Rorug04G0095100

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001760
Physical Location & Seq
Reverse (-)
15015596 .. 15018397
2802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0095100.1

Sequence Viewer

Length: 993 bp
ATGGTGGATCTCAGCCCCACGTCTGAAATCTTAAGTCCGGCAAGTATGCGGAGCGTGTCGGCGTCAGAGCTCCGGTGTATCTCTCTGCCGTCCTTCAATACCTGGCTGCTGTGGTTTTGTTTTGCATTTCAGGGGTCTTTACTGATTGAGCTTATTCAGCAGATGCAAGTACAAGGTGTGCAGATTCTTGATTGTGATAGGCGACCTACTGTGACGGATGATAGTGATGCCACAAAGATGGGTTCAAGGGGCGATGATGAGAAGCAATATCTTGTAAAGTGGCAAGAACTTCCTTACGATGAATGCTATTGGGAAGTGGAGGCTGATATCTCTACATTTCAGCCAGAAATAGAGAAATTCCATAGAATCCAGTCTAGATCTCACAAATTGTCTGGTAAGCAGAAGAGCAGTCTTAAAGATGCTCTCGAATCAAAAAAGAAGCAGAAAGAGTTTCAACAGTTTGAACACAGTCCTGAATTTCTTTCTGGAGGTACACTGCATCCCTATCAGCTTGAAGGTTTGAACTTTTTACGCTTCTCCTGGTCCAAACGAACTCCTGTGATACTTGCAGACGAGATGGGACTTGGAAAAACTATACAGAGTATAGCTTTTTTAGCATCTCTGTATGAAGAGAAAGTCTATCCACATCTAGTGGTGAGGAGAGGAGCAGACAAAGCGGAGATTTACAGTCTTGCTTTCTCTTCCACTGCCCAATGGCTAGCTGTCTCAAGTGACAAGGGTACTGTCCGTGTTTTCAGCGTAAAGGTTGACTCAGGATCTTTGGGGAATGATGGATCATGCAGTACATCAGAAATTAGTGGTTCTAATTTATCAGGCATTTCATCTCTGTCTTACATCAAAGCTGTCCTTCCAAGGTATTTTAGTTCAAAATGGTCAGTGGCCAAATTTCGCTTGCAGGAAGGTGTACGGTACATTGTTGCTTTTGGCCACCAGAAGAACACAGTTATCATTGTTGGCATGGATGGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

330

Amino Acids

37.12

Weight (kDa)

7.01

Isoelectric Point (pI)

