Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001148

Physical Location & Seq

Genomic Coordinates Reverse (-)

32358913 .. 32362658

Gene Structure 3746 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1255800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGAAAACACCAAAATACCCTCCGAAGAAAAAGAAAGGAAGAAGACGAAGACGCCGCCGATTTTTACAGACAGTGAGCGAACGACGGAGAGAAAGAGAGAGAGAGAGAGTGACGATGGAGAAAAGAGAGGGAGAGGCAGAACCAGAACAGAAGGAAGAGGAGGAGCAGGTAGTGAATCCATGGATGGTGTCGGCCAAAGGTGGCGGGAAAATCGACTACGACAAGCTCATTGACCAGTTCGGCTGCCAGAGAATCGACCAGTCGCTCGTCGACCGCATTCACCGCCTCACCGGCCGTCCTCCCCACGTCTTCCTCCGCCGCGGCGTCTTCTTTGCCCACAGGGACTTGAATGAGATTCTGGATGCCTACGAGAGGGGAGACAAGTTCTATTTGTACACGGGACGAGGGCCCTCCTCCGAGGCTCTGCATTTGGGGCATCTTATCCCCTTCATGTTCACTAAATATTTGCAAGAGGTCTTCAAGGTTCCGCTCGTTGTACAACTTACCGACGATGAGAAGTGCATGTGGAAAAATCTGACCGTGGAAGAGAGCCAGAGACTTGCCAGGGAGAATGCTAAAGACATTATTGCTTGTGGTTTTGACATATCAAAAACCTTCATCTTCTCCGATTTCGACTTCGTTGGTGGTGCCTTCTACAAGAATATGGTGAAAGTTGGAAAGTGTGTCACATATAATAAGGTTGTTGGTATCTTTGGTTTCACTGGAGAAGATCACATAGGAAAAGTTAGTTTTCCACCTGTGCAGGCAGTTCCATCATTTCCAAGTTCATTTCCACATCTCTTCCCTGGCCAAGACAATCTTCGTTGCTTAATACCTTGCGCGATTGACCAGGATCCTTATTTTAGAATGACACGAGATGTTGCTCCACGAATTGGGTATCACAAACCTGCATTGATTGAGTCTTCTTTCTTCCCTGCCCTGCAGGGAGAGACTGGAAAAATGTCGGCTAGTGATCCAAATTCTGCCATATATGTAACTGATTCTGCAAAGGAAATTAAGAACAAGATCAACAGGTATGCATTTTCTGGTGGACAAGATTCAGTAGAGAAACATAGAGAGCTTGGAGCAAATCTCGAGGTAGATATTCCATTCAAATATCTAAGCTTTTTCCTAGACGATGATGATAAACTCGAAGAGATACGGAAGGAGTATGGTTCCGGGCGCATGCTAACAGGTGAAGTGAAGCAACTGCTTGTTGAAGTTTTGACGGAAATGGTAGAAAGACATCGTAGGGCTAGGGCTGTTGTGACTGATGAGATGGTGGACGCATTCATGGCGGTGAGGCCCCTACCCAATATGTTCATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004830 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006436 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_1b

Protein Analysis

443

Amino Acids

51.0

Weight (kDa)

8.79

Isoelectric Point (pI)

