RchiOBHm_Chr6g0284491

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001964
Physical Location & Seq
Reverse (-)
47722149 .. 47723342
1194 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25512

Sequence Viewer

Length: 534 bp
ATGCTTTGCAACCTTTCTGAATCGTACATGATGCGGCTTTCTTTGGTCTTCTTTCTAGCACTTGTTGTAATGAAACATACAAGTGAAGCCGCTGATAATGTTAGCTTTGATGAAACCTACGTCGTTCGATGCGGAAATGATCATATCTCGAAGCTAGACCAAGGAAGAGAAGTTCAGCTCTCTTTGGACTTCATTTCACATCCAGATAGTAAGCCTGGAAATCATGATGAGCTAGACTTTGAGTTCTTGGGCACAGATGGACCTGTATACTCTTTACAAACAAATATTTTCGCAAATGACAATGGGAAGAGAGAGCAAAGACTTCATCTTTGGTTTGATCCCACAAAGAATTTTCTTAAGTATGGAATCCTATGGAACCATCGCCAAGTAGTCTTTTTTGTGGACAACATCCCCATTAGAGTGTTCAAGAACAATACAAGGTTGGCTGCAAGGTACCCTTCACAACAGATGTCTGTGGAGGGAAGCATATGGAATATATGGAGAATCTTGGGCATCAAGTGGGAAAAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

177

Amino Acids

20.69

Weight (kDa)

6.06

Isoelectric Point (pI)

