MD07G1041400.v1.1

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001760
Physical Location & Seq
Reverse (-)
3442941 .. 3451868
8928 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1041400.v1.1.491

Sequence Viewer

Length: 3516 bp
ATGGATCCAGTCAATTGGAGAACTAAATTTCCTGCAGAGTCTCGAGAAAAAGTTGTGAACAAGATTATAAGTAGCTTAATGAAGCGTTTTCCATATTCTGAGCACGAGAAACATATGTCTGATTACAAGAGGATAGCTGTAAATTTCGAGGAAAAGGTTTATATGGTTGCCAGAGACCAGCAAGACTATATAATGCGGATCAAATCGAGGTTGGAGTCAAAAAACTTGAATCCAGGTGGTAAGCAAGGCCAATCGATTTCTGTTCAAGCCACAACTCTGTCCCAAGCACATGAACAACATTCATTGCCTAATGTTCAAGATAACAATGCATCTGGTGGAGTCGTTACTAATTTAGCATCTGCACGGCCCACTGTCAGTATTTCGCCTCACGTTGCTTTGCCAAACAATGCTAGTGAAAATTCCAATTTGCAGAGTGTGTTTGGTAATTCACAAAGGTCTGTAGGAAATTTATCAGGGTGTGGCCAACCTTTTAATATTTCTGTCAATAATTTGAGACAGGAGCAAGGGAGACAAAATTCACCCCAGATTGTTTCTCAGCAGAACCATGAGCAGCTAAATCCTCATCTTTATAAGCAGGTAAAGCAGGAAAATATCTCACCGTCATATATAACCCAGAAGCAACTACAGCCGCAGCACCACTACCAACCGAAACTCTTAAACCCTACTCAGCAACACACCTCTCAAAAATCTATGTTGCAAACTCCTGTCATGTGCCCTTCGGAGACACGCGTTGCTCAACTTTCTGATATTTGGCAGGATCAGATGTCTTATTTTGATCAGACAACACAGTCGGGGAATAAGACTCAGTCACAGATAGCCTTTAGGCAAACAAACCAATCACAAGTGGCTCTGGATATTCATCAAAATGTGTCATCAAGTTCACAGAACCCATTAATGCAATCACAAAAGCAAGTACATTTGATAGGGAGAAAGTTGACTGCTACTAATACGCAACAGAGCCACTTAGATGGGCAACCAAATGGCTGCCCTAACTTGCACCAGCAACAGCCCAGGTTACCATGCCAACAGAATACTGTACAACAGCAGTTTCAGGTACAGAAAATAAATCATTCAAGTGCACATCATGAGCAATTGAGTGAAAGAAACAATATGTATGTACTGCCACAAAGCTCACAGCTGCTTGAAACAGAGTGTGCCAGCTCAGGCATGCGCCTATGCGAGGTTAAAATGCAGGGGCAAACCCAACATAAAGCTTCAGTTCTGCTGCCAAATCAAGATCAAGAATCACAAGCAGATTCTCTTGAGAGACGACTGAACTCACAAATACAGCCACAGAAGATGGAACAACTGCCAGATCCATCAGCACAAGATCAGCAGCAAACATATCAAACATCAGGCACACTAACTTCTTCATCTGCGAGAAGGGTTTCATGCACAGAAAACTGGCAAGAGGATGCCTACCAGATGCTTCAAAGTCTAAAAAGCAAGTATTTAACACCTCTGACTGATATGTATAATACAATAACGTGTAGGCTCCAGCAGCTCAACTCTATCTCTCAACAGACAGAAACAGGCAGCATTGAGAAACTTAATTCATTGAGGAATAGGGTGAATACAATCATTTCTAATCTAAGTTTTCCCAAAAGCAATATTCCACCTTCTTACAAGGAGAGTCTGGGTTTATTTGAGAAGTACATTATTTCTATTCTTAGTGTACGTGGAAGGGGAAATTCTGCTCCCTCACAGCAGCATGGACAACAATCTTCTGATGTGCACCCTATGCGTCAATTCGTACAACTTCGTTCTCAAACTTCTCCAGTTCAGACACAGCAAGACGAAAAGAAGCCAACATTTCAGCCTGTGAACTCACAGGATTCTGCAACAATGCAGCGGAGCAATGCAACGAACTTGGAAAACGATTCTAAGTCTGTATCTGAGCACCCAACCCTGCAGCCGACTGAGAAGTATTCAGGAAAGATAGGCTCATTTAACATAGTGCAGCAGGTTTCCTCAGGAACCTTAAATATTCCTAGAAGCAATCTCCAACAACCAAACATTAAGGCCTTGTTGTCACATAGCCCCGTGCATGGACCACAGTCAAACGTGAATGCTTTTGAGCCAAGCTGTAATACACTTCAAGAGATGTGCTTGAAACAACCTAAAGAAGAGCGAGATGTGCAGACTCAGACCCCAAAGCAAGAACTTCAAGATAGGCATGAGATGCTGCACCAATCAGTACAACAAAAACAAATTATGCAACAGCATAGTGGGATGGTAAGGTTGCAATCTGGAGCTTCATTTCCTACTGCTTTGCCCAAAACTTATGGTTCTTCCTGCTCACAAGTTCCTCGGTGTCCTACTATTGACAAGAAAAATCTTCCGATGCCTCTAACAGAAGCTAGAACGACTTTCTATCCCATAAACTCGTGCTCTACTAAAACTTCTTGTTCGGTTCCCTTGCCTTCATCATCTGTCCCGGGAGATTTAGAGAGACCTATTTCTGATACTTCATCCCTATCAAATGCTGGAGATGAACAAGTAAATATTGCAGCAAAATCCCCATCACCCATTGCCATCAGTACTCCTGGGATATCTGTTTCACCCTTACTTGAAGAGTCCAGTAACGTCATTCATTGTGATACATCTACAACAATGTCTGATGAGTTGAGAGCTTCTGAGCAGCCTATTCAGCGCTTAATTAAAGTGGTGAACTTGATGTCATCTAAAGCACTAAGTGCTTCTGTTTTGGACATTGGCTCTGTAGTTTGTACAACTGATAGGATATCAGGGTCAGCAACACTTACGGGATCTGGAGGTTCAATAGGTGAGGATTTAGCGGGCATGACCAATTATAGTTTGCAGAAGAGTTATTTAACATGGCAAGGTATGACCTTTGGAACAAAAAATATGAAACGCTGCAGAAGTACAGTGCCAATTGATGTCGTTGCGACAGCAACTGGAAGTATCTGTGATAGTTCAAAGTTAAGTGATACGGAGATATTTGACTTGGAGTCAACTGCAATATCTTATATCAAAAGGCCAAGGATTGAGGTGAATCAAACCCTGTTAGAAGAAATTACAGCGATCAACTACCAACTAATAGATACTGTACTAGATATCAGTGATGAAGATACCCCTCCGACAGTAGTCTCAGCTCAATTTAGAGGTGGTCAAGGAACCATTGTCCGGTGCTCCTTCATTGCCGTGTCCCTCAGTCCCAATCTGAAGTCACAGCAGTTTCCCATCCAGCCTTTACGATTGCTTGTTCCTGCAAATTATCCATTGTGCTCTCCCATATTTCTAGACAAGCTGAGAGTGGAAGTCAGTGATGAGCTAGAAGATCCATCAGAAAAGGTAAAATCAAAGCTGAATATGTCTCTCCGGAGGCTGACAGAACCCTTGTCACTTGGGGAGATAGCAAGGACGTGGGATGTTTGTGCGCGAGCCGTTGTCTCCGAGTATGCGCAGCAGAATGGGGGAGGAAACTTGAGTTCCAAGTATGGAGAGTGGGAAGACTGTCTCAATGCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

1172

Amino Acids

130.01

Weight (kDa)

7.93

Isoelectric Point (pI)

63.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 5 - 72 5e-18 KIX domain
Med15_C PF21539 1064 - 1142 2.9e-06 ARC105/Med15 mediator subunit C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 68, 591
AasI GACNNNNNNGTC 1 cut(s) 808
Acc16I TGCGCA 1 cut(s) 3447
Acc36I ACCTGC 2 cut(s) 586, 1975
