Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001148

Physical Location & Seq

Genomic Coordinates Reverse (-)

31534355 .. 31538124

Gene Structure 3770 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1236500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1215 bp

ATGGAGGAGAGAGTGGGAGAGGCAGAACCAGAACAGCAGCAAGAGGAGGAGCAGGTAGTGAATCCATGGACGGTGTTGGCCAAGGGTGGCGGGAAAATCGACTACGACAAGCTCATCGACCAGTTCGGCTGCCAGAGAATCGACCAGCAGCTCGTGGATCGCATCCACTGCCTCACCGGCCGTCCTCCCCACGTCTTCCTCCGCCGTGGCGTCTTCTTTGCCCACAGGGACTTGAATGAGATTCTGGATGCCTACGAGAGAGGAGACAAGTTCTATTTGTACACAGGAAGAGGACCTTCCTCCGAAGCTTTGCATTTGGGGCATCTTATCCCCTTCATGTTCACTAAATATTTGCAAGATGTCTTCAAGGTTCCTCTCGTTGTACAACTTACCGATGATGAGAAGTGCATGTGGAAAAATCTGACTGTGGAAGAGAGCCAGAGACTTGCTAGGGAGAATGCTAAAGACATTATTGCTTGTGGTTTTGACATATCAAAAACCTTCATCTTCTCCGATTTTGATTTCGTTGGTGGTGCCTTCTACAAGAACATGGTGAAGGTTGGAAAGTGTGTCACATATAATAAGGTTGTTGGTATCTTTGGTTTCACTGGAGAAGATCACATAGGAAAAGTTAGTTTTCCACCTGTACAGGCAGTTCCCTCATTTCCAAGTTCATTTCCTCATCTCTTCTCTGGCCAAGACAATCTTCGATGCTTAATACCTTGTGCGATTGACCAGGATCCTTATTTTAGAATGACACGAGATGTTGCTCCACGAATTGGATATCACAAGCCTGCATTGATTGAGTCTTCTTTCTTCCCTGCCCTGCAGGGAGAGACTGGAAAAATGTCGGCTAGTGATCCAAATTCGGCCATATATGTAACTGATGCTGCAAAGGAAATTAAGAACAAGATAAACAGGTATGCATTTTCTGGTGGACAAGATTCGGTAGAGAAACATAGAGAGCTTGGAGCAAATCTTGAGGTAGATATTCCATTCAAATATCTGAGCTTTTTCCTAGACGACGATGATAAACTCGAAGAGATACGGAAGGAGTATGGTTCCGGACGCATGCTAACAGGTGAAGTGAAGCAACTTCTTGTTCAAGTTTTAACCGAAATGGTAGAAAGACATCGTAGGGCTAGGGCTGCTGTGACTGATGAGATGGTGGACGCATTTATGGCAGTGAGGCCTCTACCGAATATGTTCAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004830 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006436 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_1b

Protein Analysis

405

Amino Acids

45.93

Weight (kDa)

5.58

Isoelectric Point (pI)