53.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1b	PF00579	127 - 412	2.1e-20	tRNA synthetases class I (W and Y)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04600 AT3G04600 AT3G04600
fragaria_vesca	FvH4_4g11950 FvH4_4g11950 FvH4_4g11950 FvH4_4g11950 FvH4_5g27321
malus_domestica	MD04G1236500.v1.1 MD12G1255800.v1.1
prunus_persica	Prupe.7G057000_v2.0.a1 Prupe.7G057800_v2.0.a1
pyrus_communis	pycom04g21040 pycom12g23440
rosa_chinensis	RchiOBHm_Chr3g0493481 RchiOBHm_Chr7g0219201 RchiOBHm_Chr7g0219221 RchiOBHm_Chr7g0223011
rosa_laevigata	RLG00000002003 RLG00000002302 RLG00000002303 RLG00000022713
rosa_multiflora	Rmu_sc0000868.1_g000014 Rmu_sc0006373.1_g000002 Rmu_sc0006514.1_g000024 Rmu_sc0007422.1_g000005 Rmu_sc0009785.1_g000024 Rmu_sc0039697.1_g000001
rosa_roxburghii	Rroxscaffold_3G00234440 Rroxscaffold_3G00236350 Rroxscaffold_3G00237890 Rroxscaffold_3G00237970 Rroxscaffold_3G00238030 Rroxscaffold_3G00238960 Rroxscaffold_3G00238970 Rroxscaffold_5G00377610 Rroxscaffold_6G00391570 Rroxscaffold_7G00199590 Rroxscaffold_7G00199600
rosa_rugosa	Rorug07G0177500 Rorug07G0177500 Rorug07G0212800
rosa_samantha	Rh3AG310200 Rh3BG346300 Rh3CG344200 Rh3DG345400 Rh7AG319000 Rh7AG326700 Rh7AG357900 Rh7BG310900 Rh7BG346100 Rh7CG376000 Rh7DG318100 Rh7DG318200 Rh7DG352800
rosa_wichuraiana	Rw3G027260 Rw7G027160 Rw7G030440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	157, 916
AccB1I	GGYRCC	1 cut(s)	647
AccB7I	CCANNNNNTGG	1 cut(s)	893
AccBSI	CCGCTC	1 cut(s)	490
AccI	GTMKAC	1 cut(s)	270
AccII	CGCG	2 cut(s)	321, 842
AciI	CCGC	9 cut(s)	55, 204, 274, 283, 316, 319, 321, 488, 1298
AclWI	GGATC	3 cut(s)	848, 861, 968
AcoI	YGGCCR	3 cut(s)	192, 292, 808
AcsI	RAATTY	1 cut(s)	979
AcyI	GRCGYC	2 cut(s)	52, 324
AfaI	GTAC	2 cut(s)	395, 498
AfiI	CCNNNNNNNGG	2 cut(s)	372, 893
AgsI	TTSAA	4 cut(s)	349, 481, 1114, 1220
AjiI	CACGTC	1 cut(s)	307
AjnI	CCWGG	3 cut(s)	563, 805, 849
AjuI	GAANNNNNNNTTGG	2 cut(s)	804, 836
AluBI	AGCT	3 cut(s)	226, 1081, 1125
AluI	AGCT	3 cut(s)	226, 1081, 1125
Alw26I	GTCTC	3 cut(s)	372, 550, 944
AlwI	GGATC	3 cut(s)	848, 861, 968
