pycom07g02900

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001760
Physical Location & Seq
Reverse (-)
2404217 .. 2404987
771 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g02900.1

Sequence Viewer

Length: 771 bp
ATGTTGCAAACTCCTGTCATGTGCCCTTCGGAGAAGCGCGTTGCTCCACTTTCTGATATTTGGCAGGATCAGATGTCTTATTTCGACAAGACAACACAGCCGGGGAATAAGACTCAGTCACAGATAGCCTTTAGGCAAACAAACCATTCACAAGTGGCTCTGGATAATCATCAAAATGTGTCATCAAGTTCACAGAACCCATTAATGCAATCACAAAAGCAAGCACATTTATTAGGGAGAAAGTTGACTGCTACTAATATGCAACAGAGCCACTTAGATGGGCAACCAAATGGCTTCCCTAACTTGCACCAGCAACAGCCCAGGTTACCATGCCAACAGAATACTGTACAACAGCAGTTTCAGGTACAGAAAATAAATCATTCAAGTGCACATCATGAGCAATTGAGTGAAAGAAACAATATGTATGTACTACCACAAAGCTCACAGCTGCTTGAAACACAGTGTGCCAGCTCAGGCATGCGCCTATTCGAGGTTAAAATGGAGGGGCAAACGCAACAGAAAGCTTCAGTTCTGCTGCCAAATCAAGATCAAGAATCACAAGCAGATTCTCTTGAGAGACGACTGAACTCACAAACACAGCCACAGAAGATGGAACAACTGCCAAATCCATCAGCAGAAGATCAGCAGCAAACATATCAAACATCAGGTAAAACTGTGAAACCGAATTATGTATCCGCAGAAAAAGTTGATTTATTAAATAAGAAAATAAGTAGAAATACCGCCCTAGATTCATTGTTGATAAATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

257

Amino Acids

29.08

Weight (kDa)

8.4

Isoelectric Point (pI)