42.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1b	PF00579	89 - 374	4.4e-20	tRNA synthetases class I (W and Y)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04600 AT3G04600 AT3G04600
fragaria_vesca	FvH4_4g11950 FvH4_4g11950 FvH4_4g11950 FvH4_4g11950 FvH4_5g27321
malus_domestica	MD04G1236500.v1.1 MD12G1255800.v1.1
prunus_persica	Prupe.7G057000_v2.0.a1 Prupe.7G057800_v2.0.a1
pyrus_communis	pycom04g21040 pycom12g23440
rosa_chinensis	RchiOBHm_Chr3g0493481 RchiOBHm_Chr7g0219201 RchiOBHm_Chr7g0219221 RchiOBHm_Chr7g0223011
rosa_laevigata	RLG00000002003 RLG00000002302 RLG00000002303 RLG00000022713
rosa_multiflora	Rmu_sc0000868.1_g000014 Rmu_sc0006373.1_g000002 Rmu_sc0006514.1_g000024 Rmu_sc0007422.1_g000005 Rmu_sc0009785.1_g000024 Rmu_sc0039697.1_g000001
rosa_roxburghii	Rroxscaffold_3G00234440 Rroxscaffold_3G00236350 Rroxscaffold_3G00237890 Rroxscaffold_3G00237970 Rroxscaffold_3G00238030 Rroxscaffold_3G00238960 Rroxscaffold_3G00238970 Rroxscaffold_5G00377610 Rroxscaffold_6G00391570 Rroxscaffold_7G00199590 Rroxscaffold_7G00199600
rosa_rugosa	Rorug07G0177500 Rorug07G0177500 Rorug07G0212800
rosa_samantha	Rh3AG310200 Rh3BG346300 Rh3CG344200 Rh3DG345400 Rh7AG319000 Rh7AG326700 Rh7AG357900 Rh7BG310900 Rh7BG346100 Rh7CG376000 Rh7DG318100 Rh7DG318200 Rh7DG352800
rosa_wichuraiana	Rw3G027260 Rw7G027160 Rw7G030440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	43
AccB1I	GGYRCC	1 cut(s)	533
AccB7I	CCANNNNNTGG	1 cut(s)	779
AccIII	TCCGGA	1 cut(s)	1064
AciI	CCGC	2 cut(s)	90, 202
AclWI	GGATC	4 cut(s)	165, 734, 747, 854
AcoI	YGGCCR	4 cut(s)	78, 178, 694, 870
AcsI	RAATTY	1 cut(s)	865
AcyI	GRCGYC	1 cut(s)	210
AfaI	GTAC	3 cut(s)	281, 384, 648
AfiI	CCNNNNNNNGG	1 cut(s)	779
AgsI	TTSAA	4 cut(s)	235, 367, 1000, 1106
AjiI	CACGTC	1 cut(s)	193
AjnI	CCWGG	1 cut(s)	735
AjuI	GAANNNNNNNTTGG	2 cut(s)	690, 722
AluBI	AGCT	6 cut(s)	112, 151, 308, 967, 1011, 1212
AluI	AGCT	6 cut(s)	112, 151, 308, 967, 1011, 1212
Alw26I	GTCTC	3 cut(s)	258, 436, 830
AlwI	GGATC	4 cut(s)	165, 734, 747, 854
Aor13HI	TCCGGA	1 cut(s)	1064
AoxI	GGCC	5 cut(s)	78, 178, 694, 870, 1190
ApeKI	GCWGC	5 cut(s)	37, 129, 148, 890, 1148
ApoI	RAATTY	1 cut(s)	865
Asp700I	GAANNNNTTC	1 cut(s)	1205
AspS9I	GGNCC	1 cut(s)	293