Ama87I	CYCGRG	1 cut(s)	1094
AoxI	GGCC	5 cut(s)	192, 292, 407, 808, 1304
ApaI	GGGCCC	1 cut(s)	411
ApeKI	GCWGC	1 cut(s)	243
ApoI	RAATTY	1 cut(s)	979
AspLEI	GCGC	2 cut(s)	842, 1185
AspS9I	GGNCC	3 cut(s)	407, 408, 1305
AsuC2I	CCSGG	1 cut(s)	1180
AsuHPI	GGTGA	5 cut(s)	272, 280, 679, 1208, 1312
AvaI	CYCGRG	1 cut(s)	1094
BaeGI	GKGCMC	1 cut(s)	411
BalI	TGGCCA	1 cut(s)	810
BamHI	GGATCC	1 cut(s)	853
BanI	GGYRCC	1 cut(s)	647
BanII	GRGCYC	1 cut(s)	411
BauI	CACGAG	1 cut(s)	873
BbsI	GAAGAC	6 cut(s)	49, 55, 301, 319, 469, 915
BbvI	GCAGC	1 cut(s)	230
BccI	CCATC	4 cut(s)	109, 178, 781, 1273
BceAI	ACGGC	1 cut(s)	279
BciT130I	CCWGG	3 cut(s)	565, 807, 851
BcnI	CCSGG	1 cut(s)	1180
BcoDI	GTCTC	3 cut(s)	372, 550, 944
BfaI	CTAG	3 cut(s)	969, 1133, 1257
BfmI	CTRYAG	1 cut(s)	941
BfuAI	ACCTGC	2 cut(s)	157, 916
BglI	GCCNNNNNGGC	1 cut(s)	291
BisI	GCNGC	4 cut(s)	55, 244, 319, 322
BlsI	GCNGC	4 cut(s)	56, 245, 320, 323
Bme1390I	CCNGG	4 cut(s)	565, 807, 851, 1180
BmeT110I	CYCGRG	1 cut(s)	1094
BmgBI	CACGTC	1 cut(s)	307
BmgT120I	GGNCC	3 cut(s)	407, 408, 1305
BmiI	GGNNCC	6 cut(s)	409, 486, 649, 855, 1177, 1307
BmrFI	CCNGG	4 cut(s)	565, 807, 851, 1180
BmsI	GCATC	2 cut(s)	352, 445
BpiI	GAAGAC	6 cut(s)	49, 55, 301, 319, 469, 915
BplI	GAGNNNNNCTC	2 cut(s)	1077, 1109
BpmI	CTGGAG	1 cut(s)	744
BpuMI	CCSGG	1 cut(s)	1180
BsaHI	GRCGYC	2 cut(s)	52, 324
BsaJI	CCNNGG	6 cut(s)	179, 319, 417, 540, 564, 805
Bsc4I	CCNNNNNNNGG	2 cut(s)	372, 893
Bse118I	RCCGGY	1 cut(s)	290
Bse1I	ACTGG	4 cut(s)	235, 259, 727, 958
BseBI	CCWGG	3 cut(s)	565, 807, 851
BseDI	CCNNGG	6 cut(s)	179, 319, 417, 540, 564, 805
BseGI	GGATG	2 cut(s)	189, 367
BseLI	CCNNNNNNNGG	2 cut(s)	372, 893
BseNI	ACTGG	4 cut(s)	235, 259, 727, 958
BseRI	GAGGAG	3 cut(s)	173, 176, 403
BseSI	GKGCMC	1 cut(s)	411
BseX3I	CGGCCG	1 cut(s)	292
BseXI	GCAGC	1 cut(s)	230
BsgI	GTGCAG	1 cut(s)	782
Bsh1236I	CGCG	2 cut(s)	321, 842
Bsh1285I	CGRYCG	2 cut(s)	274, 295
BshFI	GGCC	5 cut(s)	194, 294, 409, 810, 1306
BshNI	GGYRCC	1 cut(s)	647
BsiEI	CGRYCG	2 cut(s)	274, 295
BsiHKCI	CYCGRG	1 cut(s)	1094
BsiSI	CCGG	2 cut(s)	291, 1179