AccII CGCG 2 cut(s) 750, 3424
AccIII TCCGGA 1 cut(s) 3363
AciI CCGC 4 cut(s) 196, 650, 1873, 2819
AclWI GGATC 6 cut(s) 12, 206, 786, 1331, 2797, 3317
AcoI YGGCCR 1 cut(s) 481
AcsI RAATTY 6 cut(s) 26, 142, 418, 466, 535, 1711
AcuI CTGAAG 2 cut(s) 1221, 3227
AdeI CACNNNGTG 1 cut(s) 2717
AfeI AGCGCT 1 cut(s) 2675
AfiI CCNNNNNNNGG 3 cut(s) 1201, 2069, 3168
AflIII ACRYGT 2 cut(s) 748, 1508
AhdI GACNNNNNGTC 1 cut(s) 2992
AjiI CACGTC 1 cut(s) 3408
AjnI CCWGG 3 cut(s) 232, 1031, 2566
AloI GAACNNNNNNTCC 2 cut(s) 13, 45
Alw21I GWGCWC 7 cut(s) 105, 1102, 1758, 1923, 2414, 3176, 3272
Alw44I GTGCAC 2 cut(s) 1098, 1754
AlwI GGATC 6 cut(s) 12, 206, 786, 1331, 2797, 3317
AlwNI CAGNNNCTG 1 cut(s) 2939
Ama87I CYCGRG 2 cut(s) 42, 2458
Aor13HI TCCGGA 1 cut(s) 3363
Aor51HI AGCGCT 1 cut(s) 2675
AoxI GGCC 5 cut(s) 247, 365, 481, 2043, 3020
ApaLI GTGCAC 2 cut(s) 1098, 1754
ApoI RAATTY 6 cut(s) 26, 142, 418, 466, 535, 1711
AseI ATTAAT 1 cut(s) 914
Asp700I GAANNNNTTC 1 cut(s) 1408
AspLEI GCGC 4 cut(s) 1194, 2676, 3424, 3448
AspS9I GGNCC 2 cut(s) 366, 2072
AsuC2I CCSGG 2 cut(s) 2459, 2460
AsuHPI GGTGA 8 cut(s) 531, 609, 1603, 2538, 2574, 2701, 2819, 3046
AvaI CYCGRG 2 cut(s) 42, 2458
AvaII GGWCC 1 cut(s) 2072
AxyI CCTNAGG 1 cut(s) 1993
BaeGI GKGCMC 3 cut(s) 737, 1102, 1758
BalI TGGCCA 1 cut(s) 483
BamHI GGATCC 1 cut(s) 4
BauI CACGAG 2 cut(s) 104, 2407
BbsI GAAGAC 1 cut(s) 3501
Bbv12I GWGCWC 7 cut(s) 105, 1102, 1758, 1923, 2414, 3176, 3272
BccI CCATC 8 cut(s) 983, 1315, 1348, 2248, 2551, 2564, 3233, 3334
BceAI ACGGC 3 cut(s) 380, 3170, 3413
BciT130I CCWGG 3 cut(s) 234, 1033, 2568
BclI TGATCA 1 cut(s) 796
BcnI CCSGG 2 cut(s) 2459, 2460
BfaI CTAG 6 cut(s) 411, 2013, 2382, 3095, 3284, 3317
BfmI CTRYAG 6 cut(s) 33, 459, 644, 1931, 2742, 2899
BfoI RGCGCY 1 cut(s) 2677
BfuAI ACCTGC 2 cut(s) 586, 1975
BmcAI AGTACT 1 cut(s) 2563
Bme1390I CCNGG 5 cut(s) 234, 1033, 2459, 2460, 2568
Bme18I GGWCC 1 cut(s) 2072
BmeRI GACNNNNNGTC 1 cut(s) 2992
BmeT110I CYCGRG 2 cut(s) 42, 2458
BmgBI CACGTC 1 cut(s) 3408
BmgT120I GGNCC 2 cut(s) 366, 2072
BmiI GGNNCC 5 cut(s) 6, 1517, 1999, 2436, 3160