AsuHPI	GGTGA	3 cut(s)	166, 565, 1094
AvaII	GGWCC	1 cut(s)	293
BalI	TGGCCA	2 cut(s)	80, 696
BamHI	GGATCC	1 cut(s)	739
BanI	GGYRCC	1 cut(s)	533
BauI	CACGAG	2 cut(s)	152, 759
BbsI	GAAGAC	4 cut(s)	187, 205, 355, 801
BbvI	GCAGC	5 cut(s)	49, 116, 160, 877, 1135
BccI	CCATC	1 cut(s)	1159
BceAI	ACGGC	2 cut(s)	165, 189
BciT130I	CCWGG	1 cut(s)	737
BcoDI	GTCTC	3 cut(s)	258, 436, 830
BfaI	CTAG	4 cut(s)	450, 855, 1019, 1143
BfmI	CTRYAG	1 cut(s)	827
BfuAI	ACCTGC	1 cut(s)	43
BglI	GCCNNNNNGGC	1 cut(s)	177
BisI	GCNGC	5 cut(s)	38, 130, 149, 891, 1149
BlsI	GCNGC	5 cut(s)	39, 131, 150, 892, 1150
Bme1390I	CCNGG	1 cut(s)	737
Bme18I	GGWCC	1 cut(s)	293
BmgBI	CACGTC	1 cut(s)	193
BmgT120I	GGNCC	1 cut(s)	293
BmiI	GGNNCC	4 cut(s)	372, 535, 741, 1063
BmrFI	CCNGG	1 cut(s)	737
BmsI	GCATC	5 cut(s)	171, 238, 331, 701, 877
BpiI	GAAGAC	4 cut(s)	187, 205, 355, 801
BpmI	CTGGAG	1 cut(s)	630
BpuEI	CTTGAG	1 cut(s)	1001
BsaHI	GRCGYC	1 cut(s)	210
BsaJI	CCNNGG	3 cut(s)	65, 81, 205
BsaWI	WCCGGW	1 cut(s)	1064
Bsc4I	CCNNNNNNNGG	1 cut(s)	779
Bse118I	RCCGGY	1 cut(s)	176
Bse1I	ACTGG	3 cut(s)	121, 613, 844
BseAI	TCCGGA	1 cut(s)	1064
BseBI	CCWGG	1 cut(s)	737
BseDI	CCNNGG	3 cut(s)	65, 81, 205
BseGI	GGATG	2 cut(s)	162, 253
BseLI	CCNNNNNNNGG	1 cut(s)	779
BseMII	CTCAG	1 cut(s)	998
BseNI	ACTGG	3 cut(s)	121, 613, 844
BseRI	GAGGAG	4 cut(s)	20, 59, 62, 276
BseX3I	CGGCCG	1 cut(s)	178
BseXI	GCAGC	5 cut(s)	49, 116, 160, 877, 1135
Bsh1285I	CGRYCG	1 cut(s)	181
BshFI	GGCC	5 cut(s)	80, 180, 696, 872, 1192
BshNI	GGYRCC	1 cut(s)	533
BsiEI	CGRYCG	1 cut(s)	181
BsiSI	CCGG	2 cut(s)	177, 1065
BslFI	GGGAC	1 cut(s)	242
BslI	CCNNNNNNNGG	1 cut(s)	779
BsmAI	GTCTC	3 cut(s)	258, 436, 830
BsmFI	GGGAC	1 cut(s)	242
BsmI	GAATGC	1 cut(s)	463
BsnI	GGCC	5 cut(s)	80, 180, 696, 872, 1192
Bsp13I	TCCGGA	1 cut(s)	1064
Bsp1407I	TGTACA	3 cut(s)	279, 382, 646
Bsp143I	GATC	4 cut(s)	157, 616, 739, 859
Bsp19I	CCATGG	1 cut(s)	65
BspACI	CCGC	2 cut(s)	90, 202
BspANI	GGCC	5 cut(s)	80, 180, 696, 872, 1192
BspCNI	CTCAG	1 cut(s)	999
BspEI	TCCGGA	1 cut(s)	1064
BspLI	GGNNCC	4 cut(s)	372, 535, 741, 1063
BspMAI	CTGCAG	1 cut(s)	831
BspMI	ACCTGC	1 cut(s)	43
BspPI	GGATC	4 cut(s)	165, 734, 747, 854
BspT107I	GGYRCC	1 cut(s)	533
BsrFI	RCCGGY	1 cut(s)	176
BsrGI	TGTACA	3 cut(s)	279, 382, 646
