Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0001148

Physical Location & Seq

Genomic Coordinates Reverse (-)

43222538 .. 43227633

Gene Structure 5096 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G030440.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1209 bp

ATGGATATAAAGGAAAACGGAGAAGAAGACCAGCAGATTGTGAATCCTTGGAAGGTGGAGGCCAAAGAAGGCGGCAAAATTGACTATGATAAGCTCATAGATCAATTTGGTTGCCAGAGGATCGATCAGTCTCTTGTTGACCGTGTCCGCCGTCTCACCTCACGTGCTCCGCACGTTTTCCTCCGTCGTGATGTCTTCTTCGCTCACCGGGACTTTAATGAGATTCTGGATGCTTATGAGAGAGGAGAGAAGTTCTATTTGTACACAGGAAGAGGGCCTTCCTCTGAGGCTTTGCATTTGGGGCATCTCATCCCCTTCATGTTCACCAAATATTTGCAAGATGCCTTCAAGGTCCCTCTTGTTATACAACTTACCGACGACGAGAAATGTATGTGGAAAAATCTCACAGTTGAAGAAAGCCAAAGACTTGCAAGGGAAAATGCTAAAGACATCATTGCTTGTGGTTTTGACGTAACAAGAACCTTTATCTTCTCTGATTTTGATTATGTTGGTGGTGCCTTCTACAAGAACATGGTTAGGGTCGGAAAGTGTGTCACTTATAATAAGGTTGTTGGCATATTTGGTTTCACTGGTGAAGATCATATAGGAAAAGTTAGTTTTCCTCCTGTACAGGCGGTACCATCATTTCCAAGTTCATTTCCACATCTCTTCTCTGGCCAAGATAATCTTCGTTGTTTAATACCCTGTGCAATTGACCAGGATCCTTATTTTAGAATGACCCGAGATGTTGCTCCACGCATTGGATATCACAAACCTGCTTTAATCGAGTCTCTATTCTTCCCTGCCCTACAGGGAGAAACAGGAAAAATGTCGGCTAGTGATCCCAATTCTGCCATCTATGTACTGATTCTGCAAAGGATATTAAGAGCAAGGTTTGCATATGACATAAACAAGTATGCATTTTCAGGTGGACAAGATACAATAGAGAAACACAGAGAGTTGGGAGCAAATCTCGAGGTAGACATTCCATATAAATATCTGAGATTTTTTCTGGATGATGACATCGAGCTTGATTATATAGGAAAGGAGTATGGTGCAGGTCGAATGTTGACAGGGGAAGTTAAGCAGAGGCTGATTCAAGTTTTGACAGAAATTGTGGAGAGACATCGTAGGGCTAGAGCTGCCGTCACCGATGAGATGGTGGACGCATTTATGGCAGTGAGGCCCCTCCCAAACATGTTCAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004812 GO:0004830 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006139 GO:0006399 GO:0006412 GO:0006418 GO:0006436 GO:0006518 GO:0006520 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010467 GO:0016070 GO:0016874 GO:0016875 GO:0019538 GO:0019752 GO:0034641 GO:0034645 GO:0034660 GO:0043038 GO:0043039 GO:0043043 GO:0043170 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0140098 GO:0140101 GO:1901360 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00970 map00970

Pfam Domains

Protein Families

tRNA-synt_1b

Protein Analysis

402

Amino Acids

46.21

Weight (kDa)

6.04

Isoelectric Point (pI)