BmrFI CCNGG 5 cut(s) 234, 1033, 2459, 2460, 2568
BmsI GCATC 6 cut(s) 338, 365, 1426, 1437, 2193, 2355
BoxI GACNNNNGTC 2 cut(s) 2077, 3128
BpiI GAAGAC 1 cut(s) 3501
BpmI CTGGAG 5 cut(s) 1502, 1782, 2292, 2529, 2814
Bpu10I CCTNAGC 1 cut(s) 1183
BpuEI CTTGAG 2 cut(s) 1304, 3490
BpuMI CCSGG 2 cut(s) 2459, 2460
Bsa29I ATCGAT 1 cut(s) 254
BsaAI YACGTR 1 cut(s) 1700
BsaBI GATNNNNATC 1 cut(s) 879
BsaI GGTCTC 2 cut(s) 168, 2467
BsaJI CCNNGG 5 cut(s) 1031, 2330, 2458, 2567, 3023
BsaWI WCCGGW 2 cut(s) 3168, 3363
Bsc4I CCNNNNNNNGG 3 cut(s) 1201, 2069, 3168
Bse1I ACTGG 5 cut(s) 8, 1430, 1799, 2601, 2944
Bse21I CCTNAGG 1 cut(s) 1993
Bse3DI GCAATG 4 cut(s) 302, 1885, 2550, 3180
Bse8I GATNNNNATC 1 cut(s) 879
BseAI TCCGGA 1 cut(s) 3363
BseBI CCWGG 3 cut(s) 234, 1033, 2568
BseCI ATCGAT 1 cut(s) 254
BseDI CCNNGG 5 cut(s) 1031, 2330, 2458, 2567, 3023
BseGI GGATG 5 cut(s) 1441, 2259, 2492, 3225, 3418
BseJI GATNNNNATC 1 cut(s) 879
BseLI CCNNNNNNNGG 3 cut(s) 1201, 2069, 3168
BseMI GCAATG 4 cut(s) 302, 1885, 2550, 3180
BseNI ACTGG 5 cut(s) 8, 1430, 1799, 2601, 2944
BseSI GKGCMC 3 cut(s) 737, 1102, 1758
BsgI GTGCAG 4 cut(s) 345, 2000, 2180, 2192
Bsh1236I CGCG 2 cut(s) 750, 3424
BshFI GGCC 5 cut(s) 249, 367, 483, 2045, 3022
BshVI ATCGAT 1 cut(s) 254
BsiHKAI GWGCWC 7 cut(s) 105, 1102, 1758, 1923, 2414, 3176, 3272
BsiHKCI CYCGRG 2 cut(s) 42, 2458
BsiSI CCGG 3 cut(s) 2459, 3169, 3364
BslFI GGGAC 4 cut(s) 265, 2441, 3175, 3183
BslI CCNNNNNNNGG 3 cut(s) 1201, 2069, 3168
BsmBI CGTCTC 1 cut(s) 1282
BsmFI GGGAC 4 cut(s) 265, 2441, 3175, 3183
BsmI GAATGC 1 cut(s) 2095
BsnI GGCC 5 cut(s) 249, 367, 483, 2045, 3022
Bso31I GGTCTC 2 cut(s) 168, 2467
BsoBI CYCGRG 2 cut(s) 42, 2458
Bsp1286I GDGCHC 8 cut(s) 105, 737, 1102, 1758, 1923, 2414, 3176, 3272
Bsp13I TCCGGA 1 cut(s) 3363
Bsp1407I TGTACA 2 cut(s) 1057, 2750
BspACI CCGC 4 cut(s) 196, 650, 1873, 2819
BspANI GGCC 5 cut(s) 249, 367, 483, 2045, 3022
BspDI ATCGAT 1 cut(s) 254
BspEI TCCGGA 1 cut(s) 3363
BspFNI CGCG 2 cut(s) 750, 3424
BspHI TCATGA 1 cut(s) 1105
BspLI GGNNCC 5 cut(s) 6, 1517, 1999, 2436, 3160
BspMAI CTGCAG 3 cut(s) 37, 1935, 2903
BspMI ACCTGC 2 cut(s) 586, 1975