47.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chromo PF00385 85 - 122 6.9e-12 Chromo (CHRromatin Organisation MOdifier) domain
SNF2-rel_dom PF00176 169 - 213 1.8e-10 SNF2-related domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 49, 677
AclWI GGATC 3 cut(s) 15, 784, 802
AcoI YGGCCR 2 cut(s) 900, 946
AcsI RAATTY 3 cut(s) 356, 476, 905
AcyI GRCGYC 1 cut(s) 62
AfaI GTAC 6 cut(s) 171, 493, 742, 805, 927, 932
AflII CTTAAG 1 cut(s) 31
AgsI TTSAA 7 cut(s) 97, 246, 455, 464, 515, 523, 888
AjiI CACGTC 1 cut(s) 21
AjnI CCWGG 2 cut(s) 101, 539
AluBI AGCT 6 cut(s) 70, 151, 511, 608, 722, 863
AluI AGCT 6 cut(s) 70, 151, 511, 608, 722, 863
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 1 cut(s) 730
AlwI GGATC 3 cut(s) 15, 784, 802
AoxI GGCC 2 cut(s) 900, 946
ApeKI GCWGC 1 cut(s) 106
ApoI RAATTY 3 cut(s) 356, 476, 905
AspS9I GGNCC 1 cut(s) 543
AsuHPI GGTGA 1 cut(s) 667
AsuNHI GCTAGC 1 cut(s) 718
AvaII GGWCC 1 cut(s) 543
BalI TGGCCA 2 cut(s) 902, 948
BanII GRGCYC 1 cut(s) 72
Bbv12I GWGCWC 1 cut(s) 72
BbvI GCAGC 1 cut(s) 93
BccI CCATC 4 cut(s) 232, 571, 785, 977
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 2 cut(s) 103, 541
BcoDI GTCTC 1 cut(s) 730
BfaI CTAG 3 cut(s) 375, 650, 719
BfrI CTTAAG 1 cut(s) 31
BglII AGATCT 1 cut(s) 377
BisI GCNGC 1 cut(s) 107
BlsI GCNGC 1 cut(s) 108
Bme1390I CCNGG 2 cut(s) 103, 541
Bme18I GGWCC 1 cut(s) 543
BmgBI CACGTC 1 cut(s) 21
BmgT120I GGNCC 1 cut(s) 543
BmrFI CCNGG 2 cut(s) 103, 541
BmsI GCATC 5 cut(s) 153, 217, 409, 508, 626
BmtI GCTAGC 1 cut(s) 722
BpmI CTGGAG 1 cut(s) 507
BpuEI CTTGAG 1 cut(s) 712
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 872
BsaWI WCCGGW 1 cut(s) 72
Bse1I ACTGG 1 cut(s) 370
BseBI CCWGG 2 cut(s) 103, 541
BseDI CCNNGG 1 cut(s) 872
BseGI GGATG 3 cut(s) 223, 499, 988
BseMII CTCAG 2 cut(s) 25, 786
BseNI ACTGG 1 cut(s) 370
BseRI GAGGAG 2 cut(s) 673, 678
BseXI GCAGC 1 cut(s) 93
BsgI GTGCAG 1 cut(s) 200
BshFI GGCC 2 cut(s) 902, 948
BsiHKAI GWGCWC 1 cut(s) 72
BsiSI CCGG 2 cut(s) 38, 73
BslFI GGGAC 1 cut(s) 594
BsmAI GTCTC 1 cut(s) 730
BsmFI GGGAC 1 cut(s) 594
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 2 cut(s) 902, 948
Bsp1286I GDGCHC 1 cut(s) 72
Bsp143I GATC 4 cut(s) 7, 377, 776, 794
BspACI CCGC 2 cut(s) 49, 677
BspANI GGCC 2 cut(s) 902, 948
BspCNI CTCAG 2 cut(s) 24, 785
BspOI GCTAGC 1 cut(s) 722
BspPI GGATC 3 cut(s) 15, 784, 802
BspQI GCTCTTC 1 cut(s) 398
BspTI CTTAAG 1 cut(s) 31
BsrI ACTGG 1 cut(s) 370
BssECI CCNNGG 1 cut(s) 872
BssMI GATC 4 cut(s) 7, 377, 776, 794
BssNI GRCGYC 1 cut(s) 62
BssT1I CCWWGG 1 cut(s) 872
Bst2UI CCWGG 2 cut(s) 103, 541
Bst4CI ACNGT 7 cut(s) 211, 459, 470, 689, 745, 930, 964
Bst6I CTCTTC 3 cut(s) 398, 624, 706
BstACI GRCGYC 1 cut(s) 62