43.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
tRNA-synt_1b	PF00579	82 - 372	8.1e-14	tRNA synthetases class I (W and Y)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04600 AT3G04600 AT3G04600
fragaria_vesca	FvH4_4g11950 FvH4_4g11950 FvH4_4g11950 FvH4_4g11950 FvH4_5g27321
malus_domestica	MD04G1236500.v1.1 MD12G1255800.v1.1
prunus_persica	Prupe.7G057000_v2.0.a1 Prupe.7G057800_v2.0.a1
pyrus_communis	pycom04g21040 pycom12g23440
rosa_chinensis	RchiOBHm_Chr3g0493481 RchiOBHm_Chr7g0219201 RchiOBHm_Chr7g0219221 RchiOBHm_Chr7g0223011
rosa_laevigata	RLG00000002003 RLG00000002302 RLG00000002303 RLG00000022713
rosa_multiflora	Rmu_sc0000868.1_g000014 Rmu_sc0006373.1_g000002 Rmu_sc0006514.1_g000024 Rmu_sc0007422.1_g000005 Rmu_sc0009785.1_g000024 Rmu_sc0039697.1_g000001
rosa_roxburghii	Rroxscaffold_3G00234440 Rroxscaffold_3G00236350 Rroxscaffold_3G00237890 Rroxscaffold_3G00237970 Rroxscaffold_3G00238030 Rroxscaffold_3G00238960 Rroxscaffold_3G00238970 Rroxscaffold_5G00377610 Rroxscaffold_6G00391570 Rroxscaffold_7G00199590 Rroxscaffold_7G00199600
rosa_rugosa	Rorug07G0177500 Rorug07G0177500 Rorug07G0212800
rosa_samantha	Rh3AG310200 Rh3BG346300 Rh3CG344200 Rh3DG345400 Rh7AG319000 Rh7AG326700 Rh7AG357900 Rh7BG310900 Rh7BG346100 Rh7CG376000 Rh7DG318100 Rh7DG318200 Rh7DG352800
rosa_wichuraiana	Rw3G027260 Rw7G027160 Rw7G030440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	561
Acc36I	ACCTGC	2 cut(s)	784, 1049
Acc65I	GGTACC	1 cut(s)	637
AccB1I	GGYRCC	2 cut(s)	515, 637
AccB7I	CCANNNNNTGG	1 cut(s)	761
AccI	GTMKAC	1 cut(s)	981
AciI	CCGC	4 cut(s)	72, 148, 170, 635
AclWI	GGATC	4 cut(s)	128, 716, 729, 836
AcoI	YGGCCR	1 cut(s)	676
AcvI	CACGTG	1 cut(s)	164
AfaI	GTAC	4 cut(s)	263, 630, 639, 864
AfiI	CCNNNNNNNGG	1 cut(s)	761
AflIII	ACRYGT	1 cut(s)	1197
AgsI	TTSAA	4 cut(s)	349, 413, 1100, 1204
AjnI	CCWGG	1 cut(s)	717
AjuI	GAANNNNNNNTTGG	2 cut(s)	672, 704
AluBI	AGCT	3 cut(s)	94, 1030, 1142
AluI	AGCT	3 cut(s)	94, 1030, 1142
Alw21I	GWGCWC	1 cut(s)	169
Alw26I	GTCTC	4 cut(s)	135, 158, 795, 1117
AlwI	GGATC	4 cut(s)	128, 716, 729, 836
AlwNI	CAGNNNCTG	1 cut(s)	1093
Ama87I	CYCGRG	2 cut(s)	741, 974
AoxI	GGCC	4 cut(s)	60, 275, 676, 1184
ApeKI	GCWGC	1 cut(s)	1142