BslFI	GGGAC	2 cut(s)	356, 414
BslI	CCNNNNNNNGG	2 cut(s)	372, 893
BsmAI	GTCTC	3 cut(s)	372, 550, 944
BsmFI	GGGAC	2 cut(s)	356, 414
BsmI	GAATGC	3 cut(s)	276, 577, 1289
BsnI	GGCC	5 cut(s)	194, 294, 409, 810, 1306
BsoBI	CYCGRG	1 cut(s)	1094
Bsp120I	GGGCCC	1 cut(s)	407
Bsp1286I	GDGCHC	1 cut(s)	411
Bsp1407I	TGTACA	2 cut(s)	393, 496
Bsp143I	GATC	4 cut(s)	730, 853, 973, 1026
Bsp19I	CCATGG	1 cut(s)	179
BspACI	CCGC	9 cut(s)	55, 204, 274, 283, 316, 319, 321, 488, 1298
BspANI	GGCC	5 cut(s)	194, 294, 409, 810, 1306
BspFNI	CGCG	2 cut(s)	321, 842
BspLI	GGNNCC	6 cut(s)	409, 486, 649, 855, 1177, 1307
BspMAI	CTGCAG	1 cut(s)	945
BspMI	ACCTGC	2 cut(s)	157, 916
BspPI	GGATC	3 cut(s)	848, 861, 968
BspT107I	GGYRCC	1 cut(s)	647
BsrBI	CCGCTC	1 cut(s)	490
BsrFI	RCCGGY	1 cut(s)	290
BsrGI	TGTACA	2 cut(s)	393, 496
BsrI	ACTGG	4 cut(s)	235, 259, 727, 958
BssAI	RCCGGY	1 cut(s)	290
BssECI	CCNNGG	6 cut(s)	179, 319, 417, 540, 564, 805
BssMI	GATC	4 cut(s)	730, 853, 973, 1026
BssNI	GRCGYC	2 cut(s)	52, 324
BssSI	CACGAG	1 cut(s)	873
BssT1I	CCWWGG	1 cut(s)	179
Bst2BI	CACGAG	1 cut(s)	873
Bst2UI	CCWGG	3 cut(s)	565, 807, 851
Bst4CI	ACNGT	2 cut(s)	73, 541
Bst6I	CTCTTC	4 cut(s)	150, 540, 806, 1149
BstACI	GRCGYC	2 cut(s)	52, 324
BstAUI	TGTACA	2 cut(s)	393, 496
BstC8I	GCNNGC	2 cut(s)	765, 1187
BstDEI	CTNAG	1 cut(s)	1121
BstDSI	CCRYGG	3 cut(s)	179, 319, 540
BstENI	CCTNNNNNAGG	1 cut(s)	370
BstF5I	GGATG	2 cut(s)	189, 367
BstFNI	CGCG	2 cut(s)	321, 842
BstHHI	GCGC	2 cut(s)	842, 1185
BstKTI	GATC	4 cut(s)	733, 856, 976, 1029
BstMAI	GTCTC	3 cut(s)	372, 550, 944
BstMBI	GATC	4 cut(s)	730, 853, 973, 1026
BstMCI	CGRYCG	2 cut(s)	274, 295
BstMWI	GCNNNNNNNGC	4 cut(s)	282, 291, 433, 1295
BstNI	CCWGG	3 cut(s)	565, 807, 851
BstNSI	RCATGY	2 cut(s)	526, 1189
BstSCI	CCNGG	4 cut(s)	563, 805, 849, 1178
BstSFI	CTRYAG	1 cut(s)	941
BstSLI	GKGCMC	1 cut(s)	411
BstUI	CGCG	2 cut(s)	321, 842
BstV1I	GCAGC	1 cut(s)	230
BstV2I	GAAGAC	6 cut(s)	49, 55, 301, 319, 469, 915
BstX2I	RGATCY	1 cut(s)	853
BstYI	RGATCY	1 cut(s)	853
BstZI	CGGCCG	1 cut(s)	292
BsuRI	GGCC	5 cut(s)	194, 294, 409, 810, 1306