BspPI GGATC 6 cut(s) 12, 206, 786, 1331, 2797, 3317
BspQI GCTCTTC 1 cut(s) 2142
BspTNI GGTCTC 2 cut(s) 168, 2467
BsrDI GCAATG 4 cut(s) 302, 1885, 2550, 3180
BsrGI TGTACA 2 cut(s) 1057, 2750
BsrI ACTGG 5 cut(s) 8, 1430, 1799, 2601, 2944
BssECI CCNNGG 5 cut(s) 1031, 2330, 2458, 2567, 3023
BssSI CACGAG 2 cut(s) 104, 2407
BssT1I CCWWGG 1 cut(s) 3023
Bst2BI CACGAG 2 cut(s) 104, 2407
Bst2UI CCWGG 3 cut(s) 234, 1033, 2568
Bst4CI ACNGT 9 cut(s) 373, 621, 810, 1057, 2079, 2911, 3091, 3127, 3500
Bst6I CTCTTC 3 cut(s) 2142, 2589, 2840
BstAPI GCANNNNNTGC 3 cut(s) 1762, 2203, 2717
BstAUI TGTACA 2 cut(s) 1057, 2750
BstBAI YACGTR 1 cut(s) 1700
BstC8I GCNNGC 4 cut(s) 1180, 1190, 2821, 3426
BstEII GGTNACC 1 cut(s) 1035
BstENI CCTNNNNNAGG 1 cut(s) 1199
BstF5I GGATG 5 cut(s) 1441, 2259, 2492, 3225, 3418
BstFNI CGCG 2 cut(s) 750, 3424
BstH2I RGCGCY 1 cut(s) 2677
BstHHI GCGC 4 cut(s) 1194, 2676, 3424, 3448
BstMWI GCNNNNNNNGC 8 cut(s) 601, 646, 1522, 1762, 2158, 2203, 2671, 2717
BstNI CCWGG 3 cut(s) 234, 1033, 2568
BstNSI RCATGY 1 cut(s) 1192
BstPAI GACNNNNGTC 2 cut(s) 2077, 3128
BstPI GGTNACC 1 cut(s) 1035
BstSCI CCNGG 5 cut(s) 232, 1031, 2457, 2458, 2566
BstSFI CTRYAG 6 cut(s) 33, 459, 644, 1931, 2742, 2899
BstSLI GKGCMC 3 cut(s) 737, 1102, 1758
BstUI CGCG 2 cut(s) 750, 3424
BstV2I GAAGAC 1 cut(s) 3501
BstX2I RGATCY 4 cut(s) 4, 1336, 2789, 3322
BstXI CCANNNNNNTGG 2 cut(s) 15, 989
BstYI RGATCY 4 cut(s) 4, 1336, 2789, 3322
Bsu15I ATCGAT 1 cut(s) 254
Bsu36I CCTNAGG 1 cut(s) 1993
BsuRI GGCC 5 cut(s) 249, 367, 483, 2045, 3022
BsuTUI ATCGAT 1 cut(s) 254
BtrI CACGTC 1 cut(s) 3408
BtsCI GGATG 5 cut(s) 1441, 2259, 2492, 3225, 3418
BtsIMutI CAGTG 4 cut(s) 369, 2916, 3109, 3313
BveI ACCTGC 2 cut(s) 586, 1975
Cac8I GCNNGC 4 cut(s) 1180, 1190, 2821, 3426
CaiI CAGNNNCTG 1 cut(s) 2939
CciI TCATGA 1 cut(s) 1105
CfoI GCGC 4 cut(s) 1194, 2676, 3424, 3448
Cfr13I GGNCC 2 cut(s) 366, 2072
Cfr9I CCCGGG 1 cut(s) 2458
ClaI ATCGAT 1 cut(s) 254
CseI GACGC 1 cut(s) 1754
DraIII CACNNNGTG 1 cut(s) 2717
DrdI GACNNNNNNGTC 1 cut(s) 808
DriI GACNNNNNGTC 1 cut(s) 2992
DseDI GACNNNNNNGTC 1 cut(s) 808