BstAFI CTTAAG 1 cut(s) 31
BstC8I GCNNGC 2 cut(s) 720, 914
BstDEI CTNAG 2 cut(s) 11, 772
BstF5I GGATG 3 cut(s) 223, 499, 988
BstKTI GATC 4 cut(s) 10, 380, 779, 797
BstMAI GTCTC 1 cut(s) 730
BstMBI GATC 4 cut(s) 7, 377, 776, 794
BstMWI GCNNNNNNNGC 3 cut(s) 157, 614, 674
BstNI CCWGG 2 cut(s) 103, 541
BstSCI CCNGG 2 cut(s) 101, 539
BstV1I GCAGC 1 cut(s) 93
BstX2I RGATCY 3 cut(s) 7, 377, 776
BstXI CCANNNNNNTGG 1 cut(s) 238
BstYI RGATCY 3 cut(s) 7, 377, 776
BsuRI GGCC 2 cut(s) 902, 948
BtgZI GCGATG 1 cut(s) 267
BtrI CACGTC 1 cut(s) 21
BtsCI GGATG 3 cut(s) 223, 499, 988
BtsI GCAGTG 2 cut(s) 494, 705
BtsIMutI CAGTG 3 cut(s) 494, 705, 903
Cac8I GCNNGC 2 cut(s) 720, 914
Cfr13I GGNCC 1 cut(s) 543
CseI GACGC 1 cut(s) 51
Csp6I GTAC 6 cut(s) 170, 492, 741, 804, 926, 931
CviAII CATG 2 cut(s) 798, 979
CviQI GTAC 6 cut(s) 170, 492, 741, 804, 926, 931
DdeI CTNAG 2 cut(s) 11, 772
DpnI GATC 4 cut(s) 9, 379, 778, 796
DpnII GATC 4 cut(s) 7, 377, 776, 794
EaeI YGGCCR 2 cut(s) 900, 946
Eam1104I CTCTTC 3 cut(s) 398, 624, 706
EarI CTCTTC 3 cut(s) 398, 624, 706
Ecl136II GAGCTC 1 cut(s) 70
Eco130I CCWWGG 1 cut(s) 872
Eco24I GRGCYC 1 cut(s) 72
Eco32I GATATC 1 cut(s) 328
Eco47I GGWCC 1 cut(s) 543
Eco53kI GAGCTC 1 cut(s) 70
EcoICRI GAGCTC 1 cut(s) 70
EcoRII CCWGG 2 cut(s) 101, 539
EcoRV GATATC 1 cut(s) 328
EcoT14I CCWWGG 1 cut(s) 872
EcoT38I GRGCYC 1 cut(s) 72
ErhI CCWWGG 1 cut(s) 872
FaeI CATG 2 cut(s) 801, 982
FaiI YATR 7 cut(s) 47, 363, 596, 605, 627, 799, 980
FalI AAGNNNNNCTT 2 cut(s) 852, 884
FaqI GGGAC 1 cut(s) 594
FatI CATG 2 cut(s) 797, 978
Fnu4HI GCNGC 1 cut(s) 107
FokI GGATG 2 cut(s) 230, 486
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 1 cut(s) 107
FspBI CTAG 3 cut(s) 375, 650, 719
GluI GCNGC 1 cut(s) 107
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 2 cut(s) 902, 948
HapII CCGG 2 cut(s) 38, 73
HgaI GACGC 1 cut(s) 51
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 2 cut(s) 801, 982
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HinfI GANTC 4 cut(s) 184, 366, 428, 770
HpaII CCGG 2 cut(s) 38, 73
HphI GGTGA 1 cut(s) 667
Hpy166II GTNNAC 3 cut(s) 494, 769, 926
Hpy188I TCNGA 3 cut(s) 25, 67, 811
Hpy188III TCNNGA 6 cut(s) 188, 375, 425, 473, 486, 774
Hpy8I GTNNAC 3 cut(s) 494, 769, 926
HpyAV CCTTC 4 cut(s) 103, 509, 878, 914
HpyCH4III ACNGT 7 cut(s) 211, 459, 470, 689, 745, 930, 964
HpyCH4IV ACGT 1 cut(s) 20
HpyCH4V TGCA 7 cut(s) 125, 166, 181, 499, 569, 801, 916
HpyF10VI GCNNNNNNNGC 3 cut(s) 157, 614, 674
HpyF3I CTNAG 2 cut(s) 11, 772
HpySE526I ACGT 1 cut(s) 20
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 2 cut(s) 801, 982
Kzo9I GATC 4 cut(s) 7, 377, 776, 794
LguI GCTCTTC 1 cut(s) 398
LmnI GCTCC 3 cut(s) 51, 75, 665
