Rh6DG271100

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001964
Physical Location & Seq
Reverse (-)
46438300 .. 46444279
5980 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG271100.1

Sequence Viewer

Length: 324 bp
ATGATAATAGACGCCAGCATATGGGATGGATCAAATTGGGAAACTGATGGAGGCCAAACAAAGATCGACTGGAACCATGCACTATTTAGAGCTTACTTCCAAGGGTTTGACATAAGTTCTGGCTGTACACTAGATAAGTCCAGGACCGATGTTAAGGAATGTTATGCATCCACATACTTGTGGAACCATCAGGAGTATTGGCAACTAAACTCAACCGAACAACAAGCATATGAGAAGGTGAGAAAGGAACAGATGAACTATGACTACTGTACTGATTCAACAAGGTTCCCTACACCTCCCCCAGAGTGCAGTAGTAATACCTAA

Protein Analysis

107

Amino Acids

12.54

Weight (kDa)

4.58

Isoelectric Point (pI)

52.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
XET_C PF06955 58 - 103 3.7e-16 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 21
AclWI GGATC 1 cut(s) 37
AcyI GRCGYC 1 cut(s) 12
AfaI GTAC 2 cut(s) 127, 271
AfiI CCNNNNNNNGG 1 cut(s) 21
AgsI TTSAA 1 cut(s) 279
AjnI CCWGG 1 cut(s) 140
AluBI AGCT 1 cut(s) 92
AluI AGCT 1 cut(s) 92
AlwI GGATC 1 cut(s) 37
AoxI GGCC 1 cut(s) 52
AspS9I GGNCC 1 cut(s) 144
AsuHPI GGTGA 1 cut(s) 250
AvaII GGWCC 1 cut(s) 144
BccI CCATC 3 cut(s) 20, 41, 195
BciT130I CCWGG 1 cut(s) 142
BfaI CTAG 1 cut(s) 131
Bme1390I CCNGG 1 cut(s) 142
Bme18I GGWCC 1 cut(s) 144
BmgT120I GGNCC 1 cut(s) 144
BmiI GGNNCC 3 cut(s) 74, 185, 287
BmrFI CCNGG 1 cut(s) 142
BmsI GCATC 1 cut(s) 176
BsaHI GRCGYC 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 100
Bsc4I CCNNNNNNNGG 1 cut(s) 21
Bse1I ACTGG 1 cut(s) 74
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 2 cut(s) 31, 167
BseLI CCNNNNNNNGG 1 cut(s) 21
BseNI ACTGG 1 cut(s) 74
BshFI GGCC 1 cut(s) 54
BslI CCNNNNNNNGG 1 cut(s) 21
BsnI GGCC 1 cut(s) 54
Bsp1407I TGTACA 1 cut(s) 125
Bsp143I GATC 2 cut(s) 29, 63
BspANI GGCC 1 cut(s) 54
BspLI GGNNCC 3 cut(s) 74, 185, 287
BspPI GGATC 1 cut(s) 37
BsrGI TGTACA 1 cut(s) 125
BsrI ACTGG 1 cut(s) 74
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 2 cut(s) 29, 63
BssNI GRCGYC 1 cut(s) 12
BssT1I CCWWGG 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 1 cut(s) 269
BstACI GRCGYC 1 cut(s) 12
BstAUI TGTACA 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 16
BstF5I GGATG 2 cut(s) 31, 167
BstKTI GATC 2 cut(s) 32, 66
BstMBI GATC 2 cut(s) 29, 63
BstNI CCWGG 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 140
BsuRI GGCC 1 cut(s) 54
BtsCI GGATG 2 cut(s) 31, 167
Cac8I GCNNGC 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 144
CseI GACGC 1 cut(s) 20
Csp6I GTAC 2 cut(s) 126, 270
