MD05G1173600.v1.1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001964
Physical Location & Seq
Reverse (-)
30209726 .. 30211965
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1173600.v1.1.491

Sequence Viewer

Length: 702 bp
ATGATAACCTGGCATTCTCTGCTATTTTTGCTAGTACTAGTTGTACTAGTTGTACTAGTTGTCAGAAAGCATGATGTAGGCGAAGCAGCTGAGGACGACGTTAGTTTTGACCAAAACTACGTTGTCAGATATGGAAATGATCATTTCTTGAAACTAAACCAAGGAAGAGATGTTCATATTTCTTTGAACCAAACTACGGGATCTGGATTTAATTCCAAGAACGGATATGGTTCGGGTTTCTTTGAAATGAAGCTGAAATTGCCATCAAGCCATTCTCCAGGAGTTGTCACAACATTTTATTTAACTTCACATCCAGATAATAAGCCTGGAAACCATGACGAGCTAGACTTCGAGTTCTTGGGTACCGATGGGCCAAATTATACCTTACAAACAAATATTTTTGCAAATGACAATGGGGAGAGAGAGCAGAGGCTTCATCTTCGGTTTGACCCCACCGAGGATTTTCATAATTATGGAATCCTATGGAACTATCGCCAAGTAGTAGTGTTCAAGAACAATACAATGTTGGGAGCAAGGTACCCTGAACTGAAGATGTTTGTGGAAGGAAGCACATGGAATGGAGAATCTTGGGCATCACATGGCAGAAAACAAAGATCTTATGAGAATGTGACTCAGAAATATCTGTTTTATGATTATTGTTCTCTCAGAAGAGAAAAATTCAAGGAATGCCAAGTGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

234

Amino Acids

27.06

Weight (kDa)

6.36

Isoelectric Point (pI)