57.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 39
AciI CCGC 2 cut(s) 696, 741
AclWI GGATC 1 cut(s) 75
AcuI CTGAAG 1 cut(s) 510
AdeI CACNNNGTG 1 cut(s) 464
AfaI GTAC 3 cut(s) 348, 366, 429
AfiI CCNNNNNNNGG 1 cut(s) 490
AgsI TTSAA 2 cut(s) 384, 455
AjnI CCWGG 1 cut(s) 320
AluBI AGCT 4 cut(s) 441, 448, 471, 524
AluI AGCT 4 cut(s) 441, 448, 471, 524
Alw21I GWGCWC 1 cut(s) 391
Alw26I GTCTC 1 cut(s) 571
Alw44I GTGCAC 1 cut(s) 387
AlwI GGATC 1 cut(s) 75
ApaLI GTGCAC 1 cut(s) 387
ApeKI GCWGC 3 cut(s) 448, 535, 646
AseI ATTAAT 1 cut(s) 203
AspLEI GCGC 2 cut(s) 39, 483
AsuC2I CCSGG 1 cut(s) 102
BaeGI GKGCMC 2 cut(s) 26, 391
Bbv12I GWGCWC 1 cut(s) 391
BbvI GCAGC 3 cut(s) 435, 522, 658
BccI CCATC 3 cut(s) 272, 604, 637
BciT130I CCWGG 1 cut(s) 322
BciVI GTATCC 1 cut(s) 703
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 571
BfaI CTAG 1 cut(s) 746
BfuI GTATCC 1 cut(s) 703
BisI GCNGC 3 cut(s) 449, 536, 647
BlsI GCNGC 3 cut(s) 450, 537, 648
Bme1390I CCNGG 2 cut(s) 102, 322
BmrFI CCNGG 2 cut(s) 102, 322
Bpu10I CCTNAGC 1 cut(s) 472
BpuEI CTTGAG 1 cut(s) 593
BpuMI CCSGG 1 cut(s) 102
BsaBI GATNNNNATC 1 cut(s) 168
BsaJI CCNNGG 2 cut(s) 101, 320
Bsc4I CCNNNNNNNGG 1 cut(s) 490
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 2 cut(s) 101, 320
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 1 cut(s) 490
BseMII CTCAG 2 cut(s) 128, 486
BseSI GKGCMC 2 cut(s) 26, 391
BseXI GCAGC 3 cut(s) 435, 522, 658
Bsh1236I CGCG 1 cut(s) 39
BsiHKAI GWGCWC 1 cut(s) 391
BsiSI CCGG 1 cut(s) 101
BslI CCNNNNNNNGG 1 cut(s) 490
BsmAI GTCTC 1 cut(s) 571
BsmBI CGTCTC 1 cut(s) 571
Bsp1286I GDGCHC 2 cut(s) 26, 391
Bsp1407I TGTACA 1 cut(s) 346
Bsp143I GATC 3 cut(s) 67, 547, 640
BspACI CCGC 2 cut(s) 696, 741
BspCNI CTCAG 2 cut(s) 127, 485
BspFNI CGCG 1 cut(s) 39
BspHI TCATGA 1 cut(s) 394
BspPI GGATC 1 cut(s) 75
BsrGI TGTACA 1 cut(s) 346
BssECI CCNNGG 2 cut(s) 101, 320
BssMI GATC 3 cut(s) 67, 547, 640
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 3 cut(s) 346, 462, 676
BstAUI TGTACA 1 cut(s) 346
BstC8I GCNNGC 3 cut(s) 222, 469, 479
BstDEI CTNAG 3 cut(s) 114, 274, 472
BstEII GGTNACC 1 cut(s) 324
BstENI CCTNNNNNAGG 1 cut(s) 488
BstFNI CGCG 1 cut(s) 39
BstHHI GCGC 2 cut(s) 39, 483
BstKTI GATC 3 cut(s) 70, 550, 643
BstMAI GTCTC 1 cut(s) 571
BstMBI GATC 3 cut(s) 67, 547, 640
BstNI CCWGG 1 cut(s) 322
BstNSI RCATGY 1 cut(s) 481
BstPI GGTNACC 1 cut(s) 324
BstSCI CCNGG 2 cut(s) 100, 320
BstSLI GKGCMC 2 cut(s) 26, 391
BstUI CGCG 1 cut(s) 39
BstV1I GCAGC 3 cut(s) 435, 522, 658
BstXI CCANNNNNNTGG 1 cut(s) 278
BsuI GTATCC 1 cut(s) 703
BtsIMutI CAGTG 1 cut(s) 467
Cac8I GCNNGC 3 cut(s) 222, 469, 479
CciI TCATGA 1 cut(s) 394
CfoI GCGC 2 cut(s) 39, 483
Csp6I GTAC 3 cut(s) 347, 365, 428
CviAII CATG 4 cut(s) 19, 330, 395, 478
CviQI GTAC 3 cut(s) 347, 365, 428
DdeI CTNAG 3 cut(s) 114, 274, 472
DpnI GATC 3 cut(s) 69, 549, 642
DpnII GATC 3 cut(s) 67, 547, 640
DraIII CACNNNGTG 1 cut(s) 464
Eco57I CTGAAG 1 cut(s) 510
Eco91I GGTNACC 1 cut(s) 324
EcoNI CCTNNNNNAGG 1 cut(s) 488
EcoO65I GGTNACC 1 cut(s) 324
EcoRII CCWGG 1 cut(s) 320
Esp3I CGTCTC 1 cut(s) 571
FaeI CATG 4 cut(s) 22, 333, 398, 481
FaiI YATR 9 cut(s) 20, 260, 331, 396, 422, 426, 479, 655, 690