EaeI YGGCCR 1 cut(s) 481
Eam1104I CTCTTC 3 cut(s) 2142, 2589, 2840
Eam1105I GACNNNNNGTC 1 cut(s) 2992
EarI CTCTTC 3 cut(s) 2142, 2589, 2840
Eco130I CCWWGG 1 cut(s) 3023
Eco147I AGGCCT 1 cut(s) 2045
Eco31I GGTCTC 2 cut(s) 168, 2467
Eco32I GATATC 3 cut(s) 2574, 2766, 3100
Eco47I GGWCC 1 cut(s) 2072
Eco47III AGCGCT 1 cut(s) 2675
Eco57I CTGAAG 2 cut(s) 1221, 3227
Eco81I CCTNAGG 1 cut(s) 1993
Eco88I CYCGRG 2 cut(s) 42, 2458
Eco91I GGTNACC 1 cut(s) 1035
EcoNI CCTNNNNNAGG 1 cut(s) 1199
EcoO65I GGTNACC 1 cut(s) 1035
EcoRII CCWGG 3 cut(s) 232, 1031, 2566
EcoRV GATATC 3 cut(s) 2574, 2766, 3100
EcoT14I CCWWGG 1 cut(s) 3023
EcoT22I ATGCAT 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 3023
Esp3I CGTCTC 1 cut(s) 1282
FaqI GGGAC 4 cut(s) 265, 2441, 3175, 3183
FauI CCCGC 1 cut(s) 2812
FauNDI CATATG 1 cut(s) 114
FbaI TGATCA 1 cut(s) 796
FokI GGATG 5 cut(s) 1448, 2266, 2479, 3212, 3425
FspBI CTAG 6 cut(s) 411, 2013, 2382, 3095, 3284, 3317
FspI TGCGCA 1 cut(s) 3447
GlaI GCGC 4 cut(s) 1193, 2675, 3423, 3447
GsuI CTGGAG 5 cut(s) 1502, 1782, 2292, 2529, 2814
HaeII RGCGCY 1 cut(s) 2677
HaeIII GGCC 5 cut(s) 249, 367, 483, 2045, 3022
HapII CCGG 3 cut(s) 2459, 3169, 3364
HgaI GACGC 1 cut(s) 1754
HhaI GCGC 4 cut(s) 1194, 2676, 3424, 3448
Hin6I GCGC 4 cut(s) 1192, 2674, 3422, 3446
HinP1I GCGC 4 cut(s) 1192, 2674, 3422, 3446
HincII GTYRAC 2 cut(s) 957, 2997
HindII GTYRAC 2 cut(s) 957, 2997
HindIII AAGCTT 1 cut(s) 1233
HpaII CCGG 3 cut(s) 2459, 3169, 3364
HphI GGTGA 8 cut(s) 531, 609, 1603, 2538, 2574, 2701, 2819, 3046
Hpy166II GTNNAC 9 cut(s) 58, 902, 957, 1100, 1697, 1756, 1846, 2692, 2997
Hpy8I GTNNAC 9 cut(s) 58, 902, 957, 1100, 1697, 1756, 1846, 2692, 2997
HpyAV CCTTC 6 cut(s) 747, 1398, 1650, 1698, 2454, 3187
HpyCH4III ACNGT 9 cut(s) 373, 621, 810, 1057, 2079, 2911, 3091, 3127, 3500
HpyCH4IV ACGT 6 cut(s) 390, 1508, 1699, 2085, 2607, 3407
HpyF10VI GCNNNNNNNGC 8 cut(s) 601, 646, 1522, 1762, 2158, 2203, 2671, 2717
HpySE526I ACGT 6 cut(s) 390, 1508, 1699, 2085, 2607, 3407
HspAI GCGC 4 cut(s) 1192, 2674, 3422, 3446
Kpn2I TCCGGA 1 cut(s) 3363
Ksp22I TGATCA 1 cut(s) 796
