspQI GCTCTTC 1 cut(s) 648
BspTNI GGTCTC 1 cut(s) 973
BsrBI CCGCTC 1 cut(s) 658
BsrDI GCAATG 3 cut(s) 397, 1065, 1440
BsrGI TGTACA 1 cut(s) 1265
BsrI ACTGG 4 cut(s) 308, 350, 1116, 1459
BssECI CCNNGG 2 cut(s) 1082, 1538
BssMI GATC 3 cut(s) 251, 1286, 1304
BssNI GRCGYC 1 cut(s) 1437
BssT1I CCWWGG 1 cut(s) 1538
Bst2UI CCWGG 1 cut(s) 1083
Bst4CI ACNGT 2 cut(s) 585, 1426
Bst6I CTCTTC 3 cut(s) 648, 1104, 1355
BstACI GRCGYC 1 cut(s) 1437
BstAPI GCANNNNNTGC 3 cut(s) 271, 709, 1232
BstAUI TGTACA 1 cut(s) 1265
BstBAI YACGTR 1 cut(s) 209
BstC8I GCNNGC 3 cut(s) 572, 758, 1336
BstDEI CTNAG 9 cut(s) 122, 200, 453, 505, 672, 837, 1173, 1221, 1229
BstF5I GGATG 3 cut(s) 667, 901, 998
BstFNI CGCG 1 cut(s) 570
BstH2I RGCGCY 1 cut(s) 1192
BstHHI GCGC 1 cut(s) 1191
BstKTI GATC 3 cut(s) 254, 1289, 1307
BstMAI GTCTC 1 cut(s) 973
BstMBI GATC 3 cut(s) 251, 1286, 1304
BstMWI GCNNNNNNNGC 7 cut(s) 31, 271, 664, 709, 757, 1186, 1232
BstNI CCWGG 1 cut(s) 1083
BstPAI GACNNNNGTC 1 cut(s) 583
BstSCI CCNGG 1 cut(s) 1081
BstSFI CTRYAG 3 cut(s) 443, 1414, 1515
BstSLI GKGCMC 1 cut(s) 267
BstUI CGCG 1 cut(s) 570
BstV1I GCAGC 9 cut(s) 43, 78, 250, 394, 505, 699, 1058, 1189, 1400
BstX2I RGATCY 1 cut(s) 1304
BstYI RGATCY 1 cut(s) 1304
Bsu36I CCTNAGG 1 cut(s) 505
BsuRI GGCC 1 cut(s) 1537
BtsCI GGATG 3 cut(s) 667, 901, 998
BtsIMutI CAGTG 3 cut(s) 357, 845, 1431
BveI ACCTGC 1 cut(s) 487
Cac8I GCNNGC 3 cut(s) 572, 758, 1336
CaiI CAGNNNCTG 2 cut(s) 1307, 1454
CfoI GCGC 1 cut(s) 1191
Cfr13I GGNCC 2 cut(s) 227, 578
CseI GACGC 2 cut(s) 263, 1434
Csp6I GTAC 8 cut(s) 9, 184, 206, 284, 725, 1077, 1266, 1422
CviAII CATG 5 cut(s) 242, 575, 704, 1339, 1374
CviQI GTAC 8 cut(s) 9, 184, 206, 284, 725, 1077, 1266, 1422
DdeI CTNAG 9 cut(s) 122, 200, 453, 505, 672, 837, 1173, 1221, 1229
DpnI GATC 3 cut(s) 253, 1288, 1306
DpnII GATC 3 cut(s) 251, 1286, 1304
DraIII CACNNNGTG 1 cut(s) 1232
DrdI GACNNNNNNGTC 1 cut(s) 556
DriI GACNNNNNGTC 1 cut(s) 1507
DseDI GACNNNNNNGTC 1 cut(s) 556
Eam1104I CTCTTC 3 cut(s) 648, 1104, 1355
Eam1105I GACNNNNNGTC 1 cut(s) 1507
EarI CTCTTC 3 cut(s) 648, 1104, 1355
Eco130I CCWWGG 1 cut(s) 1538
Eco31I GGTCTC 1 cut(s) 973
Eco32I GATATC 2 cut(s) 1089, 1281
Eco47I GGWCC 2 cut(s) 227, 578
Eco47III AGCGCT 1 cut(s) 1190
Eco81I CCTNAGG 1 cut(s) 505
EcoRI GAATTC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 1081