Lsp1109I GCAGC 1 cut(s) 93
LweI GCATC 5 cut(s) 153, 217, 409, 508, 626
MaeI CTAG 3 cut(s) 375, 650, 719
MaeII ACGT 1 cut(s) 20
MaeIII GTNAC 2 cut(s) 211, 731
MalI GATC 4 cut(s) 9, 379, 778, 796
MboI GATC 4 cut(s) 7, 377, 776, 794
MboII GAAGA 4 cut(s) 415, 641, 693, 967
MflI RGATCY 3 cut(s) 7, 377, 776
MhlI GDGCHC 1 cut(s) 72
MlsI TGGCCA 2 cut(s) 902, 948
MluCI AATT 6 cut(s) 356, 386, 476, 813, 826, 905
MluNI TGGCCA 2 cut(s) 902, 948
MlyI GAGTC 1 cut(s) 764
MnlI CCTC 5 cut(s) 313, 482, 651, 656, 981
Mox20I TGGCCA 2 cut(s) 902, 948
MscI TGGCCA 2 cut(s) 902, 948
MseI TTAA 2 cut(s) 32, 414
MslI CAYNNNNRTG 1 cut(s) 236
Msp20I TGGCCA 2 cut(s) 902, 948
MspCI CTTAAG 1 cut(s) 31
MspI CCGG 2 cut(s) 38, 73
MspR9I CCNGG 2 cut(s) 103, 541
Mva1269I GAATGC 1 cut(s) 308
MvaI CCWGG 2 cut(s) 103, 541
MwoI GCNNNNNNNGC 3 cut(s) 157, 614, 674
NdeII GATC 4 cut(s) 7, 377, 776, 794
NheI GCTAGC 1 cut(s) 718
NlaIII CATG 2 cut(s) 801, 982
NmuCI GTSAC 2 cut(s) 211, 731
PciSI GCTCTTC 1 cut(s) 398
PctI GAATGC 1 cut(s) 308
PfeI GAWTC 3 cut(s) 184, 366, 428
PkrI GCNGC 1 cut(s) 108
PleI GAGTC 1 cut(s) 764
PpsI GAGTC 1 cut(s) 764
Psp124BI GAGCTC 1 cut(s) 72
Psp6I CCWGG 2 cut(s) 101, 539
PspGI CCWGG 2 cut(s) 101, 539
PspPI GGNCC 1 cut(s) 543
PsuI RGATCY 3 cut(s) 7, 377, 776
RsaI GTAC 6 cut(s) 171, 493, 742, 805, 927, 932
RsaNI GTAC 6 cut(s) 170, 492, 741, 804, 926, 931
RseI CAYNNNNRTG 1 cut(s) 236
SacI GAGCTC 1 cut(s) 72
SapI GCTCTTC 1 cut(s) 398
SaqAI TTAA 2 cut(s) 32, 414
SatI GCNGC 1 cut(s) 107
Sau3AI GATC 4 cut(s) 7, 377, 776, 794
Sau96I GGNCC 1 cut(s) 543
SchI GAGTC 1 cut(s) 764
ScrFI CCNGG 2 cut(s) 103, 541
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 5 cut(s) 153, 217, 409, 508, 626
SinI GGWCC 1 cut(s) 543
SmiMI CAYNNNNRTG 1 cut(s) 236
SmlI CTYRAG 2 cut(s) 31, 727
SmoI CTYRAG 2 cut(s) 31, 727
Sse9I AATT 6 cut(s) 356, 386, 476, 813, 826, 905
SsiI CCGC 2 cut(s) 49, 677
SspMI CTAG 3 cut(s) 375, 650, 719
SstI GAGCTC 1 cut(s) 72
StyD4I CCNGG 2 cut(s) 101, 539
StyI CCWWGG 1 cut(s) 872
TaaI ACNGT 7 cut(s) 211, 459, 470, 689, 745, 930, 964
TaiI ACGT 1 cut(s) 23
TaqI TCGA 1 cut(s) 426
TasI AATT 6 cut(s) 356, 386, 476, 813, 826, 905
TatI WGTACW 2 cut(s) 169, 803
TfiI GAWTC 3 cut(s) 184, 366, 428
Tru1I TTAA 2 cut(s) 32, 414
Tru9I TTAA 2 cut(s) 32, 414
TscAI CASTG 3 cut(s) 501, 712, 903
TseFI GTSAC 2 cut(s) 211, 731
TseI GCWGC 1 cut(s) 106
Tsp45I GTSAC 2 cut(s) 211, 731
TspDTI ATGAA 3 cut(s) 315, 642, 831
TspGWI ACGGA 2 cut(s) 230, 737
TspRI CASTG 3 cut(s) 501, 712, 903
Vha464I CTTAAG 1 cut(s) 31
VpaK11BI GGWCC 1 cut(s) 543
XapI RAATTY 3 cut(s) 356, 476, 905
XbaI TCTAGA 1 cut(s) 374
XspI CTAG 3 cut(s) 375, 650, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.