LguI GCTCTTC 1 cut(s) 2142
LmnI GCTCC 6 cut(s) 520, 1521, 1723, 1875, 2273, 3179
LweI GCATC 6 cut(s) 338, 365, 1426, 1437, 2193, 2355
MaeI CTAG 6 cut(s) 411, 2013, 2382, 3095, 3284, 3317
MaeII ACGT 6 cut(s) 390, 1508, 1699, 2085, 2607, 3407
MaeIII GTNAC 7 cut(s) 343, 828, 1035, 2052, 2603, 3210, 3384
MfeI CAATTG 3 cut(s) 13, 1112, 2916
MflI RGATCY 4 cut(s) 4, 1336, 2789, 3322
MhlI GDGCHC 8 cut(s) 105, 737, 1102, 1758, 1923, 2414, 3176, 3272
MlsI TGGCCA 1 cut(s) 483
MluI ACGCGT 1 cut(s) 748
MluNI TGGCCA 1 cut(s) 483
MlyI GAGTC 8 cut(s) 47, 224, 348, 817, 1663, 2158, 2606, 3002
MmeI TCCRAC 3 cut(s) 192, 2050, 3146
Mox20I TGGCCA 1 cut(s) 483
Mph1103I ATGCAT 1 cut(s) 331
MroI TCCGGA 1 cut(s) 3363
MroXI GAANNNNTTC 1 cut(s) 1408
MscI TGGCCA 1 cut(s) 483
MslI CAYNNNNRTG 4 cut(s) 885, 987, 1095, 3185
Msp20I TGGCCA 1 cut(s) 483
MspA1I CMGCKG 2 cut(s) 1159, 1873
MspI CCGG 3 cut(s) 2459, 3169, 3364
MspR9I CCNGG 5 cut(s) 234, 1033, 2459, 2460, 2568
MunI CAATTG 3 cut(s) 13, 1112, 2916
Mva1269I GAATGC 1 cut(s) 2095
MvaI CCWGG 3 cut(s) 234, 1033, 2568
MvnI CGCG 2 cut(s) 750, 3424
MwoI GCNNNNNNNGC 8 cut(s) 601, 646, 1522, 1762, 2158, 2203, 2671, 2717
NciI CCSGG 2 cut(s) 2459, 2460
NdeI CATATG 1 cut(s) 114
NlaIV GGNNCC 5 cut(s) 6, 1517, 1999, 2436, 3160
NmuCI GTSAC 4 cut(s) 828, 2052, 3210, 3384
NsbI TGCGCA 1 cut(s) 3447
NsiI ATGCAT 1 cut(s) 331
NspI RCATGY 1 cut(s) 1192
PacI TTAATTAA 1 cut(s) 2682
PaeI GCATGC 1 cut(s) 1192
PaeR7I CTCGAG 1 cut(s) 42
PagI TCATGA 1 cut(s) 1105
PceI AGGCCT 1 cut(s) 2045
PciSI GCTCTTC 1 cut(s) 2142
PctI GAATGC 1 cut(s) 2095
PdmI GAANNNNTTC 1 cut(s) 1408
PfeI GAWTC 6 cut(s) 229, 1265, 1277, 1856, 1901, 3037
PflFI GACNNNGTC 1 cut(s) 826
PleI GAGTC 8 cut(s) 46, 223, 347, 817, 1662, 2158, 2605, 3001
PpsI GAGTC 8 cut(s) 46, 223, 347, 817, 1662, 2158, 2605, 3001
Ppu21I YACGTR 1 cut(s) 1700
PshAI GACNNNNGTC 2 cut(s) 2077, 3128
PshBI ATTAAT 1 cut(s) 914
PsiI TTATAA 2 cut(s) 68, 591
Psp6I CCWGG 3 cut(s) 232, 1031, 2566
PspEI GGTNACC 1 cut(s) 1035
PspGI CCWGG 3 cut(s) 232, 1031, 2566
PspN4I GGNNCC 5 cut(s) 6, 1517, 1999, 2436, 3160
PspPI GGNCC 2 cut(s) 366, 2072
