MD02G1272700.v1.1

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001760
Physical Location & Seq
Reverse (-)
32703764 .. 32705630
1867 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1272700.v1.1.491

Sequence Viewer

Length: 1335 bp
ATGCTTCAAAGTCTAAAAAGCAAGTATTTAATACCTCTGGCTGATATGTATAAGACGATAACATGTAGGCTCCAGCAGCTCAACTCTGTCTCTCAACAGACAGAAACAGTCGGTATTGAGAAACTTAATGCCTTGAGGAATAGGGTGAAGTCAATCATTTCTAATCTAAATTTGCCCAAAAGCAAAGTTGCACCTTCGTCCAAGGAGAATCTGGGTGTATATGAGAAGTTCATTACTTCTATTCTTAGTGTACGTGGAAGGGGAAATTCTGGTCCCTCGGAGCAGCATGGACAACGTTCTTCTGATGTGAACTCTACCCGTCAATTCATGCAACTTCATTCTCAAACTCCTCCAGTTCAGACACAGCAAGACGAAAAGAAGTCAACATTTCAGTCAGTGAACTCACAGGATTCTGCAACAACAATGCAGCGGAGCAATGCAACAAACTTGGTTAACGATTCTAAGTCTGTATCTGAGTGCCCAACCCTGCAGCCGACCGACACATATTCAGGAAAGATAGGCTCATTTAACATAGTGCAGCAGGTTTCCTCAGGAACCTTAAATATTCCTAAAACCAATCTCCAACAACCAAATGTTAAGACCTTGTTGTCACATAGCCCGGTGCATGGACCACAGTCAAATGTGAATGCTTTTGAGTCAAGCTGTAATACATTTCAAGAGATGCACCTGAAACAACCTAAAGAGGAGCGAGATGTGCAGGCTCAGACCCTGAAGCAAGAACCTCAAGATTGGCATGAGATGCTGCAGCAATCAGTACAAAAGCAAATTGTGCAACAGCATAGCAGGCTGCTAAAGTTTCAATCCGGAGCTTCATTTCCTACTGCTTTGCCCAAAACTCATGGTTCTTCCCATTCACAAGTTCCTCGGTGTTCTACCATTGACAAGAAAAATCTTCTGGTGCCTTTTACAGAAGCAAGAACCACTTTCTATCCCATAAACACACCCTCTACTAAAACTTGTTTGGTTCCCTTGCCTTCATCGTCTGTCCCAGGAGATTTTGAGATGCCTATGTCTGATACTTCATCCCTATCGAATGCTGGAACTATTGGAGATCATATGGTAGATGGTGCACCAAAATCCACATCACCCATTGCCATCAGCACTCCTGGGATATCTGCTTCATTCTTACTTGAAGAATCCAGTAACGACATTCATTGTAATACATCTACAATAATGTCTGATGAGTTGAGTGCTTCTAAGCAGCCTATTCAGCGCTTAGTTAAAGTGGTGAACATGATGTCATCTAAAGCACTAAGTGCTGCTATTTTGGACATTGGCTCTGTCGTTTGTACAACTGATAGGTTATCCTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

445

Amino Acids

48.55

Weight (kDa)

8.95

Isoelectric Point (pI)

