Rh4BG156200

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001760
Physical Location & Seq
Reverse (-)
28225294 .. 28225969
676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG156200.1

Sequence Viewer

Length: 423 bp
ATGTATGAAAAGAAGAATCTTCCAATGCTTCCTATAAAACCTAAATCTCCTGTCATTTCAAGAAGCTCACTGAAGGCTGGAATTTCTTCTTTGGCTCCCTTAGCTTCAGCACCTATACCAGGGGATTCTGAGAAACCTATGTCTGAAACTTCGTTGGTTTCAAATGCAGTAAATATTGGAGGTCAACAAGCATATGGTGCTCCAGAAGCCATCCATTGTTCTTTTGATGGGTTGAATGCTGCTGAGCAACCTATTCAGAGATTGATTAAAATGTCAACTTCACTTGTTCCCCACTGTTGCTCTGGCAGTGATGATCAAGATCTTTCAGCAAAGGTAAAGTCGAAGCTCAATTTCTCTTTCTGGAACCTGATGGAACCCTTGTCACTTGGGGAGATAGAAAGGACATGGGATGTATGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

140

Amino Acids

15.02

Weight (kDa)

6.09

Isoelectric Point (pI)

53.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 81
AcuI CTGAAG 2 cut(s) 90, 92
AfiI CCNNNNNNNGG 1 cut(s) 119
AgsI TTSAA 3 cut(s) 60, 162, 235
AjnI CCWGG 1 cut(s) 118
AluBI AGCT 3 cut(s) 66, 104, 346
AluI AGCT 3 cut(s) 66, 104, 346
Alw21I GWGCWC 1 cut(s) 202
ApeKI GCWGC 1 cut(s) 239
ApoI RAATTY 1 cut(s) 81
ArsI GACNNNNNNTTYG 2 cut(s) 323, 355
Asp700I GAANNNNTTC 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 202
BbvI GCAGC 1 cut(s) 226
BccI CCATC 3 cut(s) 218, 221, 364
BciT130I CCWGG 1 cut(s) 120
BclI TGATCA 1 cut(s) 313
BglII AGATCT 1 cut(s) 319
BisI GCNGC 1 cut(s) 240
BlpI GCTNAGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 241
Bme1390I CCNGG 1 cut(s) 120
BmiI GGNNCC 3 cut(s) 96, 365, 375
BmrFI CCNGG 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 186
Bpu10I CCTNAGC 1 cut(s) 100
Bpu1102I GCTNAGC 1 cut(s) 243
BsaBI GATNNNNATC 1 cut(s) 318
BsaJI CCNNGG 1 cut(s) 119
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse8I GATNNNNATC 1 cut(s) 318
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 1 cut(s) 119
BseGI GGATG 2 cut(s) 210, 415
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMII CTCAG 2 cut(s) 120, 234
BseXI GCAGC 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 202
BslI CCNNNNNNNGG 1 cut(s) 119
BsmI GAATGC 1 cut(s) 241
Bsp1286I GDGCHC 1 cut(s) 202
Bsp143I GATC 2 cut(s) 313, 319
Bsp1720I GCTNAGC 1 cut(s) 243
BspCNI CTCAG 2 cut(s) 121, 235
BspLI GGNNCC 3 cut(s) 96, 365, 375
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 2 cut(s) 313, 319
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 1 cut(s) 296
BstAPI GCANNNNNTGC 1 cut(s) 197
BstDEI CTNAG 3 cut(s) 100, 129, 243
BstENI CCTNNNNNAGG 1 cut(s) 117
BstF5I GGATG 2 cut(s) 210, 415
BstKTI GATC 2 cut(s) 316, 322
BstMBI GATC 2 cut(s) 313, 319
BstMWI GCNNNNNNNGC 3 cut(s) 101, 197, 206
BstNI CCWGG 1 cut(s) 120
BstSCI CCNGG 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 226
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