PstI CTGCAG 3 cut(s) 37, 1935, 2903
PstNI CAGNNNCTG 1 cut(s) 2939
PsuI RGATCY 4 cut(s) 4, 1336, 2789, 3322
PsyI GACNNNGTC 1 cut(s) 826
PvuII CAGCTG 1 cut(s) 1159
RseI CAYNNNNRTG 4 cut(s) 885, 987, 1095, 3185
SapI GCTCTTC 1 cut(s) 2142
Sau96I GGNCC 2 cut(s) 366, 2072
ScaI AGTACT 1 cut(s) 2563
SchI GAGTC 8 cut(s) 47, 224, 348, 817, 1663, 2158, 2606, 3002
ScrFI CCNGG 5 cut(s) 234, 1033, 2459, 2460, 2568
SduI GDGCHC 8 cut(s) 105, 737, 1102, 1758, 1923, 2414, 3176, 3272
SfaNI GCATC 6 cut(s) 338, 365, 1426, 1437, 2193, 2355
SfcI CTRYAG 6 cut(s) 33, 459, 644, 1931, 2742, 2899
Sfr274I CTCGAG 1 cut(s) 42
SinI GGWCC 1 cut(s) 2072
SlaI CTCGAG 1 cut(s) 42
SmaI CCCGGG 1 cut(s) 2460
SmiMI CAYNNNNRTG 4 cut(s) 885, 987, 1095, 3185
SmlI CTYRAG 3 cut(s) 42, 1283, 3469
SmoI CTYRAG 3 cut(s) 42, 1283, 3469
SphI GCATGC 1 cut(s) 1192
SseBI AGGCCT 1 cut(s) 2045
SsiI CCGC 4 cut(s) 196, 650, 1873, 2819
SspI AATATT 4 cut(s) 496, 1633, 2008, 2527
SspMI CTAG 6 cut(s) 411, 2013, 2382, 3095, 3284, 3317
StuI AGGCCT 1 cut(s) 2045
StyD4I CCNGG 5 cut(s) 232, 1031, 2457, 2458, 2566
StyI CCWWGG 1 cut(s) 3023
TaaI ACNGT 9 cut(s) 373, 621, 810, 1057, 2079, 2911, 3091, 3127, 3500
TaiI ACGT 6 cut(s) 393, 1511, 1702, 2088, 2610, 3410
TaqI TCGA 4 cut(s) 43, 147, 206, 254
TatI WGTACW 9 cut(s) 934, 1057, 1138, 1674, 2218, 2561, 2750, 2906, 3091
TauI GCSGC 1 cut(s) 652
TfiI GAWTC 6 cut(s) 229, 1265, 1277, 1856, 1901, 3037
TscAI CASTG 4 cut(s) 376, 2916, 3109, 3313
TseFI GTSAC 4 cut(s) 828, 2052, 3210, 3384
Tsp45I GTSAC 4 cut(s) 828, 2052, 3210, 3384
TspGWI ACGGA 1 cut(s) 2990
TspMI CCCGGG 1 cut(s) 2458
TspRI CASTG 4 cut(s) 376, 2916, 3109, 3313
Tth111I GACNNNGTC 1 cut(s) 826
VneI GTGCAC 2 cut(s) 1098, 1754
VpaK11BI GGWCC 1 cut(s) 2072
VspI ATTAAT 1 cut(s) 914
XagI CCTNNNNNAGG 1 cut(s) 1199
XapI RAATTY 6 cut(s) 26, 142, 418, 466, 535, 1711
XbaI TCTAGA 1 cut(s) 3283
XceI RCATGY 1 cut(s) 1192
XhoI CTCGAG 1 cut(s) 42
XmaI CCCGGG 1 cut(s) 2458
XmnI GAANNNNTTC 1 cut(s) 1408
XspI CTAG 6 cut(s) 411, 2013, 2382, 3095, 3284, 3317
ZrmI AGTACT 1 cut(s) 2563
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.