Asp718I	GGTACC	1 cut(s)	637
AspS9I	GGNCC	3 cut(s)	275, 352, 1185
AsuC2I	CCSGG	1 cut(s)	209
AsuHPI	GGTGA	5 cut(s)	148, 197, 316, 605, 1141
AvaI	CYCGRG	2 cut(s)	741, 974
AvaII	GGWCC	1 cut(s)	352
BalI	TGGCCA	1 cut(s)	678
BamHI	GGATCC	1 cut(s)	721
BanI	GGYRCC	2 cut(s)	515, 637
BbrPI	CACGTG	1 cut(s)	164
BbsI	GAAGAC	2 cut(s)	33, 187
Bbv12I	GWGCWC	1 cut(s)	169
BbvI	GCAGC	1 cut(s)	1129
BccI	CCATC	3 cut(s)	649, 863, 1153
BceAI	ACGGC	2 cut(s)	135, 1130
BciT130I	CCWGG	1 cut(s)	719
BcnI	CCSGG	1 cut(s)	209
BcoDI	GTCTC	4 cut(s)	135, 158, 795, 1117
BfaI	CTAG	2 cut(s)	837, 1137
BfmI	CTRYAG	1 cut(s)	809
BfuAI	ACCTGC	2 cut(s)	784, 1049
BisI	GCNGC	2 cut(s)	73, 1143
BlsI	GCNGC	2 cut(s)	74, 1144
Bme1390I	CCNGG	2 cut(s)	209, 719
Bme18I	GGWCC	1 cut(s)	352
BmeT110I	CYCGRG	2 cut(s)	741, 974
BmgT120I	GGNCC	3 cut(s)	275, 352, 1185
BmiI	GGNNCC	5 cut(s)	354, 517, 639, 723, 1187
BmrFI	CCNGG	2 cut(s)	209, 719
BmsI	GCATC	3 cut(s)	220, 313, 331
BpiI	GAAGAC	2 cut(s)	33, 187
BplI	GAGNNNNNCTC	2 cut(s)	957, 989
BpuMI	CCSGG	1 cut(s)	209
Bsa29I	ATCGAT	1 cut(s)	123
BsaAI	YACGTR	1 cut(s)	164
BsaJI	CCNNGG	1 cut(s)	47
Bsc4I	CCNNNNNNNGG	1 cut(s)	761
Bse1I	ACTGG	1 cut(s)	595
Bse3DI	GCAATG	1 cut(s)	453
BseBI	CCWGG	1 cut(s)	719
BseCI	ATCGAT	1 cut(s)	123
BseDI	CCNNGG	1 cut(s)	47
BseGI	GGATG	3 cut(s)	235, 309, 1021
BseLI	CCNNNNNNNGG	1 cut(s)	761
BseMI	GCAATG	1 cut(s)	453
BseMII	CTCAG	2 cut(s)	276, 992
BseNI	ACTGG	1 cut(s)	595
BseRI	GAGGAG	1 cut(s)	258
BseXI	GCAGC	1 cut(s)	1129
BsgI	GTGCAG	1 cut(s)	1077
BshFI	GGCC	4 cut(s)	62, 277, 678, 1186
BshNI	GGYRCC	2 cut(s)	515, 637
BshVI	ATCGAT	1 cut(s)	123
BsiHKAI	GWGCWC	1 cut(s)	169
BsiHKCI	CYCGRG	2 cut(s)	741, 974
BsiSI	CCGG	1 cut(s)	208
BslFI	GGGAC	2 cut(s)	224, 338
BslI	CCNNNNNNNGG	1 cut(s)	761
BsmAI	GTCTC	4 cut(s)	135, 158, 795, 1117
BsmBI	CGTCTC	1 cut(s)	158
BsmFI	GGGAC	2 cut(s)	224, 338
BsnI	GGCC	4 cut(s)	62, 277, 678, 1186
BsoBI	CYCGRG	2 cut(s)	741, 974
Bsp1286I	GDGCHC	1 cut(s)	169
Bsp1407I	TGTACA	2 cut(s)	261, 628
Bsp143I	GATC	6 cut(s)	100, 120, 124, 598, 721, 841
BspACI	CCGC	4 cut(s)	72, 148, 170, 635
BspANI	GGCC	4 cut(s)	62, 277, 678, 1186
BspCNI	CTCAG	2 cut(s)	277, 993
BspDI	ATCGAT	1 cut(s)	123
BspLI	GGNNCC	5 cut(s)	354, 517, 639, 723, 1187