FatI CATG 4 cut(s) 18, 329, 394, 477
Fnu4HI GCNGC 3 cut(s) 449, 536, 647
Fsp4HI GCNGC 3 cut(s) 449, 536, 647
FspBI CTAG 1 cut(s) 746
GlaI GCGC 2 cut(s) 38, 482
GluI GCNGC 3 cut(s) 449, 536, 647
HapII CCGG 1 cut(s) 101
HhaI GCGC 2 cut(s) 39, 483
Hin1II CATG 4 cut(s) 22, 333, 398, 481
Hin6I GCGC 2 cut(s) 37, 481
HinP1I GCGC 2 cut(s) 37, 481
HincII GTYRAC 1 cut(s) 246
HindII GTYRAC 1 cut(s) 246
HindIII AAGCTT 1 cut(s) 522
HinfI GANTC 4 cut(s) 112, 554, 566, 749
HpaII CCGG 1 cut(s) 101
Hpy166II GTNNAC 3 cut(s) 191, 246, 389
Hpy188I TCNGA 3 cut(s) 31, 55, 72
Hpy188III TCNNGA 5 cut(s) 161, 395, 545, 551, 572
Hpy8I GTNNAC 3 cut(s) 191, 246, 389
HpyAV CCTTC 1 cut(s) 36
HpyCH4III ACNGT 3 cut(s) 346, 462, 676
HpyCH4V TGCA 5 cut(s) 7, 208, 262, 307, 389
HpyF3I CTNAG 3 cut(s) 114, 274, 472
Hsp92II CATG 4 cut(s) 22, 333, 398, 481
HspAI GCGC 2 cut(s) 37, 481
Kzo9I GATC 3 cut(s) 67, 547, 640
LmnI GCTCC 1 cut(s) 49
Lsp1109I GCAGC 3 cut(s) 435, 522, 658
MaeI CTAG 1 cut(s) 746
MaeIII GTNAC 2 cut(s) 117, 324
MalI GATC 3 cut(s) 69, 549, 642
MboI GATC 3 cut(s) 67, 547, 640
MboII GAAGA 2 cut(s) 619, 650
MfeI CAATTG 1 cut(s) 401
MhlI GDGCHC 2 cut(s) 26, 391
MluCI AATT 3 cut(s) 401, 685, 763
MlyI GAGTC 1 cut(s) 106
MnlI CCTC 2 cut(s) 484, 496
MseI TTAA 3 cut(s) 203, 495, 716
MslI CAYNNNNRTG 3 cut(s) 174, 276, 384
MspA1I CMGCKG 1 cut(s) 448
MspI CCGG 1 cut(s) 101
MspR9I CCNGG 2 cut(s) 102, 322
MunI CAATTG 1 cut(s) 401
MvaI CCWGG 1 cut(s) 322
MvnI CGCG 1 cut(s) 39
NciI CCSGG 1 cut(s) 102
NdeII GATC 3 cut(s) 67, 547, 640
NlaIII CATG 4 cut(s) 22, 333, 398, 481
NmuCI GTSAC 1 cut(s) 117
NspI RCATGY 1 cut(s) 481
PaeI GCATGC 1 cut(s) 481
PagI TCATGA 1 cut(s) 394
PfeI GAWTC 3 cut(s) 554, 566, 749
PflFI GACNNNGTC 1 cut(s) 115
PkrI GCNGC 3 cut(s) 450, 537, 648
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PshBI ATTAAT 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 320
PspEI GGTNACC 1 cut(s) 324
PspGI CCWGG 1 cut(s) 320
PsyI GACNNNGTC 1 cut(s) 115
PvuII CAGCTG 1 cut(s) 448
RsaI GTAC 3 cut(s) 348, 366, 429
RsaNI GTAC 3 cut(s) 347, 365, 428
RseI CAYNNNNRTG 3 cut(s) 174, 276, 384
SaqAI TTAA 3 cut(s) 203, 495, 716
SatI GCNGC 3 cut(s) 449, 536, 647
Sau3AI GATC 3 cut(s) 67, 547, 640
SchI GAGTC 1 cut(s) 106
ScrFI CCNGG 2 cut(s) 102, 322
SduI GDGCHC 2 cut(s) 26, 391
SetI ASST 8 cut(s) 326, 366, 443, 450, 473, 495, 526, 670
SmiMI CAYNNNNRTG 3 cut(s) 174, 276, 384
SmlI CTYRAG 1 cut(s) 572
SmoI CTYRAG 1 cut(s) 572
SphI GCATGC 1 cut(s) 481
Sse9I AATT 3 cut(s) 401, 685, 763
SsiI CCGC 2 cut(s) 696, 741
SspMI CTAG 1 cut(s) 746
StyD4I CCNGG 2 cut(s) 100, 320
TaaI ACNGT 3 cut(s) 346, 462, 676
TaqI TCGA 2 cut(s) 84, 489
TasI AATT 3 cut(s) 401, 685, 763
TatI WGTACW 2 cut(s) 346, 427
TfiI GAWTC 3 cut(s) 554, 566, 749
Tru1I TTAA 3 cut(s) 203, 495, 716
Tru9I TTAA 3 cut(s) 203, 495, 716
TscAI CASTG 1 cut(s) 467
TseFI GTSAC 1 cut(s) 117
TseI GCWGC 3 cut(s) 448, 535, 646
Tsp45I GTSAC 1 cut(s) 117
TspDTI ATGAA 1 cut(s) 741
TspRI CASTG 1 cut(s) 467
Tth111I GACNNNGTC 1 cut(s) 115
VneI GTGCAC 1 cut(s) 387
VspI ATTAAT 1 cut(s) 203
XagI CCTNNNNNAGG 1 cut(s) 488
XceI RCATGY 1 cut(s) 481
XspI CTAG 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.