BsrI	ACTGG	3 cut(s)	121, 613, 844
BssAI	RCCGGY	1 cut(s)	176
BssECI	CCNNGG	3 cut(s)	65, 81, 205
BssMI	GATC	4 cut(s)	157, 616, 739, 859
BssNI	GRCGYC	1 cut(s)	210
BssSI	CACGAG	2 cut(s)	152, 759
BssT1I	CCWWGG	2 cut(s)	65, 81
Bst2BI	CACGAG	2 cut(s)	152, 759
Bst2UI	CCWGG	1 cut(s)	737
Bst4CI	ACNGT	2 cut(s)	73, 427
Bst6I	CTCTTC	4 cut(s)	283, 426, 692, 1035
BstACI	GRCGYC	1 cut(s)	210
BstAPI	GCANNNNNTGC	1 cut(s)	168
BstAUI	TGTACA	3 cut(s)	279, 382, 646
BstC8I	GCNNGC	2 cut(s)	795, 1073
BstDEI	CTNAG	1 cut(s)	1007
BstDSI	CCRYGG	2 cut(s)	65, 205
BstF5I	GGATG	2 cut(s)	162, 253
BstKTI	GATC	4 cut(s)	160, 619, 742, 862
BstMAI	GTCTC	3 cut(s)	258, 436, 830
BstMBI	GATC	4 cut(s)	157, 616, 739, 859
BstMCI	CGRYCG	1 cut(s)	181
BstMWI	GCNNNNNNNGC	5 cut(s)	168, 177, 319, 1148, 1181
BstNI	CCWGG	1 cut(s)	737
BstNSI	RCATGY	2 cut(s)	412, 1075
BstSCI	CCNGG	1 cut(s)	735
BstSFI	CTRYAG	1 cut(s)	827
BstV1I	GCAGC	5 cut(s)	49, 116, 160, 877, 1135
BstV2I	GAAGAC	4 cut(s)	187, 205, 355, 801
BstX2I	RGATCY	1 cut(s)	739
BstYI	RGATCY	1 cut(s)	739
BstZI	CGGCCG	1 cut(s)	178
BsuRI	GGCC	5 cut(s)	80, 180, 696, 872, 1192
BtgI	CCRYGG	2 cut(s)	65, 205
BtrI	CACGTC	1 cut(s)	193
BtsCI	GGATG	2 cut(s)	162, 253
BtsI	GCAGTG	2 cut(s)	166, 1191
BtsIMutI	CAGTG	3 cut(s)	166, 606, 1191
BveI	ACCTGC	1 cut(s)	43
Cac8I	GCNNGC	2 cut(s)	795, 1073
Cfr10I	RCCGGY	1 cut(s)	176
Cfr13I	GGNCC	1 cut(s)	293
CseI	GACGC	3 cut(s)	199, 1077, 1181
Csp6I	GTAC	3 cut(s)	280, 383, 647
CviAII	CATG	5 cut(s)	66, 337, 409, 550, 1072
CviQI	GTAC	3 cut(s)	280, 383, 647
DdeI	CTNAG	1 cut(s)	1007
DpnI	GATC	4 cut(s)	159, 618, 741, 861
DpnII	GATC	4 cut(s)	157, 616, 739, 859
EaeI	YGGCCR	4 cut(s)	78, 178, 694, 870
EagI	CGGCCG	1 cut(s)	178
Eam1104I	CTCTTC	4 cut(s)	283, 426, 692, 1035
EarI	CTCTTC	4 cut(s)	283, 426, 692, 1035
EciI	GGCGGA	1 cut(s)	191
EclXI	CGGCCG	1 cut(s)	178
Eco130I	CCWWGG	2 cut(s)	65, 81
Eco147I	AGGCCT	1 cut(s)	1192
Eco32I	GATATC	1 cut(s)	785
Eco47I	GGWCC	1 cut(s)	293
Eco52I	CGGCCG	1 cut(s)	178
EcoO109I	RGGNCCY	1 cut(s)	293
EcoRII	CCWGG	1 cut(s)	735
EcoRV	GATATC	1 cut(s)	785
EcoT14I	CCWWGG	2 cut(s)	65, 81
EcoT22I	ATGCAT	1 cut(s)	928
ErhI	CCWWGG	2 cut(s)	65, 81
FaeI	CATG	5 cut(s)	69, 340, 412, 553, 1075
FalI	AAGNNNNNCTT	4 cut(s)	280, 312, 690, 722