EcoRV GATATC 2 cut(s) 1089, 1281
EcoT14I CCWWGG 1 cut(s) 1538
ErhI CCWWGG 1 cut(s) 1538
FaeI CATG 5 cut(s) 245, 578, 707, 1342, 1377
FaqI GGGAC 1 cut(s) 213
FatI CATG 5 cut(s) 241, 574, 703, 1338, 1373
FauI CCCGC 2 cut(s) 651, 1327
FblI GTMKAC 1 cut(s) 449
FokI GGATG 3 cut(s) 674, 888, 985
FspBI CTAG 2 cut(s) 525, 888
GlaI GCGC 1 cut(s) 1190
GsuI CTGGAG 4 cut(s) 11, 291, 798, 1329
HaeII RGCGCY 1 cut(s) 1192
HaeIII GGCC 1 cut(s) 1537
HgaI GACGC 2 cut(s) 263, 1434
HhaI GCGC 1 cut(s) 1191
Hin1I GRCGYC 1 cut(s) 1437
Hin1II CATG 5 cut(s) 245, 578, 707, 1342, 1377
Hin6I GCGC 1 cut(s) 1189
HinP1I GCGC 1 cut(s) 1189
HincII GTYRAC 2 cut(s) 450, 1512
HindII GTYRAC 2 cut(s) 450, 1512
HinfI GANTC 7 cut(s) 163, 365, 413, 605, 670, 1112, 1508
HphI GGTGA 6 cut(s) 112, 1053, 1089, 1216, 1334, 1478
Hpy166II GTNNAC 6 cut(s) 206, 265, 355, 450, 1207, 1512
Hpy188I TCNGA 7 cut(s) 259, 315, 675, 870, 991, 1156, 1174
Hpy188III TCNNGA 7 cut(s) 465, 507, 626, 695, 777, 1284, 1308
Hpy8I GTNNAC 6 cut(s) 206, 265, 355, 450, 1207, 1512
HpyAV CCTTC 3 cut(s) 159, 207, 960
HpyCH4III ACNGT 2 cut(s) 585, 1426
HpyCH4IV ACGT 6 cut(s) 17, 208, 543, 591, 1122, 1437
HpyF10VI GCNNNNNNNGC 7 cut(s) 31, 271, 664, 709, 757, 1186, 1232
HpyF3I CTNAG 9 cut(s) 122, 200, 453, 505, 672, 837, 1173, 1221, 1229
HpySE526I ACGT 6 cut(s) 17, 208, 543, 591, 1122, 1437
Hsp92I GRCGYC 1 cut(s) 1437
Hsp92II CATG 5 cut(s) 245, 578, 707, 1342, 1377
HspAI GCGC 1 cut(s) 1189
Kzo9I GATC 3 cut(s) 251, 1286, 1304
LguI GCTCTTC 1 cut(s) 648
LmnI GCTCC 3 cut(s) 30, 387, 779
Lsp1109I GCAGC 9 cut(s) 43, 78, 250, 394, 505, 699, 1058, 1189, 1400
LweI GCATC 2 cut(s) 699, 861
MaeI CTAG 2 cut(s) 525, 888
MaeII ACGT 6 cut(s) 17, 208, 543, 591, 1122, 1437
MaeIII GTNAC 4 cut(s) 558, 592, 961, 1118
MalI GATC 3 cut(s) 253, 1288, 1306
MbiI CCGCTC 1 cut(s) 658
MboI GATC 3 cut(s) 251, 1286, 1304
MboII GAAGA 6 cut(s) 246, 665, 810, 857, 1121, 1372
MfeI CAATTG 1 cut(s) 1431
MflI RGATCY 1 cut(s) 1304
MhlI GDGCHC 2 cut(s) 267, 435
MluCI AATT 7 cut(s) 82, 220, 278, 738, 1194, 1345, 1431
MlyI GAGTC 5 cut(s) 172, 614, 664, 1121, 1517
MmeI TCCRAC 1 cut(s) 562
MnlI CCTC 8 cut(s) 84, 241, 514, 846, 885, 1304, 1318, 1540
MseI TTAA 8 cut(s) 483, 515, 546, 924, 1193, 1197, 1370, 1481
MspA1I CMGCKG 1 cut(s) 385
MspR9I CCNGG 1 cut(s) 1083
MunI CAATTG 1 cut(s) 1431