59.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 607
Acc36I ACCTGC 1 cut(s) 532
AccB1I GGYRCC 1 cut(s) 919
AccIII TCCGGA 1 cut(s) 824
AciI CCGC 1 cut(s) 430
AclI AACGTT 1 cut(s) 295
AcsI RAATTY 2 cut(s) 169, 265
AcuI CTGAAG 1 cut(s) 752
AdeI CACNNNGTG 1 cut(s) 1277
AfaI GTAC 3 cut(s) 252, 777, 1312
AfeI AGCGCT 1 cut(s) 1235
AfiI CCNNNNNNNGG 1 cut(s) 626
AflIII ACRYGT 1 cut(s) 62
AgsI TTSAA 4 cut(s) 8, 677, 821, 1154
AjnI CCWGG 2 cut(s) 1009, 1126
AluBI AGCT 3 cut(s) 79, 663, 830
AluI AGCT 3 cut(s) 79, 663, 830
Alw21I GWGCWC 1 cut(s) 1093
Alw26I GTCTC 1 cut(s) 94
Alw44I GTGCAC 1 cut(s) 1089
AlwNI CAGNNNCTG 1 cut(s) 730
Aor13HI TCCGGA 1 cut(s) 824
Aor51HI AGCGCT 1 cut(s) 1235
ApaLI GTGCAC 1 cut(s) 1089
ApoI RAATTY 2 cut(s) 169, 265
AspLEI GCGC 1 cut(s) 1236
AspS9I GGNCC 2 cut(s) 272, 629
AsuC2I CCSGG 1 cut(s) 620
AsuHPI GGTGA 3 cut(s) 157, 1098, 1261
AvaII GGWCC 2 cut(s) 272, 629
AxyI CCTNAGG 1 cut(s) 550
BaeGI GKGCMC 2 cut(s) 482, 1093
BanI GGYRCC 1 cut(s) 919
Bbv12I GWGCWC 1 cut(s) 1093
BccI CCATC 2 cut(s) 1079, 1124
BciT130I CCWGG 2 cut(s) 1011, 1128
BcnI CCSGG 1 cut(s) 620
BcoDI GTCTC 1 cut(s) 94
BfmI CTRYAG 2 cut(s) 488, 764
BfoI RGCGCY 1 cut(s) 1237
BfuAI ACCTGC 1 cut(s) 532
Bme1390I CCNGG 3 cut(s) 620, 1011, 1128
Bme18I GGWCC 2 cut(s) 272, 629
BmgT120I GGNCC 2 cut(s) 272, 629
BmiI GGNNCC 5 cut(s) 71, 274, 556, 921, 987
BmrFI CCNGG 3 cut(s) 620, 1011, 1128
BmsI GCATC 3 cut(s) 672, 750, 1014
BoxI GACNNNNGTC 1 cut(s) 634
BpmI CTGGAG 2 cut(s) 56, 336
BpuEI CTTGAG 2 cut(s) 154, 729
BpuMI CCSGG 1 cut(s) 620
BsaAI YACGTR 1 cut(s) 254
BsaJI CCNNGG 5 cut(s) 201, 276, 884, 1009, 1127
BsaWI WCCGGW 1 cut(s) 824
Bsc4I CCNNNNNNNGG 1 cut(s) 626
Bse1I ACTGG 2 cut(s) 353, 1161
Bse21I CCTNAGG 1 cut(s) 550
Bse3DI GCAATG 2 cut(s) 442, 1110
BseAI TCCGGA 1 cut(s) 824
BseBI CCWGG 2 cut(s) 1011, 1128
BseDI CCNNGG 5 cut(s) 201, 276, 884, 1009, 1127
BseGI GGATG 1 cut(s) 1043
BseLI CCNNNNNNNGG 1 cut(s) 626
BseMI GCAATG 2 cut(s) 442, 1110
BseMII CTCAG 3 cut(s) 465, 564, 737
BseNI ACTGG 2 cut(s) 353, 1161
BseRI GAGGAG 2 cut(s) 339, 719
BseSI GKGCMC 2 cut(s) 482, 1093
BsgI GTGCAG 2 cut(s) 557, 737
Bsh1285I CGRYCG 1 cut(s) 498
BshNI GGYRCC 1 cut(s) 919
BsiEI CGRYCG 1 cut(s) 498
BsiHKAI GWGCWC 1 cut(s) 1093
BsiSI CCGG 2 cut(s) 620, 825
BslFI GGGAC 2 cut(s) 258, 992
BslI CCNNNNNNNGG 1 cut(s) 626
BsmAI GTCTC 1 cut(s) 94
BsmFI GGGAC 2 cut(s) 258, 992
BsmI GAATGC 2 cut(s) 652, 1060
Bsp1286I GDGCHC 2 cut(s) 482, 1093
Bsp13I TCCGGA 1 cut(s) 824
Bsp1407I TGTACA 1 cut(s) 1310
Bsp143I GATC 1 cut(s) 1072
BspACI CCGC 1 cut(s) 430
BspCNI CTCAG 3 cut(s) 466, 563, 736
BspEI TCCGGA 1 cut(s) 824
BspLI GGNNCC 5 cut(s) 71, 274, 556, 921, 987
BspMAI CTGCAG 2 cut(s) 492, 768
BspMI ACCTGC 1 cut(s) 532
BspT107I GGYRCC 1 cut(s) 919
BsrDI GCAATG 2 cut(s) 442, 1110
BsrGI TGTACA 1 cut(s) 1310