FaqI	GGGAC	1 cut(s)	242
FatI	CATG	5 cut(s)	65, 336, 408, 549, 1071
FauI	CCCGC	1 cut(s)	83
Fnu4HI	GCNGC	5 cut(s)	38, 130, 149, 891, 1149
FokI	GGATG	2 cut(s)	149, 260
Fsp4HI	GCNGC	5 cut(s)	38, 130, 149, 891, 1149
FspBI	CTAG	4 cut(s)	450, 855, 1019, 1143
GluI	GCNGC	5 cut(s)	38, 130, 149, 891, 1149
GsuI	CTGGAG	1 cut(s)	630
HaeIII	GGCC	5 cut(s)	80, 180, 696, 872, 1192
HapII	CCGG	2 cut(s)	177, 1065
HgaI	GACGC	3 cut(s)	199, 1077, 1181
Hin1I	GRCGYC	1 cut(s)	210
Hin1II	CATG	5 cut(s)	69, 340, 412, 553, 1075
HindIII	AAGCTT	1 cut(s)	306
HinfI	GANTC	5 cut(s)	61, 138, 241, 806, 944
HpaII	CCGG	2 cut(s)	177, 1065
HphI	GGTGA	3 cut(s)	166, 565, 1094
Hpy166II	GTNNAC	4 cut(s)	282, 342, 938, 1171
Hpy188I	TCNGA	4 cut(s)	304, 423, 514, 1008
Hpy188III	TCNNGA	3 cut(s)	245, 980, 1065
Hpy8I	GTNNAC	4 cut(s)	282, 342, 938, 1171
Hpy99I	CGWCG	1 cut(s)	1028
HpyAV	CCTTC	6 cut(s)	306, 343, 511, 547, 550, 1045
HpyCH4III	ACNGT	2 cut(s)	73, 427
HpyCH4IV	ACGT	1 cut(s)	192
HpyCH4V	TGCA	7 cut(s)	313, 355, 408, 797, 829, 893, 926
HpyF10VI	GCNNNNNNNGC	5 cut(s)	168, 177, 319, 1148, 1181
HpyF3I	CTNAG	1 cut(s)	1007
HpySE526I	ACGT	1 cut(s)	192
Hsp92I	GRCGYC	1 cut(s)	210
Hsp92II	CATG	5 cut(s)	69, 340, 412, 553, 1075
Kpn2I	TCCGGA	1 cut(s)	1064
Kzo9I	GATC	4 cut(s)	157, 616, 739, 859
LmnI	GCTCC	3 cut(s)	49, 775, 971
Lsp1109I	GCAGC	5 cut(s)	49, 116, 160, 877, 1135
LweI	GCATC	5 cut(s)	171, 238, 331, 701, 877
MaeI	CTAG	4 cut(s)	450, 855, 1019, 1143
MaeII	ACGT	1 cut(s)	192
MaeIII	GTNAC	3 cut(s)	571, 880, 1153
MalI	GATC	4 cut(s)	159, 618, 741, 861
MboI	GATC	4 cut(s)	157, 616, 739, 859
MflI	RGATCY	1 cut(s)	739
MlsI	TGGCCA	2 cut(s)	80, 696
MluCI	AATT	3 cut(s)	777, 865, 900
MluNI	TGGCCA	2 cut(s)	80, 696
MlyI	GAGTC	1 cut(s)	815
MmeI	TCCRAC	1 cut(s)	541
Mox20I	TGGCCA	2 cut(s)	80, 696
Mph1103I	ATGCAT	1 cut(s)	928
MroI	TCCGGA	1 cut(s)	1064
MroXI	GAANNNNTTC	1 cut(s)	1205
MscI	TGGCCA	2 cut(s)	80, 696
MseI	TTAA	3 cut(s)	716, 903, 1112
Msp20I	TGGCCA	2 cut(s)	80, 696
MspI	CCGG	2 cut(s)	177, 1065
MspR9I	CCNGG	1 cut(s)	737
Mva1269I	GAATGC	1 cut(s)	463
MvaI	CCWGG	1 cut(s)	737
MwoI	GCNNNNNNNGC	5 cut(s)	168, 177, 319, 1148, 1181
NcoI	CCATGG	1 cut(s)	65
NdeII	GATC	4 cut(s)	157, 616, 739, 859
NlaIII	CATG	5 cut(s)	69, 340, 412, 553, 1075