MvaI CCWGG 1 cut(s) 1083
MvnI CGCG 1 cut(s) 570
MwoI GCNNNNNNNGC 7 cut(s) 31, 271, 664, 709, 757, 1186, 1232
NdeII GATC 3 cut(s) 251, 1286, 1304
NlaIII CATG 5 cut(s) 245, 578, 707, 1342, 1377
NlaIV GGNNCC 3 cut(s) 26, 229, 942
NmuCI GTSAC 3 cut(s) 558, 592, 961
PacI TTAATTAA 1 cut(s) 1197
PciSI GCTCTTC 1 cut(s) 648
PfeI GAWTC 2 cut(s) 365, 413
PleI GAGTC 5 cut(s) 171, 613, 664, 1120, 1516
PpsI GAGTC 5 cut(s) 171, 613, 664, 1120, 1516
Ppu21I YACGTR 1 cut(s) 209
PshAI GACNNNNGTC 1 cut(s) 583
Psp6I CCWGG 1 cut(s) 1081
PspGI CCWGG 1 cut(s) 1081
PspN4I GGNNCC 3 cut(s) 26, 229, 942
PspPI GGNCC 2 cut(s) 227, 578
PsrI GAACNNNNNNTAC 2 cut(s) 1387, 1419
PstI CTGCAG 3 cut(s) 447, 1418, 1519
PstNI CAGNNNCTG 2 cut(s) 1307, 1454
PsuI RGATCY 1 cut(s) 1304
RsaI GTAC 8 cut(s) 10, 185, 207, 285, 726, 1078, 1267, 1423
RsaNI GTAC 8 cut(s) 9, 184, 206, 284, 725, 1077, 1266, 1422
SalI GTCGAC 1 cut(s) 448
SapI GCTCTTC 1 cut(s) 648
SaqAI TTAA 8 cut(s) 483, 515, 546, 924, 1193, 1197, 1370, 1481
Sau3AI GATC 3 cut(s) 251, 1286, 1304
Sau96I GGNCC 2 cut(s) 227, 578
SchI GAGTC 5 cut(s) 172, 614, 664, 1121, 1517
ScrFI CCNGG 1 cut(s) 1083
SduI GDGCHC 2 cut(s) 267, 435
SfaNI GCATC 2 cut(s) 699, 861
SfcI CTRYAG 3 cut(s) 443, 1414, 1515
SinI GGWCC 2 cut(s) 227, 578
Sse9I AATT 7 cut(s) 82, 220, 278, 738, 1194, 1345, 1431
SsiI CCGC 4 cut(s) 385, 568, 658, 1334
SspI AATATT 3 cut(s) 142, 520, 1042
SspMI CTAG 2 cut(s) 525, 888
StyD4I CCNGG 1 cut(s) 1081
StyI CCWWGG 1 cut(s) 1538
TaaI ACNGT 2 cut(s) 585, 1426
TaiI ACGT 6 cut(s) 20, 211, 546, 594, 1125, 1440
TaqI TCGA 1 cut(s) 449
TasI AATT 7 cut(s) 82, 220, 278, 738, 1194, 1345, 1431
TatI WGTACW 5 cut(s) 8, 183, 724, 1265, 1421
TauI GCSGC 1 cut(s) 570
TfiI GAWTC 2 cut(s) 365, 413
Tru1I TTAA 8 cut(s) 483, 515, 546, 924, 1193, 1197, 1370, 1481
Tru9I TTAA 8 cut(s) 483, 515, 546, 924, 1193, 1197, 1370, 1481
TscAI CASTG 3 cut(s) 357, 845, 1431
TseFI GTSAC 3 cut(s) 558, 592, 961
TseI GCWGC 9 cut(s) 31, 66, 238, 382, 493, 712, 1046, 1177, 1413
Tsp45I GTSAC 3 cut(s) 558, 592, 961
TspDTI ATGAA 6 cut(s) 75, 774, 942, 987, 1044, 1118
TspRI CASTG 3 cut(s) 357, 845, 1431
VneI GTGCAC 1 cut(s) 263
VpaK11BI GGWCC 2 cut(s) 227, 578
XapI RAATTY 2 cut(s) 82, 220
XmiI GTMKAC 1 cut(s) 449
XspI CTAG 2 cut(s) 525, 888
ZraI GACGTC 1 cut(s) 1438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.