26.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 37 - 202 5.9e-38 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 362, 537
AccB1I GGYRCC 2 cut(s) 362, 537
AclWI GGATC 1 cut(s) 208
AcsI RAATTY 1 cut(s) 677
AcuI CTGAAG 1 cut(s) 569
AfaI GTAC 5 cut(s) 36, 45, 54, 364, 539
AfiI CCNNNNNNNGG 2 cut(s) 196, 457
AgsI TTSAA 5 cut(s) 151, 187, 245, 511, 682
AhlI ACTAGT 3 cut(s) 37, 46, 55
AjnI CCWGG 3 cut(s) 8, 277, 325
AloI GAACNNNNNNTCC 2 cut(s) 156, 188
AluBI AGCT 3 cut(s) 89, 253, 343
AluI AGCT 3 cut(s) 89, 253, 343
AlwI GGATC 1 cut(s) 208
AoxI GGCC 1 cut(s) 371
ApeKI GCWGC 1 cut(s) 86
ApoI RAATTY 1 cut(s) 677
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
Asp718I GGTACC 2 cut(s) 362, 537
AspS9I GGNCC 1 cut(s) 371
BanI GGYRCC 2 cut(s) 362, 537
BbvCI CCTCAGC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 98
BccI CCATC 2 cut(s) 271, 362
BciT130I CCWGG 3 cut(s) 10, 279, 327
BclI TGATCA 1 cut(s) 139
BcuI ACTAGT 3 cut(s) 37, 46, 55
BfaI CTAG 5 cut(s) 32, 38, 47, 56, 344
BglII AGATCT 1 cut(s) 614
BisI GCNGC 1 cut(s) 87
BlsI GCNGC 1 cut(s) 88
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 3 cut(s) 10, 279, 327
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 2 cut(s) 364, 539
BmrFI CCNGG 3 cut(s) 10, 279, 327
BmsI GCATC 1 cut(s) 602
BpmI CTGGAG 1 cut(s) 261
Bpu10I CCTNAGC 1 cut(s) 90
BsaJI CCNNGG 2 cut(s) 160, 456
Bsc4I CCNNNNNNNGG 2 cut(s) 196, 457
BseBI CCWGG 3 cut(s) 10, 279, 327
BseDI CCNNGG 2 cut(s) 160, 456
BseGI GGATG 1 cut(s) 310
BseLI CCNNNNNNNGG 2 cut(s) 196, 457
BseMII CTCAG 3 cut(s) 81, 647, 679
BseXI GCAGC 1 cut(s) 98
BshFI GGCC 1 cut(s) 373
BshNI GGYRCC 2 cut(s) 362, 537
BslI CCNNNNNNNGG 2 cut(s) 196, 457
BsmI GAATGC 2 cut(s) 13, 692
BsnI GGCC 1 cut(s) 373
Bsp143I GATC 3 cut(s) 139, 200, 614
BspANI GGCC 1 cut(s) 373
BspCNI CTCAG 3 cut(s) 82, 646, 678
BspLI GGNNCC 2 cut(s) 364, 539
BspPI GGATC 1 cut(s) 208
BspT107I GGYRCC 2 cut(s) 362, 537
BssECI CCNNGG 2 cut(s) 160, 456
BssMI GATC 3 cut(s) 139, 200, 614
BssT1I CCWWGG 1 cut(s) 160
Bst2UI CCWGG 3 cut(s) 10, 279, 327
Bst6I CTCTTC 2 cut(s) 160, 664
BstAPI GCANNNNNTGC 1 cut(s) 19
BstDEI CTNAG 3 cut(s) 90, 633, 665
BstF5I GGATG 1 cut(s) 310
BstKTI GATC 3 cut(s) 142, 203, 617
BstMBI GATC 3 cut(s) 139, 200, 614
BstMWI GCNNNNNNNGC 3 cut(s) 19, 28, 259
BstNI CCWGG 3 cut(s) 10, 279, 327
BstSCI CCNGG 3 cut(s) 8, 277, 325
BstV1I GCAGC 1 cut(s) 98
BstX2I RGATCY 2 cut(s) 200, 614
BstYI RGATCY 2 cut(s) 200, 614
BsuRI GGCC 1 cut(s) 373
BtsCI GGATG 1 cut(s) 310
Cfr13I GGNCC 1 cut(s) 371
Csp6I GTAC 5 cut(s) 35, 44, 53, 363, 538
CviAII CATG 4 cut(s) 71, 335, 573, 599
CviJI RGCY 7 cut(s) 89, 253, 270, 325, 343, 373, 433
CviKI_1 RGCY 7 cut(s) 89, 253, 270, 325, 343, 373, 433
CviQI GTAC 5 cut(s) 35, 44, 53, 363, 538
DdeI CTNAG 3 cut(s) 90, 633, 665
DpnI GATC 3 cut(s) 141, 202, 616
DpnII GATC 3 cut(s) 139, 200, 614
Eam1104I CTCTTC 2 cut(s) 160, 664
EarI CTCTTC 2 cut(s) 160, 664
Eco130I CCWWGG 1 cut(s) 160
Eco57I CTGAAG 1 cut(s) 569
EcoRII CCWGG 3 cut(s) 8, 277, 325
EcoT14I CCWWGG 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 4 cut(s) 74, 338, 576, 602