CviAII CATG 1 cut(s) 77
CviJI RGCY 3 cut(s) 54, 92, 123
CviKI_1 RGCY 3 cut(s) 54, 92, 123
CviQI GTAC 2 cut(s) 126, 270
DpnI GATC 2 cut(s) 31, 65
DpnII GATC 2 cut(s) 29, 63
Eco130I CCWWGG 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 144
EcoRII CCWGG 1 cut(s) 140
EcoT14I CCWWGG 1 cut(s) 100
EcoT22I ATGCAT 1 cut(s) 169
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 1 cut(s) 80
FaiI YATR 9 cut(s) 20, 22, 78, 113, 165, 175, 229, 231, 261
FatI CATG 1 cut(s) 76
FauNDI CATATG 2 cut(s) 20, 229
FokI GGATG 2 cut(s) 38, 154
FspBI CTAG 1 cut(s) 131
HaeIII GGCC 1 cut(s) 54
HgaI GACGC 1 cut(s) 20
Hin1I GRCGYC 1 cut(s) 12
Hin1II CATG 1 cut(s) 80
HinfI GANTC 1 cut(s) 275
HphI GGTGA 1 cut(s) 250
Hpy166II GTNNAC 1 cut(s) 128
Hpy188III TCNNGA 1 cut(s) 191
Hpy8I GTNNAC 1 cut(s) 128
HpyAV CCTTC 1 cut(s) 229
HpyCH4III ACNGT 1 cut(s) 269
HpyCH4V TGCA 3 cut(s) 80, 167, 309
Hsp92I GRCGYC 1 cut(s) 12
Hsp92II CATG 1 cut(s) 80
Kzo9I GATC 2 cut(s) 29, 63
LpnPI CCDG 7 cut(s) 28, 55, 105, 127, 154, 176, 315
LweI GCATC 1 cut(s) 176
MaeI CTAG 1 cut(s) 131
MalI GATC 2 cut(s) 31, 65
MboI GATC 2 cut(s) 29, 63
MluCI AATT 1 cut(s) 34
MnlI CCTC 2 cut(s) 44, 306
Mph1103I ATGCAT 1 cut(s) 169
MseI TTAA 1 cut(s) 153
MslI CAYNNNNRTG 1 cut(s) 178
MspR9I CCNGG 1 cut(s) 142
MvaI CCWGG 1 cut(s) 142
NdeI CATATG 2 cut(s) 20, 229
NdeII GATC 2 cut(s) 29, 63
NlaIII CATG 1 cut(s) 80
NlaIV GGNNCC 3 cut(s) 74, 185, 287
NsiI ATGCAT 1 cut(s) 169
PfeI GAWTC 1 cut(s) 275
PflMI CCANNNNNTGG 1 cut(s) 21
PfoI TCCNGGA 1 cut(s) 140
Psp6I CCWGG 1 cut(s) 140
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 3 cut(s) 74, 185, 287
PspPI GGNCC 1 cut(s) 144
PsrI GAACNNNNNNTAC 2 cut(s) 248, 280
RsaI GTAC 2 cut(s) 127, 271
RsaNI GTAC 2 cut(s) 126, 270
RseI CAYNNNNRTG 1 cut(s) 178
SaqAI TTAA 1 cut(s) 153
Sau3AI GATC 2 cut(s) 29, 63
Sau96I GGNCC 1 cut(s) 144
ScrFI CCNGG 1 cut(s) 142
SetI ASST 5 cut(s) 94, 240, 287, 298, 323
SfaNI GCATC 1 cut(s) 176
SinI GGWCC 1 cut(s) 144
SmiMI CAYNNNNRTG 1 cut(s) 178
Sse9I AATT 1 cut(s) 34
SspMI CTAG 1 cut(s) 131
StyD4I CCNGG 1 cut(s) 140
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 1 cut(s) 269
TaqI TCGA 1 cut(s) 66
TaqII GACCGA 1 cut(s) 161
TasI AATT 1 cut(s) 34
TatI WGTACW 2 cut(s) 125, 269
TfiI GAWTC 1 cut(s) 275
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TspDTI ATGAA 1 cut(s) 269
Van91I CCANNNNNTGG 1 cut(s) 21
VpaK11BI GGWCC 1 cut(s) 144
XspI CTAG 1 cut(s) 131
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.