NlaIV	GGNNCC	4 cut(s)	372, 535, 741, 1063
NmuCI	GTSAC	2 cut(s)	571, 1153
NsiI	ATGCAT	1 cut(s)	928
NspI	RCATGY	2 cut(s)	412, 1075
PaeI	GCATGC	1 cut(s)	1075
PceI	AGGCCT	1 cut(s)	1192
PctI	GAATGC	1 cut(s)	463
PdmI	GAANNNNTTC	1 cut(s)	1205
PfeI	GAWTC	4 cut(s)	61, 138, 241, 944
PflMI	CCANNNNNTGG	1 cut(s)	779
PkrI	GCNGC	5 cut(s)	39, 131, 150, 892, 1150
PleI	GAGTC	1 cut(s)	814
PpsI	GAGTC	1 cut(s)	814
PpuMI	RGGWCCY	1 cut(s)	293
Psp5II	RGGWCCY	1 cut(s)	293
Psp6I	CCWGG	1 cut(s)	735
PspGI	CCWGG	1 cut(s)	735
PspN4I	GGNNCC	4 cut(s)	372, 535, 741, 1063
PspPI	GGNCC	1 cut(s)	293
PspPPI	RGGWCCY	1 cut(s)	293
PsrI	GAACNNNNNNTAC	2 cut(s)	639, 671
PstI	CTGCAG	1 cut(s)	831
PsuI	RGATCY	1 cut(s)	739
RsaI	GTAC	3 cut(s)	281, 384, 648
RsaNI	GTAC	3 cut(s)	280, 383, 647
SaqAI	TTAA	3 cut(s)	716, 903, 1112
SatI	GCNGC	5 cut(s)	38, 130, 149, 891, 1149
Sau3AI	GATC	4 cut(s)	157, 616, 739, 859
Sau96I	GGNCC	1 cut(s)	293
SbfI	CCTGCAGG	1 cut(s)	831
SchI	GAGTC	1 cut(s)	815
ScrFI	CCNGG	1 cut(s)	737
SdaI	CCTGCAGG	1 cut(s)	831
SfaNI	GCATC	5 cut(s)	171, 238, 331, 701, 877
SfcI	CTRYAG	1 cut(s)	827
SinI	GGWCC	1 cut(s)	293
SmlI	CTYRAG	1 cut(s)	980
SmoI	CTYRAG	1 cut(s)	980
SphI	GCATGC	1 cut(s)	1075
Sse8387I	CCTGCAGG	1 cut(s)	831
Sse9I	AATT	3 cut(s)	777, 865, 900
SseBI	AGGCCT	1 cut(s)	1192
SsiI	CCGC	2 cut(s)	90, 202
SspI	AATATT	1 cut(s)	350
SspMI	CTAG	4 cut(s)	450, 855, 1019, 1143
StuI	AGGCCT	1 cut(s)	1192
StyD4I	CCNGG	1 cut(s)	735
StyI	CCWWGG	2 cut(s)	65, 81
TaaI	ACNGT	2 cut(s)	73, 427
TaiI	ACGT	1 cut(s)	195
TaqI	TCGA	5 cut(s)	99, 117, 141, 709, 1038
TasI	AATT	3 cut(s)	777, 865, 900
TatI	WGTACW	3 cut(s)	279, 382, 646
TfiI	GAWTC	4 cut(s)	61, 138, 241, 944
Tru1I	TTAA	3 cut(s)	716, 903, 1112
Tru9I	TTAA	3 cut(s)	716, 903, 1112
TscAI	CASTG	3 cut(s)	173, 613, 1191
TseFI	GTSAC	2 cut(s)	571, 1153
TseI	GCWGC	5 cut(s)	37, 129, 148, 890, 1148
Tsp45I	GTSAC	2 cut(s)	571, 1153
TspDTI	ATGAA	3 cut(s)	325, 493, 663
TspGWI	ACGGA	1 cut(s)	1063
TspRI	CASTG	3 cut(s)	173, 613, 1191
Van91I	CCANNNNNTGG	1 cut(s)	779
VpaK11BI	GGWCC	1 cut(s)	293
XapI	RAATTY	1 cut(s)	865
XceI	RCATGY	2 cut(s)	412, 1075
XmnI	GAANNNNTTC	1 cut(s)	1205
XspI	CTAG	4 cut(s)	450, 855, 1019, 1143
Zsp2I	ATGCAT	1 cut(s)	928

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.