FatI CATG 4 cut(s) 70, 334, 572, 598
FbaI TGATCA 1 cut(s) 139
Fnu4HI GCNGC 1 cut(s) 87
FokI GGATG 1 cut(s) 297
Fsp4HI GCNGC 1 cut(s) 87
FspBI CTAG 5 cut(s) 32, 38, 47, 56, 344
GluI GCNGC 1 cut(s) 87
GsuI CTGGAG 1 cut(s) 261
HaeIII GGCC 1 cut(s) 373
Hin1II CATG 4 cut(s) 74, 338, 576, 602
HinfI GANTC 3 cut(s) 477, 584, 631
Hpy188I TCNGA 4 cut(s) 65, 128, 636, 668
Hpy188III TCNNGA 4 cut(s) 148, 204, 314, 511
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 557
HpyCH4IV ACGT 2 cut(s) 99, 120
HpyCH4V TGCA 1 cut(s) 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 19, 28, 259
HpyF3I CTNAG 3 cut(s) 90, 633, 665
HpySE526I ACGT 2 cut(s) 99, 120
Hsp92II CATG 4 cut(s) 74, 338, 576, 602
KpnI GGTACC 2 cut(s) 366, 541
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 3 cut(s) 139, 200, 614
LmnI GCTCC 1 cut(s) 530
LpnPI CCDG 8 cut(s) 22, 189, 264, 291, 312, 327, 339, 555
Lsp1109I GCAGC 1 cut(s) 98
LweI GCATC 1 cut(s) 602
MaeI CTAG 5 cut(s) 32, 38, 47, 56, 344
MaeII ACGT 2 cut(s) 99, 120
MaeIII GTNAC 2 cut(s) 286, 628
MalI GATC 3 cut(s) 141, 202, 616
MboI GATC 3 cut(s) 139, 200, 614
MboII GAAGA 4 cut(s) 177, 431, 562, 681
MflI RGATCY 2 cut(s) 200, 614
MluCI AATT 5 cut(s) 211, 257, 376, 469, 677
MlyI GAGTC 1 cut(s) 625
MnlI CCTC 3 cut(s) 85, 423, 451
MseI TTAA 2 cut(s) 210, 302
MslI CAYNNNNRTG 1 cut(s) 471
MspA1I CMGCKG 1 cut(s) 89
MspR9I CCNGG 3 cut(s) 10, 279, 327
Mva1269I GAATGC 2 cut(s) 13, 692
MvaI CCWGG 3 cut(s) 10, 279, 327
MwoI GCNNNNNNNGC 3 cut(s) 19, 28, 259
NdeII GATC 3 cut(s) 139, 200, 614
NlaIII CATG 4 cut(s) 74, 338, 576, 602
NlaIV GGNNCC 2 cut(s) 364, 539
NmuCI GTSAC 2 cut(s) 286, 628
PctI GAATGC 2 cut(s) 13, 692
PfeI GAWTC 2 cut(s) 477, 584
PfoI TCCNGGA 1 cut(s) 277
PkrI GCNGC 1 cut(s) 88
PleI GAGTC 1 cut(s) 625
PpsI GAGTC 1 cut(s) 625
Psp6I CCWGG 3 cut(s) 8, 277, 325
PspGI CCWGG 3 cut(s) 8, 277, 325
PspN4I GGNNCC 2 cut(s) 364, 539
PspPI GGNCC 1 cut(s) 371
PsuI RGATCY 2 cut(s) 200, 614
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 5 cut(s) 36, 45, 54, 364, 539
RsaNI GTAC 5 cut(s) 35, 44, 53, 363, 538
RseI CAYNNNNRTG 1 cut(s) 471
SaqAI TTAA 2 cut(s) 210, 302
SatI GCNGC 1 cut(s) 87
Sau3AI GATC 3 cut(s) 139, 200, 614
Sau96I GGNCC 1 cut(s) 371
ScaI AGTACT 1 cut(s) 36
SchI GAGTC 1 cut(s) 625
ScrFI CCNGG 3 cut(s) 10, 279, 327
SetI ASST 8 cut(s) 11, 91, 102, 123, 255, 345, 386, 539
SfaNI GCATC 1 cut(s) 602
SmiMI CAYNNNNRTG 1 cut(s) 471
SpeI ACTAGT 3 cut(s) 37, 46, 55
Sse9I AATT 5 cut(s) 211, 257, 376, 469, 677
SspI AATATT 1 cut(s) 397
SspMI CTAG 5 cut(s) 32, 38, 47, 56, 344
StyD4I CCNGG 3 cut(s) 8, 277, 325
StyI CCWWGG 1 cut(s) 160
TaiI ACGT 2 cut(s) 102, 123
TaqI TCGA 1 cut(s) 351
TasI AATT 5 cut(s) 211, 257, 376, 469, 677
TatI WGTACW 3 cut(s) 34, 43, 52
TfiI GAWTC 2 cut(s) 477, 584
Tru1I TTAA 2 cut(s) 210, 302
Tru9I TTAA 2 cut(s) 210, 302
TseFI GTSAC 2 cut(s) 286, 628
TseI GCWGC 1 cut(s) 86
Tsp45I GTSAC 2 cut(s) 286, 628
TspDTI ATGAA 4 cut(s) 164, 263, 425, 455
TspGWI ACGGA 1 cut(s) 237
XapI RAATTY 1 cut(s) 677
XspI CTAG 5 cut(s) 32, 38, 47, 56, 344
ZrmI AGTACT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.