Belongs to the class-I aminoacyl-tRNA synthetase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 197 |
| AgsI | TTSAA | 1 cut(s) | 31 |
| Alw26I | GTCTC | 1 cut(s) | 100 |
| AsuHPI | GGTGA | 3 cut(s) | 79, 229, 287 |
| BanI | GGYRCC | 1 cut(s) | 197 |
| BcoDI | GTCTC | 1 cut(s) | 100 |
| BfaI | CTAG | 2 cut(s) | 114, 333 |
| BmiI | GGNNCC | 2 cut(s) | 36, 199 |
| BmsI | GCATC | 1 cut(s) | 13 |
| BsaJI | CCNNGG | 1 cut(s) | 90 |
| BsaWI | WCCGGW | 1 cut(s) | 356 |
| Bse1I | ACTGG | 1 cut(s) | 277 |
| BseDI | CCNNGG | 1 cut(s) | 90 |
| BseNI | ACTGG | 1 cut(s) | 277 |
| BshNI | GGYRCC | 1 cut(s) | 197 |
| BsiSI | CCGG | 1 cut(s) | 357 |
| BsmAI | GTCTC | 1 cut(s) | 100 |
| BsmI | GAATGC | 1 cut(s) | 127 |
| Bsp143I | GATC | 1 cut(s) | 280 |
| BspLI | GGNNCC | 2 cut(s) | 36, 199 |
| BspT107I | GGYRCC | 1 cut(s) | 197 |
| BsrI | ACTGG | 1 cut(s) | 277 |
| BssECI | CCNNGG | 1 cut(s) | 90 |
| BssMI | GATC | 1 cut(s) | 280 |
| Bst4CI | ACNGT | 1 cut(s) | 91 |
| BstC8I | GCNNGC | 1 cut(s) | 256 |
| BstDSI | CCRYGG | 1 cut(s) | 90 |
| BstKTI | GATC | 1 cut(s) | 283 |
| BstMAI | GTCTC | 1 cut(s) | 100 |
| BstMBI | GATC | 1 cut(s) | 280 |
| BstNSI | RCATGY | 1 cut(s) | 76 |
| BtgI | CCRYGG | 1 cut(s) | 90 |
| BtsIMutI | CAGTG | 2 cut(s) | 54, 270 |
| Cac8I | GCNNGC | 1 cut(s) | 256 |
| CviAII | CATG | 2 cut(s) | 73, 214 |
| CviJI | RGCY | 2 cut(s) | 102, 147 |
| CviKI_1 | RGCY | 2 cut(s) | 102, 147 |
| DpnI | GATC | 1 cut(s) | 282 |
| DpnII | GATC | 1 cut(s) | 280 |
| FaeI | CATG | 2 cut(s) | 76, 217 |
| FaiI | YATR | 8 cut(s) | 47, 74, 155, 189, 215, 260, 285, 287 |
| FatI | CATG | 2 cut(s) | 72, 213 |
| FspBI | CTAG | 2 cut(s) | 114, 333 |
| HapII | CCGG | 1 cut(s) | 357 |
| Hin1II | CATG | 2 cut(s) | 76, 217 |
| HpaII | CCGG | 1 cut(s) | 357 |
| HphI | GGTGA | 3 cut(s) | 79, 229, 287 |
| Hpy166II | GTNNAC | 1 cut(s) | 6 |
| Hpy188III | TCNNGA | 1 cut(s) | 362 |
| Hpy8I | GTNNAC | 1 cut(s) | 6 |
| HpyAV | CCTTC | 4 cut(s) | 37, 211, 214, 379 |
| HpyCH4III | ACNGT | 1 cut(s) | 91 |
| HpyCH4V | TGCA | 2 cut(s) | 19, 72 |
| Hsp92II | CATG | 2 cut(s) | 76, 217 |
| Kzo9I | GATC | 1 cut(s) | 280 |
| LpnPI | CCDG | 5 cut(s) | 116, 240, 258, 299, 370 |
| LweI | GCATC | 1 cut(s) | 13 |
| MaeI | CTAG | 2 cut(s) | 114, 333 |
| MaeIII | GTNAC | 1 cut(s) | 235 |
| MalI | GATC | 1 cut(s) | 282 |
| MboI | GATC | 1 cut(s) | 280 |
| MboII | GAAGA | 3 cut(s) | 107, 163, 290 |
| MluCI | AATT | 1 cut(s) | 178 |
| MseI | TTAA | 1 cut(s) | 379 |
| MspI | CCGG | 1 cut(s) | 357 |
| Mva1269I | GAATGC | 1 cut(s) | 127 |
| NdeII | GATC | 1 cut(s) | 280 |
| NlaIII | CATG | 2 cut(s) | 76, 217 |
| NlaIV | GGNNCC | 2 cut(s) | 36, 199 |
| NmuCI | GTSAC | 1 cut(s) | 235 |
| NspI | RCATGY | 1 cut(s) | 76 |
| PctI | GAATGC | 1 cut(s) | 127 |
| PspN4I | GGNNCC | 2 cut(s) | 36, 199 |
| SaqAI | TTAA | 1 cut(s) | 379 |
| Sau3AI | GATC | 1 cut(s) | 280 |
| SetI | ASST | 5 cut(s) | 36, 225, 242, 252, 371 |
| SfaNI | GCATC | 1 cut(s) | 13 |
| Sse9I | AATT | 1 cut(s) | 178 |
| SspI | AATATT | 1 cut(s) | 14 |
| SspMI | CTAG | 2 cut(s) | 114, 333 |
| TaaI | ACNGT | 1 cut(s) | 91 |
| TasI | AATT | 1 cut(s) | 178 |
| Tru1I | TTAA | 1 cut(s) | 379 |
| Tru9I | TTAA | 1 cut(s) | 379 |
| TscAI | CASTG | 2 cut(s) | 61, 277 |
| TseFI | GTSAC | 1 cut(s) | 235 |
| Tsp45I | GTSAC | 1 cut(s) | 235 |
| TspDTI | ATGAA | 2 cut(s) | 157, 327 |
| TspGWI | ACGGA | 1 cut(s) | 312 |
| TspRI | CASTG | 2 cut(s) | 61, 277 |
| XceI | RCATGY | 1 cut(s) | 76 |
| XspI | CTAG | 2 cut(s) | 114, 333 |