BsrI ACTGG 2 cut(s) 353, 1161
BssECI CCNNGG 5 cut(s) 201, 276, 884, 1009, 1127
BssMI GATC 1 cut(s) 1072
BssT1I CCWWGG 1 cut(s) 201
Bst2UI CCWGG 2 cut(s) 1011, 1128
Bst4CI ACNGT 2 cut(s) 109, 636
BstAPI GCANNNNNTGC 3 cut(s) 760, 790, 1277
BstAUI TGTACA 1 cut(s) 1310
BstBAI YACGTR 1 cut(s) 254
BstC8I GCNNGC 2 cut(s) 720, 806
BstDEI CTNAG 8 cut(s) 245, 462, 474, 550, 723, 1218, 1237, 1274
BstF5I GGATG 1 cut(s) 1043
BstH2I RGCGCY 1 cut(s) 1237
BstHHI GCGC 1 cut(s) 1236
BstKTI GATC 1 cut(s) 1075
BstMAI GTCTC 1 cut(s) 94
BstMBI GATC 1 cut(s) 1072
BstMCI CGRYCG 1 cut(s) 498
BstMWI GCNNNNNNNGC 7 cut(s) 76, 715, 760, 790, 805, 1231, 1277
BstNI CCWGG 2 cut(s) 1011, 1128
BstNSI RCATGY 1 cut(s) 66
BstPAI GACNNNNGTC 1 cut(s) 634
BstSCI CCNGG 3 cut(s) 618, 1009, 1126
BstSFI CTRYAG 2 cut(s) 488, 764
BstSLI GKGCMC 2 cut(s) 482, 1093
Bsu36I CCTNAGG 1 cut(s) 550
BtsCI GGATG 1 cut(s) 1043
BtsIMutI CAGTG 1 cut(s) 402
BveI ACCTGC 1 cut(s) 532
Cac8I GCNNGC 2 cut(s) 720, 806
CaiI CAGNNNCTG 1 cut(s) 730
CfoI GCGC 1 cut(s) 1236
Cfr13I GGNCC 2 cut(s) 272, 629
Csp6I GTAC 3 cut(s) 251, 776, 1311
CviAII CATG 7 cut(s) 63, 287, 328, 626, 755, 860, 1255
CviQI GTAC 3 cut(s) 251, 776, 1311
DdeI CTNAG 8 cut(s) 245, 462, 474, 550, 723, 1218, 1237, 1274
DpnI GATC 1 cut(s) 1074
DpnII GATC 1 cut(s) 1072
DraIII CACNNNGTG 1 cut(s) 1277
DrdI GACNNNNNNGTC 1 cut(s) 607
DseDI GACNNNNNNGTC 1 cut(s) 607
Eco130I CCWWGG 1 cut(s) 201
Eco32I GATATC 1 cut(s) 1134
Eco47I GGWCC 2 cut(s) 272, 629
Eco47III AGCGCT 1 cut(s) 1235
Eco57I CTGAAG 1 cut(s) 752
Eco81I CCTNAGG 1 cut(s) 550
EcoRII CCWGG 2 cut(s) 1009, 1126
EcoRV GATATC 1 cut(s) 1134
EcoT14I CCWWGG 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 7 cut(s) 66, 290, 331, 629, 758, 863, 1258
FalI AAGNNNNNCTT 2 cut(s) 928, 960
FaqI GGGAC 2 cut(s) 258, 992
FatI CATG 7 cut(s) 62, 286, 327, 625, 754, 859, 1254
FauNDI CATATG 1 cut(s) 1077
FokI GGATG 1 cut(s) 1030
GlaI GCGC 1 cut(s) 1235
GsuI CTGGAG 2 cut(s) 56, 336
HaeII RGCGCY 1 cut(s) 1237
HapII CCGG 2 cut(s) 620, 825
HhaI GCGC 1 cut(s) 1236
Hin1II CATG 7 cut(s) 66, 290, 331, 629, 758, 863, 1258
Hin6I GCGC 1 cut(s) 1234
HinP1I GCGC 1 cut(s) 1234
HincII GTYRAC 2 cut(s) 384, 454
HindII GTYRAC 2 cut(s) 384, 454
HinfI GANTC 5 cut(s) 208, 410, 458, 656, 1157
HpaI GTTAAC 1 cut(s) 454
HpaII CCGG 2 cut(s) 620, 825
HphI GGTGA 3 cut(s) 157, 1098, 1261
Hpy166II GTNNAC 7 cut(s) 251, 310, 384, 400, 454, 1091, 1252
Hpy188I TCNGA 7 cut(s) 280, 304, 360, 475, 726, 1036, 1201
Hpy188III TCNNGA 5 cut(s) 510, 552, 677, 746, 825
Hpy8I GTNNAC 7 cut(s) 251, 310, 384, 400, 454, 1091, 1252
HpyAV CCTTC 3 cut(s) 204, 252, 1005
HpyCH4III ACNGT 2 cut(s) 109, 636
HpyCH4IV ACGT 2 cut(s) 253, 295
HpyF10VI GCNNNNNNNGC 7 cut(s) 76, 715, 760, 790, 805, 1231, 1277
HpyF3I CTNAG 8 cut(s) 245, 462, 474, 550, 723, 1218, 1237, 1274
HpySE526I ACGT 2 cut(s) 253, 295