BspMI	ACCTGC	2 cut(s)	784, 1049
BspPI	GGATC	4 cut(s)	128, 716, 729, 836
BspT107I	GGYRCC	2 cut(s)	515, 637
BsrDI	GCAATG	1 cut(s)	453
BsrGI	TGTACA	2 cut(s)	261, 628
BsrI	ACTGG	1 cut(s)	595
BssECI	CCNNGG	1 cut(s)	47
BssMI	GATC	6 cut(s)	100, 120, 124, 598, 721, 841
BssT1I	CCWWGG	1 cut(s)	47
Bst2UI	CCWGG	1 cut(s)	719
Bst4CI	ACNGT	2 cut(s)	143, 409
Bst6I	CTCTTC	2 cut(s)	265, 674
BstAPI	GCANNNNNTGC	1 cut(s)	896
BstAUI	TGTACA	2 cut(s)	261, 628
BstBAI	YACGTR	1 cut(s)	164
BstDEI	CTNAG	2 cut(s)	285, 1001
BstF5I	GGATG	3 cut(s)	235, 309, 1021
BstKTI	GATC	6 cut(s)	103, 123, 127, 601, 724, 844
BstMAI	GTCTC	4 cut(s)	135, 158, 795, 1117
BstMBI	GATC	6 cut(s)	100, 120, 124, 598, 721, 841
BstMWI	GCNNNNNNNGC	4 cut(s)	301, 896, 1142, 1175
BstNI	CCWGG	1 cut(s)	719
BstNSI	RCATGY	1 cut(s)	1201
BstSCI	CCNGG	2 cut(s)	207, 717
BstSFI	CTRYAG	1 cut(s)	809
BstV1I	GCAGC	1 cut(s)	1129
BstV2I	GAAGAC	2 cut(s)	33, 187
BstX2I	RGATCY	1 cut(s)	721
BstYI	RGATCY	1 cut(s)	721
Bsu15I	ATCGAT	1 cut(s)	123
BsuRI	GGCC	4 cut(s)	62, 277, 678, 1186
BsuTUI	ATCGAT	1 cut(s)	123
BtsCI	GGATG	3 cut(s)	235, 309, 1021
BtsI	GCAGTG	1 cut(s)	1185
BtsIMutI	CAGTG	2 cut(s)	588, 1185
BveI	ACCTGC	2 cut(s)	784, 1049
CaiI	CAGNNNCTG	1 cut(s)	1093
Cfr13I	GGNCC	3 cut(s)	275, 352, 1185
ClaI	ATCGAT	1 cut(s)	123
CseI	GACGC	1 cut(s)	1175
Csp6I	GTAC	4 cut(s)	262, 629, 638, 863
CviAII	CATG	3 cut(s)	319, 532, 1198
CviQI	GTAC	4 cut(s)	262, 629, 638, 863
DdeI	CTNAG	2 cut(s)	285, 1001
DpnI	GATC	6 cut(s)	102, 122, 126, 600, 723, 843
DpnII	GATC	6 cut(s)	100, 120, 124, 598, 721, 841
EaeI	YGGCCR	1 cut(s)	676
Eam1104I	CTCTTC	2 cut(s)	265, 674
EarI	CTCTTC	2 cut(s)	265, 674
EciI	GGCGGA	1 cut(s)	137
Eco130I	CCWWGG	1 cut(s)	47
Eco32I	GATATC	1 cut(s)	767
Eco47I	GGWCC	1 cut(s)	352
Eco72I	CACGTG	1 cut(s)	164
Eco88I	CYCGRG	2 cut(s)	741, 974
EcoO109I	RGGNCCY	3 cut(s)	275, 352, 1185
EcoRII	CCWGG	1 cut(s)	717
EcoRV	GATATC	1 cut(s)	767
EcoT14I	CCWWGG	1 cut(s)	47
EcoT22I	ATGCAT	1 cut(s)	922
ErhI	CCWWGG	1 cut(s)	47
Esp3I	CGTCTC	1 cut(s)	158
FaeI	CATG	3 cut(s)	322, 535, 1201
FalI	AAGNNNNNCTT	4 cut(s)	262, 294, 672, 704
FaqI	GGGAC	2 cut(s)	224, 338
FatI	CATG	3 cut(s)	318, 531, 1197
FauNDI	CATATG	1 cut(s)	901
FblI	GTMKAC	1 cut(s)	981
Fnu4HI	GCNGC	2 cut(s)	73, 1143