33.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 69 - 165 5.9e-30 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 453
AccB1I GGYRCC 1 cut(s) 453
AccI GTMKAC 1 cut(s) 267
AciI CCGC 3 cut(s) 34, 90, 132
AclWI GGATC 1 cut(s) 332
AcsI RAATTY 1 cut(s) 349
AfaI GTAC 2 cut(s) 26, 455
AflII CTTAAG 1 cut(s) 356
AgsI TTSAA 1 cut(s) 427
AjnI CCWGG 1 cut(s) 214
AloI GAACNNNNNNTCC 2 cut(s) 156, 188
AluBI AGCT 4 cut(s) 105, 154, 178, 232
AluI AGCT 4 cut(s) 105, 154, 178, 232
AlwI GGATC 1 cut(s) 332
ApeKI GCWGC 1 cut(s) 446
ApoI RAATTY 1 cut(s) 349
ArsI GACNNNNNNTTYG 2 cut(s) 312, 344
Asp718I GGTACC 1 cut(s) 453
AspS9I GGNCC 1 cut(s) 260
AvaII GGWCC 1 cut(s) 260
BaeGI GKGCMC 1 cut(s) 254
BanI GGYRCC 1 cut(s) 453
BbsI GAAGAC 1 cut(s) 40
BbvI GCAGC 1 cut(s) 433
BccI CCATC 2 cut(s) 251, 387
BciT130I CCWGG 1 cut(s) 216
BclI TGATCA 1 cut(s) 139
BfaI CTAG 3 cut(s) 56, 155, 233
BfrI CTTAAG 1 cut(s) 356
BisI GCNGC 3 cut(s) 35, 90, 447
BlsI GCNGC 3 cut(s) 36, 91, 448
Bme1390I CCNGG 1 cut(s) 216
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 1 cut(s) 260
BmiI GGNNCC 2 cut(s) 377, 455
BmrFI CCNGG 1 cut(s) 216
BmsI GCATC 3 cut(s) 21, 119, 522
BpiI GAAGAC 1 cut(s) 40
BsaJI CCNNGG 1 cut(s) 160
BseBI CCWGG 1 cut(s) 216
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 2 cut(s) 199, 408
BseSI GKGCMC 1 cut(s) 254
BseXI GCAGC 1 cut(s) 433
BshNI GGYRCC 1 cut(s) 453
Bsp1286I GDGCHC 1 cut(s) 254
Bsp143I GATC 2 cut(s) 139, 337
BspACI CCGC 3 cut(s) 34, 90, 132
BspHI TCATGA 1 cut(s) 223
BspLI GGNNCC 2 cut(s) 377, 455
BspPI GGATC 1 cut(s) 332
BspT107I GGYRCC 1 cut(s) 453
BspTI CTTAAG 1 cut(s) 356
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 2 cut(s) 139, 337
BssNAI GTATAC 1 cut(s) 268
BssT1I CCWWGG 1 cut(s) 160
Bst1107I GTATAC 1 cut(s) 268
Bst2UI CCWGG 1 cut(s) 216
Bst6I CTCTTC 2 cut(s) 160, 302
BstAFI CTTAAG 1 cut(s) 356
BstF5I GGATG 2 cut(s) 199, 408
BstKTI GATC 2 cut(s) 142, 340
BstMBI GATC 2 cut(s) 139, 337
BstNI CCWGG 1 cut(s) 216
BstSCI CCNGG 1 cut(s) 214
BstSLI GKGCMC 1 cut(s) 254
BstV1I GCAGC 1 cut(s) 433
BstV2I GAAGAC 1 cut(s) 40
BstZ17I GTATAC 1 cut(s) 268
BtgZI GCGATG 1 cut(s) 365
BtsCI GGATG 2 cut(s) 199, 408
CciI TCATGA 1 cut(s) 223
Cfr13I GGNCC 1 cut(s) 260
Csp6I GTAC 2 cut(s) 25, 454
CviAII CATG 2 cut(s) 28, 224
CviJI RGCY 8 cut(s) 37, 89, 105, 154, 178, 214, 232, 446
CviKI_1 RGCY 8 cut(s) 37, 89, 105, 154, 178, 214, 232, 446
CviQI GTAC 2 cut(s) 25, 454
DpnI GATC 2 cut(s) 141, 339
DpnII GATC 2 cut(s) 139, 337
Eam1104I CTCTTC 2 cut(s) 160, 302
EarI CTCTTC 2 cut(s) 160, 302
Eco130I CCWWGG 1 cut(s) 160
Eco47I GGWCC 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 160
ErhI CCWWGG 1 cut(s) 160
FaeI CATG 2 cut(s) 31, 227
FalI AAGNNNNNCTT 2 cut(s) 442, 474
FatI CATG 2 cut(s) 27, 223
FauNDI CATATG 1 cut(s) 488
FbaI TGATCA 1 cut(s) 139
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 3 cut(s) 35, 90, 447
FokI GGATG 2 cut(s) 186, 395
Fsp4HI GCNGC 3 cut(s) 35, 90, 447
FspBI CTAG 3 cut(s) 56, 155, 233
GluI GCNGC 3 cut(s) 35, 90, 447
Hin1II CATG 2 cut(s) 31, 227
HinfI GANTC 3 cut(s) 20, 366, 504
Hpy166II GTNNAC 2 cut(s) 268, 403
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 4 cut(s) 148, 203, 224, 427
Hpy8I GTNNAC 2 cut(s) 268, 403
Hpy99I CGWCG 1 cut(s) 125
HpyAV CCTTC 1 cut(s) 468
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 2 cut(s) 9, 449
HpySE526I ACGT 1 cut(s) 120
Hsp92II CATG 2 cut(s) 31, 227
KpnI GGTACC 1 cut(s) 457
Ksp22I TGATCA 1 cut(s) 139
Kzo9I GATC 2 cut(s) 139, 337
LpnPI CCDG 4 cut(s) 201, 216, 228, 276
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 3 cut(s) 21, 119, 522
MaeI CTAG 3 cut(s) 56, 155, 233
MaeII ACGT 1 cut(s) 120
MalI GATC 2 cut(s) 141, 339
MboI GATC 2 cut(s) 139, 337
MboII GAAGA 3 cut(s) 40, 177, 319
MhlI GDGCHC 1 cut(s) 254
MluCI AATT 1 cut(s) 349
MnlI CCTC 1 cut(s) 472
MseI TTAA 2 cut(s) 357, 532
MslI CAYNNNNRTG 2 cut(s) 81, 419
MspA1I CMGCKG 1 cut(s) 92
MspCI CTTAAG 1 cut(s) 356
MspR9I CCNGG 1 cut(s) 216
MvaI CCWGG 1 cut(s) 216
NdeI CATATG 1 cut(s) 488
NdeII GATC 2 cut(s) 139, 337
NlaIII CATG 2 cut(s) 31, 227
NlaIV GGNNCC 2 cut(s) 377, 455
PagI TCATGA 1 cut(s) 223
PfeI GAWTC 3 cut(s) 20, 366, 504
PkrI GCNGC 3 cut(s) 36, 91, 448
Psp6I CCWGG 1 cut(s) 214
PspGI CCWGG 1 cut(s) 214
PspN4I GGNNCC 2 cut(s) 377, 455
PspPI GGNCC 1 cut(s) 260
RsaI GTAC 2 cut(s) 26, 455
RsaNI GTAC 2 cut(s) 25, 454
RseI CAYNNNNRTG 2 cut(s) 81, 419
SaqAI TTAA 2 cut(s) 357, 532
SatI GCNGC 3 cut(s) 35, 90, 447
Sau3AI GATC 2 cut(s) 139, 337
Sau96I GGNCC 1 cut(s) 260
ScrFI CCNGG 1 cut(s) 216
SduI GDGCHC 1 cut(s) 254
SfaNI GCATC 3 cut(s) 21, 119, 522
SinI GGWCC 1 cut(s) 260
SmiMI CAYNNNNRTG 2 cut(s) 81, 419
SmlI CTYRAG 1 cut(s) 356
SmoI CTYRAG 1 cut(s) 356
Sse9I AATT 1 cut(s) 349
SsiI CCGC 3 cut(s) 34, 90, 132
SspI AATATT 1 cut(s) 286
SspMI CTAG 3 cut(s) 56, 155, 233
StyD4I CCNGG 1 cut(s) 214
StyI CCWWGG 1 cut(s) 160
TaiI ACGT 1 cut(s) 123
TaqI TCGA 2 cut(s) 127, 149
TasI AATT 1 cut(s) 349
TauI GCSGC 2 cut(s) 37, 92
TfiI GAWTC 3 cut(s) 20, 366, 504
Tru1I TTAA 2 cut(s) 357, 532
Tru9I TTAA 2 cut(s) 357, 532
TseI GCWGC 1 cut(s) 446
TspDTI ATGAA 4 cut(s) 86, 126, 181, 314
Vha464I CTTAAG 1 cut(s) 356
VpaK11BI GGWCC 1 cut(s) 260
XapI RAATTY 1 cut(s) 349
XmiI GTMKAC 1 cut(s) 267
XspI CTAG 3 cut(s) 56, 155, 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.