Hsp92II CATG 7 cut(s) 66, 290, 331, 629, 758, 863, 1258
HspAI GCGC 1 cut(s) 1234
Kpn2I TCCGGA 1 cut(s) 824
KspAI GTTAAC 1 cut(s) 454
Kzo9I GATC 1 cut(s) 1072
LmnI GCTCC 5 cut(s) 75, 280, 432, 706, 827
LweI GCATC 3 cut(s) 672, 750, 1014
MaeII ACGT 2 cut(s) 253, 295
MaeIII GTNAC 2 cut(s) 609, 1163
MalI GATC 1 cut(s) 1074
MboI GATC 1 cut(s) 1072
MboII GAAGA 4 cut(s) 291, 858, 905, 1166
MhlI GDGCHC 2 cut(s) 482, 1093
MluCI AATT 4 cut(s) 169, 265, 323, 786
MlyI GAGTC 1 cut(s) 665
MmeI TCCRAC 1 cut(s) 607
MnlI CCTC 9 cut(s) 45, 129, 286, 360, 559, 697, 753, 894, 976
MroI TCCGGA 1 cut(s) 824
MseI TTAA 7 cut(s) 29, 126, 453, 528, 560, 597, 1242
MspA1I CMGCKG 1 cut(s) 430
MspI CCGG 2 cut(s) 620, 825
MspR9I CCNGG 3 cut(s) 620, 1011, 1128
Mva1269I GAATGC 2 cut(s) 652, 1060
MvaI CCWGG 2 cut(s) 1011, 1128
MwoI GCNNNNNNNGC 7 cut(s) 76, 715, 760, 790, 805, 1231, 1277
NciI CCSGG 1 cut(s) 620
NdeI CATATG 1 cut(s) 1077
NdeII GATC 1 cut(s) 1072
NlaIII CATG 7 cut(s) 66, 290, 331, 629, 758, 863, 1258
NlaIV GGNNCC 5 cut(s) 71, 274, 556, 921, 987
NmuCI GTSAC 1 cut(s) 609
NspI RCATGY 1 cut(s) 66
PciI ACATGT 1 cut(s) 62
PctI GAATGC 2 cut(s) 652, 1060
PfeI GAWTC 4 cut(s) 208, 410, 458, 1157
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
Ppu21I YACGTR 1 cut(s) 254
PscI ACATGT 1 cut(s) 62
PshAI GACNNNNGTC 1 cut(s) 634
Psp1406I AACGTT 1 cut(s) 295
Psp6I CCWGG 2 cut(s) 1009, 1126
PspGI CCWGG 2 cut(s) 1009, 1126
PspN4I GGNNCC 5 cut(s) 71, 274, 556, 921, 987
PspPI GGNCC 2 cut(s) 272, 629
PstI CTGCAG 2 cut(s) 492, 768
PstNI CAGNNNCTG 1 cut(s) 730
RsaI GTAC 3 cut(s) 252, 777, 1312
RsaNI GTAC 3 cut(s) 251, 776, 1311
SaqAI TTAA 7 cut(s) 29, 126, 453, 528, 560, 597, 1242
Sau3AI GATC 1 cut(s) 1072
Sau96I GGNCC 2 cut(s) 272, 629
SchI GAGTC 1 cut(s) 665
ScrFI CCNGG 3 cut(s) 620, 1011, 1128
SduI GDGCHC 2 cut(s) 482, 1093
SfaNI GCATC 3 cut(s) 672, 750, 1014
SfcI CTRYAG 2 cut(s) 488, 764
SinI GGWCC 2 cut(s) 272, 629
SmlI CTYRAG 2 cut(s) 133, 744
SmoI CTYRAG 2 cut(s) 133, 744
Sse9I AATT 4 cut(s) 169, 265, 323, 786
SsiI CCGC 1 cut(s) 430
SspI AATATT 1 cut(s) 565
StyD4I CCNGG 3 cut(s) 618, 1009, 1126
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 2 cut(s) 109, 636
TaiI ACGT 2 cut(s) 256, 298
TaqI TCGA 1 cut(s) 1052
TaqII GACCGA 1 cut(s) 512
TasI AATT 4 cut(s) 169, 265, 323, 786
TatI WGTACW 2 cut(s) 775, 1310
TfiI GAWTC 4 cut(s) 208, 410, 458, 1157
Tru1I TTAA 7 cut(s) 29, 126, 453, 528, 560, 597, 1242
Tru9I TTAA 7 cut(s) 29, 126, 453, 528, 560, 597, 1242
TscAI CASTG 1 cut(s) 402
TseFI GTSAC 1 cut(s) 609
Tsp45I GTSAC 1 cut(s) 609
TspDTI ATGAA 8 cut(s) 220, 316, 326, 822, 987, 1032, 1131, 1163
TspRI CASTG 1 cut(s) 402
VneI GTGCAC 1 cut(s) 1089
VpaK11BI GGWCC 2 cut(s) 272, 629
XapI RAATTY 2 cut(s) 169, 265
XceI RCATGY 1 cut(s) 66
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.