FokI	GGATG	3 cut(s)	242, 296, 1028
Fsp4HI	GCNGC	2 cut(s)	73, 1143
FspBI	CTAG	2 cut(s)	837, 1137
GluI	GCNGC	2 cut(s)	73, 1143
HaeIII	GGCC	4 cut(s)	62, 277, 678, 1186
HapII	CCGG	1 cut(s)	208
HgaI	GACGC	1 cut(s)	1175
Hin1II	CATG	3 cut(s)	322, 535, 1201
HincII	GTYRAC	2 cut(s)	139, 1071
HindII	GTYRAC	2 cut(s)	139, 1071
HinfI	GANTC	5 cut(s)	43, 223, 788, 868, 1096
HpaII	CCGG	1 cut(s)	208
HphI	GGTGA	5 cut(s)	148, 197, 316, 605, 1141
Hpy166II	GTNNAC	7 cut(s)	139, 264, 324, 932, 982, 1071, 1165
Hpy188I	TCNGA	4 cut(s)	286, 496, 545, 1002
Hpy188III	TCNNGA	4 cut(s)	188, 227, 974, 1013
Hpy8I	GTNNAC	7 cut(s)	139, 264, 324, 932, 982, 1071, 1165
Hpy99I	CGWCG	3 cut(s)	189, 380, 383
HpyAV	CCTTC	6 cut(s)	46, 62, 288, 325, 355, 529
HpyCH4III	ACNGT	2 cut(s)	143, 409
HpyCH4IV	ACGT	3 cut(s)	163, 174, 471
HpyCH4V	TGCA	8 cut(s)	295, 337, 431, 710, 874, 899, 920, 1058
HpyF10VI	GCNNNNNNNGC	4 cut(s)	301, 896, 1142, 1175
HpyF3I	CTNAG	2 cut(s)	285, 1001
HpySE526I	ACGT	3 cut(s)	163, 174, 471
Hsp92II	CATG	3 cut(s)	322, 535, 1201
KpnI	GGTACC	1 cut(s)	641
Kzo9I	GATC	6 cut(s)	100, 120, 124, 598, 721, 841
LmnI	GCTCC	3 cut(s)	172, 757, 965
Lsp1109I	GCAGC	1 cut(s)	1129
LweI	GCATC	3 cut(s)	220, 313, 331
MaeI	CTAG	2 cut(s)	837, 1137
MaeII	ACGT	3 cut(s)	163, 174, 471
MaeIII	GTNAC	3 cut(s)	472, 553, 1147
MalI	GATC	6 cut(s)	102, 122, 126, 600, 723, 843
MboI	GATC	6 cut(s)	100, 120, 124, 598, 721, 841
MfeI	CAATTG	1 cut(s)	711
MflI	RGATCY	1 cut(s)	721
MhlI	GDGCHC	1 cut(s)	169
MlsI	TGGCCA	1 cut(s)	678
MluCI	AATT	5 cut(s)	78, 104, 711, 847, 1113
MluNI	TGGCCA	1 cut(s)	678
MlyI	GAGTC	1 cut(s)	797
MmeI	TCCRAC	1 cut(s)	523
Mox20I	TGGCCA	1 cut(s)	678
Mph1103I	ATGCAT	1 cut(s)	922
MscI	TGGCCA	1 cut(s)	678
MseI	TTAA	5 cut(s)	216, 698, 782, 884, 1083
Msp20I	TGGCCA	1 cut(s)	678
MspI	CCGG	1 cut(s)	208
MspR9I	CCNGG	2 cut(s)	209, 719
MunI	CAATTG	1 cut(s)	711
MvaI	CCWGG	1 cut(s)	719
MwoI	GCNNNNNNNGC	4 cut(s)	301, 896, 1142, 1175
NciI	CCSGG	1 cut(s)	209
NdeI	CATATG	1 cut(s)	901
NdeII	GATC	6 cut(s)	100, 120, 124, 598, 721, 841
NlaIII	CATG	3 cut(s)	322, 535, 1201
NlaIV	GGNNCC	5 cut(s)	354, 517, 639, 723, 1187
NmuCI	GTSAC	2 cut(s)	553, 1147
NsiI	ATGCAT	1 cut(s)	922
NspI	RCATGY	1 cut(s)	1201
PaeR7I	CTCGAG	1 cut(s)	974