BtgI	CCRYGG	3 cut(s)	179, 319, 540
BtrI	CACGTC	1 cut(s)	307
BtsCI	GGATG	2 cut(s)	189, 367
BtsIMutI	CAGTG	2 cut(s)	78, 720
BveI	ACCTGC	2 cut(s)	157, 916
Cac8I	GCNNGC	2 cut(s)	765, 1187
CfoI	GCGC	2 cut(s)	842, 1185
Cfr10I	RCCGGY	1 cut(s)	290
Cfr13I	GGNCC	3 cut(s)	407, 408, 1305
Cfr42I	CCGCGG	1 cut(s)	322
CseI	GACGC	3 cut(s)	60, 313, 1295
Csp6I	GTAC	2 cut(s)	394, 497
CviAII	CATG	5 cut(s)	180, 451, 523, 1186, 1294
CviQI	GTAC	2 cut(s)	394, 497
DdeI	CTNAG	1 cut(s)	1121
DpnI	GATC	4 cut(s)	732, 855, 975, 1028
DpnII	GATC	4 cut(s)	730, 853, 973, 1026
EaeI	YGGCCR	3 cut(s)	192, 292, 808
EagI	CGGCCG	1 cut(s)	292
Eam1104I	CTCTTC	4 cut(s)	150, 540, 806, 1149
EarI	CTCTTC	4 cut(s)	150, 540, 806, 1149
EciI	GGCGGA	1 cut(s)	305
EclXI	CGGCCG	1 cut(s)	292
Eco130I	CCWWGG	1 cut(s)	179
Eco24I	GRGCYC	1 cut(s)	411
Eco52I	CGGCCG	1 cut(s)	292
Eco88I	CYCGRG	1 cut(s)	1094
EcoNI	CCTNNNNNAGG	1 cut(s)	370
EcoO109I	RGGNCCY	3 cut(s)	407, 408, 1305
EcoRII	CCWGG	3 cut(s)	563, 805, 849
EcoT14I	CCWWGG	1 cut(s)	179
EcoT22I	ATGCAT	1 cut(s)	1042
EcoT38I	GRGCYC	1 cut(s)	411
ErhI	CCWWGG	1 cut(s)	179
FaeI	CATG	5 cut(s)	183, 454, 526, 1189, 1297
FalI	AAGNNNNNCTT	2 cut(s)	804, 836
FaqI	GGGAC	2 cut(s)	356, 414
FatI	CATG	5 cut(s)	179, 450, 522, 1185, 1293
FauI	CCCGC	1 cut(s)	197
FblI	GTMKAC	1 cut(s)	270
Fnu4HI	GCNGC	4 cut(s)	55, 244, 319, 322
FokI	GGATG	2 cut(s)	196, 374
FriOI	GRGCYC	1 cut(s)	411
Fsp4HI	GCNGC	4 cut(s)	55, 244, 319, 322
FspBI	CTAG	3 cut(s)	969, 1133, 1257
GlaI	GCGC	2 cut(s)	841, 1184
GluI	GCNGC	4 cut(s)	55, 244, 319, 322
GsuI	CTGGAG	1 cut(s)	744
HaeIII	GGCC	5 cut(s)	194, 294, 409, 810, 1306
HapII	CCGG	2 cut(s)	291, 1179
HgaI	GACGC	3 cut(s)	60, 313, 1295
HhaI	GCGC	2 cut(s)	842, 1185
Hin1I	GRCGYC	2 cut(s)	52, 324
Hin1II	CATG	5 cut(s)	183, 454, 526, 1189, 1297
Hin6I	GCGC	2 cut(s)	840, 1183
HinP1I	GCGC	2 cut(s)	840, 1183
HincII	GTYRAC	1 cut(s)	271
HindII	GTYRAC	1 cut(s)	271
HindIII	AAGCTT	1 cut(s)	1123
HinfI	GANTC	6 cut(s)	175, 252, 355, 920, 1001, 1058
HpaII	CCGG	2 cut(s)	291, 1179
HphI	GGTGA	5 cut(s)	272, 280, 679, 1208, 1312