BtsCI GGATG 2 cut(s) 210, 415
BtsI GCAGTG 1 cut(s) 313
BtsIMutI CAGTG 3 cut(s) 68, 292, 313
CviAII CATG 1 cut(s) 405
CviJI RGCY 6 cut(s) 66, 77, 95, 104, 209, 346
CviKI_1 RGCY 6 cut(s) 66, 77, 95, 104, 209, 346
DdeI CTNAG 3 cut(s) 100, 129, 243
DpnI GATC 2 cut(s) 315, 321
DpnII GATC 2 cut(s) 313, 319
Eco57I CTGAAG 2 cut(s) 90, 92
EcoNI CCTNNNNNAGG 1 cut(s) 117
EcoRII CCWGG 1 cut(s) 118
FaeI CATG 1 cut(s) 408
FaiI YATR 8 cut(s) 6, 35, 116, 140, 193, 195, 406, 415
FatI CATG 1 cut(s) 404
FauNDI CATATG 1 cut(s) 193
FbaI TGATCA 1 cut(s) 313
Fnu4HI GCNGC 1 cut(s) 240
FokI GGATG 1 cut(s) 197
Fsp4HI GCNGC 1 cut(s) 240
GluI GCNGC 1 cut(s) 240
GsuI CTGGAG 1 cut(s) 186
Hin1II CATG 1 cut(s) 408
HincII GTYRAC 2 cut(s) 185, 276
HindII GTYRAC 2 cut(s) 185, 276
HinfI GANTC 2 cut(s) 16, 125
Hpy166II GTNNAC 2 cut(s) 185, 276
Hpy188I TCNGA 3 cut(s) 130, 145, 258
Hpy188III TCNNGA 4 cut(s) 60, 203, 317, 361
Hpy8I GTNNAC 2 cut(s) 185, 276
HpyAV CCTTC 1 cut(s) 67
HpyCH4III ACNGT 1 cut(s) 296
HpyCH4V TGCA 1 cut(s) 167
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 197, 206
HpyF3I CTNAG 3 cut(s) 100, 129, 243
Hsp92II CATG 1 cut(s) 408
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 2 cut(s) 313, 319
LmnI GCTCC 2 cut(s) 100, 205
LpnPI CCDG 8 cut(s) 63, 63, 105, 132, 216, 288, 346, 380
Lsp1109I GCAGC 1 cut(s) 226
MaeIII GTNAC 1 cut(s) 381
MalI GATC 2 cut(s) 315, 321
MboI GATC 2 cut(s) 313, 319
MboII GAAGA 3 cut(s) 11, 25, 78
MflI RGATCY 1 cut(s) 319
MhlI GDGCHC 1 cut(s) 202
MluCI AATT 2 cut(s) 81, 349
MnlI CCTC 1 cut(s) 173
MroXI GAANNNNTTC 1 cut(s) 85
MseI TTAA 1 cut(s) 267
MspR9I CCNGG 1 cut(s) 120
Mva1269I GAATGC 1 cut(s) 241
MvaI CCWGG 1 cut(s) 120
MwoI GCNNNNNNNGC 3 cut(s) 101, 197, 206
NdeI CATATG 1 cut(s) 193
NdeII GATC 2 cut(s) 313, 319
NlaIII CATG 1 cut(s) 408
NlaIV GGNNCC 3 cut(s) 96, 365, 375
NmuCI GTSAC 1 cut(s) 381
PctI GAATGC 1 cut(s) 241
PdmI GAANNNNTTC 1 cut(s) 85
PfeI GAWTC 2 cut(s) 16, 125
PkrI GCNGC 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 3 cut(s) 96, 365, 375
PsuI RGATCY 1 cut(s) 319
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 1 cut(s) 240
Sau3AI GATC 2 cut(s) 313, 319
ScrFI CCNGG 1 cut(s) 120
SduI GDGCHC 1 cut(s) 202
Sse9I AATT 2 cut(s) 81, 349
SspI AATATT 1 cut(s) 175
StyD4I CCNGG 1 cut(s) 118
TaaI ACNGT 1 cut(s) 296
TaqI TCGA 1 cut(s) 341
TasI AATT 2 cut(s) 81, 349
TfiI GAWTC 2 cut(s) 16, 125
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 3 cut(s) 75, 299, 313
TseFI GTSAC 1 cut(s) 381
TseI GCWGC 1 cut(s) 239
Tsp45I GTSAC 1 cut(s) 381
TspDTI ATGAA 1 cut(s) 21
TspRI CASTG 3 cut(s) 75, 299, 313
XagI CCTNNNNNAGG 1 cut(s) 117
XapI RAATTY 1 cut(s) 81
XcmI CCANNNNNNNNNTGG 1 cut(s) 299
XmnI GAANNNNTTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.