PciI	ACATGT	1 cut(s)	1197
PfeI	GAWTC	4 cut(s)	43, 223, 868, 1096
PflFI	GACNNNGTC	1 cut(s)	143
PflMI	CCANNNNNTGG	1 cut(s)	761
PkrI	GCNGC	2 cut(s)	74, 1144
PleI	GAGTC	1 cut(s)	796
PmaCI	CACGTG	1 cut(s)	164
PmlI	CACGTG	1 cut(s)	164
PpsI	GAGTC	1 cut(s)	796
Ppu21I	YACGTR	1 cut(s)	164
PpuMI	RGGWCCY	1 cut(s)	352
PscI	ACATGT	1 cut(s)	1197
PsiI	TTATAA	1 cut(s)	561
Psp5II	RGGWCCY	1 cut(s)	352
Psp6I	CCWGG	1 cut(s)	717
PspCI	CACGTG	1 cut(s)	164
PspGI	CCWGG	1 cut(s)	717
PspN4I	GGNNCC	5 cut(s)	354, 517, 639, 723, 1187
PspPI	GGNCC	3 cut(s)	275, 352, 1185
PspPPI	RGGWCCY	1 cut(s)	352
PstNI	CAGNNNCTG	1 cut(s)	1093
PsuI	RGATCY	1 cut(s)	721
PsyI	GACNNNGTC	1 cut(s)	143
RsaI	GTAC	4 cut(s)	263, 630, 639, 864
RsaNI	GTAC	4 cut(s)	262, 629, 638, 863
SaqAI	TTAA	5 cut(s)	216, 698, 782, 884, 1083
SatI	GCNGC	2 cut(s)	73, 1143
Sau3AI	GATC	6 cut(s)	100, 120, 124, 598, 721, 841
Sau96I	GGNCC	3 cut(s)	275, 352, 1185
SchI	GAGTC	1 cut(s)	797
ScrFI	CCNGG	2 cut(s)	209, 719
SduI	GDGCHC	1 cut(s)	169
SfaNI	GCATC	3 cut(s)	220, 313, 331
SfcI	CTRYAG	1 cut(s)	809
Sfr274I	CTCGAG	1 cut(s)	974
SinI	GGWCC	1 cut(s)	352
SlaI	CTCGAG	1 cut(s)	974
SmlI	CTYRAG	1 cut(s)	974
SmoI	CTYRAG	1 cut(s)	974
Sse9I	AATT	5 cut(s)	78, 104, 711, 847, 1113
SsiI	CCGC	4 cut(s)	72, 148, 170, 635
SspI	AATATT	1 cut(s)	332
SspMI	CTAG	2 cut(s)	837, 1137
StyD4I	CCNGG	2 cut(s)	207, 717
StyI	CCWWGG	1 cut(s)	47
TaaI	ACNGT	2 cut(s)	143, 409
TaiI	ACGT	3 cut(s)	166, 177, 474
TaqI	TCGA	5 cut(s)	123, 786, 975, 1026, 1063
TasI	AATT	5 cut(s)	78, 104, 711, 847, 1113
TatI	WGTACW	3 cut(s)	261, 628, 862
TauI	GCSGC	1 cut(s)	75
TfiI	GAWTC	4 cut(s)	43, 223, 868, 1096
Tru1I	TTAA	5 cut(s)	216, 698, 782, 884, 1083
Tru9I	TTAA	5 cut(s)	216, 698, 782, 884, 1083
TscAI	CASTG	2 cut(s)	595, 1185
TseFI	GTSAC	2 cut(s)	553, 1147
TseI	GCWGC	1 cut(s)	1142
Tsp45I	GTSAC	2 cut(s)	553, 1147
TspDTI	ATGAA	2 cut(s)	307, 645
TspGWI	ACGGA	2 cut(s)	33, 173
TspRI	CASTG	2 cut(s)	595, 1185
Tth111I	GACNNNGTC	1 cut(s)	143
Van91I	CCANNNNNTGG	1 cut(s)	761
VpaK11BI	GGWCC	1 cut(s)	352
XceI	RCATGY	1 cut(s)	1201
XhoI	CTCGAG	1 cut(s)	974
XmiI	GTMKAC	1 cut(s)	981
XspI	CTAG	2 cut(s)	837, 1137
Zsp2I	ATGCAT	1 cut(s)	922

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.