Hpy166II	GTNNAC	5 cut(s)	271, 396, 456, 1052, 1285
Hpy188I	TCNGA	4 cut(s)	24, 418, 537, 628
Hpy188III	TCNNGA	2 cut(s)	359, 1094
Hpy8I	GTNNAC	5 cut(s)	271, 396, 456, 1052, 1285
Hpy99I	CGWCG	3 cut(s)	87, 272, 512
HpyAV	CCTTC	5 cut(s)	145, 457, 625, 661, 1159
HpyCH4III	ACNGT	2 cut(s)	73, 541
HpyCH4IV	ACGT	1 cut(s)	306
HpyCH4V	TGCA	8 cut(s)	427, 469, 522, 763, 911, 943, 1007, 1040
HpyF10VI	GCNNNNNNNGC	4 cut(s)	282, 291, 433, 1295
HpyF3I	CTNAG	1 cut(s)	1121
HpySE526I	ACGT	1 cut(s)	306
Hsp92I	GRCGYC	2 cut(s)	52, 324
Hsp92II	CATG	5 cut(s)	183, 454, 526, 1189, 1297
HspAI	GCGC	2 cut(s)	840, 1183
KspI	CCGCGG	1 cut(s)	322
Kzo9I	GATC	4 cut(s)	730, 853, 973, 1026
LmnI	GCTCC	3 cut(s)	163, 889, 1085
Lsp1109I	GCAGC	1 cut(s)	230
LweI	GCATC	2 cut(s)	352, 445
MaeI	CTAG	3 cut(s)	969, 1133, 1257
MaeII	ACGT	1 cut(s)	306
MaeIII	GTNAC	4 cut(s)	109, 685, 994, 1267
MalI	GATC	4 cut(s)	732, 855, 975, 1028
MbiI	CCGCTC	1 cut(s)	490
MboI	GATC	4 cut(s)	730, 853, 973, 1026
MflI	RGATCY	1 cut(s)	853
MhlI	GDGCHC	1 cut(s)	411
MlsI	TGGCCA	1 cut(s)	810
MluCI	AATT	3 cut(s)	891, 979, 1014
MluNI	TGGCCA	1 cut(s)	810
MlyI	GAGTC	1 cut(s)	929
MmeI	TCCRAC	1 cut(s)	655
Mox20I	TGGCCA	1 cut(s)	810
Mph1103I	ATGCAT	1 cut(s)	1042
MscI	TGGCCA	1 cut(s)	810
MseI	TTAA	2 cut(s)	830, 1017
MslI	CAYNNNNRTG	1 cut(s)	1298
Msp20I	TGGCCA	1 cut(s)	810
MspA1I	CMGCKG	1 cut(s)	321
MspI	CCGG	2 cut(s)	291, 1179
MspR9I	CCNGG	4 cut(s)	565, 807, 851, 1180
Mva1269I	GAATGC	3 cut(s)	276, 577, 1289
MvaI	CCWGG	3 cut(s)	565, 807, 851
MvnI	CGCG	2 cut(s)	321, 842
MwoI	GCNNNNNNNGC	4 cut(s)	282, 291, 433, 1295
NciI	CCSGG	1 cut(s)	1180
NcoI	CCATGG	1 cut(s)	179
NdeII	GATC	4 cut(s)	730, 853, 973, 1026
NlaIII	CATG	5 cut(s)	183, 454, 526, 1189, 1297
NlaIV	GGNNCC	6 cut(s)	409, 486, 649, 855, 1177, 1307
NmuCI	GTSAC	3 cut(s)	109, 685, 1267
NsiI	ATGCAT	1 cut(s)	1042
NspI	RCATGY	2 cut(s)	526, 1189
PaeI	GCATGC	1 cut(s)	1189
PaeR7I	CTCGAG	1 cut(s)	1094
PctI	GAATGC	3 cut(s)	276, 577, 1289
PfeI	GAWTC	5 cut(s)	175, 252, 355, 1001, 1058
PflMI	CCANNNNNTGG	1 cut(s)	893
PkrI	GCNGC	4 cut(s)	56, 245, 320, 323
PleI	GAGTC	1 cut(s)	928
PpsI	GAGTC	1 cut(s)	928
Psp6I	CCWGG	3 cut(s)	563, 805, 849
PspGI	CCWGG	3 cut(s)	563, 805, 849
PspN4I	GGNNCC	6 cut(s)	409, 486, 649, 855, 1177, 1307
PspOMI	GGGCCC	1 cut(s)	407
PspPI	GGNCC	3 cut(s)	407, 408, 1305
PstI	CTGCAG	1 cut(s)	945
PsuI	RGATCY	1 cut(s)	853
RsaI	GTAC	2 cut(s)	395, 498
RsaNI	GTAC	2 cut(s)	394, 497
RseI	CAYNNNNRTG	1 cut(s)	1298
SacII	CCGCGG	1 cut(s)	322
SalI	GTCGAC	1 cut(s)	269
SaqAI	TTAA	2 cut(s)	830, 1017
SatI	GCNGC	4 cut(s)	55, 244, 319, 322
Sau3AI	GATC	4 cut(s)	730, 853, 973, 1026
Sau96I	GGNCC	3 cut(s)	407, 408, 1305
SbfI	CCTGCAGG	1 cut(s)	945
SchI	GAGTC	1 cut(s)	929
ScrFI	CCNGG	4 cut(s)	565, 807, 851, 1180
SdaI	CCTGCAGG	1 cut(s)	945
SduI	GDGCHC	1 cut(s)	411
SfaNI	GCATC	2 cut(s)	352, 445
SfcI	CTRYAG	1 cut(s)	941
Sfr274I	CTCGAG	1 cut(s)	1094
Sfr303I	CCGCGG	1 cut(s)	322
SgrBI	CCGCGG	1 cut(s)	322
SlaI	CTCGAG	1 cut(s)	1094
SmiMI	CAYNNNNRTG	1 cut(s)	1298
SmlI	CTYRAG	1 cut(s)	1094
SmoI	CTYRAG	1 cut(s)	1094
SphI	GCATGC	1 cut(s)	1189
Sse8387I	CCTGCAGG	1 cut(s)	945
Sse9I	AATT	3 cut(s)	891, 979, 1014
SsiI	CCGC	9 cut(s)	55, 204, 274, 283, 316, 319, 321, 488, 1298
SspI	AATATT	1 cut(s)	464
SspMI	CTAG	3 cut(s)	969, 1133, 1257
StyD4I	CCNGG	4 cut(s)	563, 805, 849, 1178
StyI	CCWWGG	1 cut(s)	179
TaaI	ACNGT	2 cut(s)	73, 541
TaiI	ACGT	1 cut(s)	309
TaqI	TCGA	6 cut(s)	213, 255, 270, 633, 1095, 1152
TasI	AATT	3 cut(s)	891, 979, 1014
TatI	WGTACW	2 cut(s)	393, 496
TauI	GCSGC	3 cut(s)	57, 321, 324
TfiI	GAWTC	5 cut(s)	175, 252, 355, 1001, 1058
Tru1I	TTAA	2 cut(s)	830, 1017
Tru9I	TTAA	2 cut(s)	830, 1017
TscAI	CASTG	2 cut(s)	78, 727
TseFI	GTSAC	3 cut(s)	109, 685, 1267
TseI	GCWGC	1 cut(s)	243
Tsp45I	GTSAC	3 cut(s)	109, 685, 1267
TspDTI	ATGAA	6 cut(s)	17, 439, 607, 777, 1282, 1312
TspGWI	ACGGA	3 cut(s)	100, 1177, 1244
TspRI	CASTG	2 cut(s)	78, 727
Van91I	CCANNNNNTGG	1 cut(s)	893
XagI	CCTNNNNNAGG	1 cut(s)	370
XapI	RAATTY	1 cut(s)	979
XceI	RCATGY	2 cut(s)	526, 1189
XhoI	CTCGAG	1 cut(s)	1094
XmiI	GTMKAC	1 cut(s)	270
XspI	CTAG	3 cut(s)	969, 1133, 1257
Zsp2I	ATGCAT	1 